AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0458360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0458360 MtrunA17_Chr3g0105711 87.912 546 60 5 1 544 1 542 0.0 921
Msa0458360 MtrunA17_Chr3g0136081 49.565 575 194 13 7 543 2 518 3.92e-166 481
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0458360 AT5G16100.2 24.916 297 179 6 267 528 153 440 2.71e-23 103

Find 111 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCAAGTCCATGGTAATTT+TGG 0.212819 3_3:-53865145 None:intergenic
GGCATTGCATTGGAATTAAA+TGG 0.228611 3_3:-53865362 None:intergenic
GGGTCCATTATTGCTATTAA+TGG 0.250205 3_3:-53865120 None:intergenic
ATTGCTATTAATGGACAAAT+TGG 0.250376 3_3:-53865111 None:intergenic
CAAAATAATAATGTTTCATT+TGG 0.253731 3_3:+53865686 Msa0458360:CDS
TTGTCCATTAATAGCAATAA+TGG 0.260366 3_3:+53865116 Msa0458360:CDS
CATGGACATGGTAGCAATAT+TGG 0.276231 3_3:+53865332 Msa0458360:CDS
TGGATTGCGGTTGCTCAAAT+TGG 0.290960 3_3:-53865087 None:intergenic
AGACGAATCATTTACTCTAA+TGG 0.306498 3_3:+53865836 Msa0458360:CDS
ATTGTTGGGCCTCCATTAAC+AGG 0.321028 3_3:-53866021 None:intergenic
TATGAAAATCTTGAGATTCA+TGG 0.321881 3_3:+53865958 Msa0458360:CDS
CAAGAGCAAGTTGGTGAGAT+TGG 0.322818 3_3:+53866871 Msa0458360:CDS
AACGTTGCACCTTCGATACT+TGG 0.325070 3_3:-53864939 None:intergenic
CGCCATGTGACACCTGTTAA+TGG 0.325291 3_3:+53866009 Msa0458360:CDS
TTGGATTGATGATATTAATA+TGG 0.329852 3_3:+53866287 Msa0458360:CDS
CACACTACTGCTGCTGATAA+TGG 0.341115 3_3:-53867510 None:intergenic
CTCTTTGCACCGGATATGAT+TGG 0.345931 3_3:+53866268 Msa0458360:CDS
CTTGAGATTCATGGAAGCTT+GGG 0.357672 3_3:+53865967 Msa0458360:CDS
CATGCTTACAGATGATATAA+TGG 0.390482 3_3:+53866834 Msa0458360:intron
GATATTAAATTGTAATTGAA+TGG 0.400636 3_3:-53865506 None:intergenic
TGATGAAATAAAGAACTTCC+TGG 0.412875 3_3:+53866173 Msa0458360:CDS
CTATTAATGGACAAATTGGT+TGG 0.419381 3_3:-53865107 None:intergenic
AGCAATACTTGAAAATCAAT+TGG 0.419584 3_3:+53867357 Msa0458360:CDS
CGATTACTTTCATCTACCTA+TGG 0.438638 3_3:+53867132 Msa0458360:CDS
TGGAATAAGTAACTATGAAG+TGG 0.449761 3_3:+53865706 Msa0458360:CDS
TGTATATATCATTTAGTGTA+GGG 0.456932 3_3:-53865013 None:intergenic
CTTCCTGGATCAGTATTGTA+TGG 0.458075 3_3:+53866188 Msa0458360:CDS
CGATTACAATTTAGTATCGA+TGG 0.464755 3_3:+53865439 Msa0458360:CDS
CAATCGTTCGATCAATTGTT+GGG 0.465946 3_3:-53866035 None:intergenic
TCTTGAGATTCATGGAAGCT+TGG 0.468339 3_3:+53865966 Msa0458360:CDS
CACCAAAATTACCATGGACT+TGG 0.468970 3_3:+53865143 Msa0458360:CDS
AGATGTGCACAATTTACATT+GGG 0.476510 3_3:+53867100 Msa0458360:CDS
TCAATCGTTCGATCAATTGT+TGG 0.476535 3_3:-53866036 None:intergenic
CAACAAAATTACCATGGACT+TGG 0.485409 3_3:+53865227 Msa0458360:CDS
GGAAGTTCTTTATTTCATCA+AGG 0.486733 3_3:-53866170 None:intergenic
TCAATTGGACAGGCAAGATC+CGG 0.489751 3_3:+53867372 Msa0458360:CDS
ATCATCAATCCAATCATATC+CGG 0.493113 3_3:-53866277 None:intergenic
ATTCTGTTGTGGCATTGCAT+TGG 0.493127 3_3:-53865372 None:intergenic
TGTATGGAACATAATTCAGC+AGG 0.494565 3_3:+53866204 Msa0458360:CDS
