AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0421110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0421110 MtrunA17_Chr3g0121041 92.574 202 7 1 4 197 2 203 1.21e-135 377
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0421110 AT2G04845.1 63.819 199 64 1 7 197 3 201 4.27e-88 258
Msa0421110 AT2G04845.5 66.092 174 51 1 7 172 3 176 1.50e-83 246
Msa0421110 AT2G04845.2 66.250 160 46 1 7 158 3 162 6.78e-77 229
Msa0421110 AT2G04845.3 66.250 160 46 1 7 158 3 162 8.28e-77 229
Msa0421110 AT2G04845.4 66.250 160 46 1 7 158 3 162 8.94e-77 229

Find 60 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCTTAACAAGGATAAAA+TGG 0.271503 3_2:+70005164 None:intergenic
TGCATCCATTCATGATATTT+CGG 0.285663 3_2:+70009127 None:intergenic
TTCGATGGCGGCGGCGGCGG+CGG 0.296755 3_2:-70009221 Msa0421110:exon
AAAGGAAATGTGAGTTTAGA+AGG 0.318086 3_2:-70009190 Msa0421110:CDS
GAGTTCGATGGCGGCGGCGG+CGG 0.327667 3_2:-70009224 Msa0421110:exon
GCCTTTGCTATTGAGAAGTT+GGG 0.345000 3_2:-70007829 Msa0421110:CDS
CAAGGAAACTTTCATTGTCT+TGG 0.360883 3_2:-70009014 Msa0421110:CDS
CTTGTTGGAACTTTCTCTCA+TGG 0.385982 3_2:-70008980 Msa0421110:CDS
CATAGCCGTGGCAAAGGACT+TGG 0.395075 3_2:-70007877 Msa0421110:CDS
GCGGAGTTCGATGGCGGCGG+CGG 0.403087 3_2:-70009227 Msa0421110:exon
TGAGGAGATGCTTGGTTTGA+TGG 0.406506 3_2:-70005396 Msa0421110:CDS
GGAACGTGAGGTTCCATGTA+AGG 0.421077 3_2:+70009148 None:intergenic
CTTCTGCAGCTTATGCGTTA+CGG 0.428156 3_2:+70009296 None:intergenic
GCGGCGGCGGCGAGGAAGAA+AGG 0.437378 3_2:-70009208 Msa0421110:CDS
GGCCTTTGCTATTGAGAAGT+TGG 0.437922 3_2:-70007830 Msa0421110:CDS
ATACTCTTGTTCGAGAGAAA+GGG 0.455235 3_2:+70009069 None:intergenic
GATACTCTTGTTCGAGAGAA+AGG 0.456194 3_2:+70009068 None:intergenic
GCTGCGCTTTGCTCTTGCCA+CGG 0.465838 3_2:+70009251 None:intergenic
ACCAAGCATCTCCTCACTCT+TGG 0.466967 3_2:+70005403 None:intergenic
GCGGCGGAGTTCGATGGCGG+CGG 0.470509 3_2:-70009230 Msa0421110:exon
ATTTGTTTGACAGGTGACTT+TGG 0.470985 3_2:-70005444 Msa0421110:intron
TCACAATCTTGTACTGGTAA+TGG 0.473597 3_2:+70005268 None:intergenic
GTCAAATTCTTACACTTGAA+AGG 0.486297 3_2:+70005222 None:intergenic
ACGTTCCGAAATATCATGAA+TGG 0.490153 3_2:-70009132 Msa0421110:CDS
CATGGTCAACCCCACGTTGA+AGG 0.491566 3_2:-70008962 Msa0421110:intron
GGTAATACTGGTCCCTTACA+TGG 0.495614 3_2:-70009161 Msa0421110:CDS
AGCGCAGCGGCGGAGTTCGA+TGG 0.496615 3_2:-70009236 Msa0421110:exon
ACATTATTGACCTTCAACGT+GGG 0.501922 3_2:+70008952 None:intergenic
TTGTTCGAGAGAAAGGGGTT+CGG 0.511970 3_2:+70009075 None:intergenic
TGTGAGTTTAGAAGGAAAGA+AGG 0.512492 3_2:-70009182 Msa0421110:CDS
AGAAGGAAAGAAGGTAATAC+TGG 0.516666 3_2:-70009173 Msa0421110:CDS
ATAATGTCACAATCTTGTAC+TGG 0.540859 3_2:+70005262 None:intergenic
GCAGTGGCTTGAAGTAGAGA+AGG 0.544829 3_2:+70009100 None:intergenic
GCAAGAGCAAAGCGCAGCGG+CGG 0.545002 3_2:-70009246 Msa0421110:five_prime_UTR
ATTTCAACCTCTGCCACTTG+AGG 0.545729 3_2:+70008796 None:intergenic
GCCAAGAGTGAGGAGATGCT+TGG 0.546877 3_2:-70005404 Msa0421110:CDS
