AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0416360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0416360 MtrunA17_Chr7g0217721 61.988 171 15 2 1 121 1 171 2.05e-63 196
Msa0416360 MtrunA17_Chr7g0217711 62.424 165 18 2 1 121 1 165 1.18e-62 189
Msa0416360 MtrunA17_Chr6g0453451 50.314 159 40 2 1 121 2 159 6.47e-43 143
Msa0416360 MtrunA17_Chr4g0060931 52.980 151 31 3 5 116 2 151 3.99e-42 142
Msa0416360 MtrunA17_Chr3g0113041 92.537 67 5 0 1 67 1 67 8.36e-42 132
Msa0416360 MtrunA17_Chr3g0091921 50.667 150 35 2 5 116 2 150 2.41e-39 133
Msa0416360 MtrunA17_Chr3g0113011 79.452 73 15 0 1 73 1 73 3.95e-38 123
Msa0416360 MtrunA17_Chr2g0316971 49.645 141 30 3 17 117 21 160 1.18e-36 128
Msa0416360 MtrunA17_Chr6g0461661 47.586 145 35 3 14 118 13 156 1.22e-36 128
Msa0416360 MtrunA17_Chr6g0484441 50.394 127 25 1 19 107 5 131 2.30e-36 126
Msa0416360 MtrunA17_Chr7g0253011 45.946 148 37 3 11 116 9 155 2.13e-35 124
Msa0416360 MtrunA17_Chr3g0113031 93.333 60 4 0 62 121 21 80 4.68e-35 121
Msa0416360 MtrunA17_Chr1g0147421 48.062 129 26 3 19 106 27 155 3.41e-32 115
Msa0416360 MtrunA17_Chr3g0113001 88.333 60 7 0 62 121 46 105 3.57e-32 114
Msa0416360 MtrunA17_Chr3g0139331 45.312 128 30 2 19 106 11 138 9.66e-31 112
Msa0416360 MtrunA17_Chr3g0144271 43.333 150 43 4 7 115 12 160 2.30e-30 112
Msa0416360 MtrunA17_Chr2g0312321 41.667 132 35 3 16 108 8 136 5.05e-27 101
Msa0416360 MtrunA17_Chr4g0056541 39.695 131 38 1 20 109 8 138 2.31e-26 100
Msa0416360 MtrunA17_Chr2g0309891 40.625 128 36 1 19 106 6 133 5.31e-26 99.4
Msa0416360 MtrunA17_Chr5g0433781 39.695 131 39 1 17 107 4 134 6.87e-26 100
Msa0416360 MtrunA17_Chr8g0346001 37.500 144 49 1 14 116 10 153 6.98e-26 100
Msa0416360 MtrunA17_Chr4g0017921 42.636 129 33 1 20 107 9 137 9.08e-26 99.0
Msa0416360 MtrunA17_Chr4g0018391 38.931 131 39 1 20 109 8 138 9.50e-26 98.6
Msa0416360 MtrunA17_Chr6g0481091 39.695 131 39 1 17 107 4 134 1.14e-25 96.7
Msa0416360 MtrunA17_Chr3g0109821 42.748 131 35 3 17 107 4 134 1.17e-25 100
Msa0416360 MtrunA17_Chr5g0398971 40.152 132 37 2 20 109 8 139 1.32e-25 98.2
Msa0416360 MtrunA17_Chr8g0372121 38.760 129 38 1 20 107 7 135 1.90e-25 97.4
Msa0416360 MtrunA17_Chr2g0309331 38.571 140 44 2 16 113 7 146 2.99e-25 97.4
Msa0416360 MtrunA17_Chr8g0359921 39.007 141 47 2 16 118 11 150 5.66e-25 96.3
Msa0416360 MtrunA17_Chr5g0405211 37.405 131 41 1 20 109 9 139 7.02e-25 96.3
Msa0416360 MtrunA17_Chr3g0130731 40.625 128 37 2 17 106 5 131 1.27e-24 94.7
Msa0416360 MtrunA17_Chr3g0128111 41.463 123 32 1 23 105 46 168 1.84e-24 93.6
Msa0416360 MtrunA17_Chr3g0128151 41.732 127 34 1 19 105 22 148 2.37e-24 96.3
Msa0416360 MtrunA17_Chr2g0318081 39.535 129 40 1 16 106 13 141 2.53e-24 95.1
Msa0416360 MtrunA17_Chr7g0214071 39.695 131 42 2 13 106 9 139 2.55e-24 93.2
Msa0416360 MtrunA17_Chr1g0183741 36.641 131 43 2 17 107 8 138 3.75e-24 93.2
Msa0416360 MtrunA17_Chr3g0124761 37.500 128 43 3 16 106 4 131 1.41e-23 92.0
Msa0416360 MtrunA17_Chr1g0200561 35.915 142 42 2 20 120 20 153 3.76e-23 91.7
Msa0416360 MtrunA17_Chr7g0263931 36.735 147 53 2 11 117 3 149 4.24e-23 91.3
Msa0416360 MtrunA17_Chr5g0417791 38.931 131 42 1 17 109 1 131 4.62e-23 90.1
Msa0416360 MtrunA17_Chr8g0384721 39.844 128 38 2 17 106 5 131 5.61e-23 90.5
Msa0416360 MtrunA17_Chr5g0428991 40.625 128 36 1 18 105 24 151 6.99e-23 92.4
Msa0416360 MtrunA17_Chr4g0042561 39.231 130 41 1 18 109 8 137 8.83e-23 89.7
Msa0416360 MtrunA17_Chr7g0261411 35.878 131 44 2 17 107 8 138 9.33e-23 89.7
Msa0416360 MtrunA17_Chr3g0130101 34.483 145 54 2 12 116 1 144 6.60e-22 88.6
