AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0413420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0413420 MtrunA17_Chr3g0105711 87.729 546 61 5 1 544 1 542 0.0 922
Msa0413420 MtrunA17_Chr3g0136081 49.217 575 196 13 7 543 2 518 9.28e-165 478
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0413420 AT5G16100.2 25.253 297 178 6 267 528 153 440 7.31e-24 105

Find 108 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCAAGTCCATGGTAATTT+TGG 0.219722 3_2:-57860071 None:intergenic
GGGTCCATTATTGCTATTAA+TGG 0.250205 3_2:-57860046 None:intergenic
ATTGCTATTAATGGACAAAT+TGG 0.250376 3_2:-57860037 None:intergenic
CAAAATAATAATGTTTCATT+TGG 0.253731 3_2:+57860612 Msa0413420:CDS
CATGGACATGGTAGTAATAT+TGG 0.259830 3_2:+57860258 Msa0413420:CDS
TTGTCCATTAATAGCAATAA+TGG 0.260366 3_2:+57860042 Msa0413420:CDS
TGGATTGCGGTTGCTCAAAT+TGG 0.290960 3_2:-57860013 None:intergenic
AGACGAATCATTTACTCTAA+TGG 0.306498 3_2:+57860762 Msa0413420:CDS
ATTGTTGGGCCTCCATTAAC+AGG 0.321028 3_2:-57860945 None:intergenic
TATGAAAATCTTGAGATTCA+TGG 0.321881 3_2:+57860882 Msa0413420:CDS
AACGTTGAACCTTCCGTACT+TGG 0.325070 3_2:-57859865 None:intergenic
CAGGAGCAAGTTGGTGAGAT+TGG 0.325114 3_2:+57861792 Msa0413420:CDS
CGCCATGTGACACCTGTTAA+TGG 0.325291 3_2:+57860933 Msa0413420:CDS
TTGGATTGATGATATTAATA+TGG 0.329852 3_2:+57861211 Msa0413420:CDS
CACACTACTGCTGCTGATAA+TGG 0.341115 3_2:-57862431 None:intergenic
CTCTTTGCACCGGATATGAT+TGG 0.357597 3_2:+57861192 Msa0413420:CDS
CTTGAGATTCATGGAAGCTT+GGG 0.357672 3_2:+57860891 Msa0413420:CDS
CATGCTTACAGATGAGATAA+TGG 0.387376 3_2:+57861755 Msa0413420:intron
GATATTAAATTGTAATTGAA+TGG 0.400636 3_2:-57860432 None:intergenic
TGATGAAATAAAGAACTTCC+TGG 0.412875 3_2:+57861097 Msa0413420:CDS
CTATTAATGGACAAATTGGT+TGG 0.419381 3_2:-57860033 None:intergenic
AGCAATACTTGAAAATCAAT+TGG 0.419584 3_2:+57862278 Msa0413420:CDS
TGGAATAAGTAACTTTGAAG+TGG 0.427695 3_2:+57860632 Msa0413420:CDS
CGATTACTTTCATCTACCTA+TGG 0.438638 3_2:+57862054 Msa0413420:CDS
TGTATATATCATTTAGTGTA+GGG 0.456932 3_2:-57859939 None:intergenic
CTTCCTGGATCAGTATTGTA+TGG 0.458075 3_2:+57861112 Msa0413420:CDS
CAATCGTTCGATCAATTGTT+GGG 0.465946 3_2:-57860959 None:intergenic
TCTTGAGATTCATGGAAGCT+TGG 0.468339 3_2:+57860890 Msa0413420:CDS
AGATGTGCACAATTTACATT+GGG 0.476510 3_2:+57862022 Msa0413420:CDS
TCAATCGTTCGATCAATTGT+TGG 0.476535 3_2:-57860960 None:intergenic
ATTCAGTTGTGGCATTGCAT+TGG 0.484885 3_2:-57860298 None:intergenic
CAACAAAATTACCATGGACT+TGG 0.485819 3_2:+57860153 Msa0413420:CDS
GGAAGTTCTTTATTTCATCA+AGG 0.486733 3_2:-57861094 None:intergenic
GGAATAAGTAACTTTGAAGT+GGG 0.489373 3_2:+57860633 Msa0413420:CDS
CACCAAAATTACCATGGACT+TGG 0.489418 3_2:+57860069 Msa0413420:CDS
TCAATTGGACAGGCAAGATC+CGG 0.489751 3_2:+57862293 Msa0413420:CDS
ATCATCAATCCAATCATATC+CGG 0.493113 3_2:-57861201 None:intergenic
TGTATGGAACATAATTCAGC+AGG 0.494565 3_2:+57861128 Msa0413420:CDS
