AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0396690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0396690 MtrunA17_Chr1g0177151 60.700 257 97 1 1 257 1 253 2.92e-112 325
Msa0396690 MtrunA17_Chr7g0224601 63.273 275 97 2 12 282 2 276 6.51e-107 313
Msa0396690 MtrunA17_Chr8g0392781 53.312 317 113 2 1 282 1 317 3.12e-104 308
Msa0396690 MtrunA17_Chr3g0099561 61.633 245 94 0 38 282 26 270 6.84e-100 295
Msa0396690 MtrunA17_Chr5g0444621 61.927 218 83 0 70 287 52 269 1.41e-88 265
Msa0396690 MtrunA17_Chr6g0477171 62.312 199 75 0 59 257 45 243 3.33e-81 246
Msa0396690 MtrunA17_Chr3g0083811 94.845 97 5 0 186 282 1 97 1.34e-59 186
Msa0396690 MtrunA17_Chr3g0083821 72.973 111 30 0 54 164 10 120 1.18e-50 164
Msa0396690 MtrunA17_Chr5g0444631 66.038 53 18 0 112 164 4 56 1.76e-18 77.8
Msa0396690 MtrunA17_Chr6g0477181 61.017 59 23 0 1 59 1 59 1.20e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0396690 AT3G20800.1 57.303 267 109 1 21 282 17 283 2.88e-100 297
Msa0396690 AT5G12980.1 55.510 245 109 0 31 275 28 272 6.84e-94 281
Msa0396690 AT2G32550.2 35.271 258 150 6 53 297 67 320 4.55e-34 127
Msa0396690 AT2G32550.3 35.628 247 142 6 64 297 39 281 1.02e-33 125
Msa0396690 AT2G32550.1 35.371 229 131 6 82 297 2 226 6.91e-29 110

Find 48 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAACTTGGACCTTTATTA+TGG 0.221177 3_2:-15438962 Msa0396690:CDS
ATATTAAATTATATCATATT+AGG 0.243236 3_2:+15436136 None:intergenic
CTCATTTATCTCAAAATTGC+AGG 0.284194 3_2:-15436376 Msa0396690:intron
AGAAATGAGATCCTTGTATC+AGG 0.301701 3_2:+15437954 None:intergenic
ATAGAAACCTATGACTTCTT+TGG 0.317203 3_2:+15436739 None:intergenic
AGGAATGGATATAAATATAA+AGG 0.360461 3_2:+15437562 None:intergenic
GTTGCTAGTCTTGGAGTCAT+TGG 0.372727 3_2:-15437499 Msa0396690:CDS
TATATTAGACGATGATGGTT+TGG 0.376298 3_2:-15436519 Msa0396690:CDS
TTGGCATCTTTGAGCCTGTT+TGG 0.379455 3_2:+15436232 None:intergenic
CCAACAGTATTCCATAATAA+AGG 0.383147 3_2:+15438951 None:intergenic
TATGATCAGTGGTGCTAGAT+TGG 0.401740 3_2:+15440679 None:intergenic
GTTAACACCAAAGAAGTCAT+AGG 0.420089 3_2:-15436746 Msa0396690:CDS
CAATGTTCTAGCATTACTTC+AGG 0.422671 3_2:-15438185 Msa0396690:intron
TCAGAGCACCAAGTTATTTG+AGG 0.428345 3_2:-15438982 Msa0396690:intron
TAACAACTTATCTTCCAAAC+AGG 0.438040 3_2:-15436246 Msa0396690:CDS
TAACACCCTTTGAAAATTTG+AGG 0.438320 3_2:-15437522 Msa0396690:CDS
TTCTTTGTTGAGAGCGATTG+AGG 0.439462 3_2:+15440738 None:intergenic
GATTGTGAGGGAGAGAGATT+CGG 0.440290 3_2:+15438220 None:intergenic
AATTTGAGGGTTGCTAGTCT+TGG 0.449868 3_2:-15437508 Msa0396690:CDS
ATGATCAGTGGTGCTAGATT+GGG 0.463771 3_2:+15440680 None:intergenic
AATCTAGCACCACTGATCAT+AGG 0.464495 3_2:-15440677 Msa0396690:CDS
AAAATTATATTAGACGATGA+TGG 0.467412 3_2:-15436524 Msa0396690:CDS
TGTTATTGAACTCAGCAATC+CGG 0.481393 3_2:-15440634 Msa0396690:CDS
ACCAAGTTATTTGAGGAACT+TGG 0.485648 3_2:-15438975 Msa0396690:CDS
AGATTCGGTGGTGAAAGAGT+AGG 0.488495 3_2:+15438235 None:intergenic
GCTAGTCTTGGAGTCATTGG+TGG 0.491588 3_2:-15437496 Msa0396690:CDS
AACACCCTTTGAAAATTTGA+GGG 0.491722 3_2:-15437521 Msa0396690:CDS
CCTTTATTATGGAATACTGT+TGG 0.492405 3_2:-15438951 Msa0396690:CDS
TCCAAGTTCCTCAAATAACT+TGG 0.522228 3_2:+15438974 None:intergenic
CACCCTCACCTCGTTTGTCG+AGG 0.530068 3_2:-15436418 Msa0396690:CDS