Msa0416360 MtrunA17_Chr2g0318101 36.296 135 46 2 15 109 11 145 7.72e-22 88.2
Msa0416360 MtrunA17_Chr1g0197351 41.600 125 32 3 20 104 44 167 1.51e-21 88.2
Msa0416360 MtrunA17_Chr2g0311661 34.356 163 60 5 1 117 1 162 2.47e-21 86.3
Msa0416360 MtrunA17_Chr7g0252971 36.552 145 45 2 12 109 1 145 2.48e-21 86.7
Msa0416360 MtrunA17_Chr4g0047841 36.496 137 43 3 19 115 15 147 2.50e-21 86.7
Msa0416360 MtrunA17_Chr2g0326361 36.957 138 47 2 19 116 14 151 8.42e-21 85.1
Msa0416360 MtrunA17_Chr2g0311651 34.132 167 56 5 1 117 1 163 9.65e-21 84.7
Msa0416360 MtrunA17_Chr1g0197361 37.778 135 42 3 20 113 10 143 1.20e-20 85.9
Msa0416360 MtrunA17_Chr8g0364791 35.766 137 47 2 20 116 8 143 2.98e-20 84.7
Msa0416360 MtrunA17_Chr1g0201021 36.364 132 43 2 20 111 21 151 9.36e-20 83.2
Msa0416360 MtrunA17_Chr8g0385991 34.586 133 49 2 12 106 7 139 1.56e-19 80.1
Msa0416360 MtrunA17_Chr5g0400461 34.307 137 43 2 17 106 4 140 1.84e-19 81.6
Msa0416360 MtrunA17_Chr4g0054791 34.074 135 46 2 15 106 2 136 2.23e-19 81.3
Msa0416360 MtrunA17_Chr8g0384201 35.075 134 46 2 20 113 8 140 2.31e-19 82.0
Msa0416360 MtrunA17_Chr3g0092671 36.154 130 37 4 20 106 7 133 1.79e-17 76.6
Msa0416360 MtrunA17_Chr8g0345671 40.458 131 32 5 18 104 10 138 9.53e-17 74.7
Msa0416360 MtrunA17_Chr2g0321631 34.351 131 41 4 20 105 5 135 2.58e-16 72.0
Msa0416360 MtrunA17_Chr4g0038751 32.450 151 57 4 1 110 1 147 5.08e-16 72.0
Msa0416360 MtrunA17_Chr2g0321741 33.019 106 32 1 38 104 11 116 1.67e-13 65.5
Msa0416360 MtrunA17_Chr7g0243371 51.064 47 21 1 62 106 45 91 5.84e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0416360 AT5G61430.1 52.113 142 29 2 13 116 10 150 6.99e-41 138
Msa0416360 AT3G29035.1 51.389 144 32 1 3 108 8 151 1.52e-40 137
Msa0416360 AT5G07680.1 51.389 144 31 2 11 116 9 151 2.08e-40 137
Msa0416360 AT5G07680.2 52.174 138 27 2 17 116 1 137 4.29e-39 133
Msa0416360 AT5G53950.1 51.799 139 26 3 19 117 17 154 1.05e-37 131
Msa0416360 AT5G39610.1 51.493 134 27 1 13 108 14 147 2.44e-37 128
Msa0416360 AT3G18400.1 49.219 128 27 1 19 108 5 132 3.64e-37 128
Msa0416360 AT3G04060.1 48.322 149 37 3 7 116 8 155 1.05e-36 127
Msa0416360 AT5G18270.2 49.645 141 31 3 15 116 17 156 8.60e-36 125
Msa0416360 AT5G18270.1 49.645 141 31 3 15 116 17 156 9.26e-36 125
Msa0416360 AT2G24430.2 44.521 146 41 2 5 110 2 147 1.29e-33 119
Msa0416360 AT2G24430.1 44.521 146 41 2 5 110 2 147 1.29e-33 119
Msa0416360 AT3G15170.1 45.946 148 38 4 11 118 13 158 1.57e-33 119
Msa0416360 AT1G76420.1 44.366 142 38 3 13 114 17 157 7.78e-30 109
Msa0416360 AT3G12977.1 36.641 131 45 1 19 111 18 148 2.24e-27 102
Msa0416360 AT1G56010.2 34.459 148 58 2 1 109 1 148 6.68e-27 101
Msa0416360 AT2G46770.1 36.054 147 53 1 14 119 11 157 2.62e-26 100
Msa0416360 AT3G17730.1 39.695 131 39 1 17 107 4 134 3.95e-26 98.2
Msa0416360 AT4G36160.2 36.496 137 46 1 14 109 9 145 1.31e-25 99.4
Msa0416360 AT4G36160.1 36.496 137 46 1 14 109 5 141 1.47e-25 99.0
Msa0416360 AT4G36160.3 36.496 137 46 1 14 109 5 141 1.47e-25 99.0
Msa0416360 AT1G12260.2 39.535 129 37 1 20 107 8 136 1.70e-25 98.2
Msa0416360 AT1G65910.1 38.168 131 41 1 17 107 4 134 1.81e-25 100
Msa0416360 AT1G12260.1 39.535 129 37 1 20 107 8 136 2.57e-25 98.6
Msa0416360 AT4G10350.1 40.141 142 44 1 20 120 10 151 2.57e-25 97.8
Msa0416360 AT3G10500.1 41.732 127 33 2 20 106 11 136 5.85e-25 98.6
Msa0416360 AT3G03200.1 39.310 145 45 2 17 121 4 145 6.36e-25 98.2
Msa0416360 AT5G04410.1 41.667 132 36 2 20 111 11 141 8.75e-25 98.2
Msa0416360 AT2G18060.3 36.029 136 46 1 15 109 5 140 1.08e-24 96.7
