AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0395760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0395760 MtrunA17_Chr3g0081631 98.901 91 1 0 1 91 1 91 1.80e-64 189
Msa0395760 MtrunA17_Chr7g0255841 70.000 80 23 1 12 91 14 92 2.71e-36 118
Msa0395760 MtrunA17_Chr3g0141601 58.242 91 33 2 4 90 1 90 6.43e-32 107
Msa0395760 MtrunA17_Chr1g0155181 58.621 87 29 3 4 84 1 86 1.16e-30 103
Msa0395760 MtrunA17_Chr6g0449111 62.667 75 27 1 1 75 1 74 1.37e-30 103
Msa0395760 MtrunA17_Chr3g0144631 64.198 81 22 2 1 75 1 80 5.22e-30 102
Msa0395760 MtrunA17_Chr8g0373111 56.522 69 28 1 7 75 2 68 1.07e-23 85.5
Msa0395760 MtrunA17_Chr8g0373121 56.522 69 28 1 7 75 2 68 1.07e-23 85.5
Msa0395760 MtrunA17_Chr8g0373131 57.353 68 27 1 7 74 2 67 1.16e-23 85.5
Msa0395760 MtrunA17_Chr5g0404301 59.375 64 24 1 8 71 2 63 3.86e-23 84.0
Msa0395760 MtrunA17_Chr8g0373101 59.375 64 24 1 8 71 3 64 5.18e-23 84.0
Msa0395760 MtrunA17_Chr5g0404101 48.649 74 37 1 1 74 10 82 1.58e-21 80.9
Msa0395760 MtrunA17_Chr5g0436501 49.315 73 36 1 1 73 18 89 1.72e-17 74.3
Msa0395760 MtrunA17_Chr3g0141641 38.462 91 46 2 9 90 2 91 1.89e-16 67.8
Msa0395760 MtrunA17_Chr8g0364741 44.737 76 41 1 1 76 1 75 3.68e-16 68.2
Msa0395760 MtrunA17_Chr5g0429521 46.429 84 33 2 9 81 28 110 6.62e-16 70.5
Msa0395760 MtrunA17_Chr7g0241761 44.706 85 37 2 10 85 35 118 1.63e-15 69.3
Msa0395760 MtrunA17_Chr3g0104071 50.704 71 34 1 3 73 36 105 3.28e-15 68.6
Msa0395760 MtrunA17_Chr1g0194071 52.632 57 27 0 10 66 11 67 5.68e-15 63.9
Msa0395760 MtrunA17_Chr7g0270711 54.237 59 27 0 10 68 11 69 6.85e-15 63.9
Msa0395760 MtrunA17_Chr8g0365011 48.529 68 34 1 6 73 4 70 1.63e-14 66.6
Msa0395760 MtrunA17_Chr1g0162081 42.667 75 42 1 1 75 3 76 1.48e-13 63.9
Msa0395760 MtrunA17_Chr4g0062741 44.737 76 37 3 4 75 1 75 8.10e-13 62.0
Msa0395760 MtrunA17_Chr1g0210471 40.909 66 38 1 10 75 24 88 3.05e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0395760 AT4G39250.1 60.227 88 28 2 10 91 14 100 2.09e-34 114
Msa0395760 AT2G21650.1 61.798 89 26 2 10 91 14 101 2.43e-34 114
Msa0395760 AT1G75250.2 53.684 95 35 3 3 90 5 97 1.14e-27 96.7
Msa0395760 AT1G75250.1 60.811 74 28 1 3 76 5 77 2.32e-26 94.4
Msa0395760 AT1G19510.1 51.724 87 33 3 13 91 15 100 2.41e-22 83.6
Msa0395760 AT2G18328.1 63.492 63 22 1 13 75 14 75 4.87e-22 82.0
Msa0395760 AT4G36570.1 68.889 45 13 1 10 54 12 55 8.54e-17 68.2
Msa0395760 AT5G58900.1 52.000 75 32 2 2 73 23 96 1.28e-16 72.8
Msa0395760 AT5G05790.2 49.351 77 36 2 7 81 28 103 1.30e-16 72.4
Msa0395760 AT5G05790.1 49.351 77 36 2 7 81 28 103 1.30e-16 72.4
Msa0395760 AT2G38090.1 50.649 77 31 2 10 80 29 104 1.79e-16 72.4
Msa0395760 AT3G11280.2 48.438 64 32 1 10 73 33 95 8.48e-14 64.7
Msa0395760 AT3G11280.1 48.438 64 32 1 10 73 33 95 8.48e-14 64.7
Msa0395760 AT5G23650.1 47.222 72 37 1 4 75 7 77 1.56e-12 61.6
Msa0395760 AT5G08520.1 48.438 64 32 1 10 73 12 74 1.02e-11 59.3
