AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0462960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0462960 MtrunA17_Chr7g0217721 62.573 171 14 2 1 121 1 171 1.35e-64 199
Msa0462960 MtrunA17_Chr7g0217711 63.030 165 17 2 1 121 1 165 6.56e-64 192
Msa0462960 MtrunA17_Chr4g0060931 53.642 151 30 3 5 116 2 151 2.33e-43 145
Msa0462960 MtrunA17_Chr6g0453451 50.314 159 40 2 1 121 2 159 5.94e-43 143
Msa0462960 MtrunA17_Chr3g0113041 92.537 67 5 0 1 67 1 67 8.36e-42 132
Msa0462960 MtrunA17_Chr3g0091921 51.333 150 34 2 5 116 2 150 1.00e-40 137
Msa0462960 MtrunA17_Chr3g0113011 79.452 73 15 0 1 73 1 73 3.91e-38 123
Msa0462960 MtrunA17_Chr6g0484441 51.181 127 24 1 19 107 5 131 1.08e-37 129
Msa0462960 MtrunA17_Chr6g0461661 47.619 147 36 3 14 120 13 158 1.36e-37 130
Msa0462960 MtrunA17_Chr2g0316971 49.645 141 30 3 17 117 21 160 2.08e-36 127
Msa0462960 MtrunA17_Chr3g0113031 95.000 60 3 0 62 121 21 80 2.99e-36 124
Msa0462960 MtrunA17_Chr7g0253011 47.297 148 35 4 11 116 9 155 4.60e-35 124
Msa0462960 MtrunA17_Chr3g0113001 90.000 60 6 0 62 121 46 105 2.03e-33 117
Msa0462960 MtrunA17_Chr1g0147421 48.062 129 26 3 19 106 27 155 4.77e-32 115
Msa0462960 MtrunA17_Chr3g0139331 43.066 137 38 2 19 115 11 147 1.37e-30 111
Msa0462960 MtrunA17_Chr3g0144271 43.333 150 43 4 7 115 12 160 3.29e-30 111
Msa0462960 MtrunA17_Chr2g0312321 41.667 132 35 3 16 108 8 136 4.54e-27 101
Msa0462960 MtrunA17_Chr4g0056541 39.695 131 38 1 20 109 8 138 1.73e-26 100
Msa0462960 MtrunA17_Chr8g0346001 37.500 144 49 1 14 116 10 153 3.84e-26 100
Msa0462960 MtrunA17_Chr2g0309891 40.625 128 36 1 19 106 6 133 4.64e-26 99.4
Msa0462960 MtrunA17_Chr5g0398971 40.152 132 37 2 20 109 8 139 5.63e-26 99.4
Msa0462960 MtrunA17_Chr5g0433781 39.695 131 39 1 17 107 4 134 5.88e-26 100
Msa0462960 MtrunA17_Chr4g0018391 38.931 131 39 1 20 109 8 138 6.14e-26 99.4
Msa0462960 MtrunA17_Chr3g0128111 42.276 123 31 1 23 105 46 168 1.09e-25 96.7
Msa0462960 MtrunA17_Chr6g0481091 39.695 131 39 1 17 107 4 134 1.14e-25 96.7
Msa0462960 MtrunA17_Chr3g0109821 38.931 131 40 1 17 107 4 134 1.29e-25 100
Msa0462960 MtrunA17_Chr3g0128151 42.520 127 33 1 19 105 22 148 1.59e-25 99.8
Msa0462960 MtrunA17_Chr8g0372121 38.760 129 38 1 20 107 7 135 1.84e-25 97.4
Msa0462960 MtrunA17_Chr4g0017921 42.636 129 33 1 20 107 9 137 1.89e-25 98.2
Msa0462960 MtrunA17_Chr5g0405211 37.405 131 41 1 20 109 9 139 4.94e-25 97.1
Msa0462960 MtrunA17_Chr2g0309331 38.571 140 44 2 16 113 7 146 5.87e-25 96.7
Msa0462960 MtrunA17_Chr8g0359921 39.007 141 47 2 16 118 11 150 6.28e-25 96.3
Msa0462960 MtrunA17_Chr5g0417791 39.695 131 41 1 17 109 1 131 2.34e-24 93.6
Msa0462960 MtrunA17_Chr2g0318081 39.535 129 40 1 16 106 13 141 2.81e-24 94.7
Msa0462960 MtrunA17_Chr3g0124761 37.500 128 43 2 16 106 4 131 3.28e-24 93.6
Msa0462960 MtrunA17_Chr4g0042561 40.000 130 40 1 18 109 8 137 4.47e-24 92.8
Msa0462960 MtrunA17_Chr5g0428991 41.406 128 35 1 18 105 24 151 5.22e-24 95.5
Msa0462960 MtrunA17_Chr7g0214071 39.695 131 42 2 13 106 9 139 5.30e-24 92.4
Msa0462960 MtrunA17_Chr3g0130731 40.625 128 37 2 17 106 5 131 7.42e-24 92.8
Msa0462960 MtrunA17_Chr1g0183741 36.641 131 43 2 17 107 8 138 8.01e-24 92.4
Msa0462960 MtrunA17_Chr7g0263931 36.735 147 53 2 11 117 3 149 2.78e-23 92.0
Msa0462960 MtrunA17_Chr1g0200561 35.915 142 42 2 20 120 20 153 2.93e-23 92.0
Msa0462960 MtrunA17_Chr8g0384721 39.844 128 38 2 17 106 5 131 5.61e-23 90.5
Msa0462960 MtrunA17_Chr2g0311661 34.969 163 59 5 1 117 1 162 1.19e-22 89.7
Msa0462960 MtrunA17_Chr7g0261411 35.878 131 44 2 17 107 8 138 1.46e-22 89.4
Msa0462960 MtrunA17_Chr2g0318101 37.037 135 45 2 15 109 11 145 1.73e-22 90.1
