AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0411380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0411380 MtrunA17_Chr8g0378831 51.628 215 40 3 1 151 1 215 2.79e-67 208
Msa0411380 MtrunA17_Chr3g0141871 50.698 215 42 3 1 151 1 215 5.70e-63 197
Msa0411380 MtrunA17_Chr3g0102571 49.091 165 26 2 45 151 99 263 3.14e-44 150
Msa0411380 MtrunA17_Chr5g0397261 60.909 110 32 2 52 151 115 223 5.64e-40 138
Msa0411380 MtrunA17_Chr4g0030231 60.000 110 33 2 52 151 114 222 6.91e-40 137
Msa0411380 MtrunA17_Chr7g0248461 60.909 110 32 2 52 151 112 220 8.13e-40 137
Msa0411380 MtrunA17_Chr8g0348481 60.000 110 33 2 52 151 111 219 1.49e-39 137
Msa0411380 MtrunA17_Chr8g0387401 58.772 114 36 2 48 151 122 234 4.55e-39 136
Msa0411380 MtrunA17_Chr4g0045841 56.757 111 37 2 51 151 128 237 7.82e-38 132
Msa0411380 MtrunA17_Chr4g0030151 55.455 110 38 2 52 151 113 221 1.14e-35 127
Msa0411380 MtrunA17_Chr3g0102601 54.701 117 26 3 45 151 2 101 5.50e-35 122
Msa0411380 MtrunA17_Chr4g0065851 50.980 102 37 3 52 140 99 200 9.19e-27 104
Msa0411380 MtrunA17_Chr6g0482681 50.980 102 37 3 52 140 163 264 1.51e-26 104
Msa0411380 MtrunA17_Chr8g0359091 49.020 102 39 3 52 140 100 201 1.56e-26 104
Msa0411380 MtrunA17_Chr2g0287271 50.000 102 38 3 52 140 99 200 1.61e-26 103
Msa0411380 MtrunA17_Chr3g0096631 48.039 102 40 2 52 140 99 200 2.29e-26 103
Msa0411380 MtrunA17_Chr5g0443351 47.059 102 41 2 52 140 96 197 1.04e-25 101
Msa0411380 MtrunA17_Chr2g0314321 46.078 102 42 2 52 140 99 200 1.45e-25 101
Msa0411380 MtrunA17_Chr8g0392811 49.020 102 39 3 52 140 191 292 2.84e-25 100
Msa0411380 MtrunA17_Chr4g0030241 60.759 79 20 2 83 151 6 83 7.59e-25 94.4
Msa0411380 MtrunA17_Chr8g0377071 40.741 108 53 2 55 151 459 566 6.91e-19 82.4
Msa0411380 MtrunA17_Chr4g0057791 40.187 107 53 2 56 151 295 401 2.24e-18 80.9
Msa0411380 MtrunA17_Chr1g0201571 40.367 109 53 3 55 151 200 308 4.53e-18 80.1
Msa0411380 MtrunA17_Chr3g0122781 38.053 113 59 2 50 151 485 597 7.15e-18 79.7
Msa0411380 MtrunA17_Chr8g0339221 39.450 109 53 4 54 151 74 180 1.25e-17 78.6
Msa0411380 MtrunA17_Chr4g0061141 36.134 119 62 4 45 151 171 287 5.20e-17 77.0
Msa0411380 MtrunA17_Chr4g0040141 39.024 123 53 5 45 151 195 311 1.23e-16 75.9
Msa0411380 MtrunA17_Chr2g0317501 38.843 121 56 5 45 151 200 316 2.21e-16 75.1
Msa0411380 MtrunA17_Chr8g0383451 41.284 109 52 3 55 151 72 180 2.42e-16 75.1
Msa0411380 MtrunA17_Chr5g0396361 38.211 123 54 5 45 151 206 322 3.56e-16 74.7
Msa0411380 MtrunA17_Chr7g0218071 36.036 111 59 3 53 151 192 302 5.65e-16 73.9
Msa0411380 MtrunA17_Chr6g0453711 37.615 109 56 3 55 151 184 292 6.76e-16 73.9
Msa0411380 MtrunA17_Chr3g0131861 39.837 123 52 5 45 151 163 279 7.68e-16 73.6
Msa0411380 MtrunA17_Chr2g0324461 39.024 123 53 5 45 151 207 323 8.29e-16 73.6
Msa0411380 MtrunA17_Chr4g0051261 37.500 112 57 3 53 151 74 185 1.80e-15 71.6
Msa0411380 MtrunA17_Chr4g0002001 35.821 134 68 4 35 151 70 202 2.03e-15 71.6
Msa0411380 MtrunA17_Chr6g0483151 35.652 115 60 3 51 151 79 193 5.30e-15 71.2
