AlfalfaGEDB | Msa0430550

M. sativa cultivar zhongmu-4 / Msa0430550

KEGG: K14491
GO: GO:0000156 GO:0000160 GO:0001101 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009888 GO:0009889 GO:0009966 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010073 GO:0010074 GO:0010075 GO:0010082 GO:0010380 GO:0010468 GO:0010492 GO:0010556 GO:0010646 GO:0019219 GO:0019222 GO:0019827 GO:0022622 GO:0023051 GO:0023052 GO:0031323 GO:0031326 GO:0031537 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048364 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048583 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051193 GO:0051239 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071495 GO:0080022 GO:0080036 GO:0080050 GO:0080090 GO:0080113 GO:0090056 GO:0098727 GO:0099402 GO:0140110 GO:1901401 GO:1901463 GO:1901700 GO:1903506 GO:1905392 GO:2000026 GO:2000112 GO:2000241 GO:2000280 GO:2001141
PFAM: PF00072.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0430550	KEH35772.1	95.897	195	7	1	1	194	1	195	1.43e-121	352

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0430550	sp\|Q6H805\|ORR24_ORYSJ	54.583	240	55	2	6	191	23	262	8.87e-77	244

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0430550	A0A072V1R9	95.897	195	7	1	1	194	1	195	6.85e-122	352

TFs/TRs:

Gene ID	Type	Classification
Msa0430550	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0430550	MtrunA17_Chr3g0134461	95.897	195	7	1	1	194	1	195	1.32e-125	352
Msa0430550	MtrunA17_Chr3g0134481	72.165	194	44	1	1	194	1	184	3.55e-93	270
Msa0430550	MtrunA17_Chr1g0149841	52.720	239	61	2	5	191	2	240	5.58e-77	238
Msa0430550	MtrunA17_Chr7g0275401	47.917	240	72	1	5	191	2	241	1.58e-70	216
Msa0430550	MtrunA17_Chr7g0275891	47.222	252	68	4	5	191	2	253	4.64e-69	214
Msa0430550	MtrunA17_Chr4g0067981	49.372	239	71	2	3	191	26	264	8.18e-69	221
Msa0430550	MtrunA17_Chr1g0149851	46.457	254	69	3	5	191	2	255	1.26e-65	210
Msa0430550	MtrunA17_Chr2g0294911	46.809	235	77	2	5	191	26	260	1.37e-65	211
Msa0430550	MtrunA17_Chr3g0137101	75.373	134	33	0	1	134	1	134	7.47e-64	207
Msa0430550	MtrunA17_Chr3g0137101	81.356	59	11	0	133	191	189	247	9.66e-25	100
Msa0430550	MtrunA17_Chr1g0161431	46.091	243	76	2	4	191	7	249	5.37e-63	205
Msa0430550	MtrunA17_Chr4g0074411	46.025	239	80	1	2	191	12	250	3.73e-62	202
Msa0430550	MtrunA17_Chr4g0074401	46.610	236	74	1	8	191	17	252	1.13e-61	201
Msa0430550	MtrunA17_Chr3g0123131	44.492	236	79	2	8	191	13	248	6.13e-61	199
Msa0430550	MtrunA17_Chr8g0376881	45.339	236	79	2	6	191	23	258	8.59e-59	192
Msa0430550	MtrunA17_Chr2g0304841	64.122	131	47	0	2	132	12	142	4.39e-51	174
Msa0430550	MtrunA17_Chr2g0304841	68.519	54	17	0	138	191	209	262	3.70e-17	79.0
Msa0430550	MtrunA17_Chr4g0009851	40.187	214	102	3	3	191	6	218	2.82e-49	162
Msa0430550	MtrunA17_Chr4g0074421	46.961	181	49	1	58	191	2	182	3.42e-48	155
Msa0430550	MtrunA17_Chr1g0149831	46.893	177	39	3	67	191	1	174	6.63e-44	145
Msa0430550	MtrunA17_Chr4g0009901	37.321	209	105	3	8	191	10	217	3.15e-41	140
Msa0430550	MtrunA17_Chr6g0471041	33.491	212	115	3	7	193	13	223	1.06e-32	121
Msa0430550	MtrunA17_Chr4g0009981	36.585	164	79	2	53	191	2	165	6.28e-32	115
Msa0430550	MtrunA17_Chr8g0391911	29.832	238	113	6	8	193	9	244	8.93e-27	102
Msa0430550	MtrunA17_Chr8g0383961	35.811	148	92	2	1	145	7	154	1.57e-22	91.7
Msa0430550	MtrunA17_Chr4g0011071	32.374	139	69	2	55	168	36	174	4.69e-22	88.2
Msa0430550	MtrunA17_Chr6g0456631	33.594	128	82	2	5	130	6	132	7.68e-22	87.4
Msa0430550	MtrunA17_Chr5g0400241	39.200	125	70	3	8	130	14	134	8.97e-20	86.3
Msa0430550	MtrunA17_Chr5g0400241	52.632	57	27	0	135	191	304	360	2.19e-12	64.7
Msa0430550	MtrunA17_Chr4g0009991	37.815	119	50	2	100	194	1	119	1.26e-19	80.5
Msa0430550	MtrunA17_Chr3g0127941	34.677	124	76	2	12	131	56	178	5.15e-19	84.3
Msa0430550	MtrunA17_Chr7g0276361	32.450	151	96	3	1	145	1	151	5.57e-19	84.0
Msa0430550	MtrunA17_Chr8g0345901	36.667	120	73	1	14	130	38	157	5.68e-19	84.0
Msa0430550	MtrunA17_Chr3g0091641	32.258	124	81	1	12	132	24	147	5.99e-18	80.9
Msa0430550	MtrunA17_Chr1g0181811	31.690	142	94	1	12	150	85	226	1.38e-17	80.1
Msa0430550	MtrunA17_Chr4g0061021	31.452	124	82	1	12	132	33	156	1.53e-17	79.7
Msa0430550	MtrunA17_Chr6g0473731	30.370	135	63	1	88	191	10	144	1.70e-17	79.7
Msa0430550	MtrunA17_Chr4g0054981	33.333	123	80	1	8	130	14	134	2.40e-17	79.3
Msa0430550	MtrunA17_Chr4g0054981	51.515	66	29	2	127	191	296	359	3.35e-12	64.3
Msa0430550	MtrunA17_Chr4g0030271	35.246	122	76	2	12	130	89	210	9.36e-17	77.8
Msa0430550	MtrunA17_Chr5g0423031	37.037	81	50	1	8	88	10	89	7.91e-15	69.3
Msa0430550	MtrunA17_Chr8g0350761	32.773	119	79	1	11	128	20	138	1.37e-13	68.2
Msa0430550	MtrunA17_Chr4g0010001	35.802	81	51	1	8	88	10	89	1.75e-12	60.8
Msa0430550	MtrunA17_Chr4g0040481	47.170	53	28	0	139	191	155	207	2.51e-11	61.6