TTGATCAAGTGCCAACGCTT+CGG 0.497530 3_2:-57860691 None:intergenic
CCAATGGTTCAAAATCAACT+TGG 0.499953 3_2:+57860585 Msa0413420:CDS
GTAGAACAAAATTAAGAAGA+TGG 0.504860 3_2:+57862131 Msa0413420:CDS
CTAGCCGCTGCTTCTTCCAT+AGG 0.508372 3_2:-57862070 None:intergenic
CAACAAAGTAGTAATTTCAA+TGG 0.513743 3_2:+57860108 Msa0413420:CDS
AGAGTCCTTTCCCTTGTCGC+CGG 0.514274 3_2:-57862312 None:intergenic
ATGTATATATCATTTAGTGT+AGG 0.514577 3_2:-57859940 None:intergenic
AAGATGTGCACAATTTACAT+TGG 0.515263 3_2:+57862021 Msa0413420:CDS
CCATGTCCATGTTGAATACC+AGG 0.515311 3_2:-57860537 None:intergenic
AAGCGTTGGCACTTGATCAA+TGG 0.517228 3_2:+57860694 Msa0413420:CDS
TCATGGCATTCAACATGGAC+GGG 0.518404 3_2:+57860395 Msa0413420:CDS
AAGAAGATGGCCACACAGAA+AGG 0.518680 3_2:+57862144 Msa0413420:CDS
GATTGATGATATTAATATGG+AGG 0.518874 3_2:+57861214 Msa0413420:CDS
TACTTGAAAATCAATTGGAC+AGG 0.519249 3_2:+57862283 Msa0413420:CDS
ATGGTGCGGCGGCAAGAAGT+TGG 0.529787 3_2:-57862412 None:intergenic
GTTCCATACAATACTGATCC+AGG 0.531567 3_2:-57861115 None:intergenic
ATCATGGCATTCAACATGGA+CGG 0.537136 3_2:+57860394 Msa0413420:CDS
GATACTAAATTGTAATCGAA+TGG 0.539633 3_2:-57860360 None:intergenic
TCATGGTATTCAACAAGGGC+AGG 0.551808 3_2:+57860467 Msa0413420:CDS
AAGATCCGGCGACAAGGGAA+AGG 0.552910 3_2:+57862307 Msa0413420:CDS
GCGACATAATTCAACGCTGT+TGG 0.553307 3_2:-57862390 None:intergenic
ATATCACCAGAAAATGATCA+TGG 0.553458 3_2:+57860450 Msa0413420:CDS
CTCCATTAACAGGTGTCACA+TGG 0.555997 3_2:-57860935 None:intergenic
GACTTGGAGTACTTCAACGA+GGG 0.565289 3_2:+57860085 Msa0413420:CDS
ATGTCACAACAAAATGCTCC+TGG 0.569643 3_2:+57860519 Msa0413420:CDS
CAGGCAAGATCCGGCGACAA+GGG 0.569973 3_2:+57862302 Msa0413420:CDS
GTATCACCAGAAAATGATCA+TGG 0.570985 3_2:+57860378 Msa0413420:CDS
TACATGAATAACAATAACAA+TGG 0.572167 3_2:+57859958 Msa0413420:CDS
CACCAGAGTGTGGTTCAACA+TGG 0.578034 3_2:+57860240 Msa0413420:CDS
AATGATCATGGCATTCAACA+TGG 0.582948 3_2:+57860390 Msa0413420:CDS
ATTGATGATATTAATATGGA+GGG 0.585774 3_2:+57861215 Msa0413420:CDS
GAGCAAGAGCAGGAGCAAGT+TGG 0.586207 3_2:+57861783 Msa0413420:CDS
GGTATTCAACAAGGGCAGGA+TGG 0.589069 3_2:+57860471 Msa0413420:CDS
TCCGTCCGTGTTGAACGCCA+TGG 0.589945 3_2:-57860323 None:intergenic
TTGAGATTCATGGAAGCTTG+GGG 0.597747 3_2:+57860892 Msa0413420:CDS
AATGATCATGGTATTCAACA+AGG 0.598505 3_2:+57860462 Msa0413420:CDS
GTAGAAGTATTCCAAGTCCA+TGG 0.598550 3_2:-57860164 None:intergenic
ACAGGCAAGATCCGGCGACA+AGG 0.599603 3_2:+57862301 Msa0413420:CDS
ACGCCATGGTCATTCAGTTG+TGG 0.599712 3_2:-57860309 None:intergenic
TCAACATCAGACCGAAGCGT+TGG 0.600663 3_2:+57860680 Msa0413420:CDS
AATGGAGCAAGAGCAAGAGC+AGG 0.601208 3_2:+57861773 Msa0413420:CDS
TATTATGAGACTCTTTGCAC+CGG 0.606478 3_2:+57861182 Msa0413420:CDS
CCTGGTATTCAACATGGACA+TGG 0.609981 3_2:+57860537 Msa0413420:CDS
GGCATTCAACATGGACGGGG+TGG 0.611696 3_2:+57860399 Msa0413420:CDS
ACCATGGCGTTCAACACGGA+CGG 0.619182 3_2:+57860322 Msa0413420:CDS
GGACTTGGAATACTTCTACA+AGG 0.619567 3_2:+57860168 Msa0413420:CDS
