AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0474410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0474410 MtrunA17_Chr3g0131181 74.000 100 26 0 3 102 184 283 1.71e-50 167
Msa0474410 MtrunA17_Chr8g0385341 71.717 99 28 0 3 101 185 283 1.54e-46 156
Msa0474410 MtrunA17_Chr1g0170281 66.667 102 34 0 1 102 271 372 2.63e-44 151
Msa0474410 MtrunA17_Chr1g0200601 64.000 100 36 0 3 102 185 284 4.76e-43 146
Msa0474410 MtrunA17_Chr1g0176421 66.000 100 34 0 3 102 316 415 5.87e-43 148
Msa0474410 MtrunA17_Chr5g0448721 63.000 100 37 0 3 102 251 350 1.28e-41 144
Msa0474410 MtrunA17_Chr7g0242561 60.606 99 39 0 3 101 211 309 7.51e-41 141
Msa0474410 MtrunA17_Chr4g0059951 63.636 99 36 0 3 101 228 326 1.13e-40 141
Msa0474410 MtrunA17_Chr7g0257751 57.576 99 42 0 3 101 213 311 3.75e-40 139
Msa0474410 MtrunA17_Chr7g0267771 59.000 100 41 0 3 102 243 342 7.76e-40 138
Msa0474410 MtrunA17_Chr5g0444191 60.606 99 39 0 1 99 61 159 1.58e-39 130
Msa0474410 MtrunA17_Chr1g0176751 63.636 99 36 0 3 101 279 377 2.15e-39 138
Msa0474410 MtrunA17_Chr5g0444171 60.396 101 40 0 2 102 246 346 9.58e-39 135
Msa0474410 MtrunA17_Chr1g0173541 56.000 100 44 0 3 102 207 306 9.63e-39 135
Msa0474410 MtrunA17_Chr1g0157131 62.000 100 38 0 2 101 228 327 1.02e-38 135
Msa0474410 MtrunA17_Chr3g0098951 62.000 100 38 0 2 101 238 337 3.48e-38 134
Msa0474410 MtrunA17_Chr4g0074811 62.000 100 38 0 2 101 245 344 4.01e-38 134
Msa0474410 MtrunA17_Chr0c02g0489761 60.396 101 40 0 2 102 232 332 6.79e-38 133
Msa0474410 MtrunA17_Chr7g0267751 58.000 100 42 0 3 102 290 389 6.21e-37 131
Msa0474410 MtrunA17_Chr4g0033371 51.376 109 53 0 1 109 225 333 9.58e-37 130
Msa0474410 MtrunA17_Chr5g0441691 56.436 101 44 0 2 102 222 322 2.80e-36 129
Msa0474410 MtrunA17_Chr5g0397101 58.416 101 42 0 4 104 223 323 4.47e-36 128
Msa0474410 MtrunA17_Chr8g0387671 57.000 100 43 0 3 102 221 320 5.11e-36 128
Msa0474410 MtrunA17_Chr4g0074791 56.311 103 45 0 2 104 250 352 4.53e-33 120
Msa0474410 MtrunA17_Chr5g0405421 50.000 100 50 0 3 102 256 355 5.29e-31 114
Msa0474410 MtrunA17_Chr1g0203881 53.535 99 46 0 3 101 196 294 5.19e-29 108
Msa0474410 MtrunA17_Chr1g0190131 48.039 102 52 1 2 102 159 260 1.17e-28 104
Msa0474410 MtrunA17_Chr8g0374701 45.918 98 53 0 4 101 288 385 1.25e-26 102
Msa0474410 MtrunA17_Chr7g0256001 46.465 99 53 0 4 102 301 399 3.56e-26 101
Msa0474410 MtrunA17_Chr4g0045261 45.455 99 54 0 4 102 297 395 5.20e-26 100
Msa0474410 MtrunA17_Chr5g0402551 45.455 99 54 0 4 102 294 392 7.72e-26 100
Msa0474410 MtrunA17_Chr3g0127571 45.455 99 54 0 4 102 298 396 1.24e-25 99.8
Msa0474410 MtrunA17_Chr7g0276111 44.444 99 55 0 4 102 304 402 1.97e-24 96.3
Msa0474410 MtrunA17_Chr3g0132591 44.000 100 56 0 2 101 256 355 3.31e-24 95.5
Msa0474410 MtrunA17_Chr7g0274941 40.594 101 58 1 3 101 169 269 1.40e-18 78.6
Msa0474410 MtrunA17_Chr8g0355131 39.785 93 55 1 11 102 210 302 7.58e-16 72.0
Msa0474410 MtrunA17_Chr6g0468751 40.594 101 54 2 2 100 169 265 2.05e-15 70.9
Msa0474410 MtrunA17_Chr6g0453681 40.594 101 54 2 2 100 169 265 2.19e-15 70.5