Msa0395760 AT5G04760.1 39.706 68 39 1 6 73 2 67 2.71e-11 57.4

Find 57 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCCAATGTTCTTGTAATT+TGG 0.053146 3_2:-12851147 None:intergenic
CCTCTAATTCTTCTTATAAA+AGG 0.137547 3_2:+12850714 Msa0395760:five_prime_UTR
CTCCAATGTTCTTGTAATTT+GGG 0.244052 3_2:-12851146 None:intergenic
GACACTATGAACACCTTCTA+AGG 0.262540 3_2:+12851088 Msa0395760:CDS
TGTTGACACCATTTGGTTAC+TGG 0.305364 3_2:-12851833 None:intergenic
CCACAACCTTCAAAATCTTA+AGG 0.319647 3_2:+12850842 Msa0395760:five_prime_UTR
CATTTGGTTACTGGAGCTTC+AGG 0.328788 3_2:-12851824 None:intergenic
GGTACACATGTGGTAATAAA+TGG 0.334394 3_2:-12852089 None:intergenic
TGAACCTTGGTTTCAAGAAA+TGG 0.338544 3_2:+12850918 Msa0395760:exon
CACAACCTTCAAAATCTTAA+GGG 0.363981 3_2:+12850843 Msa0395760:five_prime_UTR
TATTTGTTGTTGACACCATT+TGG 0.373580 3_2:-12851840 None:intergenic
TAACTATGATGTTACATAAC+AGG 0.375810 3_2:+12851922 Msa0395760:three_prime_UTR
TGTCTAAAGAATAAGGCTTA+AGG 0.395917 3_2:-12850774 None:intergenic
CTCTAATTCTTCTTATAAAA+GGG 0.397659 3_2:+12850715 Msa0395760:five_prime_UTR
TATGCCTAACATCCCTTAGA+AGG 0.404703 3_2:-12851101 None:intergenic
CAATGTCAGCATCTGGTTCA+TGG 0.413232 3_2:+12850944 Msa0395760:CDS
GATGTTAGGCATATTGAATC+AGG 0.424945 3_2:+12851111 Msa0395760:CDS
AAGGGTGTTCAAAATGTTGA+AGG 0.459490 3_2:-12850800 None:intergenic
TAGAGGGCGTGTGGAAGGTG+TGG 0.460077 3_2:-12850697 None:intergenic
AGAGGGCGTGTGGAAGGTGT+GGG 0.464590 3_2:-12850696 None:intergenic
AAGAATAAGGCTTAAGGAAG+AGG 0.466977 3_2:-12850768 None:intergenic
AACACCTTCTAAGGGATGTT+AGG 0.467379 3_2:+12851097 Msa0395760:CDS
TGAAGAGAGGAAGCAAGGTT+TGG 0.468195 3_2:-12850739 None:intergenic
TAGTGTCTCTTAACATCCTC+AGG 0.475467 3_2:-12851072 None:intergenic
CCAAATTACAAGAACATTGG+AGG 0.482774 3_2:+12851147 Msa0395760:CDS
GATATTCCAAATGACAAGAA+AGG 0.484250 3_2:-12852015 None:intergenic
AGAATTAGAGGGCGTGTGGA+AGG 0.485502 3_2:-12850702 None:intergenic
ATGGCATCAATGTCAGCATC+TGG 0.486683 3_2:+12850937 Msa0395760:CDS
ATATTCCAAATGACAAGAAA+GGG 0.487344 3_2:-12852014 None:intergenic
ACCAACAGCTTGAGCAACAT+TGG 0.499603 3_2:-12851037 None:intergenic
GATGTTGAAGAGAGGAAGCA+AGG 0.500934 3_2:-12850744 None:intergenic
TTCCCAAATTACAAGAACAT+TGG 0.502964 3_2:+12851144 Msa0395760:CDS
AGGACAATAAAGCATTTGAA+AGG 0.506069 3_2:+12850974 Msa0395760:CDS
AAGTTAACAAGATAAGCAAA+AGG 0.517696 3_2:-12850819 None:intergenic
TCCAATGTTGCTCAAGCTGT+TGG 0.520440 3_2:+12851036 Msa0395760:CDS
CTGAAGCTCCAGTAACCAAA+TGG 0.524122 3_2:+12851825 Msa0395760:exon
GGACAATAAAGCATTTGAAA+GGG 0.535274 3_2:+12850975 Msa0395760:CDS
GCAACATTGGACCAACGGTC+AGG 0.535547 3_2:-12851024 None:intergenic
GATGAAAAGAGATTATAGTA+AGG 0.535884 3_2:-12852110 None:intergenic
CTTGTCATTTGGAATATCAA+CGG 0.541338 3_2:+12852020 Msa0395760:three_prime_UTR
AATGTTGCTCAAGCTGTTGG+TGG 0.546053 3_2:+12851039 Msa0395760:CDS