TGTTAGCATAGCCGTGGCAA+AGG 0.550626 3_2:-70007883 Msa0421110:intron
CCGCTATTTGCAGCTATGGT+TGG 0.553290 3_2:-70008853 Msa0421110:intron
CAATAATCCTCAAGTGGCAG+AGG 0.554435 3_2:-70008803 Msa0421110:intron
GAGGAGATGCTTGGTTTGAT+GGG 0.563113 3_2:-70005395 Msa0421110:CDS
GCAGCGGCGGAGTTCGATGG+CGG 0.564183 3_2:-70009233 Msa0421110:exon
TCATGATATTTCGGAACGTG+AGG 0.572117 3_2:+70009136 None:intergenic
CCTTTGCTATTGAGAAGTTG+GGG 0.572445 3_2:-70007828 Msa0421110:CDS
AGTATCAGATGCAACTCTCA+TGG 0.583647 3_2:-70009051 Msa0421110:CDS
AGAAAGGGGTTCGGAAGCAG+TGG 0.587837 3_2:+70009084 None:intergenic
CCTTTCCAAGTCCTTTGCCA+CGG 0.588992 3_2:+70007872 None:intergenic
AACATTATTGACCTTCAACG+TGG 0.591929 3_2:+70008951 None:intergenic
TACTCTTGTTCGAGAGAAAG+GGG 0.600981 3_2:+70009070 None:intergenic
CCGTGGCAAAGGACTTGGAA+AGG 0.603951 3_2:-70007872 Msa0421110:CDS
CGTGTGAACCCATCTTAACA+AGG 0.606372 3_2:+70005155 None:intergenic
CCCCAACTTCTCAATAGCAA+AGG 0.607006 3_2:+70007828 None:intergenic
TGATATCAATAATCCTCAAG+TGG 0.607377 3_2:-70008809 Msa0421110:CDS
GAACGTGAGGTTCCATGTAA+GGG 0.624781 3_2:+70009149 None:intergenic
AAAATCAATTGGTCAGTCCG+TGG 0.631608 3_2:-70009268 Msa0421110:five_prime_UTR
CATTATTGACCTTCAACGTG+GGG 0.634827 3_2:+70008953 None:intergenic
CAGTGGCTTGAAGTAGAGAA+GGG 0.645058 3_2:+70009101 None:intergenic
AATAACACAAGCCAAGAGTG+AGG 0.663808 3_2:-70005414 Msa0421110:CDS
AAATACGCACATCATGAACA+AGG 0.665884 3_2:+70005303 None:intergenic
GTGGCAAGAGCAAAGCGCAG+CGG 0.684523 3_2:-70009249 Msa0421110:five_prime_UTR
ATGGTCACGTGATCTCAACA+AGG 0.702832 3_2:-70009032 Msa0421110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAATAAAAATGACAAAA+TGG + chr3_2:70005830-70005849 None:intergenic 15.0%
!! ATCGATTAAAAATTATACAT+AGG + chr3_2:70006003-70006022 None:intergenic 15.0%
!!! TTTTAAATTTAGAACATAGA+TGG + chr3_2:70006417-70006436 None:intergenic 15.0%
!!! AAAACTTTTCAAGTTAAAAT+TGG - chr3_2:70006656-70006675 Msa0421110:intron 15.0%
!! TAATATGAGAAAGAAAAAAA+AGG + chr3_2:70006743-70006762 None:intergenic 15.0%
!!! TTTTTTCTTTCTCATATTAA+AGG - chr3_2:70006744-70006763 Msa0421110:intron 15.0%
!! TATTCTAGATTATCTATTTA+GGG + chr3_2:70006795-70006814 None:intergenic 15.0%
!! ATATTCTAGATTATCTATTT+AGG + chr3_2:70006796-70006815 None:intergenic 15.0%
!! TTTAAGAAATATGATAGAAT+AGG - chr3_2:70007671-70007690 Msa0421110:intron 15.0%
!!! TAATTTCAAATTTTCAATAG+AGG - chr3_2:70008349-70008368 Msa0421110:intron 15.0%
!!! TGTTATTGTAATTTTAACTA+CGG - chr3_2:70008466-70008485 Msa0421110:intron 15.0%
!! CAGAAGAAAAAAAAATCAAT+TGG - chr3_2:70005179-70005198 Msa0421110:three_prime_UTR 20.0%
!! TATGAGATGAATTTATAAGT+AGG + chr3_2:70005712-70005731 None:intergenic 20.0%
!! GATGAAAATTACTTTAATAC+AGG - chr3_2:70006077-70006096 Msa0421110:intron 20.0%
!! ATACTCTACTTGAATTATAA+AGG + chr3_2:70006103-70006122 None:intergenic 20.0%
!! TCTGAAAAATAGAAATTGAA+TGG - chr3_2:70007020-70007039 Msa0421110:intron 20.0%