CCATGGCATTCAACATGGAC+GGG 0.499629 3_3:+53865469 Msa0458360:CDS
CCAATGGTTCAAAATCAACT+TGG 0.499953 3_3:+53865659 Msa0458360:CDS
CCATGTCCATGTTGAATACC+AGG 0.503325 3_3:-53865611 None:intergenic
GTAGAACAAAATTAAGAAGA+TGG 0.504860 3_3:+53867209 Msa0458360:CDS
AAGTGTTGGCACTTGATCAA+TGG 0.505339 3_3:+53865768 Msa0458360:CDS
CTAGCCGCTGCTTCTTCCAT+AGG 0.508372 3_3:-53867148 None:intergenic
CAACAAAGTAGTAATTTCAA+TGG 0.513743 3_3:+53865182 Msa0458360:CDS
AGAGTCCTTTCCCTTGTCGC+CGG 0.514274 3_3:-53867391 None:intergenic
ATGTATATATCATTTAGTGT+AGG 0.514577 3_3:-53865014 None:intergenic
AAGATGTGCACAATTTACAT+TGG 0.515263 3_3:+53867099 Msa0458360:CDS
GCATTGCATTGGAATTAAAT+GGG 0.518101 3_3:-53865361 None:intergenic
AAGAAGATGGCCACACAGAA+AGG 0.518680 3_3:+53867222 Msa0458360:CDS
GATTGATGATATTAATATGG+AGG 0.518874 3_3:+53866290 Msa0458360:CDS
TACTTGAAAATCAATTGGAC+AGG 0.519249 3_3:+53867362 Msa0458360:CDS
GGAATAAGTAACTATGAAGT+GGG 0.521918 3_3:+53865707 Msa0458360:CDS
ACCATGGCATTCAACATGGA+CGG 0.528445 3_3:+53865468 Msa0458360:CDS
GTTCCATACAATACTGATCC+AGG 0.531567 3_3:-53866191 None:intergenic
ATGTCACAACAAAATGATCC+TGG 0.543235 3_3:+53865593 Msa0458360:CDS
GATACTAAATTGTAATCGAA+TGG 0.547649 3_3:-53865434 None:intergenic
GCGACATAATTCAACGCAGT+TGG 0.549096 3_3:-53867469 None:intergenic
TCATGGTATTCAACAAGGGC+AGG 0.551808 3_3:+53865541 Msa0458360:CDS
AAGATCCGGCGACAAGGGAA+AGG 0.552910 3_3:+53867386 Msa0458360:CDS
ATATCACCAGAAAATGATCA+TGG 0.553458 3_3:+53865524 Msa0458360:CDS
CTCCATTAACAGGTGTCACA+TGG 0.555997 3_3:-53866011 None:intergenic
CCCGTCCATGTTGAATGCCA+TGG 0.556371 3_3:-53865469 None:intergenic
GACTTGGAATACTTCAACGA+GGG 0.562322 3_3:+53865159 Msa0458360:CDS
CAGGCAAGATCCGGCGACAA+GGG 0.569973 3_3:+53867381 Msa0458360:CDS
TATTATGAAACTCTTTGCAC+CGG 0.570226 3_3:+53866258 Msa0458360:CDS
TACATGAATAACAATAACAA+TGG 0.572167 3_3:+53865032 Msa0458360:CDS
AATGACCATGGCATTCAACA+TGG 0.573215 3_3:+53865464 Msa0458360:CDS
CACCAGAGTGTGGTTCAACA+TGG 0.578034 3_3:+53865314 Msa0458360:CDS
ATGCCATGGTCATTCTGTTG+TGG 0.583391 3_3:-53865383 None:intergenic
ATTGATGATATTAATATGGA+GGG 0.585774 3_3:+53866291 Msa0458360:CDS
GGTATTCAACAAGGGCAGGA+TGG 0.589069 3_3:+53865545 Msa0458360:CDS
GAGCAAGAGCAAGAGCAAGT+TGG 0.592584 3_3:+53866862 Msa0458360:CDS
TCAACAACAGACCGAAGTGT+TGG 0.597493 3_3:+53865754 Msa0458360:CDS
TTGAGATTCATGGAAGCTTG+GGG 0.597747 3_3:+53865968 Msa0458360:CDS
AATGATCATGGTATTCAACA+AGG 0.598505 3_3:+53865536 Msa0458360:CDS
GTAGAAGTATTCCAAGTCCA+TGG 0.598550 3_3:-53865238 None:intergenic
CCTGGTATTCAACATGGACA+TGG 0.598984 3_3:+53865611 Msa0458360:CDS
GTTGAAGTATTCCAAGTCCA+TGG 0.599108 3_3:-53865154 None:intergenic
ACAGGCAAGATCCGGCGACA+AGG 0.599603 3_3:+53867380 Msa0458360:CDS
ACCATGGCATTCCACATGGA+CGG 0.602432 3_3:+53865396 Msa0458360:CDS
TTGATCAAGTGCCAACACTT+CGG 0.602511 3_3:-53865765 None:intergenic
TCCGTCCATGTGGAATGCCA+TGG 0.610625 3_3:-53865397 None:intergenic
