AlfalfaGEDB | Msa0393400

M. sativa cultivar zhongmu-4 / Msa0393400

KEGG: K04730
GO: -
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0393400	KEH40526.1	52.166	554	172	14	33	503	37	580	3.09e-161	480

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0393400	sp\|Q1MX30\|XA21_ORYSI	38.307	449	245	12	89	511	570	1012	1.50e-75	260
Msa0393400	sp\|Q1MX30\|XA21_ORYSI	39.189	148	80	2	98	235	283	430	1.54e-15	83.6
Msa0393400	sp\|Q1MX30\|XA21_ORYSI	30.877	285	154	8	35	296	33	297	1.34e-11	70.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0393400	A0A072VFZ7	52.166	554	172	14	33	503	37	580	1.48e-161	480

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0393400	MtrunA17_Chr7g0248261	82.079	279	47	3	236	512	683	960	1.75e-158	475
Msa0393400	MtrunA17_Chr7g0248261	51.648	182	57	1	84	234	233	414	7.07e-41	157
Msa0393400	MtrunA17_Chr1g0159871	58.667	450	154	8	89	512	493	936	4.19e-155	466
Msa0393400	MtrunA17_Chr1g0159871	52.151	186	55	2	80	234	190	372	6.55e-44	166
Msa0393400	MtrunA17_Chr5g0436861	55.973	452	165	8	89	512	564	1009	1.93e-145	442
Msa0393400	MtrunA17_Chr5g0436861	52.874	174	51	1	92	234	270	443	6.54e-42	160
Msa0393400	MtrunA17_Chr5g0436861	38.462	234	118	4	16	232	17	241	1.88e-35	141
Msa0393400	MtrunA17_Chr5g0436841	77.698	278	55	3	236	512	779	1050	4.69e-144	440
Msa0393400	MtrunA17_Chr5g0436841	52.601	173	51	1	93	234	320	492	3.04e-40	155
Msa0393400	MtrunA17_Chr5g0436841	44.019	209	93	5	32	234	78	268	4.31e-30	125

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0393400	AT5G39390.1	35.977	517	266	15	19	510	21	497	5.69e-79	256
Msa0393400	AT3G47570.1	47.143	280	137	4	235	509	727	1000	3.92e-74	253
Msa0393400	AT3G47570.1	35.577	208	107	7	32	232	23	210	8.74e-21	97.1
Msa0393400	AT3G47570.1	37.671	146	83	2	93	231	264	408	8.18e-19	90.9
Msa0393400	AT3G47570.1	35.252	139	78	3	99	231	372	504	7.42e-14	75.1
Msa0393400	AT3G47580.1	34.560	489	247	14	85	510	524	1002	1.56e-73	251
Msa0393400	AT3G47580.1	30.000	220	131	4	32	234	23	236	4.59e-20	94.7
Msa0393400	AT3G47580.1	38.235	136	77	1	97	225	268	403	1.52e-14	77.4
Msa0393400	AT3G47090.1	43.972	282	145	5	235	510	726	1000	3.05e-70	243
Msa0393400	AT3G47090.1	40.972	144	78	1	98	234	269	412	2.74e-19	92.0