Msa0430550	MtrunA17_Chr4g0031961	52.941	51	24	0	141	191	130	180	5.38e-11	60.5
Msa0430550	MtrunA17_Chr2g0324891	47.170	53	28	0	139	191	158	210	5.73e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0430550	AT4G31920.1	54.054	222	66	1	6	191	11	232	5.72e-77	240
Msa0430550	AT2G25180.1	53.165	237	63	2	3	191	8	244	1.23e-76	241
Msa0430550	AT5G58080.1	46.414	237	79	2	2	191	8	243	6.43e-61	200
Msa0430550	AT1G67710.1	46.186	236	74	3	9	191	8	243	5.50e-60	195
Msa0430550	AT2G01760.1	40.650	246	87	3	5	191	4	249	6.43e-53	174
Msa0430550	AT2G01760.2	36.496	274	87	4	5	191	4	277	6.87e-47	159
Msa0430550	AT3G16857.1	53.103	145	56	1	8	152	33	165	8.49e-45	157
Msa0430550	AT3G16857.1	56.410	78	24	1	124	191	209	286	1.13e-19	86.7
Msa0430550	AT3G16857.2	53.103	145	56	1	8	152	33	165	8.68e-45	157
Msa0430550	AT3G16857.2	56.410	78	24	1	124	191	209	286	1.06e-19	86.7
Msa0430550	AT5G49240.1	31.950	241	113	2	5	194	35	275	7.63e-38	132
Msa0430550	AT1G67710.2	43.820	178	47	3	67	191	1	178	2.06e-36	132
Msa0430550	AT3G62670.1	34.177	237	107	5	2	191	31	265	6.34e-34	125
Msa0430550	AT3G16857.3	53.774	106	37	1	47	152	2	95	4.07e-32	122
Msa0430550	AT3G16857.3	56.410	78	24	1	124	191	139	216	1.18e-19	86.3
Msa0430550	AT1G49190.1	30.833	240	111	5	7	191	29	268	6.95e-31	116
Msa0430550	AT1G49190.2	38.400	125	77	0	7	131	29	153	2.44e-25	102
Msa0430550	AT1G67710.3	39.873	158	42	3	87	191	12	169	2.95e-25	101
Msa0430550	AT2G46790.3	34.815	135	85	1	12	143	37	171	3.90e-20	87.4
Msa0430550	AT5G61380.1	33.871	124	79	1	12	132	19	142	4.41e-20	87.8
Msa0430550	AT2G46790.1	34.815	135	85	1	12	143	37	171	4.48e-20	87.4
Msa0430550	AT5G24470.1	34.400	125	79	1	10	131	48	172	1.11e-19	86.3
Msa0430550	AT2G01760.3	51.515	66	31	1	67	131	1	66	4.92e-17	77.8
Msa0430550	AT2G01760.3	66.667	51	17	0	141	191	134	184	3.07e-16	75.5
Msa0430550	AT5G02810.1	36.066	122	75	1	12	130	78	199	1.14e-16	77.8
Msa0430550	AT5G60100.7	35.246	122	76	1	12	130	64	185	2.00e-16	76.6
Msa0430550	AT5G60100.6	35.246	122	76	1	12	130	64	185	2.00e-16	76.6
Msa0430550	AT5G60100.4	35.246	122	76	1	12	130	64	185	2.00e-16	76.6
Msa0430550	AT5G60100.2	35.246	122	76	1	12	130	64	185	2.22e-16	77.0
Msa0430550	AT5G60100.5	35.246	122	76	1	12	130	64	185	4.36e-16	75.9
Msa0430550	AT5G60100.3	35.246	122	76	1	12	130	64	185	4.36e-16	75.9
Msa0430550	AT5G60100.1	35.246	122	76	1	12	130	64	185	4.36e-16	75.9
Msa0430550	AT4G18020.3	34.091	132	78	3	6	130	12	141	2.88e-15	73.6
Msa0430550	AT4G18020.3	46.970	66	33	1	128	191	280	345	4.72e-11	61.2
Msa0430550	AT4G18020.2	34.091	132	78	3	6	130	12	141	2.88e-15	73.6
Msa0430550	AT4G18020.2	46.970	66	33	1	128	191	280	345	4.72e-11	61.2
Msa0430550	AT4G18020.1	34.091	132	78	3	6	130	12	141	2.88e-15	73.6
Msa0430550	AT4G18020.1	46.970	66	33	1	128	191	280	345	4.72e-11	61.2
Msa0430550	AT4G18020.6	34.091	132	78	3	6	130	12	141	2.88e-15	73.6
Msa0430550	AT4G18020.6	46.970	66	33	1	128	191	280	345	4.72e-11	61.2
Msa0430550	AT4G18020.9	34.091	132	78	3	6	130	12	141	2.88e-15	73.6
Msa0430550	AT4G18020.9	46.970	66	33	1	128	191	280	345	4.72e-11	61.2
Msa0430550	AT4G18020.8	34.091	132	78	3	6	130	12	141	2.97e-15	73.6
Msa0430550	AT4G18020.8	46.970	66	33	1	128	191	280	345	4.85e-11	61.2
Msa0430550	AT4G18020.11	34.091	132	78	3	6	130	12	141	2.97e-15	73.6
Msa0430550	AT4G18020.11	46.970	66	33	1	128	191	280	345	4.85e-11	61.2
Msa0430550	AT4G18020.4	34.091	132	78	3	6	130	12	141	2.97e-15	73.6
Msa0430550	AT4G18020.4	46.970	66	33	1	128	191	280	345	4.85e-11	61.2
Msa0430550	AT4G18020.5	34.091	132	78	3	6	130	12	141	2.97e-15	73.6
Msa0430550	AT4G18020.5	46.970	66	33	1	128	191	280	345	4.85e-11	61.2
Msa0430550	AT4G18020.10	34.091	132	78	3	6	130	12	141	2.97e-15	73.6
Msa0430550	AT4G18020.10	46.970	66	33	1	128	191	280	345	4.85e-11	61.2
Msa0430550	AT4G18020.7	34.091	132	78	3	6	130	12	141	2.97e-15	73.6
Msa0430550	AT4G18020.7	46.970	66	33	1	128	191	280	345	4.85e-11	61.2
Msa0430550	AT5G07210.2	29.861	144	89	3	12	154	16	148	2.23e-14	71.2
Msa0430550	AT5G44190.2	57.692	52	22	0	140	191	145	196	4.36e-13	67.0
Msa0430550	AT5G44190.1	57.692	52	22	0	140	191	145	196	5.16e-13	67.0
Msa0430550	AT3G46640.2	52.542	59	26	1	135	191	135	193	8.15e-12	63.2
Msa0430550	AT3G46640.1	52.542	59	26	1	135	191	135	193	8.15e-12	63.2
Msa0430550	AT5G07210.1	28.289	152	89	4	12	154	16	156	8.41e-12	63.5
Msa0430550	AT3G46640.3	52.542	59	26	1	135	191	135	193	8.76e-12	63.2
Msa0430550	AT5G59570.2	50.847	59	27	1	135	191	132	190	2.19e-11	62.0
Msa0430550	AT5G59570.1	50.847	59	27	1	135	191	132	190	2.19e-11	62.0
Msa0430550	AT2G40970.1	52.941	51	24	0	141	191	104	154	2.69e-11	61.2
Msa0430550	AT2G20570.1	52.830	53	25	0	139	191	150	202	3.13e-11	61.6
Msa0430550	AT2G20570.2	60.000	45	18	0	147	191	174	218	5.27e-11	61.2

Find 80 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCATGGTGCCTCTGAGTTTC+TGG	0.051652	3_2:+82440082	Msa0430550:CDS
