AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0426070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0426070 MtrunA17_Chr3g0128111 92.405 158 12 0 9 166 89 246 4.90e-107 305
Msa0426070 MtrunA17_Chr3g0128151 80.488 164 25 1 9 165 69 232 6.36e-89 271
Msa0426070 MtrunA17_Chr5g0428991 67.073 164 47 1 9 165 72 235 2.80e-74 233
Msa0426070 MtrunA17_Chr3g0109821 49.655 145 68 3 11 152 55 197 4.79e-38 137
Msa0426070 MtrunA17_Chr6g0481091 42.690 171 82 2 7 165 51 217 4.92e-38 130
Msa0426070 MtrunA17_Chr5g0433781 50.350 143 63 3 11 153 55 189 5.61e-38 137
Msa0426070 MtrunA17_Chr3g0113001 43.671 158 78 3 8 154 3 160 7.37e-37 128
Msa0426070 MtrunA17_Chr2g0309891 44.966 149 62 5 11 153 55 189 4.27e-35 125
Msa0426070 MtrunA17_Chr3g0092671 57.143 105 44 1 9 113 53 156 6.31e-35 127
Msa0426070 MtrunA17_Chr5g0398971 53.535 99 44 2 15 112 61 158 7.23e-34 122
Msa0426070 MtrunA17_Chr4g0018391 48.000 125 43 3 8 112 34 156 5.70e-33 119
Msa0426070 MtrunA17_Chr5g0400461 44.371 151 65 5 11 149 55 198 8.54e-33 119
Msa0426070 MtrunA17_Chr2g0321741 50.000 110 43 2 17 121 46 148 2.79e-32 118
Msa0426070 MtrunA17_Chr8g0372121 48.980 98 48 1 15 112 60 155 2.99e-32 117
Msa0426070 MtrunA17_Chr1g0197361 55.769 104 45 1 10 113 57 159 5.78e-32 119
Msa0426070 MtrunA17_Chr8g0364791 48.077 104 54 0 9 112 53 156 6.69e-32 118
Msa0426070 MtrunA17_Chr7g0217711 63.636 77 28 0 12 88 67 143 1.57e-31 111
Msa0426070 MtrunA17_Chr1g0197351 50.877 114 50 1 7 114 87 200 1.95e-31 117
Msa0426070 MtrunA17_Chr3g0091921 42.500 160 71 4 14 154 66 223 2.09e-31 114
Msa0426070 MtrunA17_Chr6g0484441 53.608 97 43 1 17 113 58 152 3.01e-31 114
Msa0426070 MtrunA17_Chr1g0200561 51.579 95 44 1 18 112 76 168 3.81e-31 115
Msa0426070 MtrunA17_Chr4g0056541 46.729 107 55 1 15 121 61 165 3.88e-31 115
Msa0426070 MtrunA17_Chr5g0405211 50.000 98 47 1 15 112 62 157 3.91e-31 115
Msa0426070 MtrunA17_Chr7g0217721 38.235 170 89 2 12 165 67 236 7.89e-31 114
Msa0426070 MtrunA17_Chr3g0139331 56.436 101 40 2 14 112 61 159 9.70e-31 114
Msa0426070 MtrunA17_Chr4g0060931 54.545 99 43 1 17 113 70 168 1.12e-30 114
Msa0426070 MtrunA17_Chr3g0130101 48.039 102 53 0 11 112 56 157 1.19e-30 113
Msa0426070 MtrunA17_Chr4g0017921 51.020 98 43 2 18 114 65 158 2.89e-30 112
Msa0426070 MtrunA17_Chr6g0453451 50.926 108 44 2 19 124 75 175 2.97e-30 112
Msa0426070 MtrunA17_Chr1g0201021 50.485 103 51 0 11 113 68 170 8.22e-30 114
Msa0426070 MtrunA17_Chr4g0054791 50.877 114 51 3 11 120 55 167 8.70e-30 110
Msa0426070 MtrunA17_Chr3g0113031 53.922 102 36 3 27 124 3 97 1.51e-29 108
Msa0426070 MtrunA17_Chr2g0318081 50.000 102 47 2 17 116 69 168 3.84e-29 109
Msa0426070 MtrunA17_Chr8g0359921 50.485 103 47 2 17 116 67 168 7.71e-29 108
Msa0426070 MtrunA17_Chr2g0309331 48.958 96 46 2 18 112 67 160 1.36e-28 108
Msa0426070 MtrunA17_Chr4g0042561 49.515 103 44 2 16 112 61 161 1.65e-28 106
Msa0426070 MtrunA17_Chr8g0384201 42.453 106 61 0 7 112 51 156 2.49e-28 109
Msa0426070 MtrunA17_Chr1g0147421 54.167 96 40 2 19 112 83 176 6.47e-28 106
Msa0426070 MtrunA17_Chr2g0312321 42.647 136 59 4 19 140 64 194 6.57e-28 105
