AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0456150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0456150 MtrunA17_Chr3g0103181 77.778 270 37 3 8 259 15 279 1.52e-146 411
Msa0456150 MtrunA17_Chr3g0103171 66.284 261 69 2 18 259 5 265 3.49e-116 333
Msa0456150 MtrunA17_Chr3g0103201 71.296 216 61 1 45 259 5 220 1.35e-111 320
Msa0456150 MtrunA17_Chr3g0103191 66.376 229 57 4 16 225 23 250 4.71e-101 295
Msa0456150 MtrunA17_Chr3g0103151 68.750 144 28 1 8 134 15 158 5.64e-63 194
Msa0456150 MtrunA17_Chr3g0103161 67.769 121 39 0 138 258 2 122 2.28e-53 169
Msa0456150 MtrunA17_Chr1g0163071 30.137 292 146 9 19 259 2 286 1.92e-33 123
Msa0456150 MtrunA17_Chr8g0390841 31.293 294 135 12 17 259 53 330 2.52e-30 118
Msa0456150 MtrunA17_Chr8g0390841 47.692 65 33 1 195 259 454 517 4.83e-11 62.4
Msa0456150 MtrunA17_Chr1g0208771 29.010 293 142 10 18 259 12 289 1.68e-28 112
Msa0456150 MtrunA17_Chr1g0208771 35.965 114 68 3 150 259 345 457 2.55e-11 63.2
Msa0456150 MtrunA17_Chr7g0233561 27.046 281 166 8 17 259 32 311 1.21e-23 97.4
Msa0456150 MtrunA17_Chr1g0163251 28.044 271 157 7 16 258 18 278 1.44e-21 92.8
Msa0456150 MtrunA17_Chr3g0131891 27.473 273 167 7 16 259 32 302 4.75e-20 87.8
Msa0456150 MtrunA17_Chr1g0163131 30.662 287 137 12 17 256 32 303 6.19e-19 84.3
Msa0456150 MtrunA17_Chr7g0233521 28.261 276 148 9 19 259 2 262 9.78e-19 83.2
Msa0456150 MtrunA17_Chr7g0233541 26.829 287 155 10 19 259 2 279 2.05e-17 80.1
Msa0456150 MtrunA17_Chr1g0163181 27.491 291 146 12 16 258 18 291 3.21e-16 77.8
Msa0456150 MtrunA17_Chr5g0429931 24.528 265 144 7 18 259 10 241 1.07e-15 74.3
Msa0456150 MtrunA17_Chr4g0067431 25.347 288 167 12 16 259 28 311 2.35e-14 71.6
Msa0456150 MtrunA17_Chr7g0233531 26.415 265 167 7 19 259 2 262 5.90e-14 70.1
Msa0456150 MtrunA17_Chr3g0077421 28.251 223 111 7 44 259 15 195 6.45e-14 68.6
Msa0456150 MtrunA17_Chr3g0077441 28.251 223 111 7 44 259 15 195 6.45e-14 68.6
Msa0456150 MtrunA17_Chr1g0163231 25.461 271 159 9 16 258 22 277 1.08e-13 69.3
Msa0456150 MtrunA17_Chr3g0077451 26.718 262 135 7 17 259 10 233 1.19e-13 68.6
Msa0456150 MtrunA17_Chr1g0154131 25.091 275 169 8 16 256 195 466 1.84e-13 69.7
Msa0456150 MtrunA17_Chr7g0233571 28.934 197 105 7 16 179 23 217 1.98e-13 67.8
Msa0456150 MtrunA17_Chr5g0419201 29.851 134 75 2 19 133 32 165 6.25e-13 65.9
Msa0456150 MtrunA17_Chr1g0163261 24.324 259 177 6 16 256 22 279 1.31e-12 66.2
Msa0456150 MtrunA17_Chr1g0163201 25.806 279 156 12 18 258 18 283 1.84e-12 66.6
Msa0456150 MtrunA17_Chr1g0154161 25.373 268 180 9 6 259 45 306 1.90e-12 66.2
