AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0400820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0400820 MtrunA17_Chr2g0300441 82.627 236 41 0 1 236 1 236 1.86e-142 398
Msa0400820 MtrunA17_Chr7g0238991 79.386 228 46 1 10 236 21 248 2.18e-129 365
Msa0400820 MtrunA17_Chr8g0355091 71.730 237 66 1 1 236 1 237 5.76e-123 348
Msa0400820 MtrunA17_Chr2g0292791 71.233 219 63 0 1 219 1 219 4.70e-113 323
Msa0400820 MtrunA17_Chr1g0206721 71.689 219 62 0 1 219 1 219 1.55e-112 322
Msa0400820 MtrunA17_Chr1g0206701 69.333 225 69 0 1 225 1 225 9.39e-111 318
Msa0400820 MtrunA17_Chr1g0149391 70.270 222 66 0 1 222 1 222 8.29e-110 315
Msa0400820 MtrunA17_Chr2g0325971 68.085 235 57 3 1 225 1 227 1.02e-105 305
Msa0400820 MtrunA17_Chr1g0208561 65.957 235 62 3 1 225 1 227 1.61e-104 301
Msa0400820 MtrunA17_Chr3g0099421 67.580 219 71 0 1 219 26 244 7.11e-104 301
Msa0400820 MtrunA17_Chr3g0089141 65.957 235 62 3 1 225 1 227 2.23e-103 298
Msa0400820 MtrunA17_Chr1g0206691 65.909 220 74 1 18 236 1 220 3.46e-101 293
Msa0400820 MtrunA17_Chr1g0208581 64.831 236 63 3 1 225 1 227 1.06e-100 292
Msa0400820 MtrunA17_Chr2g0317451 60.593 236 92 1 1 235 1 236 1.07e-99 290
Msa0400820 MtrunA17_Chr2g0317481 60.593 236 92 1 1 235 1 236 4.21e-99 288
Msa0400820 MtrunA17_Chr1g0187361 62.222 225 85 0 1 225 1 225 7.80e-98 285
Msa0400820 MtrunA17_Chr5g0422421 61.778 225 86 0 1 225 1 225 1.01e-94 277
Msa0400820 MtrunA17_Chr1g0188671 60.444 225 89 0 1 225 39 263 1.72e-94 278
Msa0400820 MtrunA17_Chr5g0422941 58.228 237 98 1 1 236 1 237 2.05e-94 276
Msa0400820 MtrunA17_Chr5g0419601 59.111 225 92 0 1 225 1 225 4.04e-90 265
Msa0400820 MtrunA17_Chr1g0206991 70.166 181 54 0 1 181 1 181 5.08e-89 260
Msa0400820 MtrunA17_Chr3g0099411 62.557 219 63 2 1 219 1 200 1.07e-88 261
Msa0400820 MtrunA17_Chr3g0089151 84.848 132 20 0 1 132 1 132 5.60e-76 226
Msa0400820 MtrunA17_Chr2g0300421 70.149 134 40 0 1 134 1 134 8.34e-60 186
Msa0400820 MtrunA17_Chr3g0085241 44.828 174 96 0 3 176 35 208 8.86e-49 160
Msa0400820 MtrunA17_Chr3g0119901 49.342 152 77 0 9 160 9 160 1.69e-48 158
Msa0400820 MtrunA17_Chr3g0111931 55.882 102 45 0 21 122 2 103 3.74e-33 116
Msa0400820 MtrunA17_Chr8g0379021 36.723 177 102 2 5 178 8 177 3.08e-31 113
Msa0400820 MtrunA17_Chr2g0333981 36.196 163 100 1 5 167 8 166 5.57e-30 110
Msa0400820 MtrunA17_Chr1g0187351 72.727 66 18 0 18 83 1 66 7.29e-28 101
Msa0400820 MtrunA17_Chr3g0111841 68.116 69 22 0 16 84 3 71 4.03e-26 96.7
Msa0400820 MtrunA17_Chr4g0018951 41.304 138 69 3 22 159 25 150 8.17e-25 96.3
Msa0400820 MtrunA17_Chr4g0071601 34.104 173 100 3 5 167 8 176 2.12e-21 87.8
