AlfalfaGEDB | Msa0402430

M. sativa cultivar zhongmu-4 / Msa0402430

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0402430	XP_039685680.1	76.537	797	83	6	1	724	35	800	0.0	1196

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0402430	sp\|Q9SZL8\|FRS5_ARATH	25.676	666	349	22	16	635	72	637	9.77e-47	181

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0402430	D3YBB9	66.911	819	154	7	3	743	69	848	0.0	1076

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TFs/TRs:

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Gene ID	Type	Classification
Msa0402430	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0402430	MtrunA17_Chr3g0088371	65.946	185	34	2	588	743	1	185	4.86e-70	227
Msa0402430	MtrunA17_Chr2g0324011	26.316	665	352	15	13	635	3	571	1.02e-69	239
Msa0402430	MtrunA17_Chr3g0082121	26.917	665	334	16	13	632	33	590	5.98e-63	223
Msa0402430	MtrunA17_Chr1g0202341	26.415	636	373	18	16	632	50	609	5.18e-58	209
Msa0402430	MtrunA17_Chr8g0363841	25.868	634	375	17	22	635	22	580	5.93e-52	192
Msa0402430	MtrunA17_Chr1g0164591	30.671	313	175	4	113	423	1	273	8.48e-49	175
Msa0402430	MtrunA17_Chr1g0172971	83.673	98	16	0	1	98	20	117	1.42e-48	180
Msa0402430	MtrunA17_Chr1g0203721	23.708	658	353	17	22	632	68	623	2.82e-48	183
Msa0402430	MtrunA17_Chr7g0258861	31.378	341	183	7	12	349	2	294	2.31e-46	167
Msa0402430	MtrunA17_Chr2g0296781	24.684	632	376	18	23	632	3	556	8.42e-46	175

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0402430	AT4G38180.1	25.676	666	349	22	16	635	72	637	9.93e-48	181
Msa0402430	AT4G15090.1	23.296	631	373	18	32	635	66	612	2.62e-33	137
Msa0402430	AT4G15090.5	23.296	631	373	18	32	635	66	612	2.62e-33	137
Msa0402430	AT4G15090.3	23.296	631	373	18	32	635	66	612	2.62e-33	137
Msa0402430	AT4G15090.2	23.296	631	373	18	32	635	66	612	2.62e-33	137
Msa0402430	AT4G15090.4	23.296	631	373	18	32	635	66	612	2.62e-33	137
Msa0402430	AT5G28530.1	23.758	644	372	21	22	630	60	619	6.47e-32	132
Msa0402430	AT5G28530.3	23.950	643	368	20	22	630	60	615	1.15e-31	132
Msa0402430	AT5G28530.2	23.950	643	368	20	22	630	60	615	1.15e-31	132
Msa0402430	AT1G76320.3	23.364	642	342	19	32	632	12	544	1.92e-30	128

