AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0439520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0439520 MtrunA17_Chr3g0101091 96.899 129 4 0 2 130 169 297 4.96e-89 260
Msa0439520 MtrunA17_Chr3g0101081 96.899 129 4 0 2 130 169 297 4.96e-89 260
Msa0439520 MtrunA17_Chr3g0101111 96.875 128 4 0 2 129 169 296 1.70e-88 259
Msa0439520 MtrunA17_Chr7g0225201 89.844 128 13 0 2 129 170 297 1.74e-80 239
Msa0439520 MtrunA17_Chr3g0132681 88.095 126 15 0 2 127 170 295 1.09e-77 231
Msa0439520 MtrunA17_Chr5g0445271 86.207 116 16 0 2 117 170 285 7.84e-72 217
Msa0439520 MtrunA17_Chr5g0447731 77.236 123 28 0 2 124 166 288 1.99e-68 207
Msa0439520 MtrunA17_Chr7g0273851 68.548 124 36 2 2 123 167 289 1.07e-49 168
Msa0439520 MtrunA17_Chr1g0190951 66.935 124 38 2 2 123 167 289 2.77e-48 164
Msa0439520 MtrunA17_Chr7g0244601 59.836 122 49 0 2 123 171 292 2.45e-47 160
Msa0439520 MtrunA17_Chr1g0205471 67.742 124 36 3 2 123 167 288 4.69e-47 160
Msa0439520 MtrunA17_Chr5g0432541 63.793 116 42 0 2 117 170 285 1.21e-45 155
Msa0439520 MtrunA17_Chr3g0129771 63.248 117 42 1 2 118 176 291 2.56e-45 155
Msa0439520 MtrunA17_Chr8g0384001 60.000 115 45 1 2 116 171 284 1.51e-42 147
Msa0439520 MtrunA17_Chr8g0346511 69.828 116 34 1 2 117 181 295 1.14e-41 145
Msa0439520 MtrunA17_Chr3g0116811 66.379 116 39 0 2 117 169 284 1.15e-40 142
Msa0439520 MtrunA17_Chr4g0013051 63.115 122 45 0 2 123 167 288 2.36e-40 141
Msa0439520 MtrunA17_Chr6g0455931 65.517 116 40 0 2 117 172 287 2.56e-40 141
Msa0439520 MtrunA17_Chr3g0119911 67.241 116 37 1 2 117 163 277 3.29e-40 141
Msa0439520 MtrunA17_Chr3g0130151 36.364 121 73 3 2 119 383 502 2.20e-17 77.0
Msa0439520 MtrunA17_Chr1g0202831 38.983 118 68 3 2 116 550 666 2.37e-17 77.0
Msa0439520 MtrunA17_Chr8g0384301 35.537 121 74 3 2 119 438 557 4.54e-15 70.5
Msa0439520 MtrunA17_Chr8g0386981 35.593 118 72 3 2 116 547 663 1.70e-14 68.9
Msa0439520 MtrunA17_Chr4g0025041 37.190 121 70 5 3 120 135 252 4.46e-14 67.4
Msa0439520 MtrunA17_Chr4g0041201 34.959 123 72 4 2 122 684 800 2.69e-13 65.5
Msa0439520 MtrunA17_Chr2g0299991 31.724 145 75 6 2 126 206 346 3.79e-13 64.7
Msa0439520 MtrunA17_Chr8g0375141 35.156 128 70 6 2 121 181 303 4.44e-13 64.7
Msa0439520 MtrunA17_Chr4g0051191 35.833 120 70 5 2 117 301 417 7.52e-13 63.9
Msa0439520 MtrunA17_Chr4g0052981 34.646 127 70 6 2 120 142 263 1.36e-12 63.2
Msa0439520 MtrunA17_Chr5g0424501 34.454 119 71 5 2 116 329 444 1.58e-12 63.2
Msa0439520 MtrunA17_Chr2g0310781 36.522 115 68 3 1 111 991 1104 4.32e-12 62.0
Msa0439520 MtrunA17_Chr5g0406251 29.565 115 78 3 1 113 429 542 1.42e-11 60.5
Msa0439520 MtrunA17_Chr3g0115171 36.066 122 67 5 2 117 420 536 1.53e-11 60.5
Msa0439520 MtrunA17_Chr3g0133541 32.558 129 75 4 1 120 446 571 1.73e-11 60.1
Msa0439520 MtrunA17_Chr4g0025671 33.898 118 74 3 2 117 147 262 2.22e-11 60.1
Msa0439520 MtrunA17_Chr4g0052961 33.594 128 72 6 2 121 179 301 2.29e-11 59.7
Msa0439520 MtrunA17_Chr7g0260191 33.621 116 73 3 2 115 143 256 4.45e-11 58.9
Msa0439520 MtrunA17_Chr1g0196631 37.168 113 66 3 3 111 1048 1159 5.36e-11 58.9
Msa0439520 MtrunA17_Chr1g0179331 34.211 114 71 4 2 112 694 806 5.74e-11 58.5