Msa0400820 MtrunA17_Chr3g0111831 61.290 62 24 0 15 76 10 71 1.38e-20 82.8
Msa0400820 MtrunA17_Chr1g0175601 43.564 101 55 1 9 109 2 100 9.71e-20 83.2
Msa0400820 MtrunA17_Chr1g0175611 51.282 78 38 0 9 86 2 79 1.96e-19 82.4
Msa0400820 MtrunA17_Chr1g0169761 53.623 69 32 0 9 77 2 70 2.51e-19 82.4
Msa0400820 MtrunA17_Chr8g0376821 53.623 69 32 0 9 77 2 70 5.06e-19 81.6
Msa0400820 MtrunA17_Chr4g0074101 37.324 142 64 4 9 125 2 143 1.13e-18 80.9
Msa0400820 MtrunA17_Chr1g0180591 41.584 101 57 1 9 109 2 100 4.64e-18 79.0
Msa0400820 MtrunA17_Chr8g0357771 50.725 69 34 0 9 77 2 70 2.44e-17 77.0
Msa0400820 MtrunA17_Chr4g0052061 38.182 110 66 1 9 118 2 109 9.12e-17 74.7
Msa0400820 MtrunA17_Chr8g0356071 50.725 69 34 0 9 77 2 70 1.20e-16 74.7
Msa0400820 MtrunA17_Chr4g0052081 49.296 71 36 0 9 79 2 72 1.71e-14 69.3
Msa0400820 MtrunA17_Chr3g0077501 44.118 68 38 0 9 76 2 69 3.16e-14 69.3
Msa0400820 MtrunA17_Chr4g0049901 47.692 65 34 0 10 74 29 93 3.58e-14 69.7
Msa0400820 MtrunA17_Chr5g0427401 44.118 68 38 0 9 76 2 69 1.96e-13 67.4
Msa0400820 MtrunA17_Chr3g0097101 36.364 110 52 1 3 112 11 102 4.01e-13 66.6
Msa0400820 MtrunA17_Chr4g0057151 36.842 95 60 0 10 104 3 97 5.91e-13 65.5
Msa0400820 MtrunA17_Chr5g0425191 50.943 53 26 0 9 61 2 54 6.00e-13 63.2
Msa0400820 MtrunA17_Chr5g0411691 29.577 213 110 8 9 186 2 209 7.64e-13 65.9
Msa0400820 MtrunA17_Chr8g0345001 31.013 158 96 3 8 159 17 167 7.73e-13 65.1
Msa0400820 MtrunA17_Chr3g0134451 45.714 70 37 1 9 77 2 71 1.02e-12 66.2
Msa0400820 MtrunA17_Chr8g0351051 44.286 70 37 1 7 76 2 69 1.16e-12 65.5
Msa0400820 MtrunA17_Chr2g0279821 41.176 68 40 0 9 76 2 69 1.25e-12 65.5
Msa0400820 MtrunA17_Chr4g0044071 47.368 57 30 0 9 65 2 58 1.95e-12 62.0
Msa0400820 MtrunA17_Chr4g0025531 24.171 211 133 6 1 204 1 191 1.96e-12 64.7
Msa0400820 MtrunA17_Chr8g0380021 30.723 166 92 5 9 158 18 176 2.20e-12 64.7
Msa0400820 MtrunA17_Chr2g0308251 41.096 73 43 0 9 81 2 74 2.49e-12 60.8
Msa0400820 MtrunA17_Chr5g0412461 39.706 68 41 0 9 76 2 69 3.01e-12 63.9
Msa0400820 MtrunA17_Chr6g0474371 40.476 84 44 3 7 86 2 83 3.27e-12 63.5
Msa0400820 MtrunA17_Chr5g0412271 39.706 68 41 0 9 76 2 69 3.68e-12 63.9
Msa0400820 MtrunA17_Chr2g0284911 42.647 68 39 0 9 76 17 84 3.85e-12 64.3
Msa0400820 MtrunA17_Chr4g0020221 36.585 82 52 0 9 90 2 83 4.68e-12 63.9
Msa0400820 MtrunA17_Chr4g0061691 42.254 71 38 2 7 76 2 70 5.79e-12 63.2
Msa0400820 MtrunA17_Chr1g0159661 44.776 67 36 1 9 75 2 67 5.85e-12 62.4
Msa0400820 MtrunA17_Chr5g0404951 45.714 70 36 1 9 76 2 71 6.07e-12 63.2
Msa0400820 MtrunA17_Chr1g0173161 44.262 61 34 0 9 69 2 62 7.39e-12 59.7
Msa0400820 MtrunA17_Chr5g0427921 42.105 57 33 0 9 65 2 58 1.09e-11 58.5