Msa0462960 MtrunA17_Chr4g0047841 37.226 137 42 3 19 115 15 147 2.70e-22 89.4
Msa0462960 MtrunA17_Chr2g0311651 34.731 167 55 5 1 117 1 163 5.16e-22 88.2
Msa0462960 MtrunA17_Chr3g0130101 34.483 145 54 2 12 116 1 144 7.54e-22 88.2
Msa0462960 MtrunA17_Chr1g0197361 38.519 135 41 3 20 113 10 143 8.29e-22 89.4
Msa0462960 MtrunA17_Chr7g0252971 36.552 145 45 2 12 109 1 145 1.55e-21 87.4
Msa0462960 MtrunA17_Chr1g0197351 41.600 125 32 3 20 104 44 167 3.87e-21 87.0
Msa0462960 MtrunA17_Chr2g0326361 36.957 138 47 2 19 116 14 151 5.30e-21 85.5
Msa0462960 MtrunA17_Chr5g0417001 37.879 132 41 3 15 106 11 141 1.71e-20 85.1
Msa0462960 MtrunA17_Chr8g0364791 35.766 137 47 2 20 116 8 143 1.71e-20 85.1
Msa0462960 MtrunA17_Chr1g0201021 36.364 132 43 2 20 111 21 151 3.90e-20 84.3
Msa0462960 MtrunA17_Chr8g0384201 35.075 134 46 2 20 113 8 140 1.39e-19 82.8
Msa0462960 MtrunA17_Chr4g0054791 34.074 135 46 2 15 106 2 136 1.62e-19 81.6
Msa0462960 MtrunA17_Chr8g0385991 34.586 133 49 2 12 106 7 139 1.85e-19 80.1
Msa0462960 MtrunA17_Chr5g0400461 34.307 137 43 2 17 106 4 140 3.65e-19 80.9
Msa0462960 MtrunA17_Chr3g0092671 36.923 130 36 4 20 106 7 133 1.31e-18 80.1
Msa0462960 MtrunA17_Chr2g0321631 34.351 131 41 3 20 105 5 135 3.80e-17 74.3
Msa0462960 MtrunA17_Chr8g0345671 40.000 130 34 5 18 104 10 138 7.83e-17 74.7
Msa0462960 MtrunA17_Chr4g0038751 33.113 151 56 4 1 110 1 147 1.02e-15 71.2
Msa0462960 MtrunA17_Chr2g0321741 33.962 106 31 1 38 104 11 116 1.02e-14 68.6
Msa0462960 MtrunA17_Chr7g0243371 51.064 47 21 1 62 106 45 91 8.76e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0462960 AT5G61430.1 52.817 142 28 2 13 116 10 150 3.49e-42 142
Msa0462960 AT3G29035.1 52.083 144 31 1 3 108 8 151 9.55e-42 140
Msa0462960 AT5G07680.1 52.083 144 30 2 11 116 9 151 1.27e-41 140
Msa0462960 AT5G07680.2 52.899 138 26 2 17 116 1 137 2.46e-40 136
Msa0462960 AT5G39610.1 52.239 134 26 1 13 108 14 147 1.54e-38 131
Msa0462960 AT3G04060.1 48.993 149 36 3 7 116 8 155 8.27e-38 130
Msa0462960 AT5G53950.1 51.799 139 26 3 19 117 17 154 2.62e-37 130
Msa0462960 AT3G18400.1 49.219 128 27 1 19 108 5 132 2.64e-37 128
Msa0462960 AT5G18270.2 50.355 141 30 3 15 116 17 156 6.48e-37 128
Msa0462960 AT5G18270.1 50.355 141 30 3 15 116 17 156 6.91e-37 128
Msa0462960 AT2G24430.2 44.521 146 41 2 5 110 2 147 1.52e-33 119
Msa0462960 AT2G24430.1 44.521 146 41 2 5 110 2 147 1.52e-33 119
Msa0462960 AT3G15170.1 45.946 148 38 4 11 118 13 158 3.83e-33 117
Msa0462960 AT1G76420.1 45.070 142 37 3 13 114 17 157 7.12e-31 112
Msa0462960 AT3G12977.1 36.641 131 45 1 19 111 18 148 1.05e-27 103
Msa0462960 AT1G56010.2 34.459 148 58 2 1 109 1 148 5.30e-27 102
Msa0462960 AT2G46770.1 36.054 147 53 1 14 119 11 157 1.20e-26 101
Msa0462960 AT3G10500.1 42.520 127 32 2 20 106 11 136 3.88e-26 101
Msa0462960 AT3G17730.1 39.695 131 39 1 17 107 4 134 4.54e-26 98.2
Msa0462960 AT5G04410.1 42.424 132 35 2 20 111 11 141 6.45e-26 101
Msa0462960 AT2G27300.1 40.310 129 37 2 19 108 15 142 1.07e-25 98.6
Msa0462960 AT4G36160.1 36.496 137 46 1 14 109 5 141 1.11e-25 99.4
Msa0462960 AT4G36160.3 36.496 137 46 1 14 109 5 141 1.11e-25 99.4
Msa0462960 AT4G36160.2 36.496 137 46 1 14 109 9 145 1.18e-25 99.4
Msa0462960 AT1G65910.1 38.168 131 41 1 17 107 4 134 1.32e-25 100
Msa0462960 AT1G12260.2 38.931 131 39 1 20 109 8 138 1.60e-25 98.6
Msa0462960 AT4G10350.1 40.141 142 44 1 20 120 10 151 2.22e-25 98.2
Msa0462960 AT1G12260.1 39.535 129 37 1 20 107 8 136 2.47e-25 98.6
Msa0462960 AT3G03200.1 39.310 145 45 2 17 121 4 145 6.17e-25 98.2
Msa0462960 AT1G01720.1 38.462 130 43 2 14 106 2 131 7.85e-25 95.5
