AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0391920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0391920 MtrunA17_Chr3g0090311 56.284 183 51 5 4 158 2 183 1.34e-57 177
Msa0391920 MtrunA17_Chr3g0098461 36.646 161 95 4 4 159 6 164 1.71e-18 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0391920 AT5G13780.1 53.548 155 59 4 4 146 2 155 2.93e-47 152

Find 39 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCTGTTCCATGTCGTTT+TGG 0.275526 3_2:+4836915 None:intergenic
AAGGGGAAGCAGATGCATTC+TGG 0.323040 3_2:-4836027 Msa0391920:CDS
TCTAAGATGTACAAACTCTT+TGG 0.380946 3_2:-4837124 Msa0391920:CDS
ACATTGGGATACAAGATTCA+TGG 0.416619 3_2:-4836111 Msa0391920:CDS
AGGGGAAGCAGATGCATTCT+GGG 0.418741 3_2:-4836026 Msa0391920:CDS
GGCCGTGAGAAGAGGGCAAC+AGG 0.424430 3_2:+4837094 None:intergenic
CTCCCAAAACGACATGGAAC+AGG 0.433249 3_2:-4836918 Msa0391920:intron
TGCATTCTGGGGAGACTAAA+GGG 0.439115 3_2:-4836014 Msa0391920:CDS
TTGTATTTAACACAGATGTT+TGG 0.469950 3_2:-4836198 Msa0391920:intron
GGGATACAAGATTCATGGAT+TGG 0.477746 3_2:-4836106 Msa0391920:CDS
CACGTCGTAGAAGATATTAA+TGG 0.486544 3_2:-4837061 Msa0391920:CDS
TAATTTGTTCAAAGAGACAT+TGG 0.499278 3_2:-4836127 Msa0391920:CDS
TCTCTGATGGGTTGGGAGAA+CGG 0.508987 3_2:+4836970 None:intergenic
ATGCATTCTGGGGAGACTAA+AGG 0.509032 3_2:-4836015 Msa0391920:CDS
TACCACGTCTCTGATGGGTT+GGG 0.526888 3_2:+4836963 None:intergenic
AATTTGTTCAAAGAGACATT+GGG 0.527731 3_2:-4836126 Msa0391920:CDS
TAGCGATACCACGTCTCTGA+TGG 0.539259 3_2:+4836957 None:intergenic
ATACCACGTCTCTGATGGGT+TGG 0.540598 3_2:+4836962 None:intergenic
GAGTTGAGGCCGTGAGAAGA+GGG 0.546949 3_2:+4837087 None:intergenic
TGAACAAATTAAAAGCTGCA+TGG 0.554368 3_2:+4836139 None:intergenic
ATGGGTTGGGAGAACGGCGA+GGG 0.570608 3_2:+4836976 None:intergenic
GAAATGAGAAAGCAACTCAA+GGG 0.572712 3_2:-4836045 Msa0391920:CDS
TGAGTTGAGGCCGTGAGAAG+AGG 0.574852 3_2:+4837086 None:intergenic
TGAAATGAGAAAGCAACTCA+AGG 0.580702 3_2:-4836046 Msa0391920:CDS
AGCGATACCACGTCTCTGAT+GGG 0.588160 3_2:+4836958 None:intergenic
GATTGCCTCCCAAAACGACA+TGG 0.596635 3_2:-4836924 Msa0391920:CDS
ATTCAAGAGGAAATTAGTGA+AGG 0.600654 3_2:-4837010 Msa0391920:CDS
AGCCTGTTGCCCTCTTCTCA+CGG 0.606181 3_2:-4837096 Msa0391920:CDS
GATGGGTTGGGAGAACGGCG+AGG 0.612867 3_2:+4836975 None:intergenic
CGTCCACTGTGAAATTCAAG+AGG 0.617845 3_2:-4837023 Msa0391920:CDS
ATACAAGATTCATGGATTGG+AGG 0.623983 3_2:-4836103 Msa0391920:CDS
TTGAATTTCACAGTGGACGT+AGG 0.625067 3_2:+4837027 None:intergenic
AGAGTTTGTACATCTTAGAG+AGG 0.637261 3_2:+4837128 None:intergenic
CTCCCAACCCATCAGAGACG+TGG 0.645269 3_2:-4836965 Msa0391920:CDS
AAATGAGAAAGCAACTCAAG+GGG 0.646235 3_2:-4836044 Msa0391920:CDS
