AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0402540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0402540 MtrunA17_Chr3g0092141 90.306 196 15 2 1 196 1 192 1.01e-128 360
Msa0402540 MtrunA17_Chr4g0060761 53.933 178 69 5 5 175 101 272 1.49e-54 174
Msa0402540 MtrunA17_Chr3g0127001 49.107 112 51 1 61 172 154 259 7.01e-31 113
Msa0402540 MtrunA17_Chr1g0182631 47.321 112 53 1 61 172 127 232 8.05e-29 107
Msa0402540 MtrunA17_Chr5g0406901 36.719 128 73 2 47 167 188 314 1.96e-22 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0402540 AT1G18790.1 62.500 128 41 2 48 168 112 239 8.17e-51 165
Msa0402540 AT1G74480.1 59.130 115 43 1 54 164 128 242 3.40e-43 146
Msa0402540 AT5G66990.1 50.746 134 59 2 42 170 142 273 8.21e-42 142
Msa0402540 AT5G53040.1 55.340 103 40 2 66 168 153 249 2.01e-31 115
Msa0402540 AT4G35590.2 37.313 134 72 2 61 187 192 320 1.99e-19 84.7
Msa0402540 AT4G35590.1 38.060 134 71 3 61 187 242 370 2.95e-19 84.7

Find 35 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTTGTACATTCTTTATA+AGG 0.172964 3_2:+30815117 None:intergenic
AATTATTCAATGACAGACTA+TGG 0.286299 3_2:-30815455 None:intergenic
AACTTGATGAAAACACAAAA+AGG 0.320323 3_2:-30814225 Msa0402540:CDS
TCTCTAGCAGCTTGAGATAT+TGG 0.323623 3_2:+30815230 None:intergenic
AAGAAAAGGTGTAGGGATCT+TGG 0.330015 3_2:-30815182 Msa0402540:CDS
GCTCATGGTTATGAGGTTAA+TGG 0.347444 3_2:-30814161 Msa0402540:CDS
TACTTGTACATTCTTTATAA+GGG 0.359802 3_2:+30815118 None:intergenic
ACTTGATGCTGGAAATTATT+CGG 0.365304 3_2:-30815393 Msa0402540:CDS
TCAATGACAGACTATGGTTA+TGG 0.408759 3_2:-30815449 Msa0402540:CDS
GGCTTACACATTTGAAGAAA+AGG 0.415724 3_2:-30815196 Msa0402540:CDS
CATTCTTTATAAGGGTTTGA+AGG 0.417292 3_2:+30815126 None:intergenic
TATGAGGTTAATGGAACAAC+AGG 0.443913 3_2:-30814152 Msa0402540:CDS
CTGCTAGAGACCTTAATGTT+GGG 0.489436 3_2:-30815217 Msa0402540:CDS
GAAAGAAATGAAGAAGGTAG+AGG 0.489935 3_2:-30814263 Msa0402540:CDS
ATGAATGTGATGAGAAGATA+AGG 0.493501 3_2:-30814306 Msa0402540:CDS
GCTTAGACAAGCATGTTTCA+AGG 0.494981 3_2:-30814203 Msa0402540:CDS
ATTGCAGAAAGAAATGAAGA+AGG 0.517898 3_2:-30814269 Msa0402540:CDS
AGAGAAGGCTCATGGTTATG+AGG 0.522137 3_2:-30814168 Msa0402540:CDS
GCTGCTAGAGACCTTAATGT+TGG 0.523622 3_2:-30815218 Msa0402540:CDS
AATGTGTAAGCCCAACATTA+AGG 0.530566 3_2:+30815207 None:intergenic