Msa0416360 AT2G18060.1 36.029 136 46 1 15 109 5 140 1.08e-24 96.7
Msa0416360 AT1G33280.1 41.085 129 35 1 20 107 9 137 1.58e-24 95.1
Msa0416360 AT1G01720.1 38.931 131 41 2 14 106 2 131 1.94e-24 94.7
Msa0416360 AT2G27300.1 39.535 129 38 2 19 108 15 142 2.18e-24 95.1
Msa0416360 AT1G33060.1 42.520 127 33 1 19 105 24 150 2.72e-24 96.7
Msa0416360 AT1G33060.2 42.520 127 33 1 19 105 24 150 2.72e-24 96.7
Msa0416360 AT2G33480.1 40.000 125 40 1 17 106 13 137 2.74e-24 94.0
Msa0416360 AT4G35580.1 41.732 127 34 1 19 105 9 135 2.78e-24 96.7
Msa0416360 AT2G33480.2 40.000 125 40 1 17 106 13 137 3.00e-24 93.6
Msa0416360 AT3G10490.1 43.307 127 31 2 20 106 29 154 3.26e-24 93.2
Msa0416360 AT4G27410.2 38.760 129 41 1 16 106 11 139 3.28e-24 94.4
Msa0416360 AT3G15500.1 39.535 129 40 1 16 106 11 139 3.86e-24 94.4
Msa0416360 AT1G61110.1 36.735 147 54 2 5 112 2 148 6.15e-24 94.0
Msa0416360 AT4G35580.3 41.732 127 34 1 19 105 9 135 6.29e-24 95.5
Msa0416360 AT4G35580.2 41.732 127 34 1 19 105 9 135 6.30e-24 95.5
Msa0416360 AT3G10480.1 43.307 127 31 2 20 106 29 154 7.04e-24 95.1
Msa0416360 AT3G10480.2 43.307 127 31 2 20 106 29 154 7.07e-24 95.1
Msa0416360 AT1G71930.2 33.333 138 51 1 16 112 6 143 8.43e-24 93.6
Msa0416360 AT1G71930.1 33.333 138 51 1 16 112 6 143 8.43e-24 93.6
Msa0416360 AT1G52890.1 39.535 129 40 1 16 106 11 139 1.15e-23 93.2
Msa0416360 AT1G32770.1 36.429 140 48 1 14 112 11 150 1.24e-23 93.6
Msa0416360 AT5G62380.2 36.364 132 43 1 17 107 5 136 1.26e-23 93.6
Msa0416360 AT5G62380.1 36.364 132 43 1 17 107 5 136 1.26e-23 93.6
Msa0416360 AT3G61910.1 35.252 139 49 1 14 111 6 144 1.46e-23 93.2
Msa0416360 AT5G17260.1 39.310 145 45 2 17 121 4 145 2.28e-23 94.0
Msa0416360 AT5G08790.1 39.535 129 39 2 16 106 4 131 7.35e-23 90.5
Msa0416360 AT5G13180.1 35.606 132 48 2 12 106 7 138 1.35e-22 89.0
Msa0416360 AT1G62700.1 37.778 135 43 1 14 107 2 136 1.88e-22 90.9
Msa0416360 AT1G62700.2 37.778 135 43 1 14 107 2 136 1.88e-22 90.9
Msa0416360 AT5G66300.1 34.586 133 46 1 13 104 6 138 2.58e-22 89.0
Msa0416360 AT1G77450.1 37.500 128 41 2 17 106 8 134 2.94e-22 88.2
Msa0416360 AT3G10490.2 43.307 127 31 2 20 106 29 154 3.15e-22 90.5
Msa0416360 AT1G54330.1 40.000 130 38 1 17 106 4 133 4.21e-22 88.6
Msa0416360 AT3G04070.2 36.913 149 50 3 13 118 4 151 4.22e-22 89.4
Msa0416360 AT3G04070.1 36.913 149 50 3 13 118 4 151 5.80e-22 89.0
Msa0416360 AT1G26870.1 38.621 145 46 4 1 105 1 142 8.77e-22 89.0
Msa0416360 AT5G61430.2 70.909 55 15 1 62 116 34 87 2.26e-21 86.3
Msa0416360 AT1G79580.3 37.313 134 42 2 16 107 14 147 3.81e-21 87.0
Msa0416360 AT1G79580.5 37.313 134 42 2 16 107 14 147 3.81e-21 87.0
Msa0416360 AT1G79580.4 37.313 134 42 2 16 107 14 147 3.81e-21 87.0
Msa0416360 AT1G79580.2 37.313 134 42 2 16 107 14 147 3.81e-21 87.0
Msa0416360 AT1G79580.1 37.313 134 42 2 16 107 14 147 3.81e-21 87.0
Msa0416360 AT5G63790.2 38.060 134 43 3 11 106 53 184 4.15e-21 86.3
Msa0416360 AT5G63790.1 38.060 134 43 3 11 106 43 174 4.23e-21 86.3
Msa0416360 AT3G49530.1 35.714 154 52 4 4 115 2 150 9.84e-21 86.3
Msa0416360 AT5G22290.1 35.075 134 47 2 19 113 22 154 9.93e-21 85.5
Msa0416360 AT5G39820.1 35.811 148 54 3 8 115 10 156 1.42e-20 85.1
Msa0416360 AT2G43000.1 31.126 151 65 2 1 113 1 150 1.65e-20 84.0
Msa0416360 AT3G49530.2 35.714 154 52 4 4 115 2 150 1.90e-20 85.5
Msa0416360 AT5G46590.1 33.566 143 54 2 19 120 6 148 2.11e-20 84.0
Msa0416360 AT5G46590.2 34.559 136 48 2 19 113 6 141 2.69e-20 83.6
Msa0416360 AT4G27410.3 34.247 146 41 3 16 106 11 156 8.44e-20 82.8
Msa0416360 AT3G44290.2 36.364 132 44 2 19 111 15 145 1.81e-19 80.5
