Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0411030 | AES61869.1 | 76.316 | 38 | 9 | 0 | 29 | 66 | 2 | 39 | 4.88e-12 | 67.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0411030 | G7I2W4 | 76.316 | 38 | 9 | 0 | 29 | 66 | 2 | 39 | 2.33e-12 | 67.8 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0411030 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCAACGTTGGCTTCTCATA+TGG | 0.366294 | 3_2:-53107907 | None:intergenic |
| ATCTGTTGCTACAAAGTTAG+TGG | 0.469036 | 3_2:+53107562 | Msa0411030:CDS |
| CCAACGTTGCGTAACGCTTG+TGG | 0.485051 | 3_2:+53107919 | Msa0411030:CDS |
| AGCGTGGATGCATCGTATAT+TGG | 0.519278 | 3_2:+53107537 | Msa0411030:CDS |
| ATCAAGGAAATTACACTCGA+TGG | 0.538544 | 3_2:-53107587 | None:intergenic |
| TTGTGACATATACTTAATCA+AGG | 0.541954 | 3_2:-53107603 | None:intergenic |
| GAAACTCAAGTTTGGCGCAA+AGG | 0.544030 | 3_2:+53107883 | Msa0411030:intron |
| CCACAAGCGTTACGCAACGT+TGG | 0.634587 | 3_2:-53107919 | None:intergenic |
| TTGATTAAGTATATGTCACA+AGG | 0.651875 | 3_2:+53107605 | Msa0411030:CDS |
| GTCAAGAATCAAATGCAGCG+TGG | 0.701502 | 3_2:+53107521 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATATTTTATATGATTACA+TGG | + | chr3_2:53107852-53107871 | Msa0411030:intron | 10.0% |
| !!! | ATATTCAAAACAATTTTTGA+TGG | + | chr3_2:53107729-53107748 | Msa0411030:intron | 15.0% |
| !! | TTTATTATTGCATATCGATA+TGG | + | chr3_2:53107652-53107671 | Msa0411030:intron | 20.0% |
| !! | ATTATCATATTCATCAACTT+TGG | + | chr3_2:53107807-53107826 | Msa0411030:intron | 20.0% |
| ! | TTGTGACATATACTTAATCA+AGG | - | chr3_2:53107606-53107625 | None:intergenic | 25.0% |
| ! | TTGATTAAGTATATGTCACA+AGG | + | chr3_2:53107605-53107624 | Msa0411030:CDS | 25.0% |
| ! | ATAAAAACGTGTTAGAGATA+TGG | + | chr3_2:53107695-53107714 | Msa0411030:intron | 25.0% |
| ! | TAAAAACGTGTTAGAGATAT+GGG | + | chr3_2:53107696-53107715 | Msa0411030:intron | 25.0% |
| !! | TTTTAACAGAAACTCAAGTT+TGG | + | chr3_2:53107875-53107894 | Msa0411030:intron | 25.0% |
| !! | TTTCAGGAAATAGTTTTCAA+GGG | - | chr3_2:53107968-53107987 | None:intergenic | 25.0% |
| AAAAACGTGTTAGAGATATG+GGG | + | chr3_2:53107697-53107716 | Msa0411030:intron | 30.0% | |
| ! | CTTTCAGGAAATAGTTTTCA+AGG | - | chr3_2:53107969-53107988 | None:intergenic | 30.0% |
| ATCTGTTGCTACAAAGTTAG+TGG | + | chr3_2:53107562-53107581 | Msa0411030:CDS | 35.0% | |
| ATCAAGGAAATTACACTCGA+TGG | - | chr3_2:53107590-53107609 | None:intergenic | 35.0% | |
| TCACAAGGTATGTGTCTTAA+TGG | + | chr3_2:53107620-53107639 | Msa0411030:intron | 35.0% | |
| ! | GTCTGATGACAGATTTTTCT+GGG | - | chr3_2:53107996-53108015 | None:intergenic | 35.0% |
| !! | TTTATGCGAGCTCTAGGTTT+AGG | - | chr3_2:53107680-53107699 | None:intergenic | 40.0% |
| !! | ACGCTTGTGGATTGCATTTT+AGG | + | chr3_2:53107932-53107951 | Msa0411030:CDS | 40.0% |
| !! | CGCTTGTGGATTGCATTTTA+GGG | + | chr3_2:53107933-53107952 | Msa0411030:CDS | 40.0% |
| !!! | GGATTGCATTTTAGGGCTAA+AGG | + | chr3_2:53107940-53107959 | Msa0411030:CDS | 40.0% |
| ! | ATTTTTCTGGGCTCTCTTTC+AGG | - | chr3_2:53107984-53108003 | None:intergenic | 40.0% |
| ! | GGTCTGATGACAGATTTTTC+TGG | - | chr3_2:53107997-53108016 | None:intergenic | 40.0% |
| AGCGTGGATGCATCGTATAT+TGG | + | chr3_2:53107537-53107556 | Msa0411030:CDS | 45.0% | |
| ! | CACGTTTTTATGCGAGCTCT+AGG | - | chr3_2:53107686-53107705 | None:intergenic | 45.0% |
| GAAACTCAAGTTTGGCGCAA+AGG | + | chr3_2:53107883-53107902 | Msa0411030:intron | 45.0% | |
| !! | CGCAACGTTGGCTTCTCATA+TGG | - | chr3_2:53107910-53107929 | None:intergenic | 50.0% |
| CCACAAGCGTTACGCAACGT+TGG | - | chr3_2:53107922-53107941 | None:intergenic | 55.0% | |
| CCAACGTTGCGTAACGCTTG+TGG | + | chr3_2:53107919-53107938 | Msa0411030:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 53107533 | 53108020 | 53107533 | ID=Msa0411030;Name=Msa0411030 |
| chr3_2 | mRNA | 53107533 | 53108020 | 53107533 | ID=Msa0411030-mRNA-1;Parent=Msa0411030;Name=Msa0411030-mRNA-1;_AED=0.29;_eAED=0.39;_QI=0|0|0|1|1|1|2|0|76 |
| chr3_2 | exon | 53107533 | 53107626 | 53107533 | ID=Msa0411030-mRNA-1:exon:10537;Parent=Msa0411030-mRNA-1 |
| chr3_2 | exon | 53107884 | 53108020 | 53107884 | ID=Msa0411030-mRNA-1:exon:10538;Parent=Msa0411030-mRNA-1 |
| chr3_2 | CDS | 53107533 | 53107626 | 53107533 | ID=Msa0411030-mRNA-1:cds;Parent=Msa0411030-mRNA-1 |
| chr3_2 | CDS | 53107884 | 53108020 | 53107884 | ID=Msa0411030-mRNA-1:cds;Parent=Msa0411030-mRNA-1 |
| Gene Sequence |
| Protein sequence |