ATGTGGAATGAACTTGATGC+TGG 0.531093 3_2:-30815404 Msa0402540:CDS
CACATTTGAAGAAAAGGTGT+AGG 0.540159 3_2:-30815190 Msa0402540:CDS
AGGCTCATTAATTTCCTATG+TGG 0.543250 3_2:+30815146 None:intergenic
GAATTCAAAGGTGGCCACAT+AGG 0.544869 3_2:-30815160 Msa0402540:CDS
TACTGGAGAAAATGTTGCAA+AGG 0.556786 3_2:-30815342 Msa0402540:CDS
AGGCTAATTACAAGAAGAGA+AGG 0.568624 3_2:-30814183 Msa0402540:CDS
TGTTGCAAAGGAAATTGTGA+AGG 0.572064 3_2:-30815330 Msa0402540:CDS
ACATTTGAAGAAAAGGTGTA+GGG 0.575896 3_2:-30815189 Msa0402540:CDS
TTATTGGTGGTGCAGGAGCA+AGG 0.586089 3_2:-30814331 Msa0402540:intron
TTACAAGAAGAGAAGGCTCA+TGG 0.590126 3_2:-30814176 Msa0402540:CDS
GTAGGGATCTTGGAATTCAA+AGG 0.603743 3_2:-30815172 Msa0402540:CDS
GTTGCAAAGGAAATTGTGAA+GGG 0.628143 3_2:-30815329 Msa0402540:CDS
GCATCAAGTTCATTCCACAT+TGG 0.632758 3_2:+30815407 None:intergenic
TGAGAAGATAAGGAATGCTG+TGG 0.656309 3_2:-30814296 Msa0402540:CDS
GGGATCTTGGAATTCAAAGG+TGG 0.676630 3_2:-30815169 Msa0402540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATAACTCAAAAAATAT+GGG + chr3_2:30814439-30814458 None:intergenic 10.0%
!! TATTAATAACTCAAAAAATA+TGG + chr3_2:30814440-30814459 None:intergenic 10.0%
!!! ATATATTTTAGAAAAATATG+AGG - chr3_2:30814878-30814897 Msa0402540:intron 10.0%
!!! TTTTATTTATTAATTATTGG+TGG - chr3_2:30815183-30815202 Msa0402540:CDS 10.0%
!! ATTAATAACTCAAAAAATAT+GGG + chr3_2:30814439-30814458 None:intergenic 10.0%
!! TATTAATAACTCAAAAAATA+TGG + chr3_2:30814440-30814459 None:intergenic 10.0%
!!! ATATATTTTAGAAAAATATG+AGG - chr3_2:30814878-30814897 Msa0402540:intron 10.0%
!!! TTTTATTTATTAATTATTGG+TGG - chr3_2:30815183-30815202 Msa0402540:CDS 10.0%
!!! AAAAAGAAAATCATGATTTT+AGG + chr3_2:30814984-30815003 None:intergenic 15.0%
!!! AAAAAGAAAATCATGATTTT+AGG + chr3_2:30814984-30815003 None:intergenic 15.0%
!! TACTTGTACATTCTTTATAA+GGG + chr3_2:30814412-30814431 None:intergenic 20.0%
!! TTACTTGTACATTCTTTATA+AGG + chr3_2:30814413-30814432 None:intergenic 20.0%
!! TGTTAAAAGTTATAGCAAAA+AGG - chr3_2:30814840-30814859 Msa0402540:intron 20.0%
!!! TAGGTTAATTTTCACTATAA+GGG + chr3_2:30814904-30814923 None:intergenic 20.0%
!!! TTAGGTTAATTTTCACTATA+AGG + chr3_2:30814905-30814924 None:intergenic 20.0%
!!! GGAATTGAAAAATCAATTTT+AGG + chr3_2:30814923-30814942 None:intergenic 20.0%
!!! CTTTTTCTTACTAATTTTTG+TGG - chr3_2:30814998-30815017 Msa0402540:intron 20.0%
