AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0366390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0366390 MtrunA17_Chr3g0107381 93.665 221 8 1 1 215 1 221 2.99e-153 424
Msa0366390 MtrunA17_Chr3g0110131 54.202 238 85 4 1 215 1 237 1.33e-74 225
Msa0366390 MtrunA17_Chr8g0351751 48.387 248 90 8 1 215 1 243 1.28e-62 195
Msa0366390 MtrunA17_Chr3g0107461 53.896 154 70 1 1 153 1 154 1.33e-55 174
Msa0366390 MtrunA17_Chr4g0014321 51.299 154 74 1 1 153 1 154 3.36e-53 170
Msa0366390 MtrunA17_Chr4g0013041 50.323 155 74 2 1 154 1 153 2.56e-51 165
Msa0366390 MtrunA17_Chr3g0107331 48.667 150 77 0 3 152 2 151 1.58e-50 161
Msa0366390 MtrunA17_Chr4g0014031 53.247 154 71 1 1 153 1 154 5.33e-50 162
Msa0366390 MtrunA17_Chr4g0014131 53.896 154 70 1 1 153 1 154 2.22e-48 158
Msa0366390 MtrunA17_Chr4g0014121 52.866 157 73 1 1 156 29 185 2.43e-48 156
Msa0366390 MtrunA17_Chr5g0432691 43.750 160 90 0 1 160 1 160 4.33e-48 155
Msa0366390 MtrunA17_Chr2g0296831 59.836 122 49 0 1 122 1 122 1.05e-46 150
Msa0366390 MtrunA17_Chr2g0283941 45.251 179 95 2 1 178 1 177 1.37e-46 154
Msa0366390 MtrunA17_Chr4g0031421 52.414 145 64 3 29 171 1 142 4.67e-43 141
Msa0366390 MtrunA17_Chr3g0107351 41.139 158 93 0 3 160 2 159 1.95e-41 138
Msa0366390 MtrunA17_Chr5g0420351 45.098 153 83 1 1 153 1 152 3.48e-41 137
Msa0366390 MtrunA17_Chr3g0107361 41.250 160 94 0 3 162 2 161 1.76e-40 135
Msa0366390 MtrunA17_Chr3g0105791 49.306 144 70 2 29 171 1 142 2.27e-40 135
Msa0366390 MtrunA17_Chr3g0107341 39.241 158 96 0 3 160 2 159 3.29e-38 130
Msa0366390 MtrunA17_Chr2g0296861 44.937 158 87 0 1 158 1 158 1.22e-36 125
Msa0366390 MtrunA17_Chr7g0218151 46.753 154 81 1 1 154 1 153 8.40e-36 124
Msa0366390 MtrunA17_Chr1g0188681 42.857 154 87 1 1 154 1 153 1.30e-34 120
Msa0366390 MtrunA17_Chr4g0012761 45.455 154 83 1 1 154 1 153 3.97e-34 119
Msa0366390 MtrunA17_Chr1g0197261 37.255 153 95 1 1 153 1 152 9.41e-34 118
Msa0366390 MtrunA17_Chr1g0193311 45.098 153 83 1 1 153 1 152 1.68e-33 117
Msa0366390 MtrunA17_Chr1g0197461 38.411 151 92 1 3 153 2 151 2.21e-33 117
Msa0366390 MtrunA17_Chr3g0106421 39.865 148 86 2 29 173 1 148 1.76e-32 117
Msa0366390 MtrunA17_Chr1g0188661 43.506 154 86 1 1 154 1 153 2.44e-32 115
Msa0366390 MtrunA17_Chr3g0106501 46.032 126 67 1 29 153 1 126 6.07e-31 111
Msa0366390 MtrunA17_Chr1g0188651 44.156 154 83 2 1 154 1 151 9.95e-30 108
Msa0366390 MtrunA17_Chr1g0197321 35.714 154 97 2 1 153 1 153 3.01e-29 107
Msa0366390 MtrunA17_Chr4g0008701 34.146 164 104 2 1 161 1 163 7.78e-24 98.6
Msa0366390 MtrunA17_Chr7g0267601 31.098 164 109 2 1 161 1 163 4.16e-22 93.6
Msa0366390 MtrunA17_Chr3g0128721 32.520 123 83 0 3 125 6 128 1.30e-19 86.7
Msa0366390 MtrunA17_Chr6g0457851 25.786 159 104 5 1 153 11 161 1.58e-15 74.3
Msa0366390 MtrunA17_Chr2g0296851 49.383 81 40 1 74 153 1 81 4.36e-15 68.2
Msa0366390 MtrunA17_Chr1g0212151 28.369 141 87 5 1 135 1 133 1.04e-14 71.6
Msa0366390 MtrunA17_Chr4g0022641 27.381 168 113 6 1 162 1 165 1.52e-13 68.6
Msa0366390 MtrunA17_Chr6g0449851 25.551 227 137 9 1 215 1 207 5.82e-13 66.6
Msa0366390 MtrunA17_Chr4g0059631 30.147 136 89 4 1 131 1 135 1.25e-12 65.1
Msa0366390 MtrunA17_Chr6g0449861 29.204 113 73 3 1 107 9 120 1.32e-12 65.9
Msa0366390 MtrunA17_Chr1g0212471 26.136 176 115 3 1 170 1 167 3.69e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0366390 AT5G48670.1 52.000 175 82 1 1 173 1 175 4.24e-54 176
