AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0467620


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0467620 MtrunA17_Chr3g0122071 90.598 117 11 0 213 329 106 222 5.70e-73 225
Msa0467620 MtrunA17_Chr3g0122071 69.744 195 14 2 1 184 1 161 8.46e-54 176
Msa0467620 MtrunA17_Chr4g0064011 54.494 178 57 9 1 174 1 158 2.52e-42 146
Msa0467620 MtrunA17_Chr4g0064011 61.069 131 48 3 203 332 104 232 6.88e-39 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0467620 AT5G22990.1 51.807 83 40 0 220 302 232 314 1.02e-19 88.6
Msa0467620 AT5G22990.1 51.282 78 24 2 136 213 232 295 3.98e-16 78.6
Msa0467620 AT2G18490.1 53.425 73 33 1 207 279 142 213 6.15e-17 79.7
Msa0467620 AT2G18490.1 60.000 60 23 1 123 182 142 200 5.48e-15 73.9
Msa0467620 AT2G18490.2 53.425 73 33 1 207 279 192 263 7.52e-17 80.1
Msa0467620 AT2G18490.2 58.065 62 25 1 123 184 192 252 7.62e-15 74.3
Msa0467620 AT2G15740.1 52.564 78 32 2 209 286 219 291 1.14e-16 80.1
Msa0467620 AT2G15740.1 60.000 60 23 1 125 184 219 277 9.03e-16 77.4
Msa0467620 AT5G42640.1 52.113 71 29 2 213 279 218 287 3.16e-15 75.5
Msa0467620 AT5G42640.1 39.216 102 57 2 85 182 174 274 5.40e-13 68.9

Find 38 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATTAATATTTATATTAA+TGG 0.098823 3_3:-69058447 None:intergenic
TCAATTGGTACCAATAATTT+TGG 0.136685 3_3:+69058335 Msa0467620:CDS
AGCATGACTTCCAAAATTAT+TGG 0.299809 3_3:-69058345 None:intergenic
AGCCTACCTCACAAGAAATA+TGG 0.344617 3_3:+69058695 Msa0467620:CDS
AGCCTACCTCACAAGAAATA+TGG 0.344617 3_3:+69059047 Msa0467620:CDS
GAGATTCTTGAAGGTCAGTT+TGG 0.349001 3_3:+69058389 Msa0467620:CDS
ATCGTTCTTGTTCCGATGCA+TGG 0.373109 3_3:-69058973 None:intergenic
ATTTCTTGTGAGGTAGGCTA+TGG 0.375103 3_3:-69058691 None:intergenic
ATTTCTTGTGAGGTAGGCTA+TGG 0.375103 3_3:-69059043 None:intergenic
ACAATAATAGATTGTTTCAA+TGG 0.400008 3_3:+69058279 Msa0467620:CDS
TATTAATATTAATTATTTGA+AGG 0.413280 3_3:-69059359 None:intergenic
AGATTCTTGAAGGTCAGTTT+GGG 0.424623 3_3:+69058390 Msa0467620:CDS
TCGTTCTTGTTCCGATGCAT+GGG 0.447785 3_3:-69058972 None:intergenic
TTATCAGTACAAGCAATAAC+AGG 0.454560 3_3:-69059254 None:intergenic
ACTCATTCATCATCATCAAT+TGG 0.471297 3_3:+69058320 Msa0467620:CDS
AGCAATGAAAATATTGATGA+TGG 0.472887 3_3:+69058662 Msa0467620:CDS
AGCAATGAAAATATTGATGA+TGG 0.472887 3_3:+69059014 Msa0467620:CDS
ACATCTCCAACATCAGCAAC+AGG 0.474279 3_3:-69058608 None:intergenic
AATATTCCTGTTGCTGATGT+TGG 0.486932 3_3:+69058602 Msa0467620:CDS
TCTCATCTTGATCTCCGTGT+AGG 0.496262 3_3:+69058539 Msa0467620:CDS
AAAGCGATGGAGATTCTTGA+AGG 0.521307 3_3:+69058380 Msa0467620:CDS
CTCTTACCCCTCTTCCAAGT+GGG 0.526442 3_3:+69059318 Msa0467620:CDS
TTTCTTGTGAGGTAGGCTAT+GGG 0.526908 3_3:-69058690 None:intergenic
TTTCTTGTGAGGTAGGCTAT+GGG 0.526908 3_3:-69059042 None:intergenic
AGATTGTTTCAATGGATGCA+TGG 0.544477 3_3:+69058287 Msa0467620:CDS
ATGTTAACAAGAACGATATG+AGG 0.552379 3_3:+69058627 Msa0467620:CDS
GGAATATTAATCATTTGAGA+AGG 0.562733 3_3:-69058587 None:intergenic
