AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0369070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0369070 MtrunA17_Chr3g0113121 92.130 216 17 0 30 245 1 216 9.57e-140 390
Msa0369070 MtrunA17_Chr5g0436501 41.401 157 78 6 50 200 48 196 8.15e-21 88.6
Msa0369070 MtrunA17_Chr5g0429521 37.209 129 68 2 78 200 80 201 1.78e-17 80.1
Msa0369070 MtrunA17_Chr5g0440731 57.143 77 27 4 121 196 102 173 5.15e-17 78.6
Msa0369070 MtrunA17_Chr3g0104071 35.583 163 85 4 49 200 63 216 6.45e-17 78.6
Msa0369070 MtrunA17_Chr5g0409841 36.879 141 78 4 67 203 32 165 1.83e-16 75.9
Msa0369070 MtrunA17_Chr7g0241761 40.708 113 53 3 88 200 111 209 6.73e-16 75.5
Msa0369070 MtrunA17_Chr5g0440741 58.904 73 24 4 125 196 101 168 8.99e-16 75.1
Msa0369070 MtrunA17_Chr5g0409831 43.023 86 43 2 90 169 37 122 1.24e-15 73.6
Msa0369070 MtrunA17_Chr3g0135671 38.462 117 56 2 79 195 58 158 6.18e-15 71.6
Msa0369070 MtrunA17_Chr1g0210471 29.240 171 94 4 29 199 49 192 4.20e-14 69.7
Msa0369070 MtrunA17_Chr4g0059701 33.333 117 66 2 53 169 25 129 1.22e-13 68.9
Msa0369070 MtrunA17_Chr1g0162081 45.570 79 36 2 121 199 119 190 9.25e-13 66.6
Msa0369070 MtrunA17_Chr6g0488471 37.692 130 57 6 112 241 87 192 1.27e-12 65.9
Msa0369070 MtrunA17_Chr2g0331031 39.759 83 43 1 115 197 100 175 1.51e-12 66.2
Msa0369070 MtrunA17_Chr4g0055931 36.607 112 52 2 88 199 71 163 2.93e-12 65.5
Msa0369070 MtrunA17_Chr4g0062741 44.304 79 37 2 121 199 135 206 4.14e-12 64.7
Msa0369070 MtrunA17_Chr5g0415191 55.102 49 22 0 121 169 99 147 4.92e-12 64.7
Msa0369070 MtrunA17_Chr8g0365011 35.948 153 64 7 61 199 42 174 7.91e-12 63.5
Msa0369070 MtrunA17_Chr5g0432921 45.833 96 43 5 93 187 85 172 7.92e-12 63.2
Msa0369070 MtrunA17_Chr8g0389241 44.000 75 34 1 121 195 102 168 1.14e-11 63.2
Msa0369070 MtrunA17_Chr6g0452791 56.522 46 20 0 124 169 80 125 7.74e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0369070 AT5G05790.2 41.406 128 63 3 78 201 82 201 5.90e-20 86.7
Msa0369070 AT5G05790.1 41.406 128 63 3 78 201 82 201 5.90e-20 86.7
Msa0369070 AT3G11280.2 35.870 184 90 9 25 201 35 197 4.35e-19 84.0
Msa0369070 AT3G11280.1 35.870 184 90 9 25 201 35 197 4.35e-19 84.0
Msa0369070 AT5G01200.1 45.299 117 50 4 85 200 112 215 2.68e-18 82.0
Msa0369070 AT2G38090.1 37.069 116 63 2 88 200 102 210 2.18e-16 77.0