Msa0366390 AT3G05860.2 39.801 201 113 3 1 195 1 199 2.37e-40 137
Msa0366390 AT3G05860.1 40.838 191 105 3 1 185 1 189 2.43e-39 136
Msa0366390 AT3G05860.3 41.758 182 92 4 1 176 1 174 7.40e-39 134
Msa0366390 AT1G65300.1 41.060 151 89 0 3 153 2 152 1.67e-37 132
Msa0366390 AT5G26650.1 34.597 211 127 4 4 203 2 212 1.22e-35 129
Msa0366390 AT5G27810.1 60.215 93 37 0 29 121 1 93 1.84e-35 122
Msa0366390 AT1G65330.1 39.735 151 91 0 3 153 2 152 9.02e-35 125
Msa0366390 AT1G31630.1 38.562 153 91 3 3 154 2 152 4.09e-34 124
Msa0366390 AT5G27960.1 33.175 211 130 4 4 203 2 212 2.48e-33 122
Msa0366390 AT1G31640.1 40.000 150 89 1 3 152 2 150 4.61e-33 124
Msa0366390 AT5G26630.1 41.176 153 90 0 1 153 1 153 1.33e-32 117
Msa0366390 AT2G28700.1 36.667 150 93 2 1 149 1 149 1.48e-28 109
Msa0366390 AT1G22590.2 36.025 161 94 2 1 158 1 155 7.42e-26 98.6
Msa0366390 AT5G06500.1 29.714 175 119 2 1 175 1 171 8.00e-23 92.8
Msa0366390 AT5G26580.1 33.333 153 88 1 1 153 1 139 1.27e-22 94.0
Msa0366390 AT2G40210.1 42.188 128 74 0 1 128 1 128 2.39e-22 93.6
Msa0366390 AT2G15660.1 29.412 119 77 2 38 153 31 145 6.50e-12 63.5
Msa0366390 AT5G55690.1 35.714 98 56 2 1 91 1 98 1.95e-11 62.4
Msa0366390 AT5G55690.2 35.714 98 56 2 1 91 1 98 1.95e-11 62.4

Find 60 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATTGTACCACATGGTTTC+TGG 0.167760 3_1:-61301486 Msa0366390:CDS
TCCCCTCCTCTTTGTGTCAC+TGG 0.360448 3_1:+61302769 None:intergenic
TCAATTGTTGAATTTATGTT+TGG 0.370267 3_1:-61301390 Msa0366390:three_prime_UTR
CTCCACTCACATTTCCAGCT+TGG 0.379872 3_1:+61301614 None:intergenic
AAATGTGAGTGGAGGGGAGC+AGG 0.400231 3_1:-61301605 Msa0366390:CDS
GAGTGGAGGGGAGCAGGGAA+TGG 0.407483 3_1:-61301599 Msa0366390:CDS
GAGAAAGAAATGAGTAAGAA+GGG 0.419191 3_1:+61302842 None:intergenic
TTCAAGAGACTTGTTGATCA+AGG 0.420086 3_1:+61301463 None:intergenic
AACAAAGGAATGACAACAAA+AGG 0.420798 3_1:-61301798 Msa0366390:CDS
AGAGAAAGAAATGAGTAAGA+AGG 0.424908 3_1:+61302841 None:intergenic
TTTAAATGTGTCCATGCTTA+GGG 0.431187 3_1:-61301347 Msa0366390:three_prime_UTR
GATGTGTTACCATTTGAAAA+TGG 0.433134 3_1:-61301511 Msa0366390:CDS
AATGTGAGTGGAGGGGAGCA+GGG 0.435248 3_1:-61301604 Msa0366390:CDS
GGCCAATGCTGCCTTTGCAT+TGG 0.439467 3_1:+61301562 None:intergenic
ACGATCCTCAACCAGAGGTT+TGG 0.446002 3_1:-61301954 Msa0366390:CDS
AGAGGTTTGGCCTTCGCCAT+GGG 0.456027 3_1:-61301941 Msa0366390:CDS
TTCGCCATGGGGAGTGCAAA+AGG 0.457021 3_1:-61301929 Msa0366390:CDS
GCTCAAGTTCGGGCAATCTC+CGG 0.458516 3_1:+61301893 None:intergenic
CACATCACCAATGTTGTTGT+TGG 0.473556 3_1:+61301528 None:intergenic
ATCCAATGCAAAGGCAGCAT+TGG 0.475495 3_1:-61301564 Msa0366390:CDS
CATGGCGAAGGCCAAACCTC+TGG 0.486204 3_1:+61301943 None:intergenic
CGTTCAGTCTCAAGCTGAAG+AGG 0.494904 3_1:-61301647 Msa0366390:CDS
CAGAGGTTTGGCCTTCGCCA+TGG 0.503800 3_1:-61301942 Msa0366390:CDS
AAAAGGTGCTGTCAAGGTTC+CGG 0.507965 3_1:-61301912 Msa0366390:CDS
CTTTGTGTCACTGGTGATGT+AGG 0.508282 3_1:+61302778 None:intergenic
AAGAGAGTTTCTTGAAGCAA+AGG 0.517056 3_1:-61301849 Msa0366390:CDS
AATGTGTCCATGCTTAGGGT+TGG 0.520246 3_1:-61301343 Msa0366390:three_prime_UTR
ATGTTTCAATGCCTTAATGC+TGG 0.536723 3_1:-61301757 Msa0366390:CDS
CGCTGCCATGACTAGGAAGA+AGG 0.539960 3_1:-61302810 Msa0366390:exon
TTCTCAACGCTGCCATGACT+AGG 0.541469 3_1:-61302817 Msa0366390:exon