GGCATTCAACATGGACGGGG+TGG 0.611696 3_3:+53865473 Msa0458360:CDS
CTATCCCAACAAAATTACCA+TGG 0.618972 3_3:+53865221 Msa0458360:CDS
GGACTTGGAATACTTCTACA+AGG 0.619567 3_3:+53865242 Msa0458360:CDS
AATGACCATGGCATTCCACA+TGG 0.620959 3_3:+53865392 Msa0458360:CDS
AATGATCCTGGTATTCAACA+TGG 0.622879 3_3:+53865605 Msa0458360:CDS
CAACAACAAAATTCTTGCAG+TGG 0.625689 3_3:+53865800 Msa0458360:CDS
CATGGCATTCAACATGGACG+GGG 0.625970 3_3:+53865470 Msa0458360:CDS
CAAGACATGATTGATGATTG+TGG 0.634370 3_3:+53867436 Msa0458360:CDS
CATGTGACACCTGTTAATGG+AGG 0.638273 3_3:+53866012 Msa0458360:CDS
TGGACAAATTGGTTGGATTG+CGG 0.639366 3_3:-53865100 None:intergenic
TCTACCTATGGAAGAAGCAG+CGG 0.639381 3_3:+53867144 Msa0458360:CDS
GTCCATGTTGAACCACACTC+TGG 0.639765 3_3:-53865316 None:intergenic
TGCTGCTGATAATGGTGCGG+CGG 0.640455 3_3:-53867502 None:intergenic
GGACCCCACCAAAATTACCA+TGG 0.646800 3_3:+53865137 Msa0458360:CDS
GGAATACTTCTACAAGGACA+TGG 0.662894 3_3:+53865248 Msa0458360:CDS
TACTGCTGCTGATAATGGTG+CGG 0.670583 3_3:-53867505 None:intergenic
ATGATCATGGTATTCAACAA+GGG 0.672665 3_3:+53865537 Msa0458360:CDS
GTATGGAACATAATTCAGCA+GGG 0.676056 3_3:+53866205 Msa0458360:CDS
TAATTACTACTCCGTCCATG+TGG 0.676652 3_3:-53865407 None:intergenic
ATGCCACAACAGAATGACCA+TGG 0.677298 3_3:+53865380 Msa0458360:CDS
ATAAAATTACCTGCTCTGAG+AGG 0.686791 3_3:-53866912 None:intergenic
GACAAGGGAAAGGACTCTAG+AGG 0.686791 3_3:+53867396 Msa0458360:CDS
AGTGTGGTTCAACATGGACA+TGG 0.687520 3_3:+53865320 Msa0458360:CDS
CCAACAAAATCACCAGAGTG+TGG 0.694382 3_3:+53865304 Msa0458360:CDS
GTATCGATGGAAAATGACCA+TGG 0.695602 3_3:+53865452 Msa0458360:CDS
GACATGATTGATGATTGTGG+CGG 0.760229 3_3:+53867439 Msa0458360:CDS
GGACTTGGAATACTTCAACG+AGG 0.774898 3_3:+53865158 Msa0458360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTCATATTGAAAAAATTT+GGG - chr3_3:53864992-53865011 None:intergenic 15.0%
!! TTACTCATATTGAAAAAATT+TGG - chr3_3:53864993-53865012 None:intergenic 15.0%
!! GATATTAAATTGTAATTGAA+TGG - chr3_3:53865509-53865528 None:intergenic 15.0%
!! CAAAATAATAATGTTTCATT+TGG + chr3_3:53865686-53865705 Msa0458360:CDS 15.0%
!! CATATATATATCAAAATAAC+TGG - chr3_3:53865909-53865928 None:intergenic 15.0%
!!! GTTTATTTTGATTCTTATAT+AGG + chr3_3:53865928-53865947 Msa0458360:intron 15.0%
!!! TATCATAATGATTTTGATTT+AGG + chr3_3:53866703-53866722 Msa0458360:intron 15.0%
!!! ATCATAATGATTTTGATTTA+GGG + chr3_3:53866704-53866723 Msa0458360:intron 15.0%
!!! TTTTTAATGATATTATGTTG+AGG + chr3_3:53866794-53866813 Msa0458360:intron 15.0%
!!! TTTTTTGTGTGTATTATTTT+TGG + chr3_3:53867255-53867274 Msa0458360:intron 15.0%
!! TGTATATATCATTTAGTGTA+GGG - chr3_3:53865016-53865035 None:intergenic 20.0%
!! ATGTATATATCATTTAGTGT+AGG - chr3_3:53865017-53865036 None:intergenic 20.0%
!! TACATGAATAACAATAACAA+TGG + chr3_3:53865032-53865051 Msa0458360:CDS 20.0%
!!! TTTCTTGTAGTGATTAATTT+TGG - chr3_3:53865885-53865904 None:intergenic 20.0%
!!! TTGGATTGATGATATTAATA+TGG + chr3_3:53866287-53866306 Msa0458360:CDS 20.0%