Msa0400820 MtrunA17_Chr8g0351041 27.160 162 102 3 10 167 3 152 1.09e-11 61.2
Msa0400820 MtrunA17_Chr3g0125241 44.776 67 36 1 9 75 27 92 1.44e-11 61.6
Msa0400820 MtrunA17_Chr4g0049541 39.706 68 41 0 9 76 2 69 1.53e-11 62.0
Msa0400820 MtrunA17_Chr1g0204111 41.176 68 40 0 9 76 18 85 2.91e-11 61.6
Msa0400820 MtrunA17_Chr3g0142191 42.857 63 36 0 9 71 2 64 3.46e-11 61.2
Msa0400820 MtrunA17_Chr6g0455511 35.165 91 55 2 9 95 2 92 3.98e-11 61.2
Msa0400820 MtrunA17_Chr7g0246381 40.909 66 34 1 9 69 2 67 5.29e-11 57.0
Msa0400820 MtrunA17_Chr5g0420081 44.643 56 31 0 9 64 2 57 6.93e-11 60.5
Msa0400820 MtrunA17_Chr7g0246431 40.299 67 40 0 10 76 3 69 8.51e-11 59.7
Msa0400820 MtrunA17_Chr8g0377561 44.643 56 31 0 9 64 2 57 8.57e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0400820 AT5G60440.1 50.000 168 81 2 4 168 2 169 3.40e-47 158
Msa0400820 AT2G24840.1 44.654 159 81 3 9 163 63 218 6.07e-35 125
Msa0400820 AT1G01530.1 43.373 166 87 3 4 166 2 163 2.39e-34 123
Msa0400820 AT1G65360.1 34.956 226 123 6 4 211 2 221 3.49e-33 120
Msa0400820 AT4G36590.1 39.759 166 88 4 4 160 2 164 5.75e-29 109
Msa0400820 AT3G04100.1 29.341 167 104 4 2 159 7 168 1.43e-19 84.0
Msa0400820 AT3G66656.1 37.705 122 66 2 9 123 2 120 6.54e-19 81.6
Msa0400820 AT2G34440.1 37.168 113 70 1 9 121 2 113 9.54e-18 78.2
Msa0400820 AT1G72350.1 50.685 73 34 1 9 81 43 113 1.42e-17 79.0
Msa0400820 AT1G28460.1 37.755 98 61 0 5 102 4 101 5.78e-15 70.9
Msa0400820 AT4G09960.2 39.535 86 51 1 9 93 2 87 1.68e-14 70.5
Msa0400820 AT4G09960.6 39.535 86 51 1 9 93 3 88 1.96e-14 70.5
Msa0400820 AT4G09960.1 39.535 86 51 1 9 93 2 87 1.99e-14 70.5
Msa0400820 AT4G09960.5 39.535 86 51 1 9 93 12 97 2.39e-14 70.5
Msa0400820 AT4G09960.3 40.000 90 51 2 5 93 26 113 2.87e-14 70.5
Msa0400820 AT2G45660.2 45.588 68 37 0 10 77 3 70 3.94e-14 68.2
Msa0400820 AT4G09960.4 40.000 90 51 2 5 93 95 182 5.90e-14 70.5
Msa0400820 AT4G18960.1 32.759 116 76 1 2 115 11 126 8.01e-14 69.3
Msa0400820 AT2G45660.1 45.588 68 37 0 10 77 3 70 8.03e-14 68.6
Msa0400820 AT3G54340.1 44.286 70 37 1 9 76 2 71 1.24e-13 68.6
Msa0400820 AT2G45650.1 42.647 68 39 0 9 76 2 69 2.59e-13 67.8
Msa0400820 AT1G77980.1 44.286 70 38 1 9 77 2 71 3.48e-13 68.2
Msa0400820 AT1G22130.1 35.870 92 58 1 9 99 2 93 7.20e-13 67.4
Msa0400820 AT3G57230.2 39.706 68 41 0 9 76 2 69 7.66e-13 64.3
Msa0400820 AT2G22540.3 42.647 68 39 0 9 76 2 69 8.41e-13 66.2
Msa0400820 AT2G22540.1 42.647 68 39 0 9 76 2 69 8.41e-13 66.2
Msa0400820 AT4G22950.2 43.284 67 38 0 10 76 3 69 9.45e-13 64.7
Msa0400820 AT5G51870.2 46.269 67 36 0 10 76 3 69 1.22e-12 64.3