!! TACTTGTACATTCTTTATAA+GGG + chr3_2:30814412-30814431 None:intergenic 20.0%
!! TTACTTGTACATTCTTTATA+AGG + chr3_2:30814413-30814432 None:intergenic 20.0%
!! TGTTAAAAGTTATAGCAAAA+AGG - chr3_2:30814840-30814859 Msa0402540:intron 20.0%
!!! TAGGTTAATTTTCACTATAA+GGG + chr3_2:30814904-30814923 None:intergenic 20.0%
!!! TTAGGTTAATTTTCACTATA+AGG + chr3_2:30814905-30814924 None:intergenic 20.0%
!!! GGAATTGAAAAATCAATTTT+AGG + chr3_2:30814923-30814942 None:intergenic 20.0%
!!! CTTTTTCTTACTAATTTTTG+TGG - chr3_2:30814998-30815017 Msa0402540:intron 20.0%
!!! TGTAGTGAATTGTTTTAATG+TGG - chr3_2:30815044-30815063 Msa0402540:intron 25.0%
! AACTTGATGAAAACACAAAA+AGG - chr3_2:30815302-30815321 Msa0402540:CDS 25.0%
!!! TGTAGTGAATTGTTTTAATG+TGG - chr3_2:30815044-30815063 Msa0402540:intron 25.0%
! AACTTGATGAAAACACAAAA+AGG - chr3_2:30815302-30815321 Msa0402540:CDS 25.0%
! TTATGGTGATGCTAAAAATG+TGG - chr3_2:30814095-30814114 Msa0402540:CDS 30.0%
ACTTGATGCTGGAAATTATT+CGG - chr3_2:30814134-30814153 Msa0402540:CDS 30.0%
! TTTTCGATTAGCAACATTAC+TGG - chr3_2:30814168-30814187 Msa0402540:CDS 30.0%
! ACATTTGAAGAAAAGGTGTA+GGG - chr3_2:30814338-30814357 Msa0402540:intron 30.0%
CATTCTTTATAAGGGTTTGA+AGG + chr3_2:30814404-30814423 None:intergenic 30.0%
!!! CACAATGTGATTTTTTGGTT+TGG + chr3_2:30814528-30814547 None:intergenic 30.0%
!!! CACTACACAATGTGATTTTT+TGG + chr3_2:30814533-30814552 None:intergenic 30.0%
AAAAATCACATTGTGTAGTG+AGG - chr3_2:30814533-30814552 Msa0402540:intron 30.0%
ATGTTTGCGCAAGTAAATTA+TGG + chr3_2:30815094-30815113 None:intergenic 30.0%
!! TTATTAATTATTGGTGGTGC+AGG - chr3_2:30815189-30815208 Msa0402540:CDS 30.0%
ATGAATGTGATGAGAAGATA+AGG - chr3_2:30815221-30815240 Msa0402540:CDS 30.0%
ATTGCAGAAAGAAATGAAGA+AGG - chr3_2:30815258-30815277 Msa0402540:CDS 30.0%
!! TTTTCATCAAGTTGCAAATC+TGG + chr3_2:30815295-30815314 None:intergenic 30.0%
! TTATGGTGATGCTAAAAATG+TGG - chr3_2:30814095-30814114 Msa0402540:CDS 30.0%
ACTTGATGCTGGAAATTATT+CGG - chr3_2:30814134-30814153 Msa0402540:CDS 30.0%
! TTTTCGATTAGCAACATTAC+TGG - chr3_2:30814168-30814187 Msa0402540:CDS 30.0%
! ACATTTGAAGAAAAGGTGTA+GGG - chr3_2:30814338-30814357 Msa0402540:intron 30.0%
CATTCTTTATAAGGGTTTGA+AGG + chr3_2:30814404-30814423 None:intergenic 30.0%
!!! CACAATGTGATTTTTTGGTT+TGG + chr3_2:30814528-30814547 None:intergenic 30.0%