GTTGCTCAAGCTGTTGGTGG+TGG 0.554528 3_2:+12851042 Msa0395760:CDS
TTTCACTAATGCTACATCAC+TGG 0.559072 3_2:-12851950 None:intergenic
AAGAAGAATTAGAGGGCGTG+TGG 0.560374 3_2:-12850706 None:intergenic
GAAGAGGTGATGTTGAAGAG+AGG 0.562902 3_2:-12850752 None:intergenic
GATGCCATTTCTTGAAACCA+AGG 0.568023 3_2:-12850922 None:intergenic
AGTTAACAAGATAAGCAAAA+GGG 0.571694 3_2:-12850818 None:intergenic
ATCTGGTTCATGGAGTGCTA+AGG 0.583163 3_2:+12850954 Msa0395760:CDS
GAGGGCGTGTGGAAGGTGTG+GGG 0.588905 3_2:-12850695 None:intergenic
ATAAAGACACACCTGACCGT+TGG 0.596639 3_2:+12851013 Msa0395760:CDS
ACACTATGAACACCTTCTAA+GGG 0.610400 3_2:+12851089 Msa0395760:CDS
CTTGAGCAACATTGGACCAA+CGG 0.640722 3_2:-12851029 None:intergenic
TATTCCAAATGACAAGAAAG+GGG 0.657237 3_2:-12852013 None:intergenic
GAACATTGGAGGCTATGATG+AGG 0.660409 3_2:+12851158 Msa0395760:CDS
ATTCCAAATGACAAGAAAGG+GGG 0.672297 3_2:-12852012 None:intergenic
TGGTGGTGGAAAGACTCCTG+AGG 0.678384 3_2:+12851056 Msa0395760:CDS
ATTATAGTAAGGTACACATG+TGG 0.707926 3_2:-12852099 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTTATAAACATATATT+AGG + chr3_2:12851209-12851228 Msa0395760:intron 10.0%
!!! TATTTTTATGTATTAATTGT+TGG + chr3_2:12851600-12851619 Msa0395760:intron 10.0%
!!! ATTTTTATGTATTAATTGTT+GGG + chr3_2:12851601-12851620 Msa0395760:intron 10.0%
!! ATTAATAAAAAATGTAAGTT+AGG - chr3_2:12851713-12851732 None:intergenic 10.0%
!!! ATAAAGCTATAATTTTATTA+AGG - chr3_2:12852064-12852083 None:intergenic 10.0%
!! ATGTACTAAAAAAATATATT+AGG + chr3_2:12852191-12852210 Msa0395760:three_prime_UTR 10.0%
!!! TTTTCTTATAAACATATATT+AGG + chr3_2:12851209-12851228 Msa0395760:intron 10.0%
!!! TATTTTTATGTATTAATTGT+TGG + chr3_2:12851600-12851619 Msa0395760:intron 10.0%
!!! ATTTTTATGTATTAATTGTT+GGG + chr3_2:12851601-12851620 Msa0395760:intron 10.0%
!! ATTAATAAAAAATGTAAGTT+AGG - chr3_2:12851713-12851732 None:intergenic 10.0%
!!! ATAAAGCTATAATTTTATTA+AGG - chr3_2:12852064-12852083 None:intergenic 10.0%
!! ATGTACTAAAAAAATATATT+AGG + chr3_2:12852191-12852210 Msa0395760:three_prime_UTR 10.0%
!! ACACATGTTAAAAAAAAAAT+TGG - chr3_2:12851688-12851707 None:intergenic 15.0%
!!! GTTTTTATTTTTAGAATCAT+TGG + chr3_2:12851893-12851912 Msa0395760:three_prime_UTR 15.0%
!! ACACATGTTAAAAAAAAAAT+TGG - chr3_2:12851688-12851707 None:intergenic 15.0%
!!! GTTTTTATTTTTAGAATCAT+TGG + chr3_2:12851893-12851912 Msa0395760:three_prime_UTR 15.0%
!!! CTTTTATAAGAAGAATTAGA+GGG - chr3_2:12850716-12850735 None:intergenic 20.0%
!! CTCTAATTCTTCTTATAAAA+GGG + chr3_2:12850715-12850734 Msa0395760:five_prime_UTR 20.0%
!!! ACAAAAACAAGAAATTTGTT+TGG + chr3_2:12850886-12850905 Msa0395760:five_prime_UTR 20.0%
!! TCTTATAAACATATATTAGG+AGG + chr3_2:12851212-12851231 Msa0395760:intron 20.0%
!!! AGAATTTGATAGCATAAATT+CGG - chr3_2:12851543-12851562 None:intergenic 20.0%
!!! GCTATAATTTTATTAAGGTT+GGG - chr3_2:12852059-12852078 None:intergenic 20.0%
