Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0392720 | GAU38899.1 | 48.462 | 130 | 38 | 3 | 4 | 104 | 2 | 131 | 1.30e-25 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0392720 | sp|Q9FKI4|NAA10_ARATH | 40.741 | 135 | 41 | 3 | 4 | 104 | 2 | 131 | 2.04e-22 | 89.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0392720 | A0A2Z6P5E3 | 48.462 | 130 | 38 | 3 | 4 | 104 | 2 | 131 | 6.22e-26 | 104 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0392720 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0392720 | MtrunA17_Chr3g0090311 | 47.692 | 130 | 39 | 3 | 4 | 104 | 2 | 131 | 1.01e-28 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0392720 | AT5G13780.1 | 40.741 | 135 | 41 | 3 | 4 | 104 | 2 | 131 | 2.08e-23 | 89.4 |
Find 27 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCTGTTCCATGTCGTTT+TGG | 0.275526 | 3_2:-5983611 | None:intergenic |
| TCTAAGATGTACAAACTCTT+TGG | 0.380946 | 3_2:+5983302 | Msa0392720:CDS |
| AGTACGCCTTAGTGCATATC+AGG | 0.403838 | 3_2:+5984445 | Msa0392720:CDS |
| CTCCCAAAACGACATGGAAC+AGG | 0.433249 | 3_2:+5983608 | Msa0392720:CDS |
| ACCTTGGGATACAAGATTCA+TGG | 0.434690 | 3_2:+5984504 | Msa0392720:CDS |
| GGTCGTGAGAAGAGGGCAAC+AGG | 0.452975 | 3_2:-5983332 | None:intergenic |
| TTGTGTTTGACACAGATGTT+TGG | 0.455625 | 3_2:+5984417 | Msa0392720:intron |
| CACGTCGCAGAAGATATTAA+TGG | 0.477151 | 3_2:+5983365 | Msa0392720:CDS |
| TCATTTGTTCAAAGAGACCT+TGG | 0.484837 | 3_2:+5984488 | Msa0392720:CDS |
| TGAACAAATGAAAAGCTGCT+TGG | 0.499101 | 3_2:-5984476 | None:intergenic |
| CATTTGTTCAAAGAGACCTT+GGG | 0.501068 | 3_2:+5984489 | Msa0392720:CDS |
| TCTCTGATGGGTTGGGAGAA+CGG | 0.508987 | 3_2:-5983556 | None:intergenic |
| TACCACGTCTCTGATGGGTT+GGG | 0.526888 | 3_2:-5983563 | None:intergenic |
| TAGCGATACCACGTCTCTGA+TGG | 0.539259 | 3_2:-5983569 | None:intergenic |
| ATACCACGTCTCTGATGGGT+TGG | 0.540598 | 3_2:-5983564 | None:intergenic |
| ATGGGTTGGGAGAACGGCGA+GGG | 0.570608 | 3_2:-5983550 | None:intergenic |
| AAGTTGAGGTCGTGAGAAGA+GGG | 0.584002 | 3_2:-5983339 | None:intergenic |
| AGCGATACCACGTCTCTGAT+GGG | 0.588160 | 3_2:-5983568 | None:intergenic |
| TAAGTTGAGGTCGTGAGAAG+AGG | 0.591663 | 3_2:-5983340 | None:intergenic |
| GATTGCCTCCCAAAACGACA+TGG | 0.596635 | 3_2:+5983602 | Msa0392720:CDS |
| GCTCTTCCTGATATGCACTA+AGG | 0.602444 | 3_2:-5984451 | None:intergenic |
| ACCATGAATCTTGTATCCCA+AGG | 0.610002 | 3_2:-5984505 | None:intergenic |
| GATGGGTTGGGAGAACGGCG+AGG | 0.612867 | 3_2:-5983551 | None:intergenic |
| AGAGTTTGTACATCTTAGAG+AGG | 0.637261 | 3_2:-5983298 | None:intergenic |
| CTCCCAACCCATCAGAGACG+TGG | 0.645269 | 3_2:+5983561 | Msa0392720:CDS |
| TCTGCGACGTGAATAAGTTG+AGG | 0.654590 | 3_2:-5983353 | None:intergenic |
| ACATCTTAGAGAGGTCGCAA+AGG | 0.660670 | 3_2:-5983289 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATATGTATCTTTTATATT+TGG | + | chr3_2:5984077-5984096 | Msa0392720:intron | 10.0% |
| !!! | TAATATGTATCTTTTATATT+TGG | + | chr3_2:5984077-5984096 | Msa0392720:intron | 10.0% |