!!! ATTGATGATATTAATATGGA+GGG + chr3_3:53866291-53866310 Msa0458360:CDS 20.0%
!!! CTTTTAGAAATACATTTGTT+TGG + chr3_3:53866559-53866578 Msa0458360:intron 20.0%
!! ATATTGTATGTATTGAATAG+AGG + chr3_3:53867074-53867093 Msa0458360:intron 20.0%
! ATTGAAAAAATTTGGGTCAA+TGG - chr3_3:53864985-53865004 None:intergenic 25.0%
!! ATTGGAATGATGATTTTGTT+GGG - chr3_3:53865072-53865091 None:intergenic 25.0%
!! AATTGGAATGATGATTTTGT+TGG - chr3_3:53865073-53865092 None:intergenic 25.0%
!! ATTGCTATTAATGGACAAAT+TGG - chr3_3:53865114-53865133 None:intergenic 25.0%
! TTGTCCATTAATAGCAATAA+TGG + chr3_3:53865116-53865135 Msa0458360:CDS 25.0%
! CAACAAAGTAGTAATTTCAA+TGG + chr3_3:53865182-53865201 Msa0458360:CDS 25.0%
! GATACTAAATTGTAATCGAA+TGG - chr3_3:53865437-53865456 None:intergenic 25.0%
! TATGAAAATCTTGAGATTCA+TGG + chr3_3:53865958-53865977 Msa0458360:CDS 25.0%
! ACAAATATTACACTAAGACT+TGG - chr3_3:53866069-53866088 None:intergenic 25.0%
!! GATTGATGATATTAATATGG+AGG + chr3_3:53866290-53866309 Msa0458360:CDS 25.0%
! AAACCTGAAAAATTATCGAT+AGG - chr3_3:53866318-53866337 None:intergenic 25.0%
!!! CTTTTAGAAATACACTTGTT+TGG + chr3_3:53866373-53866392 Msa0458360:intron 25.0%
!!! CTTTTAGAAATGCATTTGTT+TGG + chr3_3:53866467-53866486 Msa0458360:intron 25.0%
! GTAGAACAAAATTAAGAAGA+TGG + chr3_3:53867209-53867228 Msa0458360:CDS 25.0%
! CACGTATTAAATTAGCAAAA+TGG - chr3_3:53867296-53867315 None:intergenic 25.0%
! CGTGTCTTAAAATTAAGTAT+AGG + chr3_3:53867312-53867331 Msa0458360:intron 25.0%
! AGCAATACTTGAAAATCAAT+TGG + chr3_3:53867357-53867376 Msa0458360:CDS 25.0%
!! AAAAACAATCCAAGTATCGA+AGG + chr3_3:53864930-53864949 Msa0458360:CDS 30.0%
! TTTTGTTGGGACAAATTTGT+GGG - chr3_3:53865059-53865078 None:intergenic 30.0%
! ATTTTGTTGGGACAAATTTG+TGG - chr3_3:53865060-53865079 None:intergenic 30.0%
! CTATTAATGGACAAATTGGT+TGG - chr3_3:53865110-53865129 None:intergenic 30.0%
! AAGTCCATGGTAATTTTGTT+GGG - chr3_3:53865228-53865247 None:intergenic 30.0%
GCATTGCATTGGAATTAAAT+GGG - chr3_3:53865364-53865383 None:intergenic 30.0%
! CGATTACAATTTAGTATCGA+TGG + chr3_3:53865439-53865458 Msa0458360:CDS 30.0%
ATATCACCAGAAAATGATCA+TGG + chr3_3:53865524-53865543 Msa0458360:CDS 30.0%
! TGAATACCATGATCATTTTC+TGG - chr3_3:53865533-53865552 None:intergenic 30.0%
AATGATCATGGTATTCAACA+AGG + chr3_3:53865536-53865555 Msa0458360:CDS 30.0%
ATGATCATGGTATTCAACAA+GGG + chr3_3:53865537-53865556 Msa0458360:CDS 30.0%
!! ATTTTGTTGTGACATTGCAT+GGG - chr3_3:53865588-53865607 None:intergenic 30.0%
! TGAAACATTTTCTCTTCCAA+TGG + chr3_3:53865643-53865662 Msa0458360:CDS 30.0%
TGGAATAAGTAACTATGAAG+TGG + chr3_3:53865706-53865725 Msa0458360:CDS 30.0%
GGAATAAGTAACTATGAAGT+GGG + chr3_3:53865707-53865726 Msa0458360:CDS 30.0%
AGACGAATCATTTACTCTAA+TGG + chr3_3:53865836-53865855 Msa0458360:CDS 30.0%
!! TGTATCAGTTTTTCTGAGAA+AGG + chr3_3:53866147-53866166 Msa0458360:intron 30.0%
! GGAAGTTCTTTATTTCATCA+AGG - chr3_3:53866173-53866192 None:intergenic 30.0%
