AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0450910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0450910 MtrunA17_Chr3g0082121 58.140 86 32 1 1 82 47 132 1.45e-24 95.9
Msa0450910 MtrunA17_Chr5g0401121 60.870 69 27 0 1 69 60 128 3.31e-20 79.7
Msa0450910 MtrunA17_Chr6g0458391 45.588 68 37 0 1 68 229 296 3.20e-16 72.4
Msa0450910 MtrunA17_Chr1g0149131 86.207 29 4 0 71 99 61 89 3.83e-12 57.8
Msa0450910 MtrunA17_Chr1g0203721 38.806 67 41 0 3 69 69 135 5.54e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGCTCTAGGCTCCCCTTT+TGG 0.322934 3_3:+37401660 None:intergenic
GCAAATAAAAGCAGCGTAGA+TGG 0.362217 3_3:-37401750 Msa0450910:CDS
ACTTGTTGAGCATAATATAT+TGG 0.389708 3_3:-37400796 Msa0450910:CDS
AATGAGTACAGTAAAAGAAT+AGG 0.450823 3_3:-37401795 Msa0450910:CDS
GATAAAAGACGTCAACCAAA+AGG 0.461894 3_3:-37401675 Msa0450910:CDS
TGCATAAATCTACATGAAGA+TGG 0.470430 3_3:-37400849 Msa0450910:CDS
CTCGTTCTAATTTCTGCTCT+AGG 0.476581 3_3:+37401648 None:intergenic
GCATAAATCTACATGAAGAT+GGG 0.505040 3_3:-37400848 Msa0450910:CDS
ATAAAAGACGTCAACCAAAA+GGG 0.517756 3_3:-37401674 Msa0450910:CDS
GTTGAGCATAATATATTGGA+AGG 0.525933 3_3:-37400792 Msa0450910:CDS
CATAAATCTACATGAAGATG+GGG 0.584470 3_3:-37400847 Msa0450910:CDS
ATAATATATTGGAAGGGTCG+TGG 0.588775 3_3:-37400785 None:intergenic
AGATTGACATGCTTCAAAGA+GGG 0.606059 3_3:-37401711 Msa0450910:CDS
TTGAGCATAATATATTGGAA+GGG 0.622690 3_3:-37400791 Msa0450910:CDS
TGCTTCAAAGAGGGTGTACG+AGG 0.626130 3_3:-37401702 Msa0450910:CDS
TAAAAGACGTCAACCAAAAG+GGG 0.631119 3_3:-37401673 Msa0450910:CDS
AAGATTGACATGCTTCAAAG+AGG 0.631174 3_3:-37401712 Msa0450910:CDS
AATGAAGATTGATTCTCAAG+CGG 0.660742 3_3:-37401835 Msa0450910:CDS
ATAAATCTACATGAAGATGG+GGG 0.732388 3_3:-37400846 Msa0450910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCAAATAACAAATATATTT+TGG - chr3_3:37401382-37401401 Msa0450910:intron 15.0%
!!! GCAAATAACAAATATATTTT+GGG - chr3_3:37401383-37401402 Msa0450910:intron 15.0%
!!! AGCAAATAACAAATATATTT+TGG - chr3_3:37401382-37401401 Msa0450910:intron 15.0%
!!! GCAAATAACAAATATATTTT+GGG - chr3_3:37401383-37401402 Msa0450910:intron 15.0%
!!! ATATATTCTTGCAAATCTTT+TGG + chr3_3:37401181-37401200 None:intergenic 20.0%
!!! AATAACAAATATATTTTGGG+TGG - chr3_3:37401386-37401405 Msa0450910:intron 20.0%
!! CACAAATGATAAATGATTAT+GGG - chr3_3:37401412-37401431 Msa0450910:intron 20.0%
