AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0459240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0459240 MtrunA17_Chr3g0107381 93.665 221 8 1 1 215 1 221 4.24e-154 426
Msa0459240 MtrunA17_Chr3g0110131 53.361 238 87 4 1 215 1 237 9.09e-75 226
Msa0459240 MtrunA17_Chr8g0351751 47.984 248 91 7 1 215 1 243 4.70e-63 196
Msa0459240 MtrunA17_Chr3g0107461 52.174 161 76 1 1 160 1 161 7.64e-56 175
Msa0459240 MtrunA17_Chr4g0014321 50.641 156 76 1 1 155 1 156 2.56e-53 171
Msa0459240 MtrunA17_Chr4g0013041 50.649 154 73 2 1 153 1 152 2.92e-51 164
Msa0459240 MtrunA17_Chr4g0014031 43.172 227 113 5 1 214 1 224 6.37e-51 164
Msa0459240 MtrunA17_Chr3g0107331 47.712 153 80 0 3 155 2 154 9.09e-51 162
Msa0459240 MtrunA17_Chr4g0014121 52.866 157 73 1 1 156 29 185 5.26e-49 158
Msa0459240 MtrunA17_Chr4g0014131 53.548 155 71 1 1 154 1 155 1.09e-48 159
Msa0459240 MtrunA17_Chr5g0432691 43.125 160 91 0 1 160 1 160 5.82e-47 152
Msa0459240 MtrunA17_Chr2g0283941 43.956 182 96 3 1 181 1 177 9.81e-47 154
Msa0459240 MtrunA17_Chr2g0296831 59.836 122 49 0 1 122 1 122 1.01e-46 150
Msa0459240 MtrunA17_Chr4g0031421 52.414 145 64 3 29 171 1 142 1.18e-43 143
Msa0459240 MtrunA17_Chr3g0107351 41.830 153 89 0 3 155 2 154 3.64e-42 140
Msa0459240 MtrunA17_Chr5g0420351 45.455 154 83 1 1 154 1 153 4.88e-42 139
Msa0459240 MtrunA17_Chr3g0107361 41.250 160 94 0 3 162 2 161 3.51e-41 137
Msa0459240 MtrunA17_Chr3g0105791 49.306 144 70 2 29 171 1 142 5.92e-41 136
Msa0459240 MtrunA17_Chr3g0107341 39.241 158 96 0 3 160 2 159 5.23e-39 132
Msa0459240 MtrunA17_Chr2g0296861 44.937 158 87 0 1 158 1 158 4.71e-37 127
Msa0459240 MtrunA17_Chr7g0218151 47.059 153 80 1 1 153 1 152 9.99e-36 123
Msa0459240 MtrunA17_Chr1g0197261 37.662 154 95 1 1 154 1 153 1.35e-34 120
Msa0459240 MtrunA17_Chr1g0193311 45.455 154 83 1 1 154 1 153 2.44e-34 120
Msa0459240 MtrunA17_Chr4g0012761 45.752 153 82 1 1 153 1 152 4.71e-34 119
Msa0459240 MtrunA17_Chr1g0188681 42.484 153 87 1 1 153 1 152 8.88e-34 118
Msa0459240 MtrunA17_Chr1g0197461 38.158 152 93 1 3 154 2 152 2.00e-33 117
Msa0459240 MtrunA17_Chr3g0106421 40.541 148 85 2 29 173 1 148 2.23e-33 119
Msa0459240 MtrunA17_Chr1g0188661 43.791 153 85 1 1 153 1 152 3.26e-32 114
Msa0459240 MtrunA17_Chr3g0106501 46.457 127 67 1 29 154 1 127 7.85e-32 113
Msa0459240 MtrunA17_Chr1g0197321 36.129 155 97 2 1 154 1 154 4.04e-30 109
Msa0459240 MtrunA17_Chr1g0188651 43.791 153 83 2 1 153 1 150 4.53e-29 106
Msa0459240 MtrunA17_Chr4g0008701 34.146 164 104 2 1 161 1 163 1.32e-23 97.8
Msa0459240 MtrunA17_Chr7g0267601 31.098 164 109 2 1 161 1 163 3.36e-22 94.0
Msa0459240 MtrunA17_Chr3g0128721 28.947 152 108 0 3 154 6 157 5.07e-20 87.8
Msa0459240 MtrunA17_Chr6g0457851 25.786 159 104 5 1 153 11 161 1.45e-15 74.3
Msa0459240 MtrunA17_Chr2g0296851 48.193 83 42 1 74 155 1 83 2.50e-15 68.9
Msa0459240 MtrunA17_Chr1g0212151 28.369 141 87 5 1 135 1 133 9.70e-15 72.0
Msa0459240 MtrunA17_Chr6g0449851 25.333 225 140 9 1 215 1 207 1.38e-13 68.2
Msa0459240 MtrunA17_Chr4g0022641 28.676 136 91 4 1 131 1 135 3.10e-13 67.8
Msa0459240 MtrunA17_Chr4g0059631 30.147 136 89 4 1 131 1 135 1.14e-12 65.1
Msa0459240 MtrunA17_Chr6g0449861 29.204 113 73 3 1 107 9 120 1.18e-12 65.9