TTTCTTTCACTGAATGTTTC+TGG	0.116372	3_2:+82440652	Msa0430550:three_prime_UTR
ACCAATCATAATTTGCTCTT+TGG	0.298362	3_2:-82440984	None:intergenic
TTGCTCTTTGGCGGAATAGA+AGG	0.330157	3_2:-82440972	None:intergenic
TAGTAGAGAAGAGAAAATTA+TGG	0.377587	3_2:+82439084	Msa0430550:exon
GTTGTACCCGAAAAGATTCT+TGG	0.381343	3_2:+82440532	Msa0430550:CDS
TAGAGGAGCTAAAGACCATT+TGG	0.400234	3_2:+82440120	Msa0430550:CDS
AGCCTCAAGGAGTGCAGATC+AGG	0.403817	3_2:+82440771	Msa0430550:three_prime_UTR
AATGTTTCTGGTTTATGAAA+TGG	0.414593	3_2:+82440664	Msa0430550:three_prime_UTR
GCCAAAGAGCAAATTATGAT+TGG	0.416480	3_2:+82440983	Msa0430550:three_prime_UTR
AGGACGTGGCAAGCCATCTC+CGG	0.418300	3_2:+82440584	Msa0430550:CDS
CCATTTGGCAGCATGTAATT+CGG	0.418909	3_2:+82440135	Msa0430550:CDS
AAACCAACACGCTAGTAGTT+TGG	0.419478	3_2:+82440744	Msa0430550:three_prime_UTR
AGAAGAAGTCCCAGTCTGTT+TGG	0.426238	3_2:+82440189	Msa0430550:CDS
CACGCATTCCGATGGGAAAC+CGG	0.429749	3_2:-82439120	None:intergenic
ACATTTACAGACCAAACAAC+CGG	0.435815	3_2:-82440862	None:intergenic
GGAACTTGAGACGGACCTTC+CGG	0.436170	3_2:+82439887	Msa0430550:CDS
GGTGAAACCTGTGAGATTAG+AGG	0.438440	3_2:+82440103	Msa0430550:CDS
AATCATAATTTGCTCTTTGG+CGG	0.440127	3_2:-82440981	None:intergenic
GTTGCTGCTGTTAATCAGTT+AGG	0.449852	3_2:+82440236	Msa0430550:CDS
CCGAATTACATGCTGCCAAA+TGG	0.458584	3_2:-82440135	None:intergenic
AGCTTAAGTCCATCCATGTC+AGG	0.462326	3_2:-82439852	None:intergenic
TCCGATGGGAAACCGGTCAC+TGG	0.476693	3_2:-82439113	None:intergenic
TTTAGCTCCTCTAATCTCAC+AGG	0.481770	3_2:-82440110	None:intergenic
AAGAGCAAATTATGATTGGT+AGG	0.503759	3_2:+82440987	Msa0430550:three_prime_UTR
GGACGTGGCAAGCCATCTCC+GGG	0.509409	3_2:+82440585	Msa0430550:CDS
GGGGCTTCTTCTGAGCTCAT+GGG	0.512861	3_2:-82440840	None:intergenic
ACTAGAAAATTGCCCGGAGA+TGG	0.513115	3_2:-82440597	None:intergenic
GTCCCAGTCTGTTTGGTCTG+TGG	0.513687	3_2:+82440196	Msa0430550:CDS
CAACGCCGGATCTCTAAACT+AGG	0.514698	3_2:-82441079	None:intergenic
ATGCGTGTTCTCGCCGTCGA+CGG	0.517593	3_2:+82439136	Msa0430550:CDS
CGATAATGAGCTAGTGATGA+AGG	0.518138	3_2:+82440052	Msa0430550:CDS
AAAGATGAGGATAAAGAGAA+TGG	0.519948	3_2:+82440802	Msa0430550:three_prime_UTR
GCTCCACAGACCAAACAGAC+TGG	0.522152	3_2:-82440199	None:intergenic
TTCTAAATCCGAGAGATAAG+TGG	0.525410	3_2:-82439164	None:intergenic
GGGACTTCTTCTGAGCTGAT+AGG	0.528115	3_2:-82440178	None:intergenic
TTAGATAAATTAAAGCGTAA+AGG	0.528607	3_2:-82440620	None:intergenic
GGTGCACATCAGCGATTACC+AGG	0.528972	3_2:-82439827	None:intergenic
CGGCGATTCCACTTATCTCT+CGG	0.532887	3_2:+82439156	Msa0430550:CDS
ACTTAAGCTGCTTGAACTTG+TGG	0.534364	3_2:+82439866	Msa0430550:CDS
GGCGAGAACACGCATTCCGA+TGG	0.535415	3_2:-82439128	None:intergenic
CTCCACAGACCAAACAGACT+GGG	0.537731	3_2:-82440198	None:intergenic
GTGCACCTGCCTGACATGGA+TGG	0.546404	3_2:+82439843	Msa0430550:CDS
AAATATTACAAGGGAGGACG+TGG	0.547028	3_2:+82440570	Msa0430550:CDS
GAACTGAACTTACTTACGAC+CGG	0.554201	3_2:-82439906	None:intergenic
AAAGTTAGCTCTGTGGGCTG+TGG	0.555004	3_2:+82440720	Msa0430550:three_prime_UTR
GTGAAGAAAGTTAGCTCTGT+GGG	0.556660	3_2:+82440714	Msa0430550:three_prime_UTR
CATTTACAGACCAAACAACC+GGG	0.558862	3_2:-82440861	None:intergenic
TTATGGATGACTCCAGTGAC+CGG	0.561206	3_2:+82439101	Msa0430550:exon
GCATGTAATTCGGAAGAAGA+AGG	0.566666	3_2:+82440145	Msa0430550:CDS
TGAACTTGTGGAACTTGAGA+CGG	0.569359	3_2:+82439878	Msa0430550:CDS
TGATGTGCACCTGCCTGACA+TGG	0.573968	3_2:+82439839	Msa0430550:CDS
TAAGTCCATCCATGTCAGGC+AGG	0.574028	3_2:-82439848	None:intergenic
CTCTTTGGCGGAATAGAAGG+AGG	0.575118	3_2:-82440969	None:intergenic
TCCAGTGACCGGTTTCCCAT+CGG	0.576158	3_2:+82439112	Msa0430550:CDS
GTAGTTTGGTGGCAGCCTCA+AGG	0.578073	3_2:+82440758	Msa0430550:three_prime_UTR
TGAATGTTGAAAATATTACA+AGG	0.578744	3_2:+82440560	Msa0430550:CDS
CCAACACGCTAGTAGTTTGG+TGG	0.581455	3_2:+82440747	Msa0430550:three_prime_UTR
ATCCTGATCTGCACTCCTTG+AGG	0.584552	3_2:-82440773	None:intergenic
GACCGTGGTATTGACATGAT+CGG	0.585397	3_2:-82439195	None:intergenic
ATGAGCTCAGAAGAAGCCCC+CGG	0.587881	3_2:+82440843	Msa0430550:three_prime_UTR
CCACCAAACTACTAGCGTGT+TGG	0.591206	3_2:-82440747	None:intergenic
ATAAGTGGAATCGCCGTCGA+CGG	0.593520	3_2:-82439149	None:intergenic
TAAAGGACTAGAAAATTGCC+CGG	0.605859	3_2:-82440603	None:intergenic
GCGAGAACACGCATTCCGAT+GGG	0.617268	3_2:-82439127	None:intergenic
TGAACTTACTTACGACCGGA+AGG	0.625105	3_2:-82439902	None:intergenic
TCAGGATGAAGACAAAGATG+AGG	0.628542	3_2:+82440789	Msa0430550:three_prime_UTR
CCAGAAACTCAGAGGCACCA+TGG	0.637813	3_2:-82440082	None:intergenic
GAATGTTGAAAATATTACAA+GGG	0.643931	3_2:+82440561	Msa0430550:CDS
TAACAGCAGCAACAAACTTG+TGG	0.645143	3_2:-82440226	None:intergenic
TCGGAAGAAGAAGGACAATG+AGG	0.649373	3_2:+82440154	Msa0430550:CDS
TGTTGAAAATATTACAAGGG+AGG	0.651126	3_2:+82440564	Msa0430550:CDS
AAACAAAGACAAGTTTGACC+TGG	0.671629	3_2:+82439809	Msa0430550:CDS