Msa0439520 MtrunA17_Chr1g0185221 33.621 116 73 3 2 115 143 256 5.75e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0439520 AT5G55560.1 78.992 119 25 0 2 120 174 292 1.69e-67 206
Msa0439520 AT3G48260.2 65.517 116 40 0 2 117 70 185 4.43e-50 164
Msa0439520 AT3G48260.1 65.517 116 40 0 2 117 165 280 2.40e-49 164
Msa0439520 AT3G04910.3 67.742 124 37 2 2 123 41 163 1.98e-48 163
Msa0439520 AT3G04910.2 67.742 124 37 2 2 123 144 266 4.39e-48 163
Msa0439520 AT3G04910.1 67.742 124 37 2 2 123 167 289 5.78e-48 163
Msa0439520 AT1G49160.7 64.103 117 41 1 2 118 44 159 2.01e-45 152
Msa0439520 AT1G49160.6 64.103 117 41 1 2 118 44 159 2.01e-45 152
Msa0439520 AT1G49160.4 64.103 117 41 1 2 118 44 159 2.01e-45 152
Msa0439520 AT3G18750.4 63.793 116 41 1 2 117 153 267 3.02e-45 154
Msa0439520 AT3G18750.1 63.793 116 41 1 2 117 171 285 6.05e-45 154
Msa0439520 AT3G18750.3 63.793 116 41 1 2 117 171 285 6.05e-45 154
Msa0439520 AT3G18750.5 63.793 116 41 1 2 117 171 285 6.05e-45 154
Msa0439520 AT5G58350.1 59.483 116 47 0 2 117 162 277 6.21e-45 154
Msa0439520 AT5G28080.4 63.793 116 41 1 2 117 82 196 7.85e-45 150
Msa0439520 AT5G28080.3 63.793 116 41 1 2 117 82 196 7.85e-45 150
Msa0439520 AT5G28080.5 63.793 116 41 1 2 117 82 196 7.85e-45 150
Msa0439520 AT5G28080.6 63.793 116 41 1 2 117 82 196 7.85e-45 150
Msa0439520 AT5G28080.7 63.793 116 41 1 2 117 82 196 7.85e-45 150
Msa0439520 AT1G49160.1 64.103 117 41 1 2 118 153 268 9.23e-45 153
Msa0439520 AT3G51630.1 63.200 125 44 1 2 126 168 290 1.16e-44 152
Msa0439520 AT3G51630.2 63.200 125 44 1 2 126 168 290 1.16e-44 152
Msa0439520 AT1G49160.3 64.103 117 41 1 2 118 218 333 1.30e-44 152
Msa0439520 AT3G22420.1 61.864 118 44 1 2 119 167 283 1.47e-44 152
Msa0439520 AT1G49160.2 64.103 117 41 1 2 118 171 286 1.54e-44 152
Msa0439520 AT3G22420.4 61.864 118 44 1 2 119 238 354 1.90e-44 153
Msa0439520 AT5G28080.8 63.793 116 41 1 2 117 131 245 1.94e-44 150
Msa0439520 AT3G22420.5 61.864 118 44 1 2 119 167 283 2.27e-44 152
Msa0439520 AT3G22420.3 61.864 118 44 1 2 119 238 354 2.46e-44 153
Msa0439520 AT1G49160.5 64.103 117 41 1 2 118 218 333 3.41e-44 152
Msa0439520 AT5G28080.2 63.793 116 41 1 2 117 168 282 3.75e-44 150
Msa0439520 AT1G64630.1 67.241 116 37 1 2 117 159 273 6.57e-41 142
Msa0439520 AT5G41990.1 65.517 116 39 1 2 117 172 286 3.47e-40 141
Msa0439520 AT3G22420.6 48.366 153 48 1 2 123 167 319 8.50e-40 140
Msa0439520 AT3G22420.2 48.993 149 45 1 2 119 167 315 1.16e-39 140
Msa0439520 AT5G28080.1 61.798 89 34 0 29 117 1 89 7.04e-32 114
Msa0439520 AT1G53570.6 36.975 119 71 3 2 117 353 470 2.06e-17 77.4
Msa0439520 AT1G53570.7 36.975 119 71 3 2 117 353 470 2.09e-17 77.4
Msa0439520 AT1G53570.1 36.975 119 71 3 2 117 353 470 2.84e-17 77.0
Msa0439520 AT1G53570.4 36.975 119 71 3 2 117 353 470 2.90e-17 77.0
Msa0439520 AT1G53570.2 36.975 119 71 3 2 117 353 470 2.90e-17 77.0
Msa0439520 AT1G53570.3 36.975 119 71 3 2 117 353 470 2.90e-17 77.0
Msa0439520 AT4G26890.1 36.923 130 74 5 1 127 139 263 6.57e-15 70.1
Msa0439520 AT1G53570.5 35.833 120 66 4 8 117 326 444 7.79e-15 70.1
Msa0439520 AT1G63700.1 34.426 122 76 3 2 120 539 659 1.34e-13 66.6
Msa0439520 AT1G63700.2 34.426 122 76 3 2 120 539 659 1.34e-13 66.6
Msa0439520 AT2G31010.1 35.772 123 71 4 2 122 656 772 3.81e-13 65.5