Msa0369070 AT5G58900.1 44.681 94 45 1 107 200 124 210 4.24e-16 76.3
Msa0369070 AT5G08520.1 36.207 174 84 9 41 198 23 185 1.52e-15 74.7
Msa0369070 AT4G09450.1 39.837 123 61 4 99 215 66 181 4.18e-14 69.3
Msa0369070 AT5G04760.1 49.367 79 33 2 121 199 96 167 1.43e-13 68.2
Msa0369070 AT1G19000.2 50.877 57 28 0 113 169 91 147 5.05e-13 67.4
Msa0369070 AT1G19000.1 50.877 57 28 0 113 169 91 147 5.05e-13 67.4
Msa0369070 AT5G47390.1 35.878 131 66 3 115 245 87 199 6.92e-13 67.8
Msa0369070 AT1G70000.1 41.096 73 41 1 99 169 71 143 1.37e-12 65.9
Msa0369070 AT1G70000.2 41.096 73 41 1 99 169 71 143 1.37e-12 65.9
Msa0369070 AT3G10580.1 46.835 79 33 1 121 199 92 161 2.55e-12 65.5
Msa0369070 AT3G10580.2 46.835 79 33 1 121 199 92 161 2.58e-12 65.1
Msa0369070 AT1G49010.1 45.570 79 36 2 121 199 131 202 3.78e-12 65.5
Msa0369070 AT5G56840.1 52.632 57 27 0 121 177 88 144 2.31e-11 62.4
Msa0369070 AT5G61620.1 45.570 79 36 1 121 199 105 176 3.69e-11 62.4
Msa0369070 AT1G74840.2 55.102 49 22 0 121 169 93 141 5.95e-11 61.2
Msa0369070 AT1G74840.1 55.102 49 22 0 121 169 93 141 6.49e-11 61.2
Msa0369070 AT3G10590.1 41.667 108 50 4 114 221 101 195 8.70e-11 60.1

Find 36 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGATCTTGATCACATTCT+TGG 0.190525 3_1:+66193621 None:intergenic
TCAACCAATAATCATCTTCT+TGG 0.216049 3_1:+66194288 None:intergenic
ATTTCCAAGAAGATGATTAT+TGG 0.238910 3_1:-66194292 Msa0369070:CDS
GACTCATATGCGTGGTTATT+TGG 0.243758 3_1:-66193704 Msa0369070:CDS
ATAACTACTCTTGATCTTCT+TGG 0.251399 3_1:-66193734 Msa0369070:CDS
AGTTCTATTGTTATTATTAT+CGG 0.281602 3_1:+66194121 None:intergenic
TAGAATGGTAGAGAATATTC+TGG 0.354560 3_1:-66194342 Msa0369070:CDS
TTTGAGCATGGCTTGCTACT+TGG 0.356659 3_1:+66193837 None:intergenic
AAAATATCAAAAGGAAACTT+AGG 0.373972 3_1:-66193772 Msa0369070:CDS
CATTCAAGAAAGTGGTTCAT+TGG 0.387227 3_1:-66194070 Msa0369070:CDS
TTGAGCATGGCTTGCTACTT+GGG 0.417939 3_1:+66193838 None:intergenic
TATTTGTAGGCGTTTCTTGA+AGG 0.450413 3_1:-66193930 Msa0369070:intron
TCAAACTCAAATAATAGTTA+TGG 0.460594 3_1:-66194395 Msa0369070:CDS
AAATATCAAAAGGAAACTTA+GGG 0.461487 3_1:-66193771 Msa0369070:CDS
AGATGATTATTGGTTGATGA+AGG 0.467158 3_1:-66194282 Msa0369070:CDS
AAAGTGGAAAGAAATCTCTA+AGG 0.476209 3_1:-66193885 Msa0369070:CDS
AAAGAAATCATTCAAGAAAG+TGG 0.476326 3_1:-66194078 Msa0369070:CDS
ATTTGTAGGCGTTTCTTGAA+GGG 0.494586 3_1:-66193929 Msa0369070:intron