Msa0426070 MtrunA17_Chr8g0346001 39.474 152 62 4 8 129 42 193 7.77e-28 107
Msa0426070 MtrunA17_Chr2g0316971 50.000 110 41 3 14 114 73 177 1.17e-27 106
Msa0426070 MtrunA17_Chr8g0345691 51.456 103 42 3 10 110 61 157 3.92e-27 105
Msa0426070 MtrunA17_Chr8g0345671 54.455 101 38 4 11 109 61 155 8.38e-27 103
Msa0426070 MtrunA17_Chr8g0361731 45.045 111 50 3 19 118 69 179 9.17e-27 103
Msa0426070 MtrunA17_Chr6g0461661 49.020 102 48 2 17 114 71 172 1.13e-26 103
Msa0426070 MtrunA17_Chr7g0214071 49.000 100 47 2 15 112 63 160 1.17e-26 101
Msa0426070 MtrunA17_Chr7g0252971 38.776 147 74 3 17 147 61 207 1.45e-26 102
Msa0426070 MtrunA17_Chr5g0417791 44.538 119 60 2 17 129 56 174 1.60e-26 100
Msa0426070 MtrunA17_Chr7g0253011 50.485 103 45 2 17 113 70 172 2.26e-26 102
Msa0426070 MtrunA17_Chr1g0183741 46.602 103 49 2 12 112 58 156 3.72e-26 100
Msa0426070 MtrunA17_Chr5g0417001 43.750 112 53 3 17 118 67 178 5.84e-26 101
Msa0426070 MtrunA17_Chr2g0318101 43.243 111 50 2 17 114 68 178 7.41e-26 100
Msa0426070 MtrunA17_Chr8g0384721 48.039 102 44 3 17 112 60 158 8.55e-26 99.8
Msa0426070 MtrunA17_Chr3g0124761 46.535 101 46 3 17 112 60 157 1.11e-25 99.4
Msa0426070 MtrunA17_Chr4g0076731 48.454 97 47 2 17 112 107 201 6.15e-25 99.4
Msa0426070 MtrunA17_Chr3g0130731 40.602 133 52 5 3 112 29 157 8.19e-25 97.1
Msa0426070 MtrunA17_Chr7g0261411 46.078 102 49 2 12 111 58 155 9.04e-25 97.1
Msa0426070 MtrunA17_Chr4g0038751 46.465 99 45 2 17 112 68 161 1.56e-24 96.7
Msa0426070 MtrunA17_Chr4g0047841 41.463 123 47 3 19 116 70 192 2.65e-24 96.7
Msa0426070 MtrunA17_Chr7g0263931 38.406 138 68 3 17 137 64 201 2.88e-24 96.3
Msa0426070 MtrunA17_Chr8g0385991 43.750 112 52 3 16 118 64 173 4.46e-24 94.0
Msa0426070 MtrunA17_Chr3g0144271 55.696 79 33 1 12 88 72 150 4.61e-24 97.1
Msa0426070 MtrunA17_Chr6g0453131 43.396 106 50 2 19 114 70 175 2.78e-23 94.4
Msa0426070 MtrunA17_Chr2g0326361 40.476 126 58 4 17 132 67 185 5.95e-22 90.1
Msa0426070 MtrunA17_Chr2g0321631 44.118 102 51 5 14 110 60 160 7.37e-21 85.9
Msa0426070 MtrunA17_Chr7g0243371 43.333 90 49 1 1 88 1 90 1.61e-20 81.6
Msa0426070 MtrunA17_Chr7g0266701 39.423 104 57 3 16 113 62 165 2.07e-20 84.7
Msa0426070 MtrunA17_Chr8g0345711 47.191 89 44 2 11 97 62 149 3.11e-20 82.4
Msa0426070 MtrunA17_Chr8g0345741 38.938 113 61 3 11 121 41 147 5.12e-20 81.3
Msa0426070 MtrunA17_Chr8g0345721 47.500 80 39 2 11 88 114 192 3.98e-19 80.5
Msa0426070 MtrunA17_Chr3g0131911 39.815 108 55 2 21 118 69 176 6.48e-19 80.5
Msa0426070 MtrunA17_Chr4g0026141 38.835 103 59 2 12 110 58 160 1.38e-18 80.1
Msa0426070 MtrunA17_Chr8g0345731 47.561 82 39 2 11 88 74 155 3.60e-18 77.0
Msa0426070 MtrunA17_Chr8g0345701 47.368 95 31 5 10 88 56 147 3.29e-17 74.7
Msa0426070 MtrunA17_Chr5g0442771 41.667 108 51 4 17 112 71 178 2.48e-16 74.7
Msa0426070 MtrunA17_Chr2g0311651 39.286 112 53 3 17 113 76 187 8.61e-16 73.2
Msa0426070 MtrunA17_Chr2g0311661 38.393 112 54 3 17 113 75 186 6.04e-15 70.5
Msa0426070 MtrunA17_Chr4g0050701 36.782 87 47 2 37 115 76 162 1.10e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0426070 AT4G35580.1 60.274 146 55 1 10 152 57 202 1.54e-54 180