GATTTCACCAACAACAACAT+TGG 0.549412 3_1:-61301535 Msa0366390:CDS
ATGAAATCAGCACGCTCTGT+GGG 0.552209 3_1:-61302008 Msa0366390:CDS
AGAACTTGAAACAGATCGAT+AGG 0.558346 3_1:-61301675 Msa0366390:CDS
GAGTGCAAAAGGTGCTGTCA+AGG 0.560319 3_1:-61301918 Msa0366390:CDS
GAGGTTGTTCAAAACCAAGC+TGG 0.560495 3_1:-61301628 Msa0366390:CDS
AAGCTGGAAATGTGAGTGGA+GGG 0.564199 3_1:-61301612 Msa0366390:CDS
GCTCACCTTCTTCCTAGTCA+TGG 0.576647 3_1:+61302805 None:intergenic
CACCAGTGACACAAAGAGGA+GGG 0.584600 3_1:-61302771 Msa0366390:CDS
GAAGGCCAAACCTCTGGTTG+AGG 0.594220 3_1:+61301949 None:intergenic
GCAAAGGCAGCATTGGCCAA+TGG 0.595451 3_1:-61301557 Msa0366390:CDS
TTGTTGTTGGTGAAATCCAT+TGG 0.595560 3_1:+61301541 None:intergenic
AAGAACAGCTGAAGAAACAA+AGG 0.602104 3_1:-61301813 Msa0366390:CDS
AAGGAATGACAACAAAAGGA+AGG 0.606288 3_1:-61301794 Msa0366390:CDS
TGTCACTGGTGATGTAGGCA+AGG 0.608234 3_1:+61302783 None:intergenic
AGCTGGAAATGTGAGTGGAG+GGG 0.617771 3_1:-61301611 Msa0366390:CDS
CAAGCTGGAAATGTGAGTGG+AGG 0.625495 3_1:-61301613 Msa0366390:CDS
GAGGTTTGGCCTTCGCCATG+GGG 0.626589 3_1:-61301940 Msa0366390:CDS
ACCAGTGACACAAAGAGGAG+GGG 0.628101 3_1:-61302770 Msa0366390:CDS
ACATCACCAGTGACACAAAG+AGG 0.631331 3_1:-61302775 Msa0366390:CDS
CCTGTGTCCAACCCTAAGCA+TGG 0.640879 3_1:+61301336 None:intergenic
AATGGTAACATTGTACCACA+TGG 0.641540 3_1:-61301493 Msa0366390:CDS
AACCAAGCTGGAAATGTGAG+TGG 0.641568 3_1:-61301616 Msa0366390:CDS
ATGATCTCAATGATATCTCA+TGG 0.651968 3_1:-61301708 Msa0366390:CDS
ACATTGCTGATCCAATGCAA+AGG 0.652826 3_1:-61301573 Msa0366390:CDS
CCATGCTTAGGGTTGGACAC+AGG 0.655939 3_1:-61301336 Msa0366390:three_prime_UTR
TGAGATCATCCATGCCAACA+TGG 0.658598 3_1:+61301722 None:intergenic
TCAAAACGATCCTCAACCAG+AGG 0.673462 3_1:-61301959 Msa0366390:CDS
GATGAAATCAGCACGCTCTG+TGG 0.675321 3_1:-61302009 Msa0366390:CDS
TCACCAGTGACACAAAGAGG+AGG 0.677630 3_1:-61302772 Msa0366390:CDS
GATCAAGGCCAGAAACCATG+TGG 0.739110 3_1:+61301478 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAATAAAATAAAAATT+GGG + chr3_1:61301887-61301906 None:intergenic 0.0%
!! TATTTAAATAAAATAAAAAT+TGG + chr3_1:61301888-61301907 None:intergenic 0.0%
!! ATTTAAATAAAATAAAAATT+GGG + chr3_1:61301887-61301906 None:intergenic 0.0%
!! TATTTAAATAAAATAAAAAT+TGG + chr3_1:61301888-61301907 None:intergenic 0.0%
!!! TAGTTTTTATCATTTTTCAA+TGG + chr3_1:61301563-61301582 None:intergenic 15.0%
!!! AGATTTCATTTTGTTTTTTT+TGG - chr3_1:61301820-61301839 Msa0366390:CDS 15.0%
!!! TGAAATTATTTTTATCTCAT+TGG - chr3_1:61301955-61301974 Msa0366390:CDS 15.0%
!!! TTATGTTTGGTTTTATTTTA+TGG - chr3_1:61302793-61302812 Msa0366390:CDS 15.0%
!!! TAGTTTTTATCATTTTTCAA+TGG + chr3_1:61301563-61301582 None:intergenic 15.0%
!!! AGATTTCATTTTGTTTTTTT+TGG - chr3_1:61301820-61301839 Msa0366390:CDS 15.0%
!!! TGAAATTATTTTTATCTCAT+TGG - chr3_1:61301955-61301974 Msa0366390:CDS 15.0%
!!! TTATGTTTGGTTTTATTTTA+TGG - chr3_1:61302793-61302812 Msa0366390:CDS 15.0%
!! ACATTCAAGAAAAGAAAAAA+TGG - chr3_1:61301424-61301443 Msa0366390:three_prime_UTR 20.0%
!! TCAATTGTTGAATTTATGTT+TGG - chr3_1:61302780-61302799 Msa0366390:CDS 20.0%
!! ACATTCAAGAAAAGAAAAAA+TGG - chr3_1:61301424-61301443 Msa0366390:three_prime_UTR 20.0%
!! TCAATTGTTGAATTTATGTT+TGG - chr3_1:61302780-61302799 Msa0366390:CDS 20.0%
! AATGGTAATTACAACATGTA+TGG + chr3_1:61301545-61301564 None:intergenic 25.0%
