AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0439340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0439340 MtrunA17_Chr3g0101111 95.726 117 5 0 1 117 168 284 1.26e-79 236
Msa0439340 MtrunA17_Chr3g0101091 94.872 117 6 0 1 117 168 284 1.79e-78 233
Msa0439340 MtrunA17_Chr3g0101081 94.872 117 6 0 1 117 168 284 1.79e-78 233
Msa0439340 MtrunA17_Chr7g0225201 89.256 121 13 0 1 121 169 289 3.31e-76 228
Msa0439340 MtrunA17_Chr3g0132681 88.430 121 14 0 1 121 169 289 2.30e-75 226
Msa0439340 MtrunA17_Chr5g0445271 84.615 117 18 0 1 117 169 285 7.69e-72 217
Msa0439340 MtrunA17_Chr5g0447731 73.846 130 34 0 1 130 165 294 1.70e-69 211
Msa0439340 MtrunA17_Chr7g0273851 69.828 116 34 1 2 117 167 281 3.69e-50 169
Msa0439340 MtrunA17_Chr1g0190951 66.949 118 38 1 2 119 167 283 7.92e-49 166
Msa0439340 MtrunA17_Chr7g0244601 63.793 116 42 0 2 117 171 286 3.94e-48 162
Msa0439340 MtrunA17_Chr1g0205471 68.644 118 35 2 2 119 167 282 1.28e-46 159
Msa0439340 MtrunA17_Chr5g0432541 64.103 117 42 0 1 117 169 285 1.82e-46 158
Msa0439340 MtrunA17_Chr3g0129771 60.630 127 49 1 2 128 176 301 7.06e-46 157
Msa0439340 MtrunA17_Chr8g0384001 55.906 127 55 1 2 128 171 296 2.47e-43 150
Msa0439340 MtrunA17_Chr8g0346511 69.167 120 36 1 2 121 181 299 2.01e-42 148
Msa0439340 MtrunA17_Chr6g0455931 65.254 118 41 0 1 118 171 288 8.84e-41 142
Msa0439340 MtrunA17_Chr3g0116811 66.667 117 39 0 2 118 169 285 1.14e-40 142
Msa0439340 MtrunA17_Chr4g0013051 66.379 116 39 0 2 117 167 282 1.53e-40 142
Msa0439340 MtrunA17_Chr3g0119911 65.574 122 41 1 1 122 162 282 1.88e-40 142
Msa0439340 MtrunA17_Chr1g0202831 35.115 131 81 3 2 129 550 679 1.30e-17 77.8
Msa0439340 MtrunA17_Chr3g0130151 35.036 137 84 4 2 134 383 518 1.49e-17 77.8
Msa0439340 MtrunA17_Chr8g0384301 34.074 135 85 3 2 133 438 571 1.44e-15 72.0
Msa0439340 MtrunA17_Chr4g0025041 37.037 135 78 6 2 132 134 265 2.34e-15 71.2
Msa0439340 MtrunA17_Chr8g0386981 35.294 119 73 3 2 117 547 664 1.58e-14 69.3
Msa0439340 MtrunA17_Chr4g0051191 36.667 120 69 5 2 117 301 417 1.58e-13 66.2
Msa0439340 MtrunA17_Chr2g0310781 35.294 136 78 4 1 132 991 1120 2.85e-13 65.5
Msa0439340 MtrunA17_Chr4g0041201 35.398 113 67 3 2 112 684 792 5.44e-13 64.7
Msa0439340 MtrunA17_Chr5g0424501 34.167 120 72 5 2 117 329 445 9.66e-13 63.9
Msa0439340 MtrunA17_Chr2g0299991 31.852 135 71 5 2 119 206 336 1.24e-12 63.5
Msa0439340 MtrunA17_Chr5g0406251 29.565 115 78 3 1 113 429 542 4.00e-12 62.0
Msa0439340 MtrunA17_Chr3g0115171 36.885 122 66 5 2 117 420 536 8.38e-12 61.2
Msa0439340 MtrunA17_Chr5g0397931 33.582 134 82 5 2 132 560 689 1.07e-11 60.8
Msa0439340 MtrunA17_Chr3g0133541 33.333 129 74 4 1 120 446 571 1.11e-11 60.8
Msa0439340 MtrunA17_Chr1g0196631 37.168 113 66 3 3 111 1048 1159 1.99e-11 60.5
Msa0439340 MtrunA17_Chr7g0260191 33.898 118 74 3 2 117 143 258 2.24e-11 60.1
Msa0439340 MtrunA17_Chr5g0419441 31.967 122 74 5 2 117 375 493 2.30e-11 60.1
Msa0439340 MtrunA17_Chr1g0179331 34.211 114 71 4 2 112 694 806 2.41e-11 60.1
Msa0439340 MtrunA17_Chr4g0025671 34.454 119 74 3 2 118 147 263 2.41e-11 60.1
Msa0439340 MtrunA17_Chr1g0185221 33.898 118 74 3 2 117 143 258 3.25e-11 59.7
Msa0439340 MtrunA17_Chr6g0449691 31.008 129 84 4 3 127 223 350 3.27e-11 59.7
Msa0439340 MtrunA17_Chr5g0427651 34.677 124 70 5 2 115 785 907 4.30e-11 59.3