Msa0456150 MtrunA17_Chr3g0077431 27.523 218 118 6 44 259 15 194 4.87e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 55 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATAATCCCTTTAAAATT+TGG 0.159329 3_3:+49561818 None:intergenic
TGATGAATCTGTTGAGATTT+TGG 0.170003 3_3:-49563851 Msa0456150:CDS
AGTCTATTTCTATTGCATTC+TGG 0.275475 3_3:+49563926 None:intergenic
CTCTCTTGGTCATGGCTGTT+TGG 0.290689 3_3:-49563998 Msa0456150:CDS
AGCCTCCTGATGGAACTAAA+TGG 0.321321 3_3:-49564041 Msa0456150:CDS
AAATAACTTCATCATTGAAT+TGG 0.339370 3_3:-49562026 Msa0456150:CDS
TCAAAGACACAAACATTTCC+TGG 0.345466 3_3:+49561659 None:intergenic
CGACCTCATAAGAAAGTTAG+AGG 0.354039 3_3:-49563775 Msa0456150:intron
GGCTGAAAGGCGGCGACCCT+TGG 0.381120 3_3:+49561718 None:intergenic
AGATGGTCCTTGTTTGCAAT+TGG 0.387314 3_3:-49561695 Msa0456150:CDS
CGATTTGCTACCAAATGGTT+AGG 0.388247 3_3:+49561928 None:intergenic
TTGGTGGTGTTTAAATATAA+AGG 0.388626 3_3:-49563979 Msa0456150:CDS
GGCTAGACAAAGATCACCTT+TGG 0.390961 3_3:-49563806 Msa0456150:CDS
TTTACTGAAAATTACTCTCT+TGG 0.397346 3_3:-49564012 Msa0456150:CDS
TCTTATAAAACCTAACCATT+TGG 0.401975 3_3:-49561938 Msa0456150:CDS
GATTTAATAATAATGACATT+AGG 0.429456 3_3:-49564554 None:intergenic
AATTGCAAACAAGGACCATC+TGG 0.446556 3_3:+49561697 None:intergenic
TATTTCCATTTAGTTCCATC+AGG 0.450375 3_3:+49564036 None:intergenic
AAGAAGGTGGTGCTTCAGAT+TGG 0.450619 3_3:-49561785 Msa0456150:CDS
TCTTGAGCTCTAGCTCCTCT+TGG 0.454656 3_3:+49562003 None:intergenic
ATACCTCTAACTTTCTTATG+AGG 0.456109 3_3:+49563772 None:intergenic
TGTTTACTTGTAAACTTATG+TGG 0.462859 3_3:+49564096 None:intergenic
AAGTTTGATGTACACATACT+TGG 0.463208 3_3:-49563949 Msa0456150:CDS
TATTGAGAACAAGGAGAAGA+AGG 0.473327 3_3:-49561801 Msa0456150:CDS
AGAAGGTGGTGCTTCAGATT+GGG 0.488232 3_3:-49561784 Msa0456150:CDS
TCTTGGTCATGGCTGTTTGG+TGG 0.489270 3_3:-49563995 Msa0456150:CDS
CTAACCATTTGGTAGCAAAT+CGG 0.490544 3_3:-49561927 Msa0456150:CDS
TCCTTGTTTGCAATTGGAAG+TGG 0.499889 3_3:-49561689 Msa0456150:CDS
TAAAGGGATTATTGAGAACA+AGG 0.519615 3_3:-49561810 Msa0456150:CDS
CCCTTGGTGTGCTTGTAACA+AGG 0.520082 3_3:+49561734 None:intergenic
CCAGCCGATTTGCTACCAAA+TGG 0.524445 3_3:+49561923 None:intergenic
TCCACTTCCAATTGCAAACA+AGG 0.530279 3_3:+49561688 None:intergenic
TTTACAAGTAAACATGGAGT+TGG 0.530875 3_3:-49564087 Msa0456150:CDS
GAAAATTACTCTCTTGGTCA+TGG 0.536626 3_3:-49564006 Msa0456150:CDS
CTAACTTTCTTATGAGGTCG+AGG 0.536777 3_3:+49563778 None:intergenic
CATAAGTTTACAAGTAAACA+TGG 0.547242 3_3:-49564093 Msa0456150:CDS
TCAGGAGGCTTTATCAACAC+TGG 0.549585 3_3:+49564054 None:intergenic
TTCAGATTGGGAAAAGATCA+TGG 0.554433 3_3:-49561772 Msa0456150:CDS
ACAAGGACCATCTGGCTGAA+AGG 0.555752 3_3:+49561705 None:intergenic
