AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0391170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0391170 MtrunA17_Chr8g0373361 89.236 288 18 2 2 289 136 410 0.0 512
Msa0391170 MtrunA17_Chr8g0373001 64.111 287 87 6 2 284 131 405 1.45e-116 341
Msa0391170 MtrunA17_Chr5g0404321 53.793 290 114 8 2 284 133 409 1.50e-89 272
Msa0391170 MtrunA17_Chr8g0373331 46.918 292 77 9 2 286 130 350 5.21e-65 207
Msa0391170 MtrunA17_Chr8g0373351 50.877 228 68 5 66 283 93 286 2.65e-62 198
Msa0391170 MtrunA17_Chr1g0155301 43.253 289 135 10 2 283 148 414 3.51e-61 199
Msa0391170 MtrunA17_Chr2g0286441 39.384 292 144 12 2 283 117 385 3.13e-51 173
Msa0391170 MtrunA17_Chr1g0196071 39.189 296 139 14 2 284 128 395 3.12e-44 154
Msa0391170 MtrunA17_Chr1g0201891 38.535 314 142 14 2 284 131 424 2.95e-43 152
Msa0391170 MtrunA17_Chr2g0278511 38.978 313 137 15 2 284 132 420 3.59e-43 152
Msa0391170 MtrunA17_Chr2g0315641 38.328 287 131 9 2 284 120 364 2.92e-42 149
Msa0391170 MtrunA17_Chr2g0315651 34.828 290 140 10 1 284 114 360 3.77e-34 127
Msa0391170 MtrunA17_Chr8g0373341 48.819 127 58 4 158 281 198 320 1.58e-23 97.8
Msa0391170 MtrunA17_Chr8g0373341 62.222 90 23 3 2 82 110 197 1.97e-23 97.8
Msa0391170 MtrunA17_Chr1g0156151 44.898 98 38 3 2 92 129 217 3.97e-16 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0391170 AT2G18280.2 40.625 288 137 12 2 283 126 385 6.90e-52 175
Msa0391170 AT2G18280.1 40.625 288 137 12 2 283 126 385 6.90e-52 175
Msa0391170 AT1G47270.3 40.690 290 127 14 5 283 125 380 2.40e-49 168
Msa0391170 AT1G47270.2 40.690 290 127 14 5 283 149 404 3.29e-49 168
Msa0391170 AT1G47270.1 40.690 290 127 14 5 283 150 405 3.58e-49 168
Msa0391170 AT2G47900.2 38.408 289 151 11 2 283 130 398 2.95e-46 160
Msa0391170 AT2G47900.1 38.408 289 151 11 2 283 130 398 2.95e-46 160
Msa0391170 AT2G47900.3 38.276 290 151 11 2 283 130 399 7.94e-46 159
Msa0391170 AT1G53320.1 37.979 287 138 7 2 284 122 372 2.67e-44 154
Msa0391170 AT1G25280.1 37.188 320 146 14 2 284 137 438 3.99e-42 150
Msa0391170 AT1G76900.2 36.446 332 144 14 2 284 135 448 6.60e-42 150
Msa0391170 AT1G76900.1 36.446 332 144 14 2 284 135 448 6.60e-42 150
Msa0391170 AT1G76900.3 36.446 332 144 14 2 284 135 448 6.60e-42 150
Msa0391170 AT1G43640.2 35.505 307 155 12 2 283 67 355 2.08e-39 141
Msa0391170 AT1G43640.1 35.505 307 155 12 2 283 133 421 5.11e-39 142
Msa0391170 AT5G18680.2 37.075 294 143 15 2 283 26 289 5.46e-39 139
Msa0391170 AT5G18680.1 37.075 294 143 15 2 283 118 381 3.98e-38 139
Msa0391170 AT3G06380.2 35.889 287 153 12 2 283 20 280 5.25e-38 136
Msa0391170 AT1G25280.3 36.691 278 125 12 40 284 1 260 5.66e-38 135
Msa0391170 AT1G25280.2 36.691 278 125 12 40 284 1 260 5.66e-38 135
Msa0391170 AT3G06380.1 35.192 287 155 12 2 283 112 372 4.81e-37 135