Msa0416360 AT4G28530.1 32.192 146 43 3 18 107 9 154 1.87e-19 82.0
Msa0416360 AT5G64060.1 33.577 137 50 2 20 116 8 143 2.33e-19 82.0
Msa0416360 AT4G17980.1 36.154 130 41 2 19 106 6 135 3.67e-19 80.1
Msa0416360 AT4G17980.2 36.154 130 41 2 19 106 6 135 5.59e-19 80.1
Msa0416360 AT5G09330.5 34.307 137 49 2 20 116 8 143 6.91e-19 81.3
Msa0416360 AT5G09330.2 34.307 137 49 2 20 116 8 143 6.91e-19 81.3
Msa0416360 AT5G09330.4 34.307 137 49 2 20 116 8 143 6.91e-19 81.3
Msa0416360 AT5G09330.3 34.307 137 49 2 20 116 8 143 6.91e-19 81.3
Msa0416360 AT5G09330.1 34.307 137 49 2 20 116 8 143 6.91e-19 81.3
Msa0416360 AT3G44290.1 36.364 132 44 2 19 111 15 145 7.04e-19 80.5
Msa0416360 AT5G24590.2 36.719 128 40 2 19 105 13 140 7.24e-19 81.3
Msa0416360 AT1G69490.1 36.508 126 42 1 19 106 9 134 7.78e-19 79.3
Msa0416360 AT1G32510.1 33.108 148 52 2 20 120 7 154 2.50e-18 78.2
Msa0416360 AT1G34190.1 36.029 136 46 2 16 111 14 148 4.24e-18 79.0
Msa0416360 AT2G17040.1 34.328 134 46 3 17 109 5 137 1.18e-17 76.3
Msa0416360 AT1G34180.1 36.029 136 46 2 16 111 14 148 1.42e-17 77.4
Msa0416360 AT1G34180.3 36.029 136 46 2 16 111 14 148 3.67e-17 76.3
Msa0416360 AT1G56010.1 60.417 48 19 0 62 109 34 81 2.34e-16 72.8
Msa0416360 AT3G12977.2 58.000 50 21 0 62 111 46 95 3.50e-16 71.6
Msa0416360 AT1G34180.2 33.108 148 46 2 16 111 14 160 5.69e-16 72.8
Msa0416360 AT2G18060.2 58.333 48 20 0 62 109 5 52 8.04e-16 71.6
Msa0416360 AT1G32870.1 38.400 125 38 2 20 106 12 135 8.36e-16 72.4
Msa0416360 AT1G52880.1 34.884 129 42 2 23 109 21 149 8.37e-16 72.0
Msa0416360 AT1G32870.2 38.400 125 38 2 20 106 12 135 8.51e-16 72.4
Msa0416360 AT1G32870.3 38.400 125 38 2 20 106 48 171 9.67e-16 72.4
Msa0416360 AT1G34180.4 33.108 148 46 2 16 111 14 160 1.49e-15 71.6
Msa0416360 AT3G10490.4 66.667 45 15 0 62 106 10 54 8.71e-15 69.3
Msa0416360 AT3G10490.3 66.667 45 15 0 62 106 10 54 8.71e-15 69.3
Msa0416360 AT3G10480.3 66.667 45 15 0 62 106 143 187 1.48e-14 68.9
Msa0416360 AT4G10350.2 54.237 59 27 0 62 120 46 104 4.44e-14 67.0
Msa0416360 AT2G33480.3 60.000 45 18 0 62 106 36 80 1.82e-13 64.3
Msa0416360 AT5G22290.2 48.077 52 27 0 62 113 4 55 2.85e-13 64.3

Find 24 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAGGTAAATGCATTAAT+TGG 0.103088 3_2:-63171218 Msa0416360:CDS
AAATGAAAAGGTCGAATTTC+CGG 0.216715 3_2:-63172400 Msa0416360:CDS
AATGAAAAGGTCGAATTTCC+GGG 0.351715 3_2:-63172399 Msa0416360:CDS
TCAAGGTAAATGCATTAATT+GGG 0.407011 3_2:-63171217 Msa0416360:CDS
CGAGTGGTGAAAAGACCAAT+TGG 0.426528 3_2:-63171157 Msa0416360:CDS
GTATTCATGCATGACCCAAT+TGG 0.496635 3_2:+63171142 None:intergenic
TTACCATTCCATGTGTGGAT+AGG 0.526862 3_2:+63171087 None:intergenic
ATGATTTATAAGCTCTTCAT+CGG 0.528076 3_2:+63172356 None:intergenic
TTTATAAGCTCTTCATCGGT+CGG 0.530344 3_2:+63172360 None:intergenic
AATTGGGATGAAGAAAACAT+TGG 0.537739 3_2:-63171201 Msa0416360:CDS
GAGTGGTGAAAAGACCAATT+GGG 0.539850 3_2:-63171156 Msa0416360:CDS
CAACCTATCCACACATGGAA+TGG 0.547037 3_2:-63171090 None:intergenic
AATTTACCTGGCAAATCCCA+TGG 0.560572 3_2:+63172258 None:intergenic
TGTGAACCATGGGATTTGCC+AGG 0.562377 3_2:-63172264 Msa0416360:intron
CAATATTACCATTCCATGTG+TGG 0.569497 3_2:+63171082 None:intergenic
GGTCATGCATGAATACCGAT+TGG 0.581760 3_2:-63171135 Msa0416360:CDS
AGAGTGTGTTACCCTCCAAT+CGG 0.588761 3_2:+63171120 None:intergenic
CATGCATGAATACCGATTGG+AGG 0.592763 3_2:-63171132 Msa0416360:CDS
ATGCATGAATACCGATTGGA+GGG 0.621001 3_2:-63171131 Msa0416360:CDS
TATGAATAAGTGTGAACCAT+GGG 0.641317 3_2:-63172274 Msa0416360:CDS
GAGCACAACCTATCCACACA+TGG 0.663138 3_2:-63171095 Msa0416360:CDS
