AlfalfaGEDB | Msa0442730

M. sativa cultivar zhongmu-4 / Msa0442730

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0442730	RHN65530.1	91.384	383	22	3	1	379	21	396	0.0	718

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0442730	sp\|Q9FFW5\|PERK8_ARATH	45.638	298	149	5	39	323	324	621	7.44e-75	248

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0442730	A0A396IK04	91.384	383	22	3	1	379	21	396	0.0	718

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0442730	MtrunA17_Chr3g0080881	91.384	383	22	3	1	379	21	396	0.0	718
Msa0442730	MtrunA17_Chr3g0080901	62.931	348	125	3	24	368	99	445	7.83e-154	443
Msa0442730	MtrunA17_Chr8g0354651	65.038	266	90	3	2	267	63	325	2.70e-117	345
Msa0442730	MtrunA17_Chr3g0093601	48.220	309	156	4	7	312	59	366	3.68e-95	288
Msa0442730	MtrunA17_Chr3g0093611	47.678	323	155	5	3	312	12	333	1.75e-90	276
Msa0442730	MtrunA17_Chr8g0341881	48.837	301	146	4	24	316	375	675	2.20e-89	285
Msa0442730	MtrunA17_Chr2g0330471	48.173	301	148	4	24	316	377	677	4.38e-89	283
Msa0442730	MtrunA17_Chr3g0093591	48.387	310	154	4	15	319	95	403	1.10e-88	274
Msa0442730	MtrunA17_Chr8g0389251	48.173	301	148	4	24	316	374	674	2.66e-88	280
Msa0442730	MtrunA17_Chr7g0221191	44.295	298	155	5	35	321	376	673	5.34e-74	244

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0442730	AT5G56790.1	49.502	301	144	5	24	316	360	660	1.00e-89	284
Msa0442730	AT1G55200.3	48.173	301	148	4	24	316	349	649	2.69e-87	278
Msa0442730	AT1G55200.4	48.173	301	148	4	24	316	349	649	2.69e-87	278
Msa0442730	AT1G55200.1	48.173	301	148	4	24	316	349	649	2.69e-87	278
Msa0442730	AT1G55200.2	48.173	301	148	4	24	316	349	649	2.69e-87	278
Msa0442730	AT3G13690.2	47.841	301	149	4	24	316	381	681	5.90e-86	276
Msa0442730	AT3G13690.1	47.841	301	149	4	24	316	381	681	5.90e-86	276
Msa0442730	AT3G13690.3	47.841	301	149	4	24	316	381	681	5.90e-86	276
Msa0442730	AT5G38560.1	45.638	298	149	5	39	323	324	621	7.56e-76	248
Msa0442730	AT1G23540.1	42.405	316	162	6	12	316	338	644	4.08e-74	244

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Find 66 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGATGTATATTCTTT+TGG	0.214358	3_3:+12817114	Msa0442730:CDS
AACCTTTATGCTGGCAAGAT+AGG	0.328223	3_3:+12816483	Msa0442730:CDS
AAGAACTTTCTATCAGAAGG+TGG	0.602551	3_3:+12815945	Msa0442730:CDS
AATAGAATGTCCTGGAAATC+TGG	0.355814	3_3:+12817047	Msa0442730:CDS
AATGGTGAAAGTGGAAATAA+TGG	0.380348	3_3:+12819202	Msa0442730:CDS
ACAAGAGACCAAAACTTAAT+TGG	0.236570	3_3:+12815865	Msa0442730:CDS
ACCCTATCTTGCCAGCATAA+AGG	0.403128	3_3:-12816485	None:intergenic
ACCTTCCTCTATATGCATTA+AGG	0.281208	3_3:-12819086	None:intergenic
ACCTTTATGCTGGCAAGATA+GGG	0.495619	3_3:+12816484	Msa0442730:CDS
ACTACTGACAAAAGACTAGG+AGG	0.629115	3_3:+12817171	Msa0442730:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAGAGACCAAAACTTAAT+TGG	+	chr3_3:12815865-12815884	Msa0442730:CDS	30.0%
GAAAATATTGTCATGTTGCT+TGG	+	chr3_3:12816095-12816114	Msa0442730:CDS	30.0%
ATTCATAAACAAGAAGCCTA+TGG	-	chr3_3:12816139-12816158	None:intergenic	30.0%
GTTTATGAATATGTCTGCAA+TGG	+	chr3_3:12816149-12816168	Msa0442730:CDS	30.0%
GTATTCTGCATCATCATAAT+AGG	-	chr3_3:12816456-12816475	None:intergenic	30.0%
TGTGTAAGAAGAATGTTGTT+TGG	-	chr3_3:12816584-12816603	None:intergenic	30.0%
TACACATGATTATGAAGCTA+TGG	+	chr3_3:12816598-12816617	Msa0442730:CDS	30.0%
TGTACTTTCAGATAGTATTG+AGG	-	chr3_3:12817223-12817242	None:intergenic	30.0%
GTCAACTTTCTCTTGTAAAT+AGG	+	chr3_3:12817550-12817569	Msa0442730:intron	30.0%
ATGAAAAATAATACTCCCTC+CGG	-	chr3_3:12817577-12817596	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	12815777	12819282	12815777	ID=Msa0442730;Name=Msa0442730
chr3_3	mRNA	12815777	12819282	12815777	ID=Msa0442730-mRNA-1;Parent=Msa0442730;Name=Msa0442730-mRNA-1;_AED=0.04;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|5\|0\|379
chr3_3	exon	12815777	12816194	12815777	ID=Msa0442730-mRNA-1:exon:2749;Parent=Msa0442730-mRNA-1
chr3_3	exon	12816471	12816619	12816471	ID=Msa0442730-mRNA-1:exon:2750;Parent=Msa0442730-mRNA-1
chr3_3	exon	12816997	12817212	12816997	ID=Msa0442730-mRNA-1:exon:2751;Parent=Msa0442730-mRNA-1
chr3_3	exon	12817968	12818117	12817968	ID=Msa0442730-mRNA-1:exon:2752;Parent=Msa0442730-mRNA-1
chr3_3	exon	12819076	12819282	12819076	ID=Msa0442730-mRNA-1:exon:2753;Parent=Msa0442730-mRNA-1
chr3_3	CDS	12815777	12816194	12815777	ID=Msa0442730-mRNA-1:cds;Parent=Msa0442730-mRNA-1
chr3_3	CDS	12816471	12816619	12816471	ID=Msa0442730-mRNA-1:cds;Parent=Msa0442730-mRNA-1
chr3_3	CDS	12816997	12817212	12816997	ID=Msa0442730-mRNA-1:cds;Parent=Msa0442730-mRNA-1
chr3_3	CDS	12817968	12818117	12817968	ID=Msa0442730-mRNA-1:cds;Parent=Msa0442730-mRNA-1
chr3_3	CDS	12819076	12819282	12819076	ID=Msa0442730-mRNA-1:cds;Parent=Msa0442730-mRNA-1