!!! AGCTATAATTTTATTAAGGT+TGG - chr3_2:12852060-12852079 None:intergenic 20.0%
!!! CTTTTATAAGAAGAATTAGA+GGG - chr3_2:12850716-12850735 None:intergenic 20.0%
!! CTCTAATTCTTCTTATAAAA+GGG + chr3_2:12850715-12850734 Msa0395760:five_prime_UTR 20.0%
!!! ACAAAAACAAGAAATTTGTT+TGG + chr3_2:12850886-12850905 Msa0395760:five_prime_UTR 20.0%
!! TCTTATAAACATATATTAGG+AGG + chr3_2:12851212-12851231 Msa0395760:intron 20.0%
!!! AGAATTTGATAGCATAAATT+CGG - chr3_2:12851543-12851562 None:intergenic 20.0%
!!! GCTATAATTTTATTAAGGTT+GGG - chr3_2:12852059-12852078 None:intergenic 20.0%
!!! AGCTATAATTTTATTAAGGT+TGG - chr3_2:12852060-12852079 None:intergenic 20.0%
!! CCTTTTATAAGAAGAATTAG+AGG - chr3_2:12850717-12850736 None:intergenic 25.0%
! CCTCTAATTCTTCTTATAAA+AGG + chr3_2:12850714-12850733 Msa0395760:five_prime_UTR 25.0%
!!! AAGGTTTTGTCTAAAGAATA+AGG - chr3_2:12850784-12850803 None:intergenic 25.0%
! AGTTAACAAGATAAGCAAAA+GGG - chr3_2:12850821-12850840 None:intergenic 25.0%
! AAGTTAACAAGATAAGCAAA+AGG - chr3_2:12850822-12850841 None:intergenic 25.0%
! ATAGTGAGAATGAAAAATGA+TGG + chr3_2:12851504-12851523 Msa0395760:intron 25.0%
!! GAATTTGATAGCATAAATTC+GGG - chr3_2:12851542-12851561 None:intergenic 25.0%
!!! ACATGTCAAATTTTGACTAA+AGG - chr3_2:12851655-12851674 None:intergenic 25.0%
!!! ATTTTTAGAATCATTGGACT+AGG + chr3_2:12851899-12851918 Msa0395760:three_prime_UTR 25.0%
! TAACTATGATGTTACATAAC+AGG + chr3_2:12851922-12851941 Msa0395760:three_prime_UTR 25.0%
! ATATTCCAAATGACAAGAAA+GGG - chr3_2:12852017-12852036 None:intergenic 25.0%
! GATGAAAAGAGATTATAGTA+AGG - chr3_2:12852113-12852132 None:intergenic 25.0%
!! CCTTTTATAAGAAGAATTAG+AGG - chr3_2:12850717-12850736 None:intergenic 25.0%
! CCTCTAATTCTTCTTATAAA+AGG + chr3_2:12850714-12850733 Msa0395760:five_prime_UTR 25.0%
!!! AAGGTTTTGTCTAAAGAATA+AGG - chr3_2:12850784-12850803 None:intergenic 25.0%
! AGTTAACAAGATAAGCAAAA+GGG - chr3_2:12850821-12850840 None:intergenic 25.0%
! AAGTTAACAAGATAAGCAAA+AGG - chr3_2:12850822-12850841 None:intergenic 25.0%
! ATAGTGAGAATGAAAAATGA+TGG + chr3_2:12851504-12851523 Msa0395760:intron 25.0%
!! GAATTTGATAGCATAAATTC+GGG - chr3_2:12851542-12851561 None:intergenic 25.0%
!!! ACATGTCAAATTTTGACTAA+AGG - chr3_2:12851655-12851674 None:intergenic 25.0%
!!! ATTTTTAGAATCATTGGACT+AGG + chr3_2:12851899-12851918 Msa0395760:three_prime_UTR 25.0%
! TAACTATGATGTTACATAAC+AGG + chr3_2:12851922-12851941 Msa0395760:three_prime_UTR 25.0%
! ATATTCCAAATGACAAGAAA+GGG - chr3_2:12852017-12852036 None:intergenic 25.0%
! GATGAAAAGAGATTATAGTA+AGG - chr3_2:12852113-12852132 None:intergenic 25.0%
TGTCTAAAGAATAAGGCTTA+AGG - chr3_2:12850777-12850796 None:intergenic 30.0%
CACAACCTTCAAAATCTTAA+GGG + chr3_2:12850843-12850862 Msa0395760:five_prime_UTR 30.0%
! AGGACAATAAAGCATTTGAA+AGG + chr3_2:12850974-12850993 Msa0395760:CDS 30.0%
