Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0406440 | AES73232.2 | 93.023 | 43 | 3 | 0 | 34 | 76 | 1288 | 1330 | 4.91e-17 | 86.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0406440 | G7J5R6 | 93.023 | 43 | 3 | 0 | 34 | 76 | 1288 | 1330 | 2.35e-17 | 86.3 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0406440 | TR | TAZ |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0406440 | MtrunA17_Chr3g0133461 | 93.023 | 43 | 3 | 0 | 34 | 76 | 1236 | 1278 | 5.17e-21 | 85.9 |
| Msa0406440 | MtrunA17_Chr3g0133461 | 86.486 | 37 | 5 | 0 | 1 | 37 | 1076 | 1112 | 7.78e-17 | 74.3 |
| Msa0406440 | MtrunA17_Chr5g0438991 | 76.000 | 50 | 12 | 0 | 27 | 76 | 860 | 909 | 7.25e-20 | 82.8 |
| Msa0406440 | MtrunA17_Chr5g0438991 | 70.270 | 37 | 11 | 0 | 1 | 37 | 760 | 796 | 6.45e-12 | 60.1 |
| Msa0406440 | MtrunA17_Chr3g0138411 | 71.667 | 60 | 12 | 2 | 17 | 76 | 1087 | 1141 | 1.38e-19 | 82.0 |
| Msa0406440 | MtrunA17_Chr3g0138411 | 78.378 | 37 | 8 | 0 | 1 | 37 | 947 | 983 | 6.91e-14 | 65.9 |
| Msa0406440 | MtrunA17_Chr6g0456861 | 71.429 | 49 | 12 | 1 | 34 | 80 | 670 | 718 | 1.80e-16 | 73.2 |
| Msa0406440 | MtrunA17_Chr5g0401991 | 75.676 | 37 | 9 | 0 | 1 | 37 | 86 | 122 | 1.21e-13 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 122 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAACTCGCCATTCTTTGA+TGG | 0.393855 | 3_2:-43148560 | None:intergenic |
| CACTAGAATTTCGCACCTAC+TGG | 0.421340 | 3_2:-43149290 | None:intergenic |
| ATCATTATGTTTAGTGTTGG+AGG | 0.449050 | 3_2:-43148701 | None:intergenic |
| CGAGTTGCATACTCTTTGTC+AGG | 0.461409 | 3_2:+43148574 | Msa0406440:CDS |
| TTCTTGATTTGAGAACAGTT+TGG | 0.474837 | 3_2:-43149253 | None:intergenic |
| AATATCATTATGTTTAGTGT+TGG | 0.474871 | 3_2:-43148704 | None:intergenic |
| ATGGGTGTGCATCAGAACTA+TGG | 0.476458 | 3_2:-43148541 | None:intergenic |
| CATTATGTTTAGTGTTGGAG+GGG | 0.505283 | 3_2:-43148699 | None:intergenic |
| GCAACTCGCCATTCTTTGAT+GGG | 0.505596 | 3_2:-43148559 | None:intergenic |
| TCATTATGTTTAGTGTTGGA+GGG | 0.517970 | 3_2:-43148700 | None:intergenic |
| GAAAGTGACAAAGCAGTCGT+TGG | 0.555810 | 3_2:+43149604 | Msa0406440:CDS |
| GATGCACACCCATCAAAGAA+TGG | 0.560620 | 3_2:+43148551 | Msa0406440:CDS |
| AGCTGTTCTCTCATGCCAGT+AGG | 0.676221 | 3_2:+43149275 | Msa0406440:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATCATTATGTTTAGTGT+TGG | - | chr3_2:43148707-43148726 | None:intergenic | 20.0% |
| !! | AGAGTAGATTATTTGAAAAT+AGG | + | chr3_2:43148734-43148753 | Msa0406440:intron | 20.0% |
| !! | GAGTAGATTATTTGAAAATA+GGG | + | chr3_2:43148735-43148754 | Msa0406440:intron | 20.0% |
| !! | GTATTGAAAACAACAAATAT+AGG | - | chr3_2:43149111-43149130 | None:intergenic | 20.0% |
| !! | TTTAACCATAAAAAATGACT+TGG | - | chr3_2:43149496-43149515 | None:intergenic | 20.0% |
| !!! | TATATTTATGTTGATTTTGC+AGG | + | chr3_2:43149540-43149559 | Msa0406440:intron | 20.0% |