TGGCATCTCACCCTGATACA+AGG 0.537570 3_2:-15437965 Msa0396690:CDS
AGTCATTGGTGGCTCGATGA+AGG 0.546340 3_2:-15437485 Msa0396690:intron
ACAAGGATCTCATTTCTCAA+TGG 0.566776 3_2:-15437948 Msa0396690:intron
AAGCCTCGACAAACGAGGTG+AGG 0.569309 3_2:+15436415 None:intergenic
TGTTTGTCTAATATGCAAAA+CGG 0.578230 3_2:-15436692 Msa0396690:CDS
TGCTCTCTATGTGCTCTCCA+AGG 0.584461 3_2:-15440598 Msa0396690:intron
AGCCTCGACAAACGAGGTGA+GGG 0.588745 3_2:+15436416 None:intergenic
CTAGATTGGGTAGAATCACC+AGG 0.589884 3_2:+15440693 None:intergenic
GAAATGAGATCCTTGTATCA+GGG 0.596244 3_2:+15437955 None:intergenic
CACACTCGAGTTGATTGTGA+GGG 0.603583 3_2:+15438208 None:intergenic
TTTCGTGTAAGTACTTCTTG+TGG 0.613050 3_2:-15436196 Msa0396690:CDS
TAGCACCACTGATCATAGGA+TGG 0.627844 3_2:-15440673 Msa0396690:CDS
CTCGACAAACGAGGTGAGGG+TGG 0.635662 3_2:+15436419 None:intergenic
GATTGCTGAGTTCAATAACA+AGG 0.649486 3_2:+15440637 None:intergenic
GCACACTCGAGTTGATTGTG+AGG 0.665692 3_2:+15438207 None:intergenic
GTAAGCCATCCTATGATCAG+TGG 0.669763 3_2:+15440668 None:intergenic
ATCAAAAGCCTCGACAAACG+AGG 0.676514 3_2:+15436410 None:intergenic
TGTGAGGGAGAGAGATTCGG+TGG 0.689243 3_2:+15438223 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAAAAAAATATCACAA+CGG + chr3_2:15436553-15436572 None:intergenic 10.0%
!!! TTTTTTATAATTGAATTGAA+AGG - chr3_2:15436562-15436581 Msa0396690:intron 10.0%
!!! TTTTTATAATTGAATTGAAA+GGG - chr3_2:15436563-15436582 Msa0396690:intron 10.0%
!!! ATATTGTGATTTTTTTTTAA+TGG - chr3_2:15437028-15437047 Msa0396690:intron 10.0%
!! TAGAATAAAAAATTGATAAA+TGG + chr3_2:15437182-15437201 None:intergenic 10.0%
!!! AAAAAATTTAATGAAAGTTT+TGG + chr3_2:15437417-15437436 None:intergenic 10.0%
!! AAAAAGATGTATAATAAATA+GGG + chr3_2:15437602-15437621 None:intergenic 10.0%
!! GAAAAATAAAAACATAAATT+TGG + chr3_2:15438322-15438341 None:intergenic 10.0%
!!! GAATATTTTTTTTTTGTTTA+AGG - chr3_2:15438550-15438569 Msa0396690:intron 10.0%
!!! ATATATATTTTTTTACATCA+AGG + chr3_2:15438863-15438882 None:intergenic 10.0%
!! TGCATATAATAAAAAAAAAA+AGG + chr3_2:15439306-15439325 None:intergenic 10.0%
!! AGTTATAATAAATTAATTGT+CGG - chr3_2:15439798-15439817 Msa0396690:intron 10.0%
!!! TACAATAATATCTAATTTTA+GGG + chr3_2:15439852-15439871 None:intergenic 10.0%
!! ATTATATGAAAATTGATATT+AGG - chr3_2:15439950-15439969 Msa0396690:intron 10.0%
!! AATACTCTATAATAATAAAT+TGG + chr3_2:15440277-15440296 None:intergenic 10.0%
!!! TATACAGTAAAATTTTTCAA+TGG + chr3_2:15436955-15436974 None:intergenic 15.0%
!! AAAATCACAATATCAAATTT+CGG + chr3_2:15437021-15437040 None:intergenic 15.0%
!!! ACAATTTTTTTTCTTCAAAA+GGG + chr3_2:15437518-15437537 None:intergenic 15.0%
!! AAAGAAATAATTAATGTAGT+TGG + chr3_2:15437569-15437588 None:intergenic 15.0%
!! AAAAGATGTATAATAAATAG+GGG + chr3_2:15437601-15437620 None:intergenic 15.0%
!! GAAAAAGATGTATAATAAAT+AGG + chr3_2:15437603-15437622 None:intergenic 15.0%
!!! GAACTTTTATACTAAAATTT+CGG - chr3_2:15437830-15437849 Msa0396690:intron 15.0%
!! ATAGATTCATAATCTAAAAA+TGG + chr3_2:15438267-15438286 None:intergenic 15.0%
!!! TTGAATATTATTTTAGCATA+TGG - chr3_2:15438760-15438779 Msa0396690:intron 15.0%
!!! TAATAACGTGAAAAATATTT+TGG - chr3_2:15439157-15439176 Msa0396690:intron 15.0%
!!! AATAACGTGAAAAATATTTT+GGG - chr3_2:15439158-15439177 Msa0396690:intron 15.0%
!! TTAAAAAAACAAAAGTTAGA+AGG + chr3_2:15439446-15439465 None:intergenic 15.0%