Msa0462960 AT2G18060.3 36.029 136 46 1 15 109 5 140 7.99e-25 97.1
Msa0462960 AT2G18060.1 36.029 136 46 1 15 109 5 140 7.99e-25 97.1
Msa0462960 AT1G33280.1 41.085 129 35 1 20 107 9 137 1.06e-24 95.5
Msa0462960 AT3G10490.1 43.307 127 31 2 20 106 29 154 1.94e-24 93.6
Msa0462960 AT1G33060.1 42.520 127 33 1 19 105 24 150 2.15e-24 97.1
Msa0462960 AT1G33060.2 42.520 127 33 1 19 105 24 150 2.16e-24 97.1
Msa0462960 AT2G33480.1 40.000 125 40 1 17 106 13 137 2.34e-24 94.0
Msa0462960 AT2G33480.2 40.000 125 40 1 17 106 13 137 2.48e-24 94.0
Msa0462960 AT4G27410.2 38.760 129 41 1 16 106 11 139 2.71e-24 94.4
Msa0462960 AT4G35580.1 41.732 127 34 1 19 105 9 135 2.98e-24 96.3
Msa0462960 AT3G10480.2 43.307 127 31 2 20 106 29 154 4.37e-24 95.5
Msa0462960 AT3G10480.1 43.307 127 31 2 20 106 29 154 4.44e-24 95.5
Msa0462960 AT3G15500.1 39.535 129 40 1 16 106 11 139 4.96e-24 94.0
Msa0462960 AT1G71930.2 33.333 138 51 1 16 112 6 143 6.71e-24 94.0
Msa0462960 AT1G71930.1 33.333 138 51 1 16 112 6 143 6.71e-24 94.0
Msa0462960 AT4G35580.3 41.732 127 34 1 19 105 9 135 6.87e-24 95.5
Msa0462960 AT4G35580.2 41.732 127 34 1 19 105 9 135 7.23e-24 95.5
Msa0462960 AT3G61910.1 35.252 139 49 1 14 111 6 144 7.31e-24 94.0
Msa0462960 AT5G13180.1 36.364 132 47 2 12 106 7 138 8.15e-24 92.4
Msa0462960 AT5G17260.1 42.069 145 41 4 17 121 4 145 1.26e-23 94.4
Msa0462960 AT5G62380.2 36.364 132 43 1 17 107 5 136 1.32e-23 93.2
Msa0462960 AT5G62380.1 36.364 132 43 1 17 107 5 136 1.32e-23 93.2
Msa0462960 AT1G52890.1 39.535 129 40 1 16 106 11 139 1.42e-23 92.8
Msa0462960 AT1G61110.1 36.735 147 54 2 5 112 2 148 1.60e-23 92.8
Msa0462960 AT1G32770.1 36.429 140 48 1 14 112 11 150 2.02e-23 92.8
Msa0462960 AT1G26870.1 39.310 145 45 4 1 105 1 142 5.94e-23 92.4
Msa0462960 AT5G08790.1 39.535 129 39 2 16 106 4 131 8.52e-23 90.1
Msa0462960 AT5G66300.1 34.586 133 46 1 13 104 6 138 1.18e-22 90.1
Msa0462960 AT5G61430.2 72.727 55 14 1 62 116 34 87 1.33e-22 89.4
Msa0462960 AT3G10490.2 43.307 127 31 2 20 106 29 154 1.73e-22 91.3
Msa0462960 AT1G62700.1 37.778 135 43 1 14 107 2 136 1.75e-22 90.9
Msa0462960 AT1G62700.2 37.778 135 43 1 14 107 2 136 1.75e-22 90.9
Msa0462960 AT1G77450.1 37.500 128 41 2 17 106 8 134 3.30e-22 88.2
Msa0462960 AT3G04070.2 36.913 149 50 3 13 118 4 151 3.88e-22 89.4
Msa0462960 AT1G54330.1 40.000 130 38 1 17 106 4 133 5.07e-22 88.6
Msa0462960 AT5G22290.1 35.821 134 46 2 19 113 22 154 5.23e-22 89.0
Msa0462960 AT3G04070.1 36.913 149 50 3 13 118 4 151 5.46e-22 89.0
Msa0462960 AT3G49530.1 35.294 153 54 3 4 115 2 150 8.10e-22 89.4
Msa0462960 AT3G49530.2 35.294 153 54 3 4 115 2 150 1.63e-21 88.2
Msa0462960 AT1G79580.3 37.313 134 42 2 16 107 14 147 2.51e-21 87.4
Msa0462960 AT1G79580.5 37.313 134 42 2 16 107 14 147 2.51e-21 87.4
Msa0462960 AT1G79580.4 37.313 134 42 2 16 107 14 147 2.51e-21 87.4
Msa0462960 AT1G79580.2 37.313 134 42 2 16 107 14 147 2.51e-21 87.4
Msa0462960 AT1G79580.1 37.313 134 42 2 16 107 14 147 2.51e-21 87.4
Msa0462960 AT5G63790.1 38.060 134 43 3 11 106 43 174 3.81e-21 86.3
Msa0462960 AT5G63790.2 38.060 134 43 3 11 106 53 184 4.32e-21 86.3
Msa0462960 AT3G44290.2 37.121 132 43 2 19 111 15 145 9.37e-21 84.0
Msa0462960 AT2G43000.1 31.126 151 65 2 1 113 1 150 9.76e-21 84.7
Msa0462960 AT5G46590.1 33.566 143 54 2 19 120 6 148 1.47e-20 84.3
Msa0462960 AT5G46590.2 34.559 136 48 2 19 113 6 141 1.87e-20 84.0
Msa0462960 AT4G27410.3 34.247 146 41 2 16 106 11 156 1.94e-20 84.3
Msa0462960 AT3G15510.1 37.097 124 38 2 23 106 21 144 3.20e-20 84.3
Msa0462960 AT1G69490.1 37.302 126 41 1 19 106 9 134 3.29e-20 83.2
Msa0462960 AT3G44290.1 37.121 132 43 2 19 111 15 145 3.33e-20 84.0
