AlfalfaGEDB | Msa0392690

M. sativa cultivar zhongmu-4 / Msa0392690

KEGG: -
GO: -
PFAM: PF02671.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0392690	XP_039688272.1	93.177	469	32	0	1	469	1	469	0.0	881

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0392690	sp\|O04539\|SNL4_ARATH	51.948	77	36	1	2	78	8	83	2.87e-14	79.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0392690	G7J085	93.177	469	32	0	1	469	1	469	0.0	881

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TFs/TRs:

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Gene ID	Type	Classification
Msa0392690	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0392690	MtrunA17_Chr3g0077801	94.034	352	21	0	118	469	1	352	0.0	692
Msa0392690	MtrunA17_Chr3g0077901	68.750	352	110	0	114	465	17	368	0.0	514
Msa0392690	MtrunA17_Chr3g0077851	72.590	332	91	0	134	465	1	332	2.47e-180	507
Msa0392690	MtrunA17_Chr4g0075971	58.553	456	158	5	11	466	175	599	3.88e-173	499
Msa0392690	MtrunA17_Chr4g0075971	33.824	136	80	3	6	141	10	135	2.38e-12	69.3
Msa0392690	MtrunA17_Chr6g0488101	47.095	482	209	10	1	466	1	452	6.57e-141	412
Msa0392690	MtrunA17_Chr6g0488271	48.526	441	189	8	35	466	1	412	1.88e-135	396
Msa0392690	MtrunA17_Chr3g0112311	56.111	360	145	4	118	468	1	356	1.19e-130	382
Msa0392690	MtrunA17_Chr7g0252101	48.268	462	185	10	11	466	16	429	1.46e-126	374
Msa0392690	MtrunA17_Chr3g0093911	55.807	353	146	5	118	466	1	347	1.49e-123	363

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0392690	AT5G15025.1	47.059	85	40	2	4	88	43	122	1.77e-15	73.2
Msa0392690	AT1G70060.2	51.948	77	36	1	2	78	8	83	2.91e-15	79.3
Msa0392690	AT1G70060.1	51.948	77	36	1	2	78	8	83	2.91e-15	79.3
Msa0392690	AT1G24190.3	48.052	77	39	1	2	78	8	83	4.88e-14	75.5
Msa0392690	AT1G24190.1	48.052	77	39	1	2	78	8	83	5.05e-14	75.5
Msa0392690	AT1G24190.2	48.052	77	39	1	2	78	8	83	5.09e-14	75.5
Msa0392690	AT3G01320.1	48.193	83	41	2	2	84	51	131	2.80e-13	73.2
Msa0392690	AT3G01320.2	48.193	83	41	2	2	84	51	131	2.90e-13	72.8
Msa0392690	AT1G24200.1	50.000	72	35	1	5	76	11	81	1.43e-12	67.0
Msa0392690	AT1G70030.3	44.444	81	44	1	2	82	16	95	1.49e-12	65.1

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Find 87 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCCTACTTGCTGGAT+TGG	0.490737	3_2:-5922224	Msa0392690:CDS
AAAGCACCAATCCAGCAAGT+AGG	0.620337	3_2:+5922218	None:intergenic
AAAGGAAAATGCCTACTTGC+TGG	0.383831	3_2:-5922229	Msa0392690:CDS
AACAATGGGAAATCCATGGA+AGG	0.592110	3_2:-5922691	Msa0392690:CDS
AACATGTAAAGTGTGTTTAG+GGG	0.701783	3_2:-5922304	Msa0392690:CDS
AACCATGAATTATGTCAAGC+TGG	0.505920	3_2:-5922445	Msa0392690:CDS
AATCACACCTTCAGTTCAGC+TGG	0.522086	3_2:-5924727	Msa0392690:CDS
AATGACATCAAGCATATCCC+AGG	0.580022	3_2:+5923181	None:intergenic
ACAAAGTCAACAACCTTCCA+TGG	0.493618	3_2:+5922678	None:intergenic
ACAAATTATGAAGGATTACA+AGG	0.516015	3_2:-5924946	Msa0392690:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTAAGTTTGGATATGAGAAA+AGG	-	chr3_2:5922228-5922247	Msa0392690:CDS	30.0%
TTCATATTTAGTTGGCAAGA+AGG	+	chr3_2:5922381-5922400	None:intergenic	30.0%
ATGTCAAGTAGAATAATGGT+AGG	+	chr3_2:5922530-5922549	None:intergenic	30.0%
ACACATGTCAAGTAGAATAA+TGG	+	chr3_2:5922534-5922553	None:intergenic	30.0%
TGACAGATTATAGCAAAAAG+CGG	+	chr3_2:5922658-5922677	None:intergenic	30.0%
AGAAGTGAGTGTAAGATTAA+GGG	-	chr3_2:5922731-5922750	Msa0392690:CDS	30.0%
CTCATAGTTTAACGATAAGT+TGG	-	chr3_2:5922790-5922809	Msa0392690:CDS	30.0%
TTAGGGGAAATCTTGTAAAT+TGG	-	chr3_2:5922829-5922848	Msa0392690:CDS	30.0%
ATATGACATTGATCAACCAT+AGG	-	chr3_2:5923185-5923204	Msa0392690:CDS	30.0%
TGATGTAACAAAGATATGGA+AGG	-	chr3_2:5923337-5923356	Msa0392690:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	5922176	5925063	5922176	ID=Msa0392690;Name=Msa0392690
chr3_2	mRNA	5922176	5925063	5922176	ID=Msa0392690-mRNA-1;Parent=Msa0392690;Name=Msa0392690-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0\|0\|0\|1\|1\|1\|2\|0\|469
chr3_2	exon	5924721	5925063	5924721	ID=Msa0392690-mRNA-1:exon:1535;Parent=Msa0392690-mRNA-1
chr3_2	exon	5922176	5923242	5922176	ID=Msa0392690-mRNA-1:exon:1534;Parent=Msa0392690-mRNA-1
chr3_2	CDS	5924721	5925063	5924721	ID=Msa0392690-mRNA-1:cds;Parent=Msa0392690-mRNA-1
chr3_2	CDS	5922176	5923242	5922176	ID=Msa0392690-mRNA-1:cds;Parent=Msa0392690-mRNA-1