!!! ATAAATGATTATGGGTATTT+TGG - chr3_3:37401420-37401439 Msa0450910:intron 20.0%
!!! ATATATTCTTGCAAATCTTT+TGG + chr3_3:37401181-37401200 None:intergenic 20.0%
!!! AATAACAAATATATTTTGGG+TGG - chr3_3:37401386-37401405 Msa0450910:intron 20.0%
!! CACAAATGATAAATGATTAT+GGG - chr3_3:37401412-37401431 Msa0450910:intron 20.0%
!!! ATAAATGATTATGGGTATTT+TGG - chr3_3:37401420-37401439 Msa0450910:intron 20.0%
! AATGAGTACAGTAAAAGAAT+AGG - chr3_3:37400827-37400846 Msa0450910:CDS 25.0%
!! GTTCTTTTATGACTTTCAAA+TGG - chr3_3:37401350-37401369 Msa0450910:intron 25.0%
! GCACAAATGATAAATGATTA+TGG - chr3_3:37401411-37401430 Msa0450910:intron 25.0%
!! GTTATTTGAAACATTTCTCA+AGG - chr3_3:37401587-37401606 Msa0450910:intron 25.0%
! ATATAAGACATAACGCTTTA+CGG - chr3_3:37401696-37401715 Msa0450910:CDS 25.0%
!! ATTGTGAACTCTTTTGATAT+AGG + chr3_3:37401733-37401752 None:intergenic 25.0%
!! ACTTGTTGAGCATAATATAT+TGG - chr3_3:37401826-37401845 Msa0450910:CDS 25.0%
! TTGAGCATAATATATTGGAA+GGG - chr3_3:37401831-37401850 Msa0450910:CDS 25.0%
! AATGAGTACAGTAAAAGAAT+AGG - chr3_3:37400827-37400846 Msa0450910:CDS 25.0%
!! GTTCTTTTATGACTTTCAAA+TGG - chr3_3:37401350-37401369 Msa0450910:intron 25.0%
! GCACAAATGATAAATGATTA+TGG - chr3_3:37401411-37401430 Msa0450910:intron 25.0%
!! GTTATTTGAAACATTTCTCA+AGG - chr3_3:37401587-37401606 Msa0450910:intron 25.0%
! ATATAAGACATAACGCTTTA+CGG - chr3_3:37401696-37401715 Msa0450910:CDS 25.0%
!! ATTGTGAACTCTTTTGATAT+AGG + chr3_3:37401733-37401752 None:intergenic 25.0%
!! ACTTGTTGAGCATAATATAT+TGG - chr3_3:37401826-37401845 Msa0450910:CDS 25.0%
! TTGAGCATAATATATTGGAA+GGG - chr3_3:37401831-37401850 Msa0450910:CDS 25.0%
ATAAAAGACGTCAACCAAAA+GGG - chr3_3:37400948-37400967 Msa0450910:intron 30.0%
AGAAATTAGAACGAGATGTT+CGG - chr3_3:37400982-37401001 Msa0450910:intron 30.0%
ATATATATATCCAAGCAAGC+TGG - chr3_3:37401195-37401214 Msa0450910:intron 30.0%
TGAAAGTCATAAAAGAACGA+GGG + chr3_3:37401348-37401367 None:intergenic 30.0%
TTGAAAGTCATAAAAGAACG+AGG + chr3_3:37401349-37401368 None:intergenic 30.0%
ACCACATACAAGACAAATAA+GGG - chr3_3:37401462-37401481 Msa0450910:intron 30.0%
ACAAGACAAATAAGGGTTAT+CGG - chr3_3:37401469-37401488 Msa0450910:intron 30.0%
!!! ACATGCAAACAGTCATTTTT+GGG - chr3_3:37401523-37401542 Msa0450910:intron 30.0%
TCAAATAACCACTTGAAAGA+CGG + chr3_3:37401576-37401595 None:intergenic 30.0%
