AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0389670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0389670 MtrunA17_Chr3g0092141 89.286 196 17 2 1 196 1 192 5.85e-129 360
Msa0389670 MtrunA17_Chr4g0060761 53.889 180 66 6 5 175 101 272 1.29e-55 177
Msa0389670 MtrunA17_Chr3g0127001 49.107 112 51 1 61 172 154 259 5.55e-31 114
Msa0389670 MtrunA17_Chr1g0182631 47.321 112 53 1 61 172 127 232 6.65e-29 107
Msa0389670 MtrunA17_Chr5g0406901 36.719 128 73 2 47 167 188 314 1.88e-22 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0389670 AT1G18790.1 62.500 128 41 2 48 168 112 239 7.41e-51 165
Msa0389670 AT1G74480.1 59.649 114 42 1 55 164 129 242 3.06e-43 147
Msa0389670 AT5G66990.1 50.746 134 59 2 42 170 142 273 8.75e-42 142
Msa0389670 AT5G53040.1 55.340 103 40 2 66 168 153 249 1.99e-31 115
Msa0389670 AT4G35590.2 37.313 134 72 2 61 187 192 320 1.84e-19 84.7
Msa0389670 AT4G35590.1 38.060 134 71 3 61 187 242 370 2.75e-19 84.7

Find 37 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTTGTACATTCTTTATA+AGG 0.172964 3_1:-94666841 None:intergenic
AATTATTCAATGACAGACTA+TGG 0.286299 3_1:+94666503 None:intergenic
TCTCTAGCAGCTTGAGATAT+TGG 0.323623 3_1:-94666728 None:intergenic
AAGAAAAGGTGTAGGGATCT+TGG 0.330015 3_1:+94666776 Msa0389670:CDS
TCAATGACAGACTATGGTTA+TGG 0.334072 3_1:+94666509 None:intergenic
GCTCATGGTTATGAGGTTAA+TGG 0.347444 3_1:+94667578 Msa0389670:CDS
TACTTGTACATTCTTTATAA+GGG 0.359802 3_1:-94666840 None:intergenic
AGTTGATGCTGGATATTATT+CGG 0.383233 3_1:+94666565 Msa0389670:CDS
GGCTTACACATTTGAAGAAA+AGG 0.415724 3_1:+94666762 Msa0389670:CDS
CAATGACAGACTATGGTTAT+GGG 0.417103 3_1:+94666510 None:intergenic
CATTCTTTATAAGGGTTTGA+AGG 0.417292 3_1:-94666832 None:intergenic
TATGAGGTTAATGGAACAAC+AGG 0.443913 3_1:+94667587 Msa0389670:CDS
CTGCTAGAGACCTTAATGTT+GGG 0.489436 3_1:+94666741 Msa0389670:CDS
GAAAGAAATGAAGAAGGTAG+AGG 0.489935 3_1:+94667476 Msa0389670:CDS
ATGAATGTGATGAGAAGATA+AGG 0.493501 3_1:+94667433 Msa0389670:CDS
GCTTAGACAAGCATGTTTCA+AGG 0.510842 3_1:+94667536 Msa0389670:CDS
ATTGCAGAAAGAAATGAAGA+AGG 0.517898 3_1:+94667470 Msa0389670:CDS
AGAGAAGGCTCATGGTTATG+AGG 0.522137 3_1:+94667571 Msa0389670:CDS
GCTGCTAGAGACCTTAATGT+TGG 0.523622 3_1:+94666740 Msa0389670:CDS
AATGTGTAAGCCCAACATTA+AGG 0.530566 3_1:-94666751 None:intergenic
CACATTTGAAGAAAAGGTGT+AGG 0.540159 3_1:+94666768 Msa0389670:CDS