ATCGGAACAAGAACGATATG+AGG 0.569024 3_3:+69058979 Msa0467620:CDS
TACGGTCCATATTTCTTGTG+AGG 0.577081 3_3:-69058701 None:intergenic
TACGGTCCATATTTCTTGTG+AGG 0.577081 3_3:-69059053 None:intergenic
GCTCTTACCCCTCTTCCAAG+TGG 0.581741 3_3:+69059317 Msa0467620:CDS
GTCCATATTTCTTGTGAGGT+AGG 0.582227 3_3:-69058697 None:intergenic
GTCCATATTTCTTGTGAGGT+AGG 0.582227 3_3:-69059049 None:intergenic
CAGATTCAAAAGCTCAATGA+CGG 0.593887 3_3:+69059215 Msa0467620:CDS
ATTGCTCTTACCCCATGCAT+CGG 0.621144 3_3:+69058961 Msa0467620:CDS
TCCGGGAATCCTAAAAGCGA+TGG 0.625731 3_3:+69058367 Msa0467620:CDS
CGTTCTTGTTCCGATGCATG+GGG 0.640029 3_3:-69058971 None:intergenic
ATTGTGATGATAAGCCTACA+CGG 0.769789 3_3:-69058553 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATAATAGATTGTTTCAA+TGG + chr3_3:69058279-69058298 Msa0467620:CDS 20.0%
!!! TTTTGTTACATTTTGAACAT+GGG - chr3_3:69059079-69059098 None:intergenic 20.0%
!!! ATTTTGTTACATTTTGAACA+TGG - chr3_3:69059080-69059099 None:intergenic 20.0%
!! ACAATAATAGATTGTTTCAA+TGG + chr3_3:69058279-69058298 Msa0467620:CDS 20.0%
!!! TTTTGTTACATTTTGAACAT+GGG - chr3_3:69059079-69059098 None:intergenic 20.0%
!!! ATTTTGTTACATTTTGAACA+TGG - chr3_3:69059080-69059099 None:intergenic 20.0%
!! TCAATTGGTACCAATAATTT+TGG + chr3_3:69058335-69058354 Msa0467620:CDS 25.0%
! GGAATATTAATCATTTGAGA+AGG - chr3_3:69058590-69058609 None:intergenic 25.0%
! AGCAATGAAAATATTGATGA+TGG + chr3_3:69058662-69058681 Msa0467620:CDS 25.0%
!! TTTTGTTGCATTTTGAACAT+GGG - chr3_3:69058727-69058746 None:intergenic 25.0%
!! ATTTTGTTGCATTTTGAACA+TGG - chr3_3:69058728-69058747 None:intergenic 25.0%
! AGAAAAAGAGATACATTTCT+AGG + chr3_3:69058818-69058837 Msa0467620:CDS 25.0%
! AGCAATGAAAATATTGATGA+TGG + chr3_3:69058662-69058681 Msa0467620:CDS 25.0%
! GAAAAAAGAGATACATTTCT+AGG + chr3_3:69059171-69059190 Msa0467620:CDS 25.0%
!! TCAATTGGTACCAATAATTT+TGG + chr3_3:69058335-69058354 Msa0467620:CDS 25.0%
! GGAATATTAATCATTTGAGA+AGG - chr3_3:69058590-69058609 None:intergenic 25.0%
! AGCAATGAAAATATTGATGA+TGG + chr3_3:69058662-69058681 Msa0467620:CDS 25.0%
!! TTTTGTTGCATTTTGAACAT+GGG - chr3_3:69058727-69058746 None:intergenic 25.0%
!! ATTTTGTTGCATTTTGAACA+TGG - chr3_3:69058728-69058747 None:intergenic 25.0%
! AGAAAAAGAGATACATTTCT+AGG + chr3_3:69058818-69058837 Msa0467620:CDS 25.0%
! AGCAATGAAAATATTGATGA+TGG + chr3_3:69058662-69058681 Msa0467620:CDS 25.0%
! GAAAAAAGAGATACATTTCT+AGG + chr3_3:69059171-69059190 Msa0467620:CDS 25.0%
ACTCATTCATCATCATCAAT+TGG + chr3_3:69058320-69058339 Msa0467620:CDS 30.0%
AGCATGACTTCCAAAATTAT+TGG - chr3_3:69058348-69058367 None:intergenic 30.0%
ATGTTAACAAGAACGATATG+AGG + chr3_3:69058627-69058646 Msa0467620:CDS 30.0%
TTATCAGTACAAGCAATAAC+AGG - chr3_3:69058904-69058923 None:intergenic 30.0%
! TTACATTTTGAACATGGGTA+CGG - chr3_3:69059074-69059093 None:intergenic 30.0%
TTATCAGTACAAGCAATAAC+AGG - chr3_3:69058904-69058923 None:intergenic 30.0%
AAGTGGGATAAAAATCAAGT+CGG + chr3_3:69059334-69059353 Msa0467620:CDS 30.0%
