Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393700 | XP_013459208.1 | 90.182 | 275 | 22 | 2 | 1 | 270 | 1 | 275 | 4.08e-172 | 486 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393700 | sp|Q39092|ZAT1_ARATH | 26.296 | 270 | 148 | 9 | 5 | 263 | 4 | 233 | 1.84e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393700 | A0A072UTD1 | 90.182 | 275 | 22 | 2 | 1 | 270 | 1 | 275 | 1.95e-172 | 486 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0393700 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393700 | MtrunA17_Chr3g0079131 | 90.182 | 275 | 22 | 2 | 1 | 270 | 1 | 275 | 3.75e-176 | 486 |
| Msa0393700 | MtrunA17_Chr3g0079061 | 62.182 | 275 | 99 | 2 | 1 | 270 | 1 | 275 | 9.30e-114 | 328 |
| Msa0393700 | MtrunA17_Chr3g0079091 | 62.712 | 177 | 61 | 2 | 1 | 172 | 1 | 177 | 4.66e-63 | 196 |
| Msa0393700 | MtrunA17_Chr4g0018761 | 29.104 | 268 | 151 | 7 | 3 | 264 | 4 | 238 | 2.14e-25 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0393700 | AT1G02030.1 | 26.296 | 270 | 148 | 9 | 5 | 263 | 4 | 233 | 1.87e-12 | 66.2 |
| Msa0393700 | AT3G60580.1 | 27.240 | 279 | 142 | 11 | 7 | 261 | 5 | 246 | 2.84e-11 | 63.2 |
Find 25 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAAGATTATGCATAGGTTT+TGG | 0.234409 | 3_2:-7799543 | None:intergenic |
| CGGTCGTAGACAATTTAATT+TGG | 0.272723 | 3_2:+7799665 | Msa0393700:CDS |
| AACAATATATCTGAAGAAAT+TGG | 0.302487 | 3_2:+7799990 | Msa0393700:CDS |
| TGAGAATACACAAGTTAAAT+TGG | 0.302912 | 3_2:+7799764 | Msa0393700:CDS |
| ATACTATAGTGAGAAGAAAA+AGG | 0.312924 | 3_2:+7799866 | Msa0393700:CDS |
| TAAATTGGTGTATAGTGATT+TGG | 0.323391 | 3_2:+7799779 | Msa0393700:CDS |
| TCAGGTGCTTGGTTGTTGAT+TGG | 0.373001 | 3_2:-7799453 | None:intergenic |
| TCTGAAGAAATTGGTATAAG+TGG | 0.418644 | 3_2:+7799999 | Msa0393700:CDS |
| CAACTGAGTGTTCAGGTGCT+TGG | 0.424891 | 3_2:-7799464 | None:intergenic |
| CTATTATTTGTGTGAATGAA+TGG | 0.435873 | 3_2:+7799826 | Msa0393700:CDS |
| GTTGACTCAACTGAGTGTTC+AGG | 0.442669 | 3_2:-7799471 | None:intergenic |
| AATAGAGAGAGTTTGATGTT+TGG | 0.448411 | 3_2:-7799493 | None:intergenic |
| TATTCTTACTAAAGTTTACA+AGG | 0.462564 | 3_2:-7799593 | None:intergenic |
| TCACAAGTGCACATATTGCT+TGG | 0.480968 | 3_2:+7800052 | Msa0393700:CDS |
| CAATGCTTTCCCATTAGAAA+AGG | 0.484168 | 3_2:-7799379 | None:intergenic |
| TACAGAAGCTGCTGAAACAA+TGG | 0.521106 | 3_2:+7799803 | Msa0393700:CDS |
| TGTACTTGAGGAACACAAAA+CGG | 0.523616 | 3_2:+7800094 | Msa0393700:CDS |
| TGAATCTGATCATGTACTTG+AGG | 0.537940 | 3_2:+7800082 | Msa0393700:CDS |
| ATAATCTTCGATCTTTGAAG+AGG | 0.557615 | 3_2:+7799556 | Msa0393700:CDS |
| AGAAATTGGTATAAGTGGAA+AGG | 0.574832 | 3_2:+7800004 | Msa0393700:CDS |
| AAAGATCGAAGATTATGCAT+AGG | 0.576327 | 3_2:-7799549 | None:intergenic |
| TGTAGCTGAAGAAAATGAAG+AGG | 0.590626 | 3_2:+7799710 | Msa0393700:CDS |