GTGATGAAGGCAGTTTACCA+TGG	0.674402	3_2:+82440065	Msa0430550:CDS
CTCCGATCATGTCAATACCA+CGG	0.699650	3_2:+82439193	Msa0430550:CDS
TGTGAAGAAAGTTAGCTCTG+TGG	0.699756	3_2:+82440713	Msa0430550:three_prime_UTR
TTTACAGACCAAACAACCGG+GGG	0.705017	3_2:-82440859	None:intergenic
CGTGGTATTGACATGATCGG+AGG	0.711493	3_2:-82439192	None:intergenic
AGGTTTCACCAGAAACTCAG+AGG	0.716031	3_2:-82440090	None:intergenic
ATTTACAGACCAAACAACCG+GGG	0.719728	3_2:-82440860	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGAATAAATCAAATAAAATT+GGG	-	chr3_2:82439241-82439260	None:intergenic	10.0%
!!	ATGAATAAATCAAATAAAAT+TGG	-	chr3_2:82439242-82439261	None:intergenic	10.0%
!!!	ATATTTATGTTATTTATGTA+TGG	+	chr3_2:82439727-82439746	Msa0430550:intron	10.0%
!!	AAAAAAAATTATAACAACAA+AGG	-	chr3_2:82440006-82440025	None:intergenic	10.0%
!!!	TTTTTTTTTATTTTTATTGG+AGG	+	chr3_2:82440913-82440932	Msa0430550:three_prime_UTR	10.0%
!!	TGAATAAATCAAATAAAATT+GGG	-	chr3_2:82439241-82439260	None:intergenic	10.0%
!!	ATGAATAAATCAAATAAAAT+TGG	-	chr3_2:82439242-82439261	None:intergenic	10.0%
!!!	ATATTTATGTTATTTATGTA+TGG	+	chr3_2:82439727-82439746	Msa0430550:intron	10.0%
!!	AAAAAAAATTATAACAACAA+AGG	-	chr3_2:82440006-82440025	None:intergenic	10.0%
!!!	TTTTTTTTTATTTTTATTGG+AGG	+	chr3_2:82440913-82440932	Msa0430550:three_prime_UTR	10.0%
!!!	TATGTTATTTATACACATTT+TGG	+	chr3_2:82439607-82439626	Msa0430550:intron	15.0%
!!	GTAAAATGAGAAATAAAATT+CGG	-	chr3_2:82440335-82440354	None:intergenic	15.0%
!!!	TATGTTATTTATACACATTT+TGG	+	chr3_2:82439607-82439626	Msa0430550:intron	15.0%
!!	GTAAAATGAGAAATAAAATT+CGG	-	chr3_2:82440335-82440354	None:intergenic	15.0%
!!	TGATCATAATTGATAAGTAA+AGG	+	chr3_2:82439295-82439314	Msa0430550:intron	20.0%
!!!	TAGATATGATTTTAGTTTGT+TGG	+	chr3_2:82439360-82439379	Msa0430550:intron	20.0%
!!!	TTATGTTATTTATGTATGGT+TGG	+	chr3_2:82439731-82439750	Msa0430550:intron	20.0%
!!!	TATGTTATTTATGTATGGTT+GGG	+	chr3_2:82439732-82439751	Msa0430550:intron	20.0%
!!	ATTATTGATCAATGATTGTT+TGG	+	chr3_2:82439976-82439995	Msa0430550:intron	20.0%
!!	AATAAAAATTGTTTGTGTTG+CGG	-	chr3_2:82440288-82440307	None:intergenic	20.0%
!!!	TTTAATTTATTTTCAATCCG+AGG	+	chr3_2:82440370-82440389	Msa0430550:intron	20.0%
!!	TGAAAAAAACTTATGATGAT+AGG	+	chr3_2:82440394-82440413	Msa0430550:intron	20.0%
!!	TGAATGTTGAAAATATTACA+AGG	+	chr3_2:82440560-82440579	Msa0430550:CDS	20.0%
!!	GAATGTTGAAAATATTACAA+GGG	+	chr3_2:82440561-82440580	Msa0430550:CDS	20.0%
!!	TTAGATAAATTAAAGCGTAA+AGG	-	chr3_2:82440623-82440642	None:intergenic	20.0%
!!!	CTAGGTTTTTAAATATAACA+AGG	-	chr3_2:82441064-82441083	None:intergenic	20.0%
!!	TGATCATAATTGATAAGTAA+AGG	+	chr3_2:82439295-82439314	Msa0430550:intron	20.0%
!!!	TAGATATGATTTTAGTTTGT+TGG	+	chr3_2:82439360-82439379	Msa0430550:intron	20.0%
!!!	TTATGTTATTTATGTATGGT+TGG	+	chr3_2:82439731-82439750	Msa0430550:intron	20.0%
!!!	TATGTTATTTATGTATGGTT+GGG	+	chr3_2:82439732-82439751	Msa0430550:intron	20.0%
!!	ATTATTGATCAATGATTGTT+TGG	+	chr3_2:82439976-82439995	Msa0430550:intron	20.0%
!!	AATAAAAATTGTTTGTGTTG+CGG	-	chr3_2:82440288-82440307	None:intergenic	20.0%
!!!	TTTAATTTATTTTCAATCCG+AGG	+	chr3_2:82440370-82440389	Msa0430550:intron	20.0%
!!	TGAAAAAAACTTATGATGAT+AGG	+	chr3_2:82440394-82440413	Msa0430550:intron	20.0%
!!	TGAATGTTGAAAATATTACA+AGG	+	chr3_2:82440560-82440579	Msa0430550:CDS	20.0%
!!	GAATGTTGAAAATATTACAA+GGG	+	chr3_2:82440561-82440580	Msa0430550:CDS	20.0%
!!	TTAGATAAATTAAAGCGTAA+AGG	-	chr3_2:82440623-82440642	None:intergenic	20.0%
!!!	CTAGGTTTTTAAATATAACA+AGG	-	chr3_2:82441064-82441083	None:intergenic	20.0%
!	TAGTAGAGAAGAGAAAATTA+TGG	+	chr3_2:82439084-82439103	Msa0430550:exon	25.0%
!	CATGCATTATACACATAAAT+TGG	-	chr3_2:82439274-82439293	None:intergenic	25.0%
!	TATGTATATGTAGAGAGATA+CGG	+	chr3_2:82439319-82439338	Msa0430550:intron	25.0%
!	CACATTTATAAACACTATAG+AGG	-	chr3_2:82439460-82439479	None:intergenic	25.0%
!	TGAATTCAAAGTAAGTTCTT+TGG	-	chr3_2:82439499-82439518	None:intergenic	25.0%
!	GAACTTACTTTGAATTCATT+TGG	+	chr3_2:82439501-82439520	Msa0430550:intron	25.0%
!	ATTGTTCCTATACTATAACA+AGG	-	chr3_2:82439680-82439699	None:intergenic	25.0%
!!	ATGTTATTTATGTATGGTTG+GGG	+	chr3_2:82439733-82439752	Msa0430550:intron	25.0%
!!!	TCATAAGTTTTTTTCAACCT+CGG	-	chr3_2:82440390-82440409	None:intergenic	25.0%
!	TAATGAAATACTTCATGACA+TGG	-	chr3_2:82440467-82440486	None:intergenic	25.0%
!	TCATGTTATTGTTGTATTAC+TGG	+	chr3_2:82440504-82440523	Msa0430550:intron	25.0%
!!!	AATGTTTCTGGTTTATGAAA+TGG	+	chr3_2:82440664-82440683	Msa0430550:three_prime_UTR	25.0%
!	TAGTAGAGAAGAGAAAATTA+TGG	+	chr3_2:82439084-82439103	Msa0430550:exon	25.0%
!	CATGCATTATACACATAAAT+TGG	-	chr3_2:82439274-82439293	None:intergenic	25.0%
!	TATGTATATGTAGAGAGATA+CGG	+	chr3_2:82439319-82439338	Msa0430550:intron	25.0%
!	CACATTTATAAACACTATAG+AGG	-	chr3_2:82439460-82439479	None:intergenic	25.0%