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Find 103 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGTTGCATGATATTGA+AGG	0.553882	3_2:+7495394	Msa0393400:CDS
AAATGGCAAGGGGTTAAATG+CGG	0.728518	3_2:+7494278	Msa0393400:CDS
AAATGTTGCATGATATTGAA+GGG	0.589773	3_2:+7495395	Msa0393400:CDS
AACGTTGGTCCACTATCTCA+AGG	0.587667	3_2:-7496026	None:intergenic
AAGCTGTAGATATCTCCCTG+TGG	0.756753	3_2:-7495895	None:intergenic
AAGTGAATCATCGATGGAAA+TGG	0.405835	3_2:+7493898	None:intergenic
AATTACAATTTATATGTATT+GGG	0.288707	3_2:+7496324	Msa0393400:three_prime_UTR
ACAACTGGTCAAACTTACTG+TGG	0.682560	3_2:-7496263	None:intergenic
ACAATAGGATATATTCCTCC+TGG	0.557165	3_2:+7495658	Msa0393400:CDS
ACAATCTCAACCTCAAACAA+AGG	0.509726	3_2:+7495428	Msa0393400:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGAAATTAAAACGAAAGGT+TGG	+	chr3_2:7494039-7494058	Msa0393400:intron	30.0%
AAATCATAGTAAAGACCATG+TGG	-	chr3_2:7494067-7494086	None:intergenic	30.0%
TTGAAATGTTAGATTTCCCA+AGG	-	chr3_2:7494372-7494391	None:intergenic	30.0%
GTTGAAGATAACCTATTAAC+TGG	+	chr3_2:7494475-7494494	Msa0393400:CDS	30.0%
TGCAAGTGTTTGATATATCA+TGG	+	chr3_2:7494533-7494552	Msa0393400:CDS	30.0%
GTCGATTAAATAAACTTGAG+CGG	+	chr3_2:7494590-7494609	Msa0393400:CDS	30.0%
GTGAAGGCTAATATAATACA+TGG	+	chr3_2:7494784-7494803	Msa0393400:intron	30.0%
GGTGCATCTTACTTAAATAT+TGG	+	chr3_2:7494838-7494857	Msa0393400:intron	30.0%
GGAATGATTCCAAATTCAAT+CGG	+	chr3_2:7494877-7494896	Msa0393400:CDS	30.0%
TTAAGCTTTCCGATTGAATT+TGG	-	chr3_2:7494889-7494908	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	7493911	7496378	7493911	ID=Msa0393400;Name=Msa0393400
chr3_2	mRNA	7493911	7496378	7493911	ID=Msa0393400-mRNA-1;Parent=Msa0393400;Name=Msa0393400-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0\|0.2\|0.16\|0.83\|1\|1\|6\|154\|512
chr3_2	exon	7493911	7494004	7493911	ID=Msa0393400-mRNA-1:exon:1899;Parent=Msa0393400-mRNA-1
chr3_2	exon	7494183	7494353	7494183	ID=Msa0393400-mRNA-1:exon:1900;Parent=Msa0393400-mRNA-1
chr3_2	exon	7494458	7494783	7494458	ID=Msa0393400-mRNA-1:exon:1901;Parent=Msa0393400-mRNA-1
chr3_2	exon	7494877	7494986	7494877	ID=Msa0393400-mRNA-1:exon:1902;Parent=Msa0393400-mRNA-1
chr3_2	exon	7495201	7495679	7495201	ID=Msa0393400-mRNA-1:exon:1903;Parent=Msa0393400-mRNA-1
chr3_2	exon	7495866	7496378	7495866	ID=Msa0393400-mRNA-1:exon:1904;Parent=Msa0393400-mRNA-1
chr3_2	CDS	7493911	7494004	7493911	ID=Msa0393400-mRNA-1:cds;Parent=Msa0393400-mRNA-1
chr3_2	CDS	7494183	7494353	7494183	ID=Msa0393400-mRNA-1:cds;Parent=Msa0393400-mRNA-1
chr3_2	CDS	7494458	7494783	7494458	ID=Msa0393400-mRNA-1:cds;Parent=Msa0393400-mRNA-1
chr3_2	CDS	7494877	7494986	7494877	ID=Msa0393400-mRNA-1:cds;Parent=Msa0393400-mRNA-1
chr3_2	CDS	7495201	7495679	7495201	ID=Msa0393400-mRNA-1:cds;Parent=Msa0393400-mRNA-1
chr3_2	CDS	7495866	7496224	7495866	ID=Msa0393400-mRNA-1:cds;Parent=Msa0393400-mRNA-1
chr3_2	three_prime_UTR	7496225	7496378	7496225	ID=Msa0393400-mRNA-1:three_prime_utr;Parent=Msa0393400-mRNA-1

