AlfalfaGEDB | Msa0392980

M. sativa cultivar zhongmu-4 / Msa0392980

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0392980	XP_039685680.1	84.731	799	102	3	1	779	1	799	0.0	1388

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0392980	sp\|Q9SZL8\|FRS5_ARATH	27.925	641	382	14	32	665	70	637	1.25e-69	248

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0392980	D3YBB9	76.332	807	174	4	1	798	49	847	0.0	1281

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TFs/TRs:

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Gene ID	Type	Classification
Msa0392980	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0392980	MtrunA17_Chr3g0088371	83.152	184	28	1	618	798	1	184	2.51e-102	313
Msa0392980	MtrunA17_Chr2g0324011	30.709	635	364	12	36	665	8	571	3.18e-101	325
Msa0392980	MtrunA17_Chr3g0082121	31.693	691	384	17	41	727	49	655	1.59e-100	326
Msa0392980	MtrunA17_Chr1g0202341	29.606	635	366	17	34	662	50	609	7.14e-80	270
Msa0392980	MtrunA17_Chr8g0363841	28.776	629	375	13	40	665	22	580	4.82e-75	257
Msa0392980	MtrunA17_Chr1g0203721	26.026	780	452	20	1	739	16	711	6.08e-73	256
Msa0392980	MtrunA17_Chr5g0448761	25.318	707	449	14	31	728	56	692	1.69e-68	241
Msa0392980	MtrunA17_Chr2g0296781	27.688	679	405	20	39	708	1	602	1.82e-68	240
Msa0392980	MtrunA17_Chr6g0458391	26.984	630	357	15	41	662	231	765	2.33e-65	232
Msa0392980	MtrunA17_Chr6g0477261	29.412	527	314	14	180	699	7	482	5.96e-65	229

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0392980	AT4G38180.1	27.925	641	382	14	32	665	70	637	1.27e-70	248
Msa0392980	AT4G15090.1	25.000	696	435	15	50	741	66	678	1.98e-59	217
Msa0392980	AT4G15090.5	25.000	696	435	15	50	741	66	678	1.98e-59	217
Msa0392980	AT4G15090.3	25.000	696	435	15	50	741	66	678	1.98e-59	217
Msa0392980	AT4G15090.2	25.000	696	435	15	50	741	66	678	1.98e-59	217
Msa0392980	AT4G15090.4	25.000	696	435	15	50	741	66	678	1.98e-59	217
Msa0392980	AT2G27110.2	25.070	714	423	19	10	719	29	634	1.21e-53	200
Msa0392980	AT2G27110.1	25.070	714	423	19	10	719	29	634	1.21e-53	200
Msa0392980	AT2G27110.3	27.308	509	313	11	213	719	36	489	4.79e-53	197
Msa0392980	AT3G06250.2	25.118	633	351	16	34	661	189	703	5.09e-52	194