! GGACAATAAAGCATTTGAAA+GGG + chr3_2:12850975-12850994 Msa0395760:CDS 30.0%
TTCCCAAATTACAAGAACAT+TGG + chr3_2:12851144-12851163 Msa0395760:CDS 30.0%
CTCCAATGTTCTTGTAATTT+GGG - chr3_2:12851149-12851168 None:intergenic 30.0%
ACGCTGAATAAGTATTATGA+AGG - chr3_2:12851258-12851277 None:intergenic 30.0%
ATCTCTCAAGCATATAAAAG+TGG + chr3_2:12851572-12851591 Msa0395760:intron 30.0%
! CTTCTTGAGTTTAACTTTCT+TGG + chr3_2:12851769-12851788 Msa0395760:intron 30.0%
!! TATTTGTTGTTGACACCATT+TGG - chr3_2:12851843-12851862 None:intergenic 30.0%
TATTCCAAATGACAAGAAAG+GGG - chr3_2:12852016-12852035 None:intergenic 30.0%
GATATTCCAAATGACAAGAA+AGG - chr3_2:12852018-12852037 None:intergenic 30.0%
! CTTGTCATTTGGAATATCAA+CGG + chr3_2:12852020-12852039 Msa0395760:three_prime_UTR 30.0%
ATTATAGTAAGGTACACATG+TGG - chr3_2:12852102-12852121 None:intergenic 30.0%
TGTCTAAAGAATAAGGCTTA+AGG - chr3_2:12850777-12850796 None:intergenic 30.0%
CACAACCTTCAAAATCTTAA+GGG + chr3_2:12850843-12850862 Msa0395760:five_prime_UTR 30.0%
! AGGACAATAAAGCATTTGAA+AGG + chr3_2:12850974-12850993 Msa0395760:CDS 30.0%
! GGACAATAAAGCATTTGAAA+GGG + chr3_2:12850975-12850994 Msa0395760:CDS 30.0%
TTCCCAAATTACAAGAACAT+TGG + chr3_2:12851144-12851163 Msa0395760:CDS 30.0%
CTCCAATGTTCTTGTAATTT+GGG - chr3_2:12851149-12851168 None:intergenic 30.0%
ACGCTGAATAAGTATTATGA+AGG - chr3_2:12851258-12851277 None:intergenic 30.0%
ATCTCTCAAGCATATAAAAG+TGG + chr3_2:12851572-12851591 Msa0395760:intron 30.0%
! CTTCTTGAGTTTAACTTTCT+TGG + chr3_2:12851769-12851788 Msa0395760:intron 30.0%
!! TATTTGTTGTTGACACCATT+TGG - chr3_2:12851843-12851862 None:intergenic 30.0%
TATTCCAAATGACAAGAAAG+GGG - chr3_2:12852016-12852035 None:intergenic 30.0%
GATATTCCAAATGACAAGAA+AGG - chr3_2:12852018-12852037 None:intergenic 30.0%
! CTTGTCATTTGGAATATCAA+CGG + chr3_2:12852020-12852039 Msa0395760:three_prime_UTR 30.0%
ATTATAGTAAGGTACACATG+TGG - chr3_2:12852102-12852121 None:intergenic 30.0%
AAGAATAAGGCTTAAGGAAG+AGG - chr3_2:12850771-12850790 None:intergenic 35.0%
!! AAGGGTGTTCAAAATGTTGA+AGG - chr3_2:12850803-12850822 None:intergenic 35.0%
!! CCTTAAGATTTTGAAGGTTG+TGG - chr3_2:12850845-12850864 None:intergenic 35.0%
CCACAACCTTCAAAATCTTA+AGG + chr3_2:12850842-12850861 Msa0395760:five_prime_UTR 35.0%
! ATGTGCCCTTAAGATTTTGA+AGG - chr3_2:12850851-12850870 None:intergenic 35.0%
!!! TTGGACTTAGTTTTGAACCT+TGG + chr3_2:12850905-12850924 Msa0395760:five_prime_UTR 35.0%
TGAACCTTGGTTTCAAGAAA+TGG + chr3_2:12850918-12850937 Msa0395760:exon 35.0%
ACACTATGAACACCTTCTAA+GGG + chr3_2:12851089-12851108 Msa0395760:CDS 35.0%
GATGTTAGGCATATTGAATC+AGG + chr3_2:12851111-12851130 Msa0395760:CDS 35.0%
CCTCCAATGTTCTTGTAATT+TGG - chr3_2:12851150-12851169 None:intergenic 35.0%
CCAAATTACAAGAACATTGG+AGG + chr3_2:12851147-12851166 Msa0395760:CDS 35.0%
!! AACTCATATCGCTGATTTAC+TGG + chr3_2:12851281-12851300 Msa0395760:intron 35.0%