GTCGCCGCCTTTCAGCCAGA+TGG 0.565481 3_3:-49561712 Msa0456150:CDS
CCATTTGGTAGCAAATCGGC+TGG 0.571679 3_3:-49561923 Msa0456150:intron
TGAATGGCTTAATCAGAAGA+AGG 0.572164 3_3:-49563827 Msa0456150:CDS
TTCCATTTAGTTCCATCAGG+AGG 0.572752 3_3:+49564039 None:intergenic
ATTGGAAGTGGACTGCAACC+AGG 0.573985 3_3:-49561677 Msa0456150:CDS
GTGTTGATAAAGCCTCCTGA+TGG 0.584792 3_3:-49564051 Msa0456150:CDS
TCATCATAAGTATCACATGA+AGG 0.614640 3_3:+49563898 None:intergenic
AAGTTGGTCTGAGCAAGCAA+AGG 0.618188 3_3:+49564514 None:intergenic
CCTTGGTGTGCTTGTAACAA+GGG 0.624984 3_3:+49561735 None:intergenic
TGTCTTTGAACTGATAAACA+AGG 0.641859 3_3:-49561645 Msa0456150:CDS
TCATCATTGAATTGGCCAAG+AGG 0.642230 3_3:-49562018 Msa0456150:CDS
CCCTTGTTACAAGCACACCA+AGG 0.644445 3_3:-49561735 Msa0456150:CDS
CCTTGTTACAAGCACACCAA+GGG 0.649961 3_3:-49561734 Msa0456150:CDS
AGGACCATCTGGCTGAAAGG+CGG 0.653809 3_3:+49561708 None:intergenic
TGAGGTCGAGGAGAAACCAA+AGG 0.662076 3_3:+49563790 None:intergenic
TGAGAACAAGGAGAAGAAGG+TGG 0.699317 3_3:-49561798 Msa0456150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTAAAAAGTAAATTAAA+AGG + chr3_3:49561869-49561888 None:intergenic 10.0%
!! TTACACAATAAAATTATTAA+AGG - chr3_3:49562484-49562503 Msa0456150:intron 10.0%
!!! TAAAATCAATTTTATGATTT+TGG - chr3_3:49563430-49563449 Msa0456150:intron 10.0%
!!! TTTTTTTTATCGATTTTTAA+TGG + chr3_3:49563566-49563585 None:intergenic 10.0%
!!! TAAAATAAAAGAAGCTATTT+TGG + chr3_3:49562020-49562039 None:intergenic 15.0%
!! TATAAGCAAACAAAAATATT+TGG + chr3_3:49562897-49562916 None:intergenic 15.0%
!! CTTTGATCAAATAAAATAAA+CGG + chr3_3:49563102-49563121 None:intergenic 15.0%
!! ATACACAATAAATAAAAATG+TGG + chr3_3:49563138-49563157 None:intergenic 15.0%
!!! CTTATATTTTTATTCAATCA+TGG + chr3_3:49563387-49563406 None:intergenic 15.0%
!! TCATTATTATATTCAATACT+GGG + chr3_3:49563672-49563691 None:intergenic 15.0%
!!! TTTCATTTCATTTTAATCAA+TGG + chr3_3:49563791-49563810 None:intergenic 15.0%
!! ATTGATTAAAATGAAATGAA+AGG - chr3_3:49563790-49563809 Msa0456150:CDS 15.0%
!! ATATATATATATATTCCATG+AGG + chr3_3:49563975-49563994 None:intergenic 15.0%
!! TTTATAAGAATTGTGAAAAA+AGG + chr3_3:49564206-49564225 None:intergenic 15.0%
!!! CTAAAACTTTTATTTCTAGA+AGG - chr3_3:49561965-49561984 Msa0456150:CDS 20.0%
!!! TTCTTTCTTCTTATACATTT+TGG - chr3_3:49562431-49562450 Msa0456150:intron 20.0%
!! CAATAAAAAAAATTCATGCA+AGG + chr3_3:49562567-49562586 None:intergenic 20.0%
!!! CTTGCATGAATTTTTTTTAT+TGG - chr3_3:49562565-49562584 Msa0456150:intron 20.0%
!!! TTTGATTAGAAATTTTTCAG+CGG - chr3_3:49562658-49562677 Msa0456150:intron 20.0%