Msa0411380 MtrunA17_Chr2g0293491 37.500 112 57 3 53 151 74 185 2.30e-14 68.6
Msa0411380 MtrunA17_Chr5g0409421 36.697 109 54 5 55 151 75 180 5.06e-14 68.2
Msa0411380 MtrunA17_Chr3g0102581 70.000 40 12 0 47 86 76 115 6.22e-14 66.6
Msa0411380 MtrunA17_Chr8g0378861 56.140 57 15 1 62 108 1 57 6.78e-14 62.8
Msa0411380 MtrunA17_Chr7g0273601 37.398 123 55 6 45 151 173 289 8.41e-14 67.8
Msa0411380 MtrunA17_Chr3g0093571 35.455 110 57 4 55 151 113 221 2.81e-13 66.2
Msa0411380 MtrunA17_Chr4g0066691 35.294 119 63 4 45 151 170 286 3.19e-13 66.2
Msa0411380 MtrunA17_Chr8g0369451 33.945 109 57 5 55 151 75 180 4.31e-13 65.9
Msa0411380 MtrunA17_Chr3g0099951 34.513 113 57 4 53 151 86 195 6.53e-13 65.1
Msa0411380 MtrunA17_Chr3g0093391 34.545 110 58 4 55 151 111 219 8.79e-13 64.7
Msa0411380 MtrunA17_Chr1g0191221 36.697 109 57 3 55 151 196 304 9.92e-13 64.7
Msa0411380 MtrunA17_Chr8g0378851 52.174 46 22 0 1 46 1 46 5.62e-12 58.5
Msa0411380 MtrunA17_Chr3g0102611 55.556 45 20 0 1 45 1 45 4.02e-11 56.2
Msa0411380 MtrunA17_Chr3g0130781 33.051 118 58 4 55 151 95 212 4.28e-11 60.1
Msa0411380 MtrunA17_Chr8g0350311 41.667 84 36 3 80 151 239 321 4.76e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0411380 AT1G10210.2 49.767 215 44 3 1 151 1 215 1.68e-63 199
Msa0411380 AT1G10210.3 49.767 215 44 3 1 151 1 215 1.68e-63 199
Msa0411380 AT1G10210.1 49.767 215 44 3 1 151 1 215 1.68e-63 199
Msa0411380 AT2G18170.1 49.767 215 44 3 1 151 1 215 4.27e-63 198
Msa0411380 AT1G59580.2 48.837 215 46 3 1 151 1 215 2.16e-62 196
Msa0411380 AT1G59580.1 48.837 215 46 3 1 151 1 215 2.16e-62 196
Msa0411380 AT4G36450.1 47.442 215 46 4 1 151 1 212 2.53e-57 183
Msa0411380 AT1G07880.1 60.000 110 33 2 52 151 107 215 5.60e-41 138
Msa0411380 AT4G01370.1 60.000 110 33 2 52 151 117 225 3.02e-40 139
Msa0411380 AT1G07880.2 60.000 110 33 2 52 151 107 215 5.31e-40 138
Msa0411380 AT1G01560.4 59.091 110 34 2 52 151 114 222 6.24e-39 133
Msa0411380 AT1G01560.1 59.091 110 34 2 52 151 114 222 6.24e-39 133
Msa0411380 AT1G01560.3 59.091 110 34 2 52 151 114 222 1.93e-38 134
Msa0411380 AT1G01560.2 59.091 110 34 2 52 151 114 222 3.25e-38 134
Msa0411380 AT2G43790.1 56.757 111 37 2 51 151 136 245 5.57e-38 134
Msa0411380 AT4G11330.1 57.018 114 38 2 48 151 113 225 1.11e-37 132
Msa0411380 AT3G45640.1 40.404 198 52 5 19 151 24 220 2.13e-37 131
Msa0411380 AT2G46070.2 52.727 110 41 2 52 151 115 223 2.32e-33 120
Msa0411380 AT2G46070.1 52.727 110 41 2 52 151 115 223 6.43e-33 120
Msa0411380 AT2G46070.3 52.727 110 41 2 52 151 149 257 1.08e-32 120
Msa0411380 AT3G59790.1 51.351 111 43 2 51 151 133 242 1.78e-31 116
Msa0411380 AT2G42880.1 50.000 102 38 3 52 140 99 200 2.22e-26 104
Msa0411380 AT2G42880.2 50.000 102 38 3 52 140 99 200 2.31e-26 103
Msa0411380 AT3G18040.2 49.020 102 39 3 52 140 9 110 2.97e-26 102
Msa0411380 AT3G14720.2 44.737 114 49 4 52 151 99 212 3.85e-26 102
Msa0411380 AT3G14720.3 44.737 114 49 4 52 151 99 212 3.85e-26 102