CATGGCATTCAACATGGACG+GGG 0.620380 3_2:+57860396 Msa0413420:CDS
CAACAACAAAATTCTTGCAG+TGG 0.625689 3_2:+57860726 Msa0413420:CDS
AATGACCATGGCGTTCAACA+CGG 0.633314 3_2:+57860318 Msa0413420:CDS
CAAGACATGATTGATGATTG+TGG 0.634370 3_2:+57862357 Msa0413420:CDS
GTTGAAGTACTCCAAGTCCA+TGG 0.634674 3_2:-57860080 None:intergenic
CATGTGACACCTGTTAATGG+AGG 0.638273 3_2:+57860936 Msa0413420:CDS
TGGACAAATTGGTTGGATTG+CGG 0.639366 3_2:-57860026 None:intergenic
TCTACCTATGGAAGAAGCAG+CGG 0.639381 3_2:+57862066 Msa0413420:CDS
GTCCATGTTGAACCACACTC+TGG 0.639765 3_2:-57860242 None:intergenic
TGCTGCTGATAATGGTGCGG+CGG 0.640455 3_2:-57862423 None:intergenic
GGACCCCACCAAAATTACCA+TGG 0.646800 3_2:+57860063 Msa0413420:CDS
AATGCTCCTGGTATTCAACA+TGG 0.649450 3_2:+57860531 Msa0413420:CDS
CTGTCCCAACAAAATTACCA+TGG 0.655622 3_2:+57860147 Msa0413420:CDS
GGAATACTTCTACAAGGACA+TGG 0.662894 3_2:+57860174 Msa0413420:CDS
TACTGCTGCTGATAATGGTG+CGG 0.670583 3_2:-57862426 None:intergenic
ATGATCATGGTATTCAACAA+GGG 0.672665 3_2:+57860463 Msa0413420:CDS
GTATGGAACATAATTCAGCA+GGG 0.676056 3_2:+57861129 Msa0413420:CDS
ATAAAATTACCTGCTCTGAG+AGG 0.686791 3_2:-57861833 None:intergenic
GACAAGGGAAAGGACTCTAG+AGG 0.686791 3_2:+57862317 Msa0413420:CDS
AGTGTGGTTCAACATGGACA+TGG 0.687520 3_2:+57860246 Msa0413420:CDS
CCAACAAAATCACCAGAGTG+TGG 0.694382 3_2:+57860230 Msa0413420:CDS
ATGCCACAACTGAATGACCA+TGG 0.716852 3_2:+57860306 Msa0413420:CDS
GACATGATTGATGATTGTGG+CGG 0.760229 3_2:+57862360 Msa0413420:CDS
GGACTTGGAGTACTTCAACG+AGG 0.781256 3_2:+57860084 Msa0413420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTATGTATTATTTT+TGG + chr3_2:57862176-57862195 Msa0413420:intron 10.0%
!! GATATTAAATTGTAATTGAA+TGG - chr3_2:57860435-57860454 None:intergenic 15.0%
!! CAAAATAATAATGTTTCATT+TGG + chr3_2:57860612-57860631 Msa0413420:CDS 15.0%
!! AACATATATATCAAAATAAC+TGG - chr3_2:57860835-57860854 None:intergenic 15.0%
!!! GTTTATTTTGATTCTTATAT+AGG + chr3_2:57860852-57860871 Msa0413420:intron 15.0%
!!! TATCATAATGATTTTGATTT+GGG + chr3_2:57861625-57861644 Msa0413420:intron 15.0%
!! TACTCATGTTGAAAAAATTT+GGG - chr3_2:57859918-57859937 None:intergenic 20.0%
!! TTACTCATGTTGAAAAAATT+TGG - chr3_2:57859919-57859938 None:intergenic 20.0%
!! TGTATATATCATTTAGTGTA+GGG - chr3_2:57859942-57859961 None:intergenic 20.0%
!! ATGTATATATCATTTAGTGT+AGG - chr3_2:57859943-57859962 None:intergenic 20.0%
!! TACATGAATAACAATAACAA+TGG + chr3_2:57859958-57859977 Msa0413420:CDS 20.0%
!!! TTTCTTGTAGTGATTAATTT+TGG - chr3_2:57860811-57860830 None:intergenic 20.0%
!!! TTGGATTGATGATATTAATA+TGG + chr3_2:57861211-57861230 Msa0413420:CDS 20.0%
!!! ATTGATGATATTAATATGGA+GGG + chr3_2:57861215-57861234 Msa0413420:CDS 20.0%
!!! CTTTTAGAAATACATTTGTT+TGG + chr3_2:57861481-57861500 Msa0413420:intron 20.0%
!!! GTATCATAATGATTTTGATT+TGG + chr3_2:57861624-57861643 Msa0413420:intron 20.0%
!!! ATCATAATGATTTTGATTTG+GGG + chr3_2:57861626-57861645 Msa0413420:intron 20.0%