!!! CGTTTATTTTATTTGATCAA+AGG - chr3_3:49563100-49563119 Msa0456150:intron 20.0%
!! AATGATTACAAAACCTAAAT+TGG - chr3_3:49563191-49563210 Msa0456150:intron 20.0%
!!! TTTAGAATGAATTGAATAGT+TGG - chr3_3:49563297-49563316 Msa0456150:intron 20.0%
!! CTCATTATTATATTCAATAC+TGG + chr3_3:49563673-49563692 None:intergenic 20.0%
!! TTTGATTAAAAATAGATGGA+GGG + chr3_3:49563938-49563957 None:intergenic 20.0%
!! CTTGGAGTATATTTATAATT+TGG + chr3_3:49564045-49564064 None:intergenic 20.0%
!! AAATAACTTCATCATTGAAT+TGG - chr3_3:49564129-49564148 Msa0456150:intron 20.0%
!!! AGAATGTACCAAATTTTAAA+GGG - chr3_3:49564329-49564348 Msa0456150:intron 20.0%
!!! TCAATAATCCCTTTAAAATT+TGG + chr3_3:49564340-49564359 None:intergenic 20.0%
!!! ATGATTTTGAAGAAATGGAT+GGG + chr3_3:49561809-49561828 None:intergenic 25.0%
!!! CTATTTTGGTCATTAACAAA+AGG + chr3_3:49562006-49562025 None:intergenic 25.0%
! TGTTTACTTGTAAACTTATG+TGG + chr3_3:49562062-49562081 None:intergenic 25.0%
! CATAAGTTTACAAGTAAACA+TGG - chr3_3:49562062-49562081 Msa0456150:intron 25.0%
! TTTACTGAAAATTACTCTCT+TGG - chr3_3:49562143-49562162 Msa0456150:intron 25.0%
!!! TTGGTGGTGTTTAAATATAA+AGG - chr3_3:49562176-49562195 Msa0456150:intron 25.0%
!! ACTTAATCAGTTACTTTCAA+AGG + chr3_3:49562720-49562739 None:intergenic 25.0%
! TGTTTGTTTCACTAAACAAA+TGG + chr3_3:49562859-49562878 None:intergenic 25.0%
! TTTATTCAATCATGGTTTCA+CGG + chr3_3:49563379-49563398 None:intergenic 25.0%
!!! TGCTCTAGTATTGTTTTTTA+TGG + chr3_3:49563709-49563728 None:intergenic 25.0%
! CTCATATACATATATGAGAT+AGG + chr3_3:49563863-49563882 None:intergenic 25.0%
! CTTTGATTAAAAATAGATGG+AGG + chr3_3:49563939-49563958 None:intergenic 25.0%
!!! CTCCATCTATTTTTAATCAA+AGG - chr3_3:49563937-49563956 Msa0456150:CDS 25.0%
! AGCCTTTGATTAAAAATAGA+TGG + chr3_3:49563942-49563961 None:intergenic 25.0%
! TCTTATAAAACCTAACCATT+TGG - chr3_3:49564217-49564236 Msa0456150:intron 25.0%
!! CAGAATGTACCAAATTTTAA+AGG - chr3_3:49564328-49564347 Msa0456150:intron 25.0%
TATTTAAGCTAGCAAGCATT+GGG + chr3_3:49561689-49561708 None:intergenic 30.0%
ATATTTAAGCTAGCAAGCAT+TGG + chr3_3:49561690-49561709 None:intergenic 30.0%
!! TGATTTTGAAGAAATGGATG+GGG + chr3_3:49561808-49561827 None:intergenic 30.0%
!! GATGATTTTGAAGAAATGGA+TGG + chr3_3:49561810-49561829 None:intergenic 30.0%
TTTACAAGTAAACATGGAGT+TGG - chr3_3:49562068-49562087 Msa0456150:intron 30.0%
! TATTTCCATTTAGTTCCATC+AGG + chr3_3:49562122-49562141 None:intergenic 30.0%
AAGTTTGATGTACACATACT+TGG - chr3_3:49562206-49562225 Msa0456150:intron 30.0%
!! AGTCTATTTCTATTGCATTC+TGG + chr3_3:49562232-49562251 None:intergenic 30.0%
TCATCATAAGTATCACATGA+AGG + chr3_3:49562260-49562279 None:intergenic 30.0%