!! CTGAAAAATAGAAATTGAAT+GGG - chr3_2:70007021-70007040 Msa0421110:intron 20.0%
!! TGAAAAATAGAAATTGAATG+GGG - chr3_2:70007022-70007041 Msa0421110:intron 20.0%
!! TATAAATGGTAAACTTAATC+TGG - chr3_2:70007231-70007250 Msa0421110:intron 20.0%
!! TTTATTACGAAGAATATTCT+TGG - chr3_2:70007536-70007555 Msa0421110:intron 20.0%
!!! ATGAAGTTACAGTTTTTTAT+TGG - chr3_2:70008065-70008084 Msa0421110:intron 20.0%
!!! AAAATATTCTTTTAGAACCA+TGG - chr3_2:70008145-70008164 Msa0421110:intron 20.0%
!! GATTAAGGTAAATTAAAATC+TGG - chr3_2:70008582-70008601 Msa0421110:intron 20.0%
!!! TTTCCTTTAATCAAACTTTT+CGG - chr3_2:70008614-70008633 Msa0421110:intron 20.0%
!! ATGGTTAAAACAAAAACTTA+GGG + chr3_2:70008838-70008857 None:intergenic 20.0%
!! AATGGTTAAAACAAAAACTT+AGG + chr3_2:70008839-70008858 None:intergenic 20.0%
!!! GTTTATTATGTTCTAAGTAA+TGG + chr3_2:70008857-70008876 None:intergenic 20.0%
!! TTGGATAAAGATTTTCTTGT+TGG - chr3_2:70005463-70005482 Msa0421110:intron 25.0%
!!! AAATTAGCTTTAATTGGACA+TGG + chr3_2:70005954-70005973 None:intergenic 25.0%
! ATGTCCAATTAAAGCTAATT+TGG - chr3_2:70005953-70005972 Msa0421110:intron 25.0%
!!! AACACCAAATTAGCTTTAAT+TGG + chr3_2:70005960-70005979 None:intergenic 25.0%
! ATATCCCAGATAAAAATTAG+AGG - chr3_2:70006033-70006052 Msa0421110:intron 25.0%
!! AAATTAGAGGCGTAAAAAAT+AGG - chr3_2:70006046-70006065 Msa0421110:intron 25.0%
!!! TGTCATCTCTAAAACATAAA+AGG - chr3_2:70006177-70006196 Msa0421110:intron 25.0%
!!! ATCTAGAACTCTTATGTTTT+GGG - chr3_2:70006486-70006505 Msa0421110:intron 25.0%
! TTTCTCATATTAAAGGAATG+AGG - chr3_2:70006751-70006770 Msa0421110:intron 25.0%
! CTTTGTCTTATGTATTAAGA+CGG + chr3_2:70006886-70006905 None:intergenic 25.0%
! TTTCAGATAAACAGATAGAA+AGG + chr3_2:70007007-70007026 None:intergenic 25.0%
! TTACTTCTTAATTTAATCGC+TGG - chr3_2:70007276-70007295 Msa0421110:intron 25.0%
! TATAAATAGGAGAACACAAA+GGG + chr3_2:70007778-70007797 None:intergenic 25.0%
!!! ACAGTGCTATTTGTATAAAT+AGG + chr3_2:70007791-70007810 None:intergenic 25.0%
! TAAGAGTTGTTAATTAGTTG+AGG + chr3_2:70007823-70007842 None:intergenic 25.0%
!! GAACTTAGGATTTAGAATAA+TGG + chr3_2:70007869-70007888 None:intergenic 25.0%
!! TCTACTTGTTAAATTCTCTT+TGG - chr3_2:70007920-70007939 Msa0421110:intron 25.0%
!!! AAAGCCTTTAAAATGAAAGA+GGG + chr3_2:70008021-70008040 None:intergenic 25.0%
! AATCAATTCTATAAAAGCCA+TGG + chr3_2:70008165-70008184 None:intergenic 25.0%
! AAACCGAAAAGTTTGATTAA+AGG + chr3_2:70008620-70008639 None:intergenic 25.0%
!! AAACTTTTCGGTTTATCTTA+TGG - chr3_2:70008626-70008645 Msa0421110:intron 25.0%
! AAGAAGAAAACCACATATAA+CGG + chr3_2:70008955-70008974 None:intergenic 25.0%
!! CATTTTATCCTTGTTAAGAT+GGG - chr3_2:70009295-70009314 Msa0421110:five_prime_UTR 25.0%
AAAGGAAATGTGAGTTTAGA+AGG - chr3_2:70005268-70005287 Msa0421110:three_prime_UTR 30.0%
TGCATCCATTCATGATATTT+CGG + chr3_2:70005334-70005353 None:intergenic 30.0%
TGATATCAATAATCCTCAAG+TGG - chr3_2:70005649-70005668 Msa0421110:intron 30.0%
TGAATTTATAAGTAGGTACC+TGG + chr3_2:70005705-70005724 None:intergenic 30.0%
