Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0441030 | XP_003615230.1 | 80.851 | 94 | 17 | 1 | 1 | 94 | 1 | 93 | 2.86e-33 | 128 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0441030 | sp|Q9SB61|ZHD2_ARATH | 45.349 | 86 | 40 | 2 | 8 | 93 | 5 | 83 | 8.58e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0441030 | G7KBF3 | 80.851 | 94 | 17 | 1 | 1 | 94 | 1 | 93 | 1.37e-33 | 128 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0441030 | TF | zf-HD |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0441030 | MtrunA17_Chr5g0427051 | 80.851 | 94 | 17 | 1 | 1 | 94 | 1 | 93 | 2.63e-37 | 128 |
| Msa0441030 | MtrunA17_Chr3g0133181 | 53.012 | 83 | 30 | 2 | 20 | 94 | 34 | 115 | 1.37e-17 | 77.0 |
| Msa0441030 | MtrunA17_Chr7g0243651 | 58.929 | 56 | 21 | 1 | 57 | 112 | 1 | 54 | 3.79e-16 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0441030 | AT4G24660.1 | 45.349 | 86 | 40 | 2 | 8 | 93 | 5 | 83 | 8.72e-12 | 60.8 |
| Msa0441030 | AT4G24660.2 | 45.349 | 86 | 40 | 2 | 8 | 93 | 5 | 83 | 1.01e-11 | 60.8 |
| Msa0441030 | AT3G50890.1 | 67.568 | 37 | 12 | 0 | 57 | 93 | 58 | 94 | 5.20e-11 | 59.3 |
Find 26 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATCTCATCATAGAGAATT+TGG | 0.197551 | 3_3:+9258351 | None:intergenic |
| CATCATAGAGAATTTGGATT+TGG | 0.235332 | 3_3:+9258357 | None:intergenic |
| TGACGGTTGTTGCGAGTTTA+TGG | 0.243395 | 3_3:-9258530 | Msa0441030:CDS |
| CGTGATTCTTCTGACATTCT+TGG | 0.260175 | 3_3:+9258578 | None:intergenic |
| GCTTGGAGAATTCAGAATTC+TGG | 0.289840 | 3_3:-9258467 | Msa0441030:CDS |
| ATCATAGAGAATTTGGATTT+GGG | 0.356435 | 3_3:+9258358 | None:intergenic |
| TTCGGTGGCCACGCTGTTGA+CGG | 0.414354 | 3_3:-9258547 | Msa0441030:CDS |
| AAGCTGTTATTTGCGCTGCT+TGG | 0.453807 | 3_3:-9258484 | Msa0441030:CDS |
| CGAATCAGATTCGCTTATGT+TGG | 0.484764 | 3_3:-9258388 | Msa0441030:CDS |
| GTTTATGGCTGCCGGTGAAA+AGG | 0.487572 | 3_3:-9258515 | Msa0441030:CDS |
| AATGGAATTTGATGAACAAA+CGG | 0.488408 | 3_3:-9258755 | Msa0441030:CDS |
| AAGAATCACGCTGTTAGCTT+CGG | 0.499674 | 3_3:-9258565 | Msa0441030:CDS |
| AAACTCACCATCGTAGTTGA+CGG | 0.531063 | 3_3:-9258646 | Msa0441030:CDS |
| AACGGAACAAGAAGAGGAGA+TGG | 0.536127 | 3_3:-9258737 | Msa0441030:CDS |
| GAGTCATAACTCGTTGGCAC+TGG | 0.537593 | 3_3:+9258694 | None:intergenic |
| AATCACGCTGTTAGCTTCGG+TGG | 0.563601 | 3_3:-9258562 | Msa0441030:CDS |
| ATCGTAGTTGACGGAAGGAA+AGG | 0.583477 | 3_3:-9258637 | Msa0441030:CDS |
| TGAACAAACGGAACAAGAAG+AGG | 0.590353 | 3_3:-9258743 | Msa0441030:CDS |
| CCAACGAGTTATGACTCACT+TGG | 0.611073 | 3_3:-9258688 | Msa0441030:CDS |
| TGTTGCGAGTTTATGGCTGC+CGG | 0.619539 | 3_3:-9258523 | Msa0441030:CDS |
| TTTCCTTCCGTCAACTACGA+TGG | 0.621872 | 3_3:+9258639 | None:intergenic |
| TCACCATCGTAGTTGACGGA+AGG | 0.639038 | 3_3:-9258642 | Msa0441030:CDS |
| CCAAGTGAGTCATAACTCGT+TGG | 0.644449 | 3_3:+9258688 | None:intergenic |
| CGCAACAACCGTCAACAGCG+TGG | 0.700015 | 3_3:+9258539 | None:intergenic |
| GGAAGGAAAGGTAACGACAA+CGG | 0.717401 | 3_3:-9258625 | Msa0441030:CDS |
| AGGAAAGGTAACGACAACGG+CGG | 0.798323 | 3_3:-9258622 | Msa0441030:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCATAGAGAATTTGGATTT+GGG | + | chr3_3:9258723-9258742 | None:intergenic | 25.0% |
| ! | TAATCTCATCATAGAGAATT+TGG | + | chr3_3:9258730-9258749 | None:intergenic | 25.0% |
| CATCATAGAGAATTTGGATT+TGG | + | chr3_3:9258724-9258743 | None:intergenic | 30.0% | |