Msa0462960 AT5G24590.2 37.500 128 39 2 19 105 13 140 4.41e-20 84.3
Msa0462960 AT5G64060.1 33.577 137 50 2 20 116 8 143 9.86e-20 82.8
Msa0462960 AT4G17980.1 36.154 130 41 2 19 106 6 135 1.92e-19 80.9
Msa0462960 AT4G17980.2 36.154 130 41 2 19 106 6 135 3.16e-19 80.9
Msa0462960 AT4G28530.1 32.192 146 43 3 18 107 9 154 3.23e-19 81.6
Msa0462960 AT5G09330.5 34.307 137 49 2 20 116 8 143 3.88e-19 82.0
Msa0462960 AT5G09330.2 34.307 137 49 2 20 116 8 143 3.88e-19 82.0
Msa0462960 AT5G09330.4 34.307 137 49 2 20 116 8 143 3.88e-19 82.0
Msa0462960 AT5G09330.3 34.307 137 49 2 20 116 8 143 3.88e-19 82.0
Msa0462960 AT5G09330.1 34.307 137 49 2 20 116 8 143 3.88e-19 82.0
Msa0462960 AT1G34180.3 36.364 132 43 2 16 107 14 144 1.28e-18 80.5
Msa0462960 AT1G34190.1 36.029 136 46 2 16 111 14 148 2.05e-18 80.1
Msa0462960 AT1G32510.1 33.108 148 52 2 20 120 7 154 4.06e-18 77.8
Msa0462960 AT1G34180.1 36.029 136 46 2 16 111 14 148 7.92e-18 78.2
Msa0462960 AT2G17040.1 34.328 134 46 3 17 109 5 137 3.82e-17 75.1
Msa0462960 AT3G12977.2 58.000 50 21 0 62 111 46 95 2.28e-16 72.4
Msa0462960 AT1G56010.1 60.417 48 19 0 62 109 34 81 2.74e-16 72.4
Msa0462960 AT1G34180.2 33.108 148 46 2 16 111 14 160 3.18e-16 73.6
Msa0462960 AT1G32870.1 38.400 125 38 2 20 106 12 135 5.95e-16 72.8
Msa0462960 AT1G32870.2 38.400 125 38 2 20 106 12 135 6.06e-16 72.8
Msa0462960 AT1G32870.3 38.400 125 38 2 20 106 48 171 6.37e-16 72.8
Msa0462960 AT2G18060.2 58.333 48 20 0 62 109 5 52 7.18e-16 71.6
Msa0462960 AT1G34180.4 33.108 148 46 2 16 111 14 160 7.31e-16 72.4
Msa0462960 AT1G52880.1 34.884 129 42 3 23 109 21 149 7.57e-16 72.0
Msa0462960 AT3G10490.4 66.667 45 15 0 62 106 10 54 4.85e-15 70.1
Msa0462960 AT3G10490.3 66.667 45 15 0 62 106 10 54 4.85e-15 70.1
Msa0462960 AT3G10480.3 66.667 45 15 0 62 106 143 187 8.18e-15 69.7
Msa0462960 AT5G22290.2 50.000 52 26 0 62 113 4 55 1.64e-14 67.4
Msa0462960 AT4G10350.2 54.237 59 27 0 62 120 46 104 3.89e-14 67.0
Msa0462960 AT2G33480.3 60.000 45 18 0 62 106 36 80 1.76e-13 64.3
Msa0462960 AT3G56560.1 30.172 116 61 3 19 117 115 227 4.79e-11 58.2

Find 25 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAGGGAAATGCATTAAT+TGG 0.121823 3_3:-62215717 Msa0462960:CDS
AAATGAAAAGGTCGAATTTC+CGG 0.216715 3_3:-62216899 Msa0462960:CDS
AAATCATTACCTTGTCAAAA+AGG 0.228485 3_3:-62216839 Msa0462960:CDS
AATGAAAAGGTCGAATTTCC+GGG 0.351715 3_3:-62216898 Msa0462960:CDS
TCAAGGGAAATGCATTAATT+GGG 0.420828 3_3:-62215716 Msa0462960:CDS
CGAGTGGTGAAAAGACCAAT+TGG 0.426528 3_3:-62215656 Msa0462960:CDS
GTATTCATGCATGACCCAAT+TGG 0.496635 3_3:+62215641 None:intergenic
TTACCATTCCATGTGTGGAT+AGG 0.526862 3_3:+62215586 None:intergenic
ATGATTTATAAGCTCTTCAT+CGG 0.528076 3_3:+62216855 None:intergenic
TTTATAAGCTCTTCATCGGT+CGG 0.530344 3_3:+62216859 None:intergenic
AATTGGGATGAAGAAAACAT+TGG 0.537739 3_3:-62215700 Msa0462960:CDS
GAGTGGTGAAAAGACCAATT+GGG 0.539850 3_3:-62215655 Msa0462960:CDS
CAACCTATCCACACATGGAA+TGG 0.547037 3_3:-62215589 None:intergenic
AATTTACCTGGCAAATCCCA+TGG 0.560572 3_3:+62216757 None:intergenic
TGTGAACCATGGGATTTGCC+AGG 0.562377 3_3:-62216763 Msa0462960:intron
CAATATTACCATTCCATGTG+TGG 0.569497 3_3:+62215581 None:intergenic
GGTCATGCATGAATACCGAT+TGG 0.581760 3_3:-62215634 Msa0462960:CDS
AGAGTGTGTTACCCTCCAAT+CGG 0.588761 3_3:+62215619 None:intergenic
CATGCATGAATACCGATTGG+AGG 0.592763 3_3:-62215631 Msa0462960:CDS
ATGCATGAATACCGATTGGA+GGG 0.621001 3_3:-62215630 Msa0462960:CDS
TATGAATAAGTGTGAACCAT+GGG 0.641317 3_3:-62216773 Msa0462960:CDS
GAGCACAACCTATCCACACA+TGG 0.663138 3_3:-62215594 Msa0462960:CDS
ATATGAATAAGTGTGAACCA+TGG 0.681966 3_3:-62216774 Msa0462960:CDS