GAATTCAAAGGTGGCCACAT+AGG 0.544869 3_1:+94666798 Msa0389670:CDS
ATGTGGAATGAAGTTGATGC+TGG 0.550987 3_1:+94666554 Msa0389670:CDS
TACTGGAGAAAATGTTGCAA+AGG 0.556786 3_1:+94666616 Msa0389670:CDS
AGGCTAATTACAAGAAGAGA+AGG 0.568624 3_1:+94667556 Msa0389670:CDS
AGGCTCATTAGTTTCCTATG+TGG 0.571114 3_1:-94666812 None:intergenic
TGTTGCAAAGGAAATTGTGA+AGG 0.572064 3_1:+94666628 Msa0389670:CDS
ACATTTGAAGAAAAGGTGTA+GGG 0.575896 3_1:+94666769 Msa0389670:CDS
TTATTGGTGGTGCAGGAGCA+AGG 0.586794 3_1:+94667408 Msa0389670:intron
AACTTGATGAAAACACAAAG+AGG 0.589787 3_1:+94667514 Msa0389670:CDS
TTACAAGAAGAGAAGGCTCA+TGG 0.590126 3_1:+94667563 Msa0389670:CDS
GTAGGGATCTTGGAATTCAA+AGG 0.603743 3_1:+94666786 Msa0389670:CDS
AATGACAGACTATGGTTATG+GGG 0.605489 3_1:+94666511 None:intergenic
GTTGCAAAGGAAATTGTGAA+GGG 0.628143 3_1:+94666629 Msa0389670:CDS
GCATCAACTTCATTCCACAT+TGG 0.632538 3_1:-94666551 None:intergenic
TGAGAAGATAAGGAATGCTG+TGG 0.656309 3_1:+94667443 Msa0389670:CDS
GGGATCTTGGAATTCAAAGG+TGG 0.676630 3_1:+94666789 Msa0389670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTATTTTTATTTTTTTA+CGG + chr3_1:94667269-94667288 Msa0389670:intron 0.0%
!!! TATTTATTTTTATTTTTTTA+CGG + chr3_1:94667269-94667288 Msa0389670:intron 0.0%
!!! ATTTTTTTTACTAATTTTTG+TGG + chr3_1:94667206-94667225 Msa0389670:intron 10.0%
!!! ATTTTTTTTACTAATTTTTG+TGG + chr3_1:94667206-94667225 Msa0389670:intron 10.0%
!! ATTGCAAACTAAAAAAATAT+TGG - chr3_1:94666870-94666889 None:intergenic 15.0%
!!! AGTAAGAATGAAAAATATTT+TGG - chr3_1:94667053-94667072 None:intergenic 15.0%
!!! TTGAAACGTTTTATTATTAT+TGG + chr3_1:94667392-94667411 Msa0389670:intron 15.0%
!! ATTGCAAACTAAAAAAATAT+TGG - chr3_1:94666870-94666889 None:intergenic 15.0%
!!! AGTAAGAATGAAAAATATTT+TGG - chr3_1:94667053-94667072 None:intergenic 15.0%
!!! TTGAAACGTTTTATTATTAT+TGG + chr3_1:94667392-94667411 Msa0389670:intron 15.0%
!! TACTTGTACATTCTTTATAA+GGG - chr3_1:94666843-94666862 None:intergenic 20.0%
!! TTACTTGTACATTCTTTATA+AGG - chr3_1:94666844-94666863 None:intergenic 20.0%
!!! TTCATTCTTACTTATTTTTG+TGG + chr3_1:94667061-94667080 Msa0389670:intron 20.0%
!!! AAACGTTTTATTATTATTGG+TGG + chr3_1:94667395-94667414 Msa0389670:intron 20.0%
!! TACTTGTACATTCTTTATAA+GGG - chr3_1:94666843-94666862 None:intergenic 20.0%
!! TTACTTGTACATTCTTTATA+AGG - chr3_1:94666844-94666863 None:intergenic 20.0%