! TGATGAATCTGTTGAGATTT+TGG - chr3_3:49562304-49562323 Msa0456150:intron 30.0%
ATACCTCTAACTTTCTTATG+AGG + chr3_3:49562386-49562405 None:intergenic 30.0%
!!! TGACCTTGTAGTTTTGATAT+GGG - chr3_3:49562739-49562758 Msa0456150:intron 30.0%
CTTGCGAATCATAAAACAAA+AGG + chr3_3:49562772-49562791 None:intergenic 30.0%
! ATGATTCGCAAGCTAAATTT+TGG - chr3_3:49562780-49562799 Msa0456150:intron 30.0%
GAAACAAACATTCAATCTCA+TGG - chr3_3:49562869-49562888 Msa0456150:intron 30.0%
! GTTGGAGAATATCTTTTGTT+TGG + chr3_3:49562942-49562961 None:intergenic 30.0%
AGATAGATACTCAATTCTGT+TGG + chr3_3:49562960-49562979 None:intergenic 30.0%
TAAAATAAACGGCTGAGATT+TGG + chr3_3:49563091-49563110 None:intergenic 30.0%
GAGGATAATGTTTCCAATTT+AGG + chr3_3:49563207-49563226 None:intergenic 30.0%
AATTTGCAGACAAGAGAATA+AGG + chr3_3:49563229-49563248 None:intergenic 30.0%
! ATGATGAAAGCATAATTTCC+TGG + chr3_3:49563507-49563526 None:intergenic 30.0%
AACTCCATTGTCATGATATA+TGG - chr3_3:49563595-49563614 Msa0456150:intron 30.0%
CAAACCATATATCATGACAA+TGG + chr3_3:49563602-49563621 None:intergenic 30.0%
GATTACTCACTAAAAACCTA+CGG + chr3_3:49563763-49563782 None:intergenic 30.0%
TGAAATGAAAGGCTAAGATT+TGG - chr3_3:49563801-49563820 Msa0456150:CDS 30.0%
CTCATATATGTATATGAGCT+AGG - chr3_3:49563865-49563884 Msa0456150:CDS 30.0%
! TCATGATGCTTGATATTTTC+AGG + chr3_3:49564017-49564036 None:intergenic 30.0%
TATTCCACACATATATATGC+AGG - chr3_3:49564086-49564105 Msa0456150:CDS 30.0%
TAAAGGGATTATTGAGAACA+AGG - chr3_3:49564345-49564364 Msa0456150:intron 30.0%
TGTCTTTGAACTGATAAACA+AGG - chr3_3:49564510-49564529 Msa0456150:intron 30.0%
AATTTCAATTCCTCTAGCCA+TGG + chr3_3:49561778-49561797 None:intergenic 35.0%
!! GGAGGATGATTTTGAAGAAA+TGG + chr3_3:49561814-49561833 None:intergenic 35.0%
TCCTCCAAACTAATCTTCAA+AGG - chr3_3:49561828-49561847 Msa0456150:intron 35.0%
! CAAGCCTTTGAAGATTAGTT+TGG + chr3_3:49561835-49561854 None:intergenic 35.0%
GAAAATTACTCTCTTGGTCA+TGG - chr3_3:49562149-49562168 Msa0456150:intron 35.0%
! CTGTTGAGATTTTGGATGAA+TGG - chr3_3:49562312-49562331 Msa0456150:intron 35.0%
TGAATGGCTTAATCAGAAGA+AGG - chr3_3:49562328-49562347 Msa0456150:intron 35.0%
AAAAAATTCATGCAAGGTCC+TGG + chr3_3:49562561-49562580 None:intergenic 35.0%
!!! GTGACCTTGTAGTTTTGATA+TGG - chr3_3:49562738-49562757 Msa0456150:intron 35.0%
TTGCCCATATCAAAACTACA+AGG + chr3_3:49562745-49562764 None:intergenic 35.0%
!!! CGGTAGTTGTTTTTATCAGA+CGG + chr3_3:49562807-49562826 None:intergenic 35.0%
ATACTTGCGTGTTGAATGTA+TGG - chr3_3:49563353-49563372 Msa0456150:intron 35.0%
! TTAGCAAGTTTTCGATTACC+AGG - chr3_3:49563486-49563505 Msa0456150:intron 35.0%