! TAGATCGTAAATTGAATCAA+TGG - chr3_1:61301637-61301656 Msa0366390:CDS 25.0%
! AGATCGTAAATTGAATCAAT+GGG - chr3_1:61301638-61301657 Msa0366390:CDS 25.0%
! TTAGTGGCAAACAAATTTAT+AGG + chr3_1:61301790-61301809 None:intergenic 25.0%
! AAATCTCTCACTTAATTTAG+TGG + chr3_1:61301806-61301825 None:intergenic 25.0%
!!! AGAGATTTCATTTTGTTGTT+TGG - chr3_1:61301848-61301867 Msa0366390:CDS 25.0%
!!! TTTTTATCTCATTGGTGTTT+CGG - chr3_1:61301963-61301982 Msa0366390:CDS 25.0%
! AATGATTGAAACAACAAAGT+AGG + chr3_1:61302093-61302112 None:intergenic 25.0%
!!! CTTGTTGTATTTTTTATACC+AGG - chr3_1:61302122-61302141 Msa0366390:intron 25.0%
!! ACAATGTTACCATTTTCAAA+TGG + chr3_1:61302671-61302690 None:intergenic 25.0%
! AATGGTAATTACAACATGTA+TGG + chr3_1:61301545-61301564 None:intergenic 25.0%
! TAGATCGTAAATTGAATCAA+TGG - chr3_1:61301637-61301656 Msa0366390:CDS 25.0%
! AGATCGTAAATTGAATCAAT+GGG - chr3_1:61301638-61301657 Msa0366390:CDS 25.0%
! TTAGTGGCAAACAAATTTAT+AGG + chr3_1:61301790-61301809 None:intergenic 25.0%
! AAATCTCTCACTTAATTTAG+TGG + chr3_1:61301806-61301825 None:intergenic 25.0%
!!! AGAGATTTCATTTTGTTGTT+TGG - chr3_1:61301848-61301867 Msa0366390:CDS 25.0%
!!! TTTTTATCTCATTGGTGTTT+CGG - chr3_1:61301963-61301982 Msa0366390:CDS 25.0%
! AATGATTGAAACAACAAAGT+AGG + chr3_1:61302093-61302112 None:intergenic 25.0%
!!! CTTGTTGTATTTTTTATACC+AGG - chr3_1:61302122-61302141 Msa0366390:intron 25.0%
!! ACAATGTTACCATTTTCAAA+TGG + chr3_1:61302671-61302690 None:intergenic 25.0%
GAGAAAGAAATGAGTAAGAA+GGG + chr3_1:61301331-61301350 None:intergenic 30.0%
AGAGAAAGAAATGAGTAAGA+AGG + chr3_1:61301332-61301351 None:intergenic 30.0%
ATGCAAGATTGATATAGACA+TGG + chr3_1:61301482-61301501 None:intergenic 30.0%
GATCGTAAATTGAATCAATG+GGG - chr3_1:61301639-61301658 Msa0366390:CDS 30.0%
ACTTTACTTTACGATGTTGT+GGG + chr3_1:61301693-61301712 None:intergenic 30.0%
AACTTTACTTTACGATGTTG+TGG + chr3_1:61301694-61301713 None:intergenic 30.0%
!!! TTTCATTTTGTTGTTTGGCT+TGG - chr3_1:61301853-61301872 Msa0366390:CDS 30.0%
ATTTAACATGTTTGTCCCTT+TGG - chr3_1:61301909-61301928 Msa0366390:CDS 30.0%
!! TTTTATCTCATTGGTGTTTC+GGG - chr3_1:61301964-61301983 Msa0366390:CDS 30.0%
!!! TTTTTTGCTTTGCTCAAGTT+CGG + chr3_1:61302291-61302310 None:intergenic 30.0%
AACAAAGGAATGACAACAAA+AGG - chr3_1:61302372-61302391 Msa0366390:intron 30.0%
ATGATCTCAATGATATCTCA+TGG - chr3_1:61302462-61302481 Msa0366390:intron 30.0%
GATGTGTTACCATTTGAAAA+TGG - chr3_1:61302659-61302678 Msa0366390:intron 30.0%
! TTTTAAATGTGTCCATGCTT+AGG - chr3_1:61302822-61302841 Msa0366390:exon 30.0%
TTTAAATGTGTCCATGCTTA+GGG - chr3_1:61302823-61302842 Msa0366390:exon 30.0%
GAGAAAGAAATGAGTAAGAA+GGG + chr3_1:61301331-61301350 None:intergenic 30.0%
AGAGAAAGAAATGAGTAAGA+AGG + chr3_1:61301332-61301351 None:intergenic 30.0%
ATGCAAGATTGATATAGACA+TGG + chr3_1:61301482-61301501 None:intergenic 30.0%
GATCGTAAATTGAATCAATG+GGG - chr3_1:61301639-61301658 Msa0366390:CDS 30.0%
ACTTTACTTTACGATGTTGT+GGG + chr3_1:61301693-61301712 None:intergenic 30.0%
AACTTTACTTTACGATGTTG+TGG + chr3_1:61301694-61301713 None:intergenic 30.0%
!!! TTTCATTTTGTTGTTTGGCT+TGG - chr3_1:61301853-61301872 Msa0366390:CDS 30.0%
ATTTAACATGTTTGTCCCTT+TGG - chr3_1:61301909-61301928 Msa0366390:CDS 30.0%
!! TTTTATCTCATTGGTGTTTC+GGG - chr3_1:61301964-61301983 Msa0366390:CDS 30.0%