!!! TTCATTCTTACTTATTTTTG+TGG + chr3_1:94667061-94667080 Msa0389670:intron 20.0%
!!! AAACGTTTTATTATTATTGG+TGG + chr3_1:94667395-94667414 Msa0389670:intron 20.0%
!! TTAGTGATGGAATTAAAGAT+CGG - chr3_1:94666976-94666995 None:intergenic 25.0%
!! AATTCCATCACTAAATTTTG+CGG + chr3_1:94666982-94667001 Msa0389670:intron 25.0%
!!! GAATGAAAAATATTTTGGCA+AGG - chr3_1:94667048-94667067 None:intergenic 25.0%
!!! TGTAGTGAATTGTTTTAATG+TGG + chr3_1:94667108-94667127 Msa0389670:intron 25.0%
!!! AATGTGGATTACTTTTTTTG+TGG + chr3_1:94667124-94667143 Msa0389670:intron 25.0%
!!! TTACTTTTTTTGTGGCTTTT+TGG + chr3_1:94667132-94667151 Msa0389670:intron 25.0%
!! TTAGTGATGGAATTAAAGAT+CGG - chr3_1:94666976-94666995 None:intergenic 25.0%
!! AATTCCATCACTAAATTTTG+CGG + chr3_1:94666982-94667001 Msa0389670:intron 25.0%
!!! GAATGAAAAATATTTTGGCA+AGG - chr3_1:94667048-94667067 None:intergenic 25.0%
!!! TGTAGTGAATTGTTTTAATG+TGG + chr3_1:94667108-94667127 Msa0389670:intron 25.0%
!!! AATGTGGATTACTTTTTTTG+TGG + chr3_1:94667124-94667143 Msa0389670:intron 25.0%
!!! TTACTTTTTTTGTGGCTTTT+TGG + chr3_1:94667132-94667151 Msa0389670:intron 25.0%
! AGTTGATGCTGGATATTATT+CGG + chr3_1:94666565-94666584 Msa0389670:CDS 30.0%
! TTTTCGATTAGCAACATTAC+TGG + chr3_1:94666599-94666618 Msa0389670:CDS 30.0%
! ACATTTGAAGAAAAGGTGTA+GGG + chr3_1:94666769-94666788 Msa0389670:CDS 30.0%
CATTCTTTATAAGGGTTTGA+AGG - chr3_1:94666835-94666854 None:intergenic 30.0%
!! TGGAATTAAAGATCGGTTTT+AGG - chr3_1:94666969-94666988 None:intergenic 30.0%
! TGCAAGCAAATCATGATTTT+AGG - chr3_1:94667174-94667193 None:intergenic 30.0%
TTGATTTAGCTAGCAAATCA+TGG - chr3_1:94667307-94667326 None:intergenic 30.0%
!! TTTATTATTATTGGTGGTGC+AGG + chr3_1:94667401-94667420 Msa0389670:intron 30.0%
ATGAATGTGATGAGAAGATA+AGG + chr3_1:94667433-94667452 Msa0389670:CDS 30.0%
ATTGCAGAAAGAAATGAAGA+AGG + chr3_1:94667470-94667489 Msa0389670:CDS 30.0%
!! TTTTCATCAAGTTGCAAATC+TGG - chr3_1:94667507-94667526 None:intergenic 30.0%
AACTTGATGAAAACACAAAG+AGG + chr3_1:94667514-94667533 Msa0389670:CDS 30.0%
! AGTTGATGCTGGATATTATT+CGG + chr3_1:94666565-94666584 Msa0389670:CDS 30.0%
! TTTTCGATTAGCAACATTAC+TGG + chr3_1:94666599-94666618 Msa0389670:CDS 30.0%
! ACATTTGAAGAAAAGGTGTA+GGG + chr3_1:94666769-94666788 Msa0389670:CDS 30.0%
CATTCTTTATAAGGGTTTGA+AGG - chr3_1:94666835-94666854 None:intergenic 30.0%