Msa0426070 AT4G35580.3 60.274 146 55 1 10 152 57 202 1.87e-54 180
Msa0426070 AT4G35580.2 60.274 146 55 1 10 152 57 202 1.99e-54 180
Msa0426070 AT1G33060.2 65.854 123 42 0 10 132 72 194 7.17e-54 180
Msa0426070 AT1G33060.1 65.854 123 42 0 10 132 72 194 7.57e-54 180
Msa0426070 AT5G24590.2 53.595 153 60 4 9 160 60 202 1.82e-46 157
Msa0426070 AT3G49530.2 53.472 144 61 2 9 151 60 198 1.05e-45 154
Msa0426070 AT3G49530.1 53.472 144 61 2 9 151 60 198 1.24e-45 155
Msa0426070 AT1G65910.1 52.101 119 57 0 11 129 55 173 3.45e-38 137
Msa0426070 AT3G17730.1 49.254 134 63 1 7 135 51 184 3.77e-38 130
Msa0426070 AT3G10490.1 56.604 106 45 1 10 114 75 180 6.57e-37 127
Msa0426070 AT3G10490.2 56.364 110 44 2 9 114 71 180 6.19e-36 129
Msa0426070 AT3G10480.2 56.190 105 46 0 10 114 75 179 8.07e-36 129
Msa0426070 AT3G10500.1 55.238 105 47 0 9 113 56 160 9.20e-36 130
Msa0426070 AT3G10480.1 57.407 108 41 2 10 114 75 180 1.08e-35 129
Msa0426070 AT3G10480.3 54.237 118 48 3 1 114 98 213 2.20e-35 129
Msa0426070 AT5G04410.1 54.286 105 48 0 9 113 56 160 1.13e-34 127
Msa0426070 AT5G17260.1 58.252 103 42 1 11 112 55 157 2.38e-34 125
Msa0426070 AT3G03200.1 56.311 103 44 1 11 112 55 157 2.09e-33 123
Msa0426070 AT4G17980.1 40.437 183 79 7 11 165 55 235 1.03e-32 117
Msa0426070 AT1G62700.1 54.082 98 43 2 15 112 61 156 1.51e-32 119
Msa0426070 AT1G62700.2 54.082 98 43 2 15 112 61 156 1.51e-32 119
Msa0426070 AT4G17980.2 40.437 183 79 7 11 165 55 235 2.57e-32 117
Msa0426070 AT2G18060.3 44.800 125 47 2 8 112 36 158 7.23e-32 117
Msa0426070 AT2G18060.1 44.800 125 47 2 8 112 36 158 7.23e-32 117
Msa0426070 AT1G12260.2 43.151 146 73 5 15 160 61 196 8.52e-32 117
Msa0426070 AT3G12977.2 50.000 102 50 1 19 119 20 121 1.01e-31 114
Msa0426070 AT1G34180.4 49.107 112 57 0 2 113 68 179 1.05e-31 118
Msa0426070 AT5G62380.2 48.113 106 53 1 16 121 62 165 1.10e-31 116
Msa0426070 AT5G62380.1 48.113 106 53 1 16 121 62 165 1.10e-31 116
Msa0426070 AT3G18400.1 55.102 98 41 2 17 113 58 153 1.19e-31 115
Msa0426070 AT1G34180.3 51.923 104 50 0 10 113 64 167 1.37e-31 118
Msa0426070 AT1G12260.1 53.061 98 44 2 15 112 61 156 1.54e-31 117
Msa0426070 AT5G46590.1 53.704 108 48 2 9 114 53 160 1.93e-31 114
Msa0426070 AT5G61430.2 51.923 104 48 1 12 113 1 104 1.97e-31 114
Msa0426070 AT1G34180.2 49.107 112 57 0 2 113 68 179 2.12e-31 118
Msa0426070 AT1G34180.1 51.923 104 50 0 10 113 64 167 3.01e-31 118
Msa0426070 AT4G10350.2 48.454 97 50 0 18 114 19 115 3.32e-31 114
Msa0426070 AT3G44290.2 52.336 107 42 3 9 115 61 158 4.08e-31 112
Msa0426070 AT1G71930.2 50.000 98 47 1 15 112 63 158 6.23e-31 114
Msa0426070 AT1G71930.1 50.000 98 47 1 15 112 63 158 6.23e-31 114
Msa0426070 AT4G36160.1 43.200 125 49 2 8 112 37 159 6.64e-31 115
Msa0426070 AT4G36160.3 43.200 125 49 2 8 112 37 159 6.64e-31 115
Msa0426070 AT5G61430.1 51.923 104 48 1 12 113 64 167 7.22e-31 114
Msa0426070 AT4G36160.2 43.200 125 49 2 8 112 41 163 7.49e-31 115
Msa0426070 AT4G10350.1 48.454 97 50 0 18 114 66 162 8.45e-31 114
Msa0426070 AT1G54330.1 53.398 103 45 1 11 113 55 154 9.26e-31 113
Msa0426070 AT5G39610.1 51.429 105 49 1 17 119 73 177 1.10e-30 112