ATATGAATAAGTGTGAACCA+TGG 0.681966 3_2:-63172275 Msa0416360:CDS
GGATAGAATCGAAAACAACC+CGG 0.686902 3_2:+63172381 None:intergenic
TACATAGGAAGAGCTCCGAG+TGG 0.745847 3_2:-63171173 Msa0416360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAACATTAAATTTACC+TGG + chr3_2:63171286-63171305 None:intergenic 15.0%
!!! CCATATTACTATTTTTTTTT+TGG + chr3_2:63171354-63171373 None:intergenic 15.0%
!! CCAAAAAAAAAATAGTAATA+TGG - chr3_2:63171351-63171370 Msa0416360:intron 15.0%
!! AAAAAGAAAGAAAAACAATT+TGG - chr3_2:63171781-63171800 Msa0416360:intron 15.0%
!!! AATTATGCTTTCTTATTTTT+TGG - chr3_2:63172148-63172167 Msa0416360:intron 15.0%
!! AAAAAAACATTAAATTTACC+TGG + chr3_2:63171286-63171305 None:intergenic 15.0%
!!! CCATATTACTATTTTTTTTT+TGG + chr3_2:63171354-63171373 None:intergenic 15.0%
!! CCAAAAAAAAAATAGTAATA+TGG - chr3_2:63171351-63171370 Msa0416360:intron 15.0%
!! AAAAAGAAAGAAAAACAATT+TGG - chr3_2:63171781-63171800 Msa0416360:intron 15.0%
!!! AATTATGCTTTCTTATTTTT+TGG - chr3_2:63172148-63172167 Msa0416360:intron 15.0%
!!! TTCATTTTCCATTTTTTGAT+TGG + chr3_2:63171116-63171135 None:intergenic 20.0%
!! TCAAAAAATGGAAAATGAAA+AGG - chr3_2:63171117-63171136 Msa0416360:CDS 20.0%
!! ATGAAAAAGAAGAAGAAAAA+TGG - chr3_2:63171859-63171878 Msa0416360:intron 20.0%
!!! TTTTTTGGTTTATTTAGAGA+TGG - chr3_2:63172163-63172182 Msa0416360:intron 20.0%
!!! TTCATTTTCCATTTTTTGAT+TGG + chr3_2:63171116-63171135 None:intergenic 20.0%
!! TCAAAAAATGGAAAATGAAA+AGG - chr3_2:63171117-63171136 Msa0416360:CDS 20.0%
!! ATGAAAAAGAAGAAGAAAAA+TGG - chr3_2:63171859-63171878 Msa0416360:intron 20.0%
!!! TTTTTTGGTTTATTTAGAGA+TGG - chr3_2:63172163-63172182 Msa0416360:intron 20.0%
! GAATGTTTCCAATCAAAAAA+TGG - chr3_2:63171105-63171124 Msa0416360:CDS 25.0%
! ATGATTTATAAGCTCTTCAT+CGG + chr3_2:63171176-63171195 None:intergenic 25.0%
! ACACATGAATATAGTCATAT+TGG + chr3_2:63171516-63171535 None:intergenic 25.0%
! CACATAAATTAAGTCTTCTT+TGG + chr3_2:63171740-63171759 None:intergenic 25.0%
! CAAAGAAGACTTAATTTATG+TGG - chr3_2:63171738-63171757 Msa0416360:intron 25.0%
! GAAAGAAAAACAATTTGGAA+TGG - chr3_2:63171786-63171805 Msa0416360:intron 25.0%
!! TGATAACGACAATATTTCTA+AGG + chr3_2:63171931-63171950 None:intergenic 25.0%
!!! TTTTAAAGTTATTACGAGTG+TGG - chr3_2:63172070-63172089 Msa0416360:intron 25.0%
!! AATGTATGTACTATGTCTTT+TGG - chr3_2:63172112-63172131 Msa0416360:intron 25.0%
! TTCAAGGTAAATGCATTAAT+TGG - chr3_2:63172311-63172330 Msa0416360:CDS 25.0%
! TCAAGGTAAATGCATTAATT+GGG - chr3_2:63172312-63172331 Msa0416360:CDS 25.0%
!!! AAAACATTGGTTTTCTACAT+AGG - chr3_2:63172341-63172360 Msa0416360:CDS 25.0%
! GAATGTTTCCAATCAAAAAA+TGG - chr3_2:63171105-63171124 Msa0416360:CDS 25.0%
! ATGATTTATAAGCTCTTCAT+CGG + chr3_2:63171176-63171195 None:intergenic 25.0%
! ACACATGAATATAGTCATAT+TGG + chr3_2:63171516-63171535 None:intergenic 25.0%
! CACATAAATTAAGTCTTCTT+TGG + chr3_2:63171740-63171759 None:intergenic 25.0%
! CAAAGAAGACTTAATTTATG+TGG - chr3_2:63171738-63171757 Msa0416360:intron 25.0%
! GAAAGAAAAACAATTTGGAA+TGG - chr3_2:63171786-63171805 Msa0416360:intron 25.0%
!! TGATAACGACAATATTTCTA+AGG + chr3_2:63171931-63171950 None:intergenic 25.0%
!!! TTTTAAAGTTATTACGAGTG+TGG - chr3_2:63172070-63172089 Msa0416360:intron 25.0%
!! AATGTATGTACTATGTCTTT+TGG - chr3_2:63172112-63172131 Msa0416360:intron 25.0%
! TTCAAGGTAAATGCATTAAT+TGG - chr3_2:63172311-63172330 Msa0416360:CDS 25.0%
! TCAAGGTAAATGCATTAATT+GGG - chr3_2:63172312-63172331 Msa0416360:CDS 25.0%
!!! AAAACATTGGTTTTCTACAT+AGG - chr3_2:63172341-63172360 Msa0416360:CDS 25.0%