Msa0459240 MtrunA17_Chr1g0212471 25.862 174 114 3 1 168 1 165 1.39e-11 61.2
Msa0459240 MtrunA17_Chr4g0061691 29.586 169 101 4 1 165 1 155 5.12e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0459240 AT5G48670.1 52.000 175 82 1 1 173 1 175 5.37e-54 176
Msa0459240 AT3G05860.2 39.801 201 113 3 1 195 1 199 2.89e-41 139
Msa0459240 AT3G05860.1 40.838 191 105 3 1 185 1 189 3.26e-40 138
Msa0459240 AT3G05860.3 41.304 184 100 3 1 178 1 182 2.04e-39 136
Msa0459240 AT1G65300.1 40.789 152 90 0 3 154 2 153 1.10e-37 132
Msa0459240 AT5G26650.1 40.397 151 90 0 4 154 2 152 1.85e-35 129
Msa0459240 AT5G27810.1 60.215 93 37 0 29 121 1 93 1.86e-35 122
Msa0459240 AT1G65330.1 39.474 152 92 0 3 154 2 153 5.35e-35 125
Msa0459240 AT1G31630.1 38.816 152 90 3 3 153 2 151 6.24e-34 124
Msa0459240 AT5G27960.1 38.411 151 93 0 4 154 2 152 4.83e-33 121
Msa0459240 AT1G31640.1 40.000 150 89 1 3 152 2 150 5.16e-33 124
Msa0459240 AT5G26630.1 40.909 154 91 0 1 154 1 154 7.24e-33 118
Msa0459240 AT2G28700.1 36.667 150 93 2 1 149 1 149 1.42e-28 109
Msa0459240 AT1G22590.2 36.025 161 94 2 1 158 1 155 3.95e-26 99.4
Msa0459240 AT5G06500.1 29.714 175 119 2 1 175 1 171 6.84e-23 92.8
Msa0459240 AT5G26580.1 33.117 154 89 1 1 154 1 140 6.95e-23 94.7
Msa0459240 AT2G40210.1 42.188 128 74 0 1 128 1 128 2.14e-22 94.0
Msa0459240 AT2G15660.1 29.268 123 80 2 38 157 31 149 3.41e-12 64.3
Msa0459240 AT5G55690.1 35.714 98 56 2 1 91 1 98 1.93e-11 62.4
Msa0459240 AT5G55690.2 35.714 98 56 2 1 91 1 98 1.93e-11 62.4

Find 57 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATTGTGCCACATGGTTTC+TGG 0.169034 3_3:-55692730 Msa0459240:CDS
TCCCCTCCTCTTTGTGTCAC+TGG 0.360448 3_3:+55693980 None:intergenic
CTCCAGTCACATTTCCAGCT+TGG 0.368302 3_3:+55692858 None:intergenic
TCAATGGTTGAATTTATGTT+TGG 0.390036 3_3:-55692639 Msa0459240:three_prime_UTR
AAATGTGACTGGAGGGGAGC+AGG 0.397749 3_3:-55692849 Msa0459240:CDS
TATGAGTCTCTATATTTCAA+TGG 0.409864 3_3:-55692655 Msa0459240:three_prime_UTR
TTCAAGAGACTTGTTGATCA+AGG 0.420086 3_3:+55692707 None:intergenic
AACAAAGGAATGACAACAAA+AGG 0.420798 3_3:-55693042 Msa0459240:CDS
GGCCAATGCTGCCTTTGCAT+TGG 0.439467 3_3:+55692806 None:intergenic
ACGATCCTCAACCAGAGGTT+TGG 0.446002 3_3:-55693198 Msa0459240:CDS
AGAGGTTTGGCCTTCGCCAT+GGG 0.456027 3_3:-55693185 Msa0459240:CDS
TTCGCCATGGGGAGTGCAAA+AGG 0.457021 3_3:-55693173 Msa0459240:CDS
GACTGGAGGGGAGCAGGGAA+TGG 0.473606 3_3:-55692843 Msa0459240:CDS
ATCCAATGCAAAGGCAGCAT+TGG 0.475495 3_3:-55692808 Msa0459240:CDS
GTTCTAGTTCAGGCAATCTC+CGG 0.478594 3_3:+55693137 None:intergenic
CATGGCGAAGGCCAAACCTC+TGG 0.486204 3_3:+55693187 None:intergenic
AACATCACCAATGTTGTTGT+TGG 0.488085 3_3:+55692772 None:intergenic
CGATCAGTCTCAAGCTGAAG+AGG 0.491578 3_3:-55692891 Msa0459240:CDS
AATGTGTCCATGCTTAGAGT+TGG 0.496808 3_3:-55692592 Msa0459240:three_prime_UTR
CAGAGGTTTGGCCTTCGCCA+TGG 0.503800 3_3:-55693186 Msa0459240:CDS
AATGTGACTGGAGGGGAGCA+GGG 0.507720 3_3:-55692848 Msa0459240:CDS
AAAAGGTGCTGTCAAGGTTC+CGG 0.507965 3_3:-55693156 Msa0459240:CDS
CTTTGTGTCACTGGTGATGT+AGG 0.508282 3_3:+55693989 None:intergenic
AAGAGAGTTTCTTGAAGCAA+AGG 0.517056 3_3:-55693093 Msa0459240:CDS
ATGTTTCAATGCCTTAATGC+TGG 0.536723 3_3:-55693001 Msa0459240:CDS