Find 64 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACATGCTGGAACTCTTCT+AGG 0.289252 3_2:-2684341 None:intergenic
GATTGAACTGCAGCATTATC+TGG 0.330974 3_2:-2684275 None:intergenic
TTTACCAACAAATTTGTTGC+TGG 0.348268 3_2:-2684099 None:intergenic
AGTGCTGGTGAACGAGAAAA+AGG 0.350410 3_2:-2684515 None:intergenic
CCATGGTATGAATCTTTCAT+TGG 0.357531 3_2:-2684551 None:intergenic
TAGATGCCACCACACTAATA+TGG 0.359418 3_2:-2684691 None:intergenic
ATAGAGCTTTGACTCAGTTC+TGG 0.369854 3_2:-2684590 None:intergenic
CAAGCCAGCAACAAATTTGT+TGG 0.370996 3_2:+2684095 Msa0391170:CDS
ATTCCTGTTGTACTTAGGCT+TGG 0.405477 3_2:+2683667 Msa0391170:CDS
CTGAGACGGGTATCGAGTTC+AGG 0.416521 3_2:-2684433 None:intergenic
TGATCAACCGATCCAGTGTC+GGG 0.421360 3_2:+2684652 Msa0391170:CDS
ATCTGTCTCATAATGTTTCA+CGG 0.424549 3_2:+2684744 Msa0391170:CDS
CCAGCATGTAGCCATCTTGT+TGG 0.449241 3_2:+2684353 Msa0391170:CDS
TGCTAAGGATACGACAAATT+CGG 0.458151 3_2:-2684057 None:intergenic
GCTCCAAGCCTAAGTACAAC+AGG 0.472048 3_2:-2683670 None:intergenic
CGGAGCATCGTCCCCGACAC+TGG 0.472417 3_2:-2684664 None:intergenic
AGCTAAAGAGGGTAATCTTA+CGG 0.472647 3_2:+2684771 Msa0391170:CDS
TCTACATTCCTGTTGTACTT+AGG 0.472892 3_2:+2683662 Msa0391170:CDS
AGGATGCAGTTGATCTTTCG+TGG 0.479928 3_2:-2684413 None:intergenic
AAAAGGTATATCAAATATTG+AGG 0.483208 3_2:-2684498 None:intergenic
TGTTGGCACCGTTTCCTATG+AGG 0.484236 3_2:+2684370 Msa0391170:CDS
TTTCACGGGCGGAGCTAAAG+AGG 0.499217 3_2:+2684759 Msa0391170:CDS
CCCGTCTCAGCTCTTTATGA+AGG 0.499502 3_2:+2684446 Msa0391170:CDS
AATGACAGTGATCACCTCAT+AGG 0.505003 3_2:-2684384 None:intergenic
TGTCGGGGACGATGCTCCGC+GGG 0.509673 3_2:+2684668 Msa0391170:CDS
TCAAGCAGATGGCAAAGGCT+TGG 0.517708 3_2:-2684853 None:intergenic
GCAGATGGCAAAGGCTTGGA+AGG 0.525320 3_2:-2684849 None:intergenic
AATATTGAGGGTAACGATGT+TGG 0.530649 3_2:-2684485 None:intergenic
CGCGGGCCATATTAGTGTGG+TGG 0.547601 3_2:+2684685 Msa0391170:CDS
CCGTCTCAGCTCTTTATGAA+GGG 0.557348 3_2:+2684447 Msa0391170:CDS
TTAGAATTCAATATCCCGCT+TGG 0.557985 3_2:-2684308 None:intergenic
TCTATAAAGAACTTTCAACT+TGG 0.558764 3_2:+2684710 Msa0391170:CDS
TCTGTCTCATAATGTTTCAC+GGG 0.563535 3_2:+2684745 Msa0391170:CDS
TTCATACCATGGAAGCCTAT+CGG 0.564608 3_2:+2684562 Msa0391170:CDS
CCCTTCATAAAGAGCTGAGA+CGG 0.565562 3_2:-2684447 None:intergenic
AATCAAATTGTCGACCAAGC+GGG 0.569918 3_2:+2684294 Msa0391170:CDS
AGTGATCACCTCATAGGAAA+CGG 0.575348 3_2:-2684378 None:intergenic
TTGATCAACCGATCCAGTGT+CGG 0.575709 3_2:+2684651 Msa0391170:CDS
TCAGTTCTGGTAGCTCCGAT+AGG 0.578337 3_2:-2684577 None:intergenic
CCAATGAAAGATTCATACCA+TGG 0.582725 3_2:+2684551 Msa0391170:CDS
CCTTCATAAAGAGCTGAGAC+GGG 0.585813 3_2:-2684446 None:intergenic
CCAACAAGATGGCTACATGC+TGG 0.587008 3_2:-2684353 None:intergenic
