AlfalfaGEDB | Msa0444430

M. sativa cultivar zhongmu-4 / Msa0444430

KEGG: -
GO: -
PFAM: PF00954.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444430	XP_039687783.1	78.959	442	78	3	11	449	1070	1499	0.0	704
Msa0444430	XP_039687783.1	88.462	442	48	1	11	449	231	672	0.0	808

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444430	sp\|O81832\|Y4729_ARATH	46.953	443	187	6	14	449	229	630	1.30e-136	414

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444430	G7IYX8	88.462	442	48	1	11	449	231	672	0.0	808

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0444430	MtrunA17_Chr3g0084441	88.462	442	48	1	11	449	231	672	808
Msa0444430	MtrunA17_Chr3g0084451	85.714	448	55	2	11	449	225	672	784
Msa0444430	MtrunA17_Chr3g0084411	81.674	442	77	2	11	449	225	665	752
Msa0444430	MtrunA17_Chr3g0084421	82.579	442	74	1	11	449	187	628	734
Msa0444430	MtrunA17_Chr3g0084691	76.282	468	77	4	11	449	226	688	711
Msa0444430	MtrunA17_Chr3g0084491	79.233	443	79	5	11	449	239	672	706
Msa0444430	MtrunA17_Chr3g0084431	78.959	442	78	3	11	449	231	660	703
Msa0444430	MtrunA17_Chr3g0084681	71.041	442	86	2	11	449	228	630	631
Msa0444430	MtrunA17_Chr3g0098791	76.243	362	83	1	12	370	228	589	577
Msa0444430	MtrunA17_Chr3g0084621	75.946	370	82	2	4	370	225	590	568

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444430	AT4G27290.2	47.439	449	222	6	14	449	229	676	7.22e-143	429
Msa0444430	AT4G27290.1	46.953	443	187	6	14	449	229	630	1.32e-137	414
Msa0444430	AT4G27300.1	47.912	455	217	8	4	449	222	665	2.89e-132	401
Msa0444430	AT1G65790.1	45.395	456	224	6	16	449	232	684	1.44e-129	395
Msa0444430	AT4G21380.1	43.450	458	231	6	16	449	238	691	1.42e-128	392
Msa0444430	AT1G11340.2	44.201	457	228	7	18	449	294	748	2.35e-126	384
Msa0444430	AT1G11340.3	44.201	457	228	7	18	449	294	748	4.83e-126	384
Msa0444430	AT1G11340.1	44.201	457	228	7	18	449	226	680	1.83e-125	384
Msa0444430	AT1G65800.1	45.964	446	215	8	27	449	246	688	1.01e-124	382
Msa0444430	AT1G11410.2	43.011	465	227	7	18	449	114	573	1.20e-116	358