AAAGCCGAAAACAATTATGA+CGG - chr3_3:37401623-37401642 Msa0450910:intron 30.0%
! TGATCCGTCATAATTGTTTT+CGG + chr3_3:37401630-37401649 None:intergenic 30.0%
AAAGAGTTCACAATTCTGTT+TGG - chr3_3:37401739-37401758 Msa0450910:CDS 30.0%
TGCATAAATCTACATGAAGA+TGG - chr3_3:37401773-37401792 Msa0450910:CDS 30.0%
GCATAAATCTACATGAAGAT+GGG - chr3_3:37401774-37401793 Msa0450910:CDS 30.0%
CATAAATCTACATGAAGATG+GGG - chr3_3:37401775-37401794 Msa0450910:CDS 30.0%
ATAAATCTACATGAAGATGG+GGG - chr3_3:37401776-37401795 Msa0450910:CDS 30.0%
GTTGAGCATAATATATTGGA+AGG - chr3_3:37401830-37401849 Msa0450910:CDS 30.0%
ATAAAAGACGTCAACCAAAA+GGG - chr3_3:37400948-37400967 Msa0450910:intron 30.0%
AGAAATTAGAACGAGATGTT+CGG - chr3_3:37400982-37401001 Msa0450910:intron 30.0%
ATATATATATCCAAGCAAGC+TGG - chr3_3:37401195-37401214 Msa0450910:intron 30.0%
TGAAAGTCATAAAAGAACGA+GGG + chr3_3:37401348-37401367 None:intergenic 30.0%
TTGAAAGTCATAAAAGAACG+AGG + chr3_3:37401349-37401368 None:intergenic 30.0%
ACCACATACAAGACAAATAA+GGG - chr3_3:37401462-37401481 Msa0450910:intron 30.0%
ACAAGACAAATAAGGGTTAT+CGG - chr3_3:37401469-37401488 Msa0450910:intron 30.0%
!!! ACATGCAAACAGTCATTTTT+GGG - chr3_3:37401523-37401542 Msa0450910:intron 30.0%
TCAAATAACCACTTGAAAGA+CGG + chr3_3:37401576-37401595 None:intergenic 30.0%
AAAGCCGAAAACAATTATGA+CGG - chr3_3:37401623-37401642 Msa0450910:intron 30.0%
! TGATCCGTCATAATTGTTTT+CGG + chr3_3:37401630-37401649 None:intergenic 30.0%
AAAGAGTTCACAATTCTGTT+TGG - chr3_3:37401739-37401758 Msa0450910:CDS 30.0%
TGCATAAATCTACATGAAGA+TGG - chr3_3:37401773-37401792 Msa0450910:CDS 30.0%
GCATAAATCTACATGAAGAT+GGG - chr3_3:37401774-37401793 Msa0450910:CDS 30.0%
CATAAATCTACATGAAGATG+GGG - chr3_3:37401775-37401794 Msa0450910:CDS 30.0%
ATAAATCTACATGAAGATGG+GGG - chr3_3:37401776-37401795 Msa0450910:CDS 30.0%
GTTGAGCATAATATATTGGA+AGG - chr3_3:37401830-37401849 Msa0450910:CDS 30.0%
AAGATTGACATGCTTCAAAG+AGG - chr3_3:37400910-37400929 Msa0450910:intron 35.0%
AGATTGACATGCTTCAAAGA+GGG - chr3_3:37400911-37400930 Msa0450910:intron 35.0%
GATAAAAGACGTCAACCAAA+AGG - chr3_3:37400947-37400966 Msa0450910:intron 35.0%
TAAAAGACGTCAACCAAAAG+GGG - chr3_3:37400949-37400968 Msa0450910:intron 35.0%
! GTATGGTTATTTCACTCGAT+CGG - chr3_3:37401008-37401027 Msa0450910:intron 35.0%
ACAAGCATCACAAATTGTCT+TGG - chr3_3:37401135-37401154 Msa0450910:intron 35.0%