Msa0439340 MtrunA17_Chr4g0065211 30.469 128 84 3 1 124 1267 1393 5.49e-11 58.9
Msa0439340 MtrunA17_Chr1g0173921 32.203 118 76 3 2 117 143 258 9.30e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0439340 AT5G55560.1 78.632 117 25 0 1 117 173 289 3.44e-66 203
Msa0439340 AT3G48260.2 66.379 116 39 0 2 117 70 185 1.03e-50 166
Msa0439340 AT3G48260.1 66.379 116 39 0 2 117 165 280 5.41e-50 166
Msa0439340 AT3G04910.3 69.828 116 34 1 2 117 41 155 1.44e-49 166
Msa0439340 AT3G04910.2 69.828 116 34 1 2 117 144 258 5.57e-49 166
Msa0439340 AT3G04910.1 69.828 116 34 1 2 117 167 281 6.49e-49 166
Msa0439340 AT3G51630.1 66.379 116 39 0 2 117 168 283 2.34e-46 157
Msa0439340 AT3G51630.2 66.379 116 39 0 2 117 168 283 2.34e-46 157
Msa0439340 AT3G18750.4 63.559 118 42 1 2 119 153 269 3.23e-46 157
Msa0439340 AT3G18750.1 63.559 118 42 1 2 119 171 287 6.33e-46 157
Msa0439340 AT3G18750.3 63.559 118 42 1 2 119 171 287 6.33e-46 157
Msa0439340 AT3G18750.5 63.559 118 42 1 2 119 171 287 6.33e-46 157
Msa0439340 AT5G28080.4 63.559 118 42 1 2 119 82 198 7.73e-46 153
Msa0439340 AT5G28080.3 63.559 118 42 1 2 119 82 198 7.73e-46 153
Msa0439340 AT5G28080.5 63.559 118 42 1 2 119 82 198 7.73e-46 153
Msa0439340 AT5G28080.6 63.559 118 42 1 2 119 82 198 7.73e-46 153
Msa0439340 AT5G28080.7 63.559 118 42 1 2 119 82 198 7.73e-46 153
Msa0439340 AT3G22420.1 62.931 116 42 1 2 117 167 281 8.56e-46 156
Msa0439340 AT5G28080.8 64.655 116 40 1 2 117 131 245 9.04e-46 154
Msa0439340 AT3G22420.5 62.931 116 42 1 2 117 167 281 9.30e-46 156
Msa0439340 AT1G49160.7 65.517 116 39 1 2 117 44 158 9.48e-46 154
Msa0439340 AT1G49160.6 65.517 116 39 1 2 117 44 158 9.48e-46 154
Msa0439340 AT1G49160.4 65.517 116 39 1 2 117 44 158 9.48e-46 154
Msa0439340 AT5G28080.2 64.655 116 40 1 2 117 168 282 1.15e-45 155
Msa0439340 AT5G58350.1 59.829 117 47 0 1 117 161 277 1.17e-45 156
Msa0439340 AT3G22420.4 62.931 116 42 1 2 117 238 352 1.35e-45 157
Msa0439340 AT3G22420.3 62.931 116 42 1 2 117 238 352 1.55e-45 157
Msa0439340 AT1G49160.1 65.517 116 39 1 2 117 153 267 3.28e-45 154
Msa0439340 AT1G49160.3 65.517 116 39 1 2 117 218 332 3.98e-45 153
Msa0439340 AT1G49160.2 65.517 116 39 1 2 117 171 285 9.14e-45 153
Msa0439340 AT1G49160.5 65.517 116 39 1 2 117 218 332 1.33e-44 154
Msa0439340 AT1G64630.1 61.069 131 50 1 2 132 159 288 1.57e-41 144
Msa0439340 AT3G22420.2 49.660 147 43 1 2 117 167 313 5.55e-41 144
Msa0439340 AT3G22420.6 49.660 147 43 1 2 117 167 313 6.18e-41 144
Msa0439340 AT5G41990.1 66.379 116 38 1 2 117 172 286 1.79e-40 142
Msa0439340 AT5G28080.1 61.538 91 35 0 29 119 1 91 8.43e-33 117
Msa0439340 AT1G53570.6 36.154 130 79 3 2 128 353 481 1.78e-18 80.9
Msa0439340 AT1G53570.7 36.154 130 79 3 2 128 353 481 1.78e-18 80.9
Msa0439340 AT1G53570.4 36.154 130 79 3 2 128 353 481 2.40e-18 80.5
Msa0439340 AT1G53570.1 36.154 130 79 3 2 128 353 481 2.49e-18 80.5
Msa0439340 AT1G53570.2 36.154 130 79 3 2 128 353 481 2.57e-18 80.5
Msa0439340 AT1G53570.3 36.154 130 79 3 2 128 353 481 2.57e-18 80.5
Msa0439340 AT4G08500.1 38.843 121 66 5 2 117 470 587 1.05e-16 75.9
Msa0439340 AT1G53570.5 35.115 131 74 4 8 128 326 455 6.06e-16 73.6
Msa0439340 AT1G63700.1 33.333 126 80 3 2 124 539 663 1.63e-13 66.6
Msa0439340 AT1G63700.2 33.333 126 80 3 2 124 539 663 1.63e-13 66.6
Msa0439340 AT4G08480.1 37.190 121 68 5 2 117 638 755 2.53e-13 66.2