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Find 125 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCTCTGGTTTATGTAA+TGG	0.504865	3_2:-6666104	None:intergenic
AACAAAGAAGGTTTCAAGAA+GGG	0.545537	3_2:+6665906	Msa0392980:CDS
AACAATAAATATCGGCCATT+AGG	0.388497	3_2:+6666506	Msa0392980:CDS
AACTCGTCAAAAGGAGATGG+TGG	0.603142	3_2:+6666298	Msa0392980:CDS
AAGAAAGACCAAATCCAATT+AGG	0.397771	3_2:+6668354	Msa0392980:CDS
AATCAGATTTCGACTACAAT+TGG	0.318540	3_2:+6667274	Msa0392980:CDS
AATCGAACAAGTGAATGGTC+TGG	0.540971	3_2:-6667477	None:intergenic
AATGAAGCTCTTTCGAAACC+AGG	0.496588	3_2:+6666356	Msa0392980:CDS
AATTCTTGATTGTTATGCAT+TGG	0.467731	3_2:+6668592	Msa0392980:three_prime_UTR
ACAAAATAAGAAATCGGATA+AGG	0.469178	3_2:+6665860	Msa0392980:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATATTAACTTCATCTGAGAG+TGG	+	chr3_2:6665654-6665673	Msa0392980:CDS	30.0%
TAAAGAATCATGGAAGTCTT+TGG	+	chr3_2:6665740-6665759	Msa0392980:CDS	30.0%
CGTTATGTTTGTAACAAAGA+AGG	+	chr3_2:6665894-6665913	Msa0392980:CDS	30.0%
TAACAAAGAAGGTTTCAAGA+AGG	+	chr3_2:6665905-6665924	Msa0392980:CDS	30.0%
AACAAAGAAGGTTTCAAGAA+GGG	+	chr3_2:6665906-6665925	Msa0392980:CDS	30.0%
TAAAGAAAAACCAGAGGAAA+GGG	+	chr3_2:6665995-6666014	Msa0392980:CDS	30.0%
AGAACATCTTATGAAGTGAT+GGG	+	chr3_2:6666209-6666228	Msa0392980:CDS	30.0%
TTTAAGTCCGAAAATCGAAA+AGG	-	chr3_2:6666263-6666282	None:intergenic	30.0%
TGGATATCATAGTAAAAACC+TGG	-	chr3_2:6666377-6666396	None:intergenic	30.0%
CAACAAGATTATGCTCTATT+TGG	+	chr3_2:6666449-6666468	Msa0392980:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	6665600	6668633	6665600	ID=Msa0392980;Name=Msa0392980
chr3_2	mRNA	6665600	6668633	6665600	ID=Msa0392980-mRNA-1;Parent=Msa0392980;Name=Msa0392980-mRNA-1;_AED=0.00;_eAED=0.00;_QI=45\|1\|1\|1\|1\|1\|2\|152\|800
chr3_2	exon	6665600	6666527	6665600	ID=Msa0392980-mRNA-1:exon:1647;Parent=Msa0392980-mRNA-1
chr3_2	exon	6666962	6668633	6666962	ID=Msa0392980-mRNA-1:exon:1648;Parent=Msa0392980-mRNA-1
chr3_2	five_prime_UTR	6665600	6665644	6665600	ID=Msa0392980-mRNA-1:five_prime_utr;Parent=Msa0392980-mRNA-1
chr3_2	CDS	6665645	6666527	6665645	ID=Msa0392980-mRNA-1:cds;Parent=Msa0392980-mRNA-1
chr3_2	CDS	6666962	6668481	6666962	ID=Msa0392980-mRNA-1:cds;Parent=Msa0392980-mRNA-1
chr3_2	three_prime_UTR	6668482	6668633	6668482	ID=Msa0392980-mRNA-1:three_prime_utr;Parent=Msa0392980-mRNA-1

