AlfalfaGEDB | Msa0445900

M. sativa cultivar zhongmu-4 / Msa0445900

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0445900	GAU12690.1	54.717	424	123	12	9	371	116	531	2.23e-127	387

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0445900	sp\|A2WW87\|GAM1_ORYSI	51.364	220	94	3	24	240	23	232	7.26e-63	213

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0445900	A0A396JQM3	56.091	394	128	10	3	371	40	413	8.98e-120	362

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TFs/TRs:

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Gene ID	Type	Classification
Msa0445900	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0445900	MtrunA17_Chr1g0154031	56.091	394	128	10	3	371	40	413	1.73e-123	362
Msa0445900	MtrunA17_Chr8g0354661	41.009	317	143	7	41	318	37	348	1.42e-61	206
Msa0445900	MtrunA17_Chr3g0080861	39.222	334	145	9	41	329	37	357	4.21e-60	202
Msa0445900	MtrunA17_Chr3g0099131	70.940	117	34	0	39	155	39	155	6.14e-55	189
Msa0445900	MtrunA17_Chr2g0322521	67.227	119	39	0	41	159	29	147	9.75e-49	169
Msa0445900	MtrunA17_Chr1g0192411	46.114	193	93	5	41	229	12	197	5.79e-47	164
Msa0445900	MtrunA17_Chr4g0039971	63.551	107	39	0	38	144	10	116	1.98e-44	156
Msa0445900	MtrunA17_Chr5g0395281	62.617	107	40	0	38	144	9	115	2.14e-43	152
Msa0445900	MtrunA17_Chr5g0440781	44.253	174	80	4	42	214	13	170	3.72e-43	148
Msa0445900	MtrunA17_Chr7g0274581	53.968	126	57	1	41	166	12	136	4.03e-43	149

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0445900	AT3G11440.2	73.554	121	32	0	35	155	35	155	3.17e-59	199
Msa0445900	AT5G06100.1	54.011	187	83	2	24	208	15	200	2.90e-58	196
Msa0445900	AT3G11440.3	73.554	121	32	0	35	155	35	155	3.15e-58	198
Msa0445900	AT3G11440.4	73.554	121	32	0	35	155	35	155	3.15e-58	198
Msa0445900	AT3G11440.1	73.554	121	32	0	35	155	35	155	3.15e-58	198
Msa0445900	AT5G06100.5	54.011	187	83	2	24	208	15	200	1.31e-57	196
Msa0445900	AT5G06100.2	54.011	187	83	2	24	208	15	200	1.31e-57	196
Msa0445900	AT5G06100.3	54.011	187	83	2	24	208	15	200	1.31e-57	196
Msa0445900	AT5G06100.4	54.011	187	83	2	24	208	15	200	1.31e-57	196
Msa0445900	AT2G26960.1	53.143	175	79	2	28	199	7	181	1.87e-57	193

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Find 92 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCACGGAGAGGGAAAT+TGG	0.378253	3_3:-20808843	Msa0445900:CDS
AAAGAAAGGTCAATGGACGT+CGG	0.691272	3_3:-20808896	Msa0445900:CDS
AAATTTGAGTATACCTGACG+TGG	0.639048	3_3:-20807944	Msa0445900:CDS
AACATATGAACATGAACATG+TGG	0.606750	3_3:-20807432	Msa0445900:CDS
AACATGGTGGTATTATATCT+TGG	0.446158	3_3:+20807899	None:intergenic
AACTACGGCGCCCAATGAAG+CGG	0.599129	3_3:-20807527	Msa0445900:CDS
AAGCAGCAGACTGATCGTCT+AGG	0.617912	3_3:+20807482	None:intergenic
AAGCGGACGTTAGTACACAA+TGG	0.647493	3_3:-20807510	Msa0445900:CDS
AATGACATGCTAAACCTAAG+GGG	0.680724	3_3:+20807381	None:intergenic
AATGGAGGAGACCACATAAT+AGG	0.371722	3_3:+20807760	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAGCTCATGTTAAAAAGCA+CGG	-	chr3_3:20807558-20807577	Msa0445900:CDS	30.0%
AACATGGTGGTATTATATCT+TGG	+	chr3_3:20808518-20808537	None:intergenic	30.0%
GCGTAAAATATCCTATTATG+TGG	-	chr3_3:20808643-20808662	Msa0445900:intron	30.0%
TCAAGGATATAATAACCTTC+TGG	-	chr3_3:20808686-20808705	Msa0445900:CDS	30.0%
AGAAGAAGAAATATTTGACG+TGG	+	chr3_3:20808724-20808743	None:intergenic	30.0%
ACTTGCAATGAATCAAGTAA+AGG	+	chr3_3:20808858-20808877	None:intergenic	30.0%
AACATATGAACATGAACATG+TGG	-	chr3_3:20808982-20809001	Msa0445900:CDS	30.0%
ATAGCTCATGTTAAAAAGCA+CGG	-	chr3_3:20807558-20807577	Msa0445900:CDS	30.0%
AACATGGTGGTATTATATCT+TGG	+	chr3_3:20808518-20808537	None:intergenic	30.0%
GCGTAAAATATCCTATTATG+TGG	-	chr3_3:20808643-20808662	Msa0445900:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	20807396	20809040	20807396	ID=Msa0445900;Name=Msa0445900
chr3_3	mRNA	20807396	20809040	20807396	ID=Msa0445900-mRNA-1;Parent=Msa0445900;Name=Msa0445900-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|0\|0\|1\|1\|1\|2\|0\|372
chr3_3	exon	20808669	20809040	20808669	ID=Msa0445900-mRNA-1:exon:4398;Parent=Msa0445900-mRNA-1
chr3_3	exon	20807396	20808142	20807396	ID=Msa0445900-mRNA-1:exon:4397;Parent=Msa0445900-mRNA-1
chr3_3	CDS	20808669	20809040	20808669	ID=Msa0445900-mRNA-1:cds;Parent=Msa0445900-mRNA-1
chr3_3	CDS	20807396	20808142	20807396	ID=Msa0445900-mRNA-1:cds;Parent=Msa0445900-mRNA-1