Msa0439520 AT2G31010.2 35.772 123 71 4 2 122 656 772 3.81e-13 65.5
Msa0439520 AT3G20860.1 34.454 119 74 3 2 118 142 258 1.18e-12 63.9
Msa0439520 AT4G08480.1 37.500 120 67 5 2 116 638 754 2.30e-12 63.2
Msa0439520 AT2G42640.4 35.965 114 67 3 1 112 623 732 2.87e-12 62.8
Msa0439520 AT2G42640.1 35.965 114 67 3 1 112 661 770 4.50e-12 62.4
Msa0439520 AT2G24360.1 32.456 114 74 3 2 113 270 382 8.62e-12 61.2
Msa0439520 AT1G54510.4 33.898 118 70 4 2 115 143 256 1.15e-11 61.2
Msa0439520 AT1G54510.2 33.898 118 70 4 2 115 143 256 1.15e-11 61.2
Msa0439520 AT1G54510.1 33.898 118 70 4 2 115 143 256 1.15e-11 61.2
Msa0439520 AT1G54510.3 33.898 118 70 4 2 115 143 256 1.15e-11 61.2
Msa0439520 AT4G08470.3 35.833 120 69 5 2 116 358 474 1.23e-11 60.8
Msa0439520 AT4G12020.5 36.800 125 62 7 2 118 1688 1803 1.26e-11 61.2
Msa0439520 AT4G12020.4 36.800 125 62 7 2 118 1755 1870 1.27e-11 61.2
Msa0439520 AT4G12020.2 36.800 125 62 7 2 118 1763 1878 1.27e-11 61.2
Msa0439520 AT4G08470.1 35.833 120 69 5 2 116 440 556 1.39e-11 60.8
Msa0439520 AT3G63280.4 32.203 118 72 4 2 115 197 310 2.26e-11 60.1
Msa0439520 AT5G28290.1 31.667 120 74 4 2 117 143 258 2.28e-11 60.1
Msa0439520 AT5G28290.2 31.667 120 74 4 2 117 143 258 2.28e-11 60.1
Msa0439520 AT5G28290.3 31.667 120 74 4 2 117 143 258 2.36e-11 60.1
Msa0439520 AT3G63280.2 32.203 118 72 4 2 115 143 256 2.56e-11 60.1
Msa0439520 AT3G63280.3 32.203 118 72 4 2 115 143 256 2.56e-11 60.1
Msa0439520 AT3G63280.1 32.203 118 72 4 2 115 143 256 2.56e-11 60.1
Msa0439520 AT1G72180.1 32.609 138 70 7 3 121 829 962 2.57e-11 60.1
Msa0439520 AT2G17700.1 30.435 115 77 3 1 113 420 533 3.25e-11 59.7
Msa0439520 AT1G53165.3 31.967 122 77 4 2 119 150 269 4.84e-11 59.3
Msa0439520 AT1G53165.1 31.967 122 77 4 2 119 150 269 5.02e-11 59.3
Msa0439520 AT1G53165.2 31.967 122 77 4 2 119 150 269 5.07e-11 59.3
Msa0439520 AT4G29810.1 29.231 130 83 4 2 122 206 335 5.19e-11 58.9
Msa0439520 AT3G04810.2 30.833 120 75 4 2 117 143 258 7.87e-11 58.5
Msa0439520 AT3G04810.1 30.833 120 75 4 2 117 143 258 7.87e-11 58.5
Msa0439520 AT3G04810.3 30.833 120 75 4 2 117 143 258 7.87e-11 58.5
Msa0439520 AT1G69220.2 31.707 123 75 5 2 118 358 477 9.41e-11 58.5

Find 24 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCAGCGCATTCAGTTCT+AGG 0.181449 3_3:+5672137 Msa0439520:CDS
TCTAGGCACACCGGAATTTA+TGG 0.201363 3_3:+5672154 Msa0439520:CDS
ACATTGTCACATTCACTATA+TGG 0.341582 3_3:-5672266 None:intergenic
AATGTATATTAGGTTAAGAT+TGG 0.380565 3_3:+5672080 Msa0439520:intron
ATGGTAGACATATACTCATT+TGG 0.414002 3_3:+5672209 Msa0439520:CDS
CTTAGGTTTGGCAGCAATTG+TGG 0.415298 3_3:+5672106 Msa0439520:CDS
ATATACAAGAAGGTGACTTC+AGG 0.446476 3_3:+5672296 Msa0439520:CDS
TAGGTTAAGATTGGTGACTT+AGG 0.448927 3_3:+5672089 Msa0439520:intron
TAAGATTGGTGACTTAGGTT+TGG 0.458074 3_3:+5672094 Msa0439520:CDS
AGCTCTGGTGCCATAAATTC+CGG 0.461898 3_3:-5672164 None:intergenic
TAATCCTCTTCATATAGCTC+TGG 0.477445 3_3:-5672179 None:intergenic
TGAAGAGGATTACACAGAAA+TGG 0.479001 3_3:+5672190 Msa0439520:CDS
TCAGCAGAAGGTCTAGCCCT+TGG 0.491026 3_3:-5672392 None:intergenic
TTAGGTTTGGCAGCAATTGT+GGG 0.502201 3_3:+5672107 Msa0439520:CDS
TTTATCTTGTTCAAGGATTG+CGG 0.530284 3_3:-5672326 None:intergenic