| ATTCTTACTAAAGTTTACAA+GGG | 0.609953 | 3_2:-7799592 | None:intergenic |
| TCTAATGGGAAAGCATTGGG+AGG | 0.669450 | 3_2:+7799384 | Msa0393700:CDS |
| GAATGAATGGAAACAAGTTG+AGG | 0.698163 | 3_2:+7799839 | Msa0393700:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATATTGTTATTTTTCTTA+TGG | - | chr3_2:7799980-7799999 | None:intergenic | 10.0% |
| !!! | ATATATTGTTATTTTTCTTA+TGG | - | chr3_2:7799980-7799999 | None:intergenic | 10.0% |
| !! | ATTCTTACTAAAGTTTACAA+GGG | - | chr3_2:7799595-7799614 | None:intergenic | 20.0% |
| !! | TATTCTTACTAAAGTTTACA+AGG | - | chr3_2:7799596-7799615 | None:intergenic | 20.0% |
| !! | AACAATATATCTGAAGAAAT+TGG | + | chr3_2:7799990-7800009 | Msa0393700:CDS | 20.0% |
| !! | ATTCTTACTAAAGTTTACAA+GGG | - | chr3_2:7799595-7799614 | None:intergenic | 20.0% |
| !! | TATTCTTACTAAAGTTTACA+AGG | - | chr3_2:7799596-7799615 | None:intergenic | 20.0% |
| !! | AACAATATATCTGAAGAAAT+TGG | + | chr3_2:7799990-7800009 | Msa0393700:CDS | 20.0% |
| ! | TGAGAATACACAAGTTAAAT+TGG | + | chr3_2:7799764-7799783 | Msa0393700:CDS | 25.0% |
| ! | TAAATTGGTGTATAGTGATT+TGG | + | chr3_2:7799779-7799798 | Msa0393700:CDS | 25.0% |
| ! | CTATTATTTGTGTGAATGAA+TGG | + | chr3_2:7799826-7799845 | Msa0393700:CDS | 25.0% |
| ! | ATACTATAGTGAGAAGAAAA+AGG | + | chr3_2:7799866-7799885 | Msa0393700:CDS | 25.0% |
| ! | AAGAAAAAGGTTAGTGAAAA+TGG | + | chr3_2:7799879-7799898 | Msa0393700:CDS | 25.0% |
| ! | TGAGAATACACAAGTTAAAT+TGG | + | chr3_2:7799764-7799783 | Msa0393700:CDS | 25.0% |
| ! | TAAATTGGTGTATAGTGATT+TGG | + | chr3_2:7799779-7799798 | Msa0393700:CDS | 25.0% |
| ! | CTATTATTTGTGTGAATGAA+TGG | + | chr3_2:7799826-7799845 | Msa0393700:CDS | 25.0% |
| ! | ATACTATAGTGAGAAGAAAA+AGG | + | chr3_2:7799866-7799885 | Msa0393700:CDS | 25.0% |
| ! | AAGAAAAAGGTTAGTGAAAA+TGG | + | chr3_2:7799879-7799898 | Msa0393700:CDS | 25.0% |
| ! | TGTAACAAGTCCTTTTCTAA+TGG | + | chr3_2:7799369-7799388 | Msa0393700:CDS | 30.0% |
| ! | GTAACAAGTCCTTTTCTAAT+GGG | + | chr3_2:7799370-7799389 | Msa0393700:CDS | 30.0% |
| !!! | TTTTCTAATGGGAAAGCATT+GGG | + | chr3_2:7799381-7799400 | Msa0393700:CDS | 30.0% |
| !! | TGTTGATTGGAGATTTTAGA+GGG | - | chr3_2:7799443-7799462 | None:intergenic | 30.0% |
| !! | TTGTTGATTGGAGATTTTAG+AGG | - | chr3_2:7799444-7799463 | None:intergenic | 30.0% |
| AATAGAGAGAGTTTGATGTT+TGG | - | chr3_2:7799496-7799515 | None:intergenic | 30.0% | |
| !! | TTTTGGATTGGAAATTGATG+AGG | - | chr3_2:7799529-7799548 | None:intergenic | 30.0% |
| !!! | ATTATGCATAGGTTTTGGAT+TGG | - | chr3_2:7799541-7799560 | None:intergenic | 30.0% |
| AAAGATCGAAGATTATGCAT+AGG | - | chr3_2:7799552-7799571 | None:intergenic | 30.0% | |
| ! | ATAATCTTCGATCTTTGAAG+AGG | + | chr3_2:7799556-7799575 | Msa0393700:CDS | 30.0% |