GGATAGAATCGAAAACAACC+CGG 0.686902 3_3:+62216880 None:intergenic
TACATAGGAAGAGCTCCGAG+TGG 0.745847 3_3:-62215672 Msa0462960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATATTACTAATTTTTTTTT+TGG + chr3_3:62215852-62215871 None:intergenic 10.0%
!! CAAAAAAAAAATTAGTAATA+TGG - chr3_3:62215850-62215869 Msa0462960:intron 10.0%
!!! CATATTACTAATTTTTTTTT+TGG + chr3_3:62215852-62215871 None:intergenic 10.0%
!! CAAAAAAAAAATTAGTAATA+TGG - chr3_3:62215850-62215869 Msa0462960:intron 10.0%
!! AAAAAAACATTAAATTTACC+TGG + chr3_3:62215785-62215804 None:intergenic 15.0%
!! AAAAAGAAAGAAAAACAATT+TGG - chr3_3:62216280-62216299 Msa0462960:intron 15.0%
!! AAAAAAACATTAAATTTACC+TGG + chr3_3:62215785-62215804 None:intergenic 15.0%
!! AAAAAGAAAGAAAAACAATT+TGG - chr3_3:62216280-62216299 Msa0462960:intron 15.0%
!!! TTCATTTTCCATTTTTTGAT+TGG + chr3_3:62215615-62215634 None:intergenic 20.0%
!! TCAAAAAATGGAAAATGAAA+AGG - chr3_3:62215616-62215635 Msa0462960:CDS 20.0%
!! ATGAAAAAGAAGAAGAAAAA+TGG - chr3_3:62216358-62216377 Msa0462960:intron 20.0%
!!! AATTATGCTTTCCTATTTTT+TGG - chr3_3:62216647-62216666 Msa0462960:intron 20.0%
!!! TTCATTTTCCATTTTTTGAT+TGG + chr3_3:62215615-62215634 None:intergenic 20.0%
!! TCAAAAAATGGAAAATGAAA+AGG - chr3_3:62215616-62215635 Msa0462960:CDS 20.0%
!! ATGAAAAAGAAGAAGAAAAA+TGG - chr3_3:62216358-62216377 Msa0462960:intron 20.0%
!!! AATTATGCTTTCCTATTTTT+TGG - chr3_3:62216647-62216666 Msa0462960:intron 20.0%
! GAATGTTTCCAATCAAAAAA+TGG - chr3_3:62215604-62215623 Msa0462960:CDS 25.0%
! ATGATTTATAAGCTCTTCAT+CGG + chr3_3:62215675-62215694 None:intergenic 25.0%
! AAATCATTACCTTGTCAAAA+AGG - chr3_3:62215688-62215707 Msa0462960:CDS 25.0%
! CACATAAATTAAGTCTTCTT+TGG + chr3_3:62216239-62216258 None:intergenic 25.0%
! CAAAGAAGACTTAATTTATG+TGG - chr3_3:62216237-62216256 Msa0462960:intron 25.0%
! GAAAGAAAAACAATTTGGAA+TGG - chr3_3:62216285-62216304 Msa0462960:intron 25.0%
!! TGATAACGACAATATTTCTA+AGG + chr3_3:62216430-62216449 None:intergenic 25.0%
!!! TTTTAAAGTTATTACGAGTG+TGG - chr3_3:62216569-62216588 Msa0462960:intron 25.0%
! AATGTATGTACTATGTCTTT+CGG - chr3_3:62216611-62216630 Msa0462960:intron 25.0%
! CTCTGAATAAACCAAAAAAT+AGG + chr3_3:62216661-62216680 None:intergenic 25.0%
!!! TTTTTTGGTTTATTCAGAGA+TGG - chr3_3:62216662-62216681 Msa0462960:intron 25.0%
!! AAAGACAAAGAGATTTTCAA+GGG - chr3_3:62216795-62216814 Msa0462960:CDS 25.0%
!!! AAAACATTGGTTTTCTACAT+AGG - chr3_3:62216840-62216859 Msa0462960:CDS 25.0%
! GAATGTTTCCAATCAAAAAA+TGG - chr3_3:62215604-62215623 Msa0462960:CDS 25.0%
! ATGATTTATAAGCTCTTCAT+CGG + chr3_3:62215675-62215694 None:intergenic 25.0%
! AAATCATTACCTTGTCAAAA+AGG - chr3_3:62215688-62215707 Msa0462960:CDS 25.0%
! CACATAAATTAAGTCTTCTT+TGG + chr3_3:62216239-62216258 None:intergenic 25.0%
! CAAAGAAGACTTAATTTATG+TGG - chr3_3:62216237-62216256 Msa0462960:intron 25.0%
! GAAAGAAAAACAATTTGGAA+TGG - chr3_3:62216285-62216304 Msa0462960:intron 25.0%
!! TGATAACGACAATATTTCTA+AGG + chr3_3:62216430-62216449 None:intergenic 25.0%
!!! TTTTAAAGTTATTACGAGTG+TGG - chr3_3:62216569-62216588 Msa0462960:intron 25.0%
! AATGTATGTACTATGTCTTT+CGG - chr3_3:62216611-62216630 Msa0462960:intron 25.0%
! CTCTGAATAAACCAAAAAAT+AGG + chr3_3:62216661-62216680 None:intergenic 25.0%
!!! TTTTTTGGTTTATTCAGAGA+TGG - chr3_3:62216662-62216681 Msa0462960:intron 25.0%
!! AAAGACAAAGAGATTTTCAA+GGG - chr3_3:62216795-62216814 Msa0462960:CDS 25.0%
!!! AAAACATTGGTTTTCTACAT+AGG - chr3_3:62216840-62216859 Msa0462960:CDS 25.0%
AAATGAAAAGGTCGAATTTC+CGG - chr3_3:62215628-62215647 Msa0462960:CDS 30.0%
ATATGAATAAGTGTGAACCA+TGG - chr3_3:62215753-62215772 Msa0462960:intron 30.0%