TTCTGCTGAGGAATTGCTAA+AGG 0.536575 3_3:+5672406 Msa0439520:CDS
TGGAATGTTAGTATTAGAGA+TGG 0.547641 3_3:+5672229 Msa0439520:CDS
AAAAGTGTCTCGCTCAACCA+AGG 0.579858 3_3:+5672375 Msa0439520:CDS
TTTAGCAATTCCTCAGCAGA+AGG 0.580675 3_3:-5672404 None:intergenic
TGTTGCAAAGATATACAAGA+AGG 0.609288 3_3:+5672286 Msa0439520:CDS
GGCACCAGAGCTATATGAAG+AGG 0.616021 3_3:+5672175 Msa0439520:CDS
AAAGTGTCTCGCTCAACCAA+GGG 0.644670 3_3:+5672376 Msa0439520:CDS
AAGGGCTAGACCTTCTGCTG+AGG 0.666215 3_3:+5672394 Msa0439520:CDS
GCATTCAGTTCTAGGCACAC+CGG 0.687251 3_3:+5672145 Msa0439520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTGTTTTAATGTATATT+AGG + chr3_3:5672070-5672089 Msa0439520:intron 10.0%
!!! TATTTGTTTTAATGTATATT+AGG + chr3_3:5672070-5672089 Msa0439520:intron 10.0%
!!! AAGAGTTTTTGAATAATGAA+TGG - chr3_3:5670544-5670563 None:intergenic 20.0%
!! TACAGAACTATATTCAATTT+AGG + chr3_3:5670592-5670611 Msa0439520:intron 20.0%
!! GAAATAAAAAACCTTGATTT+GGG - chr3_3:5670644-5670663 None:intergenic 20.0%
!! AGAAATAAAAAACCTTGATT+TGG - chr3_3:5670645-5670664 None:intergenic 20.0%
!! TATAACTTAATAGTTAGACT+TGG - chr3_3:5670829-5670848 None:intergenic 20.0%
!!! TTTAATATTTATAGCGGAAA+CGG + chr3_3:5670856-5670875 Msa0439520:intron 20.0%
!!! AATCAACAGACATTTTTAAA+AGG - chr3_3:5671007-5671026 None:intergenic 20.0%
!!! TTTTGTTCTTTGAAAATCAA+AGG + chr3_3:5671025-5671044 Msa0439520:intron 20.0%
!! AATGTATATTAGGTTAAGAT+TGG + chr3_3:5672080-5672099 Msa0439520:intron 20.0%
!!! AAGAGTTTTTGAATAATGAA+TGG - chr3_3:5670544-5670563 None:intergenic 20.0%
!! TACAGAACTATATTCAATTT+AGG + chr3_3:5670592-5670611 Msa0439520:intron 20.0%
!! GAAATAAAAAACCTTGATTT+GGG - chr3_3:5670644-5670663 None:intergenic 20.0%
!! AGAAATAAAAAACCTTGATT+TGG - chr3_3:5670645-5670664 None:intergenic 20.0%
!! TATAACTTAATAGTTAGACT+TGG - chr3_3:5670829-5670848 None:intergenic 20.0%
!!! TTTAATATTTATAGCGGAAA+CGG + chr3_3:5670856-5670875 Msa0439520:intron 20.0%
!!! AATCAACAGACATTTTTAAA+AGG - chr3_3:5671007-5671026 None:intergenic 20.0%
!!! TTTTGTTCTTTGAAAATCAA+AGG + chr3_3:5671025-5671044 Msa0439520:intron 20.0%
!! AATGTATATTAGGTTAAGAT+TGG + chr3_3:5672080-5672099 Msa0439520:intron 20.0%
! TAATTCGTTTATTGTTGAAG+AGG + chr3_3:5671108-5671127 Msa0439520:intron 25.0%
! GATCAAAAGTAGTTTAATTG+TGG - chr3_3:5671135-5671154 None:intergenic 25.0%
!!! AAAGCATTTGATAATGAAGA+AGG + chr3_3:5671621-5671640 Msa0439520:intron 25.0%
!! ACATAAGCATGTTTCTATTA+AGG + chr3_3:5671857-5671876 Msa0439520:intron 25.0%
!! TACATCTTTTATCTTGTTCA+AGG - chr3_3:5672336-5672355 None:intergenic 25.0%
! TACTCAACATCATCAAAAAA+AGG - chr3_3:5672434-5672453 None:intergenic 25.0%
! TAATTCGTTTATTGTTGAAG+AGG + chr3_3:5671108-5671127 Msa0439520:intron 25.0%
! GATCAAAAGTAGTTTAATTG+TGG - chr3_3:5671135-5671154 None:intergenic 25.0%
!!! AAAGCATTTGATAATGAAGA+AGG + chr3_3:5671621-5671640 Msa0439520:intron 25.0%
!! ACATAAGCATGTTTCTATTA+AGG + chr3_3:5671857-5671876 Msa0439520:intron 25.0%
!! TACATCTTTTATCTTGTTCA+AGG - chr3_3:5672336-5672355 None:intergenic 25.0%
! TACTCAACATCATCAAAAAA+AGG - chr3_3:5672434-5672453 None:intergenic 25.0%
TCTCAATCAAACCTCAATTT+AGG - chr3_3:5670728-5670747 None:intergenic 30.0%
TATTAAACATATTACGCACG+CGG - chr3_3:5670888-5670907 None:intergenic 30.0%