Msa0411380 AT3G14720.1 44.737 114 49 4 52 151 99 212 3.95e-26 103
Msa0411380 AT3G18040.5 49.020 102 39 3 52 140 92 193 4.15e-26 103
Msa0411380 AT3G18040.1 49.020 102 39 3 52 140 97 198 4.24e-26 103
Msa0411380 AT1G73670.1 50.000 102 38 3 52 140 164 265 4.44e-26 103
Msa0411380 AT5G19010.1 49.020 102 39 3 52 140 99 200 4.91e-26 103
Msa0411380 AT3G18040.4 49.020 102 39 3 52 140 206 307 6.92e-26 102
Msa0411380 AT3G18040.3 49.020 102 39 3 52 140 206 307 7.52e-26 102
Msa0411380 AT1G18150.3 49.020 102 39 3 52 140 178 279 7.78e-26 102
Msa0411380 AT1G18150.2 49.020 102 39 3 52 140 178 279 7.78e-26 102
Msa0411380 AT1G18150.1 49.020 102 39 3 52 140 178 279 7.78e-26 102
Msa0411380 AT1G53510.1 47.059 102 41 3 52 140 99 200 1.70e-25 101
Msa0411380 AT2G01450.3 47.059 102 41 2 52 140 90 191 5.85e-25 99.8
Msa0411380 AT2G01450.1 47.059 102 41 2 52 140 90 191 5.85e-25 99.8
Msa0411380 AT2G01450.2 47.059 102 41 2 52 140 90 191 5.85e-25 99.8
Msa0411380 AT2G01450.4 47.059 102 41 2 52 140 90 191 5.85e-25 99.8
Msa0411380 AT5G63370.7 39.450 109 55 2 54 151 375 483 1.70e-18 81.6
Msa0411380 AT5G63370.8 39.450 109 55 2 54 151 375 483 1.70e-18 81.6
Msa0411380 AT5G63370.4 39.450 109 55 2 54 151 375 483 1.70e-18 81.6
Msa0411380 AT5G63370.6 39.450 109 55 2 54 151 375 483 1.70e-18 81.6
Msa0411380 AT5G63370.1 39.450 109 55 2 54 151 375 483 1.70e-18 81.6
Msa0411380 AT5G63370.5 39.450 109 55 2 54 151 375 483 1.75e-18 81.6
Msa0411380 AT5G63370.10 39.450 109 55 2 54 151 375 483 1.75e-18 81.6
Msa0411380 AT5G63370.9 39.450 109 55 2 54 151 241 349 1.81e-18 81.3
Msa0411380 AT5G63370.2 39.450 109 55 2 54 151 241 349 1.89e-18 81.3
Msa0411380 AT5G63370.3 39.450 109 55 2 54 151 241 349 1.89e-18 81.3
Msa0411380 AT4G13020.1 40.367 109 52 4 54 151 74 180 3.96e-18 80.5
Msa0411380 AT4G13020.5 40.367 109 52 4 54 151 83 189 4.30e-18 80.5
Msa0411380 AT4G13020.2 40.367 109 52 4 54 151 82 188 4.73e-18 80.1
Msa0411380 AT4G13020.3 40.367 109 52 4 54 151 82 188 4.74e-18 80.1
Msa0411380 AT4G13020.6 40.367 109 52 4 54 151 74 180 4.98e-18 80.1
Msa0411380 AT4G13020.4 40.367 109 52 4 54 151 74 180 5.32e-18 80.1
Msa0411380 AT4G13020.7 40.367 109 52 4 54 151 74 180 6.16e-18 79.7
Msa0411380 AT4G13020.8 40.367 109 52 4 54 151 83 189 6.59e-18 79.7
Msa0411380 AT1G67580.1 38.889 108 55 2 55 151 480 587 4.25e-17 77.8
Msa0411380 AT1G67580.2 38.889 108 55 2 55 151 480 587 4.25e-17 77.8
Msa0411380 AT1G54610.1 39.669 121 55 5 45 151 184 300 3.10e-16 75.1
Msa0411380 AT1G54610.3 39.669 121 55 5 45 151 184 300 3.10e-16 75.1
Msa0411380 AT1G54610.2 39.669 121 55 5 45 151 184 300 3.10e-16 75.1
Msa0411380 AT2G38620.4 33.571 140 72 4 31 151 3 140 3.55e-16 73.6
Msa0411380 AT1G18670.1 35.833 120 62 4 45 151 197 314 4.10e-16 74.7
Msa0411380 AT1G18670.2 35.833 120 62 4 45 151 197 314 7.23e-16 74.3
Msa0411380 AT5G50860.1 38.333 120 58 5 45 151 180 296 7.34e-16 73.9
Msa0411380 AT1G18670.3 35.833 120 62 4 45 151 197 314 7.40e-16 74.3
Msa0411380 AT1G53050.2 39.024 123 53 5 45 151 200 316 9.64e-16 73.9