CGCTGCCATGACTAGGAAGA+AGG 0.539960 3_3:-55694021 Msa0459240:exon
TTCTCAACGCTGCCATGACT+AGG 0.541469 3_3:-55694028 Msa0459240:exon
GATTTCACCAACAACAACAT+TGG 0.549412 3_3:-55692779 Msa0459240:CDS
ATGAAATCAGCACGCTCTGT+GGG 0.552209 3_3:-55693252 Msa0459240:CDS
AACCAAGCTGGAAATGTGAC+TGG 0.555977 3_3:-55692860 Msa0459240:CDS
AGAACTTGAAACAGATCGAT+AGG 0.558346 3_3:-55692919 Msa0459240:CDS
GAGTGCAAAAGGTGCTGTCA+AGG 0.560319 3_3:-55693162 Msa0459240:CDS
GAGGTTGTTCAAAACCAAGC+TGG 0.560495 3_3:-55692872 Msa0459240:CDS
GCTCACCTTCTTCCTAGTCA+TGG 0.576647 3_3:+55694016 None:intergenic
CACCAGTGACACAAAGAGGA+GGG 0.584600 3_3:-55693982 Msa0459240:CDS
AAGCTGGAAATGTGACTGGA+GGG 0.588063 3_3:-55692856 Msa0459240:CDS
GAAGGCCAAACCTCTGGTTG+AGG 0.594220 3_3:+55693193 None:intergenic
GCAAAGGCAGCATTGGCCAA+TGG 0.595451 3_3:-55692801 Msa0459240:CDS
TTGTTGTTGGTGAAATCCAT+TGG 0.595560 3_3:+55692785 None:intergenic
AAGAACAGCTGAAGAAACAA+AGG 0.602104 3_3:-55693057 Msa0459240:CDS
AAGGAATGACAACAAAAGGA+AGG 0.606288 3_3:-55693038 Msa0459240:CDS
TGTCACTGGTGATGTAGGCA+AGG 0.608234 3_3:+55693994 None:intergenic
AGCTGGAAATGTGACTGGAG+GGG 0.624312 3_3:-55692855 Msa0459240:CDS
GAGGTTTGGCCTTCGCCATG+GGG 0.626589 3_3:-55693184 Msa0459240:CDS
ACCAGTGACACAAAGAGGAG+GGG 0.628101 3_3:-55693981 Msa0459240:CDS
ACATCACCAGTGACACAAAG+AGG 0.631331 3_3:-55693986 Msa0459240:CDS
CCTGTGTCCAACTCTAAGCA+TGG 0.631681 3_3:+55692585 None:intergenic
CAAGCTGGAAATGTGACTGG+AGG 0.651743 3_3:-55692857 Msa0459240:CDS
ATGATCTCAATGATATCTCA+TGG 0.651968 3_3:-55692952 Msa0459240:CDS
ACATTGCTGATCCAATGCAA+AGG 0.652826 3_3:-55692817 Msa0459240:CDS
TGAGATCATCCATGCCAACA+TGG 0.658598 3_3:+55692966 None:intergenic
AATGGTAACATTGTGCCACA+TGG 0.661986 3_3:-55692737 Msa0459240:CDS
CCATGCTTAGAGTTGGACAC+AGG 0.668027 3_3:-55692585 Msa0459240:three_prime_UTR
TCAAAACGATCCTCAACCAG+AGG 0.673462 3_3:-55693203 Msa0459240:CDS
GATGAAATCAGCACGCTCTG+TGG 0.675321 3_3:-55693253 Msa0459240:CDS
TCACCAGTGACACAAAGAGG+AGG 0.677630 3_3:-55693983 Msa0459240:CDS
GATCAAGGCCAGAAACCATG+TGG 0.733784 3_3:+55692722 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAATAAAATAAAAATT+GGG + chr3_3:55693107-55693126 None:intergenic 0.0%
!! TATTTAAATAAAATAAAAAT+TGG + chr3_3:55693108-55693127 None:intergenic 0.0%
!! ATTTAAATAAAATAAAAATT+GGG + chr3_3:55693107-55693126 None:intergenic 0.0%
!! TATTTAAATAAAATAAAAAT+TGG + chr3_3:55693108-55693127 None:intergenic 0.0%
!!! TAGTTTTTATCATTTTTCAA+TGG + chr3_3:55692812-55692831 None:intergenic 15.0%
!!! TGAAATTATTTTTATCTCAT+TGG - chr3_3:55693175-55693194 Msa0459240:CDS 15.0%
!!! TAGTTTTTATCATTTTTCAA+TGG + chr3_3:55692812-55692831 None:intergenic 15.0%
!!! TGAAATTATTTTTATCTCAT+TGG - chr3_3:55693175-55693194 Msa0459240:CDS 15.0%
!! ACATTCAAGAAAAGAAAAAA+TGG - chr3_3:55692673-55692692 Msa0459240:three_prime_UTR 20.0%
!! AAATCTTTCACTTAATTTAG+TGG + chr3_3:55693056-55693075 None:intergenic 20.0%
!!! AAAGATTTCATTTTGTTGTT+TGG - chr3_3:55693068-55693087 Msa0459240:CDS 20.0%
!! ACATTCAAGAAAAGAAAAAA+TGG - chr3_3:55692673-55692692 Msa0459240:three_prime_UTR 20.0%
!! AAATCTTTCACTTAATTTAG+TGG + chr3_3:55693056-55693075 None:intergenic 20.0%
!!! AAAGATTTCATTTTGTTGTT+TGG - chr3_3:55693068-55693087 Msa0459240:CDS 20.0%