TGATGAAATAAAGAACTTCC+TGG + chr3_3:53866173-53866192 Msa0458360:CDS 30.0%
TATTATGAAACTCTTTGCAC+CGG + chr3_3:53866258-53866277 Msa0458360:CDS 30.0%
ATCATCAATCCAATCATATC+CGG - chr3_3:53866280-53866299 None:intergenic 30.0%
CATGCTTACAGATGATATAA+TGG + chr3_3:53866834-53866853 Msa0458360:intron 30.0%
TTGAACAATATATACTCGCA+AGG + chr3_3:53866949-53866968 Msa0458360:intron 30.0%
AAGATGTGCACAATTTACAT+TGG + chr3_3:53867099-53867118 Msa0458360:CDS 30.0%
AGATGTGCACAATTTACATT+GGG + chr3_3:53867100-53867119 Msa0458360:CDS 30.0%
! AATACTTTTACCTTTCTGTG+TGG - chr3_3:53867235-53867254 None:intergenic 30.0%
! TATAGGTGAAATGTCTTTTG+AGG + chr3_3:53867329-53867348 Msa0458360:intron 30.0%
TACTTGAAAATCAATTGGAC+AGG + chr3_3:53867362-53867381 Msa0458360:CDS 30.0%
! GGGTCCATTATTGCTATTAA+TGG - chr3_3:53865123-53865142 None:intergenic 35.0%
!! AAGTCCATGGTAATTTTGGT+GGG - chr3_3:53865144-53865163 None:intergenic 35.0%
! TTCCAAGTCCATGGTAATTT+TGG - chr3_3:53865148-53865167 None:intergenic 35.0%
!!! ATTTTGTTGGGATAGTGGAT+TGG - chr3_3:53865216-53865235 None:intergenic 35.0%
! TGGTAATTTTGTTGGGATAG+TGG - chr3_3:53865221-53865240 None:intergenic 35.0%
CTATCCCAACAAAATTACCA+TGG + chr3_3:53865221-53865240 Msa0458360:CDS 35.0%
! CAAGTCCATGGTAATTTTGT+TGG - chr3_3:53865229-53865248 None:intergenic 35.0%
CAACAAAATTACCATGGACT+TGG + chr3_3:53865227-53865246 Msa0458360:CDS 35.0%
GGCATTGCATTGGAATTAAA+TGG - chr3_3:53865365-53865384 None:intergenic 35.0%
!! CATTTTGTTGTGACATTGCA+TGG - chr3_3:53865589-53865608 None:intergenic 35.0%
ATGTCACAACAAAATGATCC+TGG + chr3_3:53865593-53865612 Msa0458360:CDS 35.0%
AATGATCCTGGTATTCAACA+TGG + chr3_3:53865605-53865624 Msa0458360:CDS 35.0%
!!! CCAAGTTGATTTTGAACCAT+TGG - chr3_3:53865662-53865681 None:intergenic 35.0%
CCAATGGTTCAAAATCAACT+TGG + chr3_3:53865659-53865678 Msa0458360:CDS 35.0%
!!! GAATTTTGTTGTTGTTCGAG+TGG - chr3_3:53865794-53865813 None:intergenic 35.0%
CAACAACAAAATTCTTGCAG+TGG + chr3_3:53865800-53865819 Msa0458360:CDS 35.0%
CAATCGTTCGATCAATTGTT+GGG - chr3_3:53866038-53866057 None:intergenic 35.0%
TCAATCGTTCGATCAATTGT+TGG - chr3_3:53866039-53866058 None:intergenic 35.0%
TGTATGGAACATAATTCAGC+AGG + chr3_3:53866204-53866223 Msa0458360:CDS 35.0%
GTATGGAACATAATTCAGCA+GGG + chr3_3:53866205-53866224 Msa0458360:CDS 35.0%
! GGTCCTATCGATAATTTTTC+AGG + chr3_3:53866312-53866331 Msa0458360:CDS 35.0%
GTTTGTCTTCGAAATCATGA+AGG + chr3_3:53866334-53866353 Msa0458360:intron 35.0%
AGATTGGAACACCTGAAAAA+AGG + chr3_3:53866887-53866906 Msa0458360:CDS 35.0%
! ATAAAATTACCTGCTCTGAG+AGG - chr3_3:53866915-53866934 None:intergenic 35.0%
CGATTACTTTCATCTACCTA+TGG + chr3_3:53867132-53867151 Msa0458360:CDS 35.0%
! CAAGACATGATTGATGATTG+TGG + chr3_3:53867436-53867455 Msa0458360:CDS 35.0%
TGGACAAATTGGTTGGATTG+CGG - chr3_3:53865103-53865122 None:intergenic 40.0%
!! AGTCCATGGTAATTTTGGTG+GGG - chr3_3:53865143-53865162 None:intergenic 40.0%