AGGAAACGGTGCCAACAAGA+TGG 0.588598 3_2:-2684364 None:intergenic
TCTCATTGACGTCAAGCAGA+TGG 0.589615 3_2:-2684864 None:intergenic
ACTGTCGTATATAGAGAACT+TGG 0.591158 3_2:-2684246 None:intergenic
GTAGCTCCGATAGGCTTCCA+TGG 0.591182 3_2:-2684568 None:intergenic
AAAGGTATATCAAATATTGA+GGG 0.595500 3_2:-2684497 None:intergenic
AGAGATGTTTATGAGTGTCA+AGG 0.596164 3_2:-2684618 None:intergenic
CATCGTCCCCGACACTGGAT+CGG 0.599982 3_2:-2684659 None:intergenic
GAGGGTAACGATGTTGGAGT+CGG 0.604172 3_2:-2684479 None:intergenic
TGACGTCAAGCAGATGGCAA+AGG 0.606195 3_2:-2684858 None:intergenic
CGTCTCAGCTCTTTATGAAG+GGG 0.613180 3_2:+2684448 Msa0391170:CDS
GAGATGTTTATGAGTGTCAA+GGG 0.619622 3_2:-2684617 None:intergenic
GTCTCATAATGTTTCACGGG+CGG 0.621282 3_2:+2684748 Msa0391170:CDS
AAGGCTTGGAAGGCAGAGAG+TGG 0.622272 3_2:-2684839 None:intergenic
GTCTCAGCTCTTTATGAAGG+GGG 0.630906 3_2:+2684449 Msa0391170:CDS
CACCACACTAATATGGCCCG+CGG 0.640194 3_2:-2684684 None:intergenic
TTCACGGGCGGAGCTAAAGA+GGG 0.644674 3_2:+2684760 Msa0391170:CDS
CAATCAAATTGTCGACCAAG+CGG 0.651811 3_2:+2684293 Msa0391170:CDS
CTCCGCGGGCCATATTAGTG+TGG 0.653740 3_2:+2684682 Msa0391170:CDS
GTGTCGGGGACGATGCTCCG+CGG 0.659179 3_2:+2684667 Msa0391170:CDS
GATCAACCGATCCAGTGTCG+GGG 0.678042 3_2:+2684653 Msa0391170:CDS
ATTATGAGACAGATGAACAG+AGG 0.698328 3_2:-2684735 None:intergenic
TGAACTGCAGCATTATCTGG+TGG 0.700796 3_2:-2684272 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTACCCTTTTATTCTAT+TGG + chr3_2:2684152-2684171 Msa0391170:intron 20.0%
!!! AAAGGTATATCAAATATTGA+GGG - chr3_2:2684500-2684519 None:intergenic 20.0%
!!! AAAAGGTATATCAAATATTG+AGG - chr3_2:2684501-2684520 None:intergenic 20.0%
!!! TATTTACCCTTTTATTCTAT+TGG + chr3_2:2684152-2684171 Msa0391170:intron 20.0%
!!! AAAGGTATATCAAATATTGA+GGG - chr3_2:2684500-2684519 None:intergenic 20.0%
!!! AAAAGGTATATCAAATATTG+AGG - chr3_2:2684501-2684520 None:intergenic 20.0%
!! ACTTACTTGCTCTAAATTTA+TGG + chr3_2:2683817-2683836 Msa0391170:intron 25.0%
!! CTTACTTGCTCTAAATTTAT+GGG + chr3_2:2683818-2683837 Msa0391170:intron 25.0%
! ATTCATTGTCATTATGATTG+TGG + chr3_2:2683865-2683884 Msa0391170:intron 25.0%
!!! ACAAATTTGTTGGTAAAGTA+AGG + chr3_2:2684105-2684124 Msa0391170:intron 25.0%
! GAAAAGCCAATAGAATAAAA+GGG - chr3_2:2684161-2684180 None:intergenic 25.0%
! TGAAAAGCCAATAGAATAAA+AGG - chr3_2:2684162-2684181 None:intergenic 25.0%
!! TTGATTTTTATACATATGCC+AGG + chr3_2:2684207-2684226 Msa0391170:CDS 25.0%
! TCTATAAAGAACTTTCAACT+TGG + chr3_2:2684710-2684729 Msa0391170:CDS 25.0%
! TGAAAAAGATCTATTCATCA+TGG + chr3_2:2684805-2684824 Msa0391170:CDS 25.0%
!! ACTTACTTGCTCTAAATTTA+TGG + chr3_2:2683817-2683836 Msa0391170:intron 25.0%
!! CTTACTTGCTCTAAATTTAT+GGG + chr3_2:2683818-2683837 Msa0391170:intron 25.0%