Msa0426070 AT3G12977.1 49.038 104 52 1 19 121 73 176 1.14e-30 112
Msa0426070 AT5G22290.1 51.402 107 44 3 9 115 68 166 1.15e-30 114
Msa0426070 AT5G07680.2 41.333 150 64 4 14 144 53 197 1.30e-30 113
Msa0426070 AT5G07680.1 41.333 150 64 4 14 144 67 211 1.83e-30 113
Msa0426070 AT1G34190.1 52.427 103 49 0 11 113 65 167 2.35e-30 115
Msa0426070 AT5G66300.1 47.959 98 50 1 15 112 66 162 3.73e-30 111
Msa0426070 AT2G33480.3 48.544 103 53 0 10 112 1 103 3.83e-30 109
Msa0426070 AT1G33280.1 50.515 97 45 1 18 114 65 158 4.53e-30 111
Msa0426070 AT3G44290.1 52.336 107 42 3 9 115 61 158 4.85e-30 112
Msa0426070 AT2G24430.2 54.000 100 43 2 19 116 71 169 1.52e-29 110
Msa0426070 AT2G24430.1 54.000 100 43 2 19 116 71 169 1.52e-29 110
Msa0426070 AT2G27300.1 52.041 98 40 2 17 114 68 158 3.75e-29 109
Msa0426070 AT2G33480.1 51.020 98 48 0 15 112 63 160 5.33e-29 107
Msa0426070 AT3G04060.1 44.882 127 57 3 12 130 68 189 7.44e-29 108
Msa0426070 AT4G27410.2 44.068 118 61 1 17 129 67 184 1.40e-28 107
Msa0426070 AT5G18270.2 51.376 109 50 2 11 116 68 176 2.00e-28 107
Msa0426070 AT1G32510.1 47.414 116 53 2 11 118 55 170 2.03e-28 106
Msa0426070 AT5G46590.2 61.728 81 30 1 9 88 53 133 2.06e-28 106
Msa0426070 AT1G69490.1 38.356 146 81 3 17 158 62 202 2.31e-28 106
Msa0426070 AT4G27410.3 44.068 118 61 1 17 129 84 201 2.57e-28 107
Msa0426070 AT5G18270.1 51.887 106 48 2 14 116 71 176 2.68e-28 107
Msa0426070 AT3G10490.4 62.500 80 29 1 36 114 1 80 2.83e-28 107
Msa0426070 AT3G10490.3 62.500 80 29 1 36 114 1 80 2.83e-28 107
Msa0426070 AT3G15170.1 51.376 109 39 3 14 113 70 173 3.12e-28 106
Msa0426070 AT1G56010.1 42.336 137 62 4 19 141 8 141 3.13e-28 105
Msa0426070 AT3G15500.1 52.041 98 43 2 17 112 67 162 3.75e-28 106
Msa0426070 AT3G29035.1 63.889 72 26 0 17 88 77 148 4.65e-28 106
Msa0426070 AT1G52890.1 52.041 98 43 2 17 112 67 162 5.76e-28 106
Msa0426070 AT2G33480.2 51.020 98 46 1 15 112 63 158 7.20e-28 105
Msa0426070 AT1G56010.2 42.336 137 62 4 19 141 75 208 1.07e-27 105
Msa0426070 AT5G09330.5 44.860 107 59 0 7 113 51 157 1.19e-27 107
Msa0426070 AT5G09330.2 44.860 107 59 0 7 113 51 157 1.19e-27 107
Msa0426070 AT5G09330.4 44.860 107 59 0 7 113 51 157 1.19e-27 107
Msa0426070 AT5G09330.3 44.860 107 59 0 7 113 51 157 1.19e-27 107
Msa0426070 AT5G09330.1 44.860 107 59 0 7 113 51 157 1.19e-27 107
Msa0426070 AT5G64060.1 44.762 105 58 0 9 113 53 157 3.02e-27 105
Msa0426070 AT5G53950.1 50.459 109 40 3 14 113 67 170 3.28e-27 105
Msa0426070 AT3G15510.1 43.966 116 52 2 19 121 72 187 5.19e-27 104
Msa0426070 AT2G46770.1 39.286 140 52 3 8 114 43 182 6.53e-27 104
Msa0426070 AT1G32770.1 34.868 152 87 3 18 159 73 222 8.12e-27 103
Msa0426070 AT2G02450.1 53.061 98 41 3 17 112 104 198 1.71e-26 103
Msa0426070 AT1G76420.1 48.544 103 50 2 12 112 70 171 1.78e-26 102
Msa0426070 AT4G28530.1 38.462 156 83 4 12 158 74 225 2.03e-26 102
Msa0426070 AT5G08790.1 41.406 128 65 3 17 137 60 184 2.11e-26 101
Msa0426070 AT2G02450.2 53.061 98 41 3 17 112 104 198 2.50e-26 103
Msa0426070 AT3G61910.1 41.818 110 51 1 18 114 68 177 2.94e-26 102
Msa0426070 AT1G77450.1 46.535 101 46 3 17 112 63 160 5.19e-26 99.8