| !!! | ATATTTATGTTGATTTTGCA+GGG | + | chr3_2:43149541-43149560 | Msa0406440:intron | 20.0% |
| !! | AATATCATTATGTTTAGTGT+TGG | - | chr3_2:43148707-43148726 | None:intergenic | 20.0% |
| !! | AGAGTAGATTATTTGAAAAT+AGG | + | chr3_2:43148734-43148753 | Msa0406440:intron | 20.0% |
| !! | GAGTAGATTATTTGAAAATA+GGG | + | chr3_2:43148735-43148754 | Msa0406440:intron | 20.0% |
| !! | GTATTGAAAACAACAAATAT+AGG | - | chr3_2:43149111-43149130 | None:intergenic | 20.0% |
| !! | TTTAACCATAAAAAATGACT+TGG | - | chr3_2:43149496-43149515 | None:intergenic | 20.0% |
| !!! | TATATTTATGTTGATTTTGC+AGG | + | chr3_2:43149540-43149559 | Msa0406440:intron | 20.0% |
| !!! | ATATTTATGTTGATTTTGCA+GGG | + | chr3_2:43149541-43149560 | Msa0406440:intron | 20.0% |
| ! | ATATGAAAAACATCAGGTTT+AGG | - | chr3_2:43148606-43148625 | None:intergenic | 25.0% |
| ! | AGAATCATATGAAAAACATC+AGG | - | chr3_2:43148612-43148631 | None:intergenic | 25.0% |
| ! | TTTCAAATAATCTACTCTCA+AGG | - | chr3_2:43148732-43148751 | None:intergenic | 25.0% |
| ! | TGATTACCAGATTGATATAT+TGG | - | chr3_2:43149055-43149074 | None:intergenic | 25.0% |
| ! | AATATATCAATCTGGTAATC+AGG | + | chr3_2:43149054-43149073 | Msa0406440:intron | 25.0% |
| !!! | TTACTTTCTGACTTGTTTTT+TGG | + | chr3_2:43149465-43149484 | Msa0406440:intron | 25.0% |
| !!! | TACTTTCTGACTTGTTTTTT+GGG | + | chr3_2:43149466-43149485 | Msa0406440:intron | 25.0% |
| ! | ATATGAAAAACATCAGGTTT+AGG | - | chr3_2:43148606-43148625 | None:intergenic | 25.0% |
| ! | AGAATCATATGAAAAACATC+AGG | - | chr3_2:43148612-43148631 | None:intergenic | 25.0% |
| ! | TTTCAAATAATCTACTCTCA+AGG | - | chr3_2:43148732-43148751 | None:intergenic | 25.0% |
| ! | TGATTACCAGATTGATATAT+TGG | - | chr3_2:43149055-43149074 | None:intergenic | 25.0% |
| ! | AATATATCAATCTGGTAATC+AGG | + | chr3_2:43149054-43149073 | Msa0406440:intron | 25.0% |
| !!! | TTACTTTCTGACTTGTTTTT+TGG | + | chr3_2:43149465-43149484 | Msa0406440:intron | 25.0% |
| !!! | TACTTTCTGACTTGTTTTTT+GGG | + | chr3_2:43149466-43149485 | Msa0406440:intron | 25.0% |
| ! | TTGAATTGTCTTCCATTTTC+AGG | + | chr3_2:43148660-43148679 | Msa0406440:intron | 30.0% |
| ! | TCATTATGTTTAGTGTTGGA+GGG | - | chr3_2:43148703-43148722 | None:intergenic | 30.0% |
| ! | ATCATTATGTTTAGTGTTGG+AGG | - | chr3_2:43148704-43148723 | None:intergenic | 30.0% |
| TTTGATTCAACTTGTGTTCA+TGG | - | chr3_2:43148979-43148998 | None:intergenic | 30.0% | |
| TCAAGACCAATATATCAATC+TGG | + | chr3_2:43149046-43149065 | Msa0406440:intron | 30.0% | |
| ATCAGGAGTAAAATGAGAAA+AGG | + | chr3_2:43149071-43149090 | Msa0406440:intron | 30.0% | |
| TCAGGAGTAAAATGAGAAAA+GGG | + | chr3_2:43149072-43149091 | Msa0406440:intron | 30.0% | |
| ! | TTCTTGATTTGAGAACAGTT+TGG | - | chr3_2:43149256-43149275 | None:intergenic | 30.0% |
| CAAAATCTCTCAAATTCTCT+AGG | - | chr3_2:43149438-43149457 | None:intergenic | 30.0% | |
| ! | CTAGAGAATTTGAGAGATTT+TGG | + | chr3_2:43149436-43149455 | Msa0406440:intron | 30.0% |