!! TCCAAAAAAAAAAAACTTTA+TGG - chr3_2:15439682-15439701 Msa0396690:intron 15.0%
!!! AAATATCTAGTTATTTTTAG+CGG - chr3_2:15439706-15439725 Msa0396690:intron 15.0%
!! GACAATTAATTTATTATAAC+TGG + chr3_2:15439799-15439818 None:intergenic 15.0%
!!! CTACAATAATATCTAATTTT+AGG + chr3_2:15439853-15439872 None:intergenic 15.0%
!! AAAATTGATATTAGGTATTT+GGG - chr3_2:15439958-15439977 Msa0396690:intron 15.0%
!!! ATCATTTTTTGTTGAAAACT+TGG - chr3_2:15436906-15436925 Msa0396690:intron 20.0%
!!! AAATTGATAAATGGATTTCT+AGG + chr3_2:15437173-15437192 None:intergenic 20.0%
!!! GACAATTTTTTTTCTTCAAA+AGG + chr3_2:15437519-15437538 None:intergenic 20.0%
!!! AACATGTTATTTATTGTTGA+TGG + chr3_2:15437628-15437647 None:intergenic 20.0%
!!! GTATCATGTTTGTTTTATTT+TGG - chr3_2:15437808-15437827 Msa0396690:intron 20.0%
!!! ATTACTTATTATTTTCTAGC+TGG - chr3_2:15438011-15438030 Msa0396690:intron 20.0%
!!! AATTTAGTTTTTAAACCTCT+AGG - chr3_2:15438471-15438490 Msa0396690:intron 20.0%
!!! ATTGATTTTCATGTATTTTG+GGG + chr3_2:15438800-15438819 None:intergenic 20.0%
!!! GATTGATTTTCATGTATTTT+GGG + chr3_2:15438801-15438820 None:intergenic 20.0%
!!! TGATTGATTTTCATGTATTT+TGG + chr3_2:15438802-15438821 None:intergenic 20.0%
!!! ATGTTTTTTTTTATAGTGTG+TGG - chr3_2:15438912-15438931 Msa0396690:intron 20.0%
!! AGGAATGGATATAAATATAA+AGG + chr3_2:15439338-15439357 None:intergenic 20.0%
!!! TGTGAAAATATATTTTGCAA+TGG - chr3_2:15439539-15439558 Msa0396690:intron 20.0%
!! TTCACACAATAAACTAATTA+AGG - chr3_2:15439627-15439646 Msa0396690:intron 20.0%
!!! GCCATAAAGTTTTTTTTTTT+TGG + chr3_2:15439686-15439705 None:intergenic 20.0%
!!! CAATGTTAGTAAAAGTTTTA+AGG + chr3_2:15439770-15439789 None:intergenic 20.0%
!!! CTTAAAACTTTTACTAACAT+TGG - chr3_2:15439768-15439787 Msa0396690:intron 20.0%
!! GAAAATTGATATTAGGTATT+TGG - chr3_2:15439957-15439976 Msa0396690:intron 20.0%
!! AAAATTATATTAGACGATGA+TGG - chr3_2:15440373-15440392 Msa0396690:intron 20.0%
! GACAATTCAAAGATGTTATT+AGG + chr3_2:15436477-15436496 None:intergenic 25.0%
! GACAATTCAAAGATGTTATT+AGG + chr3_2:15436477-15436496 None:intergenic 25.0%
! TAATTGCTTGGTTATTGAAA+GGG - chr3_2:15436760-15436779 Msa0396690:intron 25.0%
! CAGATGCATTAAAATTGAAA+AGG + chr3_2:15436885-15436904 None:intergenic 25.0%
! TTTGTTGAAAACTTGGTATT+CGG - chr3_2:15436913-15436932 Msa0396690:intron 25.0%
!!! AAAATTTTTCAATGGTGTTC+CGG + chr3_2:15436947-15436966 None:intergenic 25.0%
! TTATTTATGTGTGCTCTTAA+AGG - chr3_2:15437145-15437164 Msa0396690:intron 25.0%
! ATTCAAATCTATCTTAAGAG+AGG + chr3_2:15437282-15437301 None:intergenic 25.0%
!! TCAAAAGGGTCTTATAATTA+GGG + chr3_2:15437504-15437523 None:intergenic 25.0%
!! TTCAAAAGGGTCTTATAATT+AGG + chr3_2:15437505-15437524 None:intergenic 25.0%
!!! ATTCCCGTTATTTTTTCAAA+AGG - chr3_2:15437739-15437758 Msa0396690:intron 25.0%
!!! TTCCCGTTATTTTTTCAAAA+GGG - chr3_2:15437740-15437759 Msa0396690:intron 25.0%
!!! TACTATTGCAGTATTTTTAC+AGG - chr3_2:15437969-15437988 Msa0396690:CDS 25.0%
! ATATTTGATGAAACACACAA+TGG - chr3_2:15438175-15438194 Msa0396690:intron 25.0%
!!! TGAAATGATGATTGTTTTGA+AGG + chr3_2:15438244-15438263 None:intergenic 25.0%
! AATTTGGAGAAAGATATACA+TGG + chr3_2:15438306-15438325 None:intergenic 25.0%
!!! TTATTTTTCCAACTTTTACG+AGG - chr3_2:15438333-15438352 Msa0396690:intron 25.0%