GATCAAGAGTAGTTATATCA+TGG 0.499404 3_1:+66193741 None:intergenic
GCATGGCTTGCTACTTGGGA+TGG 0.505418 3_1:+66193842 None:intergenic
GGACTTCTACACGGCAAAGA+AGG 0.509282 3_1:-66193908 Msa0369070:CDS
TGTTGTCGTCTTCAATCACT+TGG 0.511632 3_1:+66193678 None:intergenic
CATCGAAGAAAGTAGTAGAA+TGG 0.523965 3_1:-66194357 Msa0369070:CDS
AGAATGGTAGAGAATATTCT+GGG 0.545240 3_1:-66194341 Msa0369070:CDS
TACACGGCAAAGAAGGAAAG+TGG 0.551960 3_1:-66193901 Msa0369070:CDS
TTGATCAACACTACATAGCA+TGG 0.559638 3_1:+66194256 None:intergenic
ACTCAGCATTAACAATGGAA+TGG 0.588865 3_1:-66194439 None:intergenic
ATACATATGAACATATCAAG+TGG 0.590413 3_1:+66193586 None:intergenic
TTCTTGAAGGGACTTCTACA+CGG 0.594579 3_1:-66193917 Msa0369070:CDS
GAAGACGACAACAATAACAA+TGG 0.605151 3_1:-66193668 Msa0369070:CDS
TCAGCACAACATCGATGAAG+TGG 0.609150 3_1:-66194228 Msa0369070:CDS
CAATAGAACTACGAATATCG+TGG 0.625780 3_1:-66194108 Msa0369070:CDS
TTATTATTATCGGTTGCATG+AGG 0.632701 3_1:+66194131 None:intergenic
GCACAACATCGATGAAGTGG+TGG 0.668571 3_1:-66194225 Msa0369070:CDS
AATATCAAAAGGAAACTTAG+GGG 0.670754 3_1:-66193770 Msa0369070:CDS
GAAGTGATGACTCATATGCG+TGG 0.764866 3_1:-66193712 Msa0369070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTCTATTGTTATTATTAT+CGG + chr3_1:66193908-66193927 None:intergenic 15.0%
!!! AGTTCTATTGTTATTATTAT+CGG + chr3_1:66193908-66193927 None:intergenic 15.0%
!! TCAAACTCAAATAATAGTTA+TGG - chr3_1:66193631-66193650 Msa0369070:CDS 20.0%
!!! TTTCTTGAATGATTTCTTTT+TGG + chr3_1:66193947-66193966 None:intergenic 20.0%
!! ATGATAGCAAAAATATCAAA+AGG - chr3_1:66194245-66194264 Msa0369070:CDS 20.0%
!! AAAATATCAAAAGGAAACTT+AGG - chr3_1:66194254-66194273 Msa0369070:CDS 20.0%
!! AAATATCAAAAGGAAACTTA+GGG - chr3_1:66194255-66194274 Msa0369070:CDS 20.0%
!! TCAAACTCAAATAATAGTTA+TGG - chr3_1:66193631-66193650 Msa0369070:CDS 20.0%
!!! TTTCTTGAATGATTTCTTTT+TGG + chr3_1:66193947-66193966 None:intergenic 20.0%
!! ATGATAGCAAAAATATCAAA+AGG - chr3_1:66194245-66194264 Msa0369070:CDS 20.0%
!! AAAATATCAAAAGGAAACTT+AGG - chr3_1:66194254-66194273 Msa0369070:CDS 20.0%
!! AAATATCAAAAGGAAACTTA+GGG - chr3_1:66194255-66194274 Msa0369070:CDS 20.0%
! ATTTCCAAGAAGATGATTAT+TGG - chr3_1:66193734-66193753 Msa0369070:CDS 25.0%
! AAAGAAATCATTCAAGAAAG+TGG - chr3_1:66193948-66193967 Msa0369070:intron 25.0%