| !!! | GACAACCAAGTCATTTTTTA+TGG | + | chr3_2:43149488-43149507 | Msa0406440:intron | 30.0% |
| ! | TTGAATTGTCTTCCATTTTC+AGG | + | chr3_2:43148660-43148679 | Msa0406440:intron | 30.0% |
| ! | TCATTATGTTTAGTGTTGGA+GGG | - | chr3_2:43148703-43148722 | None:intergenic | 30.0% |
| ! | ATCATTATGTTTAGTGTTGG+AGG | - | chr3_2:43148704-43148723 | None:intergenic | 30.0% |
| TTTGATTCAACTTGTGTTCA+TGG | - | chr3_2:43148979-43148998 | None:intergenic | 30.0% | |
| TCAAGACCAATATATCAATC+TGG | + | chr3_2:43149046-43149065 | Msa0406440:intron | 30.0% | |
| ATCAGGAGTAAAATGAGAAA+AGG | + | chr3_2:43149071-43149090 | Msa0406440:intron | 30.0% | |
| TCAGGAGTAAAATGAGAAAA+GGG | + | chr3_2:43149072-43149091 | Msa0406440:intron | 30.0% | |
| ! | TTCTTGATTTGAGAACAGTT+TGG | - | chr3_2:43149256-43149275 | None:intergenic | 30.0% |
| CAAAATCTCTCAAATTCTCT+AGG | - | chr3_2:43149438-43149457 | None:intergenic | 30.0% | |
| ! | CTAGAGAATTTGAGAGATTT+TGG | + | chr3_2:43149436-43149455 | Msa0406440:intron | 30.0% |
| !!! | GACAACCAAGTCATTTTTTA+TGG | + | chr3_2:43149488-43149507 | Msa0406440:intron | 30.0% |
| ! | CATTATGTTTAGTGTTGGAG+GGG | - | chr3_2:43148702-43148721 | None:intergenic | 35.0% |
| TTTACAACAGACATCAGAGT+AGG | - | chr3_2:43148859-43148878 | None:intergenic | 35.0% | |
| CTTATAGCATGCAAAGCAAA+TGG | - | chr3_2:43148939-43148958 | None:intergenic | 35.0% | |
| CATTTGCTTTGCATGCTATA+AGG | + | chr3_2:43148937-43148956 | Msa0406440:intron | 35.0% | |
| TTTGCATGCTATAAGGATAG+AGG | + | chr3_2:43148944-43148963 | Msa0406440:intron | 35.0% | |
| ! | ACCATATCTTTTTACACTGC+TGG | - | chr3_2:43149327-43149346 | None:intergenic | 35.0% |
| ! | CATTATGTTTAGTGTTGGAG+GGG | - | chr3_2:43148702-43148721 | None:intergenic | 35.0% |
| TTTACAACAGACATCAGAGT+AGG | - | chr3_2:43148859-43148878 | None:intergenic | 35.0% | |
| CTTATAGCATGCAAAGCAAA+TGG | - | chr3_2:43148939-43148958 | None:intergenic | 35.0% | |
| CATTTGCTTTGCATGCTATA+AGG | + | chr3_2:43148937-43148956 | Msa0406440:intron | 35.0% | |
| TTTGCATGCTATAAGGATAG+AGG | + | chr3_2:43148944-43148963 | Msa0406440:intron | 35.0% | |
| ! | ACCATATCTTTTTACACTGC+TGG | - | chr3_2:43149327-43149346 | None:intergenic | 35.0% |
| CATCAAAAACGGCCTGAAAA+TGG | - | chr3_2:43148675-43148694 | None:intergenic | 40.0% | |
| GGAGGGGATATCATCAAAAA+CGG | - | chr3_2:43148686-43148705 | None:intergenic | 40.0% | |
| CATCTGTATTCCAAAAGAGC+TGG | + | chr3_2:43148888-43148907 | Msa0406440:intron | 40.0% | |
| ! | TCACACATTTCCAGCTCTTT+TGG | - | chr3_2:43148901-43148920 | None:intergenic | 40.0% |
| TTCACCATCTGTGTGTAAGT+CGG | - | chr3_2:43149169-43149188 | None:intergenic | 40.0% | |
| GGTGCGAAATTCTAGTGAAT+CGG | + | chr3_2:43149296-43149315 | Msa0406440:intron | 40.0% | |
| GTGCGAAATTCTAGTGAATC+GGG | + | chr3_2:43149297-43149316 | Msa0406440:intron | 40.0% | |
| TGCGAAATTCTAGTGAATCG+GGG | + | chr3_2:43149298-43149317 | Msa0406440:intron | 40.0% | |
