AlfalfaGEDB | Msa0464660

M. sativa cultivar zhongmu-4 / Msa0464660

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0464660	XP_024630916.2	96.953	722	22	0	1	722	1	722	0.0	1462

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0464660	sp\|Q9SZL8\|FRS5_ARATH	36.991	565	344	7	34	589	73	634	4.90e-116	384

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0464660	A0A3Q7Y261	80.249	724	138	2	1	722	1	721	0.0	1196

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TFs/TRs:

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Gene ID	Type	Classification
Msa0464660	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0464660	MtrunA17_Chr3g0082121	71.758	694	184	3	1	690	8	693	0.0	1011
Msa0464660	MtrunA17_Chr2g0324011	46.274	577	301	6	34	604	10	583	1.89e-171	522
Msa0464660	MtrunA17_Chr1g0164591	73.016	315	77	1	112	426	1	307	2.47e-162	488
Msa0464660	MtrunA17_Chr6g0458391	41.828	569	306	8	27	590	218	766	9.63e-147	463
Msa0464660	MtrunA17_Chr6g0458391	40.602	133	77	2	33	165	90	220	5.54e-24	109
Msa0464660	MtrunA17_Chr1g0203721	38.509	644	385	6	36	670	65	706	3.10e-145	462
Msa0464660	MtrunA17_Chr5g0448761	33.193	711	444	9	34	727	60	756	9.12e-121	394
Msa0464660	MtrunA17_Chr1g0202341	34.615	572	361	6	34	597	51	617	2.69e-106	352
Msa0464660	MtrunA17_Chr8g0363841	32.689	569	360	9	35	589	18	577	1.22e-97	327
Msa0464660	MtrunA17_Chr2g0287651	31.866	659	410	14	36	680	77	710	3.39e-94	323

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0464660	AT4G38180.1	36.991	565	344	7	34	589	73	634	4.98e-117	384
Msa0464660	AT4G15090.1	29.167	696	475	8	34	723	51	734	1.26e-90	312
Msa0464660	AT4G15090.5	29.167	696	475	8	34	723	51	734	1.26e-90	312
Msa0464660	AT4G15090.3	29.167	696	475	8	34	723	51	734	1.26e-90	312
Msa0464660	AT4G15090.2	29.167	696	475	8	34	723	51	734	1.26e-90	312
Msa0464660	AT4G15090.4	29.167	696	475	8	34	723	51	734	1.26e-90	312
Msa0464660	AT3G22170.2	29.936	628	379	12	31	634	67	657	1.46e-85	298
Msa0464660	AT3G22170.1	29.936	628	379	12	31	634	67	657	1.46e-85	298
Msa0464660	AT1G76320.3	31.405	605	386	13	38	634	1	584	6.18e-85	292
Msa0464660	AT1G76320.2	31.291	604	388	12	38	634	1	584	4.14e-84	291

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Find 287 sgRNAs with CRISPR-Local