! ATTCATTGTCATTATGATTG+TGG + chr3_2:2683865-2683884 Msa0391170:intron 25.0%
!!! ACAAATTTGTTGGTAAAGTA+AGG + chr3_2:2684105-2684124 Msa0391170:intron 25.0%
! GAAAAGCCAATAGAATAAAA+GGG - chr3_2:2684161-2684180 None:intergenic 25.0%
! TGAAAAGCCAATAGAATAAA+AGG - chr3_2:2684162-2684181 None:intergenic 25.0%
!! TTGATTTTTATACATATGCC+AGG + chr3_2:2684207-2684226 Msa0391170:CDS 25.0%
! TCTATAAAGAACTTTCAACT+TGG + chr3_2:2684710-2684729 Msa0391170:CDS 25.0%
! TGAAAAAGATCTATTCATCA+TGG + chr3_2:2684805-2684824 Msa0391170:CDS 25.0%
TCAGCAATGAAATACTTACA+TGG - chr3_2:2683695-2683714 None:intergenic 30.0%
CATTGTCTTAGAAAAAGAGA+TGG - chr3_2:2683724-2683743 None:intergenic 30.0%
!!! AAGCCAGATTGTTTTTGTTT+TGG + chr3_2:2683969-2683988 Msa0391170:intron 30.0%
CAACCAAAACAAAAACAATC+TGG - chr3_2:2683975-2683994 None:intergenic 30.0%
!! TTTACCAACAAATTTGTTGC+TGG - chr3_2:2684102-2684121 None:intergenic 30.0%
! TCAAGTTGTGAAGACTATAA+GGG - chr3_2:2684191-2684210 None:intergenic 30.0%
! ATCAAGTTGTGAAGACTATA+AGG - chr3_2:2684192-2684211 None:intergenic 30.0%
ATCTGTCTCATAATGTTTCA+CGG + chr3_2:2684744-2684763 Msa0391170:CDS 30.0%
TCAGCAATGAAATACTTACA+TGG - chr3_2:2683695-2683714 None:intergenic 30.0%
CATTGTCTTAGAAAAAGAGA+TGG - chr3_2:2683724-2683743 None:intergenic 30.0%
!!! AAGCCAGATTGTTTTTGTTT+TGG + chr3_2:2683969-2683988 Msa0391170:intron 30.0%
CAACCAAAACAAAAACAATC+TGG - chr3_2:2683975-2683994 None:intergenic 30.0%
!! TTTACCAACAAATTTGTTGC+TGG - chr3_2:2684102-2684121 None:intergenic 30.0%
! TCAAGTTGTGAAGACTATAA+GGG - chr3_2:2684191-2684210 None:intergenic 30.0%
! ATCAAGTTGTGAAGACTATA+AGG - chr3_2:2684192-2684211 None:intergenic 30.0%
ATCTGTCTCATAATGTTTCA+CGG + chr3_2:2684744-2684763 Msa0391170:CDS 30.0%
TCTACATTCCTGTTGTACTT+AGG + chr3_2:2683662-2683681 Msa0391170:CDS 35.0%
TAGCTGCCAAAAAGATAAGA+AGG + chr3_2:2684033-2684052 Msa0391170:CDS 35.0%
! TTCGGACCTTCTTATCTTTT+TGG - chr3_2:2684042-2684061 None:intergenic 35.0%
TGCTAAGGATACGACAAATT+CGG - chr3_2:2684060-2684079 None:intergenic 35.0%
CAAGTTGTGAAGACTATAAG+GGG - chr3_2:2684190-2684209 None:intergenic 35.0%
ACTGTCGTATATAGAGAACT+TGG - chr3_2:2684249-2684268 None:intergenic 35.0%
TTAGAATTCAATATCCCGCT+TGG - chr3_2:2684311-2684330 None:intergenic 35.0%
AATATTGAGGGTAACGATGT+TGG - chr3_2:2684488-2684507 None:intergenic 35.0%
!!! GGACTTTTTTCTTTAAGTGC+TGG - chr3_2:2684533-2684552 None:intergenic 35.0%
CCATGGTATGAATCTTTCAT+TGG - chr3_2:2684554-2684573 None:intergenic 35.0%
CCAATGAAAGATTCATACCA+TGG + chr3_2:2684551-2684570 Msa0391170:CDS 35.0%
GAGATGTTTATGAGTGTCAA+GGG - chr3_2:2684620-2684639 None:intergenic 35.0%
AGAGATGTTTATGAGTGTCA+AGG - chr3_2:2684621-2684640 None:intergenic 35.0%
ATTATGAGACAGATGAACAG+AGG - chr3_2:2684738-2684757 None:intergenic 35.0%