GGGGAAGCAGATGCATTCTG+GGG 0.660086 3_2:-4836025 Msa0391920:CDS
ACATCTTAGAGAGGTCGCAA+AGG 0.660670 3_2:+4837137 None:intergenic
TCTACGACGTGAATGAGTTG+AGG 0.674386 3_2:+4837073 None:intergenic
TTTCCTCTTGAATTTCACAG+TGG 0.694893 3_2:+4837020 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATGTATCTTTTATATT+TGG - chr3_2:4836670-4836689 Msa0391920:intron 10.0%
!!! TAATATGTATCTTTTATATT+TGG - chr3_2:4836670-4836689 Msa0391920:intron 10.0%
!! AAAATGATCCTTAATTATTT+CGG + chr3_2:4836621-4836640 None:intergenic 15.0%
!! TTCAAGAAAATTATTTGAAT+AGG + chr3_2:4836819-4836838 None:intergenic 15.0%
!! AAAATGATCCTTAATTATTT+CGG + chr3_2:4836621-4836640 None:intergenic 15.0%
!! TTCAAGAAAATTATTTGAAT+AGG + chr3_2:4836819-4836838 None:intergenic 15.0%
!!! ATTTTTAGCCGAAATAATTA+AGG - chr3_2:4836610-4836629 Msa0391920:intron 20.0%
!!! ATTTTTAGCCGAAATAATTA+AGG - chr3_2:4836610-4836629 Msa0391920:intron 20.0%
! ATCAAATTAATCAATGCATC+TGG - chr3_2:4836535-4836554 Msa0391920:intron 25.0%
!!! ACTTGTTACAGTTTTGTTAT+TGG - chr3_2:4836562-4836581 Msa0391920:intron 25.0%
! TTGTATTTAACACAGATGTT+TGG - chr3_2:4836964-4836983 Msa0391920:CDS 25.0%
!!! TAATTTGTTCAAAGAGACAT+TGG - chr3_2:4837035-4837054 Msa0391920:CDS 25.0%
!! AATTTGTTCAAAGAGACATT+GGG - chr3_2:4837036-4837055 Msa0391920:CDS 25.0%
! ATCAAATTAATCAATGCATC+TGG - chr3_2:4836535-4836554 Msa0391920:intron 25.0%
!!! ACTTGTTACAGTTTTGTTAT+TGG - chr3_2:4836562-4836581 Msa0391920:intron 25.0%
! TTGTATTTAACACAGATGTT+TGG - chr3_2:4836964-4836983 Msa0391920:CDS 25.0%
!!! TAATTTGTTCAAAGAGACAT+TGG - chr3_2:4837035-4837054 Msa0391920:CDS 25.0%
!! AATTTGTTCAAAGAGACATT+GGG - chr3_2:4837036-4837055 Msa0391920:CDS 25.0%
! TCTAAGATGTACAAACTCTT+TGG - chr3_2:4836038-4836057 Msa0391920:CDS 30.0%
ATTCAAGAGGAAATTAGTGA+AGG - chr3_2:4836152-4836171 Msa0391920:CDS 30.0%
TATTAATTCAAATTGCACCG+AGG - chr3_2:4836319-4836338 Msa0391920:intron 30.0%
ATTAATTCAAATTGCACCGA+GGG - chr3_2:4836320-4836339 Msa0391920:intron 30.0%
GAGTCAAGTTATTAACTACT+TGG - chr3_2:4836704-4836723 Msa0391920:intron 30.0%
ATATGTAGAATTGCAGAACT+AGG + chr3_2:4836857-4836876 None:intergenic 30.0%
TGTTATGAGTTAGATTCATC+AGG - chr3_2:4836876-4836895 Msa0391920:intron 30.0%
AAGTGTGTCTTCTTGTAATA+GGG - chr3_2:4836910-4836929 Msa0391920:intron 30.0%
TGAACAAATTAAAAGCTGCA+TGG + chr3_2:4837026-4837045 None:intergenic 30.0%
!! CAAAGTTTTATGCTGATGAT+GGG - chr3_2:4837082-4837101 Msa0391920:CDS 30.0%
!! AAAGTTTTATGCTGATGATG+GGG - chr3_2:4837083-4837102 Msa0391920:CDS 30.0%
! TCTAAGATGTACAAACTCTT+TGG - chr3_2:4836038-4836057 Msa0391920:CDS 30.0%
ATTCAAGAGGAAATTAGTGA+AGG - chr3_2:4836152-4836171 Msa0391920:CDS 30.0%