Gene Sequence

>Msa0392690

ATGAAGAATCTCTCCCTTGATGATGCATTAGGGTTAATCTCAGCATTAAAATGTAAGTTTGGATATGAGAAAAGGGAAAAATATGAATCTTTTCTACAAATTATGAAGGATTACAAGGCTGAAAGAATTGATGCTAGGGTTGTCAAATTAAGAGTGTACGAGTTGTTGGACGGCCATGAAGATTTAATTTTGAGATTCAACACCTTCTTGCCAACTAAATATGAAATCAAACTTCCATTGGATCGTGATGACGATAATGAAGAACAAGATGGTCGAATGACAAAGACAAATATCAAAGTGCTAAAGAAATATCGAATCACACCTTCAGTTCAGCTGGACACTGGGGAAGCCATGAATAACATAGAAGATAATTGTCCATTTTCCTGGGATATGCTTGATGTCATTTCCAGAGATCTAGATTTCGATGACCTCTTTCAATTTTCTTGCGTGTGCAAGAATTGGAGAGAGTTTCATAAAACTTACTGGAAAAAATTCTTGGCATCCCAAGAACCATTAGTTATTAAAAAGTCTTACCATGATAAGAAATTCTTTTCCTTCATCAGCATAGCTGACCGAAAAGTTTATCACTCGAAGACGATCAATCAATTCTGGTACTTTGCCTATTTTGGATGTTCTAGCGGATATTTGATCATGAAAGGCGACAATAATTCTTTTCTGCTATTAAATCCATTTACAAGAAGAAAGAAGGTAATCAATACATCAACCTTTAAAGTCAAATCTACATATTTTGCTTACCATGTCTTACTTGCTTTTGGCAAAGGCTCGGAGGAATTTGTCTTAGTCACTTCATGTAAAAGTTCTGGCAGTTTGTATGTCTACCAGTCTCGAAATTTGGGTTGGGTTACATATTTAACAATGGGAAATCCATGGAAGGTTGTTGACTTTGTTGTGTTGCATAATACTATATATGTTGTCACCAACGAGGCCATCATAGGTGTACTCGACTTGAATTCCTCAAATATAAATTTTTTAGAAATGAAGAGTATGCCCGATGTAACATCTACGTCACACATAAGGTTGGTTTGCTGTGATGGACAACTTTTAGTGGTTCATATTGAGTCCGAGGAAATATTGAATGTTTACAACGTAGACTTCTCAACCATGAATTATGTCAAGCTGGAAATTTTGGGTGACATTGCCTTATTTTATGCTTCGGGGGAATACTTCTATGCATTAAGCAACCCGAGGAGGTGGGGTTTTGAAAGCAACTCTTTGTATGCCATCAATCTTTCATCCACAACATGTAAAGTGTGTTTAGGGGATGGTAACAGACTGCCAAAATACATTAAGCATGATAGAACTGGAGTTCCTCCAAAGGAAAATGCCTACTTGCTGGATTGGTGCTTTAGACATCAACAATATGAGGTGGATTTTTCTCCAGATGAGTAA

Protein sequence

>Msa0392690

MKNLSLDDALGLISALKCKFGYEKREKYESFLQIMKDYKAERIDARVVKLRVYELLDGHEDLILRFNTFLPTKYEIKLPLDRDDDNEEQDGRMTKTNIKVLKKYRITPSVQLDTGEAMNNIEDNCPFSWDMLDVISRDLDFDDLFQFSCVCKNWREFHKTYWKKFLASQEPLVIKKSYHDKKFFSFISIADRKVYHSKTINQFWYFAYFGCSSGYLIMKGDNNSFLLLNPFTRRKKVINTSTFKVKSTYFAYHVLLAFGKGSEEFVLVTSCKSSGSLYVYQSRNLGWVTYLTMGNPWKVVDFVVLHNTIYVVTNEAIIGVLDLNSSNINFLEMKSMPDVTSTSHIRLVCCDGQLLVVHIESEEILNVYNVDFSTMNYVKLEILGDIALFYASGEYFYALSNPRRWGFESNSLYAINLSSTTCKVCLGDGNRLPKYIKHDRTGVPPKENAYLLDWCFRHQQYEVDFSPDE*