TCTGTCTCATAATGTTTCAC+GGG + chr3_2:2684745-2684764 Msa0391170:CDS 35.0%
AGCTAAAGAGGGTAATCTTA+CGG + chr3_2:2684771-2684790 Msa0391170:CDS 35.0%
TCTACATTCCTGTTGTACTT+AGG + chr3_2:2683662-2683681 Msa0391170:CDS 35.0%
TAGCTGCCAAAAAGATAAGA+AGG + chr3_2:2684033-2684052 Msa0391170:CDS 35.0%
! TTCGGACCTTCTTATCTTTT+TGG - chr3_2:2684042-2684061 None:intergenic 35.0%
TGCTAAGGATACGACAAATT+CGG - chr3_2:2684060-2684079 None:intergenic 35.0%
CAAGTTGTGAAGACTATAAG+GGG - chr3_2:2684190-2684209 None:intergenic 35.0%
ACTGTCGTATATAGAGAACT+TGG - chr3_2:2684249-2684268 None:intergenic 35.0%
TTAGAATTCAATATCCCGCT+TGG - chr3_2:2684311-2684330 None:intergenic 35.0%
AATATTGAGGGTAACGATGT+TGG - chr3_2:2684488-2684507 None:intergenic 35.0%
!!! GGACTTTTTTCTTTAAGTGC+TGG - chr3_2:2684533-2684552 None:intergenic 35.0%
CCATGGTATGAATCTTTCAT+TGG - chr3_2:2684554-2684573 None:intergenic 35.0%
CCAATGAAAGATTCATACCA+TGG + chr3_2:2684551-2684570 Msa0391170:CDS 35.0%
GAGATGTTTATGAGTGTCAA+GGG - chr3_2:2684620-2684639 None:intergenic 35.0%
AGAGATGTTTATGAGTGTCA+AGG - chr3_2:2684621-2684640 None:intergenic 35.0%
ATTATGAGACAGATGAACAG+AGG - chr3_2:2684738-2684757 None:intergenic 35.0%
TCTGTCTCATAATGTTTCAC+GGG + chr3_2:2684745-2684764 Msa0391170:CDS 35.0%
AGCTAAAGAGGGTAATCTTA+CGG + chr3_2:2684771-2684790 Msa0391170:CDS 35.0%
ATTCCTGTTGTACTTAGGCT+TGG + chr3_2:2683667-2683686 Msa0391170:CDS 40.0%
AGAAAAATCGTCTGCTGCTA+AGG - chr3_2:2684075-2684094 None:intergenic 40.0%
CAAGCCAGCAACAAATTTGT+TGG + chr3_2:2684095-2684114 Msa0391170:CDS 40.0%
! CATATGCCAGGTCCAATTTT+TGG + chr3_2:2684219-2684238 Msa0391170:CDS 40.0%
!! GAACTTGGTGCACCAAAAAT+TGG - chr3_2:2684234-2684253 None:intergenic 40.0%
GATTGAACTGCAGCATTATC+TGG - chr3_2:2684278-2684297 None:intergenic 40.0%
CAATCAAATTGTCGACCAAG+CGG + chr3_2:2684293-2684312 Msa0391170:CDS 40.0%
AATCAAATTGTCGACCAAGC+GGG + chr3_2:2684294-2684313 Msa0391170:CDS 40.0%
AGTGATCACCTCATAGGAAA+CGG - chr3_2:2684381-2684400 None:intergenic 40.0%
AATGACAGTGATCACCTCAT+AGG - chr3_2:2684387-2684406 None:intergenic 40.0%
TTCATACCATGGAAGCCTAT+CGG + chr3_2:2684562-2684581 Msa0391170:CDS 40.0%
! ATAGAGCTTTGACTCAGTTC+TGG - chr3_2:2684593-2684612 None:intergenic 40.0%
TAGATGCCACCACACTAATA+TGG - chr3_2:2684694-2684713 None:intergenic 40.0%
ATTCCTGTTGTACTTAGGCT+TGG + chr3_2:2683667-2683686 Msa0391170:CDS 40.0%
AGAAAAATCGTCTGCTGCTA+AGG - chr3_2:2684075-2684094 None:intergenic 40.0%
CAAGCCAGCAACAAATTTGT+TGG + chr3_2:2684095-2684114 Msa0391170:CDS 40.0%
! CATATGCCAGGTCCAATTTT+TGG + chr3_2:2684219-2684238 Msa0391170:CDS 40.0%
!! GAACTTGGTGCACCAAAAAT+TGG - chr3_2:2684234-2684253 None:intergenic 40.0%
GATTGAACTGCAGCATTATC+TGG - chr3_2:2684278-2684297 None:intergenic 40.0%