!! TGGAATTAAAGATCGGTTTT+AGG - chr3_1:94666969-94666988 None:intergenic 30.0%
! TGCAAGCAAATCATGATTTT+AGG - chr3_1:94667174-94667193 None:intergenic 30.0%
TTGATTTAGCTAGCAAATCA+TGG - chr3_1:94667307-94667326 None:intergenic 30.0%
!! TTTATTATTATTGGTGGTGC+AGG + chr3_1:94667401-94667420 Msa0389670:intron 30.0%
ATGAATGTGATGAGAAGATA+AGG + chr3_1:94667433-94667452 Msa0389670:CDS 30.0%
ATTGCAGAAAGAAATGAAGA+AGG + chr3_1:94667470-94667489 Msa0389670:CDS 30.0%
!! TTTTCATCAAGTTGCAAATC+TGG - chr3_1:94667507-94667526 None:intergenic 30.0%
AACTTGATGAAAACACAAAG+AGG + chr3_1:94667514-94667533 Msa0389670:CDS 30.0%
TTATGGGGATGCTAAAAATG+TGG + chr3_1:94666526-94666545 Msa0389670:CDS 35.0%
CTAAAAATGTGGTTCCAATG+TGG + chr3_1:94666537-94666556 Msa0389670:CDS 35.0%
TACTGGAGAAAATGTTGCAA+AGG + chr3_1:94666616-94666635 Msa0389670:CDS 35.0%
TGTTGCAAAGGAAATTGTGA+AGG + chr3_1:94666628-94666647 Msa0389670:CDS 35.0%
GTTGCAAAGGAAATTGTGAA+GGG + chr3_1:94666629-94666648 Msa0389670:CDS 35.0%
AATGTGTAAGCCCAACATTA+AGG - chr3_1:94666754-94666773 None:intergenic 35.0%
GGCTTACACATTTGAAGAAA+AGG + chr3_1:94666762-94666781 Msa0389670:CDS 35.0%
! CACATTTGAAGAAAAGGTGT+AGG + chr3_1:94666768-94666787 Msa0389670:CDS 35.0%
CATAATCACATTGTGTAGTG+AGG + chr3_1:94666923-94666942 Msa0389670:intron 35.0%
! GGCAAGGAAATCATGATTTT+AGG - chr3_1:94667032-94667051 None:intergenic 35.0%
!! TTTTGTGGCTTTTTGGTAAG+CGG + chr3_1:94667139-94667158 Msa0389670:intron 35.0%
GAAAGAAATGAAGAAGGTAG+AGG + chr3_1:94667476-94667495 Msa0389670:CDS 35.0%
AGGCTAATTACAAGAAGAGA+AGG + chr3_1:94667556-94667575 Msa0389670:CDS 35.0%
TATGAGGTTAATGGAACAAC+AGG + chr3_1:94667587-94667606 Msa0389670:CDS 35.0%
TTATGGGGATGCTAAAAATG+TGG + chr3_1:94666526-94666545 Msa0389670:CDS 35.0%
CTAAAAATGTGGTTCCAATG+TGG + chr3_1:94666537-94666556 Msa0389670:CDS 35.0%
TACTGGAGAAAATGTTGCAA+AGG + chr3_1:94666616-94666635 Msa0389670:CDS 35.0%
TGTTGCAAAGGAAATTGTGA+AGG + chr3_1:94666628-94666647 Msa0389670:CDS 35.0%
GTTGCAAAGGAAATTGTGAA+GGG + chr3_1:94666629-94666648 Msa0389670:CDS 35.0%
AATGTGTAAGCCCAACATTA+AGG - chr3_1:94666754-94666773 None:intergenic 35.0%
GGCTTACACATTTGAAGAAA+AGG + chr3_1:94666762-94666781 Msa0389670:CDS 35.0%
! CACATTTGAAGAAAAGGTGT+AGG + chr3_1:94666768-94666787 Msa0389670:CDS 35.0%
CATAATCACATTGTGTAGTG+AGG + chr3_1:94666923-94666942 Msa0389670:intron 35.0%
! GGCAAGGAAATCATGATTTT+AGG - chr3_1:94667032-94667051 None:intergenic 35.0%