Msa0426070 AT1G01720.1 47.059 102 45 3 17 112 60 158 8.14e-26 100
Msa0426070 AT3G04070.2 36.875 160 72 6 2 132 37 196 1.32e-25 100
Msa0426070 AT1G79580.3 47.000 100 47 3 18 115 74 169 2.28e-25 100
Msa0426070 AT1G79580.5 47.000 100 47 3 18 115 74 169 2.28e-25 100
Msa0426070 AT1G79580.4 47.000 100 47 3 18 115 74 169 2.28e-25 100
Msa0426070 AT1G79580.2 47.000 100 47 3 18 115 74 169 2.28e-25 100
Msa0426070 AT1G79580.1 47.000 100 47 3 18 115 74 169 2.28e-25 100
Msa0426070 AT5G13180.1 46.939 98 49 2 17 112 64 160 2.63e-25 97.8
Msa0426070 AT1G32870.1 49.524 105 49 2 11 113 59 161 8.46e-25 99.8
Msa0426070 AT1G32870.2 49.524 105 49 2 11 113 59 161 8.73e-25 99.8
Msa0426070 AT1G32870.3 49.524 105 49 2 11 113 95 197 1.07e-24 99.8
Msa0426070 AT5G63790.1 46.602 103 45 3 17 112 103 202 1.39e-24 97.1
Msa0426070 AT5G63790.2 46.602 103 45 3 17 112 113 212 1.50e-24 97.4
Msa0426070 AT1G61110.1 44.545 110 48 3 17 113 69 178 6.49e-24 95.5
Msa0426070 AT3G04070.1 34.659 176 70 7 2 132 37 212 2.82e-23 94.4
Msa0426070 AT2G17040.1 46.465 99 45 2 17 112 59 152 3.05e-23 93.2
Msa0426070 AT5G39820.1 42.017 119 46 4 19 114 75 193 3.62e-23 94.0
Msa0426070 AT2G18060.2 55.714 70 29 1 43 112 3 70 1.52e-22 91.3
Msa0426070 AT1G26870.1 41.284 109 53 2 19 116 71 179 1.72e-22 92.8
Msa0426070 AT1G52880.1 46.364 110 47 3 19 116 72 181 2.51e-21 88.6
Msa0426070 AT2G43000.1 42.424 99 52 3 17 112 71 167 2.68e-21 87.8
Msa0426070 AT4G01520.1 39.474 114 60 3 10 120 54 161 3.98e-21 87.8
Msa0426070 AT4G01550.1 43.363 113 56 2 6 115 49 156 7.54e-21 88.6
Msa0426070 AT4G01540.3 39.474 114 60 3 10 120 54 161 1.22e-20 87.8
Msa0426070 AT4G01540.1 39.474 114 60 3 10 120 54 161 1.67e-20 87.4
Msa0426070 AT4G01540.4 39.474 114 60 3 10 120 54 161 1.71e-20 87.0
Msa0426070 AT4G01540.2 39.474 114 60 3 10 120 54 161 1.96e-20 87.0
Msa0426070 AT5G04395.1 48.750 80 38 1 12 88 89 168 5.48e-20 82.4
Msa0426070 AT5G22290.2 52.703 74 28 2 42 115 1 67 1.99e-19 82.4
Msa0426070 AT2G43000.2 43.182 88 45 3 28 112 4 89 2.92e-18 78.2
Msa0426070 AT5G22380.1 34.783 115 72 2 14 125 61 175 5.89e-18 78.2
Msa0426070 AT3G44350.2 37.143 105 64 2 14 116 60 164 6.89e-18 78.2
Msa0426070 AT3G12910.1 36.364 121 58 3 9 112 67 185 1.33e-17 78.2
Msa0426070 AT3G04420.2 39.623 106 55 2 11 114 54 152 1.88e-17 77.8
Msa0426070 AT3G04420.1 39.623 106 55 2 11 114 54 152 1.90e-17 78.2
Msa0426070 AT3G44350.1 43.421 76 42 1 14 88 60 135 2.72e-16 72.4
Msa0426070 AT1G02230.1 34.711 121 66 5 11 127 54 165 5.57e-14 68.6
Msa0426070 AT1G02220.1 36.000 100 56 2 11 110 54 145 3.22e-13 66.6
Msa0426070 AT1G01010.1 35.088 114 59 3 11 116 54 160 4.72e-12 63.5

Find 55 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAACGGGAGGAGGTCTAAT+TGG 0.260288 3_2:-76489567 Msa0426070:CDS
TGATCGGATTGTATTTCTTC+TGG 0.268816 3_2:+76489302 None:intergenic
CACCACAGTTTGAAACTTCA+AGG 0.314433 3_2:+76489348 None:intergenic
TGGTTCCATCAAGCTCCTTA+AGG 0.335266 3_2:+76489521 None:intergenic
CTGTTCAATTGATTCCCATT+TGG 0.340310 3_2:+76489700 None:intergenic
CTCTGAGATACCAGGGATAA+TGG 0.352390 3_2:+76489226 None:intergenic
AAATCTGGAACCATCTTGAT+TGG 0.370088 3_2:-76489628 Msa0426070:CDS