Gene Sequence

>Msa0392980

ATGGATTTGATATTAACTTCATCTGAGAGTGGACAATTGACGGTTGAAAATTCAGTTATTGATGCGGATAGTTCTCAATGTTACTCATTTAGTGATAAAGAATCATGGAAGTCTTTGGTCTTTAAATCTATAAAAGAGATTGAAGATTTTTATTTTAATTATGCATATAACACTGGATTCTCTGTTAGGAGTATGTTGAAGTCCAAGAACAATTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGTTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTCTTGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGCGTTGAATGTCTATAAGATATATAAGTGGGAAGTTGATCACTGTCATCCATTACATAAACCAGAGCATTTGTGCTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTCAAGGAGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAGAACTATCTAATGACAACTCGTCAAAAGGAGATGGTGGCTGGTGAAGCAACTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTTTCGAAACCAGGTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCCAGTATTTTTTGGGCAGATGGAATTATGCAACAAGATTATGCTCTATTTGGTGATGTTGTCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGCTGCCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTGGTGCGTTGTTGAAGTCAATTCCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGCAAAGAAAAATCTCGGCTCTCGTGCAAACAGTGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAACTGTTTTAATGGAAGGCCTACTTCGGACTTTAAGTGGCTTGTTCAAACTCATTCAAATCGTATGCATTGGTCTTCAGTTTGGGTGAGGTCACATTTTACAGCTGGTTTGAAAACGACACAGCTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACTTGTTCGATTCTTTAGTCATTTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGAGTTGGACTTTAAGGCTGCAAACACTAGAACAAAAAATAATTATCCCAACAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACACAACAAAAGGGTCAGAATTGAACAAGTTCTTCAAAGTTTTCACAATTGAGAAGGTTGATTATAATTACGATGATGTTGATAATGATAATGAGAGGGTTTCGAATGTTGATGTTTTGGATGCAGAATTTGAAGAAAACCTTTCCCCAAGGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTGGGAGCAAAAGTTTTACTTGTTCATGTCGTATGTTTGAGAACCGGGGTTTCTTATGTCGACATGTTTTCAAGATTTTGGAGGTCTTAGGTGGTCATGTGCAATATCATTTTTTGAAGACTATACCTGCACAATATGTTTTAAAGCGGTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCCACCATGAATGTTGGCACTAAAGACACCACTGAAGCGCAACGATATCAACAAATTTGTGCTGTTACAGTTCAGCTTTCTACACGTGTTTGCGCAGATGCGGAGGCATCTGAAATATTCCTCAACGGTGTTCTTGAGGCAGGGAAAAAGGCAGAGGAGTTTCTTATTTCTAAAGGTATTCATACAGATCGATCATCCGTTACGCCTTCTAAGTCTTCAAAAGCAGTTGATGTCTGTGAACCATCTGTTGGGACTGCACCAAAGTTCAAAGAAAGACCAAATCCAATTAGGTCAAAGAAGCGACTTAAAAGTGATTATGAAAAAGCTAGAGCAAGACAGAAATTCCTTATAGCTCGGAAGAAGCAAAAGAAAGATGACGAGGCTTTAACAAATTAA

Protein sequence

>Msa0392980

MDLILTSSESGQLTVENSVIDADSSQCYSFSDKESWKSLVFKSIKEIEDFYFNYAYNTGFSVRSMLKSKNNSQNKKSDKVHYVRYVCNKEGFKKGSLLNPKNRPTCDSPVLVEYEKAKEKPEERVGCKAGIYLKLDEALNVYKIYKWEVDHCHPLHKPEHLCYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGQGVGGTENLPFRFSDLKNYLMTTRQKEMVAGEATVIQEFFRNEALSKPGFYYDIQVDAAEDIASIFWADGIMQQDYALFGDVVSFDTTYRTNNKYRPLAAFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQAGALLKSIPNVFQGVFHGLCSWHMGENAKKNLGSRANSAFFDELTNLVSNVEDESDFDYNWDQMMKNCFNGRPTSDFKWLVQTHSNRMHWSSVWVRSHFTAGLKTTQLSESFNAFLRGFLQPDHSLVRFFSHFNIMVQRMRDNHAELDFKAANTRTKNNYPNSQLMRSVVNKYTPSCFSFIHSQYDLSFKYYYEEDTTKGSELNKFFKVFTIEKVDYNYDDVDNDNERVSNVDVLDAEFEENLSPRFEDHNRLDERVVTVDIGSKSFTCSCRMFENRGFLCRHVFKILEVLGGHVQYHFLKTIPAQYVLKRWTRDVRPSVDKLKSTMNVGTKDTTEAQRYQQICAVTVQLSTRVCADAEASEIFLNGVLEAGKKAEEFLISKGIHTDRSSVTPSKSSKAVDVCEPSVGTAPKFKERPNPIRSKKRLKSDYEKARARQKFLIARKKQKKDDEALTN*