TATTAATTCAAATTGCACCG+AGG - chr3_2:4836319-4836338 Msa0391920:intron 30.0%
ATTAATTCAAATTGCACCGA+GGG - chr3_2:4836320-4836339 Msa0391920:intron 30.0%
GAGTCAAGTTATTAACTACT+TGG - chr3_2:4836704-4836723 Msa0391920:intron 30.0%
ATATGTAGAATTGCAGAACT+AGG + chr3_2:4836857-4836876 None:intergenic 30.0%
TGTTATGAGTTAGATTCATC+AGG - chr3_2:4836876-4836895 Msa0391920:intron 30.0%
AAGTGTGTCTTCTTGTAATA+GGG - chr3_2:4836910-4836929 Msa0391920:intron 30.0%
TGAACAAATTAAAAGCTGCA+TGG + chr3_2:4837026-4837045 None:intergenic 30.0%
!! CAAAGTTTTATGCTGATGAT+GGG - chr3_2:4837082-4837101 Msa0391920:CDS 30.0%
!! AAAGTTTTATGCTGATGATG+GGG - chr3_2:4837083-4837102 Msa0391920:CDS 30.0%
AGAGTTTGTACATCTTAGAG+AGG + chr3_2:4836037-4836056 None:intergenic 35.0%
CACGTCGTAGAAGATATTAA+TGG - chr3_2:4836101-4836120 Msa0391920:CDS 35.0%
TTTCCTCTTGAATTTCACAG+TGG + chr3_2:4836145-4836164 None:intergenic 35.0%
GAAGTGTGTCTTCTTGTAAT+AGG - chr3_2:4836909-4836928 Msa0391920:intron 35.0%
ACATTGGGATACAAGATTCA+TGG - chr3_2:4837051-4837070 Msa0391920:CDS 35.0%
!! ATACAAGATTCATGGATTGG+AGG - chr3_2:4837059-4837078 Msa0391920:CDS 35.0%
!! GCAAAGTTTTATGCTGATGA+TGG - chr3_2:4837081-4837100 Msa0391920:CDS 35.0%
TGAAATGAGAAAGCAACTCA+AGG - chr3_2:4837116-4837135 Msa0391920:CDS 35.0%
GAAATGAGAAAGCAACTCAA+GGG - chr3_2:4837117-4837136 Msa0391920:CDS 35.0%
AAATGAGAAAGCAACTCAAG+GGG - chr3_2:4837118-4837137 Msa0391920:CDS 35.0%
AGAGTTTGTACATCTTAGAG+AGG + chr3_2:4836037-4836056 None:intergenic 35.0%
CACGTCGTAGAAGATATTAA+TGG - chr3_2:4836101-4836120 Msa0391920:CDS 35.0%
TTTCCTCTTGAATTTCACAG+TGG + chr3_2:4836145-4836164 None:intergenic 35.0%
GAAGTGTGTCTTCTTGTAAT+AGG - chr3_2:4836909-4836928 Msa0391920:intron 35.0%
ACATTGGGATACAAGATTCA+TGG - chr3_2:4837051-4837070 Msa0391920:CDS 35.0%
!! ATACAAGATTCATGGATTGG+AGG - chr3_2:4837059-4837078 Msa0391920:CDS 35.0%
!! GCAAAGTTTTATGCTGATGA+TGG - chr3_2:4837081-4837100 Msa0391920:CDS 35.0%
TGAAATGAGAAAGCAACTCA+AGG - chr3_2:4837116-4837135 Msa0391920:CDS 35.0%
GAAATGAGAAAGCAACTCAA+GGG - chr3_2:4837117-4837136 Msa0391920:CDS 35.0%
AAATGAGAAAGCAACTCAAG+GGG - chr3_2:4837118-4837137 Msa0391920:CDS 35.0%
TTGAATTTCACAGTGGACGT+AGG + chr3_2:4836138-4836157 None:intergenic 40.0%
!!! AACCTGTTCCATGTCGTTTT+GGG + chr3_2:4836249-4836268 None:intergenic 40.0%
!! AAACCTGTTCCATGTCGTTT+TGG + chr3_2:4836250-4836269 None:intergenic 40.0%
GAAACTGAGTTTGAATCCCT+CGG + chr3_2:4836339-4836358 None:intergenic 40.0%
! CTCAGTTTCTGAGAGTTACA+AGG - chr3_2:4836350-4836369 Msa0391920:intron 40.0%
GGGATACAAGATTCATGGAT+TGG - chr3_2:4837056-4837075 Msa0391920:CDS 40.0%
TTGAATTTCACAGTGGACGT+AGG + chr3_2:4836138-4836157 None:intergenic 40.0%