GAGAAACAAATCTTGGATAC+TGG 0.371022 3_2:-76489678 Msa0426070:CDS
TTTATCTCTTGGTTTGTGTT+GGG 0.376609 3_2:-76489798 None:intergenic
CTTGGATACTGGAAGGCAAC+AGG 0.379088 3_2:-76489667 Msa0426070:CDS
AAGGATCGTAAAATCAAATC+TGG 0.393585 3_2:-76489643 Msa0426070:CDS
GGTTCCATCAAGCTCCTTAA+GGG 0.401742 3_2:+76489522 None:intergenic
CCCACCCTTAAGGAGCTTGA+TGG 0.417142 3_2:-76489526 Msa0426070:CDS
TTGAACAGAGAAACAAATCT+TGG 0.440140 3_2:-76489685 Msa0426070:CDS
CATGAGGTCCAAAATCAAAC+TGG 0.444256 3_2:-76489181 Msa0426070:three_prime_UTR
TTATAGAAATGGACCTGTCC+TGG 0.450131 3_2:+76489496 None:intergenic
CAGGATCGGCAATATCCAAA+TGG 0.459432 3_2:-76489715 Msa0426070:CDS
CGAGTATCGTCCCACCCTTA+AGG 0.461017 3_2:-76489536 Msa0426070:CDS
CAGAGGAAAAGTGATGGATA+TGG 0.461273 3_2:-76489208 Msa0426070:exon
ACATTGGTCGTGCACCTAAC+GGG 0.464123 3_2:-76489582 Msa0426070:CDS
TAACGGGAGGAGGTCTAATT+GGG 0.468912 3_2:-76489566 Msa0426070:CDS
AACTGTAATGGGAGCTTGAT+CGG 0.483644 3_2:+76489286 None:intergenic
TACATTGGTCGTGCACCTAA+CGG 0.487095 3_2:-76489583 Msa0426070:CDS
AAGCAGCTGGCCATTATCCC+TGG 0.495297 3_2:-76489236 Msa0426070:CDS
GTGGTTCTTCTTCTATCCAC+AGG 0.505437 3_2:-76489734 Msa0426070:CDS
CAATTAGACCTCCTCCCGTT+AGG 0.505976 3_2:+76489568 None:intergenic
CTGTGATGTTGAAACTGTAA+TGG 0.513575 3_2:+76489274 None:intergenic
AATCTGGAACCATCTTGATT+GGG 0.516096 3_2:-76489627 Msa0426070:CDS
GAAGTTTCAAACTGTGGTGA+AGG 0.519573 3_2:-76489344 Msa0426070:CDS
AAGTTTCAAACTGTGGTGAA+GGG 0.526196 3_2:-76489343 Msa0426070:CDS
CTTCTTCATCCCAATCAAGA+TGG 0.530609 3_2:+76489618 None:intergenic
TGTGATGTTGAAACTGTAAT+GGG 0.530645 3_2:+76489275 None:intergenic
AGGATCGGCAATATCCAAAT+GGG 0.531502 3_2:-76489714 Msa0426070:CDS
CAGAGAAGAGGATAAGCAGC+TGG 0.546645 3_2:-76489249 Msa0426070:CDS
TCCATCAAGCTCCTTAAGGG+TGG 0.570617 3_2:+76489525 None:intergenic
TATAGAAATGGACCTGTCCT+GGG 0.571357 3_2:+76489497 None:intergenic
TGATGGAACCAACCCAGGAC+AGG 0.574453 3_2:-76489509 Msa0426070:intron
CATTACAGTTTCAACATCAC+AGG 0.574812 3_2:-76489273 Msa0426070:CDS
TTTGGATATTGCCGATCCTG+TGG 0.585066 3_2:+76489718 None:intergenic
TCTTCTTCTATCCACAGGAT+CGG 0.587304 3_2:-76489729 Msa0426070:CDS
GAAATGGACCTGTCCTGGGT+TGG 0.596174 3_2:+76489501 None:intergenic
AACAAATCTTGGATACTGGA+AGG 0.600211 3_2:-76489674 Msa0426070:CDS
AGTTTCAAACTGTGGTGAAG+GGG 0.601390 3_2:-76489342 Msa0426070:CDS
CCATCAAGCTCCTTAAGGGT+GGG 0.602064 3_2:+76489526 None:intergenic
GTCGTGCACCTAACGGGAGG+AGG 0.604113 3_2:-76489576 Msa0426070:CDS
GAGCTTGATGGAACCAACCC+AGG 0.606347 3_2:-76489514 Msa0426070:CDS
AACATCACAGGTCAGAGAAG+AGG 0.609131 3_2:-76489261 Msa0426070:CDS
TTGGATACTGGAAGGCAACA+GGG 0.615969 3_2:-76489666 Msa0426070:CDS
GTATCTCAGAGGAAAAGTGA+TGG 0.620521 3_2:-76489214 Msa0426070:exon
TGATGGATATGGCGCACATG+AGG 0.621299 3_2:-76489197 Msa0426070:three_prime_UTR
AGCCTTGAAGTTTCAAACTG+TGG 0.630939 3_2:-76489350 Msa0426070:CDS
ATACTGGAAGGCAACAGGGA+AGG 0.655689 3_2:-76489662 Msa0426070:CDS
TTGGTCGTGCACCTAACGGG+AGG 0.685469 3_2:-76489579 Msa0426070:CDS
CATTATCCCTGGTATCTCAG+AGG 0.695429 3_2:-76489225 Msa0426070:CDS
