AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0373100


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0373100 MtrunA17_Chr3g0122071 90.598 117 11 0 213 329 106 222 5.77e-73 225
Msa0373100 MtrunA17_Chr3g0122071 69.744 195 14 2 1 184 1 161 7.36e-54 176
Msa0373100 MtrunA17_Chr4g0064011 54.545 176 60 8 1 174 1 158 2.03e-42 147
Msa0373100 MtrunA17_Chr4g0064011 60.606 132 49 3 202 332 103 232 2.54e-39 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0373100 AT5G22990.1 51.807 83 40 0 220 302 232 314 9.74e-20 88.6
Msa0373100 AT5G22990.1 51.282 78 24 2 136 213 232 295 3.98e-16 78.6
Msa0373100 AT2G18490.1 53.425 73 33 1 207 279 142 213 5.97e-17 79.7
Msa0373100 AT2G18490.1 60.000 60 23 1 123 182 142 200 4.30e-15 74.3
Msa0373100 AT2G18490.2 53.425 73 33 1 207 279 192 263 6.70e-17 80.5
Msa0373100 AT2G18490.2 58.065 62 25 1 123 184 192 252 5.40e-15 74.7
Msa0373100 AT2G15740.1 52.564 78 32 2 209 286 219 291 1.08e-16 80.1
Msa0373100 AT2G15740.1 60.000 60 23 1 125 184 219 277 8.29e-16 77.4
Msa0373100 AT5G42640.1 52.113 71 29 2 213 279 218 287 2.98e-15 75.5
Msa0373100 AT5G42640.1 39.216 102 57 2 85 182 174 274 4.96e-13 68.9

Find 38 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATTAATATTTATATTAA+TGG 0.098823 3_1:-72078939 None:intergenic
TCAATTGGTACCAATAATTT+TGG 0.136685 3_1:+72078827 Msa0373100:CDS
AGCATGACTTCCAAAATTAT+TGG 0.299809 3_1:-72078837 None:intergenic
AGCCTACCTCACAAGAAATA+TGG 0.344617 3_1:+72079187 Msa0373100:CDS
AGCCTACCTCACAAGAAATA+TGG 0.344617 3_1:+72079539 Msa0373100:CDS
GAGATTCTTGAAGGTCAGTT+TGG 0.349001 3_1:+72078881 Msa0373100:CDS
ATTTCTTGTGAGGTAGGCTA+TGG 0.375103 3_1:-72079183 None:intergenic
ATTTCTTGTGAGGTAGGCTA+TGG 0.375103 3_1:-72079535 None:intergenic
ATCGTTCTTGTTCCGAAGCA+TGG 0.393851 3_1:-72079465 None:intergenic
ACAATAATAGATTGTTTCAA+TGG 0.400008 3_1:+72078771 Msa0373100:CDS
TATTAATATTAATTATTTGA+AGG 0.413280 3_1:-72079851 None:intergenic
AGATTCTTGAAGGTCAGTTT+GGG 0.424623 3_1:+72078882 Msa0373100:CDS
TTATCAGTACAAGCAATAAC+AGG 0.454560 3_1:-72079746 None:intergenic
ACTCATTCATCATCATCAAT+TGG 0.471297 3_1:+72078812 Msa0373100:CDS
AGCAATGAAAATATTGATGA+TGG 0.472887 3_1:+72079154 Msa0373100:CDS
AGCAATGAAAATATTGATGA+TGG 0.472887 3_1:+72079506 Msa0373100:CDS
ATTGCTCTTACCCCATGCTT+CGG 0.472890 3_1:+72079453 Msa0373100:CDS
ACATCTCCAACATCAGCAAC+AGG 0.474279 3_1:-72079100 None:intergenic
TCGTTCTTGTTCCGAAGCAT+GGG 0.482038 3_1:-72079464 None:intergenic
CAGATTCAAAAGCTCAATGA+TGG 0.486601 3_1:+72079707 Msa0373100:CDS
AATATTCCTGTTGCTGATGT+TGG 0.486932 3_1:+72079094 Msa0373100:CDS
TCTCATCTTGATCTCCGTGT+AGG 0.496262 3_1:+72079031 Msa0373100:CDS
AAAGCGATGGAGATTCTTGA+AGG 0.521307 3_1:+72078872 Msa0373100:CDS
CTCTTACCCCTCTTCCAAGT+GGG 0.526442 3_1:+72079810 Msa0373100:CDS
TTTCTTGTGAGGTAGGCTAT+GGG 0.526908 3_1:-72079182 None:intergenic
TTTCTTGTGAGGTAGGCTAT+GGG 0.526908 3_1:-72079534 None:intergenic
AGATTGTTTCAATGGATGCA+TGG 0.544477 3_1:+72078779 Msa0373100:CDS