!	TGAATTCAAAGTAAGTTCTT+TGG	-	chr3_2:82439499-82439518	None:intergenic	25.0%
!	GAACTTACTTTGAATTCATT+TGG	+	chr3_2:82439501-82439520	Msa0430550:intron	25.0%
!	ATTGTTCCTATACTATAACA+AGG	-	chr3_2:82439680-82439699	None:intergenic	25.0%
!!	ATGTTATTTATGTATGGTTG+GGG	+	chr3_2:82439733-82439752	Msa0430550:intron	25.0%
!!!	TCATAAGTTTTTTTCAACCT+CGG	-	chr3_2:82440390-82440409	None:intergenic	25.0%
!	TAATGAAATACTTCATGACA+TGG	-	chr3_2:82440467-82440486	None:intergenic	25.0%
!	TCATGTTATTGTTGTATTAC+TGG	+	chr3_2:82440504-82440523	Msa0430550:intron	25.0%
!!!	AATGTTTCTGGTTTATGAAA+TGG	+	chr3_2:82440664-82440683	Msa0430550:three_prime_UTR	25.0%
	CTTTGAATTCATTTGGACTT+TGG	+	chr3_2:82439508-82439527	Msa0430550:intron	30.0%
!	TGTTGATAAAAAGTTTGACC+TGG	+	chr3_2:82439569-82439588	Msa0430550:intron	30.0%
!	CAAACTCCTTGTTATAGTAT+AGG	+	chr3_2:82439671-82439690	Msa0430550:intron	30.0%
!	ATCAAACCAAGAATCTTTTC+GGG	-	chr3_2:82440541-82440560	None:intergenic	30.0%
!	CATCAAACCAAGAATCTTTT+CGG	-	chr3_2:82440542-82440561	None:intergenic	30.0%
	TGTTGAAAATATTACAAGGG+AGG	+	chr3_2:82440564-82440583	Msa0430550:CDS	30.0%
	TTTCTTTCACTGAATGTTTC+TGG	+	chr3_2:82440652-82440671	Msa0430550:three_prime_UTR	30.0%
	AAAGATGAGGATAAAGAGAA+TGG	+	chr3_2:82440802-82440821	Msa0430550:three_prime_UTR	30.0%
!!	AATCATAATTTGCTCTTTGG+CGG	-	chr3_2:82440984-82441003	None:intergenic	30.0%
!!	ACCAATCATAATTTGCTCTT+TGG	-	chr3_2:82440987-82441006	None:intergenic	30.0%
	AAGAGCAAATTATGATTGGT+AGG	+	chr3_2:82440987-82441006	Msa0430550:three_prime_UTR	30.0%
	CTTTGAATTCATTTGGACTT+TGG	+	chr3_2:82439508-82439527	Msa0430550:intron	30.0%
!	TGTTGATAAAAAGTTTGACC+TGG	+	chr3_2:82439569-82439588	Msa0430550:intron	30.0%
!	CAAACTCCTTGTTATAGTAT+AGG	+	chr3_2:82439671-82439690	Msa0430550:intron	30.0%
!	ATCAAACCAAGAATCTTTTC+GGG	-	chr3_2:82440541-82440560	None:intergenic	30.0%
!	CATCAAACCAAGAATCTTTT+CGG	-	chr3_2:82440542-82440561	None:intergenic	30.0%
	TGTTGAAAATATTACAAGGG+AGG	+	chr3_2:82440564-82440583	Msa0430550:CDS	30.0%
	TTTCTTTCACTGAATGTTTC+TGG	+	chr3_2:82440652-82440671	Msa0430550:three_prime_UTR	30.0%
	AAAGATGAGGATAAAGAGAA+TGG	+	chr3_2:82440802-82440821	Msa0430550:three_prime_UTR	30.0%
!!	AATCATAATTTGCTCTTTGG+CGG	-	chr3_2:82440984-82441003	None:intergenic	30.0%
!!	ACCAATCATAATTTGCTCTT+TGG	-	chr3_2:82440987-82441006	None:intergenic	30.0%
	AAGAGCAAATTATGATTGGT+AGG	+	chr3_2:82440987-82441006	Msa0430550:three_prime_UTR	30.0%
	TTCTAAATCCGAGAGATAAG+TGG	-	chr3_2:82439167-82439186	None:intergenic	35.0%
!	GTTTGTTGGAAATTCTGCTT+CGG	+	chr3_2:82439374-82439393	Msa0430550:intron	35.0%
	AACAAGGAGTTTGTGAAACT+AGG	-	chr3_2:82439664-82439683	None:intergenic	35.0%
	AACAGCTAGTGAAGCAAAAA+CGG	+	chr3_2:82439767-82439786	Msa0430550:CDS	35.0%
	AAACAAAGACAAGTTTGACC+TGG	+	chr3_2:82439809-82439828	Msa0430550:CDS	35.0%
!	TTATGATGATAGGTGCTACA+TGG	+	chr3_2:82440404-82440423	Msa0430550:intron	35.0%
	TATTGTTGTATTACTGGCAG+AGG	+	chr3_2:82440510-82440529	Msa0430550:intron	35.0%
	TAAAGGACTAGAAAATTGCC+CGG	-	chr3_2:82440606-82440625	None:intergenic	35.0%
	ACATTTACAGACCAAACAAC+CGG	-	chr3_2:82440865-82440884	None:intergenic	35.0%
!!!	TTCATGACTTCCGTTTTTTC+TGG	+	chr3_2:82440941-82440960	Msa0430550:three_prime_UTR	35.0%
!	GGAGGTTTTTCCAGAAAAAA+CGG	-	chr3_2:82440954-82440973	None:intergenic	35.0%
	GCCAAAGAGCAAATTATGAT+TGG	+	chr3_2:82440983-82441002	Msa0430550:three_prime_UTR	35.0%
	TTCTAAATCCGAGAGATAAG+TGG	-	chr3_2:82439167-82439186	None:intergenic	35.0%
!	GTTTGTTGGAAATTCTGCTT+CGG	+	chr3_2:82439374-82439393	Msa0430550:intron	35.0%
	AACAAGGAGTTTGTGAAACT+AGG	-	chr3_2:82439664-82439683	None:intergenic	35.0%
	AACAGCTAGTGAAGCAAAAA+CGG	+	chr3_2:82439767-82439786	Msa0430550:CDS	35.0%
	AAACAAAGACAAGTTTGACC+TGG	+	chr3_2:82439809-82439828	Msa0430550:CDS	35.0%
!	TTATGATGATAGGTGCTACA+TGG	+	chr3_2:82440404-82440423	Msa0430550:intron	35.0%
	TATTGTTGTATTACTGGCAG+AGG	+	chr3_2:82440510-82440529	Msa0430550:intron	35.0%
	TAAAGGACTAGAAAATTGCC+CGG	-	chr3_2:82440606-82440625	None:intergenic	35.0%
	ACATTTACAGACCAAACAAC+CGG	-	chr3_2:82440865-82440884	None:intergenic	35.0%
!!!	TTCATGACTTCCGTTTTTTC+TGG	+	chr3_2:82440941-82440960	Msa0430550:three_prime_UTR	35.0%
!	GGAGGTTTTTCCAGAAAAAA+CGG	-	chr3_2:82440954-82440973	None:intergenic	35.0%
	GCCAAAGAGCAAATTATGAT+TGG	+	chr3_2:82440983-82441002	Msa0430550:three_prime_UTR	35.0%
	AAAAGAAAAGAAGCAGACCG+TGG	-	chr3_2:82439213-82439232	None:intergenic	40.0%
	AATTAACCACCTCAAAGCCA+AGG	-	chr3_2:82439414-82439433	None:intergenic	40.0%
	GAGGTCATATCTAAGCAATG+TGG	-	chr3_2:82439441-82439460	None:intergenic	40.0%
	ATACCATAACAGCGATTACC+AGG	-	chr3_2:82439590-82439609	None:intergenic	40.0%
	GATCAAAGACTCCTAAGTCA+TGG	+	chr3_2:82439629-82439648	Msa0430550:intron	40.0%
	GGTCAAACATACCATGACTT+AGG	-	chr3_2:82439643-82439662	None:intergenic	40.0%