ACTCATTCATCATCATCAAT+TGG + chr3_3:69058320-69058339 Msa0467620:CDS 30.0%
AGCATGACTTCCAAAATTAT+TGG - chr3_3:69058348-69058367 None:intergenic 30.0%
ATGTTAACAAGAACGATATG+AGG + chr3_3:69058627-69058646 Msa0467620:CDS 30.0%
TTATCAGTACAAGCAATAAC+AGG - chr3_3:69058904-69058923 None:intergenic 30.0%
! TTACATTTTGAACATGGGTA+CGG - chr3_3:69059074-69059093 None:intergenic 30.0%
TTATCAGTACAAGCAATAAC+AGG - chr3_3:69058904-69058923 None:intergenic 30.0%
AAGTGGGATAAAAATCAAGT+CGG + chr3_3:69059334-69059353 Msa0467620:CDS 30.0%
AGATTGTTTCAATGGATGCA+TGG + chr3_3:69058287-69058306 Msa0467620:CDS 35.0%
!! TAATTTTGGAAGTCATGCTC+CGG + chr3_3:69058349-69058368 Msa0467620:CDS 35.0%
AGATTCTTGAAGGTCAGTTT+GGG + chr3_3:69058390-69058409 Msa0467620:CDS 35.0%
ATTGTGATGATAAGCCTACA+CGG - chr3_3:69058556-69058575 None:intergenic 35.0%
AATATTCCTGTTGCTGATGT+TGG + chr3_3:69058602-69058621 Msa0467620:CDS 35.0%
! TTGCATTTTGAACATGGGTA+CGG - chr3_3:69058722-69058741 None:intergenic 35.0%
CAGATTCAAAAGCTCAATGA+CGG + chr3_3:69058862-69058881 Msa0467620:intron 35.0%
CAGATTCAAAAGCTCAATGA+CGG + chr3_3:69058862-69058881 Msa0467620:intron 35.0%
! TTTTTATCCCACTTGGAAGA+GGG - chr3_3:69059328-69059347 None:intergenic 35.0%
! ATTTTTATCCCACTTGGAAG+AGG - chr3_3:69059329-69059348 None:intergenic 35.0%
! GACTTGATTTTTATCCCACT+TGG - chr3_3:69059335-69059354 None:intergenic 35.0%
AGATTGTTTCAATGGATGCA+TGG + chr3_3:69058287-69058306 Msa0467620:CDS 35.0%
!! TAATTTTGGAAGTCATGCTC+CGG + chr3_3:69058349-69058368 Msa0467620:CDS 35.0%
AGATTCTTGAAGGTCAGTTT+GGG + chr3_3:69058390-69058409 Msa0467620:CDS 35.0%
ATTGTGATGATAAGCCTACA+CGG - chr3_3:69058556-69058575 None:intergenic 35.0%
AATATTCCTGTTGCTGATGT+TGG + chr3_3:69058602-69058621 Msa0467620:CDS 35.0%
! TTGCATTTTGAACATGGGTA+CGG - chr3_3:69058722-69058741 None:intergenic 35.0%
CAGATTCAAAAGCTCAATGA+CGG + chr3_3:69058862-69058881 Msa0467620:intron 35.0%
CAGATTCAAAAGCTCAATGA+CGG + chr3_3:69058862-69058881 Msa0467620:intron 35.0%
! TTTTTATCCCACTTGGAAGA+GGG - chr3_3:69059328-69059347 None:intergenic 35.0%
! ATTTTTATCCCACTTGGAAG+AGG - chr3_3:69059329-69059348 None:intergenic 35.0%
! GACTTGATTTTTATCCCACT+TGG - chr3_3:69059335-69059354 None:intergenic 35.0%
!! AATTTTGGAAGTCATGCTCC+GGG + chr3_3:69058350-69058369 Msa0467620:CDS 40.0%
!! CAAGAATCTCCATCGCTTTT+AGG - chr3_3:69058379-69058398 None:intergenic 40.0%
AAAGCGATGGAGATTCTTGA+AGG + chr3_3:69058380-69058399 Msa0467620:CDS 40.0%
GAGATTCTTGAAGGTCAGTT+TGG + chr3_3:69058389-69058408 Msa0467620:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG - chr3_3:69058693-69058712 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG - chr3_3:69058694-69058713 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG + chr3_3:69058695-69058714 Msa0467620:CDS 40.0%
GTCCATATTTCTTGTGAGGT+AGG - chr3_3:69058700-69058719 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG - chr3_3:69058704-69058723 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG - chr3_3:69058856-69058875 None:intergenic 40.0%