! GAAACAATAAACGATGAAAA+AGG + chr3_1:66194005-66194024 None:intergenic 25.0%
!!! GACATGGATTTTTTATTTGT+AGG - chr3_1:66194083-66194102 Msa0369070:CDS 25.0%
!!! TTTTTTCATACTTTTGAGCA+TGG + chr3_1:66194204-66194223 None:intergenic 25.0%
!! ATGCTCAAAAGTATGAAAAA+AGG - chr3_1:66194203-66194222 Msa0369070:CDS 25.0%
! AATATCAAAAGGAAACTTAG+GGG - chr3_1:66194256-66194275 Msa0369070:CDS 25.0%
! ATTTCCAAGAAGATGATTAT+TGG - chr3_1:66193734-66193753 Msa0369070:CDS 25.0%
! AAAGAAATCATTCAAGAAAG+TGG - chr3_1:66193948-66193967 Msa0369070:intron 25.0%
! GAAACAATAAACGATGAAAA+AGG + chr3_1:66194005-66194024 None:intergenic 25.0%
!!! GACATGGATTTTTTATTTGT+AGG - chr3_1:66194083-66194102 Msa0369070:CDS 25.0%
!!! TTTTTTCATACTTTTGAGCA+TGG + chr3_1:66194204-66194223 None:intergenic 25.0%
!! ATGCTCAAAAGTATGAAAAA+AGG - chr3_1:66194203-66194222 Msa0369070:CDS 25.0%
! AATATCAAAAGGAAACTTAG+GGG - chr3_1:66194256-66194275 Msa0369070:CDS 25.0%
TAGAATGGTAGAGAATATTC+TGG - chr3_1:66193684-66193703 Msa0369070:CDS 30.0%
AGAATGGTAGAGAATATTCT+GGG - chr3_1:66193685-66193704 Msa0369070:CDS 30.0%
TCAACCAATAATCATCTTCT+TGG + chr3_1:66193741-66193760 None:intergenic 30.0%
!! AGATGATTATTGGTTGATGA+AGG - chr3_1:66193744-66193763 Msa0369070:CDS 30.0%
! TTATTATTATCGGTTGCATG+AGG + chr3_1:66193898-66193917 None:intergenic 30.0%
AAAGTGGAAAGAAATCTCTA+AGG - chr3_1:66194141-66194160 Msa0369070:CDS 30.0%
GATCAAGAGTAGTTATATCA+TGG + chr3_1:66194288-66194307 None:intergenic 30.0%
ATAACTACTCTTGATCTTCT+TGG - chr3_1:66194292-66194311 Msa0369070:CDS 30.0%
TAGAATGGTAGAGAATATTC+TGG - chr3_1:66193684-66193703 Msa0369070:CDS 30.0%
AGAATGGTAGAGAATATTCT+GGG - chr3_1:66193685-66193704 Msa0369070:CDS 30.0%
TCAACCAATAATCATCTTCT+TGG + chr3_1:66193741-66193760 None:intergenic 30.0%
!! AGATGATTATTGGTTGATGA+AGG - chr3_1:66193744-66193763 Msa0369070:CDS 30.0%
! TTATTATTATCGGTTGCATG+AGG + chr3_1:66193898-66193917 None:intergenic 30.0%
AAAGTGGAAAGAAATCTCTA+AGG - chr3_1:66194141-66194160 Msa0369070:CDS 30.0%
GATCAAGAGTAGTTATATCA+TGG + chr3_1:66194288-66194307 None:intergenic 30.0%
ATAACTACTCTTGATCTTCT+TGG - chr3_1:66194292-66194311 Msa0369070:CDS 30.0%
CATCGAAGAAAGTAGTAGAA+TGG - chr3_1:66193669-66193688 Msa0369070:CDS 35.0%
TTGATCAACACTACATAGCA+TGG + chr3_1:66193773-66193792 None:intergenic 35.0%
CAATAGAACTACGAATATCG+TGG - chr3_1:66193918-66193937 Msa0369070:CDS 35.0%