Msa0400820 AT5G51870.1 46.269 67 36 0 10 76 3 69 1.35e-12 65.1
Msa0400820 AT4G22950.3 43.284 67 38 0 10 76 3 69 1.62e-12 65.1
Msa0400820 AT4G22950.1 43.284 67 38 0 10 76 3 69 1.62e-12 65.1
Msa0400820 AT2G42830.1 42.667 75 41 1 2 76 12 84 1.72e-12 65.5
Msa0400820 AT2G42830.2 42.667 75 41 1 2 76 12 84 1.77e-12 65.5
Msa0400820 AT5G51870.4 46.269 67 36 0 10 76 3 69 2.00e-12 64.7
Msa0400820 AT3G58780.1 42.667 75 41 1 2 76 12 84 2.04e-12 65.1
Msa0400820 AT5G51870.3 46.269 67 36 0 10 76 13 79 2.06e-12 65.1
Msa0400820 AT3G58780.5 42.667 75 41 1 2 76 18 90 2.19e-12 65.1
Msa0400820 AT4G37940.4 41.176 68 40 0 9 76 2 69 2.25e-12 64.7
Msa0400820 AT1G77950.3 39.474 76 44 2 9 83 2 76 2.35e-12 65.1
Msa0400820 AT1G77950.2 39.474 76 44 2 9 83 2 76 2.35e-12 65.1
Msa0400820 AT1G77950.1 39.474 76 44 2 9 83 2 76 2.35e-12 65.1
Msa0400820 AT1G77950.4 39.474 76 44 2 9 83 2 76 2.35e-12 65.1
Msa0400820 AT1G29962.1 34.653 101 63 1 5 102 4 104 2.35e-12 63.9
Msa0400820 AT4G24540.1 42.647 68 39 0 9 76 2 69 2.60e-12 64.3
Msa0400820 AT1G17310.1 32.934 167 71 4 8 169 47 177 2.70e-12 64.3
Msa0400820 AT4G37940.1 41.176 68 40 0 9 76 2 69 2.77e-12 64.7
Msa0400820 AT4G37940.3 41.176 68 40 0 9 76 2 69 2.87e-12 63.5
Msa0400820 AT1G17310.2 32.738 168 72 4 8 170 58 189 3.15e-12 64.3
Msa0400820 AT5G51870.5 46.269 67 36 0 10 76 26 92 3.28e-12 63.9
Msa0400820 AT3G58780.4 42.667 75 41 1 2 76 12 84 3.55e-12 65.1
Msa0400820 AT3G57230.1 39.706 68 41 0 9 76 2 69 3.61e-12 64.3
Msa0400820 AT3G57230.4 39.706 68 41 0 9 76 2 69 3.61e-12 64.3
Msa0400820 AT5G10140.2 43.478 69 38 1 9 76 2 70 3.95e-12 63.2
Msa0400820 AT5G13790.2 44.118 68 38 0 9 76 2 69 4.38e-12 64.7
Msa0400820 AT5G13790.1 44.118 68 38 0 9 76 2 69 4.39e-12 64.7
Msa0400820 AT5G10140.4 43.478 69 38 1 9 76 2 70 4.61e-12 63.2
Msa0400820 AT5G10140.1 43.478 69 38 1 9 76 2 70 4.98e-12 63.2
Msa0400820 AT3G61120.1 44.118 68 38 0 9 76 2 69 5.02e-12 63.9
Msa0400820 AT5G62165.14 41.791 67 39 0 10 76 3 69 5.92e-12 60.8
Msa0400820 AT1G26310.1 45.070 71 36 2 7 76 2 70 6.09e-12 63.9
Msa0400820 AT5G62165.17 41.791 67 39 0 10 76 3 69 7.06e-12 62.0
Msa0400820 AT5G62165.12 41.791 67 39 0 10 76 3 69 7.06e-12 62.0
Msa0400820 AT5G62165.11 41.791 67 39 0 10 76 3 69 7.06e-12 62.0
Msa0400820 AT5G62165.6 41.791 67 39 0 10 76 3 69 7.83e-12 62.0
Msa0400820 AT5G62165.9 41.791 67 39 0 10 76 3 69 7.83e-12 62.0
Msa0400820 AT5G62165.7 41.791 67 39 0 10 76 3 69 7.83e-12 62.0
Msa0400820 AT5G62165.8 41.791 67 39 0 10 76 3 69 7.83e-12 62.0
Msa0400820 AT5G62165.13 41.791 67 39 0 10 76 3 69 7.83e-12 62.0
Msa0400820 AT5G10140.3 43.478 69 38 1 9 76 2 70 7.91e-12 62.4
Msa0400820 AT2G22540.2 48.214 56 29 0 9 64 2 57 8.70e-12 63.2