!!! CACTACACAATGTGATTTTT+TGG + chr3_2:30814533-30814552 None:intergenic 30.0%
AAAAATCACATTGTGTAGTG+AGG - chr3_2:30814533-30814552 Msa0402540:intron 30.0%
ATGTTTGCGCAAGTAAATTA+TGG + chr3_2:30815094-30815113 None:intergenic 30.0%
!! TTATTAATTATTGGTGGTGC+AGG - chr3_2:30815189-30815208 Msa0402540:CDS 30.0%
ATGAATGTGATGAGAAGATA+AGG - chr3_2:30815221-30815240 Msa0402540:CDS 30.0%
ATTGCAGAAAGAAATGAAGA+AGG - chr3_2:30815258-30815277 Msa0402540:CDS 30.0%
!! TTTTCATCAAGTTGCAAATC+TGG + chr3_2:30815295-30815314 None:intergenic 30.0%
CTAAAAATGTGGTTCCAATG+TGG - chr3_2:30814106-30814125 Msa0402540:CDS 35.0%
TACTGGAGAAAATGTTGCAA+AGG - chr3_2:30814185-30814204 Msa0402540:CDS 35.0%
TGTTGCAAAGGAAATTGTGA+AGG - chr3_2:30814197-30814216 Msa0402540:CDS 35.0%
GTTGCAAAGGAAATTGTGAA+GGG - chr3_2:30814198-30814217 Msa0402540:CDS 35.0%
AATGTGTAAGCCCAACATTA+AGG + chr3_2:30814323-30814342 None:intergenic 35.0%
GGCTTACACATTTGAAGAAA+AGG - chr3_2:30814331-30814350 Msa0402540:intron 35.0%
! CACATTTGAAGAAAAGGTGT+AGG - chr3_2:30814337-30814356 Msa0402540:intron 35.0%
AGGCTCATTAATTTCCTATG+TGG + chr3_2:30814384-30814403 None:intergenic 35.0%
CAAGTCGCAAAATTTAGAGA+TGG + chr3_2:30814944-30814963 None:intergenic 35.0%
! CATCTCTAAATTTTGCGACT+TGG - chr3_2:30814942-30814961 Msa0402540:intron 35.0%
!!! AAATTTTGCGACTTGGTAAG+CGG - chr3_2:30814949-30814968 Msa0402540:intron 35.0%
GAAAGAAATGAAGAAGGTAG+AGG - chr3_2:30815264-30815283 Msa0402540:CDS 35.0%
AGGCTAATTACAAGAAGAGA+AGG - chr3_2:30815344-30815363 Msa0402540:CDS 35.0%
TATGAGGTTAATGGAACAAC+AGG - chr3_2:30815375-30815394 Msa0402540:CDS 35.0%
CTAAAAATGTGGTTCCAATG+TGG - chr3_2:30814106-30814125 Msa0402540:CDS 35.0%
TACTGGAGAAAATGTTGCAA+AGG - chr3_2:30814185-30814204 Msa0402540:CDS 35.0%
TGTTGCAAAGGAAATTGTGA+AGG - chr3_2:30814197-30814216 Msa0402540:CDS 35.0%
GTTGCAAAGGAAATTGTGAA+GGG - chr3_2:30814198-30814217 Msa0402540:CDS 35.0%
AATGTGTAAGCCCAACATTA+AGG + chr3_2:30814323-30814342 None:intergenic 35.0%
GGCTTACACATTTGAAGAAA+AGG - chr3_2:30814331-30814350 Msa0402540:intron 35.0%
! CACATTTGAAGAAAAGGTGT+AGG - chr3_2:30814337-30814356 Msa0402540:intron 35.0%
AGGCTCATTAATTTCCTATG+TGG + chr3_2:30814384-30814403 None:intergenic 35.0%
CAAGTCGCAAAATTTAGAGA+TGG + chr3_2:30814944-30814963 None:intergenic 35.0%