	ACTTAAGCTGCTTGAACTTG+TGG	+	chr3_2:82439866-82439885	Msa0430550:CDS	40.0%
	TGAACTTGTGGAACTTGAGA+CGG	+	chr3_2:82439878-82439897	Msa0430550:CDS	40.0%
	GAACTGAACTTACTTACGAC+CGG	-	chr3_2:82439909-82439928	None:intergenic	40.0%
	CGATAATGAGCTAGTGATGA+AGG	+	chr3_2:82440052-82440071	Msa0430550:CDS	40.0%
!	TTTAGCTCCTCTAATCTCAC+AGG	-	chr3_2:82440113-82440132	None:intergenic	40.0%
	TAGAGGAGCTAAAGACCATT+TGG	+	chr3_2:82440120-82440139	Msa0430550:CDS	40.0%
	CCATTTGGCAGCATGTAATT+CGG	+	chr3_2:82440135-82440154	Msa0430550:CDS	40.0%
	GCATGTAATTCGGAAGAAGA+AGG	+	chr3_2:82440145-82440164	Msa0430550:CDS	40.0%
	TAACAGCAGCAACAAACTTG+TGG	-	chr3_2:82440229-82440248	None:intergenic	40.0%
	GTTGCTGCTGTTAATCAGTT+AGG	+	chr3_2:82440236-82440255	Msa0430550:CDS	40.0%
	TCAAGCTCGAGGAAATTGAT+AGG	-	chr3_2:82440312-82440331	None:intergenic	40.0%
	ATAAAATTCGGTCAAGCTCG+AGG	-	chr3_2:82440323-82440342	None:intergenic	40.0%
	GTTGTACCCGAAAAGATTCT+TGG	+	chr3_2:82440532-82440551	Msa0430550:CDS	40.0%
	AAATATTACAAGGGAGGACG+TGG	+	chr3_2:82440570-82440589	Msa0430550:CDS	40.0%
!!	TGTGAAGAAAGTTAGCTCTG+TGG	+	chr3_2:82440713-82440732	Msa0430550:three_prime_UTR	40.0%
!!	GTGAAGAAAGTTAGCTCTGT+GGG	+	chr3_2:82440714-82440733	Msa0430550:three_prime_UTR	40.0%
	AAACCAACACGCTAGTAGTT+TGG	+	chr3_2:82440744-82440763	Msa0430550:three_prime_UTR	40.0%
	TCAGGATGAAGACAAAGATG+AGG	+	chr3_2:82440789-82440808	Msa0430550:three_prime_UTR	40.0%
	ATTTACAGACCAAACAACCG+GGG	-	chr3_2:82440863-82440882	None:intergenic	40.0%
	CATTTACAGACCAAACAACC+GGG	-	chr3_2:82440864-82440883	None:intergenic	40.0%
!!	GTAGGTTTTAATCTGCGATG+CGG	+	chr3_2:82441005-82441024	Msa0430550:three_prime_UTR	40.0%
	AAAAGAAAAGAAGCAGACCG+TGG	-	chr3_2:82439213-82439232	None:intergenic	40.0%
	AATTAACCACCTCAAAGCCA+AGG	-	chr3_2:82439414-82439433	None:intergenic	40.0%
	GAGGTCATATCTAAGCAATG+TGG	-	chr3_2:82439441-82439460	None:intergenic	40.0%
	ATACCATAACAGCGATTACC+AGG	-	chr3_2:82439590-82439609	None:intergenic	40.0%
	GATCAAAGACTCCTAAGTCA+TGG	+	chr3_2:82439629-82439648	Msa0430550:intron	40.0%
	GGTCAAACATACCATGACTT+AGG	-	chr3_2:82439643-82439662	None:intergenic	40.0%
	ACTTAAGCTGCTTGAACTTG+TGG	+	chr3_2:82439866-82439885	Msa0430550:CDS	40.0%
	TGAACTTGTGGAACTTGAGA+CGG	+	chr3_2:82439878-82439897	Msa0430550:CDS	40.0%
	GAACTGAACTTACTTACGAC+CGG	-	chr3_2:82439909-82439928	None:intergenic	40.0%
	CGATAATGAGCTAGTGATGA+AGG	+	chr3_2:82440052-82440071	Msa0430550:CDS	40.0%
!	TTTAGCTCCTCTAATCTCAC+AGG	-	chr3_2:82440113-82440132	None:intergenic	40.0%
	TAGAGGAGCTAAAGACCATT+TGG	+	chr3_2:82440120-82440139	Msa0430550:CDS	40.0%
	CCATTTGGCAGCATGTAATT+CGG	+	chr3_2:82440135-82440154	Msa0430550:CDS	40.0%
	GCATGTAATTCGGAAGAAGA+AGG	+	chr3_2:82440145-82440164	Msa0430550:CDS	40.0%
	TAACAGCAGCAACAAACTTG+TGG	-	chr3_2:82440229-82440248	None:intergenic	40.0%
	GTTGCTGCTGTTAATCAGTT+AGG	+	chr3_2:82440236-82440255	Msa0430550:CDS	40.0%
	TCAAGCTCGAGGAAATTGAT+AGG	-	chr3_2:82440312-82440331	None:intergenic	40.0%
	ATAAAATTCGGTCAAGCTCG+AGG	-	chr3_2:82440323-82440342	None:intergenic	40.0%
	GTTGTACCCGAAAAGATTCT+TGG	+	chr3_2:82440532-82440551	Msa0430550:CDS	40.0%
	AAATATTACAAGGGAGGACG+TGG	+	chr3_2:82440570-82440589	Msa0430550:CDS	40.0%
!!	TGTGAAGAAAGTTAGCTCTG+TGG	+	chr3_2:82440713-82440732	Msa0430550:three_prime_UTR	40.0%
!!	GTGAAGAAAGTTAGCTCTGT+GGG	+	chr3_2:82440714-82440733	Msa0430550:three_prime_UTR	40.0%
	AAACCAACACGCTAGTAGTT+TGG	+	chr3_2:82440744-82440763	Msa0430550:three_prime_UTR	40.0%
	TCAGGATGAAGACAAAGATG+AGG	+	chr3_2:82440789-82440808	Msa0430550:three_prime_UTR	40.0%
	ATTTACAGACCAAACAACCG+GGG	-	chr3_2:82440863-82440882	None:intergenic	40.0%
	CATTTACAGACCAAACAACC+GGG	-	chr3_2:82440864-82440883	None:intergenic	40.0%
!!	GTAGGTTTTAATCTGCGATG+CGG	+	chr3_2:82441005-82441024	Msa0430550:three_prime_UTR	40.0%
	TTATGGATGACTCCAGTGAC+CGG	+	chr3_2:82439101-82439120	Msa0430550:exon	45.0%
	CTCCGATCATGTCAATACCA+CGG	+	chr3_2:82439193-82439212	Msa0430550:CDS	45.0%
!!	GACCGTGGTATTGACATGAT+CGG	-	chr3_2:82439198-82439217	None:intergenic	45.0%
	TTGGAAATTCTGCTTCGGTC+CGG	+	chr3_2:82439379-82439398	Msa0430550:intron	45.0%
	TGACCTGGTAATCGCTGTTA+TGG	+	chr3_2:82439584-82439603	Msa0430550:intron	45.0%
	AGCTTAAGTCCATCCATGTC+AGG	-	chr3_2:82439855-82439874	None:intergenic	45.0%
	TGAACTTACTTACGACCGGA+AGG	-	chr3_2:82439905-82439924	None:intergenic	45.0%
	GTGATGAAGGCAGTTTACCA+TGG	+	chr3_2:82440065-82440084	Msa0430550:CDS	45.0%
	AGGTTTCACCAGAAACTCAG+AGG	-	chr3_2:82440093-82440112	None:intergenic	45.0%
	GGTGAAACCTGTGAGATTAG+AGG	+	chr3_2:82440103-82440122	Msa0430550:CDS	45.0%
	CCGAATTACATGCTGCCAAA+TGG	-	chr3_2:82440138-82440157	None:intergenic	45.0%
	TCGGAAGAAGAAGGACAATG+AGG	+	chr3_2:82440154-82440173	Msa0430550:CDS	45.0%
	AGAAGAAGTCCCAGTCTGTT+TGG	+	chr3_2:82440189-82440208	Msa0430550:CDS	45.0%
	ACTAGAAAATTGCCCGGAGA+TGG	-	chr3_2:82440600-82440619	None:intergenic	45.0%