Msa0439340 AT2G31010.1 35.965 114 67 3 2 113 656 765 3.42e-13 65.9
Msa0439340 AT2G31010.2 35.965 114 67 3 2 113 656 765 3.42e-13 65.9
Msa0439340 AT4G26890.1 38.333 120 66 5 1 117 139 253 4.86e-13 65.1
Msa0439340 AT2G42640.1 35.965 114 67 3 1 112 661 770 5.09e-13 65.1
Msa0439340 AT2G42640.4 35.965 114 67 3 1 112 623 732 5.26e-13 65.1
Msa0439340 AT3G20860.1 33.871 124 68 4 2 118 142 258 7.80e-13 64.7
Msa0439340 AT4G12020.5 37.302 126 62 7 2 119 1688 1804 1.88e-12 63.5
Msa0439340 AT4G12020.2 37.302 126 62 7 2 119 1763 1879 2.12e-12 63.5
Msa0439340 AT4G12020.4 37.302 126 62 7 2 119 1755 1871 2.14e-12 63.5
Msa0439340 AT5G55090.1 37.500 120 70 5 1 117 139 256 2.39e-12 63.2
Msa0439340 AT4G08470.3 35.484 124 72 5 2 120 358 478 3.50e-12 62.8
Msa0439340 AT5G55090.2 37.500 120 70 5 1 117 201 318 3.68e-12 62.8
Msa0439340 AT4G08470.1 35.484 124 72 5 2 120 440 560 4.28e-12 62.4
Msa0439340 AT5G28290.3 32.500 120 73 4 2 117 143 258 5.24e-12 62.4
Msa0439340 AT5G28290.1 32.500 120 73 4 2 117 143 258 5.85e-12 62.0
Msa0439340 AT5G28290.2 32.500 120 73 4 2 117 143 258 5.85e-12 62.0
Msa0439340 AT1G54510.4 34.167 120 71 4 2 117 143 258 6.97e-12 62.0
Msa0439340 AT1G54510.2 34.167 120 71 4 2 117 143 258 6.97e-12 62.0
Msa0439340 AT1G54510.1 34.167 120 71 4 2 117 143 258 6.97e-12 62.0
Msa0439340 AT1G54510.3 34.167 120 71 4 2 117 143 258 6.97e-12 62.0
Msa0439340 AT1G53165.1 32.500 120 75 4 2 117 150 267 9.18e-12 61.6
Msa0439340 AT1G53165.2 32.500 120 75 4 2 117 150 267 9.18e-12 61.6
Msa0439340 AT1G53165.3 32.500 120 75 4 2 117 150 267 9.19e-12 61.6
Msa0439340 AT3G63280.2 31.746 126 78 4 2 123 143 264 9.72e-12 61.6
Msa0439340 AT3G63280.3 31.746 126 78 4 2 123 143 264 9.72e-12 61.6
Msa0439340 AT3G63280.1 31.746 126 78 4 2 123 143 264 9.72e-12 61.6
Msa0439340 AT2G24360.1 32.456 114 74 3 2 113 270 382 1.16e-11 61.2
Msa0439340 AT3G63280.4 32.231 121 74 4 2 118 197 313 1.37e-11 61.2
Msa0439340 AT3G04810.2 31.667 120 74 4 2 117 143 258 1.64e-11 60.8
Msa0439340 AT3G04810.1 31.667 120 74 4 2 117 143 258 1.64e-11 60.8
Msa0439340 AT3G04810.3 31.667 120 74 4 2 117 143 258 1.64e-11 60.8
Msa0439340 AT1G16270.1 33.846 130 79 4 1 126 1009 1135 1.72e-11 60.8
Msa0439340 AT1G16270.2 33.846 130 79 4 1 126 1009 1135 1.72e-11 60.8
Msa0439340 AT1G16270.3 33.846 130 79 4 1 126 1009 1135 1.72e-11 60.8
Msa0439340 AT5G66850.2 33.071 127 71 4 1 117 485 607 2.58e-11 60.5
Msa0439340 AT5G66850.1 33.071 127 71 4 1 117 485 607 2.75e-11 60.1
Msa0439340 AT1G72180.1 33.077 130 66 6 3 113 829 956 4.10e-11 59.7
Msa0439340 AT1G69220.1 31.343 134 79 6 2 127 385 513 4.24e-11 59.7
Msa0439340 AT1G69220.2 31.343 134 79 6 2 127 358 486 4.61e-11 59.7
Msa0439340 AT2G32510.1 34.286 140 81 6 2 132 140 277 7.24e-11 58.9
Msa0439340 AT2G17700.1 30.435 115 77 3 1 113 420 533 8.04e-11 58.9

Find 24 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCAGCGCATTCAGTTCT+AGG 0.181449 3_3:-5302628 Msa0439340:CDS
TCTAGGCACACCGGAATTTA+TGG 0.201363 3_3:-5302611 Msa0439340:CDS
ACATTGTCACATTCACTATA+TGG 0.341582 3_3:+5302499 None:intergenic
AATGTATATTAGGTTAAGAT+TGG 0.380565 3_3:-5302685 Msa0439340:intron
ATGGTAGACATATACTCATT+TGG 0.414002 3_3:-5302556 Msa0439340:CDS
CTTAGGTTTGGCAGCAATTG+TGG 0.415298 3_3:-5302659 Msa0439340:CDS
ATATACAAGAAGGTGACTTC+AGG 0.446476 3_3:-5302469 Msa0439340:CDS