! GAGAAAAAAATGAGTAAGAA+GGG + chr3_3:55692580-55692599 None:intergenic 25.0%
! AGAGAAAAAAATGAGTAAGA+AGG + chr3_3:55692581-55692600 None:intergenic 25.0%
! AATGGTAATTACAACATGTA+TGG + chr3_3:55692794-55692813 None:intergenic 25.0%
! AGATCGATAAATTGAATCAA+TGG - chr3_3:55692887-55692906 Msa0459240:CDS 25.0%
! GATCGATAAATTGAATCAAT+GGG - chr3_3:55692888-55692907 Msa0459240:CDS 25.0%
! ATCGATAAATTGAATCAATG+GGG - chr3_3:55692889-55692908 Msa0459240:CDS 25.0%
! TTAGTGGCAAACAAATTTAT+AGG + chr3_3:55693040-55693059 None:intergenic 25.0%
!!! TTTTTATCTCATTGGTGTTT+CGG - chr3_3:55693183-55693202 Msa0459240:CDS 25.0%
! AATGATTGAAACAACAAAGT+AGG + chr3_3:55693309-55693328 None:intergenic 25.0%
!!! CTTGTTGTATTTTTTATACC+AGG - chr3_3:55693338-55693357 Msa0459240:intron 25.0%
!! GATGTTTTACCATTTGAAAA+TGG - chr3_3:55693875-55693894 Msa0459240:intron 25.0%
!! ACAATGTTACCATTTTCAAA+TGG + chr3_3:55693887-55693906 None:intergenic 25.0%
! TATGAGTCTCTATATTTCAA+TGG - chr3_3:55693975-55693994 Msa0459240:CDS 25.0%
! TCAATGGTTGAATTTATGTT+TGG - chr3_3:55693991-55694010 Msa0459240:CDS 25.0%
! GAGAAAAAAATGAGTAAGAA+GGG + chr3_3:55692580-55692599 None:intergenic 25.0%
! AGAGAAAAAAATGAGTAAGA+AGG + chr3_3:55692581-55692600 None:intergenic 25.0%
! AATGGTAATTACAACATGTA+TGG + chr3_3:55692794-55692813 None:intergenic 25.0%
! AGATCGATAAATTGAATCAA+TGG - chr3_3:55692887-55692906 Msa0459240:CDS 25.0%
! GATCGATAAATTGAATCAAT+GGG - chr3_3:55692888-55692907 Msa0459240:CDS 25.0%
! ATCGATAAATTGAATCAATG+GGG - chr3_3:55692889-55692908 Msa0459240:CDS 25.0%
! TTAGTGGCAAACAAATTTAT+AGG + chr3_3:55693040-55693059 None:intergenic 25.0%
!!! TTTTTATCTCATTGGTGTTT+CGG - chr3_3:55693183-55693202 Msa0459240:CDS 25.0%
! AATGATTGAAACAACAAAGT+AGG + chr3_3:55693309-55693328 None:intergenic 25.0%
!!! CTTGTTGTATTTTTTATACC+AGG - chr3_3:55693338-55693357 Msa0459240:intron 25.0%
!! GATGTTTTACCATTTGAAAA+TGG - chr3_3:55693875-55693894 Msa0459240:intron 25.0%
!! ACAATGTTACCATTTTCAAA+TGG + chr3_3:55693887-55693906 None:intergenic 25.0%
! TATGAGTCTCTATATTTCAA+TGG - chr3_3:55693975-55693994 Msa0459240:CDS 25.0%
! TCAATGGTTGAATTTATGTT+TGG - chr3_3:55693991-55694010 Msa0459240:CDS 25.0%
ATGCAAGATTGATATAGACA+TGG + chr3_3:55692731-55692750 None:intergenic 30.0%
!!! TTTCATTTTGTTGTTTGGCT+TGG - chr3_3:55693073-55693092 Msa0459240:CDS 30.0%
ATTTAACATGTTTGTCCCTT+TGG - chr3_3:55693129-55693148 Msa0459240:CDS 30.0%
!! TTTTATCTCATTGGTGTTTC+GGG - chr3_3:55693184-55693203 Msa0459240:CDS 30.0%
!!! TTTTTGCTTTGTTCTAGTTC+AGG + chr3_3:55693506-55693525 None:intergenic 30.0%
AACAAAGGAATGACAACAAA+AGG - chr3_3:55693588-55693607 Msa0459240:intron 30.0%
ATGATCTCAATGATATCTCA+TGG - chr3_3:55693678-55693697 Msa0459240:intron 30.0%
ATGCAAGATTGATATAGACA+TGG + chr3_3:55692731-55692750 None:intergenic 30.0%
!!! TTTCATTTTGTTGTTTGGCT+TGG - chr3_3:55693073-55693092 Msa0459240:CDS 30.0%
ATTTAACATGTTTGTCCCTT+TGG - chr3_3:55693129-55693148 Msa0459240:CDS 30.0%
!! TTTTATCTCATTGGTGTTTC+GGG - chr3_3:55693184-55693203 Msa0459240:CDS 30.0%
!!! TTTTTGCTTTGTTCTAGTTC+AGG + chr3_3:55693506-55693525 None:intergenic 30.0%
AACAAAGGAATGACAACAAA+AGG - chr3_3:55693588-55693607 Msa0459240:intron 30.0%
ATGATCTCAATGATATCTCA+TGG - chr3_3:55693678-55693697 Msa0459240:intron 30.0%