CATTCAAGAAAGTGGTTCAT+TGG - chr3_1:66193956-66193975 Msa0369070:intron 35.0%
TATTTGTAGGCGTTTCTTGA+AGG - chr3_1:66194096-66194115 Msa0369070:CDS 35.0%
ATTTGTAGGCGTTTCTTGAA+GGG - chr3_1:66194097-66194116 Msa0369070:CDS 35.0%
GAAGACGACAACAATAACAA+TGG - chr3_1:66194358-66194377 Msa0369070:CDS 35.0%
CATGATCTTGATCACATTCT+TGG + chr3_1:66194408-66194427 None:intergenic 35.0%
CATCGAAGAAAGTAGTAGAA+TGG - chr3_1:66193669-66193688 Msa0369070:CDS 35.0%
TTGATCAACACTACATAGCA+TGG + chr3_1:66193773-66193792 None:intergenic 35.0%
CAATAGAACTACGAATATCG+TGG - chr3_1:66193918-66193937 Msa0369070:CDS 35.0%
CATTCAAGAAAGTGGTTCAT+TGG - chr3_1:66193956-66193975 Msa0369070:intron 35.0%
TATTTGTAGGCGTTTCTTGA+AGG - chr3_1:66194096-66194115 Msa0369070:CDS 35.0%
ATTTGTAGGCGTTTCTTGAA+GGG - chr3_1:66194097-66194116 Msa0369070:CDS 35.0%
GAAGACGACAACAATAACAA+TGG - chr3_1:66194358-66194377 Msa0369070:CDS 35.0%
CATGATCTTGATCACATTCT+TGG + chr3_1:66194408-66194427 None:intergenic 35.0%
TTCTTGAAGGGACTTCTACA+CGG - chr3_1:66194109-66194128 Msa0369070:CDS 40.0%
GACTCATATGCGTGGTTATT+TGG - chr3_1:66194322-66194341 Msa0369070:CDS 40.0%
TGTTGTCGTCTTCAATCACT+TGG + chr3_1:66194351-66194370 None:intergenic 40.0%
TTCTTGAAGGGACTTCTACA+CGG - chr3_1:66194109-66194128 Msa0369070:CDS 40.0%
GACTCATATGCGTGGTTATT+TGG - chr3_1:66194322-66194341 Msa0369070:CDS 40.0%
TGTTGTCGTCTTCAATCACT+TGG + chr3_1:66194351-66194370 None:intergenic 40.0%
TCAGCACAACATCGATGAAG+TGG - chr3_1:66193798-66193817 Msa0369070:CDS 45.0%
!! TCGACGAGCGTTTCACTTTT+TGG - chr3_1:66193841-66193860 Msa0369070:CDS 45.0%
TACACGGCAAAGAAGGAAAG+TGG - chr3_1:66194125-66194144 Msa0369070:CDS 45.0%
!! TTGAGCATGGCTTGCTACTT+GGG + chr3_1:66194191-66194210 None:intergenic 45.0%
!! TTTGAGCATGGCTTGCTACT+TGG + chr3_1:66194192-66194211 None:intergenic 45.0%
GAAGTGATGACTCATATGCG+TGG - chr3_1:66194314-66194333 Msa0369070:CDS 45.0%
TCAGCACAACATCGATGAAG+TGG - chr3_1:66193798-66193817 Msa0369070:CDS 45.0%
!! TCGACGAGCGTTTCACTTTT+TGG - chr3_1:66193841-66193860 Msa0369070:CDS 45.0%
TACACGGCAAAGAAGGAAAG+TGG - chr3_1:66194125-66194144 Msa0369070:CDS 45.0%
!! TTGAGCATGGCTTGCTACTT+GGG + chr3_1:66194191-66194210 None:intergenic 45.0%
!! TTTGAGCATGGCTTGCTACT+TGG + chr3_1:66194192-66194211 None:intergenic 45.0%