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Find 101 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACCTGGAAAACTATT+CGG	0.428349	3_2:+30585818	None:intergenic
AACAAACAAGGTTTCAAGAA+GGG	0.554561	3_2:-30587233	Msa0402430:CDS
AACAATATATATCGGCCATT+AGG	0.437083	3_2:-30586633	Msa0402430:intron
AACACAATAACGCACTAGCC+TGG	0.552106	3_2:+30585846	None:intergenic
AAGATCTTGGAGTTCCTAGG+TGG	0.662062	3_2:-30584993	Msa0402430:CDS
AATCGAACAAGTGAATGGTC+TGG	0.540971	3_2:+30585479	None:intergenic
ACAAAATAAGAAATCTGATA+AGG	0.474392	3_2:-30587279	Msa0402430:CDS
ACAGACGATATATACAGACC+AGG	0.696353	3_2:-30585864	Msa0402430:CDS
ACATTATGGTTCAGAGAATG+AGG	0.515275	3_2:-30585442	Msa0402430:CDS
AGAACGTCTAATGAAGTTAT+GGG	0.466325	3_2:-30586930	Msa0402430:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CGTTATGTTTGTAACAAACA+AGG	-	chr3_2:30584682-30584701	Msa0402430:CDS	30.0%
TAACAAACAAGGTTTCAAGA+AGG	-	chr3_2:30584693-30584712	Msa0402430:CDS	30.0%
AACAAACAAGGTTTCAAGAA+GGG	-	chr3_2:30584694-30584713	Msa0402430:CDS	30.0%
TTCACAAACTCAATAACCAA+TGG	+	chr3_2:30584760-30584779	None:intergenic	30.0%
TAAGATATATAGATGGGATG+TGG	-	chr3_2:30584858-30584877	Msa0402430:intron	30.0%
TTACTTCTCGAAATGATCTA+AGG	+	chr3_2:30584924-30584943	None:intergenic	30.0%
AGAACGTCTAATGAAGTTAT+GGG	-	chr3_2:30584997-30585016	Msa0402430:CDS	30.0%
TTTAAGCCTGAAAACCAAAA+AGG	+	chr3_2:30585051-30585070	None:intergenic	30.0%
AACAATATATATCGGCCATT+AGG	-	chr3_2:30585294-30585313	Msa0402430:CDS	30.0%
TAAGAACTATAGTCAAACCT+AGG	+	chr3_2:30585456-30585475	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	30584487	30587462	30584487	ID=Msa0402430;Name=Msa0402430
chr3_2	mRNA	30584487	30587462	30584487	ID=Msa0402430-mRNA-1;Parent=Msa0402430;Name=Msa0402430-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0\|0\|0\|0.8\|1\|1\|5\|0\|747
chr3_2	exon	30586634	30587462	30586634	ID=Msa0402430-mRNA-1:exon:6129;Parent=Msa0402430-mRNA-1
chr3_2	exon	30586340	30586432	30586340	ID=Msa0402430-mRNA-1:exon:6128;Parent=Msa0402430-mRNA-1
chr3_2	exon	30585625	30586016	30585625	ID=Msa0402430-mRNA-1:exon:6127;Parent=Msa0402430-mRNA-1
chr3_2	exon	30584887	30585501	30584887	ID=Msa0402430-mRNA-1:exon:6126;Parent=Msa0402430-mRNA-1
chr3_2	exon	30584487	30584801	30584487	ID=Msa0402430-mRNA-1:exon:6125;Parent=Msa0402430-mRNA-1
chr3_2	CDS	30586634	30587462	30586634	ID=Msa0402430-mRNA-1:cds;Parent=Msa0402430-mRNA-1
chr3_2	CDS	30586340	30586432	30586340	ID=Msa0402430-mRNA-1:cds;Parent=Msa0402430-mRNA-1
chr3_2	CDS	30585625	30586016	30585625	ID=Msa0402430-mRNA-1:cds;Parent=Msa0402430-mRNA-1
chr3_2	CDS	30584887	30585501	30584887	ID=Msa0402430-mRNA-1:cds;Parent=Msa0402430-mRNA-1
chr3_2	CDS	30584487	30584801	30584487	ID=Msa0402430-mRNA-1:cds;Parent=Msa0402430-mRNA-1