!! TATTTTTCCAACTTTTACGA+GGG - chr3_2:15438334-15438353 Msa0396690:intron 25.0%
! ACTTAGAAAATATATGGTGA+TGG - chr3_2:15438359-15438378 Msa0396690:intron 25.0%
!!! TTAGCTCAAGGATTTTTTTT+AGG - chr3_2:15438427-15438446 Msa0396690:intron 25.0%
!!! TAGCTCAAGGATTTTTTTTA+GGG - chr3_2:15438428-15438447 Msa0396690:intron 25.0%
! TTATTCTGAATTTCAATCCA+AGG - chr3_2:15439004-15439023 Msa0396690:intron 25.0%
!! GTTCTTTTGTGAAAATACTA+CGG - chr3_2:15439088-15439107 Msa0396690:intron 25.0%
! TACGGAGATATATAACTATT+TGG - chr3_2:15439106-15439125 Msa0396690:intron 25.0%
!!! TGAAAAATATTTTGGGTAAG+AGG - chr3_2:15439165-15439184 Msa0396690:intron 25.0%
!!! CTTTCCCATTTTTAACATAA+TGG + chr3_2:15439241-15439260 None:intergenic 25.0%
! AAAAACAAAAGTTAGAAGGA+TGG + chr3_2:15439442-15439461 None:intergenic 25.0%
! AAGGATTACTCTAAACTTTA+TGG - chr3_2:15439646-15439665 Msa0396690:intron 25.0%
!!! AAGTTTTAAGGACAGTTATA+AGG + chr3_2:15439758-15439777 None:intergenic 25.0%
!!! ATTGTCGGTAAAAGTTTTAA+CGG - chr3_2:15439813-15439832 Msa0396690:intron 25.0%
! CAAAACGAAATAAATAAGCA+TGG + chr3_2:15440112-15440131 None:intergenic 25.0%
!! TGCTTATTTATTTCGTTTTG+TGG - chr3_2:15440112-15440131 Msa0396690:intron 25.0%
! TGTTTGTCTAATATGCAAAA+CGG - chr3_2:15440205-15440224 Msa0396690:intron 25.0%
! TTCAACAATTCTTAAGATCA+TGG + chr3_2:15440561-15440580 None:intergenic 25.0%
! TAAGATGAAAGAAGTTTGAT+CGG + chr3_2:15440611-15440630 None:intergenic 25.0%
! AACTTCTTTCATCTTAAAAC+AGG - chr3_2:15440615-15440634 Msa0396690:CDS 25.0%
! ACTTCTTTCATCTTAAAACA+GGG - chr3_2:15440616-15440635 Msa0396690:CDS 25.0%
! ACGAAAAAAATTGATGAGAT+TGG + chr3_2:15440687-15440706 None:intergenic 25.0%
AGATAGATATGTAAGAACCT+TGG + chr3_2:15436319-15436338 None:intergenic 30.0%
!! TTCATTTTTAACGTCTCTCA+TGG - chr3_2:15436350-15436369 Msa0396690:intron 30.0%
!! TCATTTTTAACGTCTCTCAT+GGG - chr3_2:15436351-15436370 Msa0396690:intron 30.0%
TTCACAAGTAATCCAAATCT+TGG - chr3_2:15436375-15436394 Msa0396690:intron 30.0%
TGTTATTAGGTCTAGCAAAA+GGG + chr3_2:15436464-15436483 None:intergenic 30.0%
ATGTTATTAGGTCTAGCAAA+AGG + chr3_2:15436465-15436484 None:intergenic 30.0%
TGTTATTAGGTCTAGCAAAA+GGG + chr3_2:15436464-15436483 None:intergenic 30.0%
ATGTTATTAGGTCTAGCAAA+AGG + chr3_2:15436465-15436484 None:intergenic 30.0%
TCTGGGAGGTAAATAAAATT+TGG - chr3_2:15436655-15436674 Msa0396690:intron 30.0%
! CAAATTATTCTGGAGAACTT+GGG + chr3_2:15436709-15436728 None:intergenic 30.0%
! ACAAATTATTCTGGAGAACT+TGG + chr3_2:15436710-15436729 None:intergenic 30.0%
! TCTCCAGAATAATTTGTCTT+TGG - chr3_2:15436713-15436732 Msa0396690:CDS 30.0%
CTTTCAATAACCAAGCAATT+AGG + chr3_2:15436761-15436780 None:intergenic 30.0%
CTAATTGCTTGGTTATTGAA+AGG - chr3_2:15436759-15436778 Msa0396690:intron 30.0%
TTCACTGTTTAGTGTATCAT+GGG - chr3_2:15436783-15436802 Msa0396690:intron 30.0%
! TATAGCCTCATTTAAAGTGA+AGG - chr3_2:15436971-15436990 Msa0396690:intron 30.0%
ATCACAATATCAAATTTCGG+TGG + chr3_2:15437018-15437037 None:intergenic 30.0%
AAAAGTTGGATTACATGTGT+GGG + chr3_2:15437226-15437245 None:intergenic 30.0%
TTGTGATTAAGTCTGCTAAA+TGG + chr3_2:15437333-15437352 None:intergenic 30.0%
TTAGCAGACTTAATCACAAT+TGG - chr3_2:15437334-15437353 Msa0396690:intron 30.0%
! GCAAAGGAGTCTTATAAAAA+GGG + chr3_2:15437541-15437560 None:intergenic 30.0%