Msa0411380 AT1G53050.1 39.024 123 53 5 45 151 200 316 9.64e-16 73.9
Msa0411380 AT5G50860.2 38.333 120 58 5 45 151 180 296 1.34e-15 73.2
Msa0411380 AT1G71530.4 40.351 114 48 4 54 151 220 329 1.61e-15 72.8
Msa0411380 AT1G71530.1 40.708 113 47 4 55 151 221 329 1.61e-15 73.2
Msa0411380 AT1G74330.3 34.711 121 63 4 45 151 187 305 1.72e-15 73.2
Msa0411380 AT1G74330.2 34.711 121 63 4 45 151 187 305 1.73e-15 73.2
Msa0411380 AT1G74330.1 34.711 121 63 4 45 151 187 305 1.74e-15 73.2
Msa0411380 AT1G71530.2 40.351 114 48 4 54 151 220 329 2.12e-15 72.8
Msa0411380 AT1G71530.3 40.351 114 48 4 54 151 220 329 2.21e-15 72.8
Msa0411380 AT2G38620.3 36.522 115 56 3 54 151 88 202 3.58e-15 70.9
Msa0411380 AT2G38620.1 36.522 115 56 3 54 151 88 202 3.58e-15 70.9
Msa0411380 AT5G44290.7 41.593 113 46 4 55 151 211 319 4.67e-15 71.6
Msa0411380 AT1G73690.1 35.455 110 60 2 53 151 81 190 5.20e-15 71.6
Msa0411380 AT2G38620.2 36.522 115 56 3 54 151 88 202 5.21e-15 70.9
Msa0411380 AT5G44290.6 41.593 113 46 4 55 151 211 319 1.32e-14 70.5
Msa0411380 AT5G44290.2 41.593 113 46 4 55 151 211 319 1.80e-14 70.1
Msa0411380 AT5G44290.5 41.593 113 46 4 55 151 211 319 1.80e-14 70.1
Msa0411380 AT5G44290.4 41.593 113 46 4 55 151 211 319 1.80e-14 70.1
Msa0411380 AT5G44290.1 41.593 113 46 4 55 151 211 319 1.80e-14 70.1
Msa0411380 AT5G44290.3 41.593 113 46 4 55 151 211 319 1.80e-14 70.1
Msa0411380 AT4G10010.2 37.615 109 56 3 55 151 230 338 1.84e-14 70.1
Msa0411380 AT3G48750.1 36.607 112 58 3 53 151 74 185 2.02e-14 69.3
Msa0411380 AT4G10010.1 37.615 109 56 3 55 151 50 158 2.13e-14 69.7
Msa0411380 AT1G03740.2 38.053 113 50 4 55 151 287 395 3.16e-14 69.3
Msa0411380 AT1G03740.1 38.053 113 50 4 55 151 287 395 3.19e-14 69.3
Msa0411380 AT1G18040.1 34.545 110 61 2 53 151 82 191 7.82e-14 68.2
Msa0411380 AT1G33770.1 38.938 113 49 4 55 151 215 323 8.01e-14 68.2
Msa0411380 AT3G54180.1 34.146 123 64 2 46 151 78 200 9.12e-14 67.4
Msa0411380 AT5G39420.1 36.937 111 54 4 55 151 179 287 1.05e-13 67.8
Msa0411380 AT3G01085.1 34.862 109 59 3 55 151 189 297 3.90e-13 66.2
Msa0411380 AT3G01085.2 34.862 109 59 3 55 151 189 297 3.90e-13 66.2
Msa0411380 AT3G01085.3 34.862 109 59 3 55 151 189 297 3.94e-13 66.2
Msa0411380 AT4G22940.2 37.168 113 51 4 55 151 178 286 1.32e-12 64.7
Msa0411380 AT4G22940.1 37.168 113 51 4 55 151 178 286 1.38e-12 64.7
Msa0411380 AT5G10270.1 34.862 109 59 3 55 151 114 222 1.72e-12 64.3
Msa0411380 AT5G64960.1 34.862 109 59 3 55 151 114 222 1.73e-12 64.3
Msa0411380 AT1G66750.1 35.185 108 59 2 55 151 85 192 1.80e-12 63.9
Msa0411380 AT5G64960.2 34.862 109 59 3 55 151 61 169 2.52e-12 63.9
Msa0411380 AT1G76540.1 33.929 112 59 3 55 151 92 203 2.24e-11 60.8
Msa0411380 AT1G20930.1 33.929 112 59 3 55 151 94 205 4.61e-11 60.1

Find 30 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCATTGCCGATATTTCT+TGG 0.281243 3_2:-53605657 Msa0411380:CDS
CGGCAATGGTCATTGGAAAT+TGG 0.300842 3_2:+53605668 None:intergenic