TATGAATAAGTGTGAACCAT+GGG - chr3_3:62215754-62215773 Msa0462960:intron 30.0%
! TTTTCGCAAATTCATCTGTT+TGG + chr3_3:62215974-62215993 None:intergenic 30.0%
ACAGATGAATTTGCGAAAAA+AGG - chr3_3:62215975-62215994 Msa0462960:intron 30.0%
ACACATGAGTATAGTCATAT+TGG + chr3_3:62216020-62216039 None:intergenic 30.0%
CATCAATGTTACAGATATGT+AGG + chr3_3:62216057-62216076 None:intergenic 30.0%
! ACAATTTGGAATGGTGATTA+CGG - chr3_3:62216294-62216313 Msa0462960:intron 30.0%
! TACCAATGAAGGATGTTTTA+GGG + chr3_3:62216331-62216350 None:intergenic 30.0%
TCGTTATCATCTTACTTTGA+TGG - chr3_3:62216441-62216460 Msa0462960:intron 30.0%
CGTTATCATCTTACTTTGAT+GGG - chr3_3:62216442-62216461 Msa0462960:intron 30.0%
TTATTCAGAGATGGCTAAAA+TGG - chr3_3:62216671-62216690 Msa0462960:intron 30.0%
TATTCAGAGATGGCTAAAAT+GGG - chr3_3:62216672-62216691 Msa0462960:intron 30.0%
!!! AATGGTATTTTTTCTGTGTG+AGG - chr3_3:62216703-62216722 Msa0462960:intron 30.0%
!!! ATGGTATTTTTTCTGTGTGA+GGG - chr3_3:62216704-62216723 Msa0462960:intron 30.0%
! CAAAGACAAAGAGATTTTCA+AGG - chr3_3:62216794-62216813 Msa0462960:CDS 30.0%
TTCAAGGGAAATGCATTAAT+TGG - chr3_3:62216810-62216829 Msa0462960:CDS 30.0%
TCAAGGGAAATGCATTAATT+GGG - chr3_3:62216811-62216830 Msa0462960:CDS 30.0%
AATTGGGATGAAGAAAACAT+TGG - chr3_3:62216827-62216846 Msa0462960:CDS 30.0%
AAATGAAAAGGTCGAATTTC+CGG - chr3_3:62215628-62215647 Msa0462960:CDS 30.0%
ATATGAATAAGTGTGAACCA+TGG - chr3_3:62215753-62215772 Msa0462960:intron 30.0%
TATGAATAAGTGTGAACCAT+GGG - chr3_3:62215754-62215773 Msa0462960:intron 30.0%
! TTTTCGCAAATTCATCTGTT+TGG + chr3_3:62215974-62215993 None:intergenic 30.0%
ACAGATGAATTTGCGAAAAA+AGG - chr3_3:62215975-62215994 Msa0462960:intron 30.0%
ACACATGAGTATAGTCATAT+TGG + chr3_3:62216020-62216039 None:intergenic 30.0%
CATCAATGTTACAGATATGT+AGG + chr3_3:62216057-62216076 None:intergenic 30.0%
! ACAATTTGGAATGGTGATTA+CGG - chr3_3:62216294-62216313 Msa0462960:intron 30.0%
! TACCAATGAAGGATGTTTTA+GGG + chr3_3:62216331-62216350 None:intergenic 30.0%
TCGTTATCATCTTACTTTGA+TGG - chr3_3:62216441-62216460 Msa0462960:intron 30.0%
CGTTATCATCTTACTTTGAT+GGG - chr3_3:62216442-62216461 Msa0462960:intron 30.0%
TTATTCAGAGATGGCTAAAA+TGG - chr3_3:62216671-62216690 Msa0462960:intron 30.0%
TATTCAGAGATGGCTAAAAT+GGG - chr3_3:62216672-62216691 Msa0462960:intron 30.0%
!!! AATGGTATTTTTTCTGTGTG+AGG - chr3_3:62216703-62216722 Msa0462960:intron 30.0%
!!! ATGGTATTTTTTCTGTGTGA+GGG - chr3_3:62216704-62216723 Msa0462960:intron 30.0%
! CAAAGACAAAGAGATTTTCA+AGG - chr3_3:62216794-62216813 Msa0462960:CDS 30.0%
TTCAAGGGAAATGCATTAAT+TGG - chr3_3:62216810-62216829 Msa0462960:CDS 30.0%
TCAAGGGAAATGCATTAATT+GGG - chr3_3:62216811-62216830 Msa0462960:CDS 30.0%
AATTGGGATGAAGAAAACAT+TGG - chr3_3:62216827-62216846 Msa0462960:CDS 30.0%
AATGAAAAGGTCGAATTTCC+GGG - chr3_3:62215629-62215648 Msa0462960:CDS 35.0%
TTTATAAGCTCTTCATCGGT+CGG + chr3_3:62215671-62215690 None:intergenic 35.0%
! TGTCATCAACCTTTTTGACA+AGG + chr3_3:62215700-62215719 None:intergenic 35.0%
CCAGATCTAATCAATGACTT+TGG + chr3_3:62216083-62216102 None:intergenic 35.0%
! CCAAAGTCATTGATTAGATC+TGG - chr3_3:62216080-62216099 Msa0462960:intron 35.0%
ATGCAGTGTTTATTTGACTC+AGG + chr3_3:62216130-62216149 None:intergenic 35.0%
CAAACAATGTCCTACTAAGA+CGG - chr3_3:62216195-62216214 Msa0462960:intron 35.0%
TATGATTTCACCGTCTTAGT+AGG + chr3_3:62216208-62216227 None:intergenic 35.0%
! CAATTTGGAATGGTGATTAC+GGG - chr3_3:62216295-62216314 Msa0462960:intron 35.0%
AACCCTAAAACATCCTTCAT+TGG - chr3_3:62216326-62216345 Msa0462960:intron 35.0%
! CTACCAATGAAGGATGTTTT+AGG + chr3_3:62216332-62216351 None:intergenic 35.0%