TCTTGATCAAAGTATGACTA+TGG + chr3_3:5670933-5670952 Msa0439520:intron 30.0%
ATTTATATGTTGCGACACTA+GGG - chr3_3:5670977-5670996 None:intergenic 30.0%
AATTTATATGTTGCGACACT+AGG - chr3_3:5670978-5670997 None:intergenic 30.0%
TAGTGTCGCAACATATAAAT+TGG + chr3_3:5670977-5670996 Msa0439520:intron 30.0%
AGTGTCGCAACATATAAATT+GGG + chr3_3:5670978-5670997 Msa0439520:intron 30.0%
ATAACCTACTTATTGAGACT+TGG - chr3_3:5671058-5671077 None:intergenic 30.0%
!! TAAGATTTTAATAGTCGTCG+CGG + chr3_3:5671080-5671099 Msa0439520:intron 30.0%
!! TTTTTATGGCTCTACAACAT+AGG - chr3_3:5671166-5671185 None:intergenic 30.0%
TGTAGAGCCATAAAAATGTT+AGG + chr3_3:5671170-5671189 Msa0439520:intron 30.0%
! ATCGTGACCTAACATTTTTA+TGG - chr3_3:5671180-5671199 None:intergenic 30.0%
! AAATGTTTCTGAGTCTTTGT+CGG - chr3_3:5671526-5671545 None:intergenic 30.0%
TAAAGTCTTTCAATCATTGC+AGG - chr3_3:5671696-5671715 None:intergenic 30.0%
! GAAGTGGTCTAAGAAAATTT+TGG + chr3_3:5671887-5671906 Msa0439520:intron 30.0%
! TGGTCTAAGAAAATTTTGGA+AGG + chr3_3:5671891-5671910 Msa0439520:intron 30.0%
!! GGTCTAAGAAAATTTTGGAA+GGG + chr3_3:5671892-5671911 Msa0439520:intron 30.0%
!! TGCAGTAATGTTTTTGTCAA+TGG + chr3_3:5671966-5671985 Msa0439520:intron 30.0%
! GTTTTTGTCAATGGAAATAC+TGG + chr3_3:5671975-5671994 Msa0439520:intron 30.0%
TGAAACAGAAATAATGTACC+TGG - chr3_3:5672001-5672020 None:intergenic 30.0%
ACATCTTGAGATAAACAACA+TGG - chr3_3:5672036-5672055 None:intergenic 30.0%
ATGGTAGACATATACTCATT+TGG + chr3_3:5672209-5672228 Msa0439520:CDS 30.0%
TGGAATGTTAGTATTAGAGA+TGG + chr3_3:5672229-5672248 Msa0439520:CDS 30.0%
ACATTGTCACATTCACTATA+TGG - chr3_3:5672269-5672288 None:intergenic 30.0%
TGTTGCAAAGATATACAAGA+AGG + chr3_3:5672286-5672305 Msa0439520:CDS 30.0%
TTTATCTTGTTCAAGGATTG+CGG - chr3_3:5672329-5672348 None:intergenic 30.0%
TCTCAATCAAACCTCAATTT+AGG - chr3_3:5670728-5670747 None:intergenic 30.0%
TATTAAACATATTACGCACG+CGG - chr3_3:5670888-5670907 None:intergenic 30.0%
TCTTGATCAAAGTATGACTA+TGG + chr3_3:5670933-5670952 Msa0439520:intron 30.0%
ATTTATATGTTGCGACACTA+GGG - chr3_3:5670977-5670996 None:intergenic 30.0%
AATTTATATGTTGCGACACT+AGG - chr3_3:5670978-5670997 None:intergenic 30.0%
TAGTGTCGCAACATATAAAT+TGG + chr3_3:5670977-5670996 Msa0439520:intron 30.0%
AGTGTCGCAACATATAAATT+GGG + chr3_3:5670978-5670997 Msa0439520:intron 30.0%
ATAACCTACTTATTGAGACT+TGG - chr3_3:5671058-5671077 None:intergenic 30.0%
!! TAAGATTTTAATAGTCGTCG+CGG + chr3_3:5671080-5671099 Msa0439520:intron 30.0%
!! TTTTTATGGCTCTACAACAT+AGG - chr3_3:5671166-5671185 None:intergenic 30.0%
TGTAGAGCCATAAAAATGTT+AGG + chr3_3:5671170-5671189 Msa0439520:intron 30.0%
! ATCGTGACCTAACATTTTTA+TGG - chr3_3:5671180-5671199 None:intergenic 30.0%
! AAATGTTTCTGAGTCTTTGT+CGG - chr3_3:5671526-5671545 None:intergenic 30.0%
TAAAGTCTTTCAATCATTGC+AGG - chr3_3:5671696-5671715 None:intergenic 30.0%
! GAAGTGGTCTAAGAAAATTT+TGG + chr3_3:5671887-5671906 Msa0439520:intron 30.0%
! TGGTCTAAGAAAATTTTGGA+AGG + chr3_3:5671891-5671910 Msa0439520:intron 30.0%
!! GGTCTAAGAAAATTTTGGAA+GGG + chr3_3:5671892-5671911 Msa0439520:intron 30.0%
!! TGCAGTAATGTTTTTGTCAA+TGG + chr3_3:5671966-5671985 Msa0439520:intron 30.0%
! GTTTTTGTCAATGGAAATAC+TGG + chr3_3:5671975-5671994 Msa0439520:intron 30.0%