| !! | AAAATGATCCTTAATTATTT+CGG | - | chr3_2:5984016-5984035 | None:intergenic | 15.0% |
| !! | TTCAAGAAAATTATTTGAAT+AGG | - | chr3_2:5984226-5984245 | None:intergenic | 15.0% |
| !! | AAAATGATCCTTAATTATTT+CGG | - | chr3_2:5984016-5984035 | None:intergenic | 15.0% |
| !! | TTCAAGAAAATTATTTGAAT+AGG | - | chr3_2:5984226-5984245 | None:intergenic | 15.0% |
| !!! | ATTTTTAGCCGAAATAATTA+AGG | + | chr3_2:5984005-5984024 | Msa0392720:intron | 20.0% |
| !!! | ATTTTTAGCCGAAATAATTA+AGG | + | chr3_2:5984005-5984024 | Msa0392720:intron | 20.0% |
| !!! | TTGTTACAGTTTTGTTAGTT+AGG | + | chr3_2:5983943-5983962 | Msa0392720:intron | 25.0% |
| !!! | GAGTTTTAATCCAATCTAAT+TGG | - | chr3_2:5983970-5983989 | None:intergenic | 25.0% |
| !!! | TTGTTACAGTTTTGTTAGTT+AGG | + | chr3_2:5983943-5983962 | Msa0392720:intron | 25.0% |
| !!! | GAGTTTTAATCCAATCTAAT+TGG | - | chr3_2:5983970-5983989 | None:intergenic | 25.0% |
| ! | TCTAAGATGTACAAACTCTT+TGG | + | chr3_2:5983302-5983321 | Msa0392720:CDS | 30.0% |
| ATTCAAGAGGAAATTAGTGA+AGG | + | chr3_2:5983516-5983535 | Msa0392720:intron | 30.0% | |
| TATTAATTCAAATTGCACCG+AGG | + | chr3_2:5983698-5983717 | Msa0392720:intron | 30.0% | |
| ATTAATTCAAATTGCACCGA+GGG | + | chr3_2:5983699-5983718 | Msa0392720:intron | 30.0% | |
| ATCAAATTAGTCAATGCATC+TGG | + | chr3_2:5983914-5983933 | Msa0392720:intron | 30.0% | |
| TTAGTTAGGTCCAATTAGAT+TGG | + | chr3_2:5983957-5983976 | Msa0392720:intron | 30.0% | |
| GAGTCAAGTTATTAACTACT+TGG | + | chr3_2:5984111-5984130 | Msa0392720:intron | 30.0% | |
| ATATGTAGAATTGCAGAACT+AGG | - | chr3_2:5984265-5984284 | None:intergenic | 30.0% | |
| TTGTATTTGACACAGATGTT+TGG | + | chr3_2:5984372-5984391 | Msa0392720:intron | 30.0% | |
| ! | TCTAAGATGTACAAACTCTT+TGG | + | chr3_2:5983302-5983321 | Msa0392720:CDS | 30.0% |
| ATTCAAGAGGAAATTAGTGA+AGG | + | chr3_2:5983516-5983535 | Msa0392720:intron | 30.0% | |
| TATTAATTCAAATTGCACCG+AGG | + | chr3_2:5983698-5983717 | Msa0392720:intron | 30.0% | |
| ATTAATTCAAATTGCACCGA+GGG | + | chr3_2:5983699-5983718 | Msa0392720:intron | 30.0% | |
| ATCAAATTAGTCAATGCATC+TGG | + | chr3_2:5983914-5983933 | Msa0392720:intron | 30.0% | |
| TTAGTTAGGTCCAATTAGAT+TGG | + | chr3_2:5983957-5983976 | Msa0392720:intron | 30.0% | |
| GAGTCAAGTTATTAACTACT+TGG | + | chr3_2:5984111-5984130 | Msa0392720:intron | 30.0% | |
| ATATGTAGAATTGCAGAACT+AGG | - | chr3_2:5984265-5984284 | None:intergenic | 30.0% | |
| TTGTATTTGACACAGATGTT+TGG | + | chr3_2:5984372-5984391 | Msa0392720:intron | 30.0% | |
| AGAGTTTGTACATCTTAGAG+AGG | - | chr3_2:5983301-5983320 | None:intergenic | 35.0% | |
| TTTCCTCTTGAATTTCACAG+TGG | - | chr3_2:5983509-5983528 | None:intergenic | 35.0% | |
| ! | CTCAGTTTCTGAGAGTTATA+AGG | + | chr3_2:5983729-5983748 | Msa0392720:intron | 35.0% |
| AAGTGTGTCTTCTTGTAACA+GGG | + | chr3_2:5984318-5984337 | Msa0392720:intron | 35.0% | |
| TTGTGTTTGACACAGATGTT+TGG | + | chr3_2:5984417-5984436 | Msa0392720:intron | 35.0% | |