!!! TTTTTTGCTTTGCTCAAGTT+CGG + chr3_1:61302291-61302310 None:intergenic 30.0%
AACAAAGGAATGACAACAAA+AGG - chr3_1:61302372-61302391 Msa0366390:intron 30.0%
ATGATCTCAATGATATCTCA+TGG - chr3_1:61302462-61302481 Msa0366390:intron 30.0%
GATGTGTTACCATTTGAAAA+TGG - chr3_1:61302659-61302678 Msa0366390:intron 30.0%
! TTTTAAATGTGTCCATGCTT+AGG - chr3_1:61302822-61302841 Msa0366390:exon 30.0%
TTTAAATGTGTCCATGCTTA+GGG - chr3_1:61302823-61302842 Msa0366390:exon 30.0%
AAATTGAATCAATGGGGAGT+TGG - chr3_1:61301645-61301664 Msa0366390:CDS 35.0%
AATTGAATCAATGGGGAGTT+GGG - chr3_1:61301646-61301665 Msa0366390:CDS 35.0%
TCAATCTTCTTGATCAAACC+TGG + chr3_1:61302143-61302162 None:intergenic 35.0%
! TTTTTGCTTTGCTCAAGTTC+GGG + chr3_1:61302290-61302309 None:intergenic 35.0%
AAGAGAGTTTCTTGAAGCAA+AGG - chr3_1:61302321-61302340 Msa0366390:intron 35.0%
AAGAACAGCTGAAGAAACAA+AGG - chr3_1:61302357-61302376 Msa0366390:intron 35.0%
AAGGAATGACAACAAAAGGA+AGG - chr3_1:61302376-61302395 Msa0366390:intron 35.0%
ATGTTTCAATGCCTTAATGC+TGG - chr3_1:61302413-61302432 Msa0366390:intron 35.0%
CAAAAATCTGACCAGCATTA+AGG + chr3_1:61302427-61302446 None:intergenic 35.0%
AGAACTTGAAACAGATCGAT+AGG - chr3_1:61302495-61302514 Msa0366390:intron 35.0%
! TTGTTGTTGGTGAAATCCAT+TGG + chr3_1:61302632-61302651 None:intergenic 35.0%
GATTTCACCAACAACAACAT+TGG - chr3_1:61302635-61302654 Msa0366390:intron 35.0%
AATGGTAACATTGTACCACA+TGG - chr3_1:61302677-61302696 Msa0366390:intron 35.0%
!! TTCAAGAGACTTGTTGATCA+AGG + chr3_1:61302710-61302729 None:intergenic 35.0%
!! GATCTGCTTTGTTGTGTTTT+AGG - chr3_1:61302744-61302763 Msa0366390:intron 35.0%
AAATTGAATCAATGGGGAGT+TGG - chr3_1:61301645-61301664 Msa0366390:CDS 35.0%
AATTGAATCAATGGGGAGTT+GGG - chr3_1:61301646-61301665 Msa0366390:CDS 35.0%
TCAATCTTCTTGATCAAACC+TGG + chr3_1:61302143-61302162 None:intergenic 35.0%
! TTTTTGCTTTGCTCAAGTTC+GGG + chr3_1:61302290-61302309 None:intergenic 35.0%
AAGAGAGTTTCTTGAAGCAA+AGG - chr3_1:61302321-61302340 Msa0366390:intron 35.0%
AAGAACAGCTGAAGAAACAA+AGG - chr3_1:61302357-61302376 Msa0366390:intron 35.0%
AAGGAATGACAACAAAAGGA+AGG - chr3_1:61302376-61302395 Msa0366390:intron 35.0%
ATGTTTCAATGCCTTAATGC+TGG - chr3_1:61302413-61302432 Msa0366390:intron 35.0%
CAAAAATCTGACCAGCATTA+AGG + chr3_1:61302427-61302446 None:intergenic 35.0%
AGAACTTGAAACAGATCGAT+AGG - chr3_1:61302495-61302514 Msa0366390:intron 35.0%
! TTGTTGTTGGTGAAATCCAT+TGG + chr3_1:61302632-61302651 None:intergenic 35.0%
GATTTCACCAACAACAACAT+TGG - chr3_1:61302635-61302654 Msa0366390:intron 35.0%
AATGGTAACATTGTACCACA+TGG - chr3_1:61302677-61302696 Msa0366390:intron 35.0%
!! TTCAAGAGACTTGTTGATCA+AGG + chr3_1:61302710-61302729 None:intergenic 35.0%
!! GATCTGCTTTGTTGTGTTTT+AGG - chr3_1:61302744-61302763 Msa0366390:intron 35.0%
GTTCAAATCCCAGTTGTGAA+AGG - chr3_1:61301711-61301730 Msa0366390:CDS 40.0%
TATGAGCACCTTTCACAACT+GGG + chr3_1:61301722-61301741 None:intergenic 40.0%
ATATGAGCACCTTTCACAAC+TGG + chr3_1:61301723-61301742 None:intergenic 40.0%
! TCAGCTGTTCTTGAGCTTTT+TGG + chr3_1:61302349-61302368 None:intergenic 40.0%
! GGTCAGATTTTTGACCATGT+TGG - chr3_1:61302434-61302453 Msa0366390:intron 40.0%
! GATTTTTGACCATGTTGGCA+TGG - chr3_1:61302439-61302458 Msa0366390:intron 40.0%
ACATTGCTGATCCAATGCAA+AGG - chr3_1:61302597-61302616 Msa0366390:intron 40.0%