! CAAGTCCATGGTAATTTTGG+TGG - chr3_3:53865145-53865164 None:intergenic 40.0%
CACCAAAATTACCATGGACT+TGG + chr3_3:53865143-53865162 Msa0458360:CDS 40.0%
! GTTGAAGTATTCCAAGTCCA+TGG - chr3_3:53865157-53865176 None:intergenic 40.0%
GACTTGGAATACTTCAACGA+GGG + chr3_3:53865159-53865178 Msa0458360:CDS 40.0%
!! GTAGAAGTATTCCAAGTCCA+TGG - chr3_3:53865241-53865260 None:intergenic 40.0%
GGACTTGGAATACTTCTACA+AGG + chr3_3:53865242-53865261 Msa0458360:CDS 40.0%
GGAATACTTCTACAAGGACA+TGG + chr3_3:53865248-53865267 Msa0458360:CDS 40.0%
! CACACTCTGGTGATTTTGTT+GGG - chr3_3:53865306-53865325 None:intergenic 40.0%
CATGGACATGGTAGCAATAT+TGG + chr3_3:53865332-53865351 Msa0458360:CDS 40.0%
ATTCTGTTGTGGCATTGCAT+TGG - chr3_3:53865375-53865394 None:intergenic 40.0%
TAATTACTACTCCGTCCATG+TGG - chr3_3:53865410-53865429 None:intergenic 40.0%
! GTATCGATGGAAAATGACCA+TGG + chr3_3:53865452-53865471 Msa0458360:CDS 40.0%
AATGACCATGGCATTCAACA+TGG + chr3_3:53865464-53865483 Msa0458360:CDS 40.0%
TTGATCAAGTGCCAACACTT+CGG - chr3_3:53865768-53865787 None:intergenic 40.0%
!! AAGTGTTGGCACTTGATCAA+TGG + chr3_3:53865768-53865787 Msa0458360:CDS 40.0%
TCTTGAGATTCATGGAAGCT+TGG + chr3_3:53865966-53865985 Msa0458360:CDS 40.0%
CTTGAGATTCATGGAAGCTT+GGG + chr3_3:53865967-53865986 Msa0458360:CDS 40.0%
TTGAGATTCATGGAAGCTTG+GGG + chr3_3:53865968-53865987 Msa0458360:CDS 40.0%
CTTCCTGGATCAGTATTGTA+TGG + chr3_3:53866188-53866207 Msa0458360:CDS 40.0%
GTTCCATACAATACTGATCC+AGG - chr3_3:53866194-53866213 None:intergenic 40.0%
GGGTTGTGACTAAGTATTTC+TGG + chr3_3:53866724-53866743 Msa0458360:intron 40.0%
!!! TCTGAGAGGTTCCTTTTTTC+AGG - chr3_3:53866901-53866920 None:intergenic 40.0%
! GACATGATTGATGATTGTGG+CGG + chr3_3:53867439-53867458 Msa0458360:CDS 40.0%
AACGTTGCACCTTCGATACT+TGG - chr3_3:53864942-53864961 None:intergenic 45.0%
TGGATTGCGGTTGCTCAAAT+TGG - chr3_3:53865090-53865109 None:intergenic 45.0%
GGACTTGGAATACTTCAACG+AGG + chr3_3:53865158-53865177 Msa0458360:CDS 45.0%
! CCACACTCTGGTGATTTTGT+TGG - chr3_3:53865307-53865326 None:intergenic 45.0%
CCAACAAAATCACCAGAGTG+TGG + chr3_3:53865304-53865323 Msa0458360:CDS 45.0%
AGTGTGGTTCAACATGGACA+TGG + chr3_3:53865320-53865339 Msa0458360:CDS 45.0%
ATGCCACAACAGAATGACCA+TGG + chr3_3:53865380-53865399 Msa0458360:CDS 45.0%
ATGCCATGGTCATTCTGTTG+TGG - chr3_3:53865386-53865405 None:intergenic 45.0%
AATGACCATGGCATTCCACA+TGG + chr3_3:53865392-53865411 Msa0458360:CDS 45.0%
ACCATGGCATTCAACATGGA+CGG + chr3_3:53865468-53865487 Msa0458360:CDS 45.0%
TCATGGTATTCAACAAGGGC+AGG + chr3_3:53865541-53865560 Msa0458360:CDS 45.0%
CCATGTCCATGTTGAATACC+AGG - chr3_3:53865614-53865633 None:intergenic 45.0%
CCTGGTATTCAACATGGACA+TGG + chr3_3:53865611-53865630 Msa0458360:CDS 45.0%
TCAACAACAGACCGAAGTGT+TGG + chr3_3:53865754-53865773 Msa0458360:CDS 45.0%
! CTCCATTAACAGGTGTCACA+TGG - chr3_3:53866014-53866033 None:intergenic 45.0%
CATGTGACACCTGTTAATGG+AGG + chr3_3:53866012-53866031 Msa0458360:CDS 45.0%