GGAATATTAATCATTTGAGA+AGG 0.562733 3_1:-72079079 None:intergenic
TTCGGAACAAGAACGATATG+AGG 0.569024 3_1:+72079471 Msa0373100:CDS
TACGGTCCATATTTCTTGTG+AGG 0.577081 3_1:-72079193 None:intergenic
TACGGTCCATATTTCTTGTG+AGG 0.577081 3_1:-72079545 None:intergenic
GCTCTTACCCCTCTTCCAAG+TGG 0.581741 3_1:+72079809 Msa0373100:CDS
GTCCATATTTCTTGTGAGGT+AGG 0.582227 3_1:-72079189 None:intergenic
GTCCATATTTCTTGTGAGGT+AGG 0.582227 3_1:-72079541 None:intergenic
TCCGGGAATCCTAAAAGCGA+TGG 0.625731 3_1:+72078859 Msa0373100:CDS
CGTTCTTGTTCCGAAGCATG+GGG 0.647153 3_1:-72079463 None:intergenic
ATGTTAACAAGAACGATGTG+AGG 0.658974 3_1:+72079119 Msa0373100:CDS
ATTGTGATGATAAGCCTACA+CGG 0.769789 3_1:-72079045 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATAATAGATTGTTTCAA+TGG + chr3_1:72078771-72078790 Msa0373100:CDS 20.0%
!!! TTTTGTTACATTTTGAACAT+GGG - chr3_1:72079571-72079590 None:intergenic 20.0%
!!! ATTTTGTTACATTTTGAACA+TGG - chr3_1:72079572-72079591 None:intergenic 20.0%
!! ACAATAATAGATTGTTTCAA+TGG + chr3_1:72078771-72078790 Msa0373100:CDS 20.0%
!!! TTTTGTTACATTTTGAACAT+GGG - chr3_1:72079571-72079590 None:intergenic 20.0%
!!! ATTTTGTTACATTTTGAACA+TGG - chr3_1:72079572-72079591 None:intergenic 20.0%
!! TCAATTGGTACCAATAATTT+TGG + chr3_1:72078827-72078846 Msa0373100:CDS 25.0%
! GGAATATTAATCATTTGAGA+AGG - chr3_1:72079082-72079101 None:intergenic 25.0%
! AGCAATGAAAATATTGATGA+TGG + chr3_1:72079154-72079173 Msa0373100:CDS 25.0%
!! TTTTGTTGCATTTTGAACAT+GGG - chr3_1:72079219-72079238 None:intergenic 25.0%
!! ATTTTGTTGCATTTTGAACA+TGG - chr3_1:72079220-72079239 None:intergenic 25.0%
! AGAAAAAGAGATACATTTCT+AGG + chr3_1:72079310-72079329 Msa0373100:CDS 25.0%
! AGCAATGAAAATATTGATGA+TGG + chr3_1:72079154-72079173 Msa0373100:CDS 25.0%
! GAAAAAAGAGATACATTTCT+AGG + chr3_1:72079663-72079682 Msa0373100:CDS 25.0%
!! TCAATTGGTACCAATAATTT+TGG + chr3_1:72078827-72078846 Msa0373100:CDS 25.0%
! GGAATATTAATCATTTGAGA+AGG - chr3_1:72079082-72079101 None:intergenic 25.0%
! AGCAATGAAAATATTGATGA+TGG + chr3_1:72079154-72079173 Msa0373100:CDS 25.0%
!! TTTTGTTGCATTTTGAACAT+GGG - chr3_1:72079219-72079238 None:intergenic 25.0%
!! ATTTTGTTGCATTTTGAACA+TGG - chr3_1:72079220-72079239 None:intergenic 25.0%
! AGAAAAAGAGATACATTTCT+AGG + chr3_1:72079310-72079329 Msa0373100:CDS 25.0%
! AGCAATGAAAATATTGATGA+TGG + chr3_1:72079154-72079173 Msa0373100:CDS 25.0%
! GAAAAAAGAGATACATTTCT+AGG + chr3_1:72079663-72079682 Msa0373100:CDS 25.0%
ACTCATTCATCATCATCAAT+TGG + chr3_1:72078812-72078831 Msa0373100:CDS 30.0%
AGCATGACTTCCAAAATTAT+TGG - chr3_1:72078840-72078859 None:intergenic 30.0%
TTATCAGTACAAGCAATAAC+AGG - chr3_1:72079396-72079415 None:intergenic 30.0%
! TTACATTTTGAACATGGGTA+CGG - chr3_1:72079566-72079585 None:intergenic 30.0%
TTATCAGTACAAGCAATAAC+AGG - chr3_1:72079396-72079415 None:intergenic 30.0%
AAGTGGGATAAAAATCAAGT+CGG + chr3_1:72079826-72079845 Msa0373100:CDS 30.0%
ACTCATTCATCATCATCAAT+TGG + chr3_1:72078812-72078831 Msa0373100:CDS 30.0%