Gene Sequence

>Msa0442730

ATGTCTCTCACTCATCATAACAACCATGAAGAACATCATCAAATAGTTGAACAAACATCAGAGAATTCTGTTTGTTCAATGTGCTACAACAAGAGACCAAAACTTAATTGGACAAGAAAAGAGTTCACATATGCTGAATTATATGCAGCAACACAAGGTTTTTCCATAAAGAACTTTCTATCAGAAGGTGGATTTGGTTCAGTCTACAAAGGGGAGATTCATGGACAGAAGATTGCTGTTAAGCAATGTTTACATGCAAGTCACAAACAAGAGAAGGAGTTTATGTCTGAAGTTGATGCATTAAGCAAAGCAAGACATGAAAATATTGTCATGTTGCTTGGATTATGTTCTGAAGGAAACCATAGGCTTCTTGTTTATGAATATGTCTGCAATGGTTCACTTGATCAACATTTATCACAATACTCTCGTAAACCTTTATGCTGGCAAGATAGGGTGAAAGTAGCTAATGGAGCTGCCAAAGGATTGCTATACCTTCATCAGAACAACATCATACATAGAGATATAAAACCAAACAACATTCTTCTTACACATGATTATGAAGCTATGCTGGGAGATTTTGGTTTGGCTAAAATTGCAAGTGAAGAATTCTCATGCTCAATAGAATGTCCTGGAAATCTGGCATATTGGGCACCAGAATATGCAGCATATGGTAAAGTATCAAATAAAACAGATGTATATTCTTTTGGAGTGGTTCTAGTTGAGCTAATAACTGGAATGAGGACTACTGACAAAAGACTAGGAGGAAAGGGTCTAGTAGGATGGGCTAGACCACTACTAAGAGAAGGGAATTGCCAAAAACTTGTTGATGGAAGGATAATGGATTCACATGATTGTCATCAAATCTTTTGGATGAGTCGTCTAGCTGGAAATTGTCTAAACAAAGATCCTCATAAACGACTTGATATGAATACAGTGGTCAAAGCCTTAATGCATATAGAGGAAGGTTGCAGTAGCTGCATTGTTGAAAAAGACAGTAGTACCCTTCCCATGTCAGACTATTCTAAATCAAAAGATGATATGCAGGACTCTTCAAAATCAAATGGTGAAAGTGGAAATAATGGTAGTAGTAGTTCCAGGTCCAACACAGCATATTGCTACTATAAATCTAGCAAAAATTAA

Protein sequence

>Msa0442730

MSLTHHNNHEEHHQIVEQTSENSVCSMCYNKRPKLNWTRKEFTYAELYAATQGFSIKNFLSEGGFGSVYKGEIHGQKIAVKQCLHASHKQEKEFMSEVDALSKARHENIVMLLGLCSEGNHRLLVYEYVCNGSLDQHLSQYSRKPLCWQDRVKVANGAAKGLLYLHQNNIIHRDIKPNNILLTHDYEAMLGDFGLAKIASEEFSCSIECPGNLAYWAPEYAAYGKVSNKTDVYSFGVVLVELITGMRTTDKRLGGKGLVGWARPLLREGNCQKLVDGRIMDSHDCHQIFWMSRLAGNCLNKDPHKRLDMNTVVKALMHIEEGCSSCIVEKDSSTLPMSDYSKSKDDMQDSSKSNGESGNNGSSSSRSNTAYCYYKSSKN*