! CATCTCTAAATTTTGCGACT+TGG - chr3_2:30814942-30814961 Msa0402540:intron 35.0%
!!! AAATTTTGCGACTTGGTAAG+CGG - chr3_2:30814949-30814968 Msa0402540:intron 35.0%
GAAAGAAATGAAGAAGGTAG+AGG - chr3_2:30815264-30815283 Msa0402540:CDS 35.0%
AGGCTAATTACAAGAAGAGA+AGG - chr3_2:30815344-30815363 Msa0402540:CDS 35.0%
TATGAGGTTAATGGAACAAC+AGG - chr3_2:30815375-30815394 Msa0402540:CDS 35.0%
GCATCAAGTTCATTCCACAT+TGG + chr3_2:30814123-30814142 None:intergenic 40.0%
ATGTGGAATGAACTTGATGC+TGG - chr3_2:30814123-30814142 Msa0402540:CDS 40.0%
TCTCTAGCAGCTTGAGATAT+TGG + chr3_2:30814300-30814319 None:intergenic 40.0%
CTGCTAGAGACCTTAATGTT+GGG - chr3_2:30814310-30814329 Msa0402540:CDS 40.0%
! AAGAAAAGGTGTAGGGATCT+TGG - chr3_2:30814345-30814364 Msa0402540:intron 40.0%
GTAGGGATCTTGGAATTCAA+AGG - chr3_2:30814355-30814374 Msa0402540:intron 40.0%
ATTGTGTAGTGAGGAGATCT+AGG - chr3_2:30814542-30814561 Msa0402540:intron 40.0%
TTGTGTAGTGAGGAGATCTA+GGG - chr3_2:30814543-30814562 Msa0402540:intron 40.0%
TGAGAAGATAAGGAATGCTG+TGG - chr3_2:30815231-30815250 Msa0402540:CDS 40.0%
GCTTAGACAAGCATGTTTCA+AGG - chr3_2:30815324-30815343 Msa0402540:CDS 40.0%
TTACAAGAAGAGAAGGCTCA+TGG - chr3_2:30815351-30815370 Msa0402540:CDS 40.0%
GCTCATGGTTATGAGGTTAA+TGG - chr3_2:30815366-30815385 Msa0402540:CDS 40.0%
GCATCAAGTTCATTCCACAT+TGG + chr3_2:30814123-30814142 None:intergenic 40.0%
ATGTGGAATGAACTTGATGC+TGG - chr3_2:30814123-30814142 Msa0402540:CDS 40.0%
TCTCTAGCAGCTTGAGATAT+TGG + chr3_2:30814300-30814319 None:intergenic 40.0%
CTGCTAGAGACCTTAATGTT+GGG - chr3_2:30814310-30814329 Msa0402540:CDS 40.0%
! AAGAAAAGGTGTAGGGATCT+TGG - chr3_2:30814345-30814364 Msa0402540:intron 40.0%
GTAGGGATCTTGGAATTCAA+AGG - chr3_2:30814355-30814374 Msa0402540:intron 40.0%
ATTGTGTAGTGAGGAGATCT+AGG - chr3_2:30814542-30814561 Msa0402540:intron 40.0%
TTGTGTAGTGAGGAGATCTA+GGG - chr3_2:30814543-30814562 Msa0402540:intron 40.0%
TGAGAAGATAAGGAATGCTG+TGG - chr3_2:30815231-30815250 Msa0402540:CDS 40.0%
GCTTAGACAAGCATGTTTCA+AGG - chr3_2:30815324-30815343 Msa0402540:CDS 40.0%
TTACAAGAAGAGAAGGCTCA+TGG - chr3_2:30815351-30815370 Msa0402540:CDS 40.0%
GCTCATGGTTATGAGGTTAA+TGG - chr3_2:30815366-30815385 Msa0402540:CDS 40.0%
GCTGCTAGAGACCTTAATGT+TGG - chr3_2:30814309-30814328 Msa0402540:CDS 45.0%
GGGATCTTGGAATTCAAAGG+TGG - chr3_2:30814358-30814377 Msa0402540:intron 45.0%