AGCATAAATTCGGGTATGTT+CGG - chr3_2:12851533-12851552 None:intergenic 35.0%
ATTAATTGTTGGGTCAAGTG+TGG + chr3_2:12851611-12851630 Msa0395760:intron 35.0%
TTAATTGTTGGGTCAAGTGT+GGG + chr3_2:12851612-12851631 Msa0395760:intron 35.0%
TTTCACTAATGCTACATCAC+TGG - chr3_2:12851953-12851972 None:intergenic 35.0%
ATTCCAAATGACAAGAAAGG+GGG - chr3_2:12852015-12852034 None:intergenic 35.0%
GGTACACATGTGGTAATAAA+TGG - chr3_2:12852092-12852111 None:intergenic 35.0%
AAGAATAAGGCTTAAGGAAG+AGG - chr3_2:12850771-12850790 None:intergenic 35.0%
!! AAGGGTGTTCAAAATGTTGA+AGG - chr3_2:12850803-12850822 None:intergenic 35.0%
!! CCTTAAGATTTTGAAGGTTG+TGG - chr3_2:12850845-12850864 None:intergenic 35.0%
CCACAACCTTCAAAATCTTA+AGG + chr3_2:12850842-12850861 Msa0395760:five_prime_UTR 35.0%
! ATGTGCCCTTAAGATTTTGA+AGG - chr3_2:12850851-12850870 None:intergenic 35.0%
!!! TTGGACTTAGTTTTGAACCT+TGG + chr3_2:12850905-12850924 Msa0395760:five_prime_UTR 35.0%
TGAACCTTGGTTTCAAGAAA+TGG + chr3_2:12850918-12850937 Msa0395760:exon 35.0%
ACACTATGAACACCTTCTAA+GGG + chr3_2:12851089-12851108 Msa0395760:CDS 35.0%
GATGTTAGGCATATTGAATC+AGG + chr3_2:12851111-12851130 Msa0395760:CDS 35.0%
CCTCCAATGTTCTTGTAATT+TGG - chr3_2:12851150-12851169 None:intergenic 35.0%
CCAAATTACAAGAACATTGG+AGG + chr3_2:12851147-12851166 Msa0395760:CDS 35.0%
!! AACTCATATCGCTGATTTAC+TGG + chr3_2:12851281-12851300 Msa0395760:intron 35.0%
AGCATAAATTCGGGTATGTT+CGG - chr3_2:12851533-12851552 None:intergenic 35.0%
ATTAATTGTTGGGTCAAGTG+TGG + chr3_2:12851611-12851630 Msa0395760:intron 35.0%
TTAATTGTTGGGTCAAGTGT+GGG + chr3_2:12851612-12851631 Msa0395760:intron 35.0%
TTTCACTAATGCTACATCAC+TGG - chr3_2:12851953-12851972 None:intergenic 35.0%
ATTCCAAATGACAAGAAAGG+GGG - chr3_2:12852015-12852034 None:intergenic 35.0%
GGTACACATGTGGTAATAAA+TGG - chr3_2:12852092-12852111 None:intergenic 35.0%
! GATGCCATTTCTTGAAACCA+AGG - chr3_2:12850925-12850944 None:intergenic 40.0%
TAGTGTCTCTTAACATCCTC+AGG - chr3_2:12851075-12851094 None:intergenic 40.0%
GACACTATGAACACCTTCTA+AGG + chr3_2:12851088-12851107 Msa0395760:CDS 40.0%
! AACACCTTCTAAGGGATGTT+AGG + chr3_2:12851097-12851116 Msa0395760:CDS 40.0%
TATGCCTAACATCCCTTAGA+AGG - chr3_2:12851104-12851123 None:intergenic 40.0%
GAGGCTATGATGAGGAAAAA+AGG + chr3_2:12851166-12851185 Msa0395760:CDS 40.0%
TAATTGTTGGGTCAAGTGTG+GGG + chr3_2:12851613-12851632 Msa0395760:intron 40.0%
AACCTGCAAGTGAAAACAAG+AGG - chr3_2:12851798-12851817 None:intergenic 40.0%
TGTTGACACCATTTGGTTAC+TGG - chr3_2:12851836-12851855 None:intergenic 40.0%
! GATGCCATTTCTTGAAACCA+AGG - chr3_2:12850925-12850944 None:intergenic 40.0%
TAGTGTCTCTTAACATCCTC+AGG - chr3_2:12851075-12851094 None:intergenic 40.0%
GACACTATGAACACCTTCTA+AGG + chr3_2:12851088-12851107 Msa0395760:CDS 40.0%
! AACACCTTCTAAGGGATGTT+AGG + chr3_2:12851097-12851116 Msa0395760:CDS 40.0%
TATGCCTAACATCCCTTAGA+AGG - chr3_2:12851104-12851123 None:intergenic 40.0%