TAAAAGACGACGACTCAGAG+TGG 0.709699 3_2:-76489753 Msa0426070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTTGACATAATAAAAAT+AGG + chr3_2:76489470-76489489 None:intergenic 15.0%
!!! AAGACATTAGTTTTCTACAT+TGG - chr3_2:76489334-76489353 Msa0426070:CDS 25.0%
! AGGAGAGAATATTATAGAAA+TGG + chr3_2:76489450-76489469 None:intergenic 25.0%
!!! GCATTGAGTTTTAATCTATT+AGG - chr3_2:76489784-76489803 Msa0426070:CDS 25.0%
TTGAACAGAGAAACAAATCT+TGG - chr3_2:76489247-76489266 Msa0426070:CDS 30.0%
AAGGATCGTAAAATCAAATC+TGG - chr3_2:76489289-76489308 Msa0426070:CDS 30.0%
TGTGATGTTGAAACTGTAAT+GGG + chr3_2:76489660-76489679 None:intergenic 30.0%
!! CTAGTTTTCCAGTTTGATTT+TGG + chr3_2:76489762-76489781 None:intergenic 30.0%
! CTGTTCAATTGATTCCCATT+TGG + chr3_2:76489235-76489254 None:intergenic 35.0%
GAGAAACAAATCTTGGATAC+TGG - chr3_2:76489254-76489273 Msa0426070:CDS 35.0%
AACAAATCTTGGATACTGGA+AGG - chr3_2:76489258-76489277 Msa0426070:CDS 35.0%
! AAATCTGGAACCATCTTGAT+TGG - chr3_2:76489304-76489323 Msa0426070:CDS 35.0%
! AATCTGGAACCATCTTGATT+GGG - chr3_2:76489305-76489324 Msa0426070:CDS 35.0%
GCACATATGCTTTCTGATAT+AGG + chr3_2:76489533-76489552 None:intergenic 35.0%
AAGTTTCAAACTGTGGTGAA+GGG - chr3_2:76489589-76489608 Msa0426070:CDS 35.0%
TGATCGGATTGTATTTCTTC+TGG + chr3_2:76489633-76489652 None:intergenic 35.0%
CTGTGATGTTGAAACTGTAA+TGG + chr3_2:76489661-76489680 None:intergenic 35.0%
CATTACAGTTTCAACATCAC+AGG - chr3_2:76489659-76489678 Msa0426070:CDS 35.0%
TCTTCTTCTATCCACAGGAT+CGG - chr3_2:76489203-76489222 Msa0426070:exon 40.0%
AGGATCGGCAATATCCAAAT+GGG - chr3_2:76489218-76489237 Msa0426070:CDS 40.0%
CTTCTTCATCCCAATCAAGA+TGG + chr3_2:76489317-76489336 None:intergenic 40.0%
TATAGAAATGGACCTGTCCT+GGG + chr3_2:76489438-76489457 None:intergenic 40.0%
TTATAGAAATGGACCTGTCC+TGG + chr3_2:76489439-76489458 None:intergenic 40.0%
AGCCTTGAAGTTTCAAACTG+TGG - chr3_2:76489582-76489601 Msa0426070:CDS 40.0%
! CACCACAGTTTGAAACTTCA+AGG + chr3_2:76489587-76489606 None:intergenic 40.0%
GAAGTTTCAAACTGTGGTGA+AGG - chr3_2:76489588-76489607 Msa0426070:CDS 40.0%
AGTTTCAAACTGTGGTGAAG+GGG - chr3_2:76489590-76489609 Msa0426070:CDS 40.0%
! AACTGTAATGGGAGCTTGAT+CGG + chr3_2:76489649-76489668 None:intergenic 40.0%
!!! ACTTTTCCTCTGAGATACCA+GGG + chr3_2:76489716-76489735 None:intergenic 40.0%
GTATCTCAGAGGAAAAGTGA+TGG - chr3_2:76489718-76489737 Msa0426070:CDS 40.0%
!! CAGAGGAAAAGTGATGGATA+TGG - chr3_2:76489724-76489743 Msa0426070:CDS 40.0%
CATGAGGTCCAAAATCAAAC+TGG - chr3_2:76489751-76489770 Msa0426070:CDS 40.0%
TAAAAGACGACGACTCAGAG+TGG - chr3_2:76489179-76489198 Msa0426070:three_prime_UTR 45.0%
GTGGTTCTTCTTCTATCCAC+AGG - chr3_2:76489198-76489217 Msa0426070:exon 45.0%
TTTGGATATTGCCGATCCTG+TGG + chr3_2:76489217-76489236 None:intergenic 45.0%
CAGGATCGGCAATATCCAAA+TGG - chr3_2:76489217-76489236 Msa0426070:CDS 45.0%
TTGGATACTGGAAGGCAACA+GGG - chr3_2:76489266-76489285 Msa0426070:CDS 45.0%
TACATTGGTCGTGCACCTAA+CGG - chr3_2:76489349-76489368 Msa0426070:CDS 45.0%
TAACGGGAGGAGGTCTAATT+GGG - chr3_2:76489366-76489385 Msa0426070:CDS 45.0%
GGTTCCATCAAGCTCCTTAA+GGG + chr3_2:76489413-76489432 None:intergenic 45.0%
TGGTTCCATCAAGCTCCTTA+AGG + chr3_2:76489414-76489433 None:intergenic 45.0%