CACATCACCAATGTTGTTGT+TGG + chr3_1:61302645-61302664 None:intergenic 40.0%
ACATTGTACCACATGGTTTC+TGG - chr3_1:61302684-61302703 Msa0366390:intron 40.0%
GTTCAAATCCCAGTTGTGAA+AGG - chr3_1:61301711-61301730 Msa0366390:CDS 40.0%
TATGAGCACCTTTCACAACT+GGG + chr3_1:61301722-61301741 None:intergenic 40.0%
ATATGAGCACCTTTCACAAC+TGG + chr3_1:61301723-61301742 None:intergenic 40.0%
! TCAGCTGTTCTTGAGCTTTT+TGG + chr3_1:61302349-61302368 None:intergenic 40.0%
! GGTCAGATTTTTGACCATGT+TGG - chr3_1:61302434-61302453 Msa0366390:intron 40.0%
! GATTTTTGACCATGTTGGCA+TGG - chr3_1:61302439-61302458 Msa0366390:intron 40.0%
ACATTGCTGATCCAATGCAA+AGG - chr3_1:61302597-61302616 Msa0366390:intron 40.0%
CACATCACCAATGTTGTTGT+TGG + chr3_1:61302645-61302664 None:intergenic 40.0%
ACATTGTACCACATGGTTTC+TGG - chr3_1:61302684-61302703 Msa0366390:intron 40.0%
! CTTTGTGTCACTGGTGATGT+AGG + chr3_1:61301395-61301414 None:intergenic 45.0%
ACATCACCAGTGACACAAAG+AGG - chr3_1:61301395-61301414 Msa0366390:three_prime_UTR 45.0%
CACACAGACGCTATTCCAAA+GGG + chr3_1:61301927-61301946 None:intergenic 45.0%
TCACACAGACGCTATTCCAA+AGG + chr3_1:61301928-61301947 None:intergenic 45.0%
ATGAAATCAGCACGCTCTGT+GGG - chr3_1:61302162-61302181 Msa0366390:intron 45.0%
TCAAAACGATCCTCAACCAG+AGG - chr3_1:61302211-61302230 Msa0366390:intron 45.0%
!! AAAAGGTGCTGTCAAGGTTC+CGG - chr3_1:61302258-61302277 Msa0366390:intron 45.0%
TGAGATCATCCATGCCAACA+TGG + chr3_1:61302451-61302470 None:intergenic 45.0%
GAGGTTGTTCAAAACCAAGC+TGG - chr3_1:61302542-61302561 Msa0366390:intron 45.0%
AACCAAGCTGGAAATGTGAG+TGG - chr3_1:61302554-61302573 Msa0366390:intron 45.0%
AAGCTGGAAATGTGAGTGGA+GGG - chr3_1:61302558-61302577 Msa0366390:intron 45.0%
ATCCAATGCAAAGGCAGCAT+TGG - chr3_1:61302606-61302625 Msa0366390:intron 45.0%
AATGTGTCCATGCTTAGGGT+TGG - chr3_1:61302827-61302846 Msa0366390:five_prime_UTR 45.0%
! CTTTGTGTCACTGGTGATGT+AGG + chr3_1:61301395-61301414 None:intergenic 45.0%
ACATCACCAGTGACACAAAG+AGG - chr3_1:61301395-61301414 Msa0366390:three_prime_UTR 45.0%
CACACAGACGCTATTCCAAA+GGG + chr3_1:61301927-61301946 None:intergenic 45.0%
TCACACAGACGCTATTCCAA+AGG + chr3_1:61301928-61301947 None:intergenic 45.0%
ATGAAATCAGCACGCTCTGT+GGG - chr3_1:61302162-61302181 Msa0366390:intron 45.0%
TCAAAACGATCCTCAACCAG+AGG - chr3_1:61302211-61302230 Msa0366390:intron 45.0%
!! AAAAGGTGCTGTCAAGGTTC+CGG - chr3_1:61302258-61302277 Msa0366390:intron 45.0%
TGAGATCATCCATGCCAACA+TGG + chr3_1:61302451-61302470 None:intergenic 45.0%
GAGGTTGTTCAAAACCAAGC+TGG - chr3_1:61302542-61302561 Msa0366390:intron 45.0%
AACCAAGCTGGAAATGTGAG+TGG - chr3_1:61302554-61302573 Msa0366390:intron 45.0%
AAGCTGGAAATGTGAGTGGA+GGG - chr3_1:61302558-61302577 Msa0366390:intron 45.0%
ATCCAATGCAAAGGCAGCAT+TGG - chr3_1:61302606-61302625 Msa0366390:intron 45.0%
AATGTGTCCATGCTTAGGGT+TGG - chr3_1:61302827-61302846 Msa0366390:five_prime_UTR 45.0%
!! TTTAAATAAAATAAAAATTG+GGG + chr3_1:61301886-61301905 None:intergenic 5.0%
!! TTTAAATAAAATAAAAATTG+GGG + chr3_1:61301886-61301905 None:intergenic 5.0%
TTCTCAACGCTGCCATGACT+AGG - chr3_1:61301353-61301372 Msa0366390:three_prime_UTR 50.0%
GCTCACCTTCTTCCTAGTCA+TGG + chr3_1:61301368-61301387 None:intergenic 50.0%
! TGTCACTGGTGATGTAGGCA+AGG + chr3_1:61301390-61301409 None:intergenic 50.0%
TCACCAGTGACACAAAGAGG+AGG - chr3_1:61301398-61301417 Msa0366390:three_prime_UTR 50.0%