TAGGTTAAGATTGGTGACTT+AGG 0.448927 3_3:-5302676 Msa0439340:CDS
TAAGATTGGTGACTTAGGTT+TGG 0.458074 3_3:-5302671 Msa0439340:CDS
AGCTCTGGTGCCATAAATTC+CGG 0.461898 3_3:+5302601 None:intergenic
TAATCCTCTTCATATAGCTC+TGG 0.477445 3_3:+5302586 None:intergenic
TGAAGAGGATTACACAGAAA+TGG 0.479001 3_3:-5302575 Msa0439340:CDS
TCAGCAGAAGGTCTAGCCCT+TGG 0.491026 3_3:+5302373 None:intergenic
TTAGGTTTGGCAGCAATTGT+GGG 0.502201 3_3:-5302658 Msa0439340:CDS
TTTATCTTGTTCAAGGATTG+CGG 0.530284 3_3:+5302439 None:intergenic
TTCTGCTGAGGAATTGCTAA+AGG 0.536575 3_3:-5302359 Msa0439340:CDS
TGGAATGTTAGTATTAGAGA+TGG 0.547641 3_3:-5302536 Msa0439340:CDS
AAAAGTGTCTCGCTCAACCA+AGG 0.579858 3_3:-5302390 Msa0439340:CDS
TTTAGCAATTCCTCAGCAGA+AGG 0.580675 3_3:+5302361 None:intergenic
TGTTGCAAAGATATACAAGA+AGG 0.609288 3_3:-5302479 Msa0439340:CDS
GGCACCAGAGCTATATGAAG+AGG 0.616021 3_3:-5302590 Msa0439340:CDS
AAAGTGTCTCGCTCAACCAA+GGG 0.644670 3_3:-5302389 Msa0439340:CDS
AAGGGCTAGACCTTCTGCTG+AGG 0.666215 3_3:-5302371 Msa0439340:CDS
GCATTCAGTTCTAGGCACAC+CGG 0.687251 3_3:-5302620 Msa0439340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTGTTTTAATGTATATT+AGG - chr3_3:5303821-5303840 Msa0439340:intron 10.0%
!!! TATTTGTTTTAATGTATATT+AGG - chr3_3:5303821-5303840 Msa0439340:intron 10.0%
!!! AAGAGTTTTTGAATAATGAA+TGG + chr3_3:5302298-5302317 None:intergenic 20.0%
!! TACAGAACTATATTCAATTT+AGG - chr3_3:5302346-5302365 Msa0439340:CDS 20.0%
!! GAAATAAAAAACCTTGATTT+GGG + chr3_3:5302398-5302417 None:intergenic 20.0%
!! AGAAATAAAAAACCTTGATT+TGG + chr3_3:5302399-5302418 None:intergenic 20.0%
!! TATAACTTAATAGTTAGACT+TGG + chr3_3:5302583-5302602 None:intergenic 20.0%
!!! TTTAATATTTATAGCGGAAA+CGG - chr3_3:5302610-5302629 Msa0439340:CDS 20.0%
!!! AATCAACAGACATTTTTAAA+AGG + chr3_3:5302761-5302780 None:intergenic 20.0%
!!! TTTTGTTCTTTGAAAATCAA+AGG - chr3_3:5302779-5302798 Msa0439340:intron 20.0%
!! AATGTATATTAGGTTAAGAT+TGG - chr3_3:5303831-5303850 Msa0439340:intron 20.0%
!!! AAGAGTTTTTGAATAATGAA+TGG + chr3_3:5302298-5302317 None:intergenic 20.0%
!! TACAGAACTATATTCAATTT+AGG - chr3_3:5302346-5302365 Msa0439340:CDS 20.0%
!! GAAATAAAAAACCTTGATTT+GGG + chr3_3:5302398-5302417 None:intergenic 20.0%
!! AGAAATAAAAAACCTTGATT+TGG + chr3_3:5302399-5302418 None:intergenic 20.0%
!! TATAACTTAATAGTTAGACT+TGG + chr3_3:5302583-5302602 None:intergenic 20.0%
!!! TTTAATATTTATAGCGGAAA+CGG - chr3_3:5302610-5302629 Msa0439340:CDS 20.0%
!!! AATCAACAGACATTTTTAAA+AGG + chr3_3:5302761-5302780 None:intergenic 20.0%
!!! TTTTGTTCTTTGAAAATCAA+AGG - chr3_3:5302779-5302798 Msa0439340:intron 20.0%
!! AATGTATATTAGGTTAAGAT+TGG - chr3_3:5303831-5303850 Msa0439340:intron 20.0%
! TAATTCGTTTATTGTTGAAG+AGG - chr3_3:5302862-5302881 Msa0439340:intron 25.0%
! GATCAAAAGTAGTTTAATTG+TGG + chr3_3:5302889-5302908 None:intergenic 25.0%
!!! AAAGCATTTGATAATGAAGA+AGG - chr3_3:5303372-5303391 Msa0439340:intron 25.0%
!! ACATAAGCATGTTTCTATTA+AGG - chr3_3:5303608-5303627 Msa0439340:intron 25.0%
!! TACATCTTTTATCTTGTTCA+AGG + chr3_3:5304087-5304106 None:intergenic 25.0%
! TAATTCGTTTATTGTTGAAG+AGG - chr3_3:5302862-5302881 Msa0439340:intron 25.0%
! GATCAAAAGTAGTTTAATTG+TGG + chr3_3:5302889-5302908 None:intergenic 25.0%
!!! AAAGCATTTGATAATGAAGA+AGG - chr3_3:5303372-5303391 Msa0439340:intron 25.0%