! TACGCCTCTAATTTTTATCT+GGG + chr3_2:70006040-70006059 None:intergenic 30.0%
! TTACGCCTCTAATTTTTATC+TGG + chr3_2:70006041-70006060 None:intergenic 30.0%
!! GCATATGTATGCTTTTTAAC+TGG - chr3_2:70006258-70006277 Msa0421110:intron 30.0%
!! CATCTAGAACTCTTATGTTT+TGG - chr3_2:70006485-70006504 Msa0421110:intron 30.0%
CGTCTTAATACATAAGACAA+AGG - chr3_2:70006884-70006903 Msa0421110:intron 30.0%
! AAGACAAAGGTACATTATAC+AGG - chr3_2:70006897-70006916 Msa0421110:intron 30.0%
! AGACAAAGGTACATTATACA+GGG - chr3_2:70006898-70006917 Msa0421110:intron 30.0%
ATACAGGGAAATACATGAAT+TGG - chr3_2:70006913-70006932 Msa0421110:intron 30.0%
TGTAGCATACACATTAAACA+AGG + chr3_2:70007125-70007144 None:intergenic 30.0%
TTAATGTGTATGCTACACTA+AGG - chr3_2:70007128-70007147 Msa0421110:intron 30.0%
!! GAACTTGGCTTTTATATCTA+AGG + chr3_2:70007190-70007209 None:intergenic 30.0%
GTCAAGGTTATTATAGAACT+TGG + chr3_2:70007205-70007224 None:intergenic 30.0%
ACCTTGACACACATTATAAA+TGG - chr3_2:70007217-70007236 Msa0421110:intron 30.0%
ACCATTTATAATGTGTGTCA+AGG + chr3_2:70007221-70007240 None:intergenic 30.0%
TGAAAATCTTGTGCATCAAA+TGG + chr3_2:70007357-70007376 None:intergenic 30.0%
ATTGATCAATTCAATGACCT+TGG + chr3_2:70007469-70007488 None:intergenic 30.0%
AATCAATTCATATGAAGCCA+TGG + chr3_2:70007564-70007583 None:intergenic 30.0%
GTATAAATAGGAGAACACAA+AGG + chr3_2:70007779-70007798 None:intergenic 30.0%
!! CAAAGCCTTTAAAATGAAAG+AGG + chr3_2:70008022-70008041 None:intergenic 30.0%
!! CTCTTTCATTTTAAAGGCTT+TGG - chr3_2:70008020-70008039 Msa0421110:intron 30.0%
! ACATAGCAACATTTTCAAAG+AGG - chr3_2:70008310-70008329 Msa0421110:intron 30.0%
AGTTACAAGCTAAGTGATTA+AGG - chr3_2:70008567-70008586 Msa0421110:intron 30.0%
TAACAGTTTGTGAAATCAAC+AGG + chr3_2:70008679-70008698 None:intergenic 30.0%
!!! TGTTTTTTAGCCGTTATATG+TGG - chr3_2:70008942-70008961 Msa0421110:intron 30.0%
!! TTGTTCCTAATTTGTTTGAC+AGG - chr3_2:70009005-70009024 Msa0421110:CDS 30.0%
ATAATGTCACAATCTTGTAC+TGG + chr3_2:70009199-70009218 None:intergenic 30.0%
GTCAAATTCTTACACTTGAA+AGG + chr3_2:70009239-70009258 None:intergenic 30.0%
CCATCTTAACAAGGATAAAA+TGG + chr3_2:70009297-70009316 None:intergenic 30.0%
! CCATTTTATCCTTGTTAAGA+TGG - chr3_2:70009294-70009313 Msa0421110:five_prime_UTR 30.0%
TGTGAGTTTAGAAGGAAAGA+AGG - chr3_2:70005276-70005295 Msa0421110:three_prime_UTR 35.0%
AGAAGGAAAGAAGGTAATAC+TGG - chr3_2:70005285-70005304 Msa0421110:three_prime_UTR 35.0%
ACGTTCCGAAATATCATGAA+TGG - chr3_2:70005326-70005345 Msa0421110:three_prime_UTR 35.0%
ATACTCTTGTTCGAGAGAAA+GGG + chr3_2:70005392-70005411 None:intergenic 35.0%
CAAGGAAACTTTCATTGTCT+TGG - chr3_2:70005444-70005463 Msa0421110:intron 35.0%
ACATTATTGACCTTCAACGT+GGG + chr3_2:70005509-70005528 None:intergenic 35.0%
AACATTATTGACCTTCAACG+TGG + chr3_2:70005510-70005529 None:intergenic 35.0%
TTATGATAGCTGAACAAACC+AGG - chr3_2:70005684-70005703 Msa0421110:intron 35.0%
ACGCCTTCAATAAGAAATGT+TGG + chr3_2:70005759-70005778 None:intergenic 35.0%