GCTCGGACCAATCAAGTTAA+TGG 0.324476 3_2:-53605160 Msa0411380:CDS
AATCTCGCCATTAACTTGAT+TGG 0.360614 3_2:+53605153 None:intergenic
TTGAGATTGATACGAAATAT+AGG 0.387149 3_2:-53605977 Msa0411380:CDS
CTATTAGCATGTGACAATTA+CGG 0.396118 3_2:-53605085 Msa0411380:CDS
GAAATATCGGCAATGGTCAT+TGG 0.411829 3_2:+53605661 None:intergenic
GTAAAATAATGATTTCCCTT+CGG 0.449033 3_2:+53606017 None:intergenic
CCCTTCGGCTTAATTCTCTC+AGG 0.453879 3_2:+53606032 None:intergenic
CTACTTTGTCTATGAACTCA+TGG 0.455179 3_2:-53605726 Msa0411380:CDS
GTACCTGCGCCAAGAAATAT+CGG 0.461903 3_2:+53605648 None:intergenic
ATAGGCTAATCGAGCAGATT+GGG 0.462759 3_2:-53605959 Msa0411380:CDS
TATAGGCTAATCGAGCAGAT+TGG 0.475038 3_2:-53605960 Msa0411380:CDS
TCACATGCTAATAGAAGCTC+TGG 0.477513 3_2:+53605094 None:intergenic
CAGATTGGGCGAGGGTCGTA+TGG 0.495039 3_2:-53605945 Msa0411380:CDS
CTAATCGAGCAGATTGGGCG+AGG 0.537782 3_2:-53605954 Msa0411380:CDS
TTGCCGATATTTCTTGGCGC+AGG 0.547464 3_2:-53605651 Msa0411380:intron
GCAAGAATGCATCCCACGGA+TGG 0.565016 3_2:+53605057 None:intergenic
TATGTGACTTCGGACTTGCT+CGG 0.583313 3_2:-53605177 Msa0411380:CDS
GCGCCAAGAAATATCGGCAA+TGG 0.597426 3_2:+53605654 None:intergenic
TGGGCGAGGGTCGTATGGCG+TGG 0.601870 3_2:-53605940 Msa0411380:intron
CAGAGTATATTGTCACGCGT+TGG 0.604813 3_2:-53605126 Msa0411380:CDS
CTCAGCAAGAATGCATCCCA+CGG 0.610293 3_2:+53605053 None:intergenic
TCCTGAGAGAATTAAGCCGA+AGG 0.610761 3_2:-53606033 Msa0411380:CDS
GTCATTGGAAATTGGCTCAG+AGG 0.613015 3_2:+53605676 None:intergenic
GATTTGAAGATATGTGACTT+CGG 0.636250 3_2:-53605187 Msa0411380:CDS
CCTGAGAGAATTAAGCCGAA+GGG 0.642509 3_2:-53606032 Msa0411380:CDS
TAATCGAGCAGATTGGGCGA+GGG 0.659462 3_2:-53605953 Msa0411380:CDS
AATTACGGAACATCCATCCG+TGG 0.663559 3_2:-53605070 Msa0411380:CDS
ATTACGGAACATCCATCCGT+GGG 0.671330 3_2:-53605069 Msa0411380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTATTTTATAATTTAGTTG+AGG - chr3_2:53605247-53605266 Msa0411380:CDS 15.0%
!!! ATATTTTCAGTTATTTCTAT+TGG - chr3_2:53605497-53605516 Msa0411380:intron 15.0%
!!! TGTATTTTATAATTTAGTTG+AGG - chr3_2:53605247-53605266 Msa0411380:CDS 15.0%
!!! ATATTTTCAGTTATTTCTAT+TGG - chr3_2:53605497-53605516 Msa0411380:intron 15.0%
!!! GAAATCATTATTTTACAATG+TGG - chr3_2:53605097-53605116 Msa0411380:CDS 20.0%
!! TAGAAATAACTGAAAATATG+AGG + chr3_2:53605496-53605515 None:intergenic 20.0%
!! AGAAAATTATAGCTCATAAT+AGG + chr3_2:53605703-53605722 None:intergenic 20.0%
!! ATTACTATTCCTATTATCAA+TGG + chr3_2:53605771-53605790 None:intergenic 20.0%
!!! GAAATCATTATTTTACAATG+TGG - chr3_2:53605097-53605116 Msa0411380:CDS 20.0%
!! TAGAAATAACTGAAAATATG+AGG + chr3_2:53605496-53605515 None:intergenic 20.0%
!! AGAAAATTATAGCTCATAAT+AGG + chr3_2:53605703-53605722 None:intergenic 20.0%
!! ATTACTATTCCTATTATCAA+TGG + chr3_2:53605771-53605790 None:intergenic 20.0%
! GTAAAATAATGATTTCCCTT+CGG + chr3_2:53605093-53605112 None:intergenic 25.0%
! TTGAGATTGATACGAAATAT+AGG - chr3_2:53605130-53605149 Msa0411380:CDS 25.0%
!!! CTTAAAACAAGCTGTTTTTT+TGG + chr3_2:53605356-53605375 None:intergenic 25.0%