CAAGCATCACAAATTGTCTT+GGG - chr3_3:37401136-37401155 Msa0450910:intron 35.0%
TGGTTTCAAGGTAATTCGTA+AGG + chr3_3:37401254-37401273 None:intergenic 35.0%
CGAATTACCTTGAAACCAAA+AGG - chr3_3:37401256-37401275 Msa0450910:intron 35.0%
TCACTAAAGTATGGTGAAGT+TGG - chr3_3:37401285-37401304 Msa0450910:intron 35.0%
CACCACATACAAGACAAATA+AGG - chr3_3:37401461-37401480 Msa0450910:intron 35.0%
! ACCCTTATTTGTCTTGTATG+TGG + chr3_3:37401466-37401485 None:intergenic 35.0%
!! CACATGCAAACAGTCATTTT+TGG - chr3_3:37401522-37401541 Msa0450910:intron 35.0%
!!! CATGCAAACAGTCATTTTTG+GGG - chr3_3:37401524-37401543 Msa0450910:intron 35.0%
CAAATAACCACTTGAAAGAC+GGG + chr3_3:37401575-37401594 None:intergenic 35.0%
TGAAACATTTCTCAAGGCAA+TGG - chr3_3:37401593-37401612 Msa0450910:intron 35.0%
GAAACATTTCTCAAGGCAAT+GGG - chr3_3:37401594-37401613 Msa0450910:intron 35.0%
GATGGCTAAGAAACATTTCA+TGG - chr3_3:37401659-37401678 Msa0450910:CDS 35.0%
! GTGAACTCTTTTGATATAGG+TGG + chr3_3:37401730-37401749 None:intergenic 35.0%
AAGATTGACATGCTTCAAAG+AGG - chr3_3:37400910-37400929 Msa0450910:intron 35.0%
AGATTGACATGCTTCAAAGA+GGG - chr3_3:37400911-37400930 Msa0450910:intron 35.0%
GATAAAAGACGTCAACCAAA+AGG - chr3_3:37400947-37400966 Msa0450910:intron 35.0%
TAAAAGACGTCAACCAAAAG+GGG - chr3_3:37400949-37400968 Msa0450910:intron 35.0%
! GTATGGTTATTTCACTCGAT+CGG - chr3_3:37401008-37401027 Msa0450910:intron 35.0%
ACAAGCATCACAAATTGTCT+TGG - chr3_3:37401135-37401154 Msa0450910:intron 35.0%
CAAGCATCACAAATTGTCTT+GGG - chr3_3:37401136-37401155 Msa0450910:intron 35.0%
TGGTTTCAAGGTAATTCGTA+AGG + chr3_3:37401254-37401273 None:intergenic 35.0%
CGAATTACCTTGAAACCAAA+AGG - chr3_3:37401256-37401275 Msa0450910:intron 35.0%
TCACTAAAGTATGGTGAAGT+TGG - chr3_3:37401285-37401304 Msa0450910:intron 35.0%
CACCACATACAAGACAAATA+AGG - chr3_3:37401461-37401480 Msa0450910:intron 35.0%
! ACCCTTATTTGTCTTGTATG+TGG + chr3_3:37401466-37401485 None:intergenic 35.0%
!! CACATGCAAACAGTCATTTT+TGG - chr3_3:37401522-37401541 Msa0450910:intron 35.0%
!!! CATGCAAACAGTCATTTTTG+GGG - chr3_3:37401524-37401543 Msa0450910:intron 35.0%
CAAATAACCACTTGAAAGAC+GGG + chr3_3:37401575-37401594 None:intergenic 35.0%
TGAAACATTTCTCAAGGCAA+TGG - chr3_3:37401593-37401612 Msa0450910:intron 35.0%
GAAACATTTCTCAAGGCAAT+GGG - chr3_3:37401594-37401613 Msa0450910:intron 35.0%
GATGGCTAAGAAACATTTCA+TGG - chr3_3:37401659-37401678 Msa0450910:CDS 35.0%