AAGAAAAATGGTGACCACTA+GGG - chr3_3:62216370-62216389 Msa0462960:intron 35.0%
CCTTACACATTATGATGTGT+CGG + chr3_3:62216498-62216517 None:intergenic 35.0%
AATGAAAAGGTCGAATTTCC+GGG - chr3_3:62215629-62215648 Msa0462960:CDS 35.0%
TTTATAAGCTCTTCATCGGT+CGG + chr3_3:62215671-62215690 None:intergenic 35.0%
! TGTCATCAACCTTTTTGACA+AGG + chr3_3:62215700-62215719 None:intergenic 35.0%
CCAGATCTAATCAATGACTT+TGG + chr3_3:62216083-62216102 None:intergenic 35.0%
! CCAAAGTCATTGATTAGATC+TGG - chr3_3:62216080-62216099 Msa0462960:intron 35.0%
ATGCAGTGTTTATTTGACTC+AGG + chr3_3:62216130-62216149 None:intergenic 35.0%
CAAACAATGTCCTACTAAGA+CGG - chr3_3:62216195-62216214 Msa0462960:intron 35.0%
TATGATTTCACCGTCTTAGT+AGG + chr3_3:62216208-62216227 None:intergenic 35.0%
! CAATTTGGAATGGTGATTAC+GGG - chr3_3:62216295-62216314 Msa0462960:intron 35.0%
AACCCTAAAACATCCTTCAT+TGG - chr3_3:62216326-62216345 Msa0462960:intron 35.0%
! CTACCAATGAAGGATGTTTT+AGG + chr3_3:62216332-62216351 None:intergenic 35.0%
AAGAAAAATGGTGACCACTA+GGG - chr3_3:62216370-62216389 Msa0462960:intron 35.0%
CCTTACACATTATGATGTGT+CGG + chr3_3:62216498-62216517 None:intergenic 35.0%
GGATAGAATCGAAAACAACC+CGG + chr3_3:62215650-62215669 None:intergenic 40.0%
AATTTACCTGGCAAATCCCA+TGG + chr3_3:62215773-62215792 None:intergenic 40.0%
ACATCCTTCATTGGTAGATC+TGG - chr3_3:62216335-62216354 Msa0462960:intron 40.0%
TCATCCAGATCTACCAATGA+AGG + chr3_3:62216342-62216361 None:intergenic 40.0%
GAAGAAAAATGGTGACCACT+AGG - chr3_3:62216369-62216388 Msa0462960:intron 40.0%
CCGACACATCATAATGTGTA+AGG - chr3_3:62216495-62216514 Msa0462960:intron 40.0%
! CACATCTGATAGTGTCATGT+TGG + chr3_3:62216529-62216548 None:intergenic 40.0%
CAACATGACACTATCAGATG+TGG - chr3_3:62216527-62216546 Msa0462960:intron 40.0%
CTAAAATGGGAGAAGCAGAA+TGG - chr3_3:62216685-62216704 Msa0462960:intron 40.0%
GAAAATACCCAACTGGTCAA+AGG - chr3_3:62216730-62216749 Msa0462960:intron 40.0%
TATTGGTCCTTTGACCAGTT+GGG + chr3_3:62216740-62216759 None:intergenic 40.0%
ACCAATAGAGCTACTAATGC+TGG - chr3_3:62216753-62216772 Msa0462960:intron 40.0%
! TCCAGCATTAGTAGCTCTAT+TGG + chr3_3:62216757-62216776 None:intergenic 40.0%
GAGCTACTAATGCTGGATAT+TGG - chr3_3:62216760-62216779 Msa0462960:intron 40.0%
!!! CAATTGGTCTTTTCACCACT+CGG + chr3_3:62216873-62216892 None:intergenic 40.0%
GAGTGGTGAAAAGACCAATT+GGG - chr3_3:62216872-62216891 Msa0462960:CDS 40.0%
GTATTCATGCATGACCCAAT+TGG + chr3_3:62216889-62216908 None:intergenic 40.0%
! ATGCATGAATACCGATTGGA+GGG - chr3_3:62216897-62216916 Msa0462960:CDS 40.0%
GGATAGAATCGAAAACAACC+CGG + chr3_3:62215650-62215669 None:intergenic 40.0%
AATTTACCTGGCAAATCCCA+TGG + chr3_3:62215773-62215792 None:intergenic 40.0%
ACATCCTTCATTGGTAGATC+TGG - chr3_3:62216335-62216354 Msa0462960:intron 40.0%
TCATCCAGATCTACCAATGA+AGG + chr3_3:62216342-62216361 None:intergenic 40.0%
GAAGAAAAATGGTGACCACT+AGG - chr3_3:62216369-62216388 Msa0462960:intron 40.0%
CCGACACATCATAATGTGTA+AGG - chr3_3:62216495-62216514 Msa0462960:intron 40.0%
! CACATCTGATAGTGTCATGT+TGG + chr3_3:62216529-62216548 None:intergenic 40.0%
CAACATGACACTATCAGATG+TGG - chr3_3:62216527-62216546 Msa0462960:intron 40.0%
CTAAAATGGGAGAAGCAGAA+TGG - chr3_3:62216685-62216704 Msa0462960:intron 40.0%
GAAAATACCCAACTGGTCAA+AGG - chr3_3:62216730-62216749 Msa0462960:intron 40.0%
TATTGGTCCTTTGACCAGTT+GGG + chr3_3:62216740-62216759 None:intergenic 40.0%
ACCAATAGAGCTACTAATGC+TGG - chr3_3:62216753-62216772 Msa0462960:intron 40.0%
! TCCAGCATTAGTAGCTCTAT+TGG + chr3_3:62216757-62216776 None:intergenic 40.0%
GAGCTACTAATGCTGGATAT+TGG - chr3_3:62216760-62216779 Msa0462960:intron 40.0%