!!! CAAAAAAACAGCTTGTTTTA+AGG - chr3_2:53605354-53605373 Msa0411380:intron 25.0%
! TCAGTCAAAACTCAAATTAA+AGG - chr3_2:53605530-53605549 Msa0411380:intron 25.0%
! AAACTCAAATTAAAGGTAGT+TGG - chr3_2:53605537-53605556 Msa0411380:intron 25.0%
! TTATAGAATGTATGAACTAG+AGG - chr3_2:53605586-53605605 Msa0411380:intron 25.0%
! AACTAGAGGATGAAAATTAT+TGG - chr3_2:53605600-53605619 Msa0411380:intron 25.0%
! GTAAAATAATGATTTCCCTT+CGG + chr3_2:53605093-53605112 None:intergenic 25.0%
! TTGAGATTGATACGAAATAT+AGG - chr3_2:53605130-53605149 Msa0411380:CDS 25.0%
!!! CTTAAAACAAGCTGTTTTTT+TGG + chr3_2:53605356-53605375 None:intergenic 25.0%
!!! CAAAAAAACAGCTTGTTTTA+AGG - chr3_2:53605354-53605373 Msa0411380:intron 25.0%
! TCAGTCAAAACTCAAATTAA+AGG - chr3_2:53605530-53605549 Msa0411380:intron 25.0%
! AAACTCAAATTAAAGGTAGT+TGG - chr3_2:53605537-53605556 Msa0411380:intron 25.0%
! TTATAGAATGTATGAACTAG+AGG - chr3_2:53605586-53605605 Msa0411380:intron 25.0%
! AACTAGAGGATGAAAATTAT+TGG - chr3_2:53605600-53605619 Msa0411380:intron 25.0%
TCACATTAATTTCCCAACTT+GGG + chr3_2:53605626-53605645 None:intergenic 30.0%
! TGTTGATCACCATTGATAAT+AGG - chr3_2:53605759-53605778 Msa0411380:intron 30.0%
GATTTGAAGATATGTGACTT+CGG - chr3_2:53605920-53605939 Msa0411380:intron 30.0%
CTATTAGCATGTGACAATTA+CGG - chr3_2:53606022-53606041 Msa0411380:CDS 30.0%
TCACATTAATTTCCCAACTT+GGG + chr3_2:53605626-53605645 None:intergenic 30.0%
! TGTTGATCACCATTGATAAT+AGG - chr3_2:53605759-53605778 Msa0411380:intron 30.0%
GATTTGAAGATATGTGACTT+CGG - chr3_2:53605920-53605939 Msa0411380:intron 30.0%
CTATTAGCATGTGACAATTA+CGG - chr3_2:53606022-53606041 Msa0411380:CDS 30.0%
! TATCTCCCTTAAAGCTCTAA+GGG + chr3_2:53605273-53605292 None:intergenic 35.0%
TTATCTCCCTTAAAGCTCTA+AGG + chr3_2:53605274-53605293 None:intergenic 35.0%
TAAAGTTGCTTAGACATGTC+CGG - chr3_2:53605291-53605310 Msa0411380:intron 35.0%
CTACTTTGTCTATGAACTCA+TGG - chr3_2:53605381-53605400 Msa0411380:intron 35.0%
TGAAAATTATTGGCCCAAGT+TGG - chr3_2:53605610-53605629 Msa0411380:intron 35.0%
GAAAATTATTGGCCCAAGTT+GGG - chr3_2:53605611-53605630 Msa0411380:intron 35.0%
CTCACATTAATTTCCCAACT+TGG + chr3_2:53605627-53605646 None:intergenic 35.0%
!!! CTCTTATTTTAGCTGCTTGA+AGG - chr3_2:53605851-53605870 Msa0411380:intron 35.0%
! AATCTCGCCATTAACTTGAT+TGG + chr3_2:53605957-53605976 None:intergenic 35.0%
! TATCTCCCTTAAAGCTCTAA+GGG + chr3_2:53605273-53605292 None:intergenic 35.0%
TTATCTCCCTTAAAGCTCTA+AGG + chr3_2:53605274-53605293 None:intergenic 35.0%
TAAAGTTGCTTAGACATGTC+CGG - chr3_2:53605291-53605310 Msa0411380:intron 35.0%
CTACTTTGTCTATGAACTCA+TGG - chr3_2:53605381-53605400 Msa0411380:intron 35.0%
TGAAAATTATTGGCCCAAGT+TGG - chr3_2:53605610-53605629 Msa0411380:intron 35.0%
GAAAATTATTGGCCCAAGTT+GGG - chr3_2:53605611-53605630 Msa0411380:intron 35.0%
CTCACATTAATTTCCCAACT+TGG + chr3_2:53605627-53605646 None:intergenic 35.0%
!!! CTCTTATTTTAGCTGCTTGA+AGG - chr3_2:53605851-53605870 Msa0411380:intron 35.0%