| GCCAGCAGTGTAAAAAGATA+TGG | + | chr3_2:43149323-43149342 | Msa0406440:intron | 40.0% | |
| ! | TTATACTTGCTGCTCACTCT+AGG | + | chr3_2:43149347-43149366 | Msa0406440:intron | 40.0% |
| ! | CTCTAGGAATTGCAAAGACT+AGG | + | chr3_2:43149363-43149382 | Msa0406440:intron | 40.0% |
| CATCAAAAACGGCCTGAAAA+TGG | - | chr3_2:43148675-43148694 | None:intergenic | 40.0% | |
| GGAGGGGATATCATCAAAAA+CGG | - | chr3_2:43148686-43148705 | None:intergenic | 40.0% | |
| CATCTGTATTCCAAAAGAGC+TGG | + | chr3_2:43148888-43148907 | Msa0406440:intron | 40.0% | |
| ! | TCACACATTTCCAGCTCTTT+TGG | - | chr3_2:43148901-43148920 | None:intergenic | 40.0% |
| TTCACCATCTGTGTGTAAGT+CGG | - | chr3_2:43149169-43149188 | None:intergenic | 40.0% | |
| GGTGCGAAATTCTAGTGAAT+CGG | + | chr3_2:43149296-43149315 | Msa0406440:intron | 40.0% | |
| GTGCGAAATTCTAGTGAATC+GGG | + | chr3_2:43149297-43149316 | Msa0406440:intron | 40.0% | |
| TGCGAAATTCTAGTGAATCG+GGG | + | chr3_2:43149298-43149317 | Msa0406440:intron | 40.0% | |
| GCCAGCAGTGTAAAAAGATA+TGG | + | chr3_2:43149323-43149342 | Msa0406440:intron | 40.0% | |
| ! | TTATACTTGCTGCTCACTCT+AGG | + | chr3_2:43149347-43149366 | Msa0406440:intron | 40.0% |
| ! | CTCTAGGAATTGCAAAGACT+AGG | + | chr3_2:43149363-43149382 | Msa0406440:intron | 40.0% |
| ATGGGTGTGCATCAGAACTA+TGG | - | chr3_2:43148544-43148563 | None:intergenic | 45.0% | |
| GATGCACACCCATCAAAGAA+TGG | + | chr3_2:43148551-43148570 | Msa0406440:CDS | 45.0% | |
| GCAACTCGCCATTCTTTGAT+GGG | - | chr3_2:43148562-43148581 | None:intergenic | 45.0% | |
| TGCAACTCGCCATTCTTTGA+TGG | - | chr3_2:43148563-43148582 | None:intergenic | 45.0% | |
| CGAGTTGCATACTCTTTGTC+AGG | + | chr3_2:43148574-43148593 | Msa0406440:CDS | 45.0% | |
| TGCCAAGTATTCCTCGAAGA+TGG | + | chr3_2:43148813-43148832 | Msa0406440:intron | 45.0% | |
| GACCATCTTCGAGGAATACT+TGG | - | chr3_2:43148818-43148837 | None:intergenic | 45.0% | |
| TAGGATTGCGTAGATGGTAG+AGG | - | chr3_2:43148840-43148859 | None:intergenic | 45.0% | |
| TCAGAGTAGGATTGCGTAGA+TGG | - | chr3_2:43148846-43148865 | None:intergenic | 45.0% | |
| GCAAAGCAAATGGTGCACTA+TGG | - | chr3_2:43148929-43148948 | None:intergenic | 45.0% | |
| CACTAGAATTTCGCACCTAC+TGG | - | chr3_2:43149293-43149312 | None:intergenic | 45.0% | |
| ! | ACTAGGAGTGTAGCATTCCA+CGG | + | chr3_2:43149380-43149399 | Msa0406440:intron | 45.0% |
| GAAAGTGACAAAGCAGTCGT+TGG | + | chr3_2:43149604-43149623 | Msa0406440:CDS | 45.0% | |
| ATGGGTGTGCATCAGAACTA+TGG | - | chr3_2:43148544-43148563 | None:intergenic | 45.0% | |
| GATGCACACCCATCAAAGAA+TGG | + | chr3_2:43148551-43148570 | Msa0406440:CDS | 45.0% | |
| GCAACTCGCCATTCTTTGAT+GGG | - | chr3_2:43148562-43148581 | None:intergenic | 45.0% | |
| TGCAACTCGCCATTCTTTGA+TGG | - | chr3_2:43148563-43148582 | None:intergenic | 45.0% | |
| CGAGTTGCATACTCTTTGTC+AGG | + | chr3_2:43148574-43148593 | Msa0406440:CDS | 45.0% | |