! GTTTTCCAAAACTTTGTCCA+TGG + chr3_2:70006366-70006385 None:intergenic 35.0%
!! AGAGTTCTAGATGAAAGACA+CGG + chr3_2:70006478-70006497 None:intergenic 35.0%
AGTGATCAAAAGTGAGATCA+AGG + chr3_2:70006546-70006565 None:intergenic 35.0%
!!! TTTTTTTGTTAGCATAGCCG+TGG - chr3_2:70006569-70006588 Msa0421110:intron 35.0%
!!! GGAGTCTGTTTTGATAATGA+TGG - chr3_2:70006607-70006626 Msa0421110:intron 35.0%
!!! CAGTTTTTTGAACAGGTTGA+TGG + chr3_2:70006699-70006718 None:intergenic 35.0%
CATCAACCTGTTCAAAAAAC+TGG - chr3_2:70006697-70006716 Msa0421110:intron 35.0%
AAAAAAGGATAGAGAAGCAG+AGG + chr3_2:70006728-70006747 None:intergenic 35.0%
CACTTCTGATACTAGATAAC+TGG - chr3_2:70006828-70006847 Msa0421110:intron 35.0%
TGGAATAGAGTCGATATACA+AGG - chr3_2:70006848-70006867 Msa0421110:intron 35.0%
AGCAGCTATGATTATGCTTA+TGG + chr3_2:70007082-70007101 None:intergenic 35.0%
ACAAGGAAACATGTGCATAA+CGG + chr3_2:70007108-70007127 None:intergenic 35.0%
ATGGTAAACTTAATCTGGTG+AGG - chr3_2:70007236-70007255 Msa0421110:intron 35.0%
GAAGTAATGCTCATTTACCA+TGG + chr3_2:70007263-70007282 None:intergenic 35.0%
TCAGTATTGCATGTAGTTCA+AGG - chr3_2:70007374-70007393 Msa0421110:intron 35.0%
TAACACAGTGTTCATTTCGA+AGG - chr3_2:70007436-70007455 Msa0421110:intron 35.0%
GAAGAATATTCTTGGAACCA+TGG - chr3_2:70007544-70007563 Msa0421110:intron 35.0%
TTCCATTCCATTCCATTCTT+GGG + chr3_2:70007635-70007654 None:intergenic 35.0%
ATTCCATTCCATTCCATTCT+TGG + chr3_2:70007636-70007655 None:intergenic 35.0%
! AGAGCATTCTCTATTGTGAA+GGG + chr3_2:70007722-70007741 None:intergenic 35.0%
! TAGAGCATTCTCTATTGTGA+AGG + chr3_2:70007723-70007742 None:intergenic 35.0%
GAGTTGTTAATTAGTTGAGG+TGG + chr3_2:70007820-70007839 None:intergenic 35.0%
! CTGTCCCTCTTTCATTTTAA+AGG - chr3_2:70008014-70008033 Msa0421110:intron 35.0%
! AATCCCAGTCTCAATTGTTT+TGG - chr3_2:70008509-70008528 Msa0421110:intron 35.0%
TAGCCAAAACAATTGAGACT+GGG + chr3_2:70008515-70008534 None:intergenic 35.0%
ATAGCCAAAACAATTGAGAC+TGG + chr3_2:70008516-70008535 None:intergenic 35.0%
AGTCACCTGTCAAACAAATT+AGG + chr3_2:70009013-70009032 None:intergenic 35.0%
ATTTGTTTGACAGGTGACTT+TGG - chr3_2:70009014-70009033 Msa0421110:CDS 35.0%
AAATACGCACATCATGAACA+AGG + chr3_2:70009158-70009177 None:intergenic 35.0%
! TCACAATCTTGTACTGGTAA+TGG + chr3_2:70009193-70009212 None:intergenic 35.0%
AAAATCAATTGGTCAGTCCG+TGG - chr3_2:70005190-70005209 Msa0421110:three_prime_UTR 40.0%
TCATGATATTTCGGAACGTG+AGG + chr3_2:70005325-70005344 None:intergenic 40.0%
TACTCTTGTTCGAGAGAAAG+GGG + chr3_2:70005391-70005410 None:intergenic 40.0%
GATACTCTTGTTCGAGAGAA+AGG + chr3_2:70005393-70005412 None:intergenic 40.0%
AGTATCAGATGCAACTCTCA+TGG - chr3_2:70005407-70005426 Msa0421110:CDS 40.0%
CTTGTTGGAACTTTCTCTCA+TGG - chr3_2:70005478-70005497 Msa0421110:intron 40.0%
CATTATTGACCTTCAACGTG+GGG + chr3_2:70005508-70005527 None:intergenic 40.0%
GCGCCAACATTTCTTATTGA+AGG - chr3_2:70005753-70005772 Msa0421110:intron 40.0%