!!! CAATTGGTCTTTTCACCACT+CGG + chr3_3:62216873-62216892 None:intergenic 40.0%
GAGTGGTGAAAAGACCAATT+GGG - chr3_3:62216872-62216891 Msa0462960:CDS 40.0%
GTATTCATGCATGACCCAAT+TGG + chr3_3:62216889-62216908 None:intergenic 40.0%
! ATGCATGAATACCGATTGGA+GGG - chr3_3:62216897-62216916 Msa0462960:CDS 40.0%
AAATGGTGACCACTAGGGTT+TGG - chr3_3:62216375-62216394 Msa0462960:intron 45.0%
AGGGAGAGAAAATACCCAAC+TGG - chr3_3:62216723-62216742 Msa0462960:intron 45.0%
CTATTGGTCCTTTGACCAGT+TGG + chr3_3:62216741-62216760 None:intergenic 45.0%
GCTGGATATTGGAAAGCAAC+AGG - chr3_3:62216771-62216790 Msa0462960:CDS 45.0%
CGAGTGGTGAAAAGACCAAT+TGG - chr3_3:62216871-62216890 Msa0462960:CDS 45.0%
GGTCATGCATGAATACCGAT+TGG - chr3_3:62216893-62216912 Msa0462960:CDS 45.0%
! CATGCATGAATACCGATTGG+AGG - chr3_3:62216896-62216915 Msa0462960:CDS 45.0%
AGAGTGTGTTACCCTCCAAT+CGG + chr3_3:62216911-62216930 None:intergenic 45.0%
AAATGGTGACCACTAGGGTT+TGG - chr3_3:62216375-62216394 Msa0462960:intron 45.0%
AGGGAGAGAAAATACCCAAC+TGG - chr3_3:62216723-62216742 Msa0462960:intron 45.0%
CTATTGGTCCTTTGACCAGT+TGG + chr3_3:62216741-62216760 None:intergenic 45.0%
GCTGGATATTGGAAAGCAAC+AGG - chr3_3:62216771-62216790 Msa0462960:CDS 45.0%
CGAGTGGTGAAAAGACCAAT+TGG - chr3_3:62216871-62216890 Msa0462960:CDS 45.0%
GGTCATGCATGAATACCGAT+TGG - chr3_3:62216893-62216912 Msa0462960:CDS 45.0%
! CATGCATGAATACCGATTGG+AGG - chr3_3:62216896-62216915 Msa0462960:CDS 45.0%
AGAGTGTGTTACCCTCCAAT+CGG + chr3_3:62216911-62216930 None:intergenic 45.0%
TGTGAACCATGGGATTTGCC+AGG - chr3_3:62215764-62215783 Msa0462960:intron 50.0%
TTCTCCTTGCCAAACCCTAG+TGG + chr3_3:62216387-62216406 None:intergenic 50.0%
TTGGCAAGGAGAAAAGCGAG+TGG - chr3_3:62216394-62216413 Msa0462960:intron 50.0%
TACATAGGAAGAGCTCCGAG+TGG - chr3_3:62216855-62216874 Msa0462960:CDS 50.0%
GAGCACAACCTATCCACACA+TGG - chr3_3:62216933-62216952 Msa0462960:CDS 50.0%
TGTGAACCATGGGATTTGCC+AGG - chr3_3:62215764-62215783 Msa0462960:intron 50.0%
TTCTCCTTGCCAAACCCTAG+TGG + chr3_3:62216387-62216406 None:intergenic 50.0%
TTGGCAAGGAGAAAAGCGAG+TGG - chr3_3:62216394-62216413 Msa0462960:intron 50.0%
TACATAGGAAGAGCTCCGAG+TGG - chr3_3:62216855-62216874 Msa0462960:CDS 50.0%
GAGCACAACCTATCCACACA+TGG - chr3_3:62216933-62216952 Msa0462960:CDS 50.0%
GTGACCACTAGGGTTTGGCA+AGG - chr3_3:62216380-62216399 Msa0462960:intron 55.0%
GTGACCACTAGGGTTTGGCA+AGG - chr3_3:62216380-62216399 Msa0462960:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 62215590 62216959 62215590 ID=Msa0462960;Name=Msa0462960
chr3_3 mRNA 62215590 62216959 62215590 ID=Msa0462960-mRNA-1;Parent=Msa0462960;Name=Msa0462960-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|0|0|1|1|0.5|2|0|121
chr3_3 exon 62216764 62216959 62216764 ID=Msa0462960-mRNA-1:exon:13294;Parent=Msa0462960-mRNA-1
chr3_3 exon 62215590 62215756 62215590 ID=Msa0462960-mRNA-1:exon:13293;Parent=Msa0462960-mRNA-1
chr3_3 CDS 62216764 62216959 62216764 ID=Msa0462960-mRNA-1:cds;Parent=Msa0462960-mRNA-1
chr3_3 CDS 62215590 62215756 62215590 ID=Msa0462960-mRNA-1:cds;Parent=Msa0462960-mRNA-1
Gene Sequence

>Msa0462960

ATGGGATCAAATGAGAATGTTTCCAATCAAAAAATGGAAAATGAAAAGGTCGAATTTCCGGGTTGTTTTCGATTCTATCCGACCGATGAAGAGCTTATAAATCATTACCTTGTCAAAAAGGTTGATGACAATAGCTTCTCTGCTATAGCTATTGCTGAAGTTGATATGAATAAGTGTGAACCATGGGATTTGCCAGGCAAAGACAAAGAGATTTTCAAGGGAAATGCATTAATTGGGATGAAGAAAACATTGGTTTTCTACATAGGAAGAGCTCCGAGTGGTGAAAAGACCAATTGGGTCATGCATGAATACCGATTGGAGGGTAACACACTCTCTGAGCACAACCTATCCACACATGGAATG
Protein sequence

>Msa0462960

MGSNENVSNQKMENEKVEFPGCFRFYPTDEELINHYLVKKVDDNSFSAIAIAEVDMNKCEPWDLPGKDKEIFKGNALIGMKKTLVFYIGRAPSGEKTNWVMHEYRLEGNTLSEHNLSTHGM