AAATTGAATCAATGGGGAGT+TGG - chr3_3:55692895-55692914 Msa0459240:CDS 35.0%
AATTGAATCAATGGGGAGTT+GGG - chr3_3:55692896-55692915 Msa0459240:CDS 35.0%
ACTTCAGTTTACGATGTTGT+GGG + chr3_3:55692943-55692962 None:intergenic 35.0%
AACTTCAGTTTACGATGTTG+TGG + chr3_3:55692944-55692963 None:intergenic 35.0%
TCAATCTTCTTGATCAAACC+TGG + chr3_3:55693359-55693378 None:intergenic 35.0%
AAGAGAGTTTCTTGAAGCAA+AGG - chr3_3:55693537-55693556 Msa0459240:intron 35.0%
AAGAACAGCTGAAGAAACAA+AGG - chr3_3:55693573-55693592 Msa0459240:intron 35.0%
AAGGAATGACAACAAAAGGA+AGG - chr3_3:55693592-55693611 Msa0459240:intron 35.0%
ATGTTTCAATGCCTTAATGC+TGG - chr3_3:55693629-55693648 Msa0459240:intron 35.0%
CAAAAATCTGACCAGCATTA+AGG + chr3_3:55693643-55693662 None:intergenic 35.0%
AGAACTTGAAACAGATCGAT+AGG - chr3_3:55693711-55693730 Msa0459240:intron 35.0%
! TTGTTGTTGGTGAAATCCAT+TGG + chr3_3:55693848-55693867 None:intergenic 35.0%
GATTTCACCAACAACAACAT+TGG - chr3_3:55693851-55693870 Msa0459240:intron 35.0%
AACATCACCAATGTTGTTGT+TGG + chr3_3:55693861-55693880 None:intergenic 35.0%
!! TTCAAGAGACTTGTTGATCA+AGG + chr3_3:55693926-55693945 None:intergenic 35.0%
AAATTGAATCAATGGGGAGT+TGG - chr3_3:55692895-55692914 Msa0459240:CDS 35.0%
AATTGAATCAATGGGGAGTT+GGG - chr3_3:55692896-55692915 Msa0459240:CDS 35.0%
ACTTCAGTTTACGATGTTGT+GGG + chr3_3:55692943-55692962 None:intergenic 35.0%
AACTTCAGTTTACGATGTTG+TGG + chr3_3:55692944-55692963 None:intergenic 35.0%
TCAATCTTCTTGATCAAACC+TGG + chr3_3:55693359-55693378 None:intergenic 35.0%
AAGAGAGTTTCTTGAAGCAA+AGG - chr3_3:55693537-55693556 Msa0459240:intron 35.0%
AAGAACAGCTGAAGAAACAA+AGG - chr3_3:55693573-55693592 Msa0459240:intron 35.0%
AAGGAATGACAACAAAAGGA+AGG - chr3_3:55693592-55693611 Msa0459240:intron 35.0%
ATGTTTCAATGCCTTAATGC+TGG - chr3_3:55693629-55693648 Msa0459240:intron 35.0%
CAAAAATCTGACCAGCATTA+AGG + chr3_3:55693643-55693662 None:intergenic 35.0%
AGAACTTGAAACAGATCGAT+AGG - chr3_3:55693711-55693730 Msa0459240:intron 35.0%
! TTGTTGTTGGTGAAATCCAT+TGG + chr3_3:55693848-55693867 None:intergenic 35.0%
GATTTCACCAACAACAACAT+TGG - chr3_3:55693851-55693870 Msa0459240:intron 35.0%
AACATCACCAATGTTGTTGT+TGG + chr3_3:55693861-55693880 None:intergenic 35.0%
!! TTCAAGAGACTTGTTGATCA+AGG + chr3_3:55693926-55693945 None:intergenic 35.0%
GTTCAAATCCCAGTTGTGAA+AGG - chr3_3:55692961-55692980 Msa0459240:CDS 40.0%
TATGAGCACCTTTCACAACT+GGG + chr3_3:55692972-55692991 None:intergenic 40.0%
ATATGAGCACCTTTCACAAC+TGG + chr3_3:55692973-55692992 None:intergenic 40.0%
! TCAGCTGTTCTTGAGCTTTT+TGG + chr3_3:55693565-55693584 None:intergenic 40.0%
! GGTCAGATTTTTGACCATGT+TGG - chr3_3:55693650-55693669 Msa0459240:intron 40.0%
! GATTTTTGACCATGTTGGCA+TGG - chr3_3:55693655-55693674 Msa0459240:intron 40.0%
ACATTGCTGATCCAATGCAA+AGG - chr3_3:55693813-55693832 Msa0459240:intron 40.0%
AATGGTAACATTGTGCCACA+TGG - chr3_3:55693893-55693912 Msa0459240:intron 40.0%
AATGTGTCCATGCTTAGAGT+TGG - chr3_3:55694038-55694057 Msa0459240:five_prime_UTR 40.0%
GTTCAAATCCCAGTTGTGAA+AGG - chr3_3:55692961-55692980 Msa0459240:CDS 40.0%
TATGAGCACCTTTCACAACT+GGG + chr3_3:55692972-55692991 None:intergenic 40.0%
ATATGAGCACCTTTCACAAC+TGG + chr3_3:55692973-55692992 None:intergenic 40.0%
