AlfalfaGEDB | Msa0442470

M. sativa cultivar zhongmu-4 / Msa0442470

KEGG: K14514
GO: GO:0000160 GO:0000976 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010182 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0036293 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:1990837 GO:2000112 GO:2001141
PFAM: PF04873.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0442470	AES68615.2	76.718	524	48	9	75	545	1	503	0.0	786

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0442470	sp\|Q9SLH0\|EIL1_ARATH	50.936	267	111	4	30	279	45	308	1.97e-91	294

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0442470	G7IVH7	76.718	524	48	9	75	545	1	503	0.0	786

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TFs/TRs:

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Gene ID	Type	Classification
Msa0442470	TF	EIL

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0442470	MtrunA17_Chr3g0080401	85.838	346	34	5	75	418	1	333	0.0	604
Msa0442470	MtrunA17_Chr6g0476301	48.907	366	162	7	75	424	1	357	1.74e-104	318
Msa0442470	MtrunA17_Chr6g0473191	60.504	238	92	2	75	310	1	238	5.90e-95	291
Msa0442470	MtrunA17_Chr3g0118361	48.252	286	132	4	33	305	51	333	1.59e-92	296
Msa0442470	MtrunA17_Chr6g0476291	58.403	238	97	2	75	310	1	238	1.24e-91	281
Msa0442470	MtrunA17_Chr5g0440591	48.432	287	133	4	30	302	45	330	1.68e-91	293
Msa0442470	MtrunA17_Chr2g0329151	50.382	262	108	6	30	274	34	290	6.75e-85	275
Msa0442470	MtrunA17_Chr3g0124291	46.586	249	122	3	34	271	32	280	9.14e-77	248
Msa0442470	MtrunA17_Chr8g0382561	48.062	258	118	6	27	271	17	271	6.49e-71	234
Msa0442470	MtrunA17_Chr7g0260281	35.246	122	66	4	67	177	1	120	2.14e-13	67.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0442470	AT2G27050.1	50.936	267	111	4	30	279	45	308	2.00e-92	294
Msa0442470	AT3G20770.1	49.129	287	131	4	30	302	45	330	1.92e-91	293
Msa0442470	AT1G73730.2	45.928	307	129	7	29	305	16	315	5.24e-84	271
Msa0442470	AT1G73730.1	45.928	307	129	7	29	305	28	327	5.72e-84	272
Msa0442470	AT5G10120.1	42.586	263	130	4	27	271	16	275	2.96e-70	233
Msa0442470	AT5G21120.3	39.446	289	150	4	39	302	53	341	1.05e-66	225
Msa0442470	AT5G21120.1	39.446	289	150	4	39	302	53	341	1.05e-66	225
Msa0442470	AT5G21120.2	39.446	289	150	4	39	302	60	348	1.26e-66	225
Msa0442470	AT5G65100.1	40.647	278	133	5	26	273	32	307	7.79e-66	224

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Find 109 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCACATGATTTGAAGA+AGG	0.473630	3_3:-12008297	Msa0442470:CDS
AAACATAGATATGACTATTG+AGG	0.565786	3_3:-12010348	Msa0442470:CDS
AAAGCGGCATTCCTCCACCA+TGG	0.708484	3_3:-12008391	Msa0442470:CDS
AACAACCAATTCCAAATGGT+TGG	0.519990	3_3:-12007798	Msa0442470:CDS
AACAATGCAAAACAAGATAA+AGG	0.381513	3_3:-12010395	Msa0442470:CDS
AACATGATTCCTACTCCTTC+TGG	0.428508	3_3:-12007313	Msa0442470:CDS
AACCTAATATTGTATCAGGT+AGG	0.686602	3_3:+12008471	None:intergenic
AACCTCCATGATGATTGTCT+TGG	0.533086	3_3:+12007898	None:intergenic
AAGAGAAAGGCTCACCAAAT+AGG	0.383738	3_3:-12007834	Msa0442470:CDS
AATCAGATCACTATTCATGC+AGG	0.564840	3_3:-12007699	Msa0442470:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTGACAATAAAACATCAC+TGG	-	chr3_3:12007122-12007141	Msa0442470:CDS	30.0%
AGCCATATTAATATACCTAG+TGG	+	chr3_3:12007531-12007550	None:intergenic	30.0%
CCGAAAATGACCTATATTAT+AGG	+	chr3_3:12007925-12007944	None:intergenic	30.0%
TTAAACTTGAAAATGATCCC+TGG	+	chr3_3:12008303-12008322	None:intergenic	30.0%
CAAGTCAAAGTTCTCAAAAT+TGG	+	chr3_3:12008601-12008620	None:intergenic	30.0%
AACCTAATATTGTATCAGGT+AGG	+	chr3_3:12008915-12008934	None:intergenic	30.0%
AAAACCACATGATTTGAAGA+AGG	-	chr3_3:12009086-12009105	Msa0442470:intron	30.0%
TTGGAAAATTTGTGTGTTGA+CGG	-	chr3_3:12009110-12009129	Msa0442470:intron	30.0%
TGAAAGTAGTTATGAGTTTG+TGG	-	chr3_3:12009314-12009333	Msa0442470:intron	30.0%
ATGCATGACAAATGTAGATA+GGG	+	chr3_3:12009776-12009795	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	12006955	12010450	12006955	ID=Msa0442470;Name=Msa0442470
chr3_3	mRNA	12006955	12010450	12006955	ID=Msa0442470-mRNA-1;Parent=Msa0442470;Name=Msa0442470-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|0.5\|0.33\|3\|0\|545
chr3_3	exon	12010282	12010450	12010282	ID=Msa0442470-mRNA-1:exon:2632;Parent=Msa0442470-mRNA-1
chr3_3	exon	12007700	12008782	12007700	ID=Msa0442470-mRNA-1:exon:2631;Parent=Msa0442470-mRNA-1
chr3_3	exon	12006955	12007340	12006955	ID=Msa0442470-mRNA-1:exon:2630;Parent=Msa0442470-mRNA-1
chr3_3	CDS	12010282	12010450	12010282	ID=Msa0442470-mRNA-1:cds;Parent=Msa0442470-mRNA-1
chr3_3	CDS	12007700	12008782	12007700	ID=Msa0442470-mRNA-1:cds;Parent=Msa0442470-mRNA-1
chr3_3	CDS	12006955	12007340	12006955	ID=Msa0442470-mRNA-1:cds;Parent=Msa0442470-mRNA-1