!! TTTTGTGGCTTTTTGGTAAG+CGG + chr3_1:94667139-94667158 Msa0389670:intron 35.0%
GAAAGAAATGAAGAAGGTAG+AGG + chr3_1:94667476-94667495 Msa0389670:CDS 35.0%
AGGCTAATTACAAGAAGAGA+AGG + chr3_1:94667556-94667575 Msa0389670:CDS 35.0%
TATGAGGTTAATGGAACAAC+AGG + chr3_1:94667587-94667606 Msa0389670:CDS 35.0%
GCATCAACTTCATTCCACAT+TGG - chr3_1:94666554-94666573 None:intergenic 40.0%
!! ATGTGGAATGAAGTTGATGC+TGG + chr3_1:94666554-94666573 Msa0389670:CDS 40.0%
TCTCTAGCAGCTTGAGATAT+TGG - chr3_1:94666731-94666750 None:intergenic 40.0%
CTGCTAGAGACCTTAATGTT+GGG + chr3_1:94666741-94666760 Msa0389670:CDS 40.0%
! AAGAAAAGGTGTAGGGATCT+TGG + chr3_1:94666776-94666795 Msa0389670:CDS 40.0%
GTAGGGATCTTGGAATTCAA+AGG + chr3_1:94666786-94666805 Msa0389670:CDS 40.0%
! AGGCTCATTAGTTTCCTATG+TGG - chr3_1:94666815-94666834 None:intergenic 40.0%
CAAGCCGCAAAATTTAGTGA+TGG - chr3_1:94666989-94667008 None:intergenic 40.0%
! CATCACTAAATTTTGCGGCT+TGG + chr3_1:94666987-94667006 Msa0389670:intron 40.0%
TGAGAAGATAAGGAATGCTG+TGG + chr3_1:94667443-94667462 Msa0389670:CDS 40.0%
GCTTAGACAAGCATGTTTCA+AGG + chr3_1:94667536-94667555 Msa0389670:CDS 40.0%
TTACAAGAAGAGAAGGCTCA+TGG + chr3_1:94667563-94667582 Msa0389670:CDS 40.0%
GCTCATGGTTATGAGGTTAA+TGG + chr3_1:94667578-94667597 Msa0389670:CDS 40.0%
GCATCAACTTCATTCCACAT+TGG - chr3_1:94666554-94666573 None:intergenic 40.0%
!! ATGTGGAATGAAGTTGATGC+TGG + chr3_1:94666554-94666573 Msa0389670:CDS 40.0%
TCTCTAGCAGCTTGAGATAT+TGG - chr3_1:94666731-94666750 None:intergenic 40.0%
CTGCTAGAGACCTTAATGTT+GGG + chr3_1:94666741-94666760 Msa0389670:CDS 40.0%
! AAGAAAAGGTGTAGGGATCT+TGG + chr3_1:94666776-94666795 Msa0389670:CDS 40.0%
GTAGGGATCTTGGAATTCAA+AGG + chr3_1:94666786-94666805 Msa0389670:CDS 40.0%
! AGGCTCATTAGTTTCCTATG+TGG - chr3_1:94666815-94666834 None:intergenic 40.0%
CAAGCCGCAAAATTTAGTGA+TGG - chr3_1:94666989-94667008 None:intergenic 40.0%
! CATCACTAAATTTTGCGGCT+TGG + chr3_1:94666987-94667006 Msa0389670:intron 40.0%
TGAGAAGATAAGGAATGCTG+TGG + chr3_1:94667443-94667462 Msa0389670:CDS 40.0%
GCTTAGACAAGCATGTTTCA+AGG + chr3_1:94667536-94667555 Msa0389670:CDS 40.0%
TTACAAGAAGAGAAGGCTCA+TGG + chr3_1:94667563-94667582 Msa0389670:CDS 40.0%
GCTCATGGTTATGAGGTTAA+TGG + chr3_1:94667578-94667597 Msa0389670:CDS 40.0%
GCTGCTAGAGACCTTAATGT+TGG + chr3_1:94666740-94666759 Msa0389670:CDS 45.0%