!! ACATAAGCATGTTTCTATTA+AGG - chr3_3:5303608-5303627 Msa0439340:intron 25.0%
!! TACATCTTTTATCTTGTTCA+AGG + chr3_3:5304087-5304106 None:intergenic 25.0%
TCTCAATCAAACCTCAATTT+AGG + chr3_3:5302482-5302501 None:intergenic 30.0%
TATTAAACATATTACGCACG+CGG + chr3_3:5302642-5302661 None:intergenic 30.0%
TCTTGATCAAAGTATGACTA+TGG - chr3_3:5302687-5302706 Msa0439340:intron 30.0%
ATTTATATGTTGCGACACTA+GGG + chr3_3:5302731-5302750 None:intergenic 30.0%
AATTTATATGTTGCGACACT+AGG + chr3_3:5302732-5302751 None:intergenic 30.0%
TAGTGTCGCAACATATAAAT+TGG - chr3_3:5302731-5302750 Msa0439340:intron 30.0%
AGTGTCGCAACATATAAATT+GGG - chr3_3:5302732-5302751 Msa0439340:intron 30.0%
ATAACCTACTTATTGAGACT+TGG + chr3_3:5302812-5302831 None:intergenic 30.0%
!! TAAGATTTTAATAGTCGTCG+CGG - chr3_3:5302834-5302853 Msa0439340:intron 30.0%
!! TTTTTATGGCTCTACAACAT+AGG + chr3_3:5302920-5302939 None:intergenic 30.0%
TGTAGAGCCATAAAAATGTT+AGG - chr3_3:5302924-5302943 Msa0439340:intron 30.0%
! ATCGTGACCTAACATTTTTA+TGG + chr3_3:5302934-5302953 None:intergenic 30.0%
! AAATGTTTCTGAGTCTTTGT+CGG + chr3_3:5303277-5303296 None:intergenic 30.0%
! GAAGTGGTCTAAGAAAATTT+TGG - chr3_3:5303638-5303657 Msa0439340:intron 30.0%
! TGGTCTAAGAAAATTTTGGA+AGG - chr3_3:5303642-5303661 Msa0439340:intron 30.0%
!! GGTCTAAGAAAATTTTGGAA+GGG - chr3_3:5303643-5303662 Msa0439340:intron 30.0%
!! TGCAGTAATGTTTTTGTCAA+TGG - chr3_3:5303717-5303736 Msa0439340:intron 30.0%
! GTTTTTGTCAATGGAAATAC+TGG - chr3_3:5303726-5303745 Msa0439340:intron 30.0%
TGAAACAGAAATAATGTACC+TGG + chr3_3:5303752-5303771 None:intergenic 30.0%
ACATCTTGAGATAAACAACA+TGG + chr3_3:5303787-5303806 None:intergenic 30.0%
ATGGTAGACATATACTCATT+TGG - chr3_3:5303960-5303979 Msa0439340:intron 30.0%
TGGAATGTTAGTATTAGAGA+TGG - chr3_3:5303980-5303999 Msa0439340:intron 30.0%
ACATTGTCACATTCACTATA+TGG + chr3_3:5304020-5304039 None:intergenic 30.0%
TGTTGCAAAGATATACAAGA+AGG - chr3_3:5304037-5304056 Msa0439340:intron 30.0%
TTTATCTTGTTCAAGGATTG+CGG + chr3_3:5304080-5304099 None:intergenic 30.0%
GTACTCAACATCATCAAAAA+AGG + chr3_3:5304185-5304204 None:intergenic 30.0%
TCTCAATCAAACCTCAATTT+AGG + chr3_3:5302482-5302501 None:intergenic 30.0%
TATTAAACATATTACGCACG+CGG + chr3_3:5302642-5302661 None:intergenic 30.0%
TCTTGATCAAAGTATGACTA+TGG - chr3_3:5302687-5302706 Msa0439340:intron 30.0%
ATTTATATGTTGCGACACTA+GGG + chr3_3:5302731-5302750 None:intergenic 30.0%
AATTTATATGTTGCGACACT+AGG + chr3_3:5302732-5302751 None:intergenic 30.0%
TAGTGTCGCAACATATAAAT+TGG - chr3_3:5302731-5302750 Msa0439340:intron 30.0%
AGTGTCGCAACATATAAATT+GGG - chr3_3:5302732-5302751 Msa0439340:intron 30.0%
ATAACCTACTTATTGAGACT+TGG + chr3_3:5302812-5302831 None:intergenic 30.0%
!! TAAGATTTTAATAGTCGTCG+CGG - chr3_3:5302834-5302853 Msa0439340:intron 30.0%
!! TTTTTATGGCTCTACAACAT+AGG + chr3_3:5302920-5302939 None:intergenic 30.0%
TGTAGAGCCATAAAAATGTT+AGG - chr3_3:5302924-5302943 Msa0439340:intron 30.0%
! ATCGTGACCTAACATTTTTA+TGG + chr3_3:5302934-5302953 None:intergenic 30.0%
! AAATGTTTCTGAGTCTTTGT+CGG + chr3_3:5303277-5303296 None:intergenic 30.0%
! GAAGTGGTCTAAGAAAATTT+TGG - chr3_3:5303638-5303657 Msa0439340:intron 30.0%
! TGGTCTAAGAAAATTTTGGA+AGG - chr3_3:5303642-5303661 Msa0439340:intron 30.0%
!! GGTCTAAGAAAATTTTGGAA+GGG - chr3_3:5303643-5303662 Msa0439340:intron 30.0%