| TCTGAAGAAATTGGTATAAG+TGG | + | chr3_2:7799999-7800018 | Msa0393700:CDS | 30.0% | |
| AGAAATTGGTATAAGTGGAA+AGG | + | chr3_2:7800004-7800023 | Msa0393700:CDS | 30.0% | |
| ! | TGGGTTAGAATCAGAAAAAT+TGG | - | chr3_2:7800129-7800148 | None:intergenic | 30.0% |
| ! | TGTAACAAGTCCTTTTCTAA+TGG | + | chr3_2:7799369-7799388 | Msa0393700:CDS | 30.0% |
| ! | GTAACAAGTCCTTTTCTAAT+GGG | + | chr3_2:7799370-7799389 | Msa0393700:CDS | 30.0% |
| !!! | TTTTCTAATGGGAAAGCATT+GGG | + | chr3_2:7799381-7799400 | Msa0393700:CDS | 30.0% |
| !! | TGTTGATTGGAGATTTTAGA+GGG | - | chr3_2:7799443-7799462 | None:intergenic | 30.0% |
| !! | TTGTTGATTGGAGATTTTAG+AGG | - | chr3_2:7799444-7799463 | None:intergenic | 30.0% |
| AATAGAGAGAGTTTGATGTT+TGG | - | chr3_2:7799496-7799515 | None:intergenic | 30.0% | |
| !! | TTTTGGATTGGAAATTGATG+AGG | - | chr3_2:7799529-7799548 | None:intergenic | 30.0% |
| !!! | ATTATGCATAGGTTTTGGAT+TGG | - | chr3_2:7799541-7799560 | None:intergenic | 30.0% |
| AAAGATCGAAGATTATGCAT+AGG | - | chr3_2:7799552-7799571 | None:intergenic | 30.0% | |
| ! | ATAATCTTCGATCTTTGAAG+AGG | + | chr3_2:7799556-7799575 | Msa0393700:CDS | 30.0% |
| TCTGAAGAAATTGGTATAAG+TGG | + | chr3_2:7799999-7800018 | Msa0393700:CDS | 30.0% | |
| AGAAATTGGTATAAGTGGAA+AGG | + | chr3_2:7800004-7800023 | Msa0393700:CDS | 30.0% | |
| ! | TGGGTTAGAATCAGAAAAAT+TGG | - | chr3_2:7800129-7800148 | None:intergenic | 30.0% |
| CAATGCTTTCCCATTAGAAA+AGG | - | chr3_2:7799382-7799401 | None:intergenic | 35.0% | |
| !! | CTTTTCTAATGGGAAAGCAT+TGG | + | chr3_2:7799380-7799399 | Msa0393700:CDS | 35.0% |
| ! | TAGCTAAGTGAGTTTTCATG+TGG | - | chr3_2:7799410-7799429 | None:intergenic | 35.0% |
| !! | ATTGGAGATTTTAGAGGGAT+TGG | - | chr3_2:7799438-7799457 | None:intergenic | 35.0% |
| ! | CGAAGATTATGCATAGGTTT+TGG | - | chr3_2:7799546-7799565 | None:intergenic | 35.0% |
| ! | TCTTTTCCAAAAAACCCAAC+TGG | + | chr3_2:7799627-7799646 | Msa0393700:CDS | 35.0% |
| ! | CTTTTTCCAGTTGGGTTTTT+TGG | - | chr3_2:7799636-7799655 | None:intergenic | 35.0% |
| ! | GTTTCGATCTTTTTCCAGTT+GGG | - | chr3_2:7799644-7799663 | None:intergenic | 35.0% |
| CGGTCGTAGACAATTTAATT+TGG | + | chr3_2:7799665-7799684 | Msa0393700:CDS | 35.0% | |
| TGTAGCTGAAGAAAATGAAG+AGG | + | chr3_2:7799710-7799729 | Msa0393700:CDS | 35.0% | |
| GAATGAATGGAAACAAGTTG+AGG | + | chr3_2:7799839-7799858 | Msa0393700:CDS | 35.0% | |
| TGAATCTGATCATGTACTTG+AGG | + | chr3_2:7800082-7800101 | Msa0393700:CDS | 35.0% | |
| TGTACTTGAGGAACACAAAA+CGG | + | chr3_2:7800094-7800113 | Msa0393700:CDS | 35.0% | |
| CAATGCTTTCCCATTAGAAA+AGG | - | chr3_2:7799382-7799401 | None:intergenic | 35.0% | |
| !! | CTTTTCTAATGGGAAAGCAT+TGG | + | chr3_2:7799380-7799399 | Msa0393700:CDS | 35.0% |
| ! | TAGCTAAGTGAGTTTTCATG+TGG | - | chr3_2:7799410-7799429 | None:intergenic | 35.0% |