! GTGAACTCTTTTGATATAGG+TGG + chr3_3:37401730-37401749 None:intergenic 35.0%
GCAAATAAAAGCAGCGTAGA+TGG - chr3_3:37400872-37400891 Msa0450910:intron 40.0%
CTCGTTCTAATTTCTGCTCT+AGG + chr3_3:37400977-37400996 None:intergenic 40.0%
!! GAACATGCTCAGGTTGAATT+TGG + chr3_3:37401081-37401100 None:intergenic 40.0%
CGAATCATATGAACATGCTC+AGG + chr3_3:37401091-37401110 None:intergenic 40.0%
TATATATCCAAGCAAGCTGG+TGG - chr3_3:37401198-37401217 Msa0450910:intron 40.0%
GCTGGTGGAATAAAGACAAT+TGG - chr3_3:37401213-37401232 Msa0450910:intron 40.0%
!!! ACTGCTTCCTTTTGGTTTCA+AGG + chr3_3:37401266-37401285 None:intergenic 40.0%
! CTTTAGTGACTGCTTCCTTT+TGG + chr3_3:37401274-37401293 None:intergenic 40.0%
AGGAAGCAGTCACTAAAGTA+TGG - chr3_3:37401276-37401295 Msa0450910:intron 40.0%
AAACAATCCCGTCTTTCAAG+TGG - chr3_3:37401565-37401584 Msa0450910:intron 40.0%
! CATTTCATGGATTGTGCACT+TGG - chr3_3:37401672-37401691 Msa0450910:CDS 40.0%
! GGGGTGAATTTTTGAATGCT+TGG - chr3_3:37401795-37401814 Msa0450910:CDS 40.0%
GCAAATAAAAGCAGCGTAGA+TGG - chr3_3:37400872-37400891 Msa0450910:intron 40.0%
CTCGTTCTAATTTCTGCTCT+AGG + chr3_3:37400977-37400996 None:intergenic 40.0%
!! GAACATGCTCAGGTTGAATT+TGG + chr3_3:37401081-37401100 None:intergenic 40.0%
CGAATCATATGAACATGCTC+AGG + chr3_3:37401091-37401110 None:intergenic 40.0%
TATATATCCAAGCAAGCTGG+TGG - chr3_3:37401198-37401217 Msa0450910:intron 40.0%
GCTGGTGGAATAAAGACAAT+TGG - chr3_3:37401213-37401232 Msa0450910:intron 40.0%
!!! ACTGCTTCCTTTTGGTTTCA+AGG + chr3_3:37401266-37401285 None:intergenic 40.0%
! CTTTAGTGACTGCTTCCTTT+TGG + chr3_3:37401274-37401293 None:intergenic 40.0%
AGGAAGCAGTCACTAAAGTA+TGG - chr3_3:37401276-37401295 Msa0450910:intron 40.0%
AAACAATCCCGTCTTTCAAG+TGG - chr3_3:37401565-37401584 Msa0450910:intron 40.0%
! CATTTCATGGATTGTGCACT+TGG - chr3_3:37401672-37401691 Msa0450910:CDS 40.0%
! GGGGTGAATTTTTGAATGCT+TGG - chr3_3:37401795-37401814 Msa0450910:CDS 40.0%
!! CATGCTCAGGTTGAATTTGG+TGG + chr3_3:37401078-37401097 None:intergenic 45.0%
! CTTTATTCCACCAGCTTGCT+TGG + chr3_3:37401208-37401227 None:intergenic 45.0%
AGTCCAACAAATACTCCCAG+CGG + chr3_3:37401495-37401514 None:intergenic 45.0%
ACATTTCTCAAGGCAATGGG+TGG - chr3_3:37401597-37401616 Msa0450910:intron 45.0%
!! CATGCTCAGGTTGAATTTGG+TGG + chr3_3:37401078-37401097 None:intergenic 45.0%
! CTTTATTCCACCAGCTTGCT+TGG + chr3_3:37401208-37401227 None:intergenic 45.0%