ATTGTTGGGCCTCCATTAAC+AGG - chr3_3:53866024-53866043 None:intergenic 45.0%
CTCTTTGCACCGGATATGAT+TGG + chr3_3:53866268-53866287 Msa0458360:CDS 45.0%
!! CAAGAGCAAGTTGGTGAGAT+TGG + chr3_3:53866871-53866890 Msa0458360:CDS 45.0%
AAAAAGGAACCTCTCAGAGC+AGG + chr3_3:53866903-53866922 Msa0458360:CDS 45.0%
TCTACCTATGGAAGAAGCAG+CGG + chr3_3:53867144-53867163 Msa0458360:CDS 45.0%
AAGAAGATGGCCACACAGAA+AGG + chr3_3:53867222-53867241 Msa0458360:CDS 45.0%
TCAATTGGACAGGCAAGATC+CGG + chr3_3:53867372-53867391 Msa0458360:CDS 45.0%
GCGACATAATTCAACGCAGT+TGG - chr3_3:53867472-53867491 None:intergenic 45.0%
!! TACTGCTGCTGATAATGGTG+CGG - chr3_3:53867508-53867527 None:intergenic 45.0%
GGACCCCACCAAAATTACCA+TGG + chr3_3:53865137-53865156 Msa0458360:CDS 50.0%
CACCAGAGTGTGGTTCAACA+TGG + chr3_3:53865314-53865333 Msa0458360:CDS 50.0%
GTCCATGTTGAACCACACTC+TGG - chr3_3:53865319-53865338 None:intergenic 50.0%
ACCATGGCATTCCACATGGA+CGG + chr3_3:53865396-53865415 Msa0458360:CDS 50.0%
CCATGGCATTCAACATGGAC+GGG + chr3_3:53865469-53865488 Msa0458360:CDS 50.0%
CATGGCATTCAACATGGACG+GGG + chr3_3:53865470-53865489 Msa0458360:CDS 50.0%
GGTATTCAACAAGGGCAGGA+TGG + chr3_3:53865545-53865564 Msa0458360:CDS 50.0%
CGCCATGTGACACCTGTTAA+TGG + chr3_3:53866009-53866028 Msa0458360:CDS 50.0%
GAGCAAGAGCAAGAGCAAGT+TGG + chr3_3:53866862-53866881 Msa0458360:CDS 50.0%
AAGCGACAGACTTGTCAGCA+TGG + chr3_3:53867012-53867031 Msa0458360:intron 50.0%
GACAAGGGAAAGGACTCTAG+AGG + chr3_3:53867396-53867415 Msa0458360:CDS 50.0%
TCCGTCCATGTGGAATGCCA+TGG - chr3_3:53865400-53865419 None:intergenic 55.0%
CCCGTCCATGTTGAATGCCA+TGG - chr3_3:53865472-53865491 None:intergenic 55.0%
CTAGCCGCTGCTTCTTCCAT+AGG - chr3_3:53867151-53867170 None:intergenic 55.0%
AAGATCCGGCGACAAGGGAA+AGG + chr3_3:53867386-53867405 Msa0458360:CDS 55.0%
AGAGTCCTTTCCCTTGTCGC+CGG - chr3_3:53867394-53867413 None:intergenic 55.0%
!! TGCTGCTGATAATGGTGCGG+CGG - chr3_3:53867505-53867524 None:intergenic 55.0%
GGCATTCAACATGGACGGGG+TGG + chr3_3:53865473-53865492 Msa0458360:CDS 60.0%
ACAGGCAAGATCCGGCGACA+AGG + chr3_3:53867380-53867399 Msa0458360:CDS 60.0%
CAGGCAAGATCCGGCGACAA+GGG + chr3_3:53867381-53867400 Msa0458360:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 53864918 53867528 53864918 ID=Msa0458360;Name=Msa0458360
chr3_3 mRNA 53864918 53867528 53864918 ID=Msa0458360-mRNA-1;Parent=Msa0458360;Name=Msa0458360-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|6|0|550
chr3_3 exon 53864918 53865857 53864918 ID=Msa0458360-mRNA-1:exon:10585;Parent=Msa0458360-mRNA-1
chr3_3 exon 53865950 53866058 53865950 ID=Msa0458360-mRNA-1:exon:10586;Parent=Msa0458360-mRNA-1
chr3_3 exon 53866155 53866333 53866155 ID=Msa0458360-mRNA-1:exon:10587;Parent=Msa0458360-mRNA-1
chr3_3 exon 53866845 53866924 53866845 ID=Msa0458360-mRNA-1:exon:10588;Parent=Msa0458360-mRNA-1
chr3_3 exon 53867094 53867243 53867094 ID=Msa0458360-mRNA-1:exon:10589;Parent=Msa0458360-mRNA-1