| !! | ATTGGAGATTTTAGAGGGAT+TGG | - | chr3_2:7799438-7799457 | None:intergenic | 35.0% |
| ! | CGAAGATTATGCATAGGTTT+TGG | - | chr3_2:7799546-7799565 | None:intergenic | 35.0% |
| ! | TCTTTTCCAAAAAACCCAAC+TGG | + | chr3_2:7799627-7799646 | Msa0393700:CDS | 35.0% |
| ! | CTTTTTCCAGTTGGGTTTTT+TGG | - | chr3_2:7799636-7799655 | None:intergenic | 35.0% |
| ! | GTTTCGATCTTTTTCCAGTT+GGG | - | chr3_2:7799644-7799663 | None:intergenic | 35.0% |
| CGGTCGTAGACAATTTAATT+TGG | + | chr3_2:7799665-7799684 | Msa0393700:CDS | 35.0% | |
| TGTAGCTGAAGAAAATGAAG+AGG | + | chr3_2:7799710-7799729 | Msa0393700:CDS | 35.0% | |
| GAATGAATGGAAACAAGTTG+AGG | + | chr3_2:7799839-7799858 | Msa0393700:CDS | 35.0% | |
| TGAATCTGATCATGTACTTG+AGG | + | chr3_2:7800082-7800101 | Msa0393700:CDS | 35.0% | |
| TGTACTTGAGGAACACAAAA+CGG | + | chr3_2:7800094-7800113 | Msa0393700:CDS | 35.0% | |
| ! | CGTTTCGATCTTTTTCCAGT+TGG | - | chr3_2:7799645-7799664 | None:intergenic | 40.0% |
| ACTGGAAAAAGATCGAAACG+CGG | + | chr3_2:7799645-7799664 | Msa0393700:CDS | 40.0% | |
| TACAGAAGCTGCTGAAACAA+TGG | + | chr3_2:7799803-7799822 | Msa0393700:CDS | 40.0% | |
| TCACAAGTGCACATATTGCT+TGG | + | chr3_2:7800052-7800071 | Msa0393700:CDS | 40.0% | |
| ! | CGTTTCGATCTTTTTCCAGT+TGG | - | chr3_2:7799645-7799664 | None:intergenic | 40.0% |
| ACTGGAAAAAGATCGAAACG+CGG | + | chr3_2:7799645-7799664 | Msa0393700:CDS | 40.0% | |
| TACAGAAGCTGCTGAAACAA+TGG | + | chr3_2:7799803-7799822 | Msa0393700:CDS | 40.0% | |
| TCACAAGTGCACATATTGCT+TGG | + | chr3_2:7800052-7800071 | Msa0393700:CDS | 40.0% | |
| !! | TCTAATGGGAAAGCATTGGG+AGG | + | chr3_2:7799384-7799403 | Msa0393700:CDS | 45.0% |
| TCAGGTGCTTGGTTGTTGAT+TGG | - | chr3_2:7799456-7799475 | None:intergenic | 45.0% | |
| ! | GTTGACTCAACTGAGTGTTC+AGG | - | chr3_2:7799474-7799493 | None:intergenic | 45.0% |
| !! | TCTAATGGGAAAGCATTGGG+AGG | + | chr3_2:7799384-7799403 | Msa0393700:CDS | 45.0% |
| TCAGGTGCTTGGTTGTTGAT+TGG | - | chr3_2:7799456-7799475 | None:intergenic | 45.0% | |
| ! | GTTGACTCAACTGAGTGTTC+AGG | - | chr3_2:7799474-7799493 | None:intergenic | 45.0% |
| CAACTGAGTGTTCAGGTGCT+TGG | - | chr3_2:7799467-7799486 | None:intergenic | 50.0% | |
| CAACTGAGTGTTCAGGTGCT+TGG | - | chr3_2:7799467-7799486 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 7799339 | 7800151 | 7799339 | ID=Msa0393700;Name=Msa0393700 |
| chr3_2 | mRNA | 7799339 | 7800151 | 7799339 | ID=Msa0393700-mRNA-1;Parent=Msa0393700;Name=Msa0393700-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|270 |
| chr3_2 | exon | 7799339 | 7800151 | 7799339 | ID=Msa0393700-mRNA-1:exon:2030;Parent=Msa0393700-mRNA-1 |
| chr3_2 | CDS | 7799339 | 7800151 | 7799339 | ID=Msa0393700-mRNA-1:cds;Parent=Msa0393700-mRNA-1 |
| Gene Sequence |
| Protein sequence |