TGAAACAGAAATAATGTACC+TGG - chr3_3:5672001-5672020 None:intergenic 30.0%
ACATCTTGAGATAAACAACA+TGG - chr3_3:5672036-5672055 None:intergenic 30.0%
ATGGTAGACATATACTCATT+TGG + chr3_3:5672209-5672228 Msa0439520:CDS 30.0%
TGGAATGTTAGTATTAGAGA+TGG + chr3_3:5672229-5672248 Msa0439520:CDS 30.0%
ACATTGTCACATTCACTATA+TGG - chr3_3:5672269-5672288 None:intergenic 30.0%
TGTTGCAAAGATATACAAGA+AGG + chr3_3:5672286-5672305 Msa0439520:CDS 30.0%
TTTATCTTGTTCAAGGATTG+CGG - chr3_3:5672329-5672348 None:intergenic 30.0%
TAGTAACAAGACCCAAATCA+AGG + chr3_3:5670630-5670649 Msa0439520:intron 35.0%
CAAACCTCAATTTAGGTCTA+GGG - chr3_3:5670721-5670740 None:intergenic 35.0%
TCAAACCTCAATTTAGGTCT+AGG - chr3_3:5670722-5670741 None:intergenic 35.0%
ATTTATATGTTGCGACACCA+GGG - chr3_3:5670760-5670779 None:intergenic 35.0%
AATTTATATGTTGCGACACC+AGG - chr3_3:5670761-5670780 None:intergenic 35.0%
!! TGGTGTCGCAACATATAAAT+TGG + chr3_3:5670760-5670779 Msa0439520:intron 35.0%
! GGTGTCGCAACATATAAATT+GGG + chr3_3:5670761-5670780 Msa0439520:intron 35.0%
CATACCAAGTCTCAATAAGT+AGG + chr3_3:5671051-5671070 Msa0439520:intron 35.0%
! GGCATCTAGAACAAAAAACA+TGG - chr3_3:5671483-5671502 None:intergenic 35.0%
!! GATGGATTTTGTTGAACACT+TGG - chr3_3:5671504-5671523 None:intergenic 35.0%
TTTGTTGAAACTGATCCAAC+CGG + chr3_3:5671543-5671562 Msa0439520:intron 35.0%
ATCGTATCTTCCATATCTTC+CGG - chr3_3:5671565-5671584 None:intergenic 35.0%
GGAAGATACGATGAATTACT+TGG + chr3_3:5671573-5671592 Msa0439520:intron 35.0%
AAGAAGGAATAGAAGTAGCA+TGG + chr3_3:5671637-5671656 Msa0439520:intron 35.0%
TCATTGCACTTTACAGTGTT+TGG + chr3_3:5671757-5671776 Msa0439520:intron 35.0%
TTTGTACAAGTGGGAATTTG+AGG + chr3_3:5671820-5671839 Msa0439520:intron 35.0%
TTGTACAAGTGGGAATTTGA+GGG + chr3_3:5671821-5671840 Msa0439520:intron 35.0%
!! CTATTAAGGCTTTGAAGAAG+TGG + chr3_3:5671871-5671890 Msa0439520:intron 35.0%
AGGTCTCTATGAATGATACA+AGG - chr3_3:5671942-5671961 None:intergenic 35.0%
CAAAAACATTACTGCAGTTG+AGG - chr3_3:5671962-5671981 None:intergenic 35.0%
! TAGGTTAAGATTGGTGACTT+AGG + chr3_3:5672089-5672108 Msa0439520:intron 35.0%
! TAAGATTGGTGACTTAGGTT+TGG + chr3_3:5672094-5672113 Msa0439520:CDS 35.0%
TAATCCTCTTCATATAGCTC+TGG - chr3_3:5672182-5672201 None:intergenic 35.0%
TGAAGAGGATTACACAGAAA+TGG + chr3_3:5672190-5672209 Msa0439520:CDS 35.0%
ATATACAAGAAGGTGACTTC+AGG + chr3_3:5672296-5672315 Msa0439520:CDS 35.0%
TAGTAACAAGACCCAAATCA+AGG + chr3_3:5670630-5670649 Msa0439520:intron 35.0%
CAAACCTCAATTTAGGTCTA+GGG - chr3_3:5670721-5670740 None:intergenic 35.0%
TCAAACCTCAATTTAGGTCT+AGG - chr3_3:5670722-5670741 None:intergenic 35.0%
ATTTATATGTTGCGACACCA+GGG - chr3_3:5670760-5670779 None:intergenic 35.0%
AATTTATATGTTGCGACACC+AGG - chr3_3:5670761-5670780 None:intergenic 35.0%
!! TGGTGTCGCAACATATAAAT+TGG + chr3_3:5670760-5670779 Msa0439520:intron 35.0%
! GGTGTCGCAACATATAAATT+GGG + chr3_3:5670761-5670780 Msa0439520:intron 35.0%
CATACCAAGTCTCAATAAGT+AGG + chr3_3:5671051-5671070 Msa0439520:intron 35.0%
! GGCATCTAGAACAAAAAACA+TGG - chr3_3:5671483-5671502 None:intergenic 35.0%
!! GATGGATTTTGTTGAACACT+TGG - chr3_3:5671504-5671523 None:intergenic 35.0%
TTTGTTGAAACTGATCCAAC+CGG + chr3_3:5671543-5671562 Msa0439520:intron 35.0%