CACCAGTGACACAAAGAGGA+GGG - chr3_1:61301399-61301418 Msa0366390:three_prime_UTR 50.0%
ACCAGTGACACAAAGAGGAG+GGG - chr3_1:61301400-61301419 Msa0366390:three_prime_UTR 50.0%
GGGAGTTGGGTCTAGCAAAA+TGG - chr3_1:61301659-61301678 Msa0366390:CDS 50.0%
GATGAAATCAGCACGCTCTG+TGG - chr3_1:61302161-61302180 Msa0366390:intron 50.0%
ACGATCCTCAACCAGAGGTT+TGG - chr3_1:61302216-61302235 Msa0366390:intron 50.0%
!! GAGTGCAAAAGGTGCTGTCA+AGG - chr3_1:61302252-61302271 Msa0366390:intron 50.0%
CGTTCAGTCTCAAGCTGAAG+AGG - chr3_1:61302523-61302542 Msa0366390:intron 50.0%
CTCCACTCACATTTCCAGCT+TGG + chr3_1:61302559-61302578 None:intergenic 50.0%
CAAGCTGGAAATGTGAGTGG+AGG - chr3_1:61302557-61302576 Msa0366390:intron 50.0%
AGCTGGAAATGTGAGTGGAG+GGG - chr3_1:61302559-61302578 Msa0366390:intron 50.0%
GATCAAGGCCAGAAACCATG+TGG + chr3_1:61302695-61302714 None:intergenic 50.0%
TTCTCAACGCTGCCATGACT+AGG - chr3_1:61301353-61301372 Msa0366390:three_prime_UTR 50.0%
GCTCACCTTCTTCCTAGTCA+TGG + chr3_1:61301368-61301387 None:intergenic 50.0%
! TGTCACTGGTGATGTAGGCA+AGG + chr3_1:61301390-61301409 None:intergenic 50.0%
TCACCAGTGACACAAAGAGG+AGG - chr3_1:61301398-61301417 Msa0366390:three_prime_UTR 50.0%
CACCAGTGACACAAAGAGGA+GGG - chr3_1:61301399-61301418 Msa0366390:three_prime_UTR 50.0%
ACCAGTGACACAAAGAGGAG+GGG - chr3_1:61301400-61301419 Msa0366390:three_prime_UTR 50.0%
GGGAGTTGGGTCTAGCAAAA+TGG - chr3_1:61301659-61301678 Msa0366390:CDS 50.0%
GATGAAATCAGCACGCTCTG+TGG - chr3_1:61302161-61302180 Msa0366390:intron 50.0%
ACGATCCTCAACCAGAGGTT+TGG - chr3_1:61302216-61302235 Msa0366390:intron 50.0%
!! GAGTGCAAAAGGTGCTGTCA+AGG - chr3_1:61302252-61302271 Msa0366390:intron 50.0%
CGTTCAGTCTCAAGCTGAAG+AGG - chr3_1:61302523-61302542 Msa0366390:intron 50.0%
CTCCACTCACATTTCCAGCT+TGG + chr3_1:61302559-61302578 None:intergenic 50.0%
CAAGCTGGAAATGTGAGTGG+AGG - chr3_1:61302557-61302576 Msa0366390:intron 50.0%
AGCTGGAAATGTGAGTGGAG+GGG - chr3_1:61302559-61302578 Msa0366390:intron 50.0%
GATCAAGGCCAGAAACCATG+TGG + chr3_1:61302695-61302714 None:intergenic 50.0%
CGCTGCCATGACTAGGAAGA+AGG - chr3_1:61301360-61301379 Msa0366390:three_prime_UTR 55.0%
TCCCCTCCTCTTTGTGTCAC+TGG + chr3_1:61301404-61301423 None:intergenic 55.0%
GAAGGCCAAACCTCTGGTTG+AGG + chr3_1:61302224-61302243 None:intergenic 55.0%
! AGAGGTTTGGCCTTCGCCAT+GGG - chr3_1:61302229-61302248 Msa0366390:intron 55.0%
! TTTTGCACTCCCCATGGCGA+AGG + chr3_1:61302242-61302261 None:intergenic 55.0%
! TTCGCCATGGGGAGTGCAAA+AGG - chr3_1:61302241-61302260 Msa0366390:intron 55.0%
! AGCACCTTTTGCACTCCCCA+TGG + chr3_1:61302248-61302267 None:intergenic 55.0%
GCTCAAGTTCGGGCAATCTC+CGG + chr3_1:61302280-61302299 None:intergenic 55.0%
AAATGTGAGTGGAGGGGAGC+AGG - chr3_1:61302565-61302584 Msa0366390:intron 55.0%
AATGTGAGTGGAGGGGAGCA+GGG - chr3_1:61302566-61302585 Msa0366390:intron 55.0%
GGCCAATGCTGCCTTTGCAT+TGG + chr3_1:61302611-61302630 None:intergenic 55.0%
!! GCAAAGGCAGCATTGGCCAA+TGG - chr3_1:61302613-61302632 Msa0366390:intron 55.0%
CCTGTGTCCAACCCTAAGCA+TGG + chr3_1:61302837-61302856 None:intergenic 55.0%
CCATGCTTAGGGTTGGACAC+AGG - chr3_1:61302834-61302853 Msa0366390:five_prime_UTR 55.0%
CGCTGCCATGACTAGGAAGA+AGG - chr3_1:61301360-61301379 Msa0366390:three_prime_UTR 55.0%
TCCCCTCCTCTTTGTGTCAC+TGG + chr3_1:61301404-61301423 None:intergenic 55.0%