! TTTCTTATTGAAGGCGTGTC+TGG - chr3_2:70005762-70005781 Msa0421110:intron 40.0%
TAACACAGACACATTGACAC+TGG + chr3_2:70005859-70005878 None:intergenic 40.0%
GTCATGTTTGTAACCGATTG+AGG - chr3_2:70005894-70005913 Msa0421110:intron 40.0%
ATAAACCATTCCACAGACCA+TGG - chr3_2:70006346-70006365 Msa0421110:intron 40.0%
AAAACTTTGTCCATGGTCTG+TGG + chr3_2:70006359-70006378 None:intergenic 40.0%
! ACAGACCATGGACAAAGTTT+TGG - chr3_2:70006358-70006377 Msa0421110:intron 40.0%
!! AGTTCTAGATGAAAGACACG+GGG + chr3_2:70006476-70006495 None:intergenic 40.0%
!! GAGTTCTAGATGAAAGACAC+GGG + chr3_2:70006477-70006496 None:intergenic 40.0%
!! GCCTTTGCTATTGAGAAGTT+GGG - chr3_2:70006629-70006648 Msa0421110:intron 40.0%
!! CCTTTGCTATTGAGAAGTTG+GGG - chr3_2:70006630-70006649 Msa0421110:intron 40.0%
!!! GACAGACCAGTTTTTTGAAC+AGG + chr3_2:70006706-70006725 None:intergenic 40.0%
AGGTTCAACTTGCACTTACT+TGG - chr3_2:70007148-70007167 Msa0421110:intron 40.0%
! GAAGTGTCTTTCTCTGTCTT+TGG - chr3_2:70007603-70007622 Msa0421110:intron 40.0%
TGTCTTTCTCTGTCTTTGGT+AGG - chr3_2:70007607-70007626 Msa0421110:intron 40.0%
AACCCAAGAATGGAATGGAA+TGG - chr3_2:70007630-70007649 Msa0421110:intron 40.0%
TATTGTGAAGGGGAAACTCT+TGG + chr3_2:70007711-70007730 None:intergenic 40.0%
GAGCATTCTCTATTGTGAAG+GGG + chr3_2:70007721-70007740 None:intergenic 40.0%
CAAGAGACTGTTAGGAACTT+AGG + chr3_2:70007883-70007902 None:intergenic 40.0%
ACTTGAAGCAAGAGACTGTT+AGG + chr3_2:70007891-70007910 None:intergenic 40.0%
TAACAGTCTCTTGCTTCAAG+TGG - chr3_2:70007890-70007909 Msa0421110:intron 40.0%
! TCTTTGGTTGACTTAGCTTG+CGG - chr3_2:70007936-70007955 Msa0421110:intron 40.0%
AAACCACTTGAGAAGAGAGA+GGG + chr3_2:70008224-70008243 None:intergenic 40.0%
AATCCACAAGCTAAGTCAAC+AGG + chr3_2:70008265-70008284 None:intergenic 40.0%
!! TTAGCTTGTGGATTTGATGC+AGG - chr3_2:70008271-70008290 Msa0421110:intron 40.0%
! TAGCTTGTGGATTTGATGCA+GGG - chr3_2:70008272-70008291 Msa0421110:intron 40.0%
AATAACACAAGCCAAGAGTG+AGG - chr3_2:70009044-70009063 Msa0421110:CDS 40.0%
CTTCTGCAGCTTATGCGTTA+CGG + chr3_2:70005165-70005184 None:intergenic 45.0%
GGTAATACTGGTCCCTTACA+TGG - chr3_2:70005297-70005316 Msa0421110:three_prime_UTR 45.0%
GAACGTGAGGTTCCATGTAA+GGG + chr3_2:70005312-70005331 None:intergenic 45.0%
CAGTGGCTTGAAGTAGAGAA+GGG + chr3_2:70005360-70005379 None:intergenic 45.0%
TTGTTCGAGAGAAAGGGGTT+CGG + chr3_2:70005386-70005405 None:intergenic 45.0%
ATGGTCACGTGATCTCAACA+AGG - chr3_2:70005426-70005445 Msa0421110:CDS 45.0%
CCAACCATAGCTGCAAATAG+CGG + chr3_2:70005608-70005627 None:intergenic 45.0%
CAATAATCCTCAAGTGGCAG+AGG - chr3_2:70005655-70005674 Msa0421110:intron 45.0%
! ATTTCAACCTCTGCCACTTG+AGG + chr3_2:70005665-70005684 None:intergenic 45.0%
TTTGTCCATGGTCTGTGGAA+TGG + chr3_2:70006354-70006373 None:intergenic 45.0%
!! GGCCTTTGCTATTGAGAAGT+TGG - chr3_2:70006628-70006647 Msa0421110:intron 45.0%
CCCCAACTTCTCAATAGCAA+AGG + chr3_2:70006633-70006652 None:intergenic 45.0%