	TTTACAGACCAAACAACCGG+GGG	-	chr3_2:82440862-82440881	None:intergenic	45.0%
	TTGCTCTTTGGCGGAATAGA+AGG	-	chr3_2:82440975-82440994	None:intergenic	45.0%
	TTATGGATGACTCCAGTGAC+CGG	+	chr3_2:82439101-82439120	Msa0430550:exon	45.0%
	CTCCGATCATGTCAATACCA+CGG	+	chr3_2:82439193-82439212	Msa0430550:CDS	45.0%
!!	GACCGTGGTATTGACATGAT+CGG	-	chr3_2:82439198-82439217	None:intergenic	45.0%
	TTGGAAATTCTGCTTCGGTC+CGG	+	chr3_2:82439379-82439398	Msa0430550:intron	45.0%
	TGACCTGGTAATCGCTGTTA+TGG	+	chr3_2:82439584-82439603	Msa0430550:intron	45.0%
	AGCTTAAGTCCATCCATGTC+AGG	-	chr3_2:82439855-82439874	None:intergenic	45.0%
	TGAACTTACTTACGACCGGA+AGG	-	chr3_2:82439905-82439924	None:intergenic	45.0%
	GTGATGAAGGCAGTTTACCA+TGG	+	chr3_2:82440065-82440084	Msa0430550:CDS	45.0%
	AGGTTTCACCAGAAACTCAG+AGG	-	chr3_2:82440093-82440112	None:intergenic	45.0%
	GGTGAAACCTGTGAGATTAG+AGG	+	chr3_2:82440103-82440122	Msa0430550:CDS	45.0%
	CCGAATTACATGCTGCCAAA+TGG	-	chr3_2:82440138-82440157	None:intergenic	45.0%
	TCGGAAGAAGAAGGACAATG+AGG	+	chr3_2:82440154-82440173	Msa0430550:CDS	45.0%
	AGAAGAAGTCCCAGTCTGTT+TGG	+	chr3_2:82440189-82440208	Msa0430550:CDS	45.0%
	ACTAGAAAATTGCCCGGAGA+TGG	-	chr3_2:82440600-82440619	None:intergenic	45.0%
	TTTACAGACCAAACAACCGG+GGG	-	chr3_2:82440862-82440881	None:intergenic	45.0%
	TTGCTCTTTGGCGGAATAGA+AGG	-	chr3_2:82440975-82440994	None:intergenic	45.0%
!!!	TAGTTTTTTTTTATTTTTAT+TGG	+	chr3_2:82440910-82440929	Msa0430550:three_prime_UTR	5.0%
!!!	TAGTTTTTTTTTATTTTTAT+TGG	+	chr3_2:82440910-82440929	Msa0430550:three_prime_UTR	5.0%
	ATAAGTGGAATCGCCGTCGA+CGG	-	chr3_2:82439152-82439171	None:intergenic	50.0%
	CGGCGATTCCACTTATCTCT+CGG	+	chr3_2:82439156-82439175	Msa0430550:CDS	50.0%
!!	CGTGGTATTGACATGATCGG+AGG	-	chr3_2:82439195-82439214	None:intergenic	50.0%
	AAAGCCAAGGCAGCAAATGC+CGG	-	chr3_2:82439401-82439420	None:intergenic	50.0%
!!	CATTTGCTGCCTTGGCTTTG+AGG	+	chr3_2:82439402-82439421	Msa0430550:intron	50.0%
	TAAGTCCATCCATGTCAGGC+AGG	-	chr3_2:82439851-82439870	None:intergenic	50.0%
	GGGACTTCTTCTGAGCTGAT+AGG	-	chr3_2:82440181-82440200	None:intergenic	50.0%
	CTCCACAGACCAAACAGACT+GGG	-	chr3_2:82440201-82440220	None:intergenic	50.0%
	TGTTTGTGTTGCGGACAGAG+TGG	-	chr3_2:82440279-82440298	None:intergenic	50.0%
!!	AAAGTTAGCTCTGTGGGCTG+TGG	+	chr3_2:82440720-82440739	Msa0430550:three_prime_UTR	50.0%
	CCACCAAACTACTAGCGTGT+TGG	-	chr3_2:82440750-82440769	None:intergenic	50.0%
	CCAACACGCTAGTAGTTTGG+TGG	+	chr3_2:82440747-82440766	Msa0430550:three_prime_UTR	50.0%
	ATCCTGATCTGCACTCCTTG+AGG	-	chr3_2:82440776-82440795	None:intergenic	50.0%
	CTCTTTGGCGGAATAGAAGG+AGG	-	chr3_2:82440972-82440991	None:intergenic	50.0%
	ATAAGTGGAATCGCCGTCGA+CGG	-	chr3_2:82439152-82439171	None:intergenic	50.0%
	CGGCGATTCCACTTATCTCT+CGG	+	chr3_2:82439156-82439175	Msa0430550:CDS	50.0%
!!	CGTGGTATTGACATGATCGG+AGG	-	chr3_2:82439195-82439214	None:intergenic	50.0%
	AAAGCCAAGGCAGCAAATGC+CGG	-	chr3_2:82439401-82439420	None:intergenic	50.0%
!!	CATTTGCTGCCTTGGCTTTG+AGG	+	chr3_2:82439402-82439421	Msa0430550:intron	50.0%
	TAAGTCCATCCATGTCAGGC+AGG	-	chr3_2:82439851-82439870	None:intergenic	50.0%
	GGGACTTCTTCTGAGCTGAT+AGG	-	chr3_2:82440181-82440200	None:intergenic	50.0%
	CTCCACAGACCAAACAGACT+GGG	-	chr3_2:82440201-82440220	None:intergenic	50.0%
	TGTTTGTGTTGCGGACAGAG+TGG	-	chr3_2:82440279-82440298	None:intergenic	50.0%
!!	AAAGTTAGCTCTGTGGGCTG+TGG	+	chr3_2:82440720-82440739	Msa0430550:three_prime_UTR	50.0%
	CCACCAAACTACTAGCGTGT+TGG	-	chr3_2:82440750-82440769	None:intergenic	50.0%
	CCAACACGCTAGTAGTTTGG+TGG	+	chr3_2:82440747-82440766	Msa0430550:three_prime_UTR	50.0%
	ATCCTGATCTGCACTCCTTG+AGG	-	chr3_2:82440776-82440795	None:intergenic	50.0%
	CTCTTTGGCGGAATAGAAGG+AGG	-	chr3_2:82440972-82440991	None:intergenic	50.0%
!	TCCAGTGACCGGTTTCCCAT+CGG	+	chr3_2:82439112-82439131	Msa0430550:CDS	55.0%
	CACGCATTCCGATGGGAAAC+CGG	-	chr3_2:82439123-82439142	None:intergenic	55.0%
	GCGAGAACACGCATTCCGAT+GGG	-	chr3_2:82439130-82439149	None:intergenic	55.0%
!!	TTGCTGCCTTGGCTTTGAGG+TGG	+	chr3_2:82439405-82439424	Msa0430550:intron	55.0%
	GGTGCACATCAGCGATTACC+AGG	-	chr3_2:82439830-82439849	None:intergenic	55.0%
	TGATGTGCACCTGCCTGACA+TGG	+	chr3_2:82439839-82439858	Msa0430550:CDS	55.0%
	GGAACTTGAGACGGACCTTC+CGG	+	chr3_2:82439887-82439906	Msa0430550:CDS	55.0%
!!	CCAGAAACTCAGAGGCACCA+TGG	-	chr3_2:82440085-82440104	None:intergenic	55.0%
	CCATGGTGCCTCTGAGTTTC+TGG	+	chr3_2:82440082-82440101	Msa0430550:CDS	55.0%
	GTCCCAGTCTGTTTGGTCTG+TGG	+	chr3_2:82440196-82440215	Msa0430550:CDS	55.0%
	GCTCCACAGACCAAACAGAC+TGG	-	chr3_2:82440202-82440221	None:intergenic	55.0%
!	GTAGTTTGGTGGCAGCCTCA+AGG	+	chr3_2:82440758-82440777	Msa0430550:three_prime_UTR	55.0%
!	AGCCTCAAGGAGTGCAGATC+AGG	+	chr3_2:82440771-82440790	Msa0430550:three_prime_UTR	55.0%