Gene Sequence

>Msa0445900

ATGAGTAGTAGCGCTTCAAGCGGGGATGATGATCGCAAAAGGCTAAAGCGCGGTCCGTCATCATTATCAGCTGAAGAAGGTGATCAAGGAGATAGCACTGGAAGAGGAGGCGAAGGTAGTCTAAAGAAAGGTCAATGGACGTCGGCAGAGGATGATATTTTGATAGCTCATGTTAAAAAGCACGGAGAGGGAAATTGGAGTGCAGTCCAAAAGTATACAGGACTTGCTCGTTGTGGGAAAAGCTGTCGTCTACGATGGGCAAACCATTTGAGACCAGATCTGAGAAAGGGTGCAATTACTGCTGAAGAAGAACGCCGAATCCTGGAGTTGCACTTTAAGATGGGAAATAAATGGGCTCAAATTGCTGCACTGTTGCCTGGACGTACAGATAATGAGATAAAGAACTTTTGGAACACAAGATATAAGAAACGAACACGAGCTAACTTACCAGTCTACCCTGAAGAGACAAGATCCAAACGACCATTGAATGAGAATCAAGAAAATGCCGACACATTCACAAATGAAGCCAGCCAGAGTGATGAAACAGAAAATTTGAGTATACCTGACGTGGAATTCAAAGATTACAAACTCCGCCAAGATATAATACCACCATGTTTTGATATATGGGGAAGTGGCTTGCTTGGACATCGTTCAGATTCAACATGCAATAATTTGTACGGTTGTAGCAGTAGTGCTGCTGTCCCAGCAACATTTGATCAGTGCGTAAAATATCCTATTATGTGGTCTCCTCCATTTAATCAGTATCAAGGATATAATAACCTTCTGGTTGGTATTCATGCCACGTCAAATATTTCTTCTTCTGAGCTCCCTTCACTCCAATATTTGCCGACTCAACATTATAGCTGGGGCTGCATGCCTGCGTTGATTCAGCCTCCTCCGTTTGAGCCTTATCAGTCAGTTCTAACTTCTCCACCTTTACTTGATTCATTGCAAGTGAAAACTGAAACTACGGCGCCCAATGAAGCGGACGTTAGTACACAATGGGCCGACCTAGACGATCAGTCTGCTGCTTCAATTCTGTCATCTGACTATAACACACAACATATGAACATGAACATGTGGTCAATTGATGGGCCCCACTCATTTGAAACCCCTTAG

Protein sequence

>Msa0445900

MSSSASSGDDDRKRLKRGPSSLSAEEGDQGDSTGRGGEGSLKKGQWTSAEDDILIAHVKKHGEGNWSAVQKYTGLARCGKSCRLRWANHLRPDLRKGAITAEEERRILELHFKMGNKWAQIAALLPGRTDNEIKNFWNTRYKKRTRANLPVYPEETRSKRPLNENQENADTFTNEASQSDETENLSIPDVEFKDYKLRQDIIPPCFDIWGSGLLGHRSDSTCNNLYGCSSSAAVPATFDQCVKYPIMWSPPFNQYQGYNNLLVGIHATSNISSSELPSLQYLPTQHYSWGCMPALIQPPPFEPYQSVLTSPPLLDSLQVKTETTAPNEADVSTQWADLDDQSAASILSSDYNTQHMNMNMWSIDGPHSFETP*