ACTTAATCTGGTGAGGACCA+TGG - chr3_2:70007243-70007262 Msa0421110:intron 45.0%
CTTTGGTAGGAACCCAAGAA+TGG - chr3_2:70007620-70007639 Msa0421110:intron 45.0%
GTAGGAACCCAAGAATGGAA+TGG - chr3_2:70007625-70007644 Msa0421110:intron 45.0%
ATAGGAGAACACAAAGGGCA+GGG + chr3_2:70007773-70007792 None:intergenic 45.0%
AATAGGAGAACACAAAGGGC+AGG + chr3_2:70007774-70007793 None:intergenic 45.0%
! TAGCTTGCGGATTGATGTAG+AGG - chr3_2:70007949-70007968 Msa0421110:intron 45.0%
GAAACCACTTGAGAAGAGAG+AGG + chr3_2:70008225-70008244 None:intergenic 45.0%
!! TCTCCTGTTGACTTAGCTTG+TGG - chr3_2:70008259-70008278 Msa0421110:intron 45.0%
AGCTTGTGGATTTGATGCAG+GGG - chr3_2:70008273-70008292 Msa0421110:intron 45.0%
GACGCGCATTCAATAAAGCA+CGG + chr3_2:70008715-70008734 None:intergenic 45.0%
! TGAGGAGATGCTTGGTTTGA+TGG - chr3_2:70009062-70009081 Msa0421110:CDS 45.0%
! GAGGAGATGCTTGGTTTGAT+GGG - chr3_2:70009063-70009082 Msa0421110:CDS 45.0%
CGTGTGAACCCATCTTAACA+AGG + chr3_2:70009306-70009325 None:intergenic 45.0%
GGAACGTGAGGTTCCATGTA+AGG + chr3_2:70005313-70005332 None:intergenic 50.0%
GCAGTGGCTTGAAGTAGAGA+AGG + chr3_2:70005361-70005380 None:intergenic 50.0%
! TGTGCCGCTATTTGCAGCTA+TGG - chr3_2:70005601-70005620 Msa0421110:intron 50.0%
CCGCTATTTGCAGCTATGGT+TGG - chr3_2:70005605-70005624 Msa0421110:intron 50.0%
! ATTGAAGGCGTGTCTGGTGT+CGG - chr3_2:70005768-70005787 Msa0421110:intron 50.0%
CCAACACGTGCATCCTCAAT+CGG + chr3_2:70005910-70005929 None:intergenic 50.0%
CGATTGAGGATGCACGTGTT+GGG - chr3_2:70005908-70005927 Msa0421110:intron 50.0%
GATTGAGGATGCACGTGTTG+GGG - chr3_2:70005909-70005928 Msa0421110:intron 50.0%
TGTTAGCATAGCCGTGGCAA+AGG - chr3_2:70006575-70006594 Msa0421110:intron 50.0%
CCTTTCCAAGTCCTTTGCCA+CGG + chr3_2:70006589-70006608 None:intergenic 50.0%
ATTTCGAAGGCTGCAGTCCA+AGG - chr3_2:70007449-70007468 Msa0421110:intron 50.0%
TGTGAAGGGGAAACTCTTGG+AGG + chr3_2:70007708-70007727 None:intergenic 50.0%
TCTCCCTCTCTCTTCTCAAG+TGG - chr3_2:70008218-70008237 Msa0421110:intron 50.0%
ACCAAGCATCTCCTCACTCT+TGG + chr3_2:70009058-70009077 None:intergenic 50.0%
AGAAAGGGGTTCGGAAGCAG+TGG + chr3_2:70005377-70005396 None:intergenic 55.0%
CATGGTCAACCCCACGTTGA+AGG - chr3_2:70005496-70005515 Msa0421110:intron 55.0%
! TGGTGTCGGACATGTGACAC+TGG - chr3_2:70005782-70005801 Msa0421110:intron 55.0%
TGTCGGACATGTGACACTGG+AGG - chr3_2:70005785-70005804 Msa0421110:intron 55.0%
CCGATTGAGGATGCACGTGT+TGG - chr3_2:70005907-70005926 Msa0421110:intron 55.0%
CATAGCCGTGGCAAAGGACT+TGG - chr3_2:70006581-70006600 Msa0421110:intron 55.0%
CCGTGGCAAAGGACTTGGAA+AGG - chr3_2:70006586-70006605 Msa0421110:intron 55.0%
GCCAAGAGTGAGGAGATGCT+TGG - chr3_2:70009054-70009073 Msa0421110:CDS 55.0%
GCTGCGCTTTGCTCTTGCCA+CGG + chr3_2:70005210-70005229 None:intergenic 60.0%
GTGGCAAGAGCAAAGCGCAG+CGG - chr3_2:70005209-70005228 Msa0421110:three_prime_UTR 60.0%
GCAAGAGCAAAGCGCAGCGG+CGG - chr3_2:70005212-70005231 Msa0421110:three_prime_UTR 65.0%