Msa0400820 AT3G57390.3 41.176 68 40 0 9 76 2 69 1.05e-11 62.8
Msa0400820 AT5G62165.10 41.791 67 39 0 10 76 3 69 2.15e-11 61.6
Msa0400820 AT5G62165.4 41.791 67 39 0 10 76 3 69 2.15e-11 61.6
Msa0400820 AT5G62165.3 41.791 67 39 0 10 76 3 69 2.15e-11 61.6
Msa0400820 AT5G62165.1 41.791 67 39 0 10 76 3 69 2.15e-11 61.6
Msa0400820 AT5G62165.2 41.791 67 39 0 10 76 3 69 2.15e-11 61.6
Msa0400820 AT3G57390.1 41.176 68 40 0 9 76 2 69 2.26e-11 62.4
Msa0400820 AT2G22630.1 39.706 68 41 0 9 76 2 69 2.69e-11 61.6
Msa0400820 AT2G22630.2 39.706 68 41 0 9 76 2 69 2.69e-11 61.6
Msa0400820 AT5G51860.2 43.284 67 38 0 10 76 3 69 2.98e-11 61.2
Msa0400820 AT3G30260.1 39.130 69 41 1 9 76 2 70 3.34e-11 61.6
Msa0400820 AT1G28450.1 25.143 175 119 4 5 168 4 177 3.72e-11 60.8
Msa0400820 AT5G51860.1 43.284 67 38 0 10 76 3 69 3.83e-11 61.2
Msa0400820 AT5G20240.2 41.429 70 39 1 9 76 2 71 5.90e-11 59.3
Msa0400820 AT5G60910.1 41.892 74 39 1 9 78 2 75 7.52e-11 60.8
Msa0400820 AT5G20240.1 41.429 70 39 1 9 76 2 71 7.97e-11 60.1
Msa0400820 AT3G02310.2 48.214 56 29 0 9 64 2 57 8.90e-11 60.1

Find 50 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTATTCAAGAAGATTT+TGG 0.197704 3_2:-25543309 Msa0400820:CDS
TTTAATTGGCTTCAACAATA+TGG 0.202381 3_2:-25543099 Msa0400820:CDS
TCATAGATTGCTATCTCTTT+GGG 0.241640 3_2:-25543538 Msa0400820:CDS
ATCTCAGTTTGTTGAGGATT+TGG 0.255732 3_2:+25543164 None:intergenic
ATGTTGCAACCTCATTTAAT+TGG 0.256996 3_2:-25543113 Msa0400820:CDS
AAACCATTACCAACAGAAAA+TGG 0.261012 3_2:+25543215 None:intergenic
GTCATAGATTGCTATCTCTT+TGG 0.261827 3_2:-25543539 Msa0400820:CDS
TGGTGGGCTTGTCCGATTAA+TGG 0.300675 3_2:-25543350 Msa0400820:CDS
AATATGGGAGGAGGAGGATA+TGG 0.331968 3_2:-25543083 Msa0400820:CDS
AGGCGTTCAAGGCTCAATTT+TGG 0.334747 3_2:-25543370 Msa0400820:CDS
GGTGGGCTTGTCCGATTAAT+GGG 0.369852 3_2:-25543349 Msa0400820:CDS
GTCCGATTAATGGGATGAAT+AGG 0.377904 3_2:-25543340 Msa0400820:CDS
GCCCTATTCATCCCATTAAT+CGG 0.382765 3_2:+25543338 None:intergenic
TTAATTGGCTTCAACAATAT+GGG 0.383325 3_2:-25543098 Msa0400820:CDS
TTGTTGCTCATCATGTTGAT+AGG 0.402553 3_2:-25543271 Msa0400820:CDS
GTGAGCCTCAATGAATTCTA+TGG 0.413803 3_2:+25543493 None:intergenic
ATAATAGAGAGGGATGTTAG+AGG 0.415435 3_2:+25543190 None:intergenic
TCCGATTAATGGGATGAATA+GGG 0.426477 3_2:-25543339 Msa0400820:CDS
ATTAAATGAGGTTGCAACAT+AGG 0.428043 3_2:+25543116 None:intergenic
TCTAGAATTTCAATAGAATC+CGG 0.430673 3_2:+25543055 None:intergenic
TCTATGACCGTATCAACATT+TGG 0.448129 3_2:+25543554 None:intergenic
ACCTTTCCTTGCGCTTGACA+TGG 0.477663 3_2:+25543754 None:intergenic
AGCATTGAGCTCACACACTT+TGG 0.483043 3_2:+25543463 None:intergenic