ATCGGAACAAGAACGATATG+AGG + chr3_3:69058979-69058998 Msa0467620:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG - chr3_3:69058693-69058712 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG - chr3_3:69058694-69058713 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG + chr3_3:69058695-69058714 Msa0467620:CDS 40.0%
GTCCATATTTCTTGTGAGGT+AGG - chr3_3:69058700-69058719 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG - chr3_3:69058704-69058723 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG - chr3_3:69058856-69058875 None:intergenic 40.0%
! TTTTATCCCACTTGGAAGAG+GGG - chr3_3:69059327-69059346 None:intergenic 40.0%
!! AATTTTGGAAGTCATGCTCC+GGG + chr3_3:69058350-69058369 Msa0467620:CDS 40.0%
!! CAAGAATCTCCATCGCTTTT+AGG - chr3_3:69058379-69058398 None:intergenic 40.0%
AAAGCGATGGAGATTCTTGA+AGG + chr3_3:69058380-69058399 Msa0467620:CDS 40.0%
GAGATTCTTGAAGGTCAGTT+TGG + chr3_3:69058389-69058408 Msa0467620:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG - chr3_3:69058693-69058712 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG - chr3_3:69058694-69058713 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG + chr3_3:69058695-69058714 Msa0467620:CDS 40.0%
GTCCATATTTCTTGTGAGGT+AGG - chr3_3:69058700-69058719 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG - chr3_3:69058704-69058723 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG - chr3_3:69058856-69058875 None:intergenic 40.0%
ATCGGAACAAGAACGATATG+AGG + chr3_3:69058979-69058998 Msa0467620:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG - chr3_3:69058693-69058712 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG - chr3_3:69058694-69058713 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG + chr3_3:69058695-69058714 Msa0467620:CDS 40.0%
GTCCATATTTCTTGTGAGGT+AGG - chr3_3:69058700-69058719 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG - chr3_3:69058704-69058723 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG - chr3_3:69058856-69058875 None:intergenic 40.0%
! TTTTATCCCACTTGGAAGAG+GGG - chr3_3:69059327-69059346 None:intergenic 40.0%
!!! TCCATCGCTTTTAGGATTCC+CGG - chr3_3:69058371-69058390 None:intergenic 45.0%
TCTCATCTTGATCTCCGTGT+AGG + chr3_3:69058539-69058558 Msa0467620:CDS 45.0%
ACATCTCCAACATCAGCAAC+AGG - chr3_3:69058611-69058630 None:intergenic 45.0%
ATTGCTCTTACCCCATGCAT+CGG + chr3_3:69058961-69058980 Msa0467620:CDS 45.0%
TCGTTCTTGTTCCGATGCAT+GGG - chr3_3:69058975-69058994 None:intergenic 45.0%
ATCGTTCTTGTTCCGATGCA+TGG - chr3_3:69058976-69058995 None:intergenic 45.0%
!!! TCCATCGCTTTTAGGATTCC+CGG - chr3_3:69058371-69058390 None:intergenic 45.0%
TCTCATCTTGATCTCCGTGT+AGG + chr3_3:69058539-69058558 Msa0467620:CDS 45.0%
ACATCTCCAACATCAGCAAC+AGG - chr3_3:69058611-69058630 None:intergenic 45.0%
ATTGCTCTTACCCCATGCAT+CGG + chr3_3:69058961-69058980 Msa0467620:CDS 45.0%