chr3_3 exon 53867334 53867528 53867334 ID=Msa0458360-mRNA-1:exon:10590;Parent=Msa0458360-mRNA-1
chr3_3 CDS 53864918 53865857 53864918 ID=Msa0458360-mRNA-1:cds;Parent=Msa0458360-mRNA-1
chr3_3 CDS 53865950 53866058 53865950 ID=Msa0458360-mRNA-1:cds;Parent=Msa0458360-mRNA-1
chr3_3 CDS 53866155 53866333 53866155 ID=Msa0458360-mRNA-1:cds;Parent=Msa0458360-mRNA-1
chr3_3 CDS 53866845 53866924 53866845 ID=Msa0458360-mRNA-1:cds;Parent=Msa0458360-mRNA-1
chr3_3 CDS 53867094 53867243 53867094 ID=Msa0458360-mRNA-1:cds;Parent=Msa0458360-mRNA-1
chr3_3 CDS 53867334 53867528 53867334 ID=Msa0458360-mRNA-1:cds;Parent=Msa0458360-mRNA-1
Gene Sequence

>Msa0458360

ATGGCAAACAGAAAAAACAATCCAAGTATCGAAGGTGCAACGTTTAAACTTAATGATGATGATGCCATTGACCCAAATTTTTTCAATATGAGTAACCCTACACTAAATGATATATACATGAATAACAATAACAATGGACCCACAAATTTGTCCCAACAAAATCATCATTCCAATTTGAGCAACCGCAATCCAACCAATTTGTCCATTAATAGCAATAATGGACCCCACCAAAATTACCATGGACTTGGAATACTTCAACGAGGGCAACAAAGTAGTAATTTCAATGGTCACTTTTCCAATCCACTATCCCAACAAAATTACCATGGACTTGGAATACTTCTACAAGGACATGGTAGTAGTAATGAACAATTTCATACTACACTATCCCAACAAAATCACCAGAGTGTGGTTCAACATGGACATGGTAGCAATATTGGTAATGTCCCATTTAATTCCAATGCAATGCCACAACAGAATGACCATGGCATTCCACATGGACGGAGTAGTAATTACGATCCATTCGATTACAATTTAGTATCGATGGAAAATGACCATGGCATTCAACATGGACGGGGTGGTAATTATGATCCATTCAATTACAATTTAATATCACCAGAAAATGATCATGGTATTCAACAAGGGCAGGATGGTAATAATGATGTATTTTCCCATGCAATGTCACAACAAAATGATCCTGGTATTCAACATGGACATGGTAGTACAAATGAAACATTTTCTCTTCCAATGGTTCAAAATCAACTTGGTCAGCAAAATAATAATGTTTCATTTGGAATAAGTAACTATGAAGTGGGTAGTTCGTCGCGAATGAATGAGAATCAACAACAGACCGAAGTGTTGGCACTTGATCAATGGCCACTCGAACAACAACAAAATTCTTGCAGTGGTAGTCAAGTTCTTAGACGAATCATTTACTCTAATGGTTTTCAATATGAAAATCTTGAGATTCATGGAAGCTTGGGGTTGATTACACATGCAGTTCGCCATGTGACACCTGTTAATGGAGGCCCAACAATTGATCGAACGATTGATTTTTCTGAGAAAGGCCTTGATGAAATAAAGAACTTCCTGGATCAGTATTGTATGGAACATAATTCAGCAGGGTACTTTCTTATACAAGATTCAATGTGTGCGTATTATGAAACTCTTTGCACCGGATATGATTGGATTGATGATATTAATATGGAGGGTCCTATCGATAATTTTTCAGATGATATAATGGAGCAAGAGCAAGAGCAAGAGCAAGTTGGTGAGATTGGAACACCTGAAAAAAGGAACCTCTCAGAGCAGAGGAAAAGATGTGCACAATTTACATTGGGTGATTTCTGCGATTACTTTCATCTACCTATGGAAGAAGCAGCGGCTAGAGTGAACATAGCTGCATCAACTCTAAAGAAAATTTGTCGTAGAACAAAATTAAGAAGATGGCCACACAGAAAGGTGAAATGTCTTTTGAGGCAAATAGCAATACTTGAAAATCAATTGGACAGGCAAGATCCGGCGACAAGGGAAAGGACTCTAGAGGAAATTAAGCAGCTTAAACAAGACATGATTGATGATTGTGGCGGACATACACCAACTGCGTTGAATTATGTCGCTAACTTCTTGCCGCCGCACCATTATCAGCAGCAGTAG
Protein sequence

>Msa0458360

MANRKNNPSIEGATFKLNDDDAIDPNFFNMSNPTLNDIYMNNNNNGPTNLSQQNHHSNLSNRNPTNLSINSNNGPHQNYHGLGILQRGQQSSNFNGHFSNPLSQQNYHGLGILLQGHGSSNEQFHTTLSQQNHQSVVQHGHGSNIGNVPFNSNAMPQQNDHGIPHGRSSNYDPFDYNLVSMENDHGIQHGRGGNYDPFNYNLISPENDHGIQQGQDGNNDVFSHAMSQQNDPGIQHGHGSTNETFSLPMVQNQLGQQNNNVSFGISNYEVGSSSRMNENQQQTEVLALDQWPLEQQQNSCSGSQVLRRIIYSNGFQYENLEIHGSLGLITHAVRHVTPVNGGPTIDRTIDFSEKGLDEIKNFLDQYCMEHNSAGYFLIQDSMCAYYETLCTGYDWIDDINMEGPIDNFSDDIMEQEQEQEQVGEIGTPEKRNLSEQRKRCAQFTLGDFCDYFHLPMEEAAARVNIAASTLKKICRRTKLRRWPHRKVKCLLRQIAILENQLDRQDPATRERTLEEIKQLKQDMIDDCGGHTPTALNYVANFLPPHHYQQQ*