AAATGAAAAGGTCGAATTTC+CGG - chr3_2:63171129-63171148 Msa0416360:CDS 30.0%
!!! TGTCATCAACTTTTTTGACA+AGG + chr3_2:63171201-63171220 None:intergenic 30.0%
ATATGAATAAGTGTGAACCA+TGG - chr3_2:63171254-63171273 Msa0416360:intron 30.0%
TATGAATAAGTGTGAACCAT+GGG - chr3_2:63171255-63171274 Msa0416360:intron 30.0%
! TTTTCGCAAATTCATCTGTT+TGG + chr3_2:63171470-63171489 None:intergenic 30.0%
ACAGATGAATTTGCGAAAAA+AGG - chr3_2:63171471-63171490 Msa0416360:intron 30.0%
CAGATGAATTTGCGAAAAAA+GGG - chr3_2:63171472-63171491 Msa0416360:intron 30.0%
AGATGAATTTGCGAAAAAAG+GGG - chr3_2:63171473-63171492 Msa0416360:intron 30.0%
CATCAATGTTACAGATATGT+AGG + chr3_2:63171553-63171572 None:intergenic 30.0%
! ACAATTTGGAATGGTGATTA+CGG - chr3_2:63171795-63171814 Msa0416360:intron 30.0%
! TACCAATGAAGGATGTTTTA+GGG + chr3_2:63171832-63171851 None:intergenic 30.0%
TCGTTATCATCTTACTTTGA+TGG - chr3_2:63171942-63171961 Msa0416360:intron 30.0%
TTATTTAGAGATGGCTAAGA+TGG - chr3_2:63172172-63172191 Msa0416360:intron 30.0%
TATTTAGAGATGGCTAAGAT+GGG - chr3_2:63172173-63172192 Msa0416360:intron 30.0%
!!! AATGGTATTTTTTCTGTGTG+AGG - chr3_2:63172204-63172223 Msa0416360:intron 30.0%
!!! ATGGTATTTTTTCTGTGTGA+GGG - chr3_2:63172205-63172224 Msa0416360:intron 30.0%
! CAAAGACAAAGAGATTTTCA+AGG - chr3_2:63172295-63172314 Msa0416360:CDS 30.0%
AATTGGGATGAAGAAAACAT+TGG - chr3_2:63172328-63172347 Msa0416360:CDS 30.0%
AAATGAAAAGGTCGAATTTC+CGG - chr3_2:63171129-63171148 Msa0416360:CDS 30.0%
!!! TGTCATCAACTTTTTTGACA+AGG + chr3_2:63171201-63171220 None:intergenic 30.0%
ATATGAATAAGTGTGAACCA+TGG - chr3_2:63171254-63171273 Msa0416360:intron 30.0%
TATGAATAAGTGTGAACCAT+GGG - chr3_2:63171255-63171274 Msa0416360:intron 30.0%
! TTTTCGCAAATTCATCTGTT+TGG + chr3_2:63171470-63171489 None:intergenic 30.0%
ACAGATGAATTTGCGAAAAA+AGG - chr3_2:63171471-63171490 Msa0416360:intron 30.0%
CAGATGAATTTGCGAAAAAA+GGG - chr3_2:63171472-63171491 Msa0416360:intron 30.0%
AGATGAATTTGCGAAAAAAG+GGG - chr3_2:63171473-63171492 Msa0416360:intron 30.0%
CATCAATGTTACAGATATGT+AGG + chr3_2:63171553-63171572 None:intergenic 30.0%
! ACAATTTGGAATGGTGATTA+CGG - chr3_2:63171795-63171814 Msa0416360:intron 30.0%
! TACCAATGAAGGATGTTTTA+GGG + chr3_2:63171832-63171851 None:intergenic 30.0%
TCGTTATCATCTTACTTTGA+TGG - chr3_2:63171942-63171961 Msa0416360:intron 30.0%
TTATTTAGAGATGGCTAAGA+TGG - chr3_2:63172172-63172191 Msa0416360:intron 30.0%
TATTTAGAGATGGCTAAGAT+GGG - chr3_2:63172173-63172192 Msa0416360:intron 30.0%
!!! AATGGTATTTTTTCTGTGTG+AGG - chr3_2:63172204-63172223 Msa0416360:intron 30.0%
!!! ATGGTATTTTTTCTGTGTGA+GGG - chr3_2:63172205-63172224 Msa0416360:intron 30.0%
! CAAAGACAAAGAGATTTTCA+AGG - chr3_2:63172295-63172314 Msa0416360:CDS 30.0%
AATTGGGATGAAGAAAACAT+TGG - chr3_2:63172328-63172347 Msa0416360:CDS 30.0%
AATGAAAAGGTCGAATTTCC+GGG - chr3_2:63171130-63171149 Msa0416360:CDS 35.0%
TTTATAAGCTCTTCATCGGT+CGG + chr3_2:63171172-63171191 None:intergenic 35.0%
CCAGATCTAATCAATGACTT+TGG + chr3_2:63171579-63171598 None:intergenic 35.0%
! CCAAAGTCATTGATTAGATC+TGG - chr3_2:63171576-63171595 Msa0416360:intron 35.0%
ATGCAGTGTTTATTTGACTC+AGG + chr3_2:63171626-63171645 None:intergenic 35.0%
AATGTCCTACTAAGACAAGA+CGG - chr3_2:63171696-63171715 Msa0416360:intron 35.0%
! CAATTTGGAATGGTGATTAC+GGG - chr3_2:63171796-63171815 Msa0416360:intron 35.0%
AACCCTAAAACATCCTTCAT+TGG - chr3_2:63171827-63171846 Msa0416360:intron 35.0%
! CTACCAATGAAGGATGTTTT+AGG + chr3_2:63171833-63171852 None:intergenic 35.0%
AAGAAAAATGGTGACCACTA+GGG - chr3_2:63171871-63171890 Msa0416360:intron 35.0%