GAGGCTATGATGAGGAAAAA+AGG + chr3_2:12851166-12851185 Msa0395760:CDS 40.0%
TAATTGTTGGGTCAAGTGTG+GGG + chr3_2:12851613-12851632 Msa0395760:intron 40.0%
AACCTGCAAGTGAAAACAAG+AGG - chr3_2:12851798-12851817 None:intergenic 40.0%
TGTTGACACCATTTGGTTAC+TGG - chr3_2:12851836-12851855 None:intergenic 40.0%
! AAGAAGAATTAGAGGGCGTG+TGG - chr3_2:12850709-12850728 None:intergenic 45.0%
TGAAGAGAGGAAGCAAGGTT+TGG - chr3_2:12850742-12850761 None:intergenic 45.0%
GATGTTGAAGAGAGGAAGCA+AGG - chr3_2:12850747-12850766 None:intergenic 45.0%
GAAGAGGTGATGTTGAAGAG+AGG - chr3_2:12850755-12850774 None:intergenic 45.0%
ATGGCATCAATGTCAGCATC+TGG + chr3_2:12850937-12850956 Msa0395760:CDS 45.0%
! CAATGTCAGCATCTGGTTCA+TGG + chr3_2:12850944-12850963 Msa0395760:CDS 45.0%
! ATCTGGTTCATGGAGTGCTA+AGG + chr3_2:12850954-12850973 Msa0395760:CDS 45.0%
ATAAAGACACACCTGACCGT+TGG + chr3_2:12851013-12851032 Msa0395760:CDS 45.0%
CTTGAGCAACATTGGACCAA+CGG - chr3_2:12851032-12851051 None:intergenic 45.0%
TCCAATGTTGCTCAAGCTGT+TGG + chr3_2:12851036-12851055 Msa0395760:CDS 45.0%
ACCAACAGCTTGAGCAACAT+TGG - chr3_2:12851040-12851059 None:intergenic 45.0%
AATGTTGCTCAAGCTGTTGG+TGG + chr3_2:12851039-12851058 Msa0395760:CDS 45.0%
GAACATTGGAGGCTATGATG+AGG + chr3_2:12851158-12851177 Msa0395760:CDS 45.0%
!!! TGCCTCTTGTTTTCACTTGC+AGG + chr3_2:12851793-12851812 Msa0395760:intron 45.0%
CATTTGGTTACTGGAGCTTC+AGG - chr3_2:12851827-12851846 None:intergenic 45.0%
CTGAAGCTCCAGTAACCAAA+TGG + chr3_2:12851825-12851844 Msa0395760:exon 45.0%
AAAGGGGGACATGGAATCAT+GGG - chr3_2:12852000-12852019 None:intergenic 45.0%
AATGACAAGAAAGGGGGACA+TGG - chr3_2:12852009-12852028 None:intergenic 45.0%
TGTCCCCCTTTCTTGTCATT+TGG + chr3_2:12852009-12852028 Msa0395760:three_prime_UTR 45.0%
! AAGAAGAATTAGAGGGCGTG+TGG - chr3_2:12850709-12850728 None:intergenic 45.0%
TGAAGAGAGGAAGCAAGGTT+TGG - chr3_2:12850742-12850761 None:intergenic 45.0%
GATGTTGAAGAGAGGAAGCA+AGG - chr3_2:12850747-12850766 None:intergenic 45.0%
GAAGAGGTGATGTTGAAGAG+AGG - chr3_2:12850755-12850774 None:intergenic 45.0%
ATGGCATCAATGTCAGCATC+TGG + chr3_2:12850937-12850956 Msa0395760:CDS 45.0%
! CAATGTCAGCATCTGGTTCA+TGG + chr3_2:12850944-12850963 Msa0395760:CDS 45.0%
! ATCTGGTTCATGGAGTGCTA+AGG + chr3_2:12850954-12850973 Msa0395760:CDS 45.0%
ATAAAGACACACCTGACCGT+TGG + chr3_2:12851013-12851032 Msa0395760:CDS 45.0%
CTTGAGCAACATTGGACCAA+CGG - chr3_2:12851032-12851051 None:intergenic 45.0%
TCCAATGTTGCTCAAGCTGT+TGG + chr3_2:12851036-12851055 Msa0395760:CDS 45.0%
ACCAACAGCTTGAGCAACAT+TGG - chr3_2:12851040-12851059 None:intergenic 45.0%
AATGTTGCTCAAGCTGTTGG+TGG + chr3_2:12851039-12851058 Msa0395760:CDS 45.0%
GAACATTGGAGGCTATGATG+AGG + chr3_2:12851158-12851177 Msa0395760:CDS 45.0%
!!! TGCCTCTTGTTTTCACTTGC+AGG + chr3_2:12851793-12851812 Msa0395760:intron 45.0%