!! TGCAGTAATGTTTTTGTCAA+TGG - chr3_3:5303717-5303736 Msa0439340:intron 30.0%
! GTTTTTGTCAATGGAAATAC+TGG - chr3_3:5303726-5303745 Msa0439340:intron 30.0%
TGAAACAGAAATAATGTACC+TGG + chr3_3:5303752-5303771 None:intergenic 30.0%
ACATCTTGAGATAAACAACA+TGG + chr3_3:5303787-5303806 None:intergenic 30.0%
ATGGTAGACATATACTCATT+TGG - chr3_3:5303960-5303979 Msa0439340:intron 30.0%
TGGAATGTTAGTATTAGAGA+TGG - chr3_3:5303980-5303999 Msa0439340:intron 30.0%
ACATTGTCACATTCACTATA+TGG + chr3_3:5304020-5304039 None:intergenic 30.0%
TGTTGCAAAGATATACAAGA+AGG - chr3_3:5304037-5304056 Msa0439340:intron 30.0%
TTTATCTTGTTCAAGGATTG+CGG + chr3_3:5304080-5304099 None:intergenic 30.0%
GTACTCAACATCATCAAAAA+AGG + chr3_3:5304185-5304204 None:intergenic 30.0%
TAGTAACAAGACCCAAATCA+AGG - chr3_3:5302384-5302403 Msa0439340:CDS 35.0%
CAAACCTCAATTTAGGTCTA+GGG + chr3_3:5302475-5302494 None:intergenic 35.0%
TCAAACCTCAATTTAGGTCT+AGG + chr3_3:5302476-5302495 None:intergenic 35.0%
ATTTATATGTTGCGACACCA+GGG + chr3_3:5302514-5302533 None:intergenic 35.0%
AATTTATATGTTGCGACACC+AGG + chr3_3:5302515-5302534 None:intergenic 35.0%
!! TGGTGTCGCAACATATAAAT+TGG - chr3_3:5302514-5302533 Msa0439340:CDS 35.0%
! GGTGTCGCAACATATAAATT+GGG - chr3_3:5302515-5302534 Msa0439340:CDS 35.0%
CATACCAAGTCTCAATAAGT+AGG - chr3_3:5302805-5302824 Msa0439340:intron 35.0%
! GGCATCTAGAACAAAAAACA+TGG + chr3_3:5303234-5303253 None:intergenic 35.0%
!! GATGGATTTTGTTGAACACT+TGG + chr3_3:5303255-5303274 None:intergenic 35.0%
TTTGTTGAAACTGATCCAAC+CGG - chr3_3:5303294-5303313 Msa0439340:intron 35.0%
ATCGTATCTTCCATATCTTC+CGG + chr3_3:5303316-5303335 None:intergenic 35.0%
GGAAGATACGATGAATTACT+TGG - chr3_3:5303324-5303343 Msa0439340:intron 35.0%
AAGAAGGAATAGAAGTAGCA+TGG - chr3_3:5303388-5303407 Msa0439340:intron 35.0%
TCATTGCACTTTACAGTGTT+TGG - chr3_3:5303508-5303527 Msa0439340:intron 35.0%
TTTGTACAAGTGGGAATTTG+AGG - chr3_3:5303571-5303590 Msa0439340:intron 35.0%
TTGTACAAGTGGGAATTTGA+GGG - chr3_3:5303572-5303591 Msa0439340:intron 35.0%
!! CTATTAAGGCTTTGAAGAAG+TGG - chr3_3:5303622-5303641 Msa0439340:intron 35.0%
AGGTCTCTATGAATGATACA+AGG + chr3_3:5303693-5303712 None:intergenic 35.0%
CAAAAACATTACTGCAGTTG+AGG + chr3_3:5303713-5303732 None:intergenic 35.0%
! TAGGTTAAGATTGGTGACTT+AGG - chr3_3:5303840-5303859 Msa0439340:intron 35.0%
! TAAGATTGGTGACTTAGGTT+TGG - chr3_3:5303845-5303864 Msa0439340:intron 35.0%
TAATCCTCTTCATATAGCTC+TGG + chr3_3:5303933-5303952 None:intergenic 35.0%
TGAAGAGGATTACACAGAAA+TGG - chr3_3:5303941-5303960 Msa0439340:intron 35.0%
ATATACAAGAAGGTGACTTC+AGG - chr3_3:5304047-5304066 Msa0439340:intron 35.0%
TAGTAACAAGACCCAAATCA+AGG - chr3_3:5302384-5302403 Msa0439340:CDS 35.0%
CAAACCTCAATTTAGGTCTA+GGG + chr3_3:5302475-5302494 None:intergenic 35.0%
TCAAACCTCAATTTAGGTCT+AGG + chr3_3:5302476-5302495 None:intergenic 35.0%
ATTTATATGTTGCGACACCA+GGG + chr3_3:5302514-5302533 None:intergenic 35.0%
AATTTATATGTTGCGACACC+AGG + chr3_3:5302515-5302534 None:intergenic 35.0%
!! TGGTGTCGCAACATATAAAT+TGG - chr3_3:5302514-5302533 Msa0439340:CDS 35.0%
! GGTGTCGCAACATATAAATT+GGG - chr3_3:5302515-5302534 Msa0439340:CDS 35.0%
CATACCAAGTCTCAATAAGT+AGG - chr3_3:5302805-5302824 Msa0439340:intron 35.0%
! GGCATCTAGAACAAAAAACA+TGG + chr3_3:5303234-5303253 None:intergenic 35.0%