! AATCTCGCCATTAACTTGAT+TGG + chr3_2:53605957-53605976 None:intergenic 35.0%
TATAGGCTAATCGAGCAGAT+TGG - chr3_2:53605147-53605166 Msa0411380:CDS 40.0%
ATAGGCTAATCGAGCAGATT+GGG - chr3_2:53605148-53605167 Msa0411380:CDS 40.0%
TCTTAATTGCGACTTCTCGT+TGG + chr3_2:53605217-53605236 None:intergenic 40.0%
CAAAGTGATCACATATGTGC+CGG + chr3_2:53605313-53605332 None:intergenic 40.0%
GAAATATCGGCAATGGTCAT+TGG + chr3_2:53605449-53605468 None:intergenic 40.0%
TGACCATTGCCGATATTTCT+TGG - chr3_2:53605450-53605469 Msa0411380:intron 40.0%
TCACATGCTAATAGAAGCTC+TGG + chr3_2:53606016-53606035 None:intergenic 40.0%
TATAGGCTAATCGAGCAGAT+TGG - chr3_2:53605147-53605166 Msa0411380:CDS 40.0%
ATAGGCTAATCGAGCAGATT+GGG - chr3_2:53605148-53605167 Msa0411380:CDS 40.0%
TCTTAATTGCGACTTCTCGT+TGG + chr3_2:53605217-53605236 None:intergenic 40.0%
CAAAGTGATCACATATGTGC+CGG + chr3_2:53605313-53605332 None:intergenic 40.0%
GAAATATCGGCAATGGTCAT+TGG + chr3_2:53605449-53605468 None:intergenic 40.0%
TGACCATTGCCGATATTTCT+TGG - chr3_2:53605450-53605469 Msa0411380:intron 40.0%
TCACATGCTAATAGAAGCTC+TGG + chr3_2:53606016-53606035 None:intergenic 40.0%
TCCTGAGAGAATTAAGCCGA+AGG - chr3_2:53605074-53605093 Msa0411380:CDS 45.0%
CCTGAGAGAATTAAGCCGAA+GGG - chr3_2:53605075-53605094 Msa0411380:CDS 45.0%
! CTCGTTGGTCTCCTTATTGA+TGG + chr3_2:53605202-53605221 None:intergenic 45.0%
TTGAGGCCCTTAGAGCTTTA+AGG - chr3_2:53605264-53605283 Msa0411380:intron 45.0%
! TGAGGCCCTTAGAGCTTTAA+GGG - chr3_2:53605265-53605284 Msa0411380:intron 45.0%
! GTCATTGGAAATTGGCTCAG+AGG + chr3_2:53605434-53605453 None:intergenic 45.0%
CGGCAATGGTCATTGGAAAT+TGG + chr3_2:53605442-53605461 None:intergenic 45.0%
GTACCTGCGCCAAGAAATAT+CGG + chr3_2:53605462-53605481 None:intergenic 45.0%
! TATGTGACTTCGGACTTGCT+CGG - chr3_2:53605930-53605949 Msa0411380:intron 45.0%
GCTCGGACCAATCAAGTTAA+TGG - chr3_2:53605947-53605966 Msa0411380:CDS 45.0%
CAGAGTATATTGTCACGCGT+TGG - chr3_2:53605981-53606000 Msa0411380:CDS 45.0%
AATTACGGAACATCCATCCG+TGG - chr3_2:53606037-53606056 Msa0411380:CDS 45.0%
ATTACGGAACATCCATCCGT+GGG - chr3_2:53606038-53606057 Msa0411380:CDS 45.0%
TCCTGAGAGAATTAAGCCGA+AGG - chr3_2:53605074-53605093 Msa0411380:CDS 45.0%
CCTGAGAGAATTAAGCCGAA+GGG - chr3_2:53605075-53605094 Msa0411380:CDS 45.0%
! CTCGTTGGTCTCCTTATTGA+TGG + chr3_2:53605202-53605221 None:intergenic 45.0%
TTGAGGCCCTTAGAGCTTTA+AGG - chr3_2:53605264-53605283 Msa0411380:intron 45.0%
! TGAGGCCCTTAGAGCTTTAA+GGG - chr3_2:53605265-53605284 Msa0411380:intron 45.0%
! GTCATTGGAAATTGGCTCAG+AGG + chr3_2:53605434-53605453 None:intergenic 45.0%
CGGCAATGGTCATTGGAAAT+TGG + chr3_2:53605442-53605461 None:intergenic 45.0%
GTACCTGCGCCAAGAAATAT+CGG + chr3_2:53605462-53605481 None:intergenic 45.0%
! TATGTGACTTCGGACTTGCT+CGG - chr3_2:53605930-53605949 Msa0411380:intron 45.0%
GCTCGGACCAATCAAGTTAA+TGG - chr3_2:53605947-53605966 Msa0411380:CDS 45.0%
CAGAGTATATTGTCACGCGT+TGG - chr3_2:53605981-53606000 Msa0411380:CDS 45.0%