TATTCAATACTGGGAAAGAG+AGG + chr3_3:49563663-49563682 None:intergenic 35.0%
AAAAAGATAGAGATCACCGT+AGG - chr3_3:49563744-49563763 Msa0456150:intron 35.0%
ATAGATGGAGGGTTAAGATT+TGG + chr3_3:49563927-49563946 None:intergenic 35.0%
AACAAAGAAGACATTACGCT+TGG + chr3_3:49564063-49564082 None:intergenic 35.0%
TGAAAAAAGGATTGCATGAG+AGG + chr3_3:49564193-49564212 None:intergenic 35.0%
! CTAACCATTTGGTAGCAAAT+CGG - chr3_3:49564228-49564247 Msa0456150:intron 35.0%
TATTGAGAACAAGGAGAAGA+AGG - chr3_3:49564354-49564373 Msa0456150:intron 35.0%
TTCAGATTGGGAAAAGATCA+TGG - chr3_3:49564383-49564402 Msa0456150:intron 35.0%
TCAAAGACACAAACATTTCC+TGG + chr3_3:49564499-49564518 None:intergenic 35.0%
CAGACCAACTTGTAAGTCAT+TGG - chr3_3:49561653-49561672 Msa0456150:CDS 40.0%
ACCAACTTGTAAGTCATTGG+AGG - chr3_3:49561656-49561675 Msa0456150:CDS 40.0%
CCAACTTGTAAGTCATTGGA+GGG - chr3_3:49561657-49561676 Msa0456150:CDS 40.0%
ACTTCAAACTCCATCATCCA+TGG - chr3_3:49561758-49561777 Msa0456150:CDS 40.0%
TTTGAAGAAATGGATGGGGA+AGG + chr3_3:49561804-49561823 None:intergenic 40.0%
!! GCCTTTGAAGATTAGTTTGG+AGG + chr3_3:49561832-49561851 None:intergenic 40.0%
! TTCCATTTAGTTCCATCAGG+AGG + chr3_3:49562119-49562138 None:intergenic 40.0%
CTAACTTTCTTATGAGGTCG+AGG + chr3_3:49562380-49562399 None:intergenic 40.0%
CGACCTCATAAGAAAGTTAG+AGG - chr3_3:49562380-49562399 Msa0456150:intron 40.0%
TTCACTAAACAAATGGCAGC+AGG + chr3_3:49562852-49562871 None:intergenic 40.0%
TTGCAGACAAGAGAATAAGG+AGG + chr3_3:49563226-49563245 None:intergenic 40.0%
CTCACCTGCATATATATGTG+TGG + chr3_3:49564093-49564112 None:intergenic 40.0%
TCATCATTGAATTGGCCAAG+AGG - chr3_3:49564137-49564156 Msa0456150:intron 40.0%
!! CGATTTGCTACCAAATGGTT+AGG + chr3_3:49564230-49564249 None:intergenic 40.0%
AATTGCAAACAAGGACCATC+TGG + chr3_3:49564461-49564480 None:intergenic 40.0%
! AGATGGTCCTTGTTTGCAAT+TGG - chr3_3:49564460-49564479 Msa0456150:intron 40.0%
! TCCTTGTTTGCAATTGGAAG+TGG - chr3_3:49564466-49564485 Msa0456150:intron 40.0%
TCCACTTCCAATTGCAAACA+AGG + chr3_3:49564470-49564489 None:intergenic 40.0%
! AAGTTGGTCTGAGCAAGCAA+AGG + chr3_3:49561644-49561663 None:intergenic 45.0%
CCCTCCAATGACTTACAAGT+TGG + chr3_3:49561660-49561679 None:intergenic 45.0%
! TGTAAGTCATTGGAGGGTAC+TGG - chr3_3:49561663-49561682 Msa0456150:CDS 45.0%
ATTCCTCTAGCCATGGATGA+TGG + chr3_3:49561771-49561790 None:intergenic 45.0%
! TCAGGAGGCTTTATCAACAC+TGG + chr3_3:49562104-49562123 None:intergenic 45.0%
! GTGTTGATAAAGCCTCCTGA+TGG - chr3_3:49562104-49562123 Msa0456150:intron 45.0%
AGCCTCCTGATGGAACTAAA+TGG - chr3_3:49562114-49562133 Msa0456150:intron 45.0%