GAAGTGATGACTCATATGCG+TGG - chr3_1:66194314-66194333 Msa0369070:CDS 45.0%
GCACAACATCGATGAAGTGG+TGG - chr3_1:66193801-66193820 Msa0369070:CDS 50.0%
GCGCACACATGTGTTTGACA+TGG - chr3_1:66194067-66194086 Msa0369070:CDS 50.0%
GGACTTCTACACGGCAAAGA+AGG - chr3_1:66194118-66194137 Msa0369070:CDS 50.0%
GCACAACATCGATGAAGTGG+TGG - chr3_1:66193801-66193820 Msa0369070:CDS 50.0%
GCGCACACATGTGTTTGACA+TGG - chr3_1:66194067-66194086 Msa0369070:CDS 50.0%
GGACTTCTACACGGCAAAGA+AGG - chr3_1:66194118-66194137 Msa0369070:CDS 50.0%
!! GCATGGCTTGCTACTTGGGA+TGG + chr3_1:66194187-66194206 None:intergenic 55.0%
!! GCATGGCTTGCTACTTGGGA+TGG + chr3_1:66194187-66194206 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 66193601 66194447 66193601 ID=Msa0369070;Name=Msa0369070
chr3_1 mRNA 66193601 66194447 66193601 ID=Msa0369070-mRNA-1;Parent=Msa0369070;Name=Msa0369070-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|2|0|245
chr3_1 exon 66194053 66194447 66194053 ID=Msa0369070-mRNA-1:exon:13399;Parent=Msa0369070-mRNA-1
chr3_1 exon 66193601 66193943 66193601 ID=Msa0369070-mRNA-1:exon:13398;Parent=Msa0369070-mRNA-1
chr3_1 CDS 66194053 66194447 66194053 ID=Msa0369070-mRNA-1:cds;Parent=Msa0369070-mRNA-1
chr3_1 CDS 66193601 66193943 66193601 ID=Msa0369070-mRNA-1:cds;Parent=Msa0369070-mRNA-1
Gene Sequence

>Msa0369070

ATGGAATGGATGCATTCTTGTTTGAACTCATCAAACTCAAATAATAGTTATGGCTACATATATGACAGCATCGAAGAAAGTAGTAGAATGGTAGAGAATATTCTGGGTTACAGTCTTCATAGCATGCAGAATAATTTCCAAGAAGATGATTATTGGTTGATGAAGGAAACCATGCTATGTAGTGTTGATCAAATCAATCAGCACAACATCGATGAAGTGGTGGTAGAAAATAATATTGCATCGACGAGCGTTTCACTTTTTGGATATGATTCAGTTGATTCTTTCAACTTCTCTCCTCATGCAACCGATAATAATAACAATAGAACTACGAATATCGTGGAATCCAAAAAGAAATCATTCAAGAAAGTGGTTCATTGGTCACGTGATGAACACATGCGTTTCTTGAAGGGACTTCTACACGGCAAAGAAGGAAAGTGGAAAGAAATCTCTAAGGACTATGTAAAAACAAAGACTCCATCCCAAGTAGCAAGCCATGCTCAAAAGTATGAAAAAAGGCAAAAGCAAAGACTTGATGATGATAGCAAAAATATCAAAAGGAAACTTAGGGGTAGCATCCATGATATAACTACTCTTGATCTTCTTGGAAGTGATGACTCATATGCGTGGTTATTTGGTGACCAAGTGATTGAAGACGACAACAATAACAATGGATCACTCTCAGTCACAACTTTCAACCAAGAATGTGATCAAGATCATGAAGTTGTTGCTTCCACTTGA

Protein sequence

>Msa0369070

MEWMHSCLNSSNSNNSYGYIYDSIEESSRMVENILGYSLHSMQNNFQEDDYWLMKETMLCSVDQINQHNIDEVVVENNIASTSVSLFGYDSVDSFNFSPHATDNNNNRTTNIVESKKKSFKKVVHWSRDEHMRFLKGLLHGKEGKWKEISKDYVKTKTPSQVASHAQKYEKRQKQRLDDDSKNIKRKLRGSIHDITTLDLLGSDDSYAWLFGDQVIEDDNNNNGSLSVTTFNQECDQDHEVVAST*