AGCATGACTTCCAAAATTAT+TGG - chr3_1:72078840-72078859 None:intergenic 30.0%
TTATCAGTACAAGCAATAAC+AGG - chr3_1:72079396-72079415 None:intergenic 30.0%
! TTACATTTTGAACATGGGTA+CGG - chr3_1:72079566-72079585 None:intergenic 30.0%
TTATCAGTACAAGCAATAAC+AGG - chr3_1:72079396-72079415 None:intergenic 30.0%
AAGTGGGATAAAAATCAAGT+CGG + chr3_1:72079826-72079845 Msa0373100:CDS 30.0%
AGATTGTTTCAATGGATGCA+TGG + chr3_1:72078779-72078798 Msa0373100:CDS 35.0%
!! TAATTTTGGAAGTCATGCTC+CGG + chr3_1:72078841-72078860 Msa0373100:CDS 35.0%
AGATTCTTGAAGGTCAGTTT+GGG + chr3_1:72078882-72078901 Msa0373100:CDS 35.0%
ATTGTGATGATAAGCCTACA+CGG - chr3_1:72079048-72079067 None:intergenic 35.0%
AATATTCCTGTTGCTGATGT+TGG + chr3_1:72079094-72079113 Msa0373100:CDS 35.0%
ATGTTAACAAGAACGATGTG+AGG + chr3_1:72079119-72079138 Msa0373100:CDS 35.0%
! TTGCATTTTGAACATGGGTA+CGG - chr3_1:72079214-72079233 None:intergenic 35.0%
CAGATTCAAAAGCTCAATGA+CGG + chr3_1:72079354-72079373 Msa0373100:intron 35.0%
CAGATTCAAAAGCTCAATGA+TGG + chr3_1:72079354-72079373 Msa0373100:intron 35.0%
! TTTTTATCCCACTTGGAAGA+GGG - chr3_1:72079820-72079839 None:intergenic 35.0%
! ATTTTTATCCCACTTGGAAG+AGG - chr3_1:72079821-72079840 None:intergenic 35.0%
! GACTTGATTTTTATCCCACT+TGG - chr3_1:72079827-72079846 None:intergenic 35.0%
AGATTGTTTCAATGGATGCA+TGG + chr3_1:72078779-72078798 Msa0373100:CDS 35.0%
!! TAATTTTGGAAGTCATGCTC+CGG + chr3_1:72078841-72078860 Msa0373100:CDS 35.0%
AGATTCTTGAAGGTCAGTTT+GGG + chr3_1:72078882-72078901 Msa0373100:CDS 35.0%
ATTGTGATGATAAGCCTACA+CGG - chr3_1:72079048-72079067 None:intergenic 35.0%
AATATTCCTGTTGCTGATGT+TGG + chr3_1:72079094-72079113 Msa0373100:CDS 35.0%
ATGTTAACAAGAACGATGTG+AGG + chr3_1:72079119-72079138 Msa0373100:CDS 35.0%
! TTGCATTTTGAACATGGGTA+CGG - chr3_1:72079214-72079233 None:intergenic 35.0%
CAGATTCAAAAGCTCAATGA+CGG + chr3_1:72079354-72079373 Msa0373100:intron 35.0%
CAGATTCAAAAGCTCAATGA+TGG + chr3_1:72079354-72079373 Msa0373100:intron 35.0%
! TTTTTATCCCACTTGGAAGA+GGG - chr3_1:72079820-72079839 None:intergenic 35.0%
! ATTTTTATCCCACTTGGAAG+AGG - chr3_1:72079821-72079840 None:intergenic 35.0%
! GACTTGATTTTTATCCCACT+TGG - chr3_1:72079827-72079846 None:intergenic 35.0%
!! AATTTTGGAAGTCATGCTCC+GGG + chr3_1:72078842-72078861 Msa0373100:CDS 40.0%
!! CAAGAATCTCCATCGCTTTT+AGG - chr3_1:72078871-72078890 None:intergenic 40.0%
AAAGCGATGGAGATTCTTGA+AGG + chr3_1:72078872-72078891 Msa0373100:CDS 40.0%
GAGATTCTTGAAGGTCAGTT+TGG + chr3_1:72078881-72078900 Msa0373100:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG - chr3_1:72079185-72079204 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG - chr3_1:72079186-72079205 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG + chr3_1:72079187-72079206 Msa0373100:CDS 40.0%
GTCCATATTTCTTGTGAGGT+AGG - chr3_1:72079192-72079211 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG - chr3_1:72079196-72079215 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG - chr3_1:72079348-72079367 None:intergenic 40.0%