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Find 150 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATTACTAGGGCGTAA+TGG	0.477204	3_3:-17358090	Msa0444430:intron
AAACAAAACTAGCAGGTTTG+TGG	0.465216	3_3:+17362017	None:intergenic
AAACAAGGGTTTCATTAAAT+TGG	0.147619	3_3:+17361788	None:intergenic
AAACCAAAATCTGCTATCTT+TGG	0.231728	3_3:+17357619	None:intergenic
AAAGTAATCCAAGCTTCTGT+TGG	0.546128	3_3:+17357896	None:intergenic
AAATTCGAAACAAAACTAGC+AGG	0.579455	3_3:+17362010	None:intergenic
AACATCGGCACAAGGAACCG+AGG	0.668089	3_3:-17358030	Msa0444430:CDS
AACCAAAATCTGCTATCTTT+GGG	0.378597	3_3:+17357620	None:intergenic
AACCACGATGCTGACGTATC+TGG	0.347266	3_3:+17359089	None:intergenic
AACGCCAATACTTCGGTATA+TGG	0.306717	3_3:-17361717	Msa0444430:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGATGGAATTGAAAAGGAAA+GGG	+	chr3_3:17357518-17357537	None:intergenic	30.0%
TTCTAGTGTTACGTGTAATA+CGG	+	chr3_3:17357542-17357561	None:intergenic	30.0%
AAATTCGAAACAAAACTAGC+AGG	+	chr3_3:17357626-17357645	None:intergenic	30.0%
ATTGAAAAAACCTGCTTCTA+AGG	+	chr3_3:17357883-17357902	None:intergenic	30.0%
GAAGATAAGGTCAAACTTAA+AGG	-	chr3_3:17358218-17358237	Msa0444430:CDS	30.0%
GTCCAAAACATGATCTATAA+TGG	+	chr3_3:17358368-17358387	None:intergenic	30.0%
CATTAGACTTAGAGAAATGT+GGG	+	chr3_3:17358489-17358508	None:intergenic	30.0%
CAATTCATCAAGAATTGTAG+CGG	-	chr3_3:17358706-17358725	Msa0444430:intron	30.0%
AAGAACAACAACGTATATCA+AGG	-	chr3_3:17358859-17358878	Msa0444430:intron	30.0%
AAATTGCAAAAACAACAGCA+TGG	-	chr3_3:17359006-17359025	Msa0444430:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	17357511	17362144	17357511	ID=Msa0444430;Name=Msa0444430
chr3_3	mRNA	17357511	17362144	17357511	ID=Msa0444430-mRNA-1;Parent=Msa0444430;Name=Msa0444430-mRNA-1;_AED=0.16;_eAED=0.16;_QI=868\|1\|1\|1\|0.8\|0.66\|6\|47\|449
chr3_3	exon	17357511	17357796	17357511	ID=Msa0444430-mRNA-1:exon:3688;Parent=Msa0444430-mRNA-1
chr3_3	exon	17357889	17358099	17357889	ID=Msa0444430-mRNA-1:exon:3687;Parent=Msa0444430-mRNA-1
chr3_3	exon	17358186	17358340	17358186	ID=Msa0444430-mRNA-1:exon:3686;Parent=Msa0444430-mRNA-1
chr3_3	exon	17358506	17358622	17358506	ID=Msa0444430-mRNA-1:exon:3685;Parent=Msa0444430-mRNA-1
chr3_3	exon	17358898	17359919	17358898	ID=Msa0444430-mRNA-1:exon:3684;Parent=Msa0444430-mRNA-1
chr3_3	exon	17361671	17362144	17361671	ID=Msa0444430-mRNA-1:exon:3683;Parent=Msa0444430-mRNA-1
chr3_3	five_prime_UTR	17361671	17362144	17361671	ID=Msa0444430-mRNA-1:five_prime_utr;Parent=Msa0444430-mRNA-1
chr3_3	five_prime_UTR	17359526	17359919	17359526	ID=Msa0444430-mRNA-1:five_prime_utr;Parent=Msa0444430-mRNA-1
chr3_3	CDS	17358898	17359525	17358898	ID=Msa0444430-mRNA-1:cds;Parent=Msa0444430-mRNA-1
chr3_3	CDS	17358506	17358622	17358506	ID=Msa0444430-mRNA-1:cds;Parent=Msa0444430-mRNA-1
chr3_3	CDS	17358186	17358340	17358186	ID=Msa0444430-mRNA-1:cds;Parent=Msa0444430-mRNA-1
chr3_3	CDS	17357889	17358099	17357889	ID=Msa0444430-mRNA-1:cds;Parent=Msa0444430-mRNA-1
chr3_3	CDS	17357558	17357796	17357558	ID=Msa0444430-mRNA-1:cds;Parent=Msa0444430-mRNA-1
chr3_3	three_prime_UTR	17357511	17357557	17357511	ID=Msa0444430-mRNA-1:three_prime_utr;Parent=Msa0444430-mRNA-1