| TGAACAAATGAAAAGCTGCT+TGG | - | chr3_2:5984479-5984498 | None:intergenic | 35.0% | |
| TCATTTGTTCAAAGAGACCT+TGG | + | chr3_2:5984488-5984507 | Msa0392720:CDS | 35.0% | |
| CATTTGTTCAAAGAGACCTT+GGG | + | chr3_2:5984489-5984508 | Msa0392720:CDS | 35.0% | |
| AGAGTTTGTACATCTTAGAG+AGG | - | chr3_2:5983301-5983320 | None:intergenic | 35.0% | |
| TTTCCTCTTGAATTTCACAG+TGG | - | chr3_2:5983509-5983528 | None:intergenic | 35.0% | |
| ! | CTCAGTTTCTGAGAGTTATA+AGG | + | chr3_2:5983729-5983748 | Msa0392720:intron | 35.0% |
| AAGTGTGTCTTCTTGTAACA+GGG | + | chr3_2:5984318-5984337 | Msa0392720:intron | 35.0% | |
| TTGTGTTTGACACAGATGTT+TGG | + | chr3_2:5984417-5984436 | Msa0392720:intron | 35.0% | |
| TGAACAAATGAAAAGCTGCT+TGG | - | chr3_2:5984479-5984498 | None:intergenic | 35.0% | |
| TCATTTGTTCAAAGAGACCT+TGG | + | chr3_2:5984488-5984507 | Msa0392720:CDS | 35.0% | |
| CATTTGTTCAAAGAGACCTT+GGG | + | chr3_2:5984489-5984508 | Msa0392720:CDS | 35.0% | |
| CACGTCGCAGAAGATATTAA+TGG | + | chr3_2:5983365-5983384 | Msa0392720:CDS | 40.0% | |
| !!! | AACCTGTTCCATGTCGTTTT+GGG | - | chr3_2:5983613-5983632 | None:intergenic | 40.0% |
| !! | AAACCTGTTCCATGTCGTTT+TGG | - | chr3_2:5983614-5983633 | None:intergenic | 40.0% |
| GAAGTGTGTCTTCTTGTAAC+AGG | + | chr3_2:5984317-5984336 | Msa0392720:intron | 40.0% | |
| CACGTCGCAGAAGATATTAA+TGG | + | chr3_2:5983365-5983384 | Msa0392720:CDS | 40.0% | |
| !!! | AACCTGTTCCATGTCGTTTT+GGG | - | chr3_2:5983613-5983632 | None:intergenic | 40.0% |
| !! | AAACCTGTTCCATGTCGTTT+TGG | - | chr3_2:5983614-5983633 | None:intergenic | 40.0% |
| GAAGTGTGTCTTCTTGTAAC+AGG | + | chr3_2:5984317-5984336 | Msa0392720:intron | 40.0% | |
| ACATCTTAGAGAGGTCGCAA+AGG | - | chr3_2:5983292-5983311 | None:intergenic | 45.0% | |
| AAGTTGAGGTCGTGAGAAGA+GGG | - | chr3_2:5983342-5983361 | None:intergenic | 45.0% | |
| ! | TAAGTTGAGGTCGTGAGAAG+AGG | - | chr3_2:5983343-5983362 | None:intergenic | 45.0% |
| TCTGCGACGTGAATAAGTTG+AGG | - | chr3_2:5983356-5983375 | None:intergenic | 45.0% | |
| GAAACTGAGTTCGAATCCCT+CGG | - | chr3_2:5983718-5983737 | None:intergenic | 45.0% | |
| AGTACGCCTTAGTGCATATC+AGG | + | chr3_2:5984445-5984464 | Msa0392720:CDS | 45.0% | |
| GCTCTTCCTGATATGCACTA+AGG | - | chr3_2:5984454-5984473 | None:intergenic | 45.0% | |
| ACATCTTAGAGAGGTCGCAA+AGG | - | chr3_2:5983292-5983311 | None:intergenic | 45.0% | |
| AAGTTGAGGTCGTGAGAAGA+GGG | - | chr3_2:5983342-5983361 | None:intergenic | 45.0% | |
| ! | TAAGTTGAGGTCGTGAGAAG+AGG | - | chr3_2:5983343-5983362 | None:intergenic | 45.0% |
| TCTGCGACGTGAATAAGTTG+AGG | - | chr3_2:5983356-5983375 | None:intergenic | 45.0% | |
| GAAACTGAGTTCGAATCCCT+CGG | - | chr3_2:5983718-5983737 | None:intergenic | 45.0% | |
| AGTACGCCTTAGTGCATATC+AGG | + | chr3_2:5984445-5984464 | Msa0392720:CDS | 45.0% | |
| GCTCTTCCTGATATGCACTA+AGG | - | chr3_2:5984454-5984473 | None:intergenic | 45.0% | |
| !! | TCTCTGATGGGTTGGGAGAA+CGG | - | chr3_2:5983559-5983578 | None:intergenic | 50.0% |