CCTTACACATTATGATGTGT+CGG + chr3_2:63171999-63172018 None:intergenic 35.0%
AATGAAAAGGTCGAATTTCC+GGG - chr3_2:63171130-63171149 Msa0416360:CDS 35.0%
TTTATAAGCTCTTCATCGGT+CGG + chr3_2:63171172-63171191 None:intergenic 35.0%
CCAGATCTAATCAATGACTT+TGG + chr3_2:63171579-63171598 None:intergenic 35.0%
! CCAAAGTCATTGATTAGATC+TGG - chr3_2:63171576-63171595 Msa0416360:intron 35.0%
ATGCAGTGTTTATTTGACTC+AGG + chr3_2:63171626-63171645 None:intergenic 35.0%
AATGTCCTACTAAGACAAGA+CGG - chr3_2:63171696-63171715 Msa0416360:intron 35.0%
! CAATTTGGAATGGTGATTAC+GGG - chr3_2:63171796-63171815 Msa0416360:intron 35.0%
AACCCTAAAACATCCTTCAT+TGG - chr3_2:63171827-63171846 Msa0416360:intron 35.0%
! CTACCAATGAAGGATGTTTT+AGG + chr3_2:63171833-63171852 None:intergenic 35.0%
AAGAAAAATGGTGACCACTA+GGG - chr3_2:63171871-63171890 Msa0416360:intron 35.0%
CCTTACACATTATGATGTGT+CGG + chr3_2:63171999-63172018 None:intergenic 35.0%
GGATAGAATCGAAAACAACC+CGG + chr3_2:63171151-63171170 None:intergenic 40.0%
AATTTACCTGGCAAATCCCA+TGG + chr3_2:63171274-63171293 None:intergenic 40.0%
TTTCACCGTCTTGTCTTAGT+AGG + chr3_2:63171704-63171723 None:intergenic 40.0%
ACATCCTTCATTGGTAGATC+TGG - chr3_2:63171836-63171855 Msa0416360:intron 40.0%
TCATCCAGATCTACCAATGA+AGG + chr3_2:63171843-63171862 None:intergenic 40.0%
GAAGAAAAATGGTGACCACT+AGG - chr3_2:63171870-63171889 Msa0416360:intron 40.0%
CCGACACATCATAATGTGTA+AGG - chr3_2:63171996-63172015 Msa0416360:intron 40.0%
! CACATCTGATAGTGTCATGT+TGG + chr3_2:63172030-63172049 None:intergenic 40.0%
CAACATGACACTATCAGATG+TGG - chr3_2:63172028-63172047 Msa0416360:intron 40.0%
GAAAATACCCAACTGGTCAA+AGG - chr3_2:63172231-63172250 Msa0416360:intron 40.0%
TATTGGTCCTTTGACCAGTT+GGG + chr3_2:63172241-63172260 None:intergenic 40.0%
ACCAATAGAGCTACTAATGC+TGG - chr3_2:63172254-63172273 Msa0416360:intron 40.0%
! TCCAGCATTAGTAGCTCTAT+TGG + chr3_2:63172258-63172277 None:intergenic 40.0%
GAGCTACTAATGCTGGATAT+TGG - chr3_2:63172261-63172280 Msa0416360:intron 40.0%
!!! CAATTGGTCTTTTCACCACT+CGG + chr3_2:63172374-63172393 None:intergenic 40.0%
GAGTGGTGAAAAGACCAATT+GGG - chr3_2:63172373-63172392 Msa0416360:CDS 40.0%
GTATTCATGCATGACCCAAT+TGG + chr3_2:63172390-63172409 None:intergenic 40.0%
! ATGCATGAATACCGATTGGA+GGG - chr3_2:63172398-63172417 Msa0416360:CDS 40.0%
GGATAGAATCGAAAACAACC+CGG + chr3_2:63171151-63171170 None:intergenic 40.0%
AATTTACCTGGCAAATCCCA+TGG + chr3_2:63171274-63171293 None:intergenic 40.0%
TTTCACCGTCTTGTCTTAGT+AGG + chr3_2:63171704-63171723 None:intergenic 40.0%
ACATCCTTCATTGGTAGATC+TGG - chr3_2:63171836-63171855 Msa0416360:intron 40.0%
TCATCCAGATCTACCAATGA+AGG + chr3_2:63171843-63171862 None:intergenic 40.0%
GAAGAAAAATGGTGACCACT+AGG - chr3_2:63171870-63171889 Msa0416360:intron 40.0%
CCGACACATCATAATGTGTA+AGG - chr3_2:63171996-63172015 Msa0416360:intron 40.0%
! CACATCTGATAGTGTCATGT+TGG + chr3_2:63172030-63172049 None:intergenic 40.0%
CAACATGACACTATCAGATG+TGG - chr3_2:63172028-63172047 Msa0416360:intron 40.0%
GAAAATACCCAACTGGTCAA+AGG - chr3_2:63172231-63172250 Msa0416360:intron 40.0%
TATTGGTCCTTTGACCAGTT+GGG + chr3_2:63172241-63172260 None:intergenic 40.0%
ACCAATAGAGCTACTAATGC+TGG - chr3_2:63172254-63172273 Msa0416360:intron 40.0%
! TCCAGCATTAGTAGCTCTAT+TGG + chr3_2:63172258-63172277 None:intergenic 40.0%
GAGCTACTAATGCTGGATAT+TGG - chr3_2:63172261-63172280 Msa0416360:intron 40.0%
!!! CAATTGGTCTTTTCACCACT+CGG + chr3_2:63172374-63172393 None:intergenic 40.0%
GAGTGGTGAAAAGACCAATT+GGG - chr3_2:63172373-63172392 Msa0416360:CDS 40.0%
GTATTCATGCATGACCCAAT+TGG + chr3_2:63172390-63172409 None:intergenic 40.0%