TTCGGAACAAGAACGATATG+AGG + chr3_1:72079471-72079490 Msa0373100:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG - chr3_1:72079185-72079204 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG - chr3_1:72079186-72079205 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG + chr3_1:72079187-72079206 Msa0373100:CDS 40.0%
GTCCATATTTCTTGTGAGGT+AGG - chr3_1:72079192-72079211 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG - chr3_1:72079196-72079215 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG - chr3_1:72079348-72079367 None:intergenic 40.0%
! TTTTATCCCACTTGGAAGAG+GGG - chr3_1:72079819-72079838 None:intergenic 40.0%
!! AATTTTGGAAGTCATGCTCC+GGG + chr3_1:72078842-72078861 Msa0373100:CDS 40.0%
!! CAAGAATCTCCATCGCTTTT+AGG - chr3_1:72078871-72078890 None:intergenic 40.0%
AAAGCGATGGAGATTCTTGA+AGG + chr3_1:72078872-72078891 Msa0373100:CDS 40.0%
GAGATTCTTGAAGGTCAGTT+TGG + chr3_1:72078881-72078900 Msa0373100:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG - chr3_1:72079185-72079204 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG - chr3_1:72079186-72079205 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG + chr3_1:72079187-72079206 Msa0373100:CDS 40.0%
GTCCATATTTCTTGTGAGGT+AGG - chr3_1:72079192-72079211 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG - chr3_1:72079196-72079215 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG - chr3_1:72079348-72079367 None:intergenic 40.0%
TTCGGAACAAGAACGATATG+AGG + chr3_1:72079471-72079490 Msa0373100:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG - chr3_1:72079185-72079204 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG - chr3_1:72079186-72079205 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG + chr3_1:72079187-72079206 Msa0373100:CDS 40.0%
GTCCATATTTCTTGTGAGGT+AGG - chr3_1:72079192-72079211 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG - chr3_1:72079196-72079215 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG - chr3_1:72079348-72079367 None:intergenic 40.0%
! TTTTATCCCACTTGGAAGAG+GGG - chr3_1:72079819-72079838 None:intergenic 40.0%
!!! TCCATCGCTTTTAGGATTCC+CGG - chr3_1:72078863-72078882 None:intergenic 45.0%
TCTCATCTTGATCTCCGTGT+AGG + chr3_1:72079031-72079050 Msa0373100:CDS 45.0%
ACATCTCCAACATCAGCAAC+AGG - chr3_1:72079103-72079122 None:intergenic 45.0%
ATTGCTCTTACCCCATGCTT+CGG + chr3_1:72079453-72079472 Msa0373100:CDS 45.0%
TCGTTCTTGTTCCGAAGCAT+GGG - chr3_1:72079467-72079486 None:intergenic 45.0%
ATCGTTCTTGTTCCGAAGCA+TGG - chr3_1:72079468-72079487 None:intergenic 45.0%
!!! TCCATCGCTTTTAGGATTCC+CGG - chr3_1:72078863-72078882 None:intergenic 45.0%
TCTCATCTTGATCTCCGTGT+AGG + chr3_1:72079031-72079050 Msa0373100:CDS 45.0%
ACATCTCCAACATCAGCAAC+AGG - chr3_1:72079103-72079122 None:intergenic 45.0%
ATTGCTCTTACCCCATGCTT+CGG + chr3_1:72079453-72079472 Msa0373100:CDS 45.0%