!! GATGGATTTTGTTGAACACT+TGG + chr3_3:5303255-5303274 None:intergenic 35.0%
TTTGTTGAAACTGATCCAAC+CGG - chr3_3:5303294-5303313 Msa0439340:intron 35.0%
ATCGTATCTTCCATATCTTC+CGG + chr3_3:5303316-5303335 None:intergenic 35.0%
GGAAGATACGATGAATTACT+TGG - chr3_3:5303324-5303343 Msa0439340:intron 35.0%
AAGAAGGAATAGAAGTAGCA+TGG - chr3_3:5303388-5303407 Msa0439340:intron 35.0%
TCATTGCACTTTACAGTGTT+TGG - chr3_3:5303508-5303527 Msa0439340:intron 35.0%
TTTGTACAAGTGGGAATTTG+AGG - chr3_3:5303571-5303590 Msa0439340:intron 35.0%
TTGTACAAGTGGGAATTTGA+GGG - chr3_3:5303572-5303591 Msa0439340:intron 35.0%
!! CTATTAAGGCTTTGAAGAAG+TGG - chr3_3:5303622-5303641 Msa0439340:intron 35.0%
AGGTCTCTATGAATGATACA+AGG + chr3_3:5303693-5303712 None:intergenic 35.0%
CAAAAACATTACTGCAGTTG+AGG + chr3_3:5303713-5303732 None:intergenic 35.0%
! TAGGTTAAGATTGGTGACTT+AGG - chr3_3:5303840-5303859 Msa0439340:intron 35.0%
! TAAGATTGGTGACTTAGGTT+TGG - chr3_3:5303845-5303864 Msa0439340:intron 35.0%
TAATCCTCTTCATATAGCTC+TGG + chr3_3:5303933-5303952 None:intergenic 35.0%
TGAAGAGGATTACACAGAAA+TGG - chr3_3:5303941-5303960 Msa0439340:intron 35.0%
ATATACAAGAAGGTGACTTC+AGG - chr3_3:5304047-5304066 Msa0439340:intron 35.0%
TGAACCCTAGACCTAAATTG+AGG - chr3_3:5302468-5302487 Msa0439340:CDS 40.0%
GATTGAGACATCAAAAACCC+TGG - chr3_3:5302494-5302513 Msa0439340:CDS 40.0%
! ATTTATAGCGGAAACGGTAG+TGG - chr3_3:5302616-5302635 Msa0439340:CDS 40.0%
TATCTTCCATATCTTCCGGT+TGG + chr3_3:5303312-5303331 None:intergenic 40.0%
TACGATGAATTACTTGGAGC+TGG - chr3_3:5303330-5303349 Msa0439340:intron 40.0%
TTACAGTGTTTGGAGAGACA+AGG - chr3_3:5303518-5303537 Msa0439340:intron 40.0%
CACACTGAATTTCATCACAG+AGG - chr3_3:5303548-5303567 Msa0439340:intron 40.0%
ATCACAGAGGTTTGTACAAG+TGG - chr3_3:5303561-5303580 Msa0439340:intron 40.0%
TCACAGAGGTTTGTACAAGT+GGG - chr3_3:5303562-5303581 Msa0439340:intron 40.0%
TTAGGTTTGGCAGCAATTGT+GGG - chr3_3:5303858-5303877 Msa0439340:intron 40.0%
TTTAGCAATTCCTCAGCAGA+AGG + chr3_3:5304158-5304177 None:intergenic 40.0%
TTCTGCTGAGGAATTGCTAA+AGG - chr3_3:5304157-5304176 Msa0439340:intron 40.0%
TGAACCCTAGACCTAAATTG+AGG - chr3_3:5302468-5302487 Msa0439340:CDS 40.0%
GATTGAGACATCAAAAACCC+TGG - chr3_3:5302494-5302513 Msa0439340:CDS 40.0%
! ATTTATAGCGGAAACGGTAG+TGG - chr3_3:5302616-5302635 Msa0439340:CDS 40.0%
TATCTTCCATATCTTCCGGT+TGG + chr3_3:5303312-5303331 None:intergenic 40.0%
TACGATGAATTACTTGGAGC+TGG - chr3_3:5303330-5303349 Msa0439340:intron 40.0%
TTACAGTGTTTGGAGAGACA+AGG - chr3_3:5303518-5303537 Msa0439340:intron 40.0%
CACACTGAATTTCATCACAG+AGG - chr3_3:5303548-5303567 Msa0439340:intron 40.0%
ATCACAGAGGTTTGTACAAG+TGG - chr3_3:5303561-5303580 Msa0439340:intron 40.0%
TCACAGAGGTTTGTACAAGT+GGG - chr3_3:5303562-5303581 Msa0439340:intron 40.0%
TTAGGTTTGGCAGCAATTGT+GGG - chr3_3:5303858-5303877 Msa0439340:intron 40.0%
TTTAGCAATTCCTCAGCAGA+AGG + chr3_3:5304158-5304177 None:intergenic 40.0%
TTCTGCTGAGGAATTGCTAA+AGG - chr3_3:5304157-5304176 Msa0439340:intron 40.0%
GTTTCTGAGTCTTTGTCGGA+TGG + chr3_3:5303273-5303292 None:intergenic 45.0%
ACTGATCCAACCGGAAGATA+TGG - chr3_3:5303303-5303322 Msa0439340:intron 45.0%
! GTGGGAATTTGAGGGAGTAT+AGG - chr3_3:5303580-5303599 Msa0439340:intron 45.0%
TGTCAATGGAAATACTGGCC+AGG - chr3_3:5303731-5303750 Msa0439340:intron 45.0%
CTTAGGTTTGGCAGCAATTG+TGG - chr3_3:5303857-5303876 Msa0439340:intron 45.0%