! AGCAAAGGAGTCTTATAAAA+AGG + chr3_2:15437542-15437561 None:intergenic 30.0%
AATGTAGTTGGAAATAGCAA+AGG + chr3_2:15437557-15437576 None:intergenic 30.0%
!! ATTTTGTCCTTTAAGAGAGA+GGG + chr3_2:15437666-15437685 None:intergenic 30.0%
!! AATTTTGTCCTTTAAGAGAG+AGG + chr3_2:15437667-15437686 None:intergenic 30.0%
! AATAACGGGAATTAAGATAG+TGG + chr3_2:15437731-15437750 None:intergenic 30.0%
CCAACAGTATTCCATAATAA+AGG + chr3_2:15437949-15437968 None:intergenic 30.0%
CCTTTATTATGGAATACTGT+TGG - chr3_2:15437946-15437965 Msa0396690:intron 30.0%
ATGAAACACACAATGGTATT+GGG - chr3_2:15438182-15438201 Msa0396690:intron 30.0%
AGGGAGACTTAGAAAATATA+TGG - chr3_2:15438353-15438372 Msa0396690:intron 30.0%
!!! GTTGTGCTGTTTTTTTTTTC+AGG - chr3_2:15438622-15438641 Msa0396690:intron 30.0%
CAGGCCATTATGTTAAAAAT+GGG - chr3_2:15439234-15439253 Msa0396690:intron 30.0%
!!! TTAACTTGCTTGTCGTTTTA+AGG + chr3_2:15439358-15439377 None:intergenic 30.0%
! TAACACCCTTTGAAAATTTG+AGG - chr3_2:15439375-15439394 Msa0396690:intron 30.0%
! AACACCCTTTGAAAATTTGA+GGG - chr3_2:15439376-15439395 Msa0396690:intron 30.0%
TGAACTAATCCTAACAAGTT+GGG + chr3_2:15439993-15440012 None:intergenic 30.0%
ATGAACTAATCCTAACAAGT+TGG + chr3_2:15439994-15440013 None:intergenic 30.0%
TTAGTTCATCTACATCATGA+AGG - chr3_2:15440005-15440024 Msa0396690:intron 30.0%
TAGTTCATCTACATCATGAA+GGG - chr3_2:15440006-15440025 Msa0396690:intron 30.0%
TTGTGGTATTTGATATTCAC+AGG - chr3_2:15440129-15440148 Msa0396690:intron 30.0%
ATAGAAACCTATGACTTCTT+TGG + chr3_2:15440161-15440180 None:intergenic 30.0%
GATATACAACTTTCATTAGC+AGG + chr3_2:15440325-15440344 None:intergenic 30.0%
ATTATAAATGTTGCAACCTG+TGG + chr3_2:15440349-15440368 None:intergenic 30.0%
! TATATTAGACGATGATGGTT+TGG - chr3_2:15440378-15440397 Msa0396690:intron 30.0%
!!! GCTACAACAGAGAATTTTTT+TGG - chr3_2:15440412-15440431 Msa0396690:intron 30.0%
CTCATTTATCTCAAAATTGC+AGG - chr3_2:15440521-15440540 Msa0396690:intron 30.0%
TTTATCTCAAAATTGCAGGT+TGG - chr3_2:15440525-15440544 Msa0396690:intron 30.0%
! AACGTTTGAAACAAGAGAAA+AGG + chr3_2:15440588-15440607 None:intergenic 30.0%
TAACAACTTATCTTCCAAAC+AGG - chr3_2:15440651-15440670 Msa0396690:CDS 30.0%
! ACATCGTTTATAGCTTTTCC+TGG - chr3_2:15436186-15436205 Msa0396690:CDS 35.0%
GATTGCTGAGTTCAATAACA+AGG + chr3_2:15436263-15436282 None:intergenic 35.0%
TGTTATTGAACTCAGCAATC+CGG - chr3_2:15436263-15436282 Msa0396690:CDS 35.0%
!!! AGAGCATTTTCTCTAAGATC+CGG + chr3_2:15436285-15436304 None:intergenic 35.0%
ACAAGTAATCCAAATCTTGG+AGG - chr3_2:15436378-15436397 Msa0396690:CDS 35.0%
AGGGTTCATTGTTATCACAA+CGG + chr3_2:15436499-15436518 None:intergenic 35.0%
GGAAATGAGACCTGATAATT+TGG - chr3_2:15436626-15436645 Msa0396690:intron 35.0%
! TGATAATTTGGAGTACGTCT+GGG - chr3_2:15436638-15436657 Msa0396690:intron 35.0%
AGAACTTGGGTTCAATTCTT+GGG + chr3_2:15436696-15436715 None:intergenic 35.0%
CCACCAAAGACAAATTATTC+TGG + chr3_2:15436719-15436738 None:intergenic 35.0%
! CCAGAATAATTTGTCTTTGG+TGG - chr3_2:15436716-15436735 Msa0396690:CDS 35.0%
GTTCACTGTTTAGTGTATCA+TGG - chr3_2:15436782-15436801 Msa0396690:intron 35.0%
AAAACTTGGTATTCGGTCAA+AGG - chr3_2:15436920-15436939 Msa0396690:intron 35.0%
CTTTGCCTTCACTTTAAATG+AGG + chr3_2:15436979-15436998 None:intergenic 35.0%
CAAAAGTTGGATTACATGTG+TGG + chr3_2:15437227-15437246 None:intergenic 35.0%