GAAGGCCAAACCTCTGGTTG+AGG + chr3_1:61302224-61302243 None:intergenic 55.0%
! AGAGGTTTGGCCTTCGCCAT+GGG - chr3_1:61302229-61302248 Msa0366390:intron 55.0%
! TTTTGCACTCCCCATGGCGA+AGG + chr3_1:61302242-61302261 None:intergenic 55.0%
! TTCGCCATGGGGAGTGCAAA+AGG - chr3_1:61302241-61302260 Msa0366390:intron 55.0%
! AGCACCTTTTGCACTCCCCA+TGG + chr3_1:61302248-61302267 None:intergenic 55.0%
GCTCAAGTTCGGGCAATCTC+CGG + chr3_1:61302280-61302299 None:intergenic 55.0%
AAATGTGAGTGGAGGGGAGC+AGG - chr3_1:61302565-61302584 Msa0366390:intron 55.0%
AATGTGAGTGGAGGGGAGCA+GGG - chr3_1:61302566-61302585 Msa0366390:intron 55.0%
GGCCAATGCTGCCTTTGCAT+TGG + chr3_1:61302611-61302630 None:intergenic 55.0%
!! GCAAAGGCAGCATTGGCCAA+TGG - chr3_1:61302613-61302632 Msa0366390:intron 55.0%
CCTGTGTCCAACCCTAAGCA+TGG + chr3_1:61302837-61302856 None:intergenic 55.0%
CCATGCTTAGGGTTGGACAC+AGG - chr3_1:61302834-61302853 Msa0366390:five_prime_UTR 55.0%
CATGGCGAAGGCCAAACCTC+TGG + chr3_1:61302230-61302249 None:intergenic 60.0%
! CAGAGGTTTGGCCTTCGCCA+TGG - chr3_1:61302228-61302247 Msa0366390:intron 60.0%
! GAGGTTTGGCCTTCGCCATG+GGG - chr3_1:61302230-61302249 Msa0366390:intron 60.0%
CATGGCGAAGGCCAAACCTC+TGG + chr3_1:61302230-61302249 None:intergenic 60.0%
! CAGAGGTTTGGCCTTCGCCA+TGG - chr3_1:61302228-61302247 Msa0366390:intron 60.0%
! GAGGTTTGGCCTTCGCCATG+GGG - chr3_1:61302230-61302249 Msa0366390:intron 60.0%
GAGTGGAGGGGAGCAGGGAA+TGG - chr3_1:61302571-61302590 Msa0366390:intron 65.0%
GAGTGGAGGGGAGCAGGGAA+TGG - chr3_1:61302571-61302590 Msa0366390:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 61301320 61302872 61301320 ID=Msa0366390;Name=Msa0366390
chr3_1 mRNA 61301320 61302872 61301320 ID=Msa0366390-mRNA-1;Parent=Msa0366390;Name=Msa0366390-mRNA-1;_AED=0.01;_eAED=0.01;_QI=47|1|1|1|1|1|2|160|215
chr3_1 exon 61301320 61302048 61301320 ID=Msa0366390-mRNA-1:exon:11872;Parent=Msa0366390-mRNA-1
chr3_1 exon 61302747 61302872 61302747 ID=Msa0366390-mRNA-1:exon:11871;Parent=Msa0366390-mRNA-1
chr3_1 five_prime_UTR 61302826 61302872 61302826 ID=Msa0366390-mRNA-1:five_prime_utr;Parent=Msa0366390-mRNA-1
chr3_1 CDS 61302747 61302825 61302747 ID=Msa0366390-mRNA-1:cds;Parent=Msa0366390-mRNA-1
chr3_1 CDS 61301480 61302048 61301480 ID=Msa0366390-mRNA-1:cds;Parent=Msa0366390-mRNA-1
chr3_1 three_prime_UTR 61301320 61301479 61301320 ID=Msa0366390-mRNA-1:three_prime_utr;Parent=Msa0366390-mRNA-1
Gene Sequence

>Msa0366390

ATGACTAGGAAGAAGGTGAGCCTTGCCTACATCACCAGTGACACAAAGAGGAGGGGAACATTCAAGAAAAGAAAAAATGGTTTGATCAAGAAGATTGATGAAATCAGCACGCTCTGTGGGATCGAAGCGTGTGCTATCATCTTTGATCAAAACGATCCTCAACCAGAGGTTTGGCCTTCGCCATGGGGAGTGCAAAAGGTGCTGTCAAGGTTCCGGAGATTGCCCGAACTTGAGCAAAGCAAAAAAATGTTCAATCAAGAGAGTTTCTTGAAGCAAAGGATCCAAAAAGCTCAAGAACAGCTGAAGAAACAAAGGAATGACAACAAAAGGAAGGAAATGACTCATCTGATGTTTCAATGCCTTAATGCTGGTCAGATTTTTGACCATGTTGGCATGGATGATCTCAATGATATCTCATGGTTGATTGATCAGAACTTGAAACAGATCGATAGGAAACTCGTTCAGTCTCAAGCTGAAGAGGTTGTTCAAAACCAAGCTGGAAATGTGAGTGGAGGGGAGCAGGGAATGGAGAACATTGCTGATCCAATGCAAAGGCAGCATTGGCCAATGGATTTCACCAACAACAACATTGGTGATGTGTTACCATTTGAAAATGGTAACATTGTACCACATGGTTTCTGGCCTTGA
Protein sequence

>Msa0366390

MTRKKVSLAYITSDTKRRGTFKKRKNGLIKKIDEISTLCGIEACAIIFDQNDPQPEVWPSPWGVQKVLSRFRRLPELEQSKKMFNQESFLKQRIQKAQEQLKKQRNDNKRKEMTHLMFQCLNAGQIFDHVGMDDLNDISWLIDQNLKQIDRKLVQSQAEEVVQNQAGNVSGGEQGMENIADPMQRQHWPMDFTNNNIGDVLPFENGNIVPHGFWP*