AACATCACAGGTCAGAGAAG+AGG - chr3_2:76489671-76489690 Msa0426070:CDS 45.0%
!! CTCTGAGATACCAGGGATAA+TGG + chr3_2:76489709-76489728 None:intergenic 45.0%
CATTATCCCTGGTATCTCAG+AGG - chr3_2:76489707-76489726 Msa0426070:CDS 45.0%
!!! CACTTTTCCTCTGAGATACC+AGG + chr3_2:76489717-76489736 None:intergenic 45.0%
CTTGGATACTGGAAGGCAAC+AGG - chr3_2:76489265-76489284 Msa0426070:CDS 50.0%
ATACTGGAAGGCAACAGGGA+AGG - chr3_2:76489270-76489289 Msa0426070:CDS 50.0%
ACATTGGTCGTGCACCTAAC+GGG - chr3_2:76489350-76489369 Msa0426070:CDS 50.0%
CAATTAGACCTCCTCCCGTT+AGG + chr3_2:76489367-76489386 None:intergenic 50.0%
CTAACGGGAGGAGGTCTAAT+TGG - chr3_2:76489365-76489384 Msa0426070:CDS 50.0%
CCATCAAGCTCCTTAAGGGT+GGG + chr3_2:76489409-76489428 None:intergenic 50.0%
TCCATCAAGCTCCTTAAGGG+TGG + chr3_2:76489410-76489429 None:intergenic 50.0%
CAGAGAAGAGGATAAGCAGC+TGG - chr3_2:76489683-76489702 Msa0426070:CDS 50.0%
! TGATGGATATGGCGCACATG+AGG - chr3_2:76489735-76489754 Msa0426070:CDS 50.0%
! CGAGTATCGTCCCACCCTTA+AGG - chr3_2:76489396-76489415 Msa0426070:intron 55.0%
CCCACCCTTAAGGAGCTTGA+TGG - chr3_2:76489406-76489425 Msa0426070:intron 55.0%
GAGCTTGATGGAACCAACCC+AGG - chr3_2:76489418-76489437 Msa0426070:intron 55.0%
TGATGGAACCAACCCAGGAC+AGG - chr3_2:76489423-76489442 Msa0426070:intron 55.0%
GAAATGGACCTGTCCTGGGT+TGG + chr3_2:76489434-76489453 None:intergenic 55.0%
AAGCAGCTGGCCATTATCCC+TGG - chr3_2:76489696-76489715 Msa0426070:CDS 55.0%
TTGGTCGTGCACCTAACGGG+AGG - chr3_2:76489353-76489372 Msa0426070:CDS 60.0%
GTCGTGCACCTAACGGGAGG+AGG - chr3_2:76489356-76489375 Msa0426070:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 76489142 76489812 76489142 ID=Msa0426070;Name=Msa0426070
chr3_2 mRNA 76489142 76489812 76489142 ID=Msa0426070-mRNA-1;Parent=Msa0426070;Name=Msa0426070-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|1|0.5|1|1|1|2|75|166
chr3_2 exon 76489142 76489414 76489142 ID=Msa0426070-mRNA-1:exon:19804;Parent=Msa0426070-mRNA-1
chr3_2 exon 76489510 76489812 76489510 ID=Msa0426070-mRNA-1:exon:19803;Parent=Msa0426070-mRNA-1
chr3_2 CDS 76489510 76489812 76489510 ID=Msa0426070-mRNA-1:cds;Parent=Msa0426070-mRNA-1
chr3_2 CDS 76489217 76489414 76489217 ID=Msa0426070-mRNA-1:cds;Parent=Msa0426070-mRNA-1
chr3_2 three_prime_UTR 76489142 76489216 76489142 ID=Msa0426070-mRNA-1:three_prime_utr;Parent=Msa0426070-mRNA-1
Gene Sequence

>Msa0426070

TTGGTTTGTGTTGGGTTTGTTGTAGATTTGTCAGTGATAAAAGACGACGACTCAGAGTGGTTCTTCTTCTATCCACAGGATCGGCAATATCCAAATGGGAATCAATTGAACAGAGAAACAAATCTTGGATACTGGAAGGCAACAGGGAAGGATCGTAAAATCAAATCTGGAACCATCTTGATTGGGATGAAGAAGACATTAGTTTTCTACATTGGTCGTGCACCTAACGGGAGGAGGTCTAATTGGGTAATGCACGAGTATCGTCCCACCCTTAAGGAGCTTGATGGAACCAACCCAGGACAGAAAGCATATGTGCTATGTCGATTATTTAAGAAAAACGACGAGAGCCTTGAAGTTTCAAACTGTGGTGAAGGGGAGCAGACTAATTCAGCTCCAGAAGAAATACAATCCGATCAAGCTCCCATTACAGTTTCAACATCACAGGTCAGAGAAGAGGATAAGCAGCTGGCCATTATCCCTGGTATCTCAGAGGAAAAGTGA
Protein sequence

>Msa0426070

LVCVGFVVDLSVIKDDDSEWFFFYPQDRQYPNGNQLNRETNLGYWKATGKDRKIKSGTILIGMKKTLVFYIGRAPNGRRSNWVMHEYRPTLKELDGTNPGQKAYVLCRLFKKNDESLEVSNCGEGEQTNSAPEEIQSDQAPITVSTSQVREEDKQLAIIPGISEEK*