Gene Sequence

>Msa0402430

ATGATTCATGTTGATAGTTCTCAATGTTACTCCCCTAGCAATGAAGAATCATGGAAGTCTTTAATCTTTCATTCTATTAAAGAGATTGAAGATTTTTATGGTAACTATGCATATAACACTGGATTTTCTATTAGGACTATGTTGAAGTCGAAGAATAATTCACAAAATAAGAAATCTGATAAGGTACACTATGTGCGTTATGTTTGTAACAAACAAGGTTTCAAGAAGGGAAGTTTGCTTAATCCTAACAACAGGACAACATCAGACAGTCCATTGGTTATTGAGTTTGTGAAAGTGAAAGAAAAACCTGAGGAAAGGGTTGGTTGTAAAGCTGGAATATCTTTGAAGTTGGATGAAGTTTTGAATGTGTATAAGATATATAGATGGGATGTGGCTCACTGTCATCCATTACATAAACCAAATCATTCGTGTTACCTTAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTAGCTCTTATAAATTCTAAAGCTGGAATGTCGATGAGAACGTCTAATGAAGTTATGGGTCAAGGAGTTGGAGGCACGAAGAATCTTCCTTTTTGGTTTTCAGGCTTAAAGAACTATCTAATGACAATTCGTCAAAAAGAGATGGTGGTTGGTGAAGCAACTATAATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCATCTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCTAGTATTTTTTGGGTAGATGGAATTATGCAACTAGATTATTCTCTATTCGGCGATGTCATCAGTTTTGATACAACTTATCGAACAAACAATATATATCGGCCATTAGGGATTTTCTCGCTTCTGCCATTTGTTTTCATGGGGTTTTTGTCGATTCCAGATATCAAACCTGAAAGATGGTTTGAGACAAACTTAAATGATGCTGCCTTTATTGGTTTTGACAATCATCGTAAGAGTGTTTTATTTGGTGTTGCGCTATTGTACGATGAGATAACAACTACTTTTGATTGGTTGTTTAAAACATTCTTGAAGTGTATGTCCAATAAGAAACCACAGACGATATATACAGACCAGGCTAGTGCGTTATTGTGTTCAATTCCGAATAGTTTTCCAGGTGTTTTTCATGGGCTTTGTTCATGGCACATGGCAGAGAATGCAAAGAAAAATCTTGGCTCTCGTGCAAACAATGCATTTTTTGATGAGTTAACTAATTTGGTTTCAAATGTAGACGATGAATCAGATTTTGACTATAATTGGGATCAGATGATGAAAAATTGTTTTAATGGAAGGCCAACTTCAGACTTTAGCTTGCTTCCAGACCATTCACTTGTTCGATTCTTTAGTCATCTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGAGTTGGACTTCAAGGCTACAAACACTAGAACAAAAAATAATTATCCCAACAGGCAGCTGATGCGGTCCGTTGTAAACAAGTACACACCAGCTTGCTTTGCATTTATTCATAGGGAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACATAACAAAAGGGTCTGCCTTGGAGAAGTTTTTCAAAGTTTTCACAATTGAAAAGCTTGATGATAATTATGATGATGCTGATAATGACGGGCCTTCCACTGTTGATGTTTTGGATGCAGAACTTCAAGAAAACCTTTTCTCAAGTTTTGAAGATCATGATCGACTTGATGAAAGGGTTGTCGCAGTTGACATTAGAAGCAAAAGTTTTAGTTGTTCATGTCATATGTTTGAAAACAGGGGTTCCTTATGTCGACATGTTTTGAAGATCTTGGAGTTCCTAGGTGGTCATGTGCAATATAATTTTTTGAAGACAATACCTAGACAATATGTTATAAAACGTTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCGACCATCCATATCCGGAGGCATCTAAAATTTTTCTACACGGTTCTTGAAGCTGGGAAAAAAGCAGAGGAGTTACTTCTTTCTATAGGTATACGTACAGATCCATCATCTGTGACACCATCTAAGTCTTCAAATGTAGCTGCTGTTGGTGAAACATTTGCTGGGGTGAAGACCTCTGCACCAAAGTTCAAAGAAAGACCAAATCCAATTAAGTCAAAGAAGAGACTTAAAAGTGATTATGAAAAAGCTAGGGAAAGGCAGAAATTCCTTATAAATCGGATGAAACAAAAGAGAGATGATGAAGCTTTAAAAAAAAGTGGTCACTGA

Protein sequence

>Msa0402430

MIHVDSSQCYSPSNEESWKSLIFHSIKEIEDFYGNYAYNTGFSIRTMLKSKNNSQNKKSDKVHYVRYVCNKQGFKKGSLLNPNNRTTSDSPLVIEFVKVKEKPEERVGCKAGISLKLDEVLNVYKIYRWDVAHCHPLHKPNHSCYLRSFREVNEVQGQLALINSKAGMSMRTSNEVMGQGVGGTKNLPFWFSGLKNYLMTIRQKEMVVGEATIIQEFFRNEALSKPSFYYDIQVDAAEDIASIFWVDGIMQLDYSLFGDVISFDTTYRTNNIYRPLGIFSLLPFVFMGFLSIPDIKPERWFETNLNDAAFIGFDNHRKSVLFGVALLYDEITTTFDWLFKTFLKCMSNKKPQTIYTDQASALLCSIPNSFPGVFHGLCSWHMAENAKKNLGSRANNAFFDELTNLVSNVDDESDFDYNWDQMMKNCFNGRPTSDFSLLPDHSLVRFFSHLNIMVQRMRDNHAELDFKATNTRTKNNYPNRQLMRSVVNKYTPACFAFIHREYDLSFKYYYEEDITKGSALEKFFKVFTIEKLDDNYDDADNDGPSTVDVLDAELQENLFSSFEDHDRLDERVVAVDIRSKSFSCSCHMFENRGSLCRHVLKILEFLGGHVQYNFLKTIPRQYVIKRWTRDVRPSVDKLKSTIHIRRHLKFFYTVLEAGKKAEELLLSIGIRTDPSSVTPSKSSNVAAVGETFAGVKTSAPKFKERPNPIKSKKRLKSDYEKARERQKFLINRMKQKRDDEALKKSGH*