TTAAATGAGGTTGCAACATA+GGG 0.493554 3_2:+25543117 None:intergenic
CTAGAATTTCAATAGAATCC+GGG 0.508602 3_2:+25543056 None:intergenic
CGGTCATCCAAATGTTGATA+CGG 0.514501 3_2:-25543561 Msa0400820:CDS
AGAAACATCTACACCACAAA+GGG 0.515799 3_2:+25543622 None:intergenic
TGTATTCTCACCTAGTGAAA+AGG 0.518205 3_2:-25543594 Msa0400820:CDS
GTTGATAGGAGTGCAATTCA+AGG 0.523826 3_2:-25543257 Msa0400820:CDS
TTCAACAATATGGGAGGAGG+AGG 0.541923 3_2:-25543089 Msa0400820:CDS
GCTCACACACTTTGGCGCTA+CGG 0.546232 3_2:+25543471 None:intergenic
TTGTTGAAGCCAATTAAATG+AGG 0.551491 3_2:+25543104 None:intergenic
GAAAACATCTCAGTTTGTTG+AGG 0.554882 3_2:+25543158 None:intergenic
GCTTTGAGAAAATCACTTGG+AGG 0.556997 3_2:+25543684 None:intergenic
GGCTTCAACAATATGGGAGG+AGG 0.561767 3_2:-25543092 Msa0400820:CDS
GGATTTGGCTGATAATAGAG+AGG 0.582271 3_2:+25543179 None:intergenic
TAGAATTTCAATAGAATCCG+GGG 0.585223 3_2:+25543057 None:intergenic
AATCAACAACACATCGGATG+AGG 0.592418 3_2:-25543429 Msa0400820:CDS
ACCATGTCAAGCGCAAGGAA+AGG 0.599500 3_2:-25543755 Msa0400820:CDS
GATTTGGCTGATAATAGAGA+GGG 0.600199 3_2:+25543180 None:intergenic
ATTGGCTTCAACAATATGGG+AGG 0.605743 3_2:-25543095 Msa0400820:CDS
CATAGATTGCTATCTCTTTG+GGG 0.615069 3_2:-25543537 Msa0400820:CDS
GACGCTTTGAGAAAATCACT+TGG 0.615395 3_2:+25543681 None:intergenic
CTTCAACCATGTCAAGCGCA+AGG 0.629141 3_2:-25543760 None:intergenic
TCAAGCGCAAGGAAAGGTCG+TGG 0.632671 3_2:-25543749 Msa0400820:CDS
AGAATTTCAATAGAATCCGG+GGG 0.639236 3_2:+25543058 None:intergenic
GAGAAACATCTACACCACAA+AGG 0.651442 3_2:+25543621 None:intergenic
GATTCAAATCAACAACACAT+CGG 0.687185 3_2:-25543435 Msa0400820:CDS
TAGCACCATAGAATTCATTG+AGG 0.695216 3_2:-25543498 Msa0400820:CDS
GAATTTCAATAGAATCCGGG+GGG 0.765426 3_2:+25543059 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTATTCAAGAAGATTT+TGG - chr3_2:25543507-25543526 Msa0400820:CDS 20.0%
!! AAAGAAAATACCTTTTCACT+AGG + chr3_2:25543235-25543254 None:intergenic 25.0%
!!! AGTGAAAAGGTATTTTCTTT+CGG - chr3_2:25543235-25543254 Msa0400820:CDS 25.0%
!! ATTATTCAAGAAGATTTTGG+TGG - chr3_2:25543510-25543529 Msa0400820:CDS 25.0%
!! CAAATTTTTCCATTTTCTGT+TGG - chr3_2:25543592-25543611 Msa0400820:CDS 25.0%
! ATAGGGTTATGAAAAATTTG+TGG + chr3_2:25543685-25543704 None:intergenic 25.0%
!! TTTAATTGGCTTCAACAATA+TGG - chr3_2:25543717-25543736 Msa0400820:CDS 25.0%
!! TTAATTGGCTTCAACAATAT+GGG - chr3_2:25543718-25543737 Msa0400820:CDS 25.0%
!!! TTTTTTTCGTCTCGATCTTT+TGG + chr3_2:25543096-25543115 None:intergenic 30.0%
TCATAGATTGCTATCTCTTT+GGG - chr3_2:25543278-25543297 Msa0400820:CDS 30.0%
GATTCAAATCAACAACACAT+CGG - chr3_2:25543381-25543400 Msa0400820:CDS 30.0%