! TCAGCTGTTCTTGAGCTTTT+TGG + chr3_3:55693565-55693584 None:intergenic 40.0%
! GGTCAGATTTTTGACCATGT+TGG - chr3_3:55693650-55693669 Msa0459240:intron 40.0%
! GATTTTTGACCATGTTGGCA+TGG - chr3_3:55693655-55693674 Msa0459240:intron 40.0%
ACATTGCTGATCCAATGCAA+AGG - chr3_3:55693813-55693832 Msa0459240:intron 40.0%
AATGGTAACATTGTGCCACA+TGG - chr3_3:55693893-55693912 Msa0459240:intron 40.0%
AATGTGTCCATGCTTAGAGT+TGG - chr3_3:55694038-55694057 Msa0459240:five_prime_UTR 40.0%
! CTTTGTGTCACTGGTGATGT+AGG + chr3_3:55692644-55692663 None:intergenic 45.0%
ACATCACCAGTGACACAAAG+AGG - chr3_3:55692644-55692663 Msa0459240:three_prime_UTR 45.0%
CACACAGACGCTATTCCAAA+GGG + chr3_3:55693147-55693166 None:intergenic 45.0%
TCACACAGACGCTATTCCAA+AGG + chr3_3:55693148-55693167 None:intergenic 45.0%
ATGAAATCAGCACGCTCTGT+GGG - chr3_3:55693378-55693397 Msa0459240:intron 45.0%
TCAAAACGATCCTCAACCAG+AGG - chr3_3:55693427-55693446 Msa0459240:intron 45.0%
!! AAAAGGTGCTGTCAAGGTTC+CGG - chr3_3:55693474-55693493 Msa0459240:intron 45.0%
!! GTTCTAGTTCAGGCAATCTC+CGG + chr3_3:55693496-55693515 None:intergenic 45.0%
TGAGATCATCCATGCCAACA+TGG + chr3_3:55693667-55693686 None:intergenic 45.0%
GAGGTTGTTCAAAACCAAGC+TGG - chr3_3:55693758-55693777 Msa0459240:intron 45.0%
AACCAAGCTGGAAATGTGAC+TGG - chr3_3:55693770-55693789 Msa0459240:intron 45.0%
AAGCTGGAAATGTGACTGGA+GGG - chr3_3:55693774-55693793 Msa0459240:intron 45.0%
ATCCAATGCAAAGGCAGCAT+TGG - chr3_3:55693822-55693841 Msa0459240:intron 45.0%
ACATTGTGCCACATGGTTTC+TGG - chr3_3:55693900-55693919 Msa0459240:intron 45.0%
! CTTTGTGTCACTGGTGATGT+AGG + chr3_3:55692644-55692663 None:intergenic 45.0%
ACATCACCAGTGACACAAAG+AGG - chr3_3:55692644-55692663 Msa0459240:three_prime_UTR 45.0%
CACACAGACGCTATTCCAAA+GGG + chr3_3:55693147-55693166 None:intergenic 45.0%
TCACACAGACGCTATTCCAA+AGG + chr3_3:55693148-55693167 None:intergenic 45.0%
ATGAAATCAGCACGCTCTGT+GGG - chr3_3:55693378-55693397 Msa0459240:intron 45.0%
TCAAAACGATCCTCAACCAG+AGG - chr3_3:55693427-55693446 Msa0459240:intron 45.0%
!! AAAAGGTGCTGTCAAGGTTC+CGG - chr3_3:55693474-55693493 Msa0459240:intron 45.0%
!! GTTCTAGTTCAGGCAATCTC+CGG + chr3_3:55693496-55693515 None:intergenic 45.0%
TGAGATCATCCATGCCAACA+TGG + chr3_3:55693667-55693686 None:intergenic 45.0%
GAGGTTGTTCAAAACCAAGC+TGG - chr3_3:55693758-55693777 Msa0459240:intron 45.0%
AACCAAGCTGGAAATGTGAC+TGG - chr3_3:55693770-55693789 Msa0459240:intron 45.0%
AAGCTGGAAATGTGACTGGA+GGG - chr3_3:55693774-55693793 Msa0459240:intron 45.0%
ATCCAATGCAAAGGCAGCAT+TGG - chr3_3:55693822-55693841 Msa0459240:intron 45.0%
ACATTGTGCCACATGGTTTC+TGG - chr3_3:55693900-55693919 Msa0459240:intron 45.0%
!! TTTAAATAAAATAAAAATTG+GGG + chr3_3:55693106-55693125 None:intergenic 5.0%
!! TTTAAATAAAATAAAAATTG+GGG + chr3_3:55693106-55693125 None:intergenic 5.0%
TTCTCAACGCTGCCATGACT+AGG - chr3_3:55692602-55692621 Msa0459240:three_prime_UTR 50.0%
GCTCACCTTCTTCCTAGTCA+TGG + chr3_3:55692617-55692636 None:intergenic 50.0%
! TGTCACTGGTGATGTAGGCA+AGG + chr3_3:55692639-55692658 None:intergenic 50.0%
TCACCAGTGACACAAAGAGG+AGG - chr3_3:55692647-55692666 Msa0459240:three_prime_UTR 50.0%
CACCAGTGACACAAAGAGGA+GGG - chr3_3:55692648-55692667 Msa0459240:three_prime_UTR 50.0%