!! ATATTGTATGTATTGAATAG+AGG + chr3_2:57861996-57862015 Msa0413420:intron 20.0%
!!! TTTAAGACACGTATTAAATT+AGG - chr3_2:57862224-57862243 None:intergenic 20.0%
!! ATTGGAATGATGATTTTGTT+GGG - chr3_2:57859998-57860017 None:intergenic 25.0%
!! AATTGGAATGATGATTTTGT+TGG - chr3_2:57859999-57860018 None:intergenic 25.0%
!! ATTGCTATTAATGGACAAAT+TGG - chr3_2:57860040-57860059 None:intergenic 25.0%
! TTGTCCATTAATAGCAATAA+TGG + chr3_2:57860042-57860061 Msa0413420:CDS 25.0%
! CAACAAAGTAGTAATTTCAA+TGG + chr3_2:57860108-57860127 Msa0413420:CDS 25.0%
! GATACTAAATTGTAATCGAA+TGG - chr3_2:57860363-57860382 None:intergenic 25.0%
! TATGAAAATCTTGAGATTCA+TGG + chr3_2:57860882-57860901 Msa0413420:CDS 25.0%
! ACAAATATTACACTAAGACT+TGG - chr3_2:57860993-57861012 None:intergenic 25.0%
!! GATTGATGATATTAATATGG+AGG + chr3_2:57861214-57861233 Msa0413420:CDS 25.0%
! AAACCTGAAAAATTATCGAT+AGG - chr3_2:57861242-57861261 None:intergenic 25.0%
!!! CTTTTAGAAATACACTTGTT+TGG + chr3_2:57861297-57861316 Msa0413420:intron 25.0%
!!! CTTTTAGAAATGCATTTGTT+TGG + chr3_2:57861389-57861408 Msa0413420:intron 25.0%
! TTATTAATGTTATTACGTCG+AGG + chr3_2:57861715-57861734 Msa0413420:intron 25.0%
! GTAGAACAAAATTAAGAAGA+TGG + chr3_2:57862131-57862150 Msa0413420:CDS 25.0%
! CACGTATTAAATTAGGAAAA+TGG - chr3_2:57862217-57862236 None:intergenic 25.0%
! CGTGTCTTAAAATTAAGTAT+AGG + chr3_2:57862233-57862252 Msa0413420:intron 25.0%
! AGCAATACTTGAAAATCAAT+TGG + chr3_2:57862278-57862297 Msa0413420:CDS 25.0%
CAGAAAAAACAATCCAAGTA+CGG + chr3_2:57859852-57859871 Msa0413420:CDS 30.0%
GTTGAAAAAATTTGGGTCAA+TGG - chr3_2:57859911-57859930 None:intergenic 30.0%
! TTTTGTTGGGACAAATTTGT+GGG - chr3_2:57859985-57860004 None:intergenic 30.0%
! ATTTTGTTGGGACAAATTTG+TGG - chr3_2:57859986-57860005 None:intergenic 30.0%
! CTATTAATGGACAAATTGGT+TGG - chr3_2:57860036-57860055 None:intergenic 30.0%
! AAGTCCATGGTAATTTTGTT+GGG - chr3_2:57860154-57860173 None:intergenic 30.0%
ATATCACCAGAAAATGATCA+TGG + chr3_2:57860450-57860469 Msa0413420:CDS 30.0%
! TGAATACCATGATCATTTTC+TGG - chr3_2:57860459-57860478 None:intergenic 30.0%
AATGATCATGGTATTCAACA+AGG + chr3_2:57860462-57860481 Msa0413420:CDS 30.0%
ATGATCATGGTATTCAACAA+GGG + chr3_2:57860463-57860482 Msa0413420:CDS 30.0%
!! ATTTTGTTGTGACATTGCAT+GGG - chr3_2:57860514-57860533 None:intergenic 30.0%
! TGAAACATTTTCTCTTCCAA+TGG + chr3_2:57860569-57860588 Msa0413420:CDS 30.0%
! TGGAATAAGTAACTTTGAAG+TGG + chr3_2:57860632-57860651 Msa0413420:CDS 30.0%
!! GGAATAAGTAACTTTGAAGT+GGG + chr3_2:57860633-57860652 Msa0413420:CDS 30.0%
AGACGAATCATTTACTCTAA+TGG + chr3_2:57860762-57860781 Msa0413420:CDS 30.0%
!! TGTATCAGTTTTTCTGAGAA+AGG + chr3_2:57861071-57861090 Msa0413420:intron 30.0%
! GGAAGTTCTTTATTTCATCA+AGG - chr3_2:57861097-57861116 None:intergenic 30.0%
TGATGAAATAAAGAACTTCC+TGG + chr3_2:57861097-57861116 Msa0413420:CDS 30.0%
ATCATCAATCCAATCATATC+CGG - chr3_2:57861204-57861223 None:intergenic 30.0%