Find 541 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAAGTATTGCCAATAG+TGG	0.483375	3_3:+64739323	Msa0464660:CDS
AAAGAGTTCGCGATTCCGTT+TGG	0.365271	3_3:+64740333	Msa0464660:CDS
AAAGGGCTGTCAATGCTAAA+CGG	0.496120	3_3:+64740617	Msa0464660:CDS
AAATAAGGATTATCGACCTT+TGG	0.372421	3_3:+64740048	Msa0464660:CDS
AAATCAAAATTGGCGACTCG+TGG	0.613179	3_3:+64746068	Msa0464660:CDS
AAATGGTCACTGAGATCATG+AGG	0.608844	3_3:-64744515	None:intergenic
AAATTGGCAAGGGTAAAAGT+GGG	0.630892	3_3:+64742949	Msa0464660:CDS
AACACTACATACTAAAACGA+TGG	0.530470	3_3:+64740977	Msa0464660:CDS
AACAGATCAAGATGCAGCAA+TGG	0.573110	3_3:+64740213	Msa0464660:CDS
AACAGGATGCACAGCGCATA+TGG	0.440155	3_3:+64739562	Msa0464660:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATGAACAAAATGTTCATCA+CGG	+	chr3_3:64739299-64739318	Msa0464660:CDS	30.0%
ATGAACAAAATGTTCATCAC+GGG	+	chr3_3:64739300-64739319	Msa0464660:CDS	30.0%
GGAAATAAAAGCAGAATAGA+TGG	+	chr3_3:64739443-64739462	Msa0464660:CDS	30.0%
ATTTCACTTGATCGAAAGAT+AGG	+	chr3_3:64739587-64739606	Msa0464660:CDS	30.0%
TTTCACTTGATCGAAAGATA+GGG	+	chr3_3:64739588-64739607	Msa0464660:CDS	30.0%
TCGAAAGATAGGGAAATATA+AGG	+	chr3_3:64739598-64739617	Msa0464660:CDS	30.0%
ACAAGCATCACAAATTGTAT+TGG	+	chr3_3:64739709-64739728	Msa0464660:CDS	30.0%
CAAGCATCACAAATTGTATT+GGG	+	chr3_3:64739710-64739729	Msa0464660:CDS	30.0%
ATATATTGATGCAACTCTCT+TGG	-	chr3_3:64739755-64739774	None:intergenic	30.0%
TAAGCAAATTCTTAAGGTCT+TGG	-	chr3_3:64739820-64739839	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	64739248	64746166	64739248	ID=Msa0464660;Name=Msa0464660
chr3_3	mRNA	64739248	64746166	64739248	ID=Msa0464660-mRNA-1;Parent=Msa0464660;Name=Msa0464660-mRNA-1;_AED=0.33;_eAED=0.38;_QI=0\|0\|0\|0.22\|1\|1\|9\|0\|1297
chr3_3	exon	64739248	64741424	64739248	ID=Msa0464660-mRNA-1:exon:14276;Parent=Msa0464660-mRNA-1
chr3_3	exon	64741522	64741560	64741522	ID=Msa0464660-mRNA-1:exon:14277;Parent=Msa0464660-mRNA-1
chr3_3	exon	64741655	64741724	64741655	ID=Msa0464660-mRNA-1:exon:14278;Parent=Msa0464660-mRNA-1
chr3_3	exon	64741924	64742117	64741924	ID=Msa0464660-mRNA-1:exon:14279;Parent=Msa0464660-mRNA-1
chr3_3	exon	64742217	64742310	64742217	ID=Msa0464660-mRNA-1:exon:14280;Parent=Msa0464660-mRNA-1
chr3_3	exon	64742949	64743155	64742949	ID=Msa0464660-mRNA-1:exon:14281;Parent=Msa0464660-mRNA-1
chr3_3	exon	64744062	64744527	64744062	ID=Msa0464660-mRNA-1:exon:14282;Parent=Msa0464660-mRNA-1
chr3_3	exon	64745464	64745844	64745464	ID=Msa0464660-mRNA-1:exon:14283;Parent=Msa0464660-mRNA-1
chr3_3	exon	64745901	64746166	64745901	ID=Msa0464660-mRNA-1:exon:14284;Parent=Msa0464660-mRNA-1
chr3_3	CDS	64739248	64741424	64739248	ID=Msa0464660-mRNA-1:cds;Parent=Msa0464660-mRNA-1
chr3_3	CDS	64741522	64741560	64741522	ID=Msa0464660-mRNA-1:cds;Parent=Msa0464660-mRNA-1
chr3_3	CDS	64741655	64741724	64741655	ID=Msa0464660-mRNA-1:cds;Parent=Msa0464660-mRNA-1
chr3_3	CDS	64741924	64742117	64741924	ID=Msa0464660-mRNA-1:cds;Parent=Msa0464660-mRNA-1
chr3_3	CDS	64742217	64742310	64742217	ID=Msa0464660-mRNA-1:cds;Parent=Msa0464660-mRNA-1
chr3_3	CDS	64742949	64743155	64742949	ID=Msa0464660-mRNA-1:cds;Parent=Msa0464660-mRNA-1
chr3_3	CDS	64744062	64744527	64744062	ID=Msa0464660-mRNA-1:cds;Parent=Msa0464660-mRNA-1
chr3_3	CDS	64745464	64745844	64745464	ID=Msa0464660-mRNA-1:cds;Parent=Msa0464660-mRNA-1
chr3_3	CDS	64745901	64746166	64745901	ID=Msa0464660-mRNA-1:cds;Parent=Msa0464660-mRNA-1