ACCAGTGACACAAAGAGGAG+GGG - chr3_3:55692649-55692668 Msa0459240:three_prime_UTR 50.0%
GGGAGTTGGGTCTAGCAAAA+TGG - chr3_3:55692909-55692928 Msa0459240:CDS 50.0%
GATGAAATCAGCACGCTCTG+TGG - chr3_3:55693377-55693396 Msa0459240:intron 50.0%
ACGATCCTCAACCAGAGGTT+TGG - chr3_3:55693432-55693451 Msa0459240:intron 50.0%
!! GAGTGCAAAAGGTGCTGTCA+AGG - chr3_3:55693468-55693487 Msa0459240:intron 50.0%
CGATCAGTCTCAAGCTGAAG+AGG - chr3_3:55693739-55693758 Msa0459240:intron 50.0%
CTCCAGTCACATTTCCAGCT+TGG + chr3_3:55693775-55693794 None:intergenic 50.0%
CAAGCTGGAAATGTGACTGG+AGG - chr3_3:55693773-55693792 Msa0459240:intron 50.0%
AGCTGGAAATGTGACTGGAG+GGG - chr3_3:55693775-55693794 Msa0459240:intron 50.0%
GATCAAGGCCAGAAACCATG+TGG + chr3_3:55693911-55693930 None:intergenic 50.0%
CCTGTGTCCAACTCTAAGCA+TGG + chr3_3:55694048-55694067 None:intergenic 50.0%
CCATGCTTAGAGTTGGACAC+AGG - chr3_3:55694045-55694064 Msa0459240:five_prime_UTR 50.0%
TTCTCAACGCTGCCATGACT+AGG - chr3_3:55692602-55692621 Msa0459240:three_prime_UTR 50.0%
GCTCACCTTCTTCCTAGTCA+TGG + chr3_3:55692617-55692636 None:intergenic 50.0%
! TGTCACTGGTGATGTAGGCA+AGG + chr3_3:55692639-55692658 None:intergenic 50.0%
TCACCAGTGACACAAAGAGG+AGG - chr3_3:55692647-55692666 Msa0459240:three_prime_UTR 50.0%
CACCAGTGACACAAAGAGGA+GGG - chr3_3:55692648-55692667 Msa0459240:three_prime_UTR 50.0%
ACCAGTGACACAAAGAGGAG+GGG - chr3_3:55692649-55692668 Msa0459240:three_prime_UTR 50.0%
GGGAGTTGGGTCTAGCAAAA+TGG - chr3_3:55692909-55692928 Msa0459240:CDS 50.0%
GATGAAATCAGCACGCTCTG+TGG - chr3_3:55693377-55693396 Msa0459240:intron 50.0%
ACGATCCTCAACCAGAGGTT+TGG - chr3_3:55693432-55693451 Msa0459240:intron 50.0%
!! GAGTGCAAAAGGTGCTGTCA+AGG - chr3_3:55693468-55693487 Msa0459240:intron 50.0%
CGATCAGTCTCAAGCTGAAG+AGG - chr3_3:55693739-55693758 Msa0459240:intron 50.0%
CTCCAGTCACATTTCCAGCT+TGG + chr3_3:55693775-55693794 None:intergenic 50.0%
CAAGCTGGAAATGTGACTGG+AGG - chr3_3:55693773-55693792 Msa0459240:intron 50.0%
AGCTGGAAATGTGACTGGAG+GGG - chr3_3:55693775-55693794 Msa0459240:intron 50.0%
GATCAAGGCCAGAAACCATG+TGG + chr3_3:55693911-55693930 None:intergenic 50.0%
CCTGTGTCCAACTCTAAGCA+TGG + chr3_3:55694048-55694067 None:intergenic 50.0%
CCATGCTTAGAGTTGGACAC+AGG - chr3_3:55694045-55694064 Msa0459240:five_prime_UTR 50.0%
CGCTGCCATGACTAGGAAGA+AGG - chr3_3:55692609-55692628 Msa0459240:three_prime_UTR 55.0%
TCCCCTCCTCTTTGTGTCAC+TGG + chr3_3:55692653-55692672 None:intergenic 55.0%
GAAGGCCAAACCTCTGGTTG+AGG + chr3_3:55693440-55693459 None:intergenic 55.0%
! AGAGGTTTGGCCTTCGCCAT+GGG - chr3_3:55693445-55693464 Msa0459240:intron 55.0%
! TTTTGCACTCCCCATGGCGA+AGG + chr3_3:55693458-55693477 None:intergenic 55.0%
! TTCGCCATGGGGAGTGCAAA+AGG - chr3_3:55693457-55693476 Msa0459240:intron 55.0%
! AGCACCTTTTGCACTCCCCA+TGG + chr3_3:55693464-55693483 None:intergenic 55.0%
AAATGTGACTGGAGGGGAGC+AGG - chr3_3:55693781-55693800 Msa0459240:intron 55.0%
AATGTGACTGGAGGGGAGCA+GGG - chr3_3:55693782-55693801 Msa0459240:intron 55.0%
GGCCAATGCTGCCTTTGCAT+TGG + chr3_3:55693827-55693846 None:intergenic 55.0%
!! GCAAAGGCAGCATTGGCCAA+TGG - chr3_3:55693829-55693848 Msa0459240:intron 55.0%
CGCTGCCATGACTAGGAAGA+AGG - chr3_3:55692609-55692628 Msa0459240:three_prime_UTR 55.0%