! CACACATGTAATCCAACTTT+TGG - chr3_2:15437225-15437244 Msa0396690:intron 35.0%
ATGGATATAGTCTGACAATG+CGG + chr3_2:15437314-15437333 None:intergenic 35.0%
! AAGGGTCTTATAATTAGGGA+CGG + chr3_2:15437500-15437519 None:intergenic 35.0%
!! GGCCCTTTTGAAAAAATAAC+GGG + chr3_2:15437745-15437764 None:intergenic 35.0%
!! GGGCCCTTTTGAAAAAATAA+CGG + chr3_2:15437746-15437765 None:intergenic 35.0%
TTCAAAAGGGCCCTATAATT+AGG - chr3_2:15437753-15437772 Msa0396690:intron 35.0%
TCAAAAGGGCCCTATAATTA+GGG - chr3_2:15437754-15437773 Msa0396690:intron 35.0%
ACCAAGTTATTTGAGGAACT+TGG - chr3_2:15437922-15437941 Msa0396690:intron 35.0%
TCCAAGTTCCTCAAATAACT+TGG + chr3_2:15437926-15437945 None:intergenic 35.0%
AGGAACTTGGACCTTTATTA+TGG - chr3_2:15437935-15437954 Msa0396690:intron 35.0%
TTGTGCATGTATCTTACTTG+TGG - chr3_2:15438134-15438153 Msa0396690:intron 35.0%
GATGAAACACACAATGGTAT+TGG - chr3_2:15438181-15438200 Msa0396690:intron 35.0%
GAAGGATTGTAATACGACAA+AGG + chr3_2:15438226-15438245 None:intergenic 35.0%
TGGAGAAAGATATACATGGT+AGG + chr3_2:15438302-15438321 None:intergenic 35.0%
TTTCAAGAGATGTTAGCTCA+AGG - chr3_2:15438415-15438434 Msa0396690:intron 35.0%
GATTTGAACTTCTTTCCTAG+AGG + chr3_2:15438489-15438508 None:intergenic 35.0%
!! GGTCATTTTACAGTTGTTTG+AGG + chr3_2:15438513-15438532 None:intergenic 35.0%
ATTCATAAGAGTCGCACTAA+TGG + chr3_2:15438534-15438553 None:intergenic 35.0%
CAATGTTCTAGCATTACTTC+AGG - chr3_2:15438712-15438731 Msa0396690:intron 35.0%
GAAATGAGATCCTTGTATCA+GGG + chr3_2:15438945-15438964 None:intergenic 35.0%
AGAAATGAGATCCTTGTATC+AGG + chr3_2:15438946-15438965 None:intergenic 35.0%
ACAAGGATCTCATTTCTCAA+TGG - chr3_2:15438949-15438968 Msa0396690:CDS 35.0%
TTGGGTAAGAGGAATGAATT+TGG - chr3_2:15439176-15439195 Msa0396690:intron 35.0%
GAATTTGGATTCTCTTTGAC+CGG - chr3_2:15439191-15439210 Msa0396690:intron 35.0%
AAGTTATTGCAACCAAAAGC+CGG + chr3_2:15439213-15439232 None:intergenic 35.0%
!! TTTTGGTTGCAATAACTTGC+AGG - chr3_2:15439215-15439234 Msa0396690:intron 35.0%
GCAGGCCATTATGTTAAAAA+TGG - chr3_2:15439233-15439252 Msa0396690:intron 35.0%
!!! TTGCTTGTCGTTTTAAGGAA+TGG + chr3_2:15439353-15439372 None:intergenic 35.0%
! GCAACCCTCAAATTTTCAAA+GGG + chr3_2:15439383-15439402 None:intergenic 35.0%
! AGCAACCCTCAAATTTTCAA+AGG + chr3_2:15439384-15439403 None:intergenic 35.0%
AGGACAGTTATAAGGAATGT+TGG + chr3_2:15439750-15439769 None:intergenic 35.0%
! ACTTACAAACCCAACTTGTT+AGG - chr3_2:15439981-15440000 Msa0396690:intron 35.0%
GTTAACACCAAAGAAGTCAT+AGG - chr3_2:15440151-15440170 Msa0396690:intron 35.0%
!! GCAGGTATTGGTAACAAAAA+TGG + chr3_2:15440307-15440326 None:intergenic 35.0%
CAACTTTCATTAGCAGGTAT+TGG + chr3_2:15440319-15440338 None:intergenic 35.0%
TGAAAGTTGTATATCTCCAC+AGG - chr3_2:15440330-15440349 Msa0396690:intron 35.0%
!!! TCGAGTTTTGAACATGATGT+TGG - chr3_2:15440441-15440460 Msa0396690:intron 35.0%
TTTCGTGTAAGTACTTCTTG+TGG - chr3_2:15440701-15440720 Msa0396690:CDS 35.0%
TTCTTTGTTGAGAGCGATTG+AGG + chr3_2:15436162-15436181 None:intergenic 40.0%
! ATGATCAGTGGTGCTAGATT+GGG + chr3_2:15436220-15436239 None:intergenic 40.0%
! TATGATCAGTGGTGCTAGAT+TGG + chr3_2:15436221-15436240 None:intergenic 40.0%
AATCTAGCACCACTGATCAT+AGG - chr3_2:15436220-15436239 Msa0396690:CDS 40.0%
! AAAGGGTTCATTGTTGGTAG+AGG + chr3_2:15436447-15436466 None:intergenic 40.0%