Gene Sequence

>Msa0464660

ATGGATTCATCATCAATTCCAGATTGTGTCGATAAATGGGTGAACAATATCCATGAACAAAATGTTCATCACGGGAAAGAAAGTATTGCCAATAGTGGACCTCACTTGGGAATGCAGTTTAAATCTGAGGCGGTGGCATATGAATTTTATAATGATTACAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGAAATAAAAGCAGAATAGATGGAGTTTTGACTTCGAGAAGATTCACATGCTTCAAAGAGGGTGCGAAAGTTGTTGACAAAAGACGTCAACCGACGGCGGAGTCTAGAGCAGAAACGAGAACAGGATGCACAGCGCATATGGTTATTTCACTTGATCGAAAGATAGGGAAATATAAGGTTGTTGACTTTGAAGCTCAACATAACCACCTTCTGGTTCCACCTGAGTATGTTCATATGATTCGCTCCCATCGACACATATCTGAGGCACAAGCATCACAAATTGTATTGGGAGATGAATCTGGCTTAAGACCAAGAGAGTTGCATCAATATATATCCAAGCAAGCTGGTAGGGTAGAGACTGTCGGTTTTACAAGCCAAGACCTTAAGAATTTGCTTAAAACCAAAAGGAAGCAATCACTAAAGTACGGTGAAGTGGGTGCCTTGATGACATATTTCAAACAAGAAAGTGAAAATACATCATTCTTTTATGATTTTCAAATGGATGTTGATGAGCTAATAACAAATATATTTTGGGCGGATGCACAGATGATTAATGATTATGGATACTTTGGTGATATCATCACTTTTGACACCACTTACAAGACAAATAAGGATTATCGACCTTTGGGTGTATTTGTTGGACTTAATAATCACAGACAAACCGTCATTTTTGGGGCAACATTGTTATATGATGAAACAATTCCTTCTTTTCAGTGGTTATTTGAAACTTTTCTGAAAGCAATGGGTAGAGAAAAGCCTAAAACCATTCTAACAGATCAAGATGCAGCAATGGCTAAAGCTATTTCTTTAGTTATGCCAAAAACATTTCATGGATTGTGCACTTGGCATATAAGACAGAATGCTCTTAGGCATGTGAACCATCTATATCAAAAGAGTTCGCGATTCCGTTTGGATTTTGAAGCTTGCATATATCTACACGAGGAGGAAAGTGAATTTTTGAATGAATGGAATTCTTTACTTGTTGATCATAATGTTTTGAAAGATTCGTGGTTGCATTCAATTTTTAAATTGAAGGAAAAATGGGCATGGGCTTATGTTCGGAAAACATTCACGGCAGGTATGCGATCTACTCAATTAAGTGAGAGCTTTAATGCTGATTTGAAAAACCATTTGAAGTCAGATATAAATTTGGTACAATTTTTTACTCACTTTAAAAGGGCTGTCAATGCTAAACGGAACAATGAGTCAGAAGCAGAATATGATTCAAGACATAAGCTGCCTAAATTGAAAATGAAGAAGGCTCGTATGCTCTTACAAGCCGCAAATGTTTACACTCCAAAAATTTTTGAGGAATTTCAAGAGGAATATGAAGAATATCAAGATACTTGCATAAAGGAGGTGAAAGAAGGTTTGTATGTTGTCACAAATTATGATAATGCAAAGGAGAGGATGGTAATGGGAAATCCTATGGAACAAAAAGTTTCTTGTGATTGTCATAAATTTGAGACACATGGCATCTTATGTAGTCATGCTTTGAAAGTTTTAGATGTCATGAACATTAAGTTAATTCCCGAACACTACATACTAAAACGATGGACAAGAGATGCAAGGCTGGGAAGTAATCAAGGTTGGAGTGGGAGGCATGTTGAATTAGACCAAAAAGCCCATTTCATGAAGCGGTACATTGAGTTATGTCCTCAGTTGCTTAAATTGACCAATAGAGCTTCAGAATCTCATGAAACTTACATATTTTTGTCCAAAGTTTATGAAGAGTCCAGCAAAATAGTTAATGATATGTTGTCCAAAAAATATCTGGATGGAGAATCAAGTGGAATGGTTCATGTATCCATATCAATTGCGAATGATGAAGTCGACAACAACATAGACACCGTTGGCAAGGCAAAAGGCATAAAGACGAAGGATTGTCCACGTAAGAGTAAAAAACGTTATATGTCTTGGCTTGAAAAAGAACGTAAAAAGACAAAGATGCGCTCAGAAAAAAAAGAAAAAATCTCAAGAAGCAATCAAGCAACACAATTCAAGCTACACAATCAAGCCAAGAGACTTGTAAGGTTTAAGAATTTTGATGTAAAATATCACAAATCTGTTTGTTATAACAGTGACAGTGAGATTTTTGTTTTGGCGGATCCTTGTGTGGATTCTGGTGTTGTCGTTATTATTGTACAGCAGCTACTCCTTGTTCTGGTGGAGGAAGCTCTTTCTAAGCTTTTGTTATTGTTGTGTGGCTGCTGGTGTGTGTCACTGCACAGTTCTGGTGTGGTGCGCACAATTGGAGTGTTTAGGGGGCTGTTAGCGAGGCTTTGCATCATGCTTAGCCTATTTGGGTTTTTACTGTGGAGTATTTTAATCCTTGAGAAAGGTGGTTTTTTGGTTCTCCGCCTATATGCGGCACCTTTTAAATTGGCAAGGGTAAAAGTGGGTAAGAGTGAATCGACTGGTGCGGACTGGTGTGGAAGAGAAGAGCAGCAAGGTGTTTTAGTATGTGAGGAGGGCGGTGAGACGAGGGAGAGGGGCGGTGATAGGAAGAGGGGAGATGATGTGTTTAATAGACGGCGGTTCCTGGAGGCATGGCGGCGAGGGCGACGACAGAAGGGAGGCATGGCGACTGGAGGAGGGGCGTGGGACTGGAGGAGACCTTTGTGGGTGTGGGAGGAGGAGTTGGTAGAGGAGTGTAGGCAATTACTTAATGGTGTTGTTTTGCAGTATGATATTTCTGACAGGTGGCTTTGGGATTCCAACAATGATGATGTTTACACAGTGCGTGGGGCCTATCAAATTTTAACTACTATGGCCGATCCTCCTAGTGTTGGTGTCGGAGACCTTGTCTGGCATAAGCAGGTTCCTTTGAATGTTTCTATTATGGTTTGGCGTTTGTTACGGGATAGGCTTCCAACTAAGGTCAATCTCGTGAGGCGTGGGTGTCTGGATGTCGAGGCAGCTGGGTGCGGGGCAGGATGCGGTATTGCAGAAACCGCAAATCACTTGTTTCTTCATTGTTCTACTTTTGGCGCACTTTGGCAGCATATCCGTGCGTGGATTGGCGTGTCCGGTGTTGATCCTCATGATCTCATGGAGGGTGATGGTTTTTGGCTTAGGGTGTTGGTGGCTAGGTATGGTGCGGAGGATGGACGGTTGGAGGTTGGGGGTCAGAGTTGTTCTTCTTGGTGGAGGGAGATAGTGAGAATCAGGGATGGGGTAAGAGGTAGTAGTGATGGATGGTTCGCAACGTGCGTGTCGCGAAAGGTGGGGGATGGGGCGGAAACCTCTTTCTGGCGTGATTGTTGGTGTGGCGGTGTTTCTTTTCAAGATCGCTTTCGACGTCTTTATGATTTGGCTGACAACAAAGCGATCACGGTTCGGAACTTGTTTTTGTTAGGTTGGGAGGTAGGGGGAGAGGCGTTGAGGTGGCGTCGTAGCTTGGGGGTGTGGGAGGAAGAGTTGTTAGAGGAGTTGGCATCTGGACCCGATAGGGGGTATTCTGTTCGTGGTGTTTATGACATGTTGACATCGCAGGAGCATGCTCAGGTTCATCCAAGCACGAAATTAATTTGGCACAAGCAGGTTCCCTTGAAGGTCTCTATTTTTGCGTGGAGGTTGCTTCGTGATAGATTAGCAACAAAATCAAAATTGGCGACTCGTGGTGTCATCGCTACGAAGGCGCGACTGTGTGTGTTTGGGTGTGGTCACGTCGAAGACGCTCAACATTTGTTTCTATAA