CATTCAGCGCATTCAGTTCT+AGG - chr3_3:5303888-5303907 Msa0439340:intron 45.0%
TCTAGGCACACCGGAATTTA+TGG - chr3_3:5303905-5303924 Msa0439340:intron 45.0%
!! AGCTCTGGTGCCATAAATTC+CGG + chr3_3:5303918-5303937 None:intergenic 45.0%
!! AAAAGTGTCTCGCTCAACCA+AGG - chr3_3:5304126-5304145 Msa0439340:intron 45.0%
! AAAGTGTCTCGCTCAACCAA+GGG - chr3_3:5304127-5304146 Msa0439340:intron 45.0%
GTTTCTGAGTCTTTGTCGGA+TGG + chr3_3:5303273-5303292 None:intergenic 45.0%
ACTGATCCAACCGGAAGATA+TGG - chr3_3:5303303-5303322 Msa0439340:intron 45.0%
! GTGGGAATTTGAGGGAGTAT+AGG - chr3_3:5303580-5303599 Msa0439340:intron 45.0%
TGTCAATGGAAATACTGGCC+AGG - chr3_3:5303731-5303750 Msa0439340:intron 45.0%
CTTAGGTTTGGCAGCAATTG+TGG - chr3_3:5303857-5303876 Msa0439340:intron 45.0%
CATTCAGCGCATTCAGTTCT+AGG - chr3_3:5303888-5303907 Msa0439340:intron 45.0%
TCTAGGCACACCGGAATTTA+TGG - chr3_3:5303905-5303924 Msa0439340:intron 45.0%
!! AGCTCTGGTGCCATAAATTC+CGG + chr3_3:5303918-5303937 None:intergenic 45.0%
!! AAAAGTGTCTCGCTCAACCA+AGG - chr3_3:5304126-5304145 Msa0439340:intron 45.0%
! AAAGTGTCTCGCTCAACCAA+GGG - chr3_3:5304127-5304146 Msa0439340:intron 45.0%
!!! TAATATTTTAATATTTATAG+CGG - chr3_3:5302604-5302623 Msa0439340:CDS 5.0%
!!! ATTATTATTATTATACTTTT+TGG - chr3_3:5303170-5303189 Msa0439340:intron 5.0%
!!! TAATATTTTAATATTTATAG+CGG - chr3_3:5302604-5302623 Msa0439340:CDS 5.0%
!!! ATTATTATTATTATACTTTT+TGG - chr3_3:5303170-5303189 Msa0439340:intron 5.0%
!! GCATTCAGTTCTAGGCACAC+CGG - chr3_3:5303896-5303915 Msa0439340:intron 50.0%
GGCACCAGAGCTATATGAAG+AGG - chr3_3:5303926-5303945 Msa0439340:intron 50.0%
!! GCATTCAGTTCTAGGCACAC+CGG - chr3_3:5303896-5303915 Msa0439340:intron 50.0%
GGCACCAGAGCTATATGAAG+AGG - chr3_3:5303926-5303945 Msa0439340:intron 50.0%
TCAGCAGAAGGTCTAGCCCT+TGG + chr3_3:5304146-5304165 None:intergenic 55.0%
AAGGGCTAGACCTTCTGCTG+AGG - chr3_3:5304145-5304164 Msa0439340:intron 55.0%
TCAGCAGAAGGTCTAGCCCT+TGG + chr3_3:5304146-5304165 None:intergenic 55.0%
AAGGGCTAGACCTTCTGCTG+AGG - chr3_3:5304145-5304164 Msa0439340:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 5302288 5304250 5302288 ID=Msa0439340;Name=Msa0439340
chr3_3 mRNA 5302288 5304250 5302288 ID=Msa0439340-mRNA-1;Parent=Msa0439340;Name=Msa0439340-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|0|0|0.5|1|1|2|0|136
chr3_3 exon 5304248 5304250 5304248 ID=Msa0439340-mRNA-1:exon:1114;Parent=Msa0439340-mRNA-1
chr3_3 exon 5302288 5302695 5302288 ID=Msa0439340-mRNA-1:exon:1113;Parent=Msa0439340-mRNA-1
chr3_3 CDS 5304248 5304250 5304248 ID=Msa0439340-mRNA-1:cds;Parent=Msa0439340-mRNA-1
chr3_3 CDS 5302288 5302695 5302288 ID=Msa0439340-mRNA-1:cds;Parent=Msa0439340-mRNA-1
Gene Sequence

>Msa0439340

ATGGTTAAGATTGGTGACTTAGGTTTGGCAGCAATTGTGGGAAAGAATCATTCAGCGCATTCAGTTCTAGGCACACCGGAATTTATGGCACCAGAGCTATATGAAGAGGATTACACAGAAATGGTAGACATATACTCATTTGGAATGTTAGTATTAGAGATGGTGACACTAGAGATTCCATATAGTGAATGTGACAATGTTGCAAAGATATACAAGAAGGTGACTTCAGGTGTGAGACCGCAATCCTTGAACAAGATAAAAGATGTAGAAGTGAAGACTTTCATCGAAAAGTGTCTCGCTCAACCAAGGGCTAGACCTTCTGCTGAGGAATTGCTAAAGGATCCTTTTTTGATGATGTTGAGTACGATGAAAACGACGATGTTGATTACTAATAGTTACTTTGTGATTTGA
Protein sequence

>Msa0439340

MVKIGDLGLAAIVGKNHSAHSVLGTPEFMAPELYEEDYTEMVDIYSFGMLVLEMVTLEIPYSECDNVAKIYKKVTSGVRPQSLNKIKDVEVKTFIEKCLAQPRARPSAEELLKDPFLMMLSTMKTTMLITNSYFVI*