CTAGCAAAAGGGTTCATTGT+TGG + chr3_2:15436453-15436472 None:intergenic 40.0%
TGTTTACGCTTCACTTCTCA+GGG - chr3_2:15436604-15436623 Msa0396690:intron 40.0%
CAGACGTACTCCAAATTATC+AGG + chr3_2:15436639-15436658 None:intergenic 40.0%
! CTGATAATTTGGAGTACGTC+TGG - chr3_2:15436637-15436656 Msa0396690:intron 40.0%
GAGAACTTGGGTTCAATTCT+TGG + chr3_2:15436697-15436716 None:intergenic 40.0%
TTGGCAACTTCTCCAAAAGT+TGG + chr3_2:15437240-15437259 None:intergenic 40.0%
! AGCACGTTTAGTTTAACGCT+TGG + chr3_2:15437259-15437278 None:intergenic 40.0%
AAACCAACCCTCTCTCTTAA+AGG - chr3_2:15437656-15437675 Msa0396690:intron 40.0%
TACCTCCGTCCCTAATTATA+GGG + chr3_2:15437492-15437511 None:intergenic 40.0%
! TCAGAGCACCAAGTTATTTG+AGG - chr3_2:15437915-15437934 Msa0396690:intron 40.0%
TAAGTCTCCCTCGTAAAAGT+TGG + chr3_2:15438344-15438363 None:intergenic 40.0%
! AATTTGAGGGTTGCTAGTCT+TGG - chr3_2:15439389-15439408 Msa0396690:intron 40.0%
CTAGATTGGGTAGAATCACC+AGG + chr3_2:15436207-15436226 None:intergenic 45.0%
TAGCACCACTGATCATAGGA+TGG - chr3_2:15436224-15436243 Msa0396690:CDS 45.0%
GTAAGCCATCCTATGATCAG+TGG + chr3_2:15436232-15436251 None:intergenic 45.0%
ATCACAACGCCTCCAAGATT+TGG + chr3_2:15436390-15436409 None:intergenic 45.0%
GTGTTTACGCTTCACTTCTC+AGG - chr3_2:15436603-15436622 Msa0396690:intron 45.0%
GTTTACGCTTCACTTCTCAG+GGG - chr3_2:15436605-15436624 Msa0396690:intron 45.0%
! TAATTTGGAGTACGTCTGGG+AGG - chr3_2:15436641-15436660 Msa0396690:intron 45.0%
ACTTGGGTTCAATTCTTGGG+TGG + chr3_2:15436693-15436712 None:intergenic 45.0%
ACCACTTGAGCCTAATTGCT+TGG - chr3_2:15436748-15436767 Msa0396690:CDS 45.0%
ACCAAGCAATTAGGCTCAAG+TGG + chr3_2:15436752-15436771 None:intergenic 45.0%
TTGGTATTCGGTCAAAGGAC+CGG - chr3_2:15436925-15436944 Msa0396690:intron 45.0%
! GGTCTTATAATTAGGGACGG+AGG + chr3_2:15437497-15437516 None:intergenic 45.0%
! TGTCCTTTAAGAGAGAGGGT+TGG + chr3_2:15437662-15437681 None:intergenic 45.0%
AAGGGCCCTATAATTAGGGA+CGG - chr3_2:15437758-15437777 Msa0396690:intron 45.0%
CTACCTCCGTCCCTAATTAT+AGG + chr3_2:15437491-15437510 None:intergenic 45.0%
!! AGATTCGGTGGTGAAAGAGT+AGG + chr3_2:15438665-15438684 None:intergenic 45.0%
GATTGTGAGGGAGAGAGATT+CGG + chr3_2:15438680-15438699 None:intergenic 45.0%
!! CACACTCGAGTTGATTGTGA+GGG + chr3_2:15438692-15438711 None:intergenic 45.0%
!! CAACTTGTTACATGCAGCCT+TGG + chr3_2:15439024-15439043 None:intergenic 45.0%
!! GATTCTCTTTGACCGGCTTT+TGG - chr3_2:15439198-15439217 Msa0396690:intron 45.0%
!! GTTGCTAGTCTTGGAGTCAT+TGG - chr3_2:15439398-15439417 Msa0396690:intron 45.0%
ATCAAAAGCCTCGACAAACG+AGG + chr3_2:15440490-15440509 None:intergenic 45.0%
TTGGCATCTTTGAGCCTGTT+TGG + chr3_2:15440668-15440687 None:intergenic 45.0%
TGCTCTCTATGTGCTCTCCA+AGG - chr3_2:15436299-15436318 Msa0396690:intron 50.0%
!! GCACACTCGAGTTGATTGTG+AGG + chr3_2:15438693-15438712 None:intergenic 50.0%
TGGCATCTCACCCTGATACA+AGG - chr3_2:15438932-15438951 Msa0396690:CDS 50.0%
!! GCTAGTCTTGGAGTCATTGG+TGG - chr3_2:15439401-15439420 Msa0396690:intron 50.0%
! AGTCATTGGTGGCTCGATGA+AGG - chr3_2:15439412-15439431 Msa0396690:intron 50.0%
GGCCCTATAATTAGGGACGG+AGG - chr3_2:15437761-15437780 Msa0396690:intron 55.0%
TGTGAGGGAGAGAGATTCGG+TGG + chr3_2:15438677-15438696 None:intergenic 55.0%
AGCCTCGACAAACGAGGTGA+GGG + chr3_2:15440484-15440503 None:intergenic 55.0%