| !! | TACCACGTCTCTGATGGGTT+GGG | - | chr3_2:5983566-5983585 | None:intergenic | 50.0% |
| ! | ATACCACGTCTCTGATGGGT+TGG | - | chr3_2:5983567-5983586 | None:intergenic | 50.0% |
| AGCGATACCACGTCTCTGAT+GGG | - | chr3_2:5983571-5983590 | None:intergenic | 50.0% | |
| TAGCGATACCACGTCTCTGA+TGG | - | chr3_2:5983572-5983591 | None:intergenic | 50.0% | |
| GATTGCCTCCCAAAACGACA+TGG | + | chr3_2:5983602-5983621 | Msa0392720:CDS | 50.0% | |
| !!! | CTGTTCCATGTCGTTTTGGG+AGG | - | chr3_2:5983610-5983629 | None:intergenic | 50.0% |
| CTCCCAAAACGACATGGAAC+AGG | + | chr3_2:5983608-5983627 | Msa0392720:CDS | 50.0% | |
| !! | TCTCTGATGGGTTGGGAGAA+CGG | - | chr3_2:5983559-5983578 | None:intergenic | 50.0% |
| !! | TACCACGTCTCTGATGGGTT+GGG | - | chr3_2:5983566-5983585 | None:intergenic | 50.0% |
| ! | ATACCACGTCTCTGATGGGT+TGG | - | chr3_2:5983567-5983586 | None:intergenic | 50.0% |
| AGCGATACCACGTCTCTGAT+GGG | - | chr3_2:5983571-5983590 | None:intergenic | 50.0% | |
| TAGCGATACCACGTCTCTGA+TGG | - | chr3_2:5983572-5983591 | None:intergenic | 50.0% | |
| GATTGCCTCCCAAAACGACA+TGG | + | chr3_2:5983602-5983621 | Msa0392720:CDS | 50.0% | |
| !!! | CTGTTCCATGTCGTTTTGGG+AGG | - | chr3_2:5983610-5983629 | None:intergenic | 50.0% |
| CTCCCAAAACGACATGGAAC+AGG | + | chr3_2:5983608-5983627 | Msa0392720:CDS | 50.0% | |
| GGTCGTGAGAAGAGGGCAAC+AGG | - | chr3_2:5983335-5983354 | None:intergenic | 60.0% | |
| !! | ATGGGTTGGGAGAACGGCGA+GGG | - | chr3_2:5983553-5983572 | None:intergenic | 60.0% |
| CTCCCAACCCATCAGAGACG+TGG | + | chr3_2:5983561-5983580 | Msa0392720:CDS | 60.0% | |
| GGTCGTGAGAAGAGGGCAAC+AGG | - | chr3_2:5983335-5983354 | None:intergenic | 60.0% | |
| !! | ATGGGTTGGGAGAACGGCGA+GGG | - | chr3_2:5983553-5983572 | None:intergenic | 60.0% |
| CTCCCAACCCATCAGAGACG+TGG | + | chr3_2:5983561-5983580 | Msa0392720:CDS | 60.0% | |
| !! | GATGGGTTGGGAGAACGGCG+AGG | - | chr3_2:5983554-5983573 | None:intergenic | 65.0% |
| !! | GATGGGTTGGGAGAACGGCG+AGG | - | chr3_2:5983554-5983573 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 5983260 | 5984524 | 5983260 | ID=Msa0392720;Name=Msa0392720 |
| chr3_2 | mRNA | 5983260 | 5984524 | 5983260 | ID=Msa0392720-mRNA-1;Parent=Msa0392720;Name=Msa0392720-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|1|0.5|0.33|3|0|104 |
| chr3_2 | exon | 5983260 | 5983386 | 5983260 | ID=Msa0392720-mRNA-1:exon:1515;Parent=Msa0392720-mRNA-1 |
| chr3_2 | exon | 5983538 | 5983629 | 5983538 | ID=Msa0392720-mRNA-1:exon:1516;Parent=Msa0392720-mRNA-1 |
| chr3_2 | exon | 5984432 | 5984524 | 5984432 | ID=Msa0392720-mRNA-1:exon:1517;Parent=Msa0392720-mRNA-1 |
| chr3_2 | CDS | 5983260 | 5983386 | 5983260 | ID=Msa0392720-mRNA-1:cds;Parent=Msa0392720-mRNA-1 |
| chr3_2 | CDS | 5983538 | 5983629 | 5983538 | ID=Msa0392720-mRNA-1:cds;Parent=Msa0392720-mRNA-1 |
| chr3_2 | CDS | 5984432 | 5984524 | 5984432 | ID=Msa0392720-mRNA-1:cds;Parent=Msa0392720-mRNA-1 |
| Gene Sequence |
| Protein sequence |