Gene Sequence

>Msa0442470

ATGCATCCCAACGTTGATAACGGTGGTGCTTCAAACAATGCAAAACAAGATAAAGGAAAGAAAAAAGAAGAAGGTGGTGAAAACATAGATATGACTATTGAGGAACTAGAGACAAAGATATGGAAAGATAAAATGCTCTTAAAGAAGATGAAGTTAAAAAGAATTCAAAATAAACAAAGTCAAATTCTCCCCGTTGAGCTGACACGTAGTGTCGACAGGTACATGTTAAAGATGGTGGAATTTTGTGATGTTCGTGGCTTCGTCTATGGAGTAGTACCTGACAATGGCAAATCAATGATTGGTTGTTCGGAAAATCTACGCGGCTGGTGGAAAGACCAAGTAAACTTTGAGGAAAACGGTCCAGCTGCAATCGCGAAATATGAAGAAGAATGTGGTTCAGTTTCAACAGTGAATCGCATGTTGCTTGATGAGCAAGTAAGGCCTTACTCCTTGTATGACCTACCTGATACAATATTAGGTTCAATTGTATCATCATTAATGCAACACTGCGATCCACCCCAAAGAAAACATCCTTTGGAAAGCGGCATTCCTCCACCATGGTGGCCAACAGGGAACGAATCATGGTGGAAGGAAATGGGATTTAGTAAGGATCTTGTCCCACCACCATACAAAAAACCACATGATTTGAAGAAGGTTTGGAAAATTTGTGTGTTGACGGCTATTATCAAACACATATCTCCTAATTTTCAGAAGATCAGGAGTATTGTTAGACATTCCAGGAGCTTGCAAAATAAATTCAATGCTAAGGACACAGCTATTTGGTTAGCTGTTATTAGTTATGAAGAAAGATTGGCAAGAGAAATGTATCCTGAATCATTCTTAAATTCTTCTTGTGTTGGTGAAAGTAGTTATGAGTTTGTGGAAACAGATGATTATGATGTGGAATGTGATGAGCACAATCTAGCAAAACCTTTATCATCATGTGATGAGGGTTCTGATACCAACATGGTTGATCAACTTGAAGAAACTTCTTCTCATCTCGAGACTTCGATATGCATTGATCCACATTACCAAGACAATCATCATGGAGGTTCTTCAATGAACAATCTTGTTATTGAGAGTAGGGGTGCTAATAAGAGAAAGGCTCACCAAATAGGAGGAAGCAGCAACAACAACCAATTCCAAATGGTTGGAGTTGGAAGCTCAAAGAATCAACAACATGGTGCACCTCTTGACCAACATGAGCAAGCTGCAGTACCTTCTGTTGCAAATCAGATCACTATTCATGCAGCTGGCAACATGATTCCTACTCCTTCTGGTGATTACCAAAATTTTCAGCCTCAAATCTACACTTCAATGATGAATACTTCTTTGCCAGTTACCAACATGATTCCAACTCCTGGATTTAACCAAAACATGCAGCCTCAAATGGAGCAGAATTTCTATGATCAACAAGGGGATGCAAATAGCAATTACAACTACAAAGTGCCTAATGCTGAAGTCGTTAATGTTCCAATGCATGAAAATGTCTCAACAATGACAACCTTCGATTCATCTTTAGATGAGTTGATGGCATTCAATTCTCAATCTGATGTTGATGCTTATGCACTTATTGATTCTCCTAATGTGGCAACACAAAGTTATGATTTCTCTTGGCCAAACAATATTGGGAATTAA

Protein sequence

>Msa0442470

MHPNVDNGGASNNAKQDKGKKKEEGGENIDMTIEELETKIWKDKMLLKKMKLKRIQNKQSQILPVELTRSVDRYMLKMVEFCDVRGFVYGVVPDNGKSMIGCSENLRGWWKDQVNFEENGPAAIAKYEEECGSVSTVNRMLLDEQVRPYSLYDLPDTILGSIVSSLMQHCDPPQRKHPLESGIPPPWWPTGNESWWKEMGFSKDLVPPPYKKPHDLKKVWKICVLTAIIKHISPNFQKIRSIVRHSRSLQNKFNAKDTAIWLAVISYEERLAREMYPESFLNSSCVGESSYEFVETDDYDVECDEHNLAKPLSSCDEGSDTNMVDQLEETSSHLETSICIDPHYQDNHHGGSSMNNLVIESRGANKRKAHQIGGSSNNNQFQMVGVGSSKNQQHGAPLDQHEQAAVPSVANQITIHAAGNMIPTPSGDYQNFQPQIYTSMMNTSLPVTNMIPTPGFNQNMQPQMEQNFYDQQGDANSNYNYKVPNAEVVNVPMHENVSTMTTFDSSLDELMAFNSQSDVDAYALIDSPNVATQSYDFSWPNNIGN*