AGTCCAACAAATACTCCCAG+CGG + chr3_3:37401495-37401514 None:intergenic 45.0%
ACATTTCTCAAGGCAATGGG+TGG - chr3_3:37401597-37401616 Msa0450910:intron 45.0%
! TGCTTCAAAGAGGGTGTACG+AGG - chr3_3:37400920-37400939 Msa0450910:intron 50.0%
AAATAAGGGTTATCGGCCGC+TGG - chr3_3:37401476-37401495 Msa0450910:intron 50.0%
AATAAGGGTTATCGGCCGCT+GGG - chr3_3:37401477-37401496 Msa0450910:intron 50.0%
! TGCTTCAAAGAGGGTGTACG+AGG - chr3_3:37400920-37400939 Msa0450910:intron 50.0%
AAATAAGGGTTATCGGCCGC+TGG - chr3_3:37401476-37401495 Msa0450910:intron 50.0%
AATAAGGGTTATCGGCCGCT+GGG - chr3_3:37401477-37401496 Msa0450910:intron 50.0%
!! TCTGCTCTAGGCTCCCCTTT+TGG + chr3_3:37400965-37400984 None:intergenic 55.0%
AACGAGATGTTCGGCGCGTA+TGG - chr3_3:37400991-37401010 Msa0450910:intron 55.0%
!! TCTGCTCTAGGCTCCCCTTT+TGG + chr3_3:37400965-37400984 None:intergenic 55.0%
AACGAGATGTTCGGCGCGTA+TGG - chr3_3:37400991-37401010 Msa0450910:intron 55.0%
!! CGGCCGCTGGGAGTATTTGT+TGG - chr3_3:37401489-37401508 Msa0450910:intron 60.0%
GACGGATCACGATGTCGCGA+TGG - chr3_3:37401641-37401660 Msa0450910:intron 60.0%
!! CGGCCGCTGGGAGTATTTGT+TGG - chr3_3:37401489-37401508 Msa0450910:intron 60.0%
GACGGATCACGATGTCGCGA+TGG - chr3_3:37401641-37401660 Msa0450910:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 37400788 37401856 37400788 ID=Msa0450910;Name=Msa0450910
chr3_3 mRNA 37400788 37401856 37400788 ID=Msa0450910-mRNA-1;Parent=Msa0450910;Name=Msa0450910-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|0|0|1|0|0|2|0|99
chr3_3 exon 37401646 37401856 37401646 ID=Msa0450910-mRNA-1:exon:6807;Parent=Msa0450910-mRNA-1
chr3_3 exon 37400788 37400873 37400788 ID=Msa0450910-mRNA-1:exon:6806;Parent=Msa0450910-mRNA-1
chr3_3 CDS 37401646 37401856 37401646 ID=Msa0450910-mRNA-1:cds;Parent=Msa0450910-mRNA-1
chr3_3 CDS 37400788 37400873 37400788 ID=Msa0450910-mRNA-1:cds;Parent=Msa0450910-mRNA-1
Gene Sequence

>Msa0450910

ATGAAGATTGATTCTCAAGCGGCGACTTATGAATTTTATAATGAGTACAGTAAAAGAATAGGATTTGATATTCTTCGAGAATATGCAAATAAAAGCAGCGTAGATGGAGTTTTGACTTCAAGAAGATTGACATGCTTCAAAGAGGGTGTACGAGGTGTTGATAAAAGACGTCAACCAAAAGGGGAGCCTAGAGCAGAAATTAGAACGAGATCATGCATAAATCTACATGAAGATGGGGGTGAATTTTTGAATGCTTGGAATTGTTTACTTGTTGAGCATAATATATTGGAAGGGTCG

Protein sequence

>Msa0450910

MKIDSQAATYEFYNEYSKRIGFDILREYANKSSVDGVLTSRRLTCFKEGVRGVDKRRQPKGEPRAEIRTRSCINLHEDGGEFLNAWNCLLVEHNILEGS