Protein sequence

>Msa0464660

MDSSSIPDCVDKWVNNIHEQNVHHGKESIANSGPHLGMQFKSEAVAYEFYNDYSKRIGFGIRREYGNKSRIDGVLTSRRFTCFKEGAKVVDKRRQPTAESRAETRTGCTAHMVISLDRKIGKYKVVDFEAQHNHLLVPPEYVHMIRSHRHISEAQASQIVLGDESGLRPRELHQYISKQAGRVETVGFTSQDLKNLLKTKRKQSLKYGEVGALMTYFKQESENTSFFYDFQMDVDELITNIFWADAQMINDYGYFGDIITFDTTYKTNKDYRPLGVFVGLNNHRQTVIFGATLLYDETIPSFQWLFETFLKAMGREKPKTILTDQDAAMAKAISLVMPKTFHGLCTWHIRQNALRHVNHLYQKSSRFRLDFEACIYLHEEESEFLNEWNSLLVDHNVLKDSWLHSIFKLKEKWAWAYVRKTFTAGMRSTQLSESFNADLKNHLKSDINLVQFFTHFKRAVNAKRNNESEAEYDSRHKLPKLKMKKARMLLQAANVYTPKIFEEFQEEYEEYQDTCIKEVKEGLYVVTNYDNAKERMVMGNPMEQKVSCDCHKFETHGILCSHALKVLDVMNIKLIPEHYILKRWTRDARLGSNQGWSGRHVELDQKAHFMKRYIELCPQLLKLTNRASESHETYIFLSKVYEESSKIVNDMLSKKYLDGESSGMVHVSISIANDEVDNNIDTVGKAKGIKTKDCPRKSKKRYMSWLEKERKKTKMRSEKKEKISRSNQATQFKLHNQAKRLVRFKNFDVKYHKSVCYNSDSEIFVLADPCVDSGVVVIIVQQLLLVLVEEALSKLLLLLCGCWCVSLHSSGVVRTIGVFRGLLARLCIMLSLFGFLLWSILILEKGGFLVLRLYAAPFKLARVKVGKSESTGADWCGREEQQGVLVCEEGGETRERGGDRKRGDDVFNRRRFLEAWRRGRRQKGGMATGGGAWDWRRPLWVWEEELVEECRQLLNGVVLQYDISDRWLWDSNNDDVYTVRGAYQILTTMADPPSVGVGDLVWHKQVPLNVSIMVWRLLRDRLPTKVNLVRRGCLDVEAAGCGAGCGIAETANHLFLHCSTFGALWQHIRAWIGVSGVDPHDLMEGDGFWLRVLVARYGAEDGRLEVGGQSCSSWWREIVRIRDGVRGSSDGWFATCVSRKVGDGAETSFWRDCWCGGVSFQDRFRRLYDLADNKAITVRNLFLLGWEVGGEALRWRRSLGVWEEELLEELASGPDRGYSVRGVYDMLTSQEHAQVHPSTKLIWHKQVPLKVSIFAWRLLRDRLATKSKLATRGVIATKARLCVFGCGHVEDAQHLFL*