CATTTGGTTACTGGAGCTTC+AGG - chr3_2:12851827-12851846 None:intergenic 45.0%
CTGAAGCTCCAGTAACCAAA+TGG + chr3_2:12851825-12851844 Msa0395760:exon 45.0%
AAAGGGGGACATGGAATCAT+GGG - chr3_2:12852000-12852019 None:intergenic 45.0%
AATGACAAGAAAGGGGGACA+TGG - chr3_2:12852009-12852028 None:intergenic 45.0%
TGTCCCCCTTTCTTGTCATT+TGG + chr3_2:12852009-12852028 Msa0395760:three_prime_UTR 45.0%
! AGAATTAGAGGGCGTGTGGA+AGG - chr3_2:12850705-12850724 None:intergenic 50.0%
GAAAGGGGGACATGGAATCA+TGG - chr3_2:12852001-12852020 None:intergenic 50.0%
! AGAATTAGAGGGCGTGTGGA+AGG - chr3_2:12850705-12850724 None:intergenic 50.0%
GAAAGGGGGACATGGAATCA+TGG - chr3_2:12852001-12852020 None:intergenic 50.0%
GCAACATTGGACCAACGGTC+AGG - chr3_2:12851027-12851046 None:intergenic 55.0%
! GTTGCTCAAGCTGTTGGTGG+TGG + chr3_2:12851042-12851061 Msa0395760:CDS 55.0%
!! TGGTGGTGGAAAGACTCCTG+AGG + chr3_2:12851056-12851075 Msa0395760:CDS 55.0%
GCAACATTGGACCAACGGTC+AGG - chr3_2:12851027-12851046 None:intergenic 55.0%
! GTTGCTCAAGCTGTTGGTGG+TGG + chr3_2:12851042-12851061 Msa0395760:CDS 55.0%
!! TGGTGGTGGAAAGACTCCTG+AGG + chr3_2:12851056-12851075 Msa0395760:CDS 55.0%
! AGAGGGCGTGTGGAAGGTGT+GGG - chr3_2:12850699-12850718 None:intergenic 60.0%
! TAGAGGGCGTGTGGAAGGTG+TGG - chr3_2:12850700-12850719 None:intergenic 60.0%
! AGAGGGCGTGTGGAAGGTGT+GGG - chr3_2:12850699-12850718 None:intergenic 60.0%
! TAGAGGGCGTGTGGAAGGTG+TGG - chr3_2:12850700-12850719 None:intergenic 60.0%
! GAGGGCGTGTGGAAGGTGTG+GGG - chr3_2:12850698-12850717 None:intergenic 65.0%
! GAGGGCGTGTGGAAGGTGTG+GGG - chr3_2:12850698-12850717 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 12850686 12852222 12850686 ID=Msa0395760;Name=Msa0395760
chr3_2 mRNA 12850686 12852222 12850686 ID=Msa0395760-mRNA-1;Parent=Msa0395760;Name=Msa0395760-mRNA-1;_AED=0.01;_eAED=0.01;_QI=251|1|1|1|1|1|2|383|91
chr3_2 exon 12850686 12851187 12850686 ID=Msa0395760-mRNA-1:exon:2988;Parent=Msa0395760-mRNA-1
chr3_2 exon 12851815 12852222 12851815 ID=Msa0395760-mRNA-1:exon:2989;Parent=Msa0395760-mRNA-1
chr3_2 five_prime_UTR 12850686 12850936 12850686 ID=Msa0395760-mRNA-1:five_prime_utr;Parent=Msa0395760-mRNA-1
chr3_2 CDS 12850937 12851187 12850937 ID=Msa0395760-mRNA-1:cds;Parent=Msa0395760-mRNA-1
chr3_2 CDS 12851815 12851839 12851815 ID=Msa0395760-mRNA-1:cds;Parent=Msa0395760-mRNA-1
chr3_2 three_prime_UTR 12851840 12852222 12851840 ID=Msa0395760-mRNA-1:three_prime_utr;Parent=Msa0395760-mRNA-1
Gene Sequence

>Msa0395760

ATGGCATCAATGTCAGCATCTGGTTCATGGAGTGCTAAGGACAATAAAGCATTTGAAAGGGCACTAGCTGTTTTTGATAAAGACACACCTGACCGTTGGTCCAATGTTGCTCAAGCTGTTGGTGGTGGAAAGACTCCTGAGGATGTTAAGAGACACTATGAACACCTTCTAAGGGATGTTAGGCATATTGAATCAGGTCATGTTGCATTCCCAAATTACAAGAACATTGGAGGCTATGATGAGGAAAAAAGGTTGAGAAACCTGAAGCTCCAGTAA
Protein sequence

>Msa0395760

MASMSASGSWSAKDNKAFERALAVFDKDTPDRWSNVAQAVGGGKTPEDVKRHYEHLLRDVRHIESGHVAFPNYKNIGGYDEEKRLRNLKLQ*