!! TTTCCATTTTCTGTTGGTAA+TGG - chr3_2:25543598-25543617 Msa0400820:CDS 30.0%
AAACCATTACCAACAGAAAA+TGG + chr3_2:25543604-25543623 None:intergenic 30.0%
TTAAATGAGGTTGCAACATA+GGG + chr3_2:25543702-25543721 None:intergenic 30.0%
ATTAAATGAGGTTGCAACAT+AGG + chr3_2:25543703-25543722 None:intergenic 30.0%
ATGTTGCAACCTCATTTAAT+TGG - chr3_2:25543703-25543722 Msa0400820:CDS 30.0%
TTGTTGAAGCCAATTAAATG+AGG + chr3_2:25543715-25543734 None:intergenic 30.0%
AGAAACATCTACACCACAAA+GGG + chr3_2:25543197-25543216 None:intergenic 35.0%
TGTATTCTCACCTAGTGAAA+AGG - chr3_2:25543222-25543241 Msa0400820:CDS 35.0%
TCTATGACCGTATCAACATT+TGG + chr3_2:25543265-25543284 None:intergenic 35.0%
GTCATAGATTGCTATCTCTT+TGG - chr3_2:25543277-25543296 Msa0400820:CDS 35.0%
CATAGATTGCTATCTCTTTG+GGG - chr3_2:25543279-25543298 Msa0400820:CDS 35.0%
!!! TCTATGGTGCTATTGTTTTG+CGG + chr3_2:25543310-25543329 None:intergenic 35.0%
TAGCACCATAGAATTCATTG+AGG - chr3_2:25543318-25543337 Msa0400820:CDS 35.0%
TCCGATTAATGGGATGAATA+GGG - chr3_2:25543477-25543496 Msa0400820:CDS 35.0%
TTGTTGCTCATCATGTTGAT+AGG - chr3_2:25543545-25543564 Msa0400820:CDS 35.0%
ATAATAGAGAGGGATGTTAG+AGG + chr3_2:25543629-25543648 None:intergenic 35.0%
GATTTGGCTGATAATAGAGA+GGG + chr3_2:25543639-25543658 None:intergenic 35.0%
!! ATCTCAGTTTGTTGAGGATT+TGG + chr3_2:25543655-25543674 None:intergenic 35.0%
!! GAAAACATCTCAGTTTGTTG+AGG + chr3_2:25543661-25543680 None:intergenic 35.0%
!! GCTTTGAGAAAATCACTTGG+AGG + chr3_2:25543135-25543154 None:intergenic 40.0%
!! GACGCTTTGAGAAAATCACT+TGG + chr3_2:25543138-25543157 None:intergenic 40.0%
!!! ATTTTCTCAAAGCGTCGTAG+CGG - chr3_2:25543142-25543161 Msa0400820:CDS 40.0%
GAGAAACATCTACACCACAA+AGG + chr3_2:25543198-25543217 None:intergenic 40.0%
CGGTCATCCAAATGTTGATA+CGG - chr3_2:25543255-25543274 Msa0400820:CDS 40.0%
GTGAGCCTCAATGAATTCTA+TGG + chr3_2:25543326-25543345 None:intergenic 40.0%
AATCAACAACACATCGGATG+AGG - chr3_2:25543387-25543406 Msa0400820:CDS 40.0%
!! TGAGTTGAGTCTTTTGTGCA+AGG - chr3_2:25543426-25543445 Msa0400820:CDS 40.0%
!! GTTCAAGGCTCAATTTTGGT+GGG - chr3_2:25543450-25543469 Msa0400820:CDS 40.0%
GTCCGATTAATGGGATGAAT+AGG - chr3_2:25543476-25543495 Msa0400820:CDS 40.0%
GCCCTATTCATCCCATTAAT+CGG + chr3_2:25543481-25543500 None:intergenic 40.0%
! GTTGATAGGAGTGCAATTCA+AGG - chr3_2:25543559-25543578 Msa0400820:CDS 40.0%
GGATTTGGCTGATAATAGAG+AGG + chr3_2:25543640-25543659 None:intergenic 40.0%
! ATTGGCTTCAACAATATGGG+AGG - chr3_2:25543721-25543740 Msa0400820:CDS 40.0%
!!! TTTTCTCAAAGCGTCGTAGC+GGG - chr3_2:25543143-25543162 Msa0400820:CDS 45.0%
! AGTGAGTTTTGCTCCCTTTG+TGG - chr3_2:25543181-25543200 Msa0400820:CDS 45.0%