TCGTTCTTGTTCCGATGCAT+GGG - chr3_3:69058975-69058994 None:intergenic 45.0%
ATCGTTCTTGTTCCGATGCA+TGG - chr3_3:69058976-69058995 None:intergenic 45.0%
!! ATCATTAATATTTATATTAA+TGG - chr3_3:69058450-69058469 None:intergenic 5.0%
!! ATCATTAATATTTATATTAA+TGG - chr3_3:69058450-69058469 None:intergenic 5.0%
TCCGGGAATCCTAAAAGCGA+TGG + chr3_3:69058367-69058386 Msa0467620:CDS 50.0%
CGTTCTTGTTCCGATGCATG+GGG - chr3_3:69058974-69058993 None:intergenic 50.0%
CTCTTACCCCTCTTCCAAGT+GGG + chr3_3:69059318-69059337 Msa0467620:CDS 50.0%
TCCGGGAATCCTAAAAGCGA+TGG + chr3_3:69058367-69058386 Msa0467620:CDS 50.0%
CGTTCTTGTTCCGATGCATG+GGG - chr3_3:69058974-69058993 None:intergenic 50.0%
CTCTTACCCCTCTTCCAAGT+GGG + chr3_3:69059318-69059337 Msa0467620:CDS 50.0%
GCTCTTACCCCTCTTCCAAG+TGG + chr3_3:69059317-69059336 Msa0467620:CDS 55.0%
GCTCTTACCCCTCTTCCAAG+TGG + chr3_3:69059317-69059336 Msa0467620:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 69058275 69059373 69058275 ID=Msa0467620;Name=Msa0467620
chr3_3 mRNA 69058275 69059373 69058275 ID=Msa0467620-mRNA-1;Parent=Msa0467620;Name=Msa0467620-mRNA-1;_AED=0.07;_eAED=0.10;_QI=0|0|0|1|1|1|2|0|332
chr3_3 exon 69058275 69058856 69058275 ID=Msa0467620-mRNA-1:exon:16174;Parent=Msa0467620-mRNA-1
chr3_3 exon 69058957 69059373 69058957 ID=Msa0467620-mRNA-1:exon:16175;Parent=Msa0467620-mRNA-1
chr3_3 CDS 69058275 69058856 69058275 ID=Msa0467620-mRNA-1:cds;Parent=Msa0467620-mRNA-1
chr3_3 CDS 69058957 69059373 69058957 ID=Msa0467620-mRNA-1:cds;Parent=Msa0467620-mRNA-1
Gene Sequence

>Msa0467620

ATGAACAATAATAGATTGTTTCAATGGATGCATGGTTACAACACAACTCATTCATCATCATCAATTGGTACCAATAATTTTGGAAGTCATGCTCCGGGAATCCTAAAAGCGATGGAGATTCTTGAAGGTCAGTTTGGGCATTTGCATACATCAACATCATCATCGTCATCATCCATTAATATAAATATTAATGATGACATCATCATAAATAACAACAAGAACAACAACATGATGATGACAATGAATACTTCTGCTGCTGATTTTTCTCATCTTGATCTCCGTGTAGGCTTATCATCACAATCACAAGAACTGCCTTCTCAAATGATTAATATTCCTGTTGCTGATGTTGGAGATGTTAACAAGAACGATATGAGGAAGAAGAAGAAGAGCAATGAAAATATTGATGATGGAAGAACCCATAGCCTACCTCACAAGAAATATGGACCGTACCCATGTTCAAAATGCAACAAAATATTTGAAACATCACAAAAATTTGCTAATCATGTTTCATCAAGTCACTACAAGTTCGAAAGTGAAGAAGACAGAAAAAGAGATACATTTCTAGGATTCAGAAGAGACCGCTATGATTGCTCTTACCCCATGCATCGGAACAAGAACGATATGAGGAAGAAGAAGAAGAGCAATGAAAATATTGATGATGGAAGAACCCATAGCCTACCTCACAAGAAATATGGACCGTACCCATGTTCAAAATGTAACAAAATATTTGAAACATCACAAAAATTTGCTAATCATGTTTCATCAAGTCACTACAAGTTCGAAAGTGAAGAAGACAGAAAAAAGAGATACATTTCTAGGATTCAGAAGAGACCGCGTCTTCAGATTCAAAAGCTCAATGACGGAACAACTACTTTTGTTCCTGTTATTGCTTGTACTGATAAGTCACATGCTTCAGTCAATGATGATGATCATAGTATGATTGCTCTTACCCCTCTTCCAAGTGGGATAAAAATCAAGTCGGAGCCTTCAAATAATTAA

Protein sequence

>Msa0467620

MNNNRLFQWMHGYNTTHSSSSIGTNNFGSHAPGILKAMEILEGQFGHLHTSTSSSSSSINININDDIIINNNKNNNMMMTMNTSAADFSHLDLRVGLSSQSQELPSQMINIPVADVGDVNKNDMRKKKKSNENIDDGRTHSLPHKKYGPYPCSKCNKIFETSQKFANHVSSSHYKFESEEDRKRDTFLGFRRDRYDCSYPMHRNKNDMRKKKKSNENIDDGRTHSLPHKKYGPYPCSKCNKIFETSQKFANHVSSSHYKFESEEDRKKRYISRIQKRPRLQIQKLNDGTTTFVPVIACTDKSHASVNDDDHSMIALTPLPSGIKIKSEPSNN*