GGGATCTTGGAATTCAAAGG+TGG + chr3_1:94666789-94666808 Msa0389670:CDS 45.0%
GAATTCAAAGGTGGCCACAT+AGG + chr3_1:94666798-94666817 Msa0389670:CDS 45.0%
!! CACTAAATTTTGCGGCTTGG+TGG + chr3_1:94666990-94667009 Msa0389670:intron 45.0%
AGAGAAGGCTCATGGTTATG+AGG + chr3_1:94667571-94667590 Msa0389670:CDS 45.0%
GCTGCTAGAGACCTTAATGT+TGG + chr3_1:94666740-94666759 Msa0389670:CDS 45.0%
GGGATCTTGGAATTCAAAGG+TGG + chr3_1:94666789-94666808 Msa0389670:CDS 45.0%
GAATTCAAAGGTGGCCACAT+AGG + chr3_1:94666798-94666817 Msa0389670:CDS 45.0%
!! CACTAAATTTTGCGGCTTGG+TGG + chr3_1:94666990-94667009 Msa0389670:intron 45.0%
AGAGAAGGCTCATGGTTATG+AGG + chr3_1:94667571-94667590 Msa0389670:CDS 45.0%
!! TTATTGGTGGTGCAGGAGCA+AGG + chr3_1:94667408-94667427 Msa0389670:intron 50.0%
!! TTATTGGTGGTGCAGGAGCA+AGG + chr3_1:94667408-94667427 Msa0389670:intron 50.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 94666512 94667680 94666512 ID=Msa0389670;Name=Msa0389670
chr3_1 mRNA 94666512 94667680 94666512 ID=Msa0389670-mRNA-1;Parent=Msa0389670;Name=Msa0389670-mRNA-1;_AED=0.04;_eAED=0.06;_QI=0|0|0|1|1|1|2|0|201
chr3_1 exon 94666512 94666859 94666512 ID=Msa0389670-mRNA-1:exon:26912;Parent=Msa0389670-mRNA-1
chr3_1 exon 94667423 94667680 94667423 ID=Msa0389670-mRNA-1:exon:26913;Parent=Msa0389670-mRNA-1
chr3_1 CDS 94666512 94666859 94666512 ID=Msa0389670-mRNA-1:cds;Parent=Msa0389670-mRNA-1
chr3_1 CDS 94667423 94667680 94667423 ID=Msa0389670-mRNA-1:cds;Parent=Msa0389670-mRNA-1
Gene Sequence

>Msa0389670

ATGACAGACTATGGTTATGGGGATGCTAAAAATGTGGTTCCAATGTGGAATGAAGTTGATGCTGGATATTATTCGGATAAACATGTCTTTTCGATTAGCAACATTACTGGAGAAAATGTTGCAAAGGAAATTGTGAAGGGTAAGAGATGTAGAGAAGAGAATACTAATAGTGCAAGAATGCTAACAAAAGAAACAATTTCTGAGTACTTCTATGTGCCAATATCTCAAGCTGCTAGAGACCTTAATGTTGGGCTTACACATTTGAAGAAAAGGTGTAGGGATCTTGGAATTCAAAGGTGGCCACATAGGAAACTAATGAGCCTTCAAACCCTTATAAAGAATGTACAAGAGCAAGGTAATGAATGTGATGAGAAGATAAGGAATGCTGTGGAAGTATTGCAGAAAGAAATGAAGAAGGTAGAGGAAAAGCCAGATTTGCAACTTGATGAAAACACAAAGAGGCTTAGACAAGCATGTTTCAAGGCTAATTACAAGAAGAGAAGGCTCATGGTTATGAGGTTAATGGAACAACAGGAGTCTTCTCTCAGTGAAAGTGCAAGTGTTGATGATCACAAAAAGTCAAATTTCTTCTTAACATCATGTTAA

Protein sequence

>Msa0389670

MTDYGYGDAKNVVPMWNEVDAGYYSDKHVFSISNITGENVAKEIVKGKRCREENTNSARMLTKETISEYFYVPISQAARDLNVGLTHLKKRCRDLGIQRWPHRKLMSLQTLIKNVQEQGNECDEKIRNAVEVLQKEMKKVEEKPDLQLDENTKRLRQACFKANYKKRRLMVMRLMEQQESSLSESASVDDHKKSNFFLTSC*