TCGTTCTTGTTCCGAAGCAT+GGG - chr3_1:72079467-72079486 None:intergenic 45.0%
ATCGTTCTTGTTCCGAAGCA+TGG - chr3_1:72079468-72079487 None:intergenic 45.0%
!! ATCATTAATATTTATATTAA+TGG - chr3_1:72078942-72078961 None:intergenic 5.0%
!! ATCATTAATATTTATATTAA+TGG - chr3_1:72078942-72078961 None:intergenic 5.0%
TCCGGGAATCCTAAAAGCGA+TGG + chr3_1:72078859-72078878 Msa0373100:CDS 50.0%
CGTTCTTGTTCCGAAGCATG+GGG - chr3_1:72079466-72079485 None:intergenic 50.0%
CTCTTACCCCTCTTCCAAGT+GGG + chr3_1:72079810-72079829 Msa0373100:CDS 50.0%
TCCGGGAATCCTAAAAGCGA+TGG + chr3_1:72078859-72078878 Msa0373100:CDS 50.0%
CGTTCTTGTTCCGAAGCATG+GGG - chr3_1:72079466-72079485 None:intergenic 50.0%
CTCTTACCCCTCTTCCAAGT+GGG + chr3_1:72079810-72079829 Msa0373100:CDS 50.0%
GCTCTTACCCCTCTTCCAAG+TGG + chr3_1:72079809-72079828 Msa0373100:CDS 55.0%
GCTCTTACCCCTCTTCCAAG+TGG + chr3_1:72079809-72079828 Msa0373100:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 72078767 72079865 72078767 ID=Msa0373100;Name=Msa0373100
chr3_1 mRNA 72078767 72079865 72078767 ID=Msa0373100-mRNA-1;Parent=Msa0373100;Name=Msa0373100-mRNA-1;_AED=0.07;_eAED=0.10;_QI=0|0|0|1|1|1|2|0|332
chr3_1 exon 72078767 72079348 72078767 ID=Msa0373100-mRNA-1:exon:15943;Parent=Msa0373100-mRNA-1
chr3_1 exon 72079449 72079865 72079449 ID=Msa0373100-mRNA-1:exon:15944;Parent=Msa0373100-mRNA-1
chr3_1 CDS 72078767 72079348 72078767 ID=Msa0373100-mRNA-1:cds;Parent=Msa0373100-mRNA-1
chr3_1 CDS 72079449 72079865 72079449 ID=Msa0373100-mRNA-1:cds;Parent=Msa0373100-mRNA-1
Gene Sequence

>Msa0373100

ATGAACAATAATAGATTGTTTCAATGGATGCATGGTTACAACACAACTCATTCATCATCATCAATTGGTACCAATAATTTTGGAAGTCATGCTCCGGGAATCCTAAAAGCGATGGAGATTCTTGAAGGTCAGTTTGGGCATTTGCATACATCAACATCATCATCGTCATCATCCATTAATATAAATATTAATGATGACATCATCATAAATAACAACAAGAACAACAACATGATGATGACGATGAATACTTCTGCTGCTGATTTTTCTCATCTTGATCTCCGTGTAGGCTTATCATCACAATCACAAGAACTGCCTTCTCAAATGATTAATATTCCTGTTGCTGATGTTGGAGATGTTAACAAGAACGATGTGAGGAAGAAGAAGAAGAGCAATGAAAATATTGATGATGGAAGAACCCATAGCCTACCTCACAAGAAATATGGACCGTACCCATGTTCAAAATGCAACAAAATATTTGAAACATCACAAAAATTTGCTAATCATGTTTCATCAAGTCACTACAAGTTCGAAAGTGAAGAAGACAGAAAAAGAGATACATTTCTAGGATTCAGAAGAGACCGCTATGATTGCTCTTACCCCATGCTTCGGAACAAGAACGATATGAGGAAGAAGAAGAAGAGCAATGAAAATATTGATGATGGAAGAACCCATAGCCTACCTCACAAGAAATATGGACCGTACCCATGTTCAAAATGTAACAAAATATTTGAAACATCACAAAAATTTGCTAATCATGTTTCATCAAGTCACTACAAGTTCGAAAGTGAAGAAGACAGAAAAAAGAGATACATTTCTAGGATTCAGAAGAGACCGCGTCTTCAGATTCAAAAGCTCAATGATGGAACAACTACTTTTGTTCCTGTTATTGCTTGTACTGATAAGTCACATGCTTCAGTCAATGATGATGATCATAGTATGATTGCTCTTACCCCTCTTCCAAGTGGGATAAAAATCAAGTCGGAGCCTTCAAATAATTAA

Protein sequence

>Msa0373100

MNNNRLFQWMHGYNTTHSSSSIGTNNFGSHAPGILKAMEILEGQFGHLHTSTSSSSSSINININDDIIINNNKNNNMMMTMNTSAADFSHLDLRVGLSSQSQELPSQMINIPVADVGDVNKNDVRKKKKSNENIDDGRTHSLPHKKYGPYPCSKCNKIFETSQKFANHVSSSHYKFESEEDRKRDTFLGFRRDRYDCSYPMLRNKNDMRKKKKSNENIDDGRTHSLPHKKYGPYPCSKCNKIFETSQKFANHVSSSHYKFESEEDRKKRYISRIQKRPRLQIQKLNDGTTTFVPVIACTDKSHASVNDDDHSMIALTPLPSGIKIKSEPSNN*