AAGCCTCGACAAACGAGGTG+AGG + chr3_2:15440485-15440504 None:intergenic 55.0%
CTCGACAAACGAGGTGAGGG+TGG + chr3_2:15440481-15440500 None:intergenic 60.0%
CACCCTCACCTCGTTTGTCG+AGG - chr3_2:15440479-15440498 Msa0396690:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 15436144 15440775 15436144 ID=Msa0396690;Name=Msa0396690
chr3_2 mRNA 15436144 15440775 15436144 ID=Msa0396690-mRNA-1;Parent=Msa0396690;Name=Msa0396690-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|8|0|304
chr3_2 exon 15440599 15440775 15440599 ID=Msa0396690-mRNA-1:exon:3448;Parent=Msa0396690-mRNA-1
chr3_2 exon 15438929 15439000 15438929 ID=Msa0396690-mRNA-1:exon:3447;Parent=Msa0396690-mRNA-1
chr3_2 exon 15438186 15438275 15438186 ID=Msa0396690-mRNA-1:exon:3446;Parent=Msa0396690-mRNA-1
chr3_2 exon 15437949 15437991 15437949 ID=Msa0396690-mRNA-1:exon:3445;Parent=Msa0396690-mRNA-1
chr3_2 exon 15437486 15437592 15437486 ID=Msa0396690-mRNA-1:exon:3444;Parent=Msa0396690-mRNA-1
chr3_2 exon 15436673 15436768 15436673 ID=Msa0396690-mRNA-1:exon:3443;Parent=Msa0396690-mRNA-1
chr3_2 exon 15436377 15436567 15436377 ID=Msa0396690-mRNA-1:exon:3442;Parent=Msa0396690-mRNA-1
chr3_2 exon 15436144 15436282 15436144 ID=Msa0396690-mRNA-1:exon:3441;Parent=Msa0396690-mRNA-1
chr3_2 CDS 15440599 15440775 15440599 ID=Msa0396690-mRNA-1:cds;Parent=Msa0396690-mRNA-1
chr3_2 CDS 15438929 15439000 15438929 ID=Msa0396690-mRNA-1:cds;Parent=Msa0396690-mRNA-1
chr3_2 CDS 15438186 15438275 15438186 ID=Msa0396690-mRNA-1:cds;Parent=Msa0396690-mRNA-1
chr3_2 CDS 15437949 15437991 15437949 ID=Msa0396690-mRNA-1:cds;Parent=Msa0396690-mRNA-1
chr3_2 CDS 15437486 15437592 15437486 ID=Msa0396690-mRNA-1:cds;Parent=Msa0396690-mRNA-1
chr3_2 CDS 15436673 15436768 15436673 ID=Msa0396690-mRNA-1:cds;Parent=Msa0396690-mRNA-1
chr3_2 CDS 15436377 15436567 15436377 ID=Msa0396690-mRNA-1:cds;Parent=Msa0396690-mRNA-1
chr3_2 CDS 15436144 15436282 15436144 ID=Msa0396690-mRNA-1:cds;Parent=Msa0396690-mRNA-1
Gene Sequence

>Msa0396690

ATGGCACACTTTACTCCTCAATCGCTCTCAACAAAGAACGATACATCGTTTATAGCTTTTCCTGGTGATTCTACCCAATCTAGCACCACTGATCATAGGATGGCTTACATGCAAAGCCTTGTTATTGAACTCAGCAATCCGGATCTTAGAGAAAATGCTCTCTATGTGCTCTCCAAGAGCACCAAGTTATTTGAGGAACTTGGACCTTTATTATGGAATACTGTTGGTACTATTGCAGTATTTTTACAGGAAATACTTTCAATATATCCTACTCTTTCACCACCGAATCTCTCTCCCTCACAATCAACTCGAGTGTGCAATGTTCTAGCATTACTTCAGTGTGTGGCATCTCACCCTGATACAAGGATCTCATTTCTCAATGCTAATATGCCTTTATATTTATATCCATTCCTTAAAACGACAAGCAAGTTAACACCCTTTGAAAATTTGAGGGTTGCTAGTCTTGGAGTCATTGGTGGCTCGATGAAGGTTAACACCAAAGAAGTCATAGGTTTCTATCTCTCAAGTGAGATAATTTCACTATGTTTGTCTAATATGCAAAACGGCTATGAATTGACAAAAAATGTTGCAACATTTATAATTCTGAAAATTATATTAGACGATGATGGTTTGGCTCGTGTTTGTGCTACAACAGAGAATTTTTTTGGAGTATGTCGAGTTTTGAACATGATGTTGGAAGATCTTGAGAATCCACCCTCACCTCGTTTGTCGAGGCTTTTGATTTCATGTTATTCTCATTTATCTCAAAATTGCAGGGCTAGAGTTGCATTAACAACTTATCTTCCAAACAGGCTCAAAGATGCCAATCTCATCAATTTTTTTCGTGTAAGTACTTCTTGTGGTATAAATATAAATTTGATTCAGAATTTGTTTGATACACCTAATATGATATAA
Protein sequence

>Msa0396690

MAHFTPQSLSTKNDTSFIAFPGDSTQSSTTDHRMAYMQSLVIELSNPDLRENALYVLSKSTKLFEELGPLLWNTVGTIAVFLQEILSIYPTLSPPNLSPSQSTRVCNVLALLQCVASHPDTRISFLNANMPLYLYPFLKTTSKLTPFENLRVASLGVIGGSMKVNTKEVIGFYLSSEIISLCLSNMQNGYELTKNVATFIILKIILDDDGLARVCATTENFFGVCRVLNMMLEDLENPPSPRLSRLLISCYSHLSQNCRARVALTTYLPNRLKDANLINFFRVSTSCGININLIQNLFDTPNMI*