!!! AACCTGTTCCATGTCGTTTT+GGG + chr3_2:4836249-4836268 None:intergenic 40.0%
!! AAACCTGTTCCATGTCGTTT+TGG + chr3_2:4836250-4836269 None:intergenic 40.0%
GAAACTGAGTTTGAATCCCT+CGG + chr3_2:4836339-4836358 None:intergenic 40.0%
! CTCAGTTTCTGAGAGTTACA+AGG - chr3_2:4836350-4836369 Msa0391920:intron 40.0%
GGGATACAAGATTCATGGAT+TGG - chr3_2:4837056-4837075 Msa0391920:CDS 40.0%
ACATCTTAGAGAGGTCGCAA+AGG + chr3_2:4836028-4836047 None:intergenic 45.0%
TCTACGACGTGAATGAGTTG+AGG + chr3_2:4836092-4836111 None:intergenic 45.0%
CGTCCACTGTGAAATTCAAG+AGG - chr3_2:4836139-4836158 Msa0391920:CDS 45.0%
!!! GTTTTATGCTGATGATGGGG+AGG - chr3_2:4837086-4837105 Msa0391920:CDS 45.0%
! ATGCATTCTGGGGAGACTAA+AGG - chr3_2:4837147-4837166 Msa0391920:CDS 45.0%
! TGCATTCTGGGGAGACTAAA+GGG - chr3_2:4837148-4837167 Msa0391920:CDS 45.0%
ACATCTTAGAGAGGTCGCAA+AGG + chr3_2:4836028-4836047 None:intergenic 45.0%
TCTACGACGTGAATGAGTTG+AGG + chr3_2:4836092-4836111 None:intergenic 45.0%
CGTCCACTGTGAAATTCAAG+AGG - chr3_2:4836139-4836158 Msa0391920:CDS 45.0%
!!! GTTTTATGCTGATGATGGGG+AGG - chr3_2:4837086-4837105 Msa0391920:CDS 45.0%
! ATGCATTCTGGGGAGACTAA+AGG - chr3_2:4837147-4837166 Msa0391920:CDS 45.0%
! TGCATTCTGGGGAGACTAAA+GGG - chr3_2:4837148-4837167 Msa0391920:CDS 45.0%
!! TCTCTGATGGGTTGGGAGAA+CGG + chr3_2:4836195-4836214 None:intergenic 50.0%
!! TACCACGTCTCTGATGGGTT+GGG + chr3_2:4836202-4836221 None:intergenic 50.0%
! ATACCACGTCTCTGATGGGT+TGG + chr3_2:4836203-4836222 None:intergenic 50.0%
AGCGATACCACGTCTCTGAT+GGG + chr3_2:4836207-4836226 None:intergenic 50.0%
TAGCGATACCACGTCTCTGA+TGG + chr3_2:4836208-4836227 None:intergenic 50.0%
GATTGCCTCCCAAAACGACA+TGG - chr3_2:4836238-4836257 Msa0391920:intron 50.0%
!!! CTGTTCCATGTCGTTTTGGG+AGG + chr3_2:4836246-4836265 None:intergenic 50.0%
CTCCCAAAACGACATGGAAC+AGG - chr3_2:4836244-4836263 Msa0391920:intron 50.0%
AAGGGGAAGCAGATGCATTC+TGG - chr3_2:4837135-4837154 Msa0391920:CDS 50.0%
AGGGGAAGCAGATGCATTCT+GGG - chr3_2:4837136-4837155 Msa0391920:CDS 50.0%
!! TCTCTGATGGGTTGGGAGAA+CGG + chr3_2:4836195-4836214 None:intergenic 50.0%
!! TACCACGTCTCTGATGGGTT+GGG + chr3_2:4836202-4836221 None:intergenic 50.0%
! ATACCACGTCTCTGATGGGT+TGG + chr3_2:4836203-4836222 None:intergenic 50.0%
AGCGATACCACGTCTCTGAT+GGG + chr3_2:4836207-4836226 None:intergenic 50.0%
TAGCGATACCACGTCTCTGA+TGG + chr3_2:4836208-4836227 None:intergenic 50.0%
GATTGCCTCCCAAAACGACA+TGG - chr3_2:4836238-4836257 Msa0391920:intron 50.0%
!!! CTGTTCCATGTCGTTTTGGG+AGG + chr3_2:4836246-4836265 None:intergenic 50.0%
CTCCCAAAACGACATGGAAC+AGG - chr3_2:4836244-4836263 Msa0391920:intron 50.0%