! ATGCATGAATACCGATTGGA+GGG - chr3_2:63172398-63172417 Msa0416360:CDS 40.0%
AAATGGTGACCACTAGGGTT+TGG - chr3_2:63171876-63171895 Msa0416360:intron 45.0%
CTAAGATGGGAGAAGCAGAA+TGG - chr3_2:63172186-63172205 Msa0416360:intron 45.0%
AGGGAGAGAAAATACCCAAC+TGG - chr3_2:63172224-63172243 Msa0416360:intron 45.0%
CTATTGGTCCTTTGACCAGT+TGG + chr3_2:63172242-63172261 None:intergenic 45.0%
GCTGGATATTGGAAAGCAAC+AGG - chr3_2:63172272-63172291 Msa0416360:CDS 45.0%
CGAGTGGTGAAAAGACCAAT+TGG - chr3_2:63172372-63172391 Msa0416360:CDS 45.0%
GGTCATGCATGAATACCGAT+TGG - chr3_2:63172394-63172413 Msa0416360:CDS 45.0%
! CATGCATGAATACCGATTGG+AGG - chr3_2:63172397-63172416 Msa0416360:CDS 45.0%
AGAGTGTGTTACCCTCCAAT+CGG + chr3_2:63172412-63172431 None:intergenic 45.0%
AAATGGTGACCACTAGGGTT+TGG - chr3_2:63171876-63171895 Msa0416360:intron 45.0%
CTAAGATGGGAGAAGCAGAA+TGG - chr3_2:63172186-63172205 Msa0416360:intron 45.0%
AGGGAGAGAAAATACCCAAC+TGG - chr3_2:63172224-63172243 Msa0416360:intron 45.0%
CTATTGGTCCTTTGACCAGT+TGG + chr3_2:63172242-63172261 None:intergenic 45.0%
GCTGGATATTGGAAAGCAAC+AGG - chr3_2:63172272-63172291 Msa0416360:CDS 45.0%
CGAGTGGTGAAAAGACCAAT+TGG - chr3_2:63172372-63172391 Msa0416360:CDS 45.0%
GGTCATGCATGAATACCGAT+TGG - chr3_2:63172394-63172413 Msa0416360:CDS 45.0%
! CATGCATGAATACCGATTGG+AGG - chr3_2:63172397-63172416 Msa0416360:CDS 45.0%
AGAGTGTGTTACCCTCCAAT+CGG + chr3_2:63172412-63172431 None:intergenic 45.0%
TGTGAACCATGGGATTTGCC+AGG - chr3_2:63171265-63171284 Msa0416360:intron 50.0%
TTCTCCTTGCCAAACCCTAG+TGG + chr3_2:63171888-63171907 None:intergenic 50.0%
TTGGCAAGGAGAAAAGCGAG+TGG - chr3_2:63171895-63171914 Msa0416360:intron 50.0%
TACATAGGAAGAGCTCCGAG+TGG - chr3_2:63172356-63172375 Msa0416360:CDS 50.0%
GAGCACAACCTATCCACACA+TGG - chr3_2:63172434-63172453 Msa0416360:CDS 50.0%
TGTGAACCATGGGATTTGCC+AGG - chr3_2:63171265-63171284 Msa0416360:intron 50.0%
TTCTCCTTGCCAAACCCTAG+TGG + chr3_2:63171888-63171907 None:intergenic 50.0%
TTGGCAAGGAGAAAAGCGAG+TGG - chr3_2:63171895-63171914 Msa0416360:intron 50.0%
TACATAGGAAGAGCTCCGAG+TGG - chr3_2:63172356-63172375 Msa0416360:CDS 50.0%
GAGCACAACCTATCCACACA+TGG - chr3_2:63172434-63172453 Msa0416360:CDS 50.0%
GTGACCACTAGGGTTTGGCA+AGG - chr3_2:63171881-63171900 Msa0416360:intron 55.0%
GTGACCACTAGGGTTTGGCA+AGG - chr3_2:63171881-63171900 Msa0416360:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 63171091 63172460 63171091 ID=Msa0416360;Name=Msa0416360
chr3_2 mRNA 63171091 63172460 63171091 ID=Msa0416360-mRNA-1;Parent=Msa0416360;Name=Msa0416360-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|1|1|2|0|121
chr3_2 exon 63172265 63172460 63172265 ID=Msa0416360-mRNA-1:exon:13710;Parent=Msa0416360-mRNA-1
chr3_2 exon 63171091 63171257 63171091 ID=Msa0416360-mRNA-1:exon:13709;Parent=Msa0416360-mRNA-1
chr3_2 CDS 63172265 63172460 63172265 ID=Msa0416360-mRNA-1:cds;Parent=Msa0416360-mRNA-1
chr3_2 CDS 63171091 63171257 63171091 ID=Msa0416360-mRNA-1:cds;Parent=Msa0416360-mRNA-1
Gene Sequence

>Msa0416360

ATGGGATCAAATGAGAATGTTTCCAATCAAAAAATGGAAAATGAAAAGGTCGAATTTCCGGGTTGTTTTCGATTCTATCCGACCGATGAAGAGCTTATAAATCATTACCTTGTCAAAAAAGTTGATGACAATAGCTTCTCTGCTATAGCTATTGCTGAAGTTGATATGAATAAGTGTGAACCATGGGATTTGCCAGGCAAAGACAAAGAGATTTTCAAGGTAAATGCATTAATTGGGATGAAGAAAACATTGGTTTTCTACATAGGAAGAGCTCCGAGTGGTGAAAAGACCAATTGGGTCATGCATGAATACCGATTGGAGGGTAACACACTCTCTGAGCACAACCTATCCACACATGGAATG
Protein sequence

>Msa0416360

MGSNENVSNQKMENEKVEFPGCFRFYPTDEELINHYLVKKVDDNSFSAIAIAEVDMNKCEPWDLPGKDKEIFKVNALIGMKKTLVFYIGRAPSGEKTNWVMHEYRLEGNTLSEHNLSTHGM