CAATCAAATTGTCGACCAAG+CGG + chr3_2:2684293-2684312 Msa0391170:CDS 40.0%
AATCAAATTGTCGACCAAGC+GGG + chr3_2:2684294-2684313 Msa0391170:CDS 40.0%
AGTGATCACCTCATAGGAAA+CGG - chr3_2:2684381-2684400 None:intergenic 40.0%
AATGACAGTGATCACCTCAT+AGG - chr3_2:2684387-2684406 None:intergenic 40.0%
TTCATACCATGGAAGCCTAT+CGG + chr3_2:2684562-2684581 Msa0391170:CDS 40.0%
! ATAGAGCTTTGACTCAGTTC+TGG - chr3_2:2684593-2684612 None:intergenic 40.0%
TAGATGCCACCACACTAATA+TGG - chr3_2:2684694-2684713 None:intergenic 40.0%
TTGTGAAGACTATAAGGGGC+AGG - chr3_2:2684186-2684205 None:intergenic 45.0%
TGAACTGCAGCATTATCTGG+TGG - chr3_2:2684275-2684294 None:intergenic 45.0%
CTACATGCTGGAACTCTTCT+AGG - chr3_2:2684344-2684363 None:intergenic 45.0%
AGGATGCAGTTGATCTTTCG+TGG - chr3_2:2684416-2684435 None:intergenic 45.0%
CCTTCATAAAGAGCTGAGAC+GGG - chr3_2:2684449-2684468 None:intergenic 45.0%
CCCTTCATAAAGAGCTGAGA+CGG - chr3_2:2684450-2684469 None:intergenic 45.0%
CCGTCTCAGCTCTTTATGAA+GGG + chr3_2:2684447-2684466 Msa0391170:CDS 45.0%
CGTCTCAGCTCTTTATGAAG+GGG + chr3_2:2684448-2684467 Msa0391170:CDS 45.0%
GTCTCAGCTCTTTATGAAGG+GGG + chr3_2:2684449-2684468 Msa0391170:CDS 45.0%
!! AGTGCTGGTGAACGAGAAAA+AGG - chr3_2:2684518-2684537 None:intergenic 45.0%
TTGATCAACCGATCCAGTGT+CGG + chr3_2:2684651-2684670 Msa0391170:CDS 45.0%
GTCTCATAATGTTTCACGGG+CGG + chr3_2:2684748-2684767 Msa0391170:CDS 45.0%
TCTCATTGACGTCAAGCAGA+TGG - chr3_2:2684867-2684886 None:intergenic 45.0%
TTGTGAAGACTATAAGGGGC+AGG - chr3_2:2684186-2684205 None:intergenic 45.0%
TGAACTGCAGCATTATCTGG+TGG - chr3_2:2684275-2684294 None:intergenic 45.0%
CTACATGCTGGAACTCTTCT+AGG - chr3_2:2684344-2684363 None:intergenic 45.0%
AGGATGCAGTTGATCTTTCG+TGG - chr3_2:2684416-2684435 None:intergenic 45.0%
CCTTCATAAAGAGCTGAGAC+GGG - chr3_2:2684449-2684468 None:intergenic 45.0%
CCCTTCATAAAGAGCTGAGA+CGG - chr3_2:2684450-2684469 None:intergenic 45.0%
CCGTCTCAGCTCTTTATGAA+GGG + chr3_2:2684447-2684466 Msa0391170:CDS 45.0%
CGTCTCAGCTCTTTATGAAG+GGG + chr3_2:2684448-2684467 Msa0391170:CDS 45.0%
GTCTCAGCTCTTTATGAAGG+GGG + chr3_2:2684449-2684468 Msa0391170:CDS 45.0%
!! AGTGCTGGTGAACGAGAAAA+AGG - chr3_2:2684518-2684537 None:intergenic 45.0%
TTGATCAACCGATCCAGTGT+CGG + chr3_2:2684651-2684670 Msa0391170:CDS 45.0%
GTCTCATAATGTTTCACGGG+CGG + chr3_2:2684748-2684767 Msa0391170:CDS 45.0%
TCTCATTGACGTCAAGCAGA+TGG - chr3_2:2684867-2684886 None:intergenic 45.0%
GCTCCAAGCCTAAGTACAAC+AGG - chr3_2:2683673-2683692 None:intergenic 50.0%
GGTGCACCAAAAATTGGACC+TGG - chr3_2:2684228-2684247 None:intergenic 50.0%
CCAACAAGATGGCTACATGC+TGG - chr3_2:2684356-2684375 None:intergenic 50.0%
CCAGCATGTAGCCATCTTGT+TGG + chr3_2:2684353-2684372 Msa0391170:CDS 50.0%
! AGGAAACGGTGCCAACAAGA+TGG - chr3_2:2684367-2684386 None:intergenic 50.0%