Gene Sequence

>Msa0393400

ATGGAAATGGATCCTAGCATCATGTCAAAGGTTGGACCAACATGTCTTTATTACGTGCTGCACCAATATAGGCATTCACCGAAGGATAAAATAGACACCGACAAGCTAGCTTTGCTTGCTTTGAAGAAAAAACTTACAAATGACGTGCCTGATTCTCTGCCGTCATGGAATGAGTCTCTACATTTTTGTAAATGGCAAGGGGTTAAATGCGGTCGTGCTCACATGAGAGTCTCTATCTTGCATTTGGAGAATCAAACCTGGGGTGCGGTTGAAGGATTCTATGTTGAAGATAACCTATTAACTGGAATCTTTCCGTTTTCAATTTCCAACCTCACTGAATTGCAAGTGTTTGATATATCATGGAATGCTTTTAATGGCCCAATACCTCTTACTTTGGGTCGATTAAATAAACTTGAGCGGTTTCATATTAGCGCTAATAATTTTGGGAGTGGAGGAGATCATGATTTGGACTTTCTTTCCTGGTTAACCAACTGCAGTCAATTGTCAGCACTCATTTTTAGTAAAAATGGATTTGGTGGTATGTTGCCGAATGTTATAGGAAATTTTTCTTTCCGTCTCAGTTGGCTTAATGGAATGATTCCAAATTCAATCGGAAAGCTTAAGAATCTAGTACGATTGGTCTTGCAATCAAACAAATTACATGGTGTCATTCCTGATAGTATTGGAAATCTCACTATCTTACTTATTGCTGTAAAGGTCTTGAATCTTGAAACACGCGGAGCAGCAAAGACTTTCACAGCGGAATGCAAAGCTCTAGGAAAGATGAGGCATTGGAATCTTGTAAGAATCCTAACTTGTTGTTCAAGTATTGATTACAAAGGTGAAGATTTCAAGGCTATTGTTTTTGAGTTCATGCCTAACGGGAGTCTAGAAAAAATGTTGCATGATATTGAAGGGTCTGAAAATCACAATCTCAACCTCAAACAAAGGGTAGATTTTGCTCTTGATATAGCTCATGCACTTGATTATCTTCACAATGATACAGAGCAAATTACAGTTCACTGCGATGTTAAGCCAAGCAATGTTCTTGATGATGACAATGTAGCCCACTTGGGAGACTTTGGTTTAGCTAGGATCATTCTTGGAGCCACCGAGCATTCAAGTAAAGATCAAGTAATTTCCTCTAGAATTAAAGGAACAATAGGATATATTCCTCCTGAGTACGGAACAGGTGTTCCAGTATCACCACCACAGGGAGATATCTACAGCTTTGGGATTCTTCTGTTAGAGATGCTCACCAGAAAGAGACCAACCGATAGTATGTTTTGTGAGAATTTAAGCCTACACAAGTTTTGTAAAATGAAAATTTCTGAAGGAATCCTTGAGATAGTGGACCAACGTTTGCTTATGCCGTTTGTCGAAGATCAGACAGGAATTGTGGAAAACAAGATTAAAAAGTGTTTGGTGATGTTTGGTAGAATTGGAATTGCATGTTCTGAAGAGTTTCCTGCTCATCGAATGCTGATAAAAGATGTTATTGATAAGTTGAATGAAATCAAATCAAAGATTTCATGCTAG

Protein sequence

>Msa0393400

MEMDPSIMSKVGPTCLYYVLHQYRHSPKDKIDTDKLALLALKKKLTNDVPDSLPSWNESLHFCKWQGVKCGRAHMRVSILHLENQTWGAVEGFYVEDNLLTGIFPFSISNLTELQVFDISWNAFNGPIPLTLGRLNKLERFHISANNFGSGGDHDLDFLSWLTNCSQLSALIFSKNGFGGMLPNVIGNFSFRLSWLNGMIPNSIGKLKNLVRLVLQSNKLHGVIPDSIGNLTILLIAVKVLNLETRGAAKTFTAECKALGKMRHWNLVRILTCCSSIDYKGEDFKAIVFEFMPNGSLEKMLHDIEGSENHNLNLKQRVDFALDIAHALDYLHNDTEQITVHCDVKPSNVLDDDNVAHLGDFGLARIILGATEHSSKDQVISSRIKGTIGYIPPEYGTGVPVSPPQGDIYSFGILLLEMLTRKRPTDSMFCENLSLHKFCKMKISEGILEIVDQRLLMPFVEDQTGIVENKIKKCLVMFGRIGIACSEEFPAHRMLIKDVIDKLNEIKSKISC*