AGCGCAGCGGCGGAGTTCGA+TGG - chr3_2:70005222-70005241 Msa0421110:three_prime_UTR 70.0%
GCAGCGGCGGAGTTCGATGG+CGG - chr3_2:70005225-70005244 Msa0421110:three_prime_UTR 70.0%
! GCGGCGGAGTTCGATGGCGG+CGG - chr3_2:70005228-70005247 Msa0421110:three_prime_UTR 75.0%
! GCGGAGTTCGATGGCGGCGG+CGG - chr3_2:70005231-70005250 Msa0421110:three_prime_UTR 75.0%
! GAGTTCGATGGCGGCGGCGG+CGG - chr3_2:70005234-70005253 Msa0421110:three_prime_UTR 75.0%
GCGGCGGCGGCGAGGAAGAA+AGG - chr3_2:70005250-70005269 Msa0421110:three_prime_UTR 75.0%
!! TTCGATGGCGGCGGCGGCGG+CGG - chr3_2:70005237-70005256 Msa0421110:three_prime_UTR 80.0%
! TGGCGGCGGCGGCGGCGGCG+AGG - chr3_2:70005242-70005261 Msa0421110:three_prime_UTR 95.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 70005148 70009332 70005148 ID=Msa0421110;Name=Msa0421110
chr3_2 mRNA 70005148 70009332 70005148 ID=Msa0421110-mRNA-1;Parent=Msa0421110;Name=Msa0421110-mRNA-1;_AED=0.06;_eAED=0.06;_QI=93|0.75|0.6|1|1|1|5|225|197
chr3_2 exon 70005148 70005453 70005148 ID=Msa0421110-mRNA-1:exon:16743;Parent=Msa0421110-mRNA-1
chr3_2 exon 70006149 70006187 70006149 ID=Msa0421110-mRNA-1:exon:16742;Parent=Msa0421110-mRNA-1
chr3_2 exon 70007762 70007899 70007762 ID=Msa0421110-mRNA-1:exon:16741;Parent=Msa0421110-mRNA-1
chr3_2 exon 70008804 70008862 70008804 ID=Msa0421110-mRNA-1:exon:16740;Parent=Msa0421110-mRNA-1
chr3_2 exon 70008963 70009332 70008963 ID=Msa0421110-mRNA-1:exon:16739;Parent=Msa0421110-mRNA-1
chr3_2 five_prime_UTR 70009240 70009332 70009240 ID=Msa0421110-mRNA-1:five_prime_utr;Parent=Msa0421110-mRNA-1
chr3_2 CDS 70008963 70009239 70008963 ID=Msa0421110-mRNA-1:cds;Parent=Msa0421110-mRNA-1
chr3_2 CDS 70008804 70008862 70008804 ID=Msa0421110-mRNA-1:cds;Parent=Msa0421110-mRNA-1
chr3_2 CDS 70007762 70007899 70007762 ID=Msa0421110-mRNA-1:cds;Parent=Msa0421110-mRNA-1
chr3_2 CDS 70006149 70006187 70006149 ID=Msa0421110-mRNA-1:cds;Parent=Msa0421110-mRNA-1
chr3_2 CDS 70005373 70005453 70005373 ID=Msa0421110-mRNA-1:cds;Parent=Msa0421110-mRNA-1
chr3_2 three_prime_UTR 70005148 70005372 70005148 ID=Msa0421110-mRNA-1:three_prime_utr;Parent=Msa0421110-mRNA-1
Gene Sequence

>Msa0421110

ATGGCGGCGGCGGCGGCGGCGAGGAAGAAAGGAAATGTGAGTTTAGAAGGAAAGAAGGTAATACTGGTCCCTTACATGGAACCTCACGTTCCGAAATATCATGAATGGATGCAAGACCCTTCTCTACTTCAAGCCACTGCTTCCGAACCCCTTTCTCTCGAACAAGAGTATCAGATGCAACTCTCATGGTCACGTGATCTCAACAAGGAAACTTTCATTGTCTTGGATAAAGATTTTCTTGTTGGAACTTTCTCTCATGGTCAACCCCACGTTGAAGCTATGGTTGGTGATGTAAATATTTTCATGAATGATATCAATAATCCTCAAGTGGCAGAGCATAGCCGTGGCAAAGGACTTGGAAAGGAGTCTGTTTTGATAATGATGGCCTTTGCTATTGAGAAGTTGGGGATCAAAACTTTTCAAGTTAAAATTGGAGAATCAAATGTAGAGTCCATCAACCTGTTCAAAAAACTGGGGTTTGTGCAAACTTCACATAGCAACATTTTCAAAGAGGTGACTTTGGAGTTGCAAATAACACAAGCCAAGAGTGAGGAGATGCTTGGTTTGATGGGTACTGTGATTAAACATACATAA
Protein sequence

>Msa0421110

MAAAAAARKKGNVSLEGKKVILVPYMEPHVPKYHEWMQDPSLLQATASEPLSLEQEYQMQLSWSRDLNKETFIVLDKDFLVGTFSHGQPHVEAMVGDVNIFMNDINNPQVAEHSRGKGLGKESVLIMMAFAIEKLGIKTFQVKIGESNVESINLFKKLGFVQTSHSNIFKEVTLELQITQAKSEEMLGLMGTVIKHT*