TGTTGGCACCGTTTCCTATG+AGG + chr3_2:2684370-2684389 Msa0391170:CDS 50.0%
CCCGTCTCAGCTCTTTATGA+AGG + chr3_2:2684446-2684465 Msa0391170:CDS 50.0%
GAGGGTAACGATGTTGGAGT+CGG - chr3_2:2684482-2684501 None:intergenic 50.0%
! TCAGTTCTGGTAGCTCCGAT+AGG - chr3_2:2684580-2684599 None:intergenic 50.0%
TGATCAACCGATCCAGTGTC+GGG + chr3_2:2684652-2684671 Msa0391170:CDS 50.0%
TCAAGCAGATGGCAAAGGCT+TGG - chr3_2:2684856-2684875 None:intergenic 50.0%
TGACGTCAAGCAGATGGCAA+AGG - chr3_2:2684861-2684880 None:intergenic 50.0%
GCTCCAAGCCTAAGTACAAC+AGG - chr3_2:2683673-2683692 None:intergenic 50.0%
GGTGCACCAAAAATTGGACC+TGG - chr3_2:2684228-2684247 None:intergenic 50.0%
CCAACAAGATGGCTACATGC+TGG - chr3_2:2684356-2684375 None:intergenic 50.0%
CCAGCATGTAGCCATCTTGT+TGG + chr3_2:2684353-2684372 Msa0391170:CDS 50.0%
! AGGAAACGGTGCCAACAAGA+TGG - chr3_2:2684367-2684386 None:intergenic 50.0%
TGTTGGCACCGTTTCCTATG+AGG + chr3_2:2684370-2684389 Msa0391170:CDS 50.0%
CCCGTCTCAGCTCTTTATGA+AGG + chr3_2:2684446-2684465 Msa0391170:CDS 50.0%
GAGGGTAACGATGTTGGAGT+CGG - chr3_2:2684482-2684501 None:intergenic 50.0%
! TCAGTTCTGGTAGCTCCGAT+AGG - chr3_2:2684580-2684599 None:intergenic 50.0%
TGATCAACCGATCCAGTGTC+GGG + chr3_2:2684652-2684671 Msa0391170:CDS 50.0%
TCAAGCAGATGGCAAAGGCT+TGG - chr3_2:2684856-2684875 None:intergenic 50.0%
TGACGTCAAGCAGATGGCAA+AGG - chr3_2:2684861-2684880 None:intergenic 50.0%
!! CTGAGACGGGTATCGAGTTC+AGG - chr3_2:2684436-2684455 None:intergenic 55.0%
GTAGCTCCGATAGGCTTCCA+TGG - chr3_2:2684571-2684590 None:intergenic 55.0%
GATCAACCGATCCAGTGTCG+GGG + chr3_2:2684653-2684672 Msa0391170:CDS 55.0%
CACCACACTAATATGGCCCG+CGG - chr3_2:2684687-2684706 None:intergenic 55.0%
TTTCACGGGCGGAGCTAAAG+AGG + chr3_2:2684759-2684778 Msa0391170:CDS 55.0%
TTCACGGGCGGAGCTAAAGA+GGG + chr3_2:2684760-2684779 Msa0391170:CDS 55.0%
AAGGCTTGGAAGGCAGAGAG+TGG - chr3_2:2684842-2684861 None:intergenic 55.0%
GCAGATGGCAAAGGCTTGGA+AGG - chr3_2:2684852-2684871 None:intergenic 55.0%
!! CTGAGACGGGTATCGAGTTC+AGG - chr3_2:2684436-2684455 None:intergenic 55.0%
GTAGCTCCGATAGGCTTCCA+TGG - chr3_2:2684571-2684590 None:intergenic 55.0%
GATCAACCGATCCAGTGTCG+GGG + chr3_2:2684653-2684672 Msa0391170:CDS 55.0%
CACCACACTAATATGGCCCG+CGG - chr3_2:2684687-2684706 None:intergenic 55.0%
TTTCACGGGCGGAGCTAAAG+AGG + chr3_2:2684759-2684778 Msa0391170:CDS 55.0%
TTCACGGGCGGAGCTAAAGA+GGG + chr3_2:2684760-2684779 Msa0391170:CDS 55.0%
AAGGCTTGGAAGGCAGAGAG+TGG - chr3_2:2684842-2684861 None:intergenic 55.0%
GCAGATGGCAAAGGCTTGGA+AGG - chr3_2:2684852-2684871 None:intergenic 55.0%
CATCGTCCCCGACACTGGAT+CGG - chr3_2:2684662-2684681 None:intergenic 60.0%
CTCCGCGGGCCATATTAGTG+TGG + chr3_2:2684682-2684701 Msa0391170:CDS 60.0%