GAATTCAAAGGTGGCCACAT+AGG - chr3_2:30814367-30814386 Msa0402540:intron 45.0%
AGAGAAGGCTCATGGTTATG+AGG - chr3_2:30815359-30815378 Msa0402540:CDS 45.0%
GCTGCTAGAGACCTTAATGT+TGG - chr3_2:30814309-30814328 Msa0402540:CDS 45.0%
GGGATCTTGGAATTCAAAGG+TGG - chr3_2:30814358-30814377 Msa0402540:intron 45.0%
GAATTCAAAGGTGGCCACAT+AGG - chr3_2:30814367-30814386 Msa0402540:intron 45.0%
AGAGAAGGCTCATGGTTATG+AGG - chr3_2:30815359-30815378 Msa0402540:CDS 45.0%
!!! ACATTTTATTTATTAATTAT+TGG - chr3_2:30815180-30815199 Msa0402540:CDS 5.0%
!!! ACATTTTATTTATTAATTAT+TGG - chr3_2:30815180-30815199 Msa0402540:CDS 5.0%
!! TTATTGGTGGTGCAGGAGCA+AGG - chr3_2:30815196-30815215 Msa0402540:CDS 50.0%
!! TTATTGGTGGTGCAGGAGCA+AGG - chr3_2:30815196-30815215 Msa0402540:CDS 50.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 30814081 30815468 30814081 ID=Msa0402540;Name=Msa0402540
chr3_2 mRNA 30814081 30815468 30814081 ID=Msa0402540-mRNA-1;Parent=Msa0402540;Name=Msa0402540-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|201
chr3_2 exon 30815121 30815468 30815121 ID=Msa0402540-mRNA-1:exon:6164;Parent=Msa0402540-mRNA-1
chr3_2 exon 30814081 30814338 30814081 ID=Msa0402540-mRNA-1:exon:6163;Parent=Msa0402540-mRNA-1
chr3_2 CDS 30815121 30815468 30815121 ID=Msa0402540-mRNA-1:cds;Parent=Msa0402540-mRNA-1
chr3_2 CDS 30814081 30814338 30814081 ID=Msa0402540-mRNA-1:cds;Parent=Msa0402540-mRNA-1
Gene Sequence

>Msa0402540

ATGACAGACTATGGTTATGGTGATGCTAAAAATGTGGTTCCAATGTGGAATGAACTTGATGCTGGAAATTATTCGGATAAACATATCTTTTCGATTAGCAACATTACTGGAGAAAATGTTGCAAAGGAAATTGTGAAGGGTAAGAGATGTAGAGAAGAGAATACTAATAGTGCAAGAATGCTAACAAAAGAAACAATTTCTGAGTACTTCTATGTGCCAATATCTCAAGCTGCTAGAGACCTTAATGTTGGGCTTACACATTTGAAGAAAAGGTGTAGGGATCTTGGAATTCAAAGGTGGCCACATAGGAAATTAATGAGCCTTCAAACCCTTATAAAGAATGTACAAGAGCAAGGTAATGAATGTGATGAGAAGATAAGGAATGCTGTGGAAGTATTGCAGAAAGAAATGAAGAAGGTAGAGGAAAAGCCAGATTTGCAACTTGATGAAAACACAAAAAGGCTTAGACAAGCATGTTTCAAGGCTAATTACAAGAAGAGAAGGCTCATGGTTATGAGGTTAATGGAACAACAGGAGTCTTCTCTCAGTGAAAGTGCAAGTGTTGATGATCACAAAAAGTCAAATTTCTTCTTAACATCATGTTAA

Protein sequence

>Msa0402540

MTDYGYGDAKNVVPMWNELDAGNYSDKHIFSISNITGENVAKEIVKGKRCREENTNSARMLTKETISEYFYVPISQAARDLNVGLTHLKKRCRDLGIQRWPHRKLMSLQTLIKNVQEQGNECDEKIRNAVEVLQKEMKKVEEKPDLQLDENTKRLRQACFKANYKKRRLMVMRLMEQQESSLSESASVDDHKKSNFFLTSC*