AAGGGGAAGCAGATGCATTC+TGG - chr3_2:4837135-4837154 Msa0391920:CDS 50.0%
AGGGGAAGCAGATGCATTCT+GGG - chr3_2:4837136-4837155 Msa0391920:CDS 50.0%
AGCCTGTTGCCCTCTTCTCA+CGG - chr3_2:4836066-4836085 Msa0391920:CDS 55.0%
GAGTTGAGGCCGTGAGAAGA+GGG + chr3_2:4836078-4836097 None:intergenic 55.0%
! TGAGTTGAGGCCGTGAGAAG+AGG + chr3_2:4836079-4836098 None:intergenic 55.0%
GGGGAAGCAGATGCATTCTG+GGG - chr3_2:4837137-4837156 Msa0391920:CDS 55.0%
AGCCTGTTGCCCTCTTCTCA+CGG - chr3_2:4836066-4836085 Msa0391920:CDS 55.0%
GAGTTGAGGCCGTGAGAAGA+GGG + chr3_2:4836078-4836097 None:intergenic 55.0%
! TGAGTTGAGGCCGTGAGAAG+AGG + chr3_2:4836079-4836098 None:intergenic 55.0%
GGGGAAGCAGATGCATTCTG+GGG - chr3_2:4837137-4837156 Msa0391920:CDS 55.0%
!! ATGGGTTGGGAGAACGGCGA+GGG + chr3_2:4836189-4836208 None:intergenic 60.0%
CTCCCAACCCATCAGAGACG+TGG - chr3_2:4836197-4836216 Msa0391920:intron 60.0%
!! ATGGGTTGGGAGAACGGCGA+GGG + chr3_2:4836189-4836208 None:intergenic 60.0%
CTCCCAACCCATCAGAGACG+TGG - chr3_2:4836197-4836216 Msa0391920:intron 60.0%
GGCCGTGAGAAGAGGGCAAC+AGG + chr3_2:4836071-4836090 None:intergenic 65.0%
!! GATGGGTTGGGAGAACGGCG+AGG + chr3_2:4836190-4836209 None:intergenic 65.0%
GGCCGTGAGAAGAGGGCAAC+AGG + chr3_2:4836071-4836090 None:intergenic 65.0%
!! GATGGGTTGGGAGAACGGCG+AGG + chr3_2:4836190-4836209 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 4835996 4837188 4835996 ID=Msa0391920;Name=Msa0391920
chr3_2 mRNA 4835996 4837188 4835996 ID=Msa0391920-mRNA-1;Parent=Msa0391920;Name=Msa0391920-mRNA-1;_AED=0.07;_eAED=0.08;_QI=0|0|0|1|1|1|2|0|159
chr3_2 exon 4836919 4837188 4836919 ID=Msa0391920-mRNA-1:exon:1155;Parent=Msa0391920-mRNA-1
chr3_2 exon 4835996 4836205 4835996 ID=Msa0391920-mRNA-1:exon:1154;Parent=Msa0391920-mRNA-1
chr3_2 CDS 4836919 4837188 4836919 ID=Msa0391920-mRNA-1:cds;Parent=Msa0391920-mRNA-1
chr3_2 CDS 4835996 4836205 4835996 ID=Msa0391920-mRNA-1:cds;Parent=Msa0391920-mRNA-1
Gene Sequence

>Msa0391920

ATGTCTCAAGTGTGCATAAGAGAAGCAAACCTTTGCGACCTCTCTAAGATGTACAAACTCTTTGGTCTAAAGCCTGTTGCCCTCTTCTCACGGCCTCAACTCATTCACGTCGTAGAAGATATTAATGGTCACATCGTCGCCTACGTCCACTGTGAAATTCAAGAGGAAATTAGTGAAGGAGCTATTCATTCCCTCGCCGTTCTCCCAACCCATCAGAGACGTGGTATCGCTACTAAGCTTGTGATTGCCTCCCAAAACGACATGGAACAGATGTTTGGTTCTGAGTACGTCTCAGTGCATGTCAGAAAGAGCAACCATGCAGCTTTTAATTTGTTCAAAGAGACATTGGGATACAAGATTCATGGATTGGAGGCAAAGTTTTATGCTGATGATGGGGAGGATGCTTATGAAATGAGAAAGCAACTCAAGGGGAAGCAGATGCATTCTGGGGAGACTAAAGGGAATGCAAAAGCCACATGA

Protein sequence

>Msa0391920

MSQVCIREANLCDLSKMYKLFGLKPVALFSRPQLIHVVEDINGHIVAYVHCEIQEEISEGAIHSLAVLPTHQRRGIATKLVIASQNDMEQMFGSEYVSVHVRKSNHAAFNLFKETLGYKIHGLEAKFYADDGEDAYEMRKQLKGKQMHSGETKGNAKAT*