ATCGTATCTTCCATATCTTC+CGG - chr3_3:5671565-5671584 None:intergenic 35.0%
GGAAGATACGATGAATTACT+TGG + chr3_3:5671573-5671592 Msa0439520:intron 35.0%
AAGAAGGAATAGAAGTAGCA+TGG + chr3_3:5671637-5671656 Msa0439520:intron 35.0%
TCATTGCACTTTACAGTGTT+TGG + chr3_3:5671757-5671776 Msa0439520:intron 35.0%
TTTGTACAAGTGGGAATTTG+AGG + chr3_3:5671820-5671839 Msa0439520:intron 35.0%
TTGTACAAGTGGGAATTTGA+GGG + chr3_3:5671821-5671840 Msa0439520:intron 35.0%
!! CTATTAAGGCTTTGAAGAAG+TGG + chr3_3:5671871-5671890 Msa0439520:intron 35.0%
AGGTCTCTATGAATGATACA+AGG - chr3_3:5671942-5671961 None:intergenic 35.0%
CAAAAACATTACTGCAGTTG+AGG - chr3_3:5671962-5671981 None:intergenic 35.0%
! TAGGTTAAGATTGGTGACTT+AGG + chr3_3:5672089-5672108 Msa0439520:intron 35.0%
! TAAGATTGGTGACTTAGGTT+TGG + chr3_3:5672094-5672113 Msa0439520:CDS 35.0%
TAATCCTCTTCATATAGCTC+TGG - chr3_3:5672182-5672201 None:intergenic 35.0%
TGAAGAGGATTACACAGAAA+TGG + chr3_3:5672190-5672209 Msa0439520:CDS 35.0%
ATATACAAGAAGGTGACTTC+AGG + chr3_3:5672296-5672315 Msa0439520:CDS 35.0%
TGAACCCTAGACCTAAATTG+AGG + chr3_3:5670714-5670733 Msa0439520:intron 40.0%
GATTGAGACATCAAAAACCC+TGG + chr3_3:5670740-5670759 Msa0439520:intron 40.0%
! ATTTATAGCGGAAACGGTAG+TGG + chr3_3:5670862-5670881 Msa0439520:intron 40.0%
TATCTTCCATATCTTCCGGT+TGG - chr3_3:5671561-5671580 None:intergenic 40.0%
TACGATGAATTACTTGGAGC+TGG + chr3_3:5671579-5671598 Msa0439520:intron 40.0%
TTACAGTGTTTGGAGAGACA+AGG + chr3_3:5671767-5671786 Msa0439520:intron 40.0%
CACACTGAATTTCATCACAG+AGG + chr3_3:5671797-5671816 Msa0439520:intron 40.0%
ATCACAGAGGTTTGTACAAG+TGG + chr3_3:5671810-5671829 Msa0439520:intron 40.0%
TCACAGAGGTTTGTACAAGT+GGG + chr3_3:5671811-5671830 Msa0439520:intron 40.0%
TTAGGTTTGGCAGCAATTGT+GGG + chr3_3:5672107-5672126 Msa0439520:CDS 40.0%
TTTAGCAATTCCTCAGCAGA+AGG - chr3_3:5672407-5672426 None:intergenic 40.0%
TTCTGCTGAGGAATTGCTAA+AGG + chr3_3:5672406-5672425 Msa0439520:CDS 40.0%
TGAACCCTAGACCTAAATTG+AGG + chr3_3:5670714-5670733 Msa0439520:intron 40.0%
GATTGAGACATCAAAAACCC+TGG + chr3_3:5670740-5670759 Msa0439520:intron 40.0%
! ATTTATAGCGGAAACGGTAG+TGG + chr3_3:5670862-5670881 Msa0439520:intron 40.0%
TATCTTCCATATCTTCCGGT+TGG - chr3_3:5671561-5671580 None:intergenic 40.0%
TACGATGAATTACTTGGAGC+TGG + chr3_3:5671579-5671598 Msa0439520:intron 40.0%
TTACAGTGTTTGGAGAGACA+AGG + chr3_3:5671767-5671786 Msa0439520:intron 40.0%
CACACTGAATTTCATCACAG+AGG + chr3_3:5671797-5671816 Msa0439520:intron 40.0%
ATCACAGAGGTTTGTACAAG+TGG + chr3_3:5671810-5671829 Msa0439520:intron 40.0%
TCACAGAGGTTTGTACAAGT+GGG + chr3_3:5671811-5671830 Msa0439520:intron 40.0%
TTAGGTTTGGCAGCAATTGT+GGG + chr3_3:5672107-5672126 Msa0439520:CDS 40.0%
TTTAGCAATTCCTCAGCAGA+AGG - chr3_3:5672407-5672426 None:intergenic 40.0%
TTCTGCTGAGGAATTGCTAA+AGG + chr3_3:5672406-5672425 Msa0439520:CDS 40.0%
GTTTCTGAGTCTTTGTCGGA+TGG - chr3_3:5671522-5671541 None:intergenic 45.0%
ACTGATCCAACCGGAAGATA+TGG + chr3_3:5671552-5671571 Msa0439520:intron 45.0%
! GTGGGAATTTGAGGGAGTAT+AGG + chr3_3:5671829-5671848 Msa0439520:intron 45.0%
TGTCAATGGAAATACTGGCC+AGG + chr3_3:5671980-5671999 Msa0439520:intron 45.0%
CTTAGGTTTGGCAGCAATTG+TGG + chr3_3:5672106-5672125 Msa0439520:CDS 45.0%