| AACAACACAAAAACAGGAAG+GGG | + | chr3_3:9258654-9258673 | None:intergenic | 35.0% | |
| CAACAACACAAAAACAGGAA+GGG | + | chr3_3:9258655-9258674 | None:intergenic | 35.0% | |
| ACAACAACACAAAAACAGGA+AGG | + | chr3_3:9258656-9258675 | None:intergenic | 35.0% | |
| !!! | TTCCTGTTTTTGTGTTGTTG+TGG | - | chr3_3:9258655-9258674 | Msa0441030:CDS | 35.0% |
| TGAACAAACGGAACAAGAAG+AGG | - | chr3_3:9258335-9258354 | Msa0441030:CDS | 40.0% | |
| AAACTCACCATCGTAGTTGA+CGG | - | chr3_3:9258432-9258451 | Msa0441030:CDS | 40.0% | |
| CGTGATTCTTCTGACATTCT+TGG | + | chr3_3:9258503-9258522 | None:intergenic | 40.0% | |
| ! | AAGAATCACGCTGTTAGCTT+CGG | - | chr3_3:9258513-9258532 | Msa0441030:CDS | 40.0% |
| ! | CTTCAAGTGTTCCTTTTCAC+CGG | + | chr3_3:9258577-9258596 | None:intergenic | 40.0% |
| GCTTGGAGAATTCAGAATTC+TGG | - | chr3_3:9258611-9258630 | Msa0441030:CDS | 40.0% | |
| AGGAAGGGGGAATAAGAATT+CGG | + | chr3_3:9258640-9258659 | None:intergenic | 40.0% | |
| ACAACACAAAAACAGGAAGG+GGG | + | chr3_3:9258653-9258672 | None:intergenic | 40.0% | |
| CACCACAACAACACAAAAAC+AGG | + | chr3_3:9258660-9258679 | None:intergenic | 40.0% | |
| !!! | CTGTTTTTGTGTTGTTGTGG+TGG | - | chr3_3:9258658-9258677 | Msa0441030:CDS | 40.0% |
| CGAATCAGATTCGCTTATGT+TGG | - | chr3_3:9258690-9258709 | Msa0441030:CDS | 40.0% | |
| AACGGAACAAGAAGAGGAGA+TGG | - | chr3_3:9258341-9258360 | Msa0441030:CDS | 45.0% | |
| !!! | GGCACTGGTGTTTGTGTTTT+TGG | + | chr3_3:9258372-9258391 | None:intergenic | 45.0% |
| CCAAGTGAGTCATAACTCGT+TGG | + | chr3_3:9258393-9258412 | None:intergenic | 45.0% | |
| CCAACGAGTTATGACTCACT+TGG | - | chr3_3:9258390-9258409 | Msa0441030:CDS | 45.0% | |
| TTTCCTTCCGTCAACTACGA+TGG | + | chr3_3:9258442-9258461 | None:intergenic | 45.0% | |
| ATCGTAGTTGACGGAAGGAA+AGG | - | chr3_3:9258441-9258460 | Msa0441030:CDS | 45.0% | |
| GGAAGGAAAGGTAACGACAA+CGG | - | chr3_3:9258453-9258472 | Msa0441030:CDS | 45.0% | |
| TGACGGTTGTTGCGAGTTTA+TGG | - | chr3_3:9258548-9258567 | Msa0441030:CDS | 45.0% | |
| ! | AAGCTGTTATTTGCGCTGCT+TGG | - | chr3_3:9258594-9258613 | Msa0441030:CDS | 45.0% |
| GAGTCATAACTCGTTGGCAC+TGG | + | chr3_3:9258387-9258406 | None:intergenic | 50.0% | |
| TCACCATCGTAGTTGACGGA+AGG | - | chr3_3:9258436-9258455 | Msa0441030:CDS | 50.0% | |
| AGGAAAGGTAACGACAACGG+CGG | - | chr3_3:9258456-9258475 | Msa0441030:CDS | 50.0% | |
| !!! | CAACGGCGGTTTTGTTTCGA+CGG | - | chr3_3:9258470-9258489 | Msa0441030:CDS | 50.0% |
| ! | AATCACGCTGTTAGCTTCGG+TGG | - | chr3_3:9258516-9258535 | Msa0441030:CDS | 50.0% |
| TGTTGCGAGTTTATGGCTGC+CGG | - | chr3_3:9258555-9258574 | Msa0441030:CDS | 50.0% | |
| GTTTATGGCTGCCGGTGAAA+AGG | - | chr3_3:9258563-9258582 | Msa0441030:CDS | 50.0% | |
| !!! | GCGGTTTTGTTTCGACGGTG+AGG | - | chr3_3:9258475-9258494 | Msa0441030:CDS | 55.0% |
| TTCGGTGGCCACGCTGTTGA+CGG | - | chr3_3:9258531-9258550 | Msa0441030:CDS | 60.0% | |
| CGCAACAACCGTCAACAGCG+TGG | + | chr3_3:9258542-9258561 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 9258324 | 9258776 | 9258324 | ID=Msa0441030;Name=Msa0441030 |
| chr3_3 | mRNA | 9258324 | 9258776 | 9258324 | ID=Msa0441030-mRNA-1;Parent=Msa0441030;Name=Msa0441030-mRNA-1;_AED=0.29;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|150 |
| chr3_3 | exon | 9258324 | 9258776 | 9258324 | ID=Msa0441030-mRNA-1:exon:1868;Parent=Msa0441030-mRNA-1 |
| chr3_3 | CDS | 9258324 | 9258776 | 9258324 | ID=Msa0441030-mRNA-1:cds;Parent=Msa0441030-mRNA-1 |
| Gene Sequence |
| Protein sequence |