	GGGGCTTCTTCTGAGCTCAT+GGG	-	chr3_2:82440843-82440862	None:intergenic	55.0%
	ATGAGCTCAGAAGAAGCCCC+CGG	+	chr3_2:82440843-82440862	Msa0430550:three_prime_UTR	55.0%
	AGAAGAAGCCCCCGGTTGTT+TGG	+	chr3_2:82440851-82440870	Msa0430550:three_prime_UTR	55.0%
!	TCCAGTGACCGGTTTCCCAT+CGG	+	chr3_2:82439112-82439131	Msa0430550:CDS	55.0%
	CACGCATTCCGATGGGAAAC+CGG	-	chr3_2:82439123-82439142	None:intergenic	55.0%
	GCGAGAACACGCATTCCGAT+GGG	-	chr3_2:82439130-82439149	None:intergenic	55.0%
!!	TTGCTGCCTTGGCTTTGAGG+TGG	+	chr3_2:82439405-82439424	Msa0430550:intron	55.0%
	GGTGCACATCAGCGATTACC+AGG	-	chr3_2:82439830-82439849	None:intergenic	55.0%
	TGATGTGCACCTGCCTGACA+TGG	+	chr3_2:82439839-82439858	Msa0430550:CDS	55.0%
	GGAACTTGAGACGGACCTTC+CGG	+	chr3_2:82439887-82439906	Msa0430550:CDS	55.0%
!!	CCAGAAACTCAGAGGCACCA+TGG	-	chr3_2:82440085-82440104	None:intergenic	55.0%
	CCATGGTGCCTCTGAGTTTC+TGG	+	chr3_2:82440082-82440101	Msa0430550:CDS	55.0%
	GTCCCAGTCTGTTTGGTCTG+TGG	+	chr3_2:82440196-82440215	Msa0430550:CDS	55.0%
	GCTCCACAGACCAAACAGAC+TGG	-	chr3_2:82440202-82440221	None:intergenic	55.0%
!	GTAGTTTGGTGGCAGCCTCA+AGG	+	chr3_2:82440758-82440777	Msa0430550:three_prime_UTR	55.0%
!	AGCCTCAAGGAGTGCAGATC+AGG	+	chr3_2:82440771-82440790	Msa0430550:three_prime_UTR	55.0%
	GGGGCTTCTTCTGAGCTCAT+GGG	-	chr3_2:82440843-82440862	None:intergenic	55.0%
	ATGAGCTCAGAAGAAGCCCC+CGG	+	chr3_2:82440843-82440862	Msa0430550:three_prime_UTR	55.0%
	AGAAGAAGCCCCCGGTTGTT+TGG	+	chr3_2:82440851-82440870	Msa0430550:three_prime_UTR	55.0%
	TCCGATGGGAAACCGGTCAC+TGG	-	chr3_2:82439116-82439135	None:intergenic	60.0%
	GGCGAGAACACGCATTCCGA+TGG	-	chr3_2:82439131-82439150	None:intergenic	60.0%
	ATGCGTGTTCTCGCCGTCGA+CGG	+	chr3_2:82439136-82439155	Msa0430550:CDS	60.0%
	GTGCACCTGCCTGACATGGA+TGG	+	chr3_2:82439843-82439862	Msa0430550:CDS	60.0%
	AGGACGTGGCAAGCCATCTC+CGG	+	chr3_2:82440584-82440603	Msa0430550:CDS	60.0%
	GGGGGCTTCTTCTGAGCTCA+TGG	-	chr3_2:82440844-82440863	None:intergenic	60.0%
	TCCGATGGGAAACCGGTCAC+TGG	-	chr3_2:82439116-82439135	None:intergenic	60.0%
	GGCGAGAACACGCATTCCGA+TGG	-	chr3_2:82439131-82439150	None:intergenic	60.0%
	ATGCGTGTTCTCGCCGTCGA+CGG	+	chr3_2:82439136-82439155	Msa0430550:CDS	60.0%
	GTGCACCTGCCTGACATGGA+TGG	+	chr3_2:82439843-82439862	Msa0430550:CDS	60.0%
	AGGACGTGGCAAGCCATCTC+CGG	+	chr3_2:82440584-82440603	Msa0430550:CDS	60.0%
	GGGGGCTTCTTCTGAGCTCA+TGG	-	chr3_2:82440844-82440863	None:intergenic	60.0%
!	CGGTCCGGCATTTGCTGCCT+TGG	+	chr3_2:82439394-82439413	Msa0430550:intron	65.0%
	GGACGTGGCAAGCCATCTCC+GGG	+	chr3_2:82440585-82440604	Msa0430550:CDS	65.0%
!	CGGTCCGGCATTTGCTGCCT+TGG	+	chr3_2:82439394-82439413	Msa0430550:intron	65.0%
	GGACGTGGCAAGCCATCTCC+GGG	+	chr3_2:82440585-82440604	Msa0430550:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	82439056	82441090	82439056	ID=Msa0430550;Name=Msa0430550
chr3_2	mRNA	82439056	82441090	82439056	ID=Msa0430550-mRNA-1;Parent=Msa0430550;Name=Msa0430550-mRNA-1;_AED=0.28;_eAED=0.28;_QI=47\|1\|1\|1\|1\|1\|4\|472\|194
chr3_2	exon	82439056	82439214	82439056	ID=Msa0430550-mRNA-1:exon:22861;Parent=Msa0430550-mRNA-1
chr3_2	exon	82439763	82439915	82439763	ID=Msa0430550-mRNA-1:exon:22862;Parent=Msa0430550-mRNA-1
chr3_2	exon	82440036	82440266	82440036	ID=Msa0430550-mRNA-1:exon:22863;Parent=Msa0430550-mRNA-1
chr3_2	exon	82440530	82441090	82440530	ID=Msa0430550-mRNA-1:exon:22864;Parent=Msa0430550-mRNA-1
chr3_2	five_prime_UTR	82439056	82439102	82439056	ID=Msa0430550-mRNA-1:five_prime_utr;Parent=Msa0430550-mRNA-1
chr3_2	CDS	82439103	82439214	82439103	ID=Msa0430550-mRNA-1:cds;Parent=Msa0430550-mRNA-1
chr3_2	CDS	82439763	82439915	82439763	ID=Msa0430550-mRNA-1:cds;Parent=Msa0430550-mRNA-1
chr3_2	CDS	82440036	82440266	82440036	ID=Msa0430550-mRNA-1:cds;Parent=Msa0430550-mRNA-1
chr3_2	CDS	82440530	82440618	82440530	ID=Msa0430550-mRNA-1:cds;Parent=Msa0430550-mRNA-1
chr3_2	three_prime_UTR	82440619	82441090	82440619	ID=Msa0430550-mRNA-1:three_prime_utr;Parent=Msa0430550-mRNA-1

Gene Sequence

>Msa0430550

ATGGATGACTCCAGTGACCGGTTTCCCATCGGAATGCGTGTTCTCGCCGTCGACGGCGATTCCACTTATCTCTCGGATTTAGAAACTCGCCTCCGATCATGTCAATACCACGTAACAACAGCTAGTGAAGCAAAAACGGCATTGACTATGTTGCGAGAAAACAAAGACAAGTTTGACCTGGTAATCGCTGATGTGCACCTGCCTGACATGGATGGACTTAAGCTGCTTGAACTTGTGGAACTTGAGACGGACCTTCCGGTCGTAATGTTGTCTGAATCTAGCGATAATGAGCTAGTGATGAAGGCAGTTTACCATGGTGCCTCTGAGTTTCTGGTGAAACCTGTGAGATTAGAGGAGCTAAAGACCATTTGGCAGCATGTAATTCGGAAGAAGAAGGACAATGAGGACCTATCAGCTCAGAAGAAGTCCCAGTCTGTTTGGTCTGTGGAGCTGCACCACAAGTTTGTTGCTGCTGTTAATCAGTTAGGCATTGACAAGGTTGTACCCGAAAAGATTCTTGGTTTGATGAATGTTGAAAATATTACAAGGGAGGACGTGGCAAGCCATCTCCGGGCAATTTTCTAG

Protein sequence

>Msa0430550

MDDSSDRFPIGMRVLAVDGDSTYLSDLETRLRSCQYHVTTASEAKTALTMLRENKDKFDLVIADVHLPDMDGLKLLELVELETDLPVVMLSESSDNELVMKAVYHGASEFLVKPVRLEELKTIWQHVIRKKKDNEDLSAQKKSQSVWSVELHHKFVAAVNQLGIDKVVPEKILGLMNVENITREDVASHLRAIF*