CATTCAGCGCATTCAGTTCT+AGG + chr3_3:5672137-5672156 Msa0439520:CDS 45.0%
TCTAGGCACACCGGAATTTA+TGG + chr3_3:5672154-5672173 Msa0439520:CDS 45.0%
!! AGCTCTGGTGCCATAAATTC+CGG - chr3_3:5672167-5672186 None:intergenic 45.0%
!! AAAAGTGTCTCGCTCAACCA+AGG + chr3_3:5672375-5672394 Msa0439520:CDS 45.0%
! AAAGTGTCTCGCTCAACCAA+GGG + chr3_3:5672376-5672395 Msa0439520:CDS 45.0%
GTTTCTGAGTCTTTGTCGGA+TGG - chr3_3:5671522-5671541 None:intergenic 45.0%
ACTGATCCAACCGGAAGATA+TGG + chr3_3:5671552-5671571 Msa0439520:intron 45.0%
! GTGGGAATTTGAGGGAGTAT+AGG + chr3_3:5671829-5671848 Msa0439520:intron 45.0%
TGTCAATGGAAATACTGGCC+AGG + chr3_3:5671980-5671999 Msa0439520:intron 45.0%
CTTAGGTTTGGCAGCAATTG+TGG + chr3_3:5672106-5672125 Msa0439520:CDS 45.0%
CATTCAGCGCATTCAGTTCT+AGG + chr3_3:5672137-5672156 Msa0439520:CDS 45.0%
TCTAGGCACACCGGAATTTA+TGG + chr3_3:5672154-5672173 Msa0439520:CDS 45.0%
!! AGCTCTGGTGCCATAAATTC+CGG - chr3_3:5672167-5672186 None:intergenic 45.0%
!! AAAAGTGTCTCGCTCAACCA+AGG + chr3_3:5672375-5672394 Msa0439520:CDS 45.0%
! AAAGTGTCTCGCTCAACCAA+GGG + chr3_3:5672376-5672395 Msa0439520:CDS 45.0%
!!! TAATATTTTAATATTTATAG+CGG + chr3_3:5670850-5670869 Msa0439520:intron 5.0%
!!! ATTATTATTATTATACTTTT+TGG + chr3_3:5671419-5671438 Msa0439520:intron 5.0%
!!! TAATATTTTAATATTTATAG+CGG + chr3_3:5670850-5670869 Msa0439520:intron 5.0%
!!! ATTATTATTATTATACTTTT+TGG + chr3_3:5671419-5671438 Msa0439520:intron 5.0%
!! GCATTCAGTTCTAGGCACAC+CGG + chr3_3:5672145-5672164 Msa0439520:CDS 50.0%
GGCACCAGAGCTATATGAAG+AGG + chr3_3:5672175-5672194 Msa0439520:CDS 50.0%
!! GCATTCAGTTCTAGGCACAC+CGG + chr3_3:5672145-5672164 Msa0439520:CDS 50.0%
GGCACCAGAGCTATATGAAG+AGG + chr3_3:5672175-5672194 Msa0439520:CDS 50.0%
TCAGCAGAAGGTCTAGCCCT+TGG - chr3_3:5672395-5672414 None:intergenic 55.0%
AAGGGCTAGACCTTCTGCTG+AGG + chr3_3:5672394-5672413 Msa0439520:CDS 55.0%
TCAGCAGAAGGTCTAGCCCT+TGG - chr3_3:5672395-5672414 None:intergenic 55.0%
AAGGGCTAGACCTTCTGCTG+AGG + chr3_3:5672394-5672413 Msa0439520:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 5670534 5672481 5670534 ID=Msa0439520;Name=Msa0439520
chr3_3 mRNA 5670534 5672481 5670534 ID=Msa0439520-mRNA-1;Parent=Msa0439520;Name=Msa0439520-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|0|0|0.5|1|1|2|0|130
chr3_3 exon 5670534 5670536 5670534 ID=Msa0439520-mRNA-1:exon:1151;Parent=Msa0439520-mRNA-1
chr3_3 exon 5672092 5672481 5672092 ID=Msa0439520-mRNA-1:exon:1152;Parent=Msa0439520-mRNA-1
chr3_3 CDS 5670534 5670536 5670534 ID=Msa0439520-mRNA-1:cds;Parent=Msa0439520-mRNA-1
chr3_3 CDS 5672092 5672481 5672092 ID=Msa0439520-mRNA-1:cds;Parent=Msa0439520-mRNA-1
Gene Sequence

>Msa0439520

ATGGTTAAGATTGGTGACTTAGGTTTGGCAGCAATTGTGGGAAAGAATCATTCAGCGCATTCAGTTCTAGGCACACCGGAATTTATGGCACCAGAGCTATATGAAGAGGATTACACAGAAATGGTAGACATATACTCATTTGGAATGTTAGTATTAGAGATGGTGACACTAGAGATTCCATATAGTGAATGTGACAATGTTGCAAAGATATACAAGAAGGTGACTTCAGGTGTGAGACCGCAATCCTTGAACAAGATAAAAGATGTAGAAGTGAAGACTTTCATCGAAAAGTGTCTCGCTCAACCAAGGGCTAGACCTTCTGCTGAGGAATTGCTAAAGGATCCTTTTTTTGATGATGTTGAGTACGATGAAAACGACGATGTTGATTACTAA
Protein sequence

>Msa0439520

MVKIGDLGLAAIVGKNHSAHSVLGTPEFMAPELYEEDYTEMVDIYSFGMLVLEMVTLEIPYSECDNVAKIYKKVTSGVRPQSLNKIKDVEVKTFIEKCLAQPRARPSAEELLKDPFFDDVEYDENDDVDY*