AATTACGGAACATCCATCCG+TGG - chr3_2:53606037-53606056 Msa0411380:CDS 45.0%
ATTACGGAACATCCATCCGT+GGG - chr3_2:53606038-53606057 Msa0411380:CDS 45.0%
CCCTTCGGCTTAATTCTCTC+AGG + chr3_2:53605078-53605097 None:intergenic 50.0%
TAATCGAGCAGATTGGGCGA+GGG - chr3_2:53605154-53605173 Msa0411380:CDS 50.0%
GGTGTGCTCAGCCATCAATA+AGG - chr3_2:53605188-53605207 Msa0411380:CDS 50.0%
GCGCCAAGAAATATCGGCAA+TGG + chr3_2:53605456-53605475 None:intergenic 50.0%
TTGCCGATATTTCTTGGCGC+AGG - chr3_2:53605456-53605475 Msa0411380:intron 50.0%
CCCTTCGGCTTAATTCTCTC+AGG + chr3_2:53605078-53605097 None:intergenic 50.0%
TAATCGAGCAGATTGGGCGA+GGG - chr3_2:53605154-53605173 Msa0411380:CDS 50.0%
GGTGTGCTCAGCCATCAATA+AGG - chr3_2:53605188-53605207 Msa0411380:CDS 50.0%
GCGCCAAGAAATATCGGCAA+TGG + chr3_2:53605456-53605475 None:intergenic 50.0%
TTGCCGATATTTCTTGGCGC+AGG - chr3_2:53605456-53605475 Msa0411380:intron 50.0%
CTAATCGAGCAGATTGGGCG+AGG - chr3_2:53605153-53605172 Msa0411380:CDS 55.0%
GCAAGAATGCATCCCACGGA+TGG + chr3_2:53606053-53606072 None:intergenic 55.0%
CTAATCGAGCAGATTGGGCG+AGG - chr3_2:53605153-53605172 Msa0411380:CDS 55.0%
GCAAGAATGCATCCCACGGA+TGG + chr3_2:53606053-53606072 None:intergenic 55.0%
CAGATTGGGCGAGGGTCGTA+TGG - chr3_2:53605162-53605181 Msa0411380:CDS 60.0%
CAGATTGGGCGAGGGTCGTA+TGG - chr3_2:53605162-53605181 Msa0411380:CDS 60.0%
TGGGCGAGGGTCGTATGGCG+TGG - chr3_2:53605167-53605186 Msa0411380:CDS 70.0%
TGGGCGAGGGTCGTATGGCG+TGG - chr3_2:53605167-53605186 Msa0411380:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 53605054 53606075 53605054 ID=Msa0411380;Name=Msa0411380
chr3_2 mRNA 53605054 53606075 53605054 ID=Msa0411380-mRNA-1;Parent=Msa0411380;Name=Msa0411380-mRNA-1;_AED=0.30;_eAED=0.33;_QI=0|0|0|1|1|1|3|0|157
chr3_2 exon 53605941 53606075 53605941 ID=Msa0411380-mRNA-1:exon:10774;Parent=Msa0411380-mRNA-1
chr3_2 exon 53605652 53605777 53605652 ID=Msa0411380-mRNA-1:exon:10773;Parent=Msa0411380-mRNA-1
chr3_2 exon 53605054 53605266 53605054 ID=Msa0411380-mRNA-1:exon:10772;Parent=Msa0411380-mRNA-1
chr3_2 CDS 53605941 53606075 53605941 ID=Msa0411380-mRNA-1:cds;Parent=Msa0411380-mRNA-1
chr3_2 CDS 53605652 53605777 53605652 ID=Msa0411380-mRNA-1:cds;Parent=Msa0411380-mRNA-1
chr3_2 CDS 53605054 53605266 53605054 ID=Msa0411380-mRNA-1:cds;Parent=Msa0411380-mRNA-1
Gene Sequence

>Msa0411380

ATGGCAACATTAGTGGAACGTCCTGAGAGAATTAAGCCGAAGGGAAATCATTATTTTACAATGTGGAAAACAATATTTGAGATTGATACGAAATATAGGCTAATCGAGCAGATTGGGCGAGGGTCGTATGGCGTGACCAAAAAAACAGCTTGTTTTAAGGATGTCTACTTTGTCTATGAACTCATGGATACTGATCTTCATCGTGTTATCAAGTCCTCTGAGCCAATTTCCAATGACCATTGCCGATATTTCTTGGCGCAGCTGCTTGAAGGTCTTAATCATCTTCATTCTGCAAATTTGCTCATCAATGAAAATTGTGATTTGAAGATATGTGACTTCGGACTTGCTCGGACCAATCAAGTTAATGGCGAGATTATGACAGAGTATATTGTCACGCGTTGGTATCGTGCTCCAGAGCTTCTATTAGCATGTGACAATTACGGAACATCCATCCGTGGGATGCATTCTTGCTGA
Protein sequence

>Msa0411380

MATLVERPERIKPKGNHYFTMWKTIFEIDTKYRLIEQIGRGSYGVTKKTACFKDVYFVYELMDTDLHRVIKSSEPISNDHCRYFLAQLLEGLNHLHSANLLINENCDLKICDFGLARTNQVNGEIMTEYIVTRWYRAPELLLACDNYGTSIRGMHSC*