CATTTATGTGTGTGACTAAA+TGG - chr3_2:57861434-57861453 None:intergenic 30.0%
AAGATGTGCACAATTTACAT+TGG + chr3_2:57862021-57862040 Msa0413420:CDS 30.0%
AGATGTGCACAATTTACATT+GGG + chr3_2:57862022-57862041 Msa0413420:CDS 30.0%
! AATACTTTTACCTTTCTGTG+TGG - chr3_2:57862157-57862176 None:intergenic 30.0%
! TATAGGTGAAATGTCTTTTG+AGG + chr3_2:57862250-57862269 Msa0413420:intron 30.0%
TACTTGAAAATCAATTGGAC+AGG + chr3_2:57862283-57862302 Msa0413420:CDS 30.0%
AAAAACAATCCAAGTACGGA+AGG + chr3_2:57859856-57859875 Msa0413420:CDS 35.0%
! GGGTCCATTATTGCTATTAA+TGG - chr3_2:57860049-57860068 None:intergenic 35.0%
!! AAGTCCATGGTAATTTTGGT+GGG - chr3_2:57860070-57860089 None:intergenic 35.0%
! CAAGTCCATGGTAATTTTGT+TGG - chr3_2:57860155-57860174 None:intergenic 35.0%
CAACAAAATTACCATGGACT+TGG + chr3_2:57860153-57860172 Msa0413420:CDS 35.0%
CATGGACATGGTAGTAATAT+TGG + chr3_2:57860258-57860277 Msa0413420:CDS 35.0%
GTATCACCAGAAAATGATCA+TGG + chr3_2:57860378-57860397 Msa0413420:CDS 35.0%
! TGAATGCCATGATCATTTTC+TGG - chr3_2:57860387-57860406 None:intergenic 35.0%
AATGATCATGGCATTCAACA+TGG + chr3_2:57860390-57860409 Msa0413420:CDS 35.0%
!! CATTTTGTTGTGACATTGCA+TGG - chr3_2:57860515-57860534 None:intergenic 35.0%
!!! CCAAGTTGATTTTGAACCAT+TGG - chr3_2:57860588-57860607 None:intergenic 35.0%
CCAATGGTTCAAAATCAACT+TGG + chr3_2:57860585-57860604 Msa0413420:CDS 35.0%
!!! GAATTTTGTTGTTGTTCGAG+TGG - chr3_2:57860720-57860739 None:intergenic 35.0%
CAACAACAAAATTCTTGCAG+TGG + chr3_2:57860726-57860745 Msa0413420:CDS 35.0%
CAATCGTTCGATCAATTGTT+GGG - chr3_2:57860962-57860981 None:intergenic 35.0%
TCAATCGTTCGATCAATTGT+TGG - chr3_2:57860963-57860982 None:intergenic 35.0%
TGTATGGAACATAATTCAGC+AGG + chr3_2:57861128-57861147 Msa0413420:CDS 35.0%
GTATGGAACATAATTCAGCA+GGG + chr3_2:57861129-57861148 Msa0413420:CDS 35.0%
TATTATGAGACTCTTTGCAC+CGG + chr3_2:57861182-57861201 Msa0413420:CDS 35.0%
! GGTCCTATCGATAATTTTTC+AGG + chr3_2:57861236-57861255 Msa0413420:CDS 35.0%
GTTTGTCTTCGAAATCATGA+AGG + chr3_2:57861258-57861277 Msa0413420:intron 35.0%
CATGCTTACAGATGAGATAA+TGG + chr3_2:57861755-57861774 Msa0413420:intron 35.0%
AGATTGGAACACCTGAAAAA+AGG + chr3_2:57861808-57861827 Msa0413420:CDS 35.0%
! ATAAAATTACCTGCTCTGAG+AGG - chr3_2:57861836-57861855 None:intergenic 35.0%
TTGAACAATATATACTCGCG+AGG + chr3_2:57861870-57861889 Msa0413420:intron 35.0%
CGATTACTTTCATCTACCTA+TGG + chr3_2:57862054-57862073 Msa0413420:CDS 35.0%
! CAAGACATGATTGATGATTG+TGG + chr3_2:57862357-57862376 Msa0413420:CDS 35.0%
TGGACAAATTGGTTGGATTG+CGG - chr3_2:57860029-57860048 None:intergenic 40.0%
!! AGTCCATGGTAATTTTGGTG+GGG - chr3_2:57860069-57860088 None:intergenic 40.0%
! CAAGTCCATGGTAATTTTGG+TGG - chr3_2:57860071-57860090 None:intergenic 40.0%
CACCAAAATTACCATGGACT+TGG + chr3_2:57860069-57860088 Msa0413420:CDS 40.0%
! CTCCAAGTCCATGGTAATTT+TGG - chr3_2:57860074-57860093 None:intergenic 40.0%