GGCTAGACAAAGATCACCTT+TGG - chr3_3:49562349-49562368 Msa0456150:intron 45.0%
TTGTTAGATAAGCTGCCTCC+AGG - chr3_3:49562540-49562559 Msa0456150:intron 45.0%
AAATTCATGCAAGGTCCTGG+AGG + chr3_3:49562558-49562577 None:intergenic 45.0%
AACTACCGATGTTCTGACTG+CGG - chr3_3:49562819-49562838 Msa0456150:intron 45.0%
TACTGGGAAAGAGAGGTCAA+TGG + chr3_3:49563656-49563675 None:intergenic 45.0%
TCAACGAATCTGTTGACACC+TGG - chr3_3:49563826-49563845 Msa0456150:CDS 45.0%
TCAACGAATTCGTTGACACC+TGG + chr3_3:49563906-49563925 None:intergenic 45.0%
AGGCTCATACTTACACCTCA+TGG - chr3_3:49563957-49563976 Msa0456150:CDS 45.0%
TGAGAACAAGGAGAAGAAGG+TGG - chr3_3:49564357-49564376 Msa0456150:intron 45.0%
AAGAAGGTGGTGCTTCAGAT+TGG - chr3_3:49564370-49564389 Msa0456150:intron 45.0%
AGAAGGTGGTGCTTCAGATT+GGG - chr3_3:49564371-49564390 Msa0456150:intron 45.0%
! CCTTGGTGTGCTTGTAACAA+GGG + chr3_3:49564423-49564442 None:intergenic 45.0%
CCTTGTTACAAGCACACCAA+GGG - chr3_3:49564421-49564440 Msa0456150:intron 45.0%
!! ATATATATATATATATAGTT+AGG - chr3_3:49563029-49563048 Msa0456150:intron 5.0%
!! AACAATTAAATTTATTTAAA+GGG + chr3_3:49563468-49563487 None:intergenic 5.0%
!! TAACAATTAAATTTATTTAA+AGG + chr3_3:49563469-49563488 None:intergenic 5.0%
ACTCCATCATCCATGGCTAG+AGG - chr3_3:49561765-49561784 Msa0456150:CDS 50.0%
CTCTCTTGGTCATGGCTGTT+TGG - chr3_3:49562157-49562176 Msa0456150:intron 50.0%
TCTTGGTCATGGCTGTTTGG+TGG - chr3_3:49562160-49562179 Msa0456150:intron 50.0%
TGAGGTCGAGGAGAAACCAA+AGG + chr3_3:49562368-49562387 None:intergenic 50.0%
AGTTAGGATCCGTTGACACC+TGG - chr3_3:49563045-49563064 Msa0456150:intron 50.0%
TCAACGAATCCGTTGACACC+AGG + chr3_3:49563066-49563085 None:intergenic 50.0%
! CTGTTGACACCTGGTGTCAA+CGG - chr3_3:49563835-49563854 Msa0456150:CDS 50.0%
AGATAGGATCCGTTGACACC+AGG + chr3_3:49563847-49563866 None:intergenic 50.0%
! TCGTTGACACCTGGTGTCAA+CGG + chr3_3:49563893-49563912 None:intergenic 50.0%
! TCTTGAGCTCTAGCTCCTCT+TGG + chr3_3:49564155-49564174 None:intergenic 50.0%
!! CCAGCCGATTTGCTACCAAA+TGG + chr3_3:49564235-49564254 None:intergenic 50.0%
! CCATTTGGTAGCAAATCGGC+TGG - chr3_3:49564232-49564251 Msa0456150:intron 50.0%
TGGTAGCAAATCGGCTGGTA+AGG - chr3_3:49564237-49564256 Msa0456150:intron 50.0%
CCCTTGTTACAAGCACACCA+AGG - chr3_3:49564420-49564439 Msa0456150:intron 50.0%
! CCCTTGGTGTGCTTGTAACA+AGG + chr3_3:49564424-49564443 None:intergenic 50.0%
ACAAGGACCATCTGGCTGAA+AGG + chr3_3:49564453-49564472 None:intergenic 50.0%
!! ATTGGAAGTGGACTGCAACC+AGG - chr3_3:49564478-49564497 Msa0456150:intron 50.0%
CCACACCGCAGTCAGAACAT+CGG + chr3_3:49562827-49562846 None:intergenic 55.0%