!!! TGGTGCTATTGTTTTGCGGT+GGG + chr3_2:25543306-25543325 None:intergenic 45.0%
!!! ATGGTGCTATTGTTTTGCGG+TGG + chr3_2:25543307-25543326 None:intergenic 45.0%
! AGCATTGAGCTCACACACTT+TGG + chr3_2:25543356-25543375 None:intergenic 45.0%
TCGGATGAGGAGAAAAAGCA+TGG - chr3_2:25543400-25543419 Msa0400820:CDS 45.0%
!! TCTTTTGTGCAAGGCGTTCA+AGG - chr3_2:25543435-25543454 Msa0400820:CDS 45.0%
! AGGCGTTCAAGGCTCAATTT+TGG - chr3_2:25543446-25543465 Msa0400820:CDS 45.0%
! CGTTCAAGGCTCAATTTTGG+TGG - chr3_2:25543449-25543468 Msa0400820:CDS 45.0%
TTCAACAATATGGGAGGAGG+AGG - chr3_2:25543727-25543746 Msa0400820:CDS 45.0%
AATATGGGAGGAGGAGGATA+TGG - chr3_2:25543733-25543752 Msa0400820:CDS 45.0%
!! TGGTGGGCTTGTCCGATTAA+TGG - chr3_2:25543466-25543485 Msa0400820:CDS 50.0%
! GGTGGGCTTGTCCGATTAAT+GGG - chr3_2:25543467-25543486 Msa0400820:CDS 50.0%
GGCTTCAACAATATGGGAGG+AGG - chr3_2:25543724-25543743 Msa0400820:CDS 50.0%
TCAAGCGCAAGGAAAGGTCG+TGG - chr3_2:25543067-25543086 Msa0400820:CDS 55.0%
GCTCACACACTTTGGCGCTA+CGG + chr3_2:25543348-25543367 None:intergenic 55.0%
GGAGGAGGATATGGACCCCC+CGG - chr3_2:25543742-25543761 Msa0400820:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 25543064 25543774 25543064 ID=Msa0400820;Name=Msa0400820
chr3_2 mRNA 25543064 25543774 25543064 ID=Msa0400820-mRNA-1;Parent=Msa0400820;Name=Msa0400820-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|236
chr3_2 exon 25543064 25543774 25543064 ID=Msa0400820-mRNA-1:exon:5316;Parent=Msa0400820-mRNA-1
chr3_2 CDS 25543064 25543774 25543064 ID=Msa0400820-mRNA-1:cds;Parent=Msa0400820-mRNA-1
Gene Sequence

>Msa0400820

ATGTCAAGCGCAAGGAAAGGTCGTGGTCGCCAAAAGATCGAGACGAAAAAAATGAGCAACGAGAGCAACCTCCAAGTGATTTTCTCAAAGCGTCGTAGCGGGCTCTTCAAGAAAGTCAGTGAGTTTTGCTCCCTTTGTGGTGTAGATGTTTCTCTCGTTGTATTCTCACCTAGTGAAAAGGTATTTTCTTTCGGTCATCCAAATGTTGATACGGTCATAGATTGCTATCTCTTTGGGGTCCCACCGCAAAACAATAGCACCATAGAATTCATTGAGGCTCACCGTAGCGCCAAAGTGTGTGAGCTCAATGCTGAGTTGATTCAAATCAACAACACATCGGATGAGGAGAAAAAGCATGGTGATGAGTTGAGTCTTTTGTGCAAGGCGTTCAAGGCTCAATTTTGGTGGGCTTGTCCGATTAATGGGATGAATAGGGCTCAACTTGAATTATTCAAGAAGATTTTGGTGGAGCTTAAGAAACTTGTTGCTCATCATGTTGATAGGAGTGCAATTCAAGGTGCTTTTGCTCAAATTTTTCCATTTTCTGTTGGTAATGGTTTATCCTCTAACATCCCTCTCTATTATCAGCCAAATCCTCAACAAACTGAGATGTTTTCGCCACAAATTTTTCATAACCCTATGTTGCAACCTCATTTAATTGGCTTCAACAATATGGGAGGAGGAGGATATGGACCCCCCGGATTCTATTGA
Protein sequence

>Msa0400820

MSSARKGRGRQKIETKKMSNESNLQVIFSKRRSGLFKKVSEFCSLCGVDVSLVVFSPSEKVFSFGHPNVDTVIDCYLFGVPPQNNSTIEFIEAHRSAKVCELNAELIQINNTSDEEKKHGDELSLLCKAFKAQFWWACPINGMNRAQLELFKKILVELKKLVAHHVDRSAIQGAFAQIFPFSVGNGLSSNIPLYYQPNPQQTEMFSPQIFHNPMLQPHLIGFNNMGGGGYGPPGFY*