! ATTTTGTTGGGACAGTGGAT+TGG - chr3_2:57860142-57860161 None:intergenic 40.0%
! TGGTAATTTTGTTGGGACAG+TGG - chr3_2:57860147-57860166 None:intergenic 40.0%
CTGTCCCAACAAAATTACCA+TGG + chr3_2:57860147-57860166 Msa0413420:CDS 40.0%
!! GTAGAAGTATTCCAAGTCCA+TGG - chr3_2:57860167-57860186 None:intergenic 40.0%
GGACTTGGAATACTTCTACA+AGG + chr3_2:57860168-57860187 Msa0413420:CDS 40.0%
GGAATACTTCTACAAGGACA+TGG + chr3_2:57860174-57860193 Msa0413420:CDS 40.0%
! CACACTCTGGTGATTTTGTT+GGG - chr3_2:57860232-57860251 None:intergenic 40.0%
ATTCAGTTGTGGCATTGCAT+TGG - chr3_2:57860301-57860320 None:intergenic 40.0%
ATCATGGCATTCAACATGGA+CGG + chr3_2:57860394-57860413 Msa0413420:CDS 40.0%
ATGTCACAACAAAATGCTCC+TGG + chr3_2:57860519-57860538 Msa0413420:CDS 40.0%
AATGCTCCTGGTATTCAACA+TGG + chr3_2:57860531-57860550 Msa0413420:CDS 40.0%
TCTTGAGATTCATGGAAGCT+TGG + chr3_2:57860890-57860909 Msa0413420:CDS 40.0%
CTTGAGATTCATGGAAGCTT+GGG + chr3_2:57860891-57860910 Msa0413420:CDS 40.0%
TTGAGATTCATGGAAGCTTG+GGG + chr3_2:57860892-57860911 Msa0413420:CDS 40.0%
CTTCCTGGATCAGTATTGTA+TGG + chr3_2:57861112-57861131 Msa0413420:CDS 40.0%
GTTCCATACAATACTGATCC+AGG - chr3_2:57861118-57861137 None:intergenic 40.0%
!!! TCTGAGAGGTTCCTTTTTTC+AGG - chr3_2:57861822-57861841 None:intergenic 40.0%
! GACATGATTGATGATTGTGG+CGG + chr3_2:57862360-57862379 Msa0413420:CDS 40.0%
AACGTTGAACCTTCCGTACT+TGG - chr3_2:57859868-57859887 None:intergenic 45.0%
TGGATTGCGGTTGCTCAAAT+TGG - chr3_2:57860016-57860035 None:intergenic 45.0%
! GTTGAAGTACTCCAAGTCCA+TGG - chr3_2:57860083-57860102 None:intergenic 45.0%
! GACTTGGAGTACTTCAACGA+GGG + chr3_2:57860085-57860104 Msa0413420:CDS 45.0%
! CCACACTCTGGTGATTTTGT+TGG - chr3_2:57860233-57860252 None:intergenic 45.0%
CCAACAAAATCACCAGAGTG+TGG + chr3_2:57860230-57860249 Msa0413420:CDS 45.0%
AGTGTGGTTCAACATGGACA+TGG + chr3_2:57860246-57860265 Msa0413420:CDS 45.0%
ATGCCACAACTGAATGACCA+TGG + chr3_2:57860306-57860325 Msa0413420:CDS 45.0%
AATGACCATGGCGTTCAACA+CGG + chr3_2:57860318-57860337 Msa0413420:CDS 45.0%
TCATGGCATTCAACATGGAC+GGG + chr3_2:57860395-57860414 Msa0413420:CDS 45.0%
TCATGGTATTCAACAAGGGC+AGG + chr3_2:57860467-57860486 Msa0413420:CDS 45.0%
CCATGTCCATGTTGAATACC+AGG - chr3_2:57860540-57860559 None:intergenic 45.0%
CCTGGTATTCAACATGGACA+TGG + chr3_2:57860537-57860556 Msa0413420:CDS 45.0%
TTGATCAAGTGCCAACGCTT+CGG - chr3_2:57860694-57860713 None:intergenic 45.0%
!! AAGCGTTGGCACTTGATCAA+TGG + chr3_2:57860694-57860713 Msa0413420:CDS 45.0%
! CTCCATTAACAGGTGTCACA+TGG - chr3_2:57860938-57860957 None:intergenic 45.0%
CATGTGACACCTGTTAATGG+AGG + chr3_2:57860936-57860955 Msa0413420:CDS 45.0%
ATTGTTGGGCCTCCATTAAC+AGG - chr3_2:57860948-57860967 None:intergenic 45.0%
CTCTTTGCACCGGATATGAT+TGG + chr3_2:57861192-57861211 Msa0413420:CDS 45.0%
GGGTTGTGACTGAGTATTTC+TGG + chr3_2:57861646-57861665 Msa0413420:intron 45.0%
AAAAAGGAACCTCTCAGAGC+AGG + chr3_2:57861824-57861843 Msa0413420:CDS 45.0%
