Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393640 | XP_003598191.1 | 85.443 | 158 | 23 | 0 | 1 | 158 | 118 | 275 | 9.73e-93 | 280 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393640 | G7IVM3 | 85.443 | 158 | 23 | 0 | 1 | 158 | 118 | 275 | 4.65e-93 | 280 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0393640 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393640 | MtrunA17_Chr3g0079061 | 85.443 | 158 | 23 | 0 | 1 | 158 | 118 | 275 | 8.95e-97 | 280 |
| Msa0393640 | MtrunA17_Chr3g0079131 | 62.420 | 157 | 55 | 1 | 6 | 158 | 119 | 275 | 2.46e-63 | 195 |
| Msa0393640 | MtrunA17_Chr3g0079091 | 81.667 | 60 | 11 | 0 | 1 | 60 | 118 | 177 | 5.65e-27 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTAGTGTATAGTGATT+TGG | 0.310655 | 3_2:+7712061 | Msa0393640:CDS |
| TGCTTTGAAATCTTTGAATC+TGG | 0.324623 | 3_2:+7712350 | Msa0393640:CDS |
| CTTTGAATCTGGTGAGGTAT+TGG | 0.325399 | 3_2:+7712361 | Msa0393640:CDS |
| AACAATTTAGCAAGAGAGTT+TGG | 0.341571 | 3_2:+7712272 | Msa0393640:CDS |
| GCAAGAGAGTTTGGTAGAAG+TGG | 0.402120 | 3_2:+7712281 | Msa0393640:CDS |
| TGTCATGAAGTGTTTCAATC+TGG | 0.412291 | 3_2:+7712209 | Msa0393640:CDS |
| AACATGCAATTTAATGTGGC+TGG | 0.457539 | 3_2:+7712014 | Msa0393640:CDS |
| ATATAATGTTGTAAATGAAA+AGG | 0.485017 | 3_2:+7712310 | Msa0393640:CDS |
| GTGTTATGCGTAAGAAAGAA+TGG | 0.501519 | 3_2:+7712108 | Msa0393640:CDS |
| TGAATCTGGTGAGGTATTGG+AGG | 0.506890 | 3_2:+7712364 | Msa0393640:CDS |
| GAAAGAATGGAAACAAATTG+AGG | 0.561440 | 3_2:+7712121 | Msa0393640:CDS |
| TGAAATCTTTGAATCTGGTG+AGG | 0.581368 | 3_2:+7712355 | Msa0393640:CDS |
| GGTATTGGAGGAACACATGA+AGG | 0.590503 | 3_2:+7712376 | Msa0393640:CDS |
| AGAGAACATGCAATTTAATG+TGG | 0.601137 | 3_2:+7712010 | Msa0393640:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTAAATTGTTAGTTTTATTA+TGG | - | chr3_2:7712262-7712281 | None:intergenic | 15.0% |
| !! | ATATAATGTTGTAAATGAAA+AGG | + | chr3_2:7712310-7712329 | Msa0393640:CDS | 15.0% |
| !! | AAAATTAGTGTATAGTGATT+TGG | + | chr3_2:7712061-7712080 | Msa0393640:CDS | 20.0% |
| ! | AAGAAAAAAGCAAGTGAAAA+TGG | + | chr3_2:7712161-7712180 | Msa0393640:CDS | 25.0% |
| AGAGAACATGCAATTTAATG+TGG | + | chr3_2:7712010-7712029 | Msa0393640:CDS | 30.0% | |
| GAAAGAATGGAAACAAATTG+AGG | + | chr3_2:7712121-7712140 | Msa0393640:CDS | 30.0% | |
| ! | CAATCTGGTAAAGATCTTTT+CGG | + | chr3_2:7712224-7712243 | Msa0393640:CDS | 30.0% |
| AACAATTTAGCAAGAGAGTT+TGG | + | chr3_2:7712272-7712291 | Msa0393640:CDS | 30.0% | |
| !! | TGCTTTGAAATCTTTGAATC+TGG | + | chr3_2:7712350-7712369 | Msa0393640:CDS | 30.0% |
| AACATGCAATTTAATGTGGC+TGG | + | chr3_2:7712014-7712033 | Msa0393640:CDS | 35.0% | |
| GTGTTATGCGTAAGAAAGAA+TGG | + | chr3_2:7712108-7712127 | Msa0393640:CDS | 35.0% | |
| ! | TGTCATGAAGTGTTTCAATC+TGG | + | chr3_2:7712209-7712228 | Msa0393640:CDS | 35.0% |
| TGAAATCTTTGAATCTGGTG+AGG | + | chr3_2:7712355-7712374 | Msa0393640:CDS | 35.0% | |
| CTTTGAATCTGGTGAGGTAT+TGG | + | chr3_2:7712361-7712380 | Msa0393640:CDS | 40.0% | |
| GCAAGAGAGTTTGGTAGAAG+TGG | + | chr3_2:7712281-7712300 | Msa0393640:CDS | 45.0% | |
| !! | TGAATCTGGTGAGGTATTGG+AGG | + | chr3_2:7712364-7712383 | Msa0393640:CDS | 45.0% |
| ! | GGTATTGGAGGAACACATGA+AGG | + | chr3_2:7712376-7712395 | Msa0393640:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 7711957 | 7712433 | 7711957 | ID=Msa0393640;Name=Msa0393640 |
| chr3_2 | mRNA | 7711957 | 7712433 | 7711957 | ID=Msa0393640-mRNA-1;Parent=Msa0393640;Name=Msa0393640-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|-1|0|1|-1|1|1|0|158 |
| chr3_2 | exon | 7711957 | 7712433 | 7711957 | ID=Msa0393640-mRNA-1:exon:2008;Parent=Msa0393640-mRNA-1 |
| chr3_2 | CDS | 7711957 | 7712433 | 7711957 | ID=Msa0393640-mRNA-1:cds;Parent=Msa0393640-mRNA-1 |
| Gene Sequence |
| Protein sequence |