TCCCCTCCTCTTTGTGTCAC+TGG + chr3_3:55692653-55692672 None:intergenic 55.0%
GAAGGCCAAACCTCTGGTTG+AGG + chr3_3:55693440-55693459 None:intergenic 55.0%
! AGAGGTTTGGCCTTCGCCAT+GGG - chr3_3:55693445-55693464 Msa0459240:intron 55.0%
! TTTTGCACTCCCCATGGCGA+AGG + chr3_3:55693458-55693477 None:intergenic 55.0%
! TTCGCCATGGGGAGTGCAAA+AGG - chr3_3:55693457-55693476 Msa0459240:intron 55.0%
! AGCACCTTTTGCACTCCCCA+TGG + chr3_3:55693464-55693483 None:intergenic 55.0%
AAATGTGACTGGAGGGGAGC+AGG - chr3_3:55693781-55693800 Msa0459240:intron 55.0%
AATGTGACTGGAGGGGAGCA+GGG - chr3_3:55693782-55693801 Msa0459240:intron 55.0%
GGCCAATGCTGCCTTTGCAT+TGG + chr3_3:55693827-55693846 None:intergenic 55.0%
!! GCAAAGGCAGCATTGGCCAA+TGG - chr3_3:55693829-55693848 Msa0459240:intron 55.0%
CATGGCGAAGGCCAAACCTC+TGG + chr3_3:55693446-55693465 None:intergenic 60.0%
! CAGAGGTTTGGCCTTCGCCA+TGG - chr3_3:55693444-55693463 Msa0459240:intron 60.0%
! GAGGTTTGGCCTTCGCCATG+GGG - chr3_3:55693446-55693465 Msa0459240:intron 60.0%
CATGGCGAAGGCCAAACCTC+TGG + chr3_3:55693446-55693465 None:intergenic 60.0%
! CAGAGGTTTGGCCTTCGCCA+TGG - chr3_3:55693444-55693463 Msa0459240:intron 60.0%
! GAGGTTTGGCCTTCGCCATG+GGG - chr3_3:55693446-55693465 Msa0459240:intron 60.0%
GACTGGAGGGGAGCAGGGAA+TGG - chr3_3:55693787-55693806 Msa0459240:intron 65.0%
GACTGGAGGGGAGCAGGGAA+TGG - chr3_3:55693787-55693806 Msa0459240:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 55692569 55694083 55692569 ID=Msa0459240;Name=Msa0459240
chr3_3 mRNA 55692569 55694083 55692569 ID=Msa0459240-mRNA-1;Parent=Msa0459240;Name=Msa0459240-mRNA-1;_AED=0.01;_eAED=0.01;_QI=47|1|1|1|1|1|2|155|215
chr3_3 exon 55692569 55693292 55692569 ID=Msa0459240-mRNA-1:exon:11063;Parent=Msa0459240-mRNA-1
chr3_3 exon 55693958 55694083 55693958 ID=Msa0459240-mRNA-1:exon:11062;Parent=Msa0459240-mRNA-1
chr3_3 five_prime_UTR 55694037 55694083 55694037 ID=Msa0459240-mRNA-1:five_prime_utr;Parent=Msa0459240-mRNA-1
chr3_3 CDS 55693958 55694036 55693958 ID=Msa0459240-mRNA-1:cds;Parent=Msa0459240-mRNA-1
chr3_3 CDS 55692724 55693292 55692724 ID=Msa0459240-mRNA-1:cds;Parent=Msa0459240-mRNA-1
chr3_3 three_prime_UTR 55692569 55692723 55692569 ID=Msa0459240-mRNA-1:three_prime_utr;Parent=Msa0459240-mRNA-1
Gene Sequence

>Msa0459240

ATGACTAGGAAGAAGGTGAGCCTTGCCTACATCACCAGTGACACAAAGAGGAGGGGAACATTCAAGAAAAGAAAAAATGGTTTGATCAAGAAGATTGATGAAATCAGCACGCTCTGTGGGATCGAAGCGTGTGCAATCATCTTTGATCAAAACGATCCTCAACCAGAGGTTTGGCCTTCGCCATGGGGAGTGCAAAAGGTGCTGTCAAGGTTCCGGAGATTGCCTGAACTAGAACAAAGCAAAAAAATGTTCAATCAAGAGAGTTTCTTGAAGCAAAGGATCCAAAAAGCTCAAGAACAGCTGAAGAAACAAAGGAATGACAACAAAAGGAAGGAAATGACTCATCTGATGTTTCAATGCCTTAATGCTGGTCAGATTTTTGACCATGTTGGCATGGATGATCTCAATGATATCTCATGGTTGATTGATCAGAACTTGAAACAGATCGATAGGAAACTCGATCAGTCTCAAGCTGAAGAGGTTGTTCAAAACCAAGCTGGAAATGTGACTGGAGGGGAGCAGGGAATGGAGAACATTGCTGATCCAATGCAAAGGCAGCATTGGCCAATGGATTTCACCAACAACAACATTGGTGATGTTTTACCATTTGAAAATGGTAACATTGTGCCACATGGTTTCTGGCCTTGA
Protein sequence

>Msa0459240

MTRKKVSLAYITSDTKRRGTFKKRKNGLIKKIDEISTLCGIEACAIIFDQNDPQPEVWPSPWGVQKVLSRFRRLPELEQSKKMFNQESFLKQRIQKAQEQLKKQRNDNKRKEMTHLMFQCLNAGQIFDHVGMDDLNDISWLIDQNLKQIDRKLDQSQAEEVVQNQAGNVTGGEQGMENIADPMQRQHWPMDFTNNNIGDVLPFENGNIVPHGFWP*