TCTACCTATGGAAGAAGCAG+CGG + chr3_2:57862066-57862085 Msa0413420:CDS 45.0%
AAGAAGATGGCCACACAGAA+AGG + chr3_2:57862144-57862163 Msa0413420:CDS 45.0%
TCAATTGGACAGGCAAGATC+CGG + chr3_2:57862293-57862312 Msa0413420:CDS 45.0%
GCGACATAATTCAACGCTGT+TGG - chr3_2:57862393-57862412 None:intergenic 45.0%
!! TACTGCTGCTGATAATGGTG+CGG - chr3_2:57862429-57862448 None:intergenic 45.0%
GGACCCCACCAAAATTACCA+TGG + chr3_2:57860063-57860082 Msa0413420:CDS 50.0%
! GGACTTGGAGTACTTCAACG+AGG + chr3_2:57860084-57860103 Msa0413420:CDS 50.0%
CACCAGAGTGTGGTTCAACA+TGG + chr3_2:57860240-57860259 Msa0413420:CDS 50.0%
GTCCATGTTGAACCACACTC+TGG - chr3_2:57860245-57860264 None:intergenic 50.0%
ACGCCATGGTCATTCAGTTG+TGG - chr3_2:57860312-57860331 None:intergenic 50.0%
CATGGCATTCAACATGGACG+GGG + chr3_2:57860396-57860415 Msa0413420:CDS 50.0%
GGTATTCAACAAGGGCAGGA+TGG + chr3_2:57860471-57860490 Msa0413420:CDS 50.0%
TCAACATCAGACCGAAGCGT+TGG + chr3_2:57860680-57860699 Msa0413420:CDS 50.0%
CGCCATGTGACACCTGTTAA+TGG + chr3_2:57860933-57860952 Msa0413420:CDS 50.0%
AATGGAGCAAGAGCAAGAGC+AGG + chr3_2:57861773-57861792 Msa0413420:CDS 50.0%
!! CAGGAGCAAGTTGGTGAGAT+TGG + chr3_2:57861792-57861811 Msa0413420:CDS 50.0%
AAGCGACAGACTTGTCTGCA+TGG + chr3_2:57861933-57861952 Msa0413420:intron 50.0%
GACAAGGGAAAGGACTCTAG+AGG + chr3_2:57862317-57862336 Msa0413420:CDS 50.0%
ACCATGGCGTTCAACACGGA+CGG + chr3_2:57860322-57860341 Msa0413420:CDS 55.0%
GAGCAAGAGCAGGAGCAAGT+TGG + chr3_2:57861783-57861802 Msa0413420:CDS 55.0%
CTAGCCGCTGCTTCTTCCAT+AGG - chr3_2:57862073-57862092 None:intergenic 55.0%
AAGATCCGGCGACAAGGGAA+AGG + chr3_2:57862307-57862326 Msa0413420:CDS 55.0%
AGAGTCCTTTCCCTTGTCGC+CGG - chr3_2:57862315-57862334 None:intergenic 55.0%
!! TGCTGCTGATAATGGTGCGG+CGG - chr3_2:57862426-57862445 None:intergenic 55.0%
TCCGTCCGTGTTGAACGCCA+TGG - chr3_2:57860326-57860345 None:intergenic 60.0%
GGCATTCAACATGGACGGGG+TGG + chr3_2:57860399-57860418 Msa0413420:CDS 60.0%
ACAGGCAAGATCCGGCGACA+AGG + chr3_2:57862301-57862320 Msa0413420:CDS 60.0%
CAGGCAAGATCCGGCGACAA+GGG + chr3_2:57862302-57862321 Msa0413420:CDS 60.0%
ATGGTGCGGCGGCAAGAAGT+TGG - chr3_2:57862415-57862434 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 57859844 57862449 57859844 ID=Msa0413420;Name=Msa0413420
chr3_2 mRNA 57859844 57862449 57859844 ID=Msa0413420-mRNA-1;Parent=Msa0413420;Name=Msa0413420-mRNA-1;_AED=0.04;_eAED=0.05;_QI=0|0|0|1|1|1|6|0|550
chr3_2 exon 57859844 57860783 57859844 ID=Msa0413420-mRNA-1:exon:11897;Parent=Msa0413420-mRNA-1
chr3_2 exon 57860874 57860982 57860874 ID=Msa0413420-mRNA-1:exon:11898;Parent=Msa0413420-mRNA-1
chr3_2 exon 57861079 57861257 57861079 ID=Msa0413420-mRNA-1:exon:11899;Parent=Msa0413420-mRNA-1
chr3_2 exon 57861766 57861845 57861766 ID=Msa0413420-mRNA-1:exon:11900;Parent=Msa0413420-mRNA-1
chr3_2 exon 57862016 57862165 57862016 ID=Msa0413420-mRNA-1:exon:11901;Parent=Msa0413420-mRNA-1