CCGTTGACACCAGGTGTCAA+CGG + chr3_3:49563890-49563909 None:intergenic 55.0%
! CCGTTGACACCTGGTGTCAA+CGG - chr3_3:49563054-49563073 Msa0456150:intron 55.0%
AGCTAGGATCCGTTGACACC+AGG - chr3_3:49563881-49563900 Msa0456150:CDS 55.0%
AGGACCATCTGGCTGAAAGG+CGG + chr3_3:49564450-49564469 None:intergenic 55.0%
CCGATGTTCTGACTGCGGTG+TGG - chr3_3:49562824-49562843 Msa0456150:intron 60.0%
GTCGCCGCCTTTCAGCCAGA+TGG - chr3_3:49564443-49564462 Msa0456150:intron 65.0%
GGCTGAAAGGCGGCGACCCT+TGG + chr3_3:49564440-49564459 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 49561613 49564564 49561613 ID=Msa0456150;Name=Msa0456150
chr3_3 mRNA 49561613 49564564 49561613 ID=Msa0456150-mRNA-1;Parent=Msa0456150;Name=Msa0456150-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|0.75|1|1|4|0|259
chr3_3 exon 49564514 49564564 49564514 ID=Msa0456150-mRNA-1:exon:9389;Parent=Msa0456150-mRNA-1
chr3_3 exon 49563776 49564124 49563776 ID=Msa0456150-mRNA-1:exon:9388;Parent=Msa0456150-mRNA-1
chr3_3 exon 49561924 49562069 49561924 ID=Msa0456150-mRNA-1:exon:9387;Parent=Msa0456150-mRNA-1
chr3_3 exon 49561613 49561846 49561613 ID=Msa0456150-mRNA-1:exon:9386;Parent=Msa0456150-mRNA-1
chr3_3 CDS 49564514 49564564 49564514 ID=Msa0456150-mRNA-1:cds;Parent=Msa0456150-mRNA-1
chr3_3 CDS 49563776 49564124 49563776 ID=Msa0456150-mRNA-1:cds;Parent=Msa0456150-mRNA-1
chr3_3 CDS 49561924 49562069 49561924 ID=Msa0456150-mRNA-1:cds;Parent=Msa0456150-mRNA-1
chr3_3 CDS 49561613 49561846 49561613 ID=Msa0456150-mRNA-1:cds;Parent=Msa0456150-mRNA-1
Gene Sequence

>Msa0456150

ATGACATTAGGCAGTAGCTTGATTATAACCTTTGCTTGCTCAGACCAACTTAAAATACCACATAAGTTTACAAGTAAACATGGAGTTGGTCTATCAAATCCAGTGTTGATAAAGCCTCCTGATGGAACTAAATGGAAATATTTTACTGAAAATTACTCTCTTGGTCATGGCTGTTTGGTGGTGTTTAAATATAAAGGAGCTTCAAAGTTTGATGTACACATACTTGGCCAGAATGCAATAGAAATAGACTATGATCCTTCATGTGATACTTATGATGAGAAAAATGATATTCATCATAGTGATGATGAATCTGTTGAGATTTTGGATGAATGGCTTAATCAGAAGAAGGCTAGACAAAGATCACCTTTGGTTTCTCCTCGACCTCATAAGAAAGTTAGAGGTGAGATTAAGAAGACTGCTAAAATAACTTCATCATTGAATTGGCCAAGAGGAGCTAGAGCTCAAGAAGTAGCTGAAAATTTCCTCTCATGCAATCCTTTTTTCACAATTCTTATAAAACCTAACCATTTGGTAGCAAATCGGCTGAATGTACCAAATTTTAAAGGGATTATTGAGAACAAGGAGAAGAAGGTGGTGCTTCAGATTGGGAAAAGATCATGGAATGTGAAATTGCTCCCTTGTTACAAGCACACCAAGGGTCGCCGCCTTTCAGCCAGATGGTCCTTGTTTGCAATTGGAAGTGGACTGCAACCAGGAAATGTTTGTGTCTTTGAACTGATAAACAAGGAAGATTTAGTTTTTAAAGTTCATGTTTTCTAA
Protein sequence

>Msa0456150

MTLGSSLIITFACSDQLKIPHKFTSKHGVGLSNPVLIKPPDGTKWKYFTENYSLGHGCLVVFKYKGASKFDVHILGQNAIEIDYDPSCDTYDEKNDIHHSDDESVEILDEWLNQKKARQRSPLVSPRPHKKVRGEIKKTAKITSSLNWPRGARAQEVAENFLSCNPFFTILIKPNHLVANRLNVPNFKGIIENKEKKVVLQIGKRSWNVKLLPCYKHTKGRRLSARWSLFAIGSGLQPGNVCVFELINKEDLVFKVHVF*