| TGCCAAGTATTCCTCGAAGA+TGG | + | chr3_2:43148813-43148832 | Msa0406440:intron | 45.0% | |
| GACCATCTTCGAGGAATACT+TGG | - | chr3_2:43148818-43148837 | None:intergenic | 45.0% | |
| TAGGATTGCGTAGATGGTAG+AGG | - | chr3_2:43148840-43148859 | None:intergenic | 45.0% | |
| TCAGAGTAGGATTGCGTAGA+TGG | - | chr3_2:43148846-43148865 | None:intergenic | 45.0% | |
| GCAAAGCAAATGGTGCACTA+TGG | - | chr3_2:43148929-43148948 | None:intergenic | 45.0% | |
| CACTAGAATTTCGCACCTAC+TGG | - | chr3_2:43149293-43149312 | None:intergenic | 45.0% | |
| ! | ACTAGGAGTGTAGCATTCCA+CGG | + | chr3_2:43149380-43149399 | Msa0406440:intron | 45.0% |
| GAAAGTGACAAAGCAGTCGT+TGG | + | chr3_2:43149604-43149623 | Msa0406440:CDS | 45.0% | |
| TCGAGGAATACTTGGCACGA+CGG | - | chr3_2:43148810-43148829 | None:intergenic | 50.0% | |
| ATGGTAGAGGACCATCTTCG+AGG | - | chr3_2:43148827-43148846 | None:intergenic | 50.0% | |
| !! | CTTGCCGACTTACACACAGA+TGG | + | chr3_2:43149162-43149181 | Msa0406440:intron | 50.0% |
| AGCTGTTCTCTCATGCCAGT+AGG | + | chr3_2:43149275-43149294 | Msa0406440:CDS | 50.0% | |
| TCGAGGAATACTTGGCACGA+CGG | - | chr3_2:43148810-43148829 | None:intergenic | 50.0% | |
| ATGGTAGAGGACCATCTTCG+AGG | - | chr3_2:43148827-43148846 | None:intergenic | 50.0% | |
| !! | CTTGCCGACTTACACACAGA+TGG | + | chr3_2:43149162-43149181 | Msa0406440:intron | 50.0% |
| AGCTGTTCTCTCATGCCAGT+AGG | + | chr3_2:43149275-43149294 | Msa0406440:CDS | 50.0% | |
| AGTGTAGCATTCCACGGTGC+AGG | + | chr3_2:43149386-43149405 | Msa0406440:intron | 55.0% | |
| ATAACAGCACACCTGCACCG+TGG | - | chr3_2:43149400-43149419 | None:intergenic | 55.0% | |
| AGTGTAGCATTCCACGGTGC+AGG | + | chr3_2:43149386-43149405 | Msa0406440:intron | 55.0% | |
| ATAACAGCACACCTGCACCG+TGG | - | chr3_2:43149400-43149419 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 43148539 | 43149630 | 43148539 | ID=Msa0406440;Name=Msa0406440 |
| chr3_2 | mRNA | 43148539 | 43149630 | 43148539 | ID=Msa0406440-mRNA-1;Parent=Msa0406440;Name=Msa0406440-mRNA-1;_AED=0.41;_eAED=0.55;_QI=0|0|0|0.75|0.33|0.25|4|0|100 |
| chr3_2 | exon | 43148539 | 43148595 | 43148539 | ID=Msa0406440-mRNA-1:exon:8274;Parent=Msa0406440-mRNA-1 |
| chr3_2 | exon | 43148682 | 43148726 | 43148682 | ID=Msa0406440-mRNA-1:exon:8275;Parent=Msa0406440-mRNA-1 |
| chr3_2 | exon | 43149181 | 43149309 | 43149181 | ID=Msa0406440-mRNA-1:exon:8276;Parent=Msa0406440-mRNA-1 |
| chr3_2 | exon | 43149562 | 43149630 | 43149562 | ID=Msa0406440-mRNA-1:exon:8277;Parent=Msa0406440-mRNA-1 |
| chr3_2 | CDS | 43148539 | 43148595 | 43148539 | ID=Msa0406440-mRNA-1:cds;Parent=Msa0406440-mRNA-1 |
| chr3_2 | CDS | 43148682 | 43148726 | 43148682 | ID=Msa0406440-mRNA-1:cds;Parent=Msa0406440-mRNA-1 |
| chr3_2 | CDS | 43149181 | 43149309 | 43149181 | ID=Msa0406440-mRNA-1:cds;Parent=Msa0406440-mRNA-1 |
| chr3_2 | CDS | 43149562 | 43149630 | 43149562 | ID=Msa0406440-mRNA-1:cds;Parent=Msa0406440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