chr3_2 exon 57862255 57862449 57862255 ID=Msa0413420-mRNA-1:exon:11902;Parent=Msa0413420-mRNA-1
chr3_2 CDS 57859844 57860783 57859844 ID=Msa0413420-mRNA-1:cds;Parent=Msa0413420-mRNA-1
chr3_2 CDS 57860874 57860982 57860874 ID=Msa0413420-mRNA-1:cds;Parent=Msa0413420-mRNA-1
chr3_2 CDS 57861079 57861257 57861079 ID=Msa0413420-mRNA-1:cds;Parent=Msa0413420-mRNA-1
chr3_2 CDS 57861766 57861845 57861766 ID=Msa0413420-mRNA-1:cds;Parent=Msa0413420-mRNA-1
chr3_2 CDS 57862016 57862165 57862016 ID=Msa0413420-mRNA-1:cds;Parent=Msa0413420-mRNA-1
chr3_2 CDS 57862255 57862449 57862255 ID=Msa0413420-mRNA-1:cds;Parent=Msa0413420-mRNA-1
Gene Sequence

>Msa0413420

ATGGCTAACAGAAAAAACAATCCAAGTACGGAAGGTTCAACGTTTAAATTTAGTGATGATGATGCCATTGACCCAAATTTTTTCAACATGAGTAACCCTACACTAAATGATATATACATGAATAACAATAACAATGGACCCACAAATTTGTCCCAACAAAATCATCATTCCAATTTGAGCAACCGCAATCCAACCAATTTGTCCATTAATAGCAATAATGGACCCCACCAAAATTACCATGGACTTGGAGTACTTCAACGAGGGCAACAAAGTAGTAATTTCAATGGTCACTTTTCCAATCCACTGTCCCAACAAAATTACCATGGACTTGGAATACTTCTACAAGGACATGGTAGTAGTAATGAACAATTTCATACTACACTATCCCAACAAAATCACCAGAGTGTGGTTCAACATGGACATGGTAGTAATATTGGAAATGTCTCATTTAATTCCAATGCAATGCCACAACTGAATGACCATGGCGTTCAACACGGACGGAGTAGTAATTACGATCCATTCGATTACAATTTAGTATCACCAGAAAATGATCATGGCATTCAACATGGACGGGGTGGTAATTATGATCCATTCAATTACAATTTAATATCACCAGAAAATGATCATGGTATTCAACAAGGGCAGGATGGTAATAATGATGTATTTTCCCATGCAATGTCACAACAAAATGCTCCTGGTATTCAACATGGACATGGTAGTACAAATGAAACATTTTCTCTTCCAATGGTTCAAAATCAACTTGGTCAGCAAAATAATAATGTTTCATTTGGAATAAGTAACTTTGAAGTGGGTAGTTCGTCACGAATGAATGAGAATCAACATCAGACCGAAGCGTTGGCACTTGATCAATGGCCACTCGAACAACAACAAAATTCTTGCAGTGGTAGTCAAGTTCTTAGACGAATCATTTACTCTAATGGTTTTCAATATGAAAATCTTGAGATTCATGGAAGCTTGGGGTTGATTACACATGCAGTTCGCCATGTGACACCTGTTAATGGAGGCCCAACAATTGATCGAACGATTGATTTTTCTGAGAAAGGCCTTGATGAAATAAAGAACTTCCTGGATCAGTATTGTATGGAACATAATTCAGCAGGGTACTTTCTTATACAAGATTCAATGTGTGCGTATTATGAGACTCTTTGCACCGGATATGATTGGATTGATGATATTAATATGGAGGGTCCTATCGATAATTTTTCAGATGAGATAATGGAGCAAGAGCAAGAGCAGGAGCAAGTTGGTGAGATTGGAACACCTGAAAAAAGGAACCTCTCAGAGCAGAGGAAAAGATGTGCACAATTTACATTGGGTGATTTCTGCGATTACTTTCATCTACCTATGGAAGAAGCAGCGGCTAGAGTGAACATAGCTGCATCAACTCTAAAGAAAATTTGTCGTAGAACAAAATTAAGAAGATGGCCACACAGAAAGGTGAAATGTCTTTTGAGGCAAATAGCAATACTTGAAAATCAATTGGACAGGCAAGATCCGGCGACAAGGGAAAGGACTCTAGAGGAAATTAAGCAGCTTAAACAAGACATGATTGATGATTGTGGCGGACATACACCAACAGCGTTGAATTATGTCGCCAACTTCTTGCCGCCGCACCATTATCAGCAGCAGTAG
Protein sequence

>Msa0413420

MANRKNNPSTEGSTFKFSDDDAIDPNFFNMSNPTLNDIYMNNNNNGPTNLSQQNHHSNLSNRNPTNLSINSNNGPHQNYHGLGVLQRGQQSSNFNGHFSNPLSQQNYHGLGILLQGHGSSNEQFHTTLSQQNHQSVVQHGHGSNIGNVSFNSNAMPQLNDHGVQHGRSSNYDPFDYNLVSPENDHGIQHGRGGNYDPFNYNLISPENDHGIQQGQDGNNDVFSHAMSQQNAPGIQHGHGSTNETFSLPMVQNQLGQQNNNVSFGISNFEVGSSSRMNENQHQTEALALDQWPLEQQQNSCSGSQVLRRIIYSNGFQYENLEIHGSLGLITHAVRHVTPVNGGPTIDRTIDFSEKGLDEIKNFLDQYCMEHNSAGYFLIQDSMCAYYETLCTGYDWIDDINMEGPIDNFSDEIMEQEQEQEQVGEIGTPEKRNLSEQRKRCAQFTLGDFCDYFHLPMEEAAARVNIAASTLKKICRRTKLRRWPHRKVKCLLRQIAILENQLDRQDPATRERTLEEIKQLKQDMIDDCGGHTPTALNYVANFLPPHHYQQQ*