Gene Sequence

>Msa0444430

ATGGAATGGATTTCTTTTCACTGGTGTTTTTCTTGGCAAAGAATGCACAGAGTCTTGAATTTCTCAGTAGTGTTCACTGACAAAGAATTCTCTTATCAATATGAAACTAGGAACAGTTCAATTATTACTAGAATGGAGCTAGATCCATCTGGAAATTCACGTCGTCTTCTATGGTCAGATAGTACACAACTTTGGAAGGCTATATCTTCTAGTCCTGAAGACCAGTGTGATGCGTATGAAATGTGTGGTATCAATTCTAACTGCAATGGTGATATATGTGAATGCTTAGAAGGTTTCATTCCAAAATTTCAACTAGAGTGGGAGTCATCAAATCGGTCTGGTGGATGTGTAAGAAAAACACGTTTAAATTGTCTCCATGGAGATGGATTTTTACCGTACACAAACATGACGTTGCCAGATACGTCAGCATCGTGGTTCGATAAGAGTTTGAGCCTTGAGGAATGTAAGACAAAGTGTTTGAAAAACTGTAATTGCACTGCATATGCAAATTCAGATATCAGCTATGGTGGGAGTGGCTGCTTACTCTGGTTTGACGACATTGTGGACATGAGAAAACATCAAGACCAAGGACAATTCATTTACATACGGATGGCATCTTCAGAACTTGATCATGAAGAGAATAAGCGGAAATTGAAGCTTGCCGGGACTCTTGCAGGAGTTATTGCATTTATTATAGTAATAACCGTTCTCGTTTTGATCACATCAGCATATAGAAAGAAGCTTGGGTATATAAAAAAGTTATTTCTCTGGAAGCACAAGAAGGAGAAGGAATATGGTGATTTGGCAACAATATTTGATTTTTCAACCATCACTAATGCAACAAATAATTTCTCTATCAGAAACAAGTTGGGAGAAGGTGGTTTTGGACCAGTGTACAAGGGCGTAATGGTTGACGGCCAAGAGATTGCTGTTAAGAGGCTTTCTAAAACATCGGCACAAGGAACCGAGGAGTTCAAAAATGAAGTAAACTTGATGGCAACGCTTCAACACCGTAATCTTGTAAAACTTCTTGGTTGTTCTATTCGACAAGATGAAAAGTTGTTGATCTATGAATTCATGGCCAACAGAAGCTTGGATTACTTTATTTTTGATACTATGCGAAGCAAATTGCTAAATTGGATTAAGCGCCTGGAAATTATTGATGGAATTGCTCGAGGGCTGTTGTATCTTCATCAAGATTCTACACTAAGAATCATACATAGAGATGTGAAAACAAGCAATATTCTTCTTGATATTGATATGATCCCAAAGATAGCAGATTTTGGTTTAGCCAGATCATTTAAGGGAGACGAAGCAGAGGCTAATACAAATAGATTGATCGGATCATAG

Protein sequence

>Msa0444430

MEWISFHWCFSWQRMHRVLNFSVVFTDKEFSYQYETRNSSIITRMELDPSGNSRRLLWSDSTQLWKAISSSPEDQCDAYEMCGINSNCNGDICECLEGFIPKFQLEWESSNRSGGCVRKTRLNCLHGDGFLPYTNMTLPDTSASWFDKSLSLEECKTKCLKNCNCTAYANSDISYGGSGCLLWFDDIVDMRKHQDQGQFIYIRMASSELDHEENKRKLKLAGTLAGVIAFIIVITVLVLITSAYRKKLGYIKKLFLWKHKKEKEYGDLATIFDFSTITNATNNFSIRNKLGEGGFGPVYKGVMVDGQEIAVKRLSKTSAQGTEEFKNEVNLMATLQHRNLVKLLGCSIRQDEKLLIYEFMANRSLDYFIFDTMRSKLLNWIKRLEIIDGIARGLLYLHQDSTLRIIHRDVKTSNILLDIDMIPKIADFGLARSFKGDEAEANTNRLIGS*