CGCGGGCCATATTAGTGTGG+TGG + chr3_2:2684685-2684704 Msa0391170:CDS 60.0%
CATCGTCCCCGACACTGGAT+CGG - chr3_2:2684662-2684681 None:intergenic 60.0%
CTCCGCGGGCCATATTAGTG+TGG + chr3_2:2684682-2684701 Msa0391170:CDS 60.0%
CGCGGGCCATATTAGTGTGG+TGG + chr3_2:2684685-2684704 Msa0391170:CDS 60.0%
!! CGGAGCATCGTCCCCGACAC+TGG - chr3_2:2684667-2684686 None:intergenic 70.0%
GTGTCGGGGACGATGCTCCG+CGG + chr3_2:2684667-2684686 Msa0391170:CDS 70.0%
TGTCGGGGACGATGCTCCGC+GGG + chr3_2:2684668-2684687 Msa0391170:CDS 70.0%
!! CGGAGCATCGTCCCCGACAC+TGG - chr3_2:2684667-2684686 None:intergenic 70.0%
GTGTCGGGGACGATGCTCCG+CGG + chr3_2:2684667-2684686 Msa0391170:CDS 70.0%
TGTCGGGGACGATGCTCCGC+GGG + chr3_2:2684668-2684687 Msa0391170:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 2683647 2684901 2683647 ID=Msa0391170;Name=Msa0391170
chr3_2 mRNA 2683647 2684901 2683647 ID=Msa0391170-mRNA-1;Parent=Msa0391170;Name=Msa0391170-mRNA-1;_AED=0.05;_eAED=0.08;_QI=0|0|0|1|1|1|3|0|289
chr3_2 exon 2683647 2683695 2683647 ID=Msa0391170-mRNA-1:exon:779;Parent=Msa0391170-mRNA-1
chr3_2 exon 2684006 2684121 2684006 ID=Msa0391170-mRNA-1:exon:780;Parent=Msa0391170-mRNA-1
chr3_2 exon 2684197 2684901 2684197 ID=Msa0391170-mRNA-1:exon:781;Parent=Msa0391170-mRNA-1
chr3_2 CDS 2683647 2683695 2683647 ID=Msa0391170-mRNA-1:cds;Parent=Msa0391170-mRNA-1
chr3_2 CDS 2684006 2684121 2684006 ID=Msa0391170-mRNA-1:cds;Parent=Msa0391170-mRNA-1
chr3_2 CDS 2684197 2684901 2684197 ID=Msa0391170-mRNA-1:cds;Parent=Msa0391170-mRNA-1
Gene Sequence

>Msa0391170

ATGAACAAAAGAACGTCTACATTCCTGTTGTACTTAGGCTTGGAGCCATCGAATAATGAGAGTAATAAGTTGTTATTAGCTGCCAAAAAGATAAGAAGGTCCGAATTTGTCGTATCCTTAGCAGCAGACGATTTTTCTCAAGCCAGCAACAAATTTGTTGGTAAATCTTCACAACTTGATTTTTATACATATGCCAGGTCCAATTTTTGGTGCACCAAGTTCTCTATATACGACAGTCAACCACCAGATAATGCTGCAGTTCAATCAAATTGTCGACCAAGCGGGATATTGAATTCTAAGCAAGTGTCACCTAGAAGAGTTCCAGCATGTAGCCATCTTGTTGGCACCGTTTCCTATGAGGTGATCACTGTCATTGATACACCACGAAAGATCAACTGCATCCTGAACTCGATACCCGTCTCAGCTCTTTATGAAGGGGGAAACACTCCGACTCCAACATCGTTACCCTCAATATTTGATATACCTTTTTCTCGTTCACCAGCACTTAAAGAAAAAAGTCCAATGAAAGATTCATACCATGGAAGCCTATCGGAGCTACCAGAACTGAGTCAAAGCTCTATCGAGCCCTTGACACTCATAAACATCTCTCGCTCATATTTTGATCAACCGATCCAGTGTCGGGGACGATGCTCCGCGGGCCATATTAGTGTGGTGGCATCTATAAAGAACTTTCAACTTGGAGCCTCTGTTCATCTGTCTCATAATGTTTCACGGGCGGAGCTAAAGAGGGTAATCTTACGGTTTGCAAAGATTGAAAAAGATCTATTCATCATGGACTACTCTTATCCACTCTCTGCCTTCCAAGCCTTTGCCATCTGCTTGACGTCAATGAGAGCAATACATTTATGA
Protein sequence

>Msa0391170

MNKRTSTFLLYLGLEPSNNESNKLLLAAKKIRRSEFVVSLAADDFSQASNKFVGKSSQLDFYTYARSNFWCTKFSIYDSQPPDNAAVQSNCRPSGILNSKQVSPRRVPACSHLVGTVSYEVITVIDTPRKINCILNSIPVSALYEGGNTPTPTSLPSIFDIPFSRSPALKEKSPMKDSYHGSLSELPELSQSSIEPLTLINISRSYFDQPIQCRGRCSAGHISVVASIKNFQLGASVHLSHNVSRAELKRVILRFAKIEKDLFIMDYSYPLSAFQAFAICLTSMRAIHL*