Msa0474410 MtrunA17_Chr8g0346071 42.157 102 53 3 2 101 168 265 2.27e-15 70.5
Msa0474410 MtrunA17_Chr6g0478601 41.935 93 44 3 10 97 301 388 2.81e-15 70.5
Msa0474410 MtrunA17_Chr2g0333821 44.681 94 46 3 10 101 174 263 7.67e-15 68.9
Msa0474410 MtrunA17_Chr5g0428031 40.426 94 48 3 10 100 168 256 1.12e-14 68.6
Msa0474410 MtrunA17_Chr4g0018201 43.750 96 50 3 2 95 940 1033 2.66e-14 67.8
Msa0474410 MtrunA17_Chr5g0428981 43.434 99 46 4 2 95 944 1037 3.63e-14 67.4
Msa0474410 MtrunA17_Chr4g0001331 41.053 95 50 3 10 102 180 270 3.71e-14 67.0
Msa0474410 MtrunA17_Chr4g0069301 35.484 93 54 3 10 100 187 275 3.94e-14 67.0
Msa0474410 MtrunA17_Chr5g0441011 40.625 96 51 3 9 102 173 264 4.09e-14 67.0
Msa0474410 MtrunA17_Chr4g0013131 38.776 98 54 3 9 104 171 264 4.35e-14 67.0
Msa0474410 MtrunA17_Chr2g0302431 37.634 93 52 3 10 100 181 269 6.36e-14 66.6
Msa0474410 MtrunA17_Chr4g0008561 40.000 90 51 3 9 96 178 266 7.20e-14 66.2
Msa0474410 MtrunA17_Chr3g0140631 36.170 94 57 2 9 101 192 283 2.17e-13 64.7
Msa0474410 MtrunA17_Chr1g0150321 38.947 95 52 3 10 102 173 263 4.38e-13 63.9
Msa0474410 MtrunA17_Chr1g0166101 40.816 98 52 3 9 104 184 277 4.78e-13 63.9
Msa0474410 MtrunA17_Chr3g0132671 35.714 98 55 3 2 96 199 291 5.42e-13 63.9
Msa0474410 MtrunA17_Chr7g0244561 35.789 95 55 3 9 101 177 267 5.59e-13 63.5
Msa0474410 MtrunA17_Chr1g0205421 36.842 95 54 3 9 101 171 261 6.98e-13 63.5
Msa0474410 MtrunA17_Chr6g0453671 38.000 100 56 2 3 100 170 265 7.43e-13 63.2
Msa0474410 MtrunA17_Chr8g0338741 34.694 98 58 2 4 100 245 337 1.10e-12 62.8
Msa0474410 MtrunA17_Chr2g0284011 36.082 97 56 3 10 104 172 264 2.58e-12 62.0
Msa0474410 MtrunA17_Chr3g0135951 41.772 79 45 1 10 87 302 380 2.59e-12 62.0
Msa0474410 MtrunA17_Chr1g0188031 40.000 90 47 3 10 96 182 267 4.56e-12 61.2
Msa0474410 MtrunA17_Chr5g0432471 36.170 94 54 3 9 100 171 260 6.76e-12 60.8
Msa0474410 MtrunA17_Chr7g0244521 36.082 97 56 2 10 104 172 264 7.49e-12 60.5
Msa0474410 MtrunA17_Chr2g0284031 33.673 98 59 2 6 101 187 280 1.02e-11 60.1
Msa0474410 MtrunA17_Chr4g0063881 37.634 93 52 3 4 94 164 252 1.48e-11 59.7
Msa0474410 MtrunA17_Chr2g0299781 37.209 86 53 1 9 93 320 405 2.97e-11 58.9
Msa0474410 MtrunA17_Chr8g0380661 33.333 99 60 3 9 102 156 253 3.61e-11 58.5
Msa0474410 MtrunA17_Chr1g0153321 42.500 80 45 1 9 87 308 387 3.64e-11 58.5
Msa0474410 MtrunA17_Chr1g0187581 40.909 88 50 2 9 95 316 402 3.80e-11 58.5
Msa0474410 MtrunA17_Chr1g0183651 40.000 80 47 1 9 87 318 397 5.16e-11 58.2
Msa0474410 MtrunA17_Chr7g0261491 40.000 80 47 1 9 87 318 397 7.20e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0474410 AT5G04870.1 67.677 99 32 0 4 102 306 404 5.29e-44 151
Msa0474410 AT5G23580.1 65.000 100 35 0 3 102 177 276 5.64e-44 149
Msa0474410 AT2G35890.2 67.000 100 33 0 3 102 287 386 1.37e-43 149
Msa0474410 AT2G35890.1 67.000 100 33 0 3 102 287 386 1.37e-43 149
Msa0474410 AT3G10660.2 66.667 99 33 0 4 102 342 440 2.36e-43 149
Msa0474410 AT3G10660.1 66.667 99 33 0 4 102 342 440 2.36e-43 149
Msa0474410 AT2G38910.1 64.000 100 36 0 3 102 289 388 1.43e-42 147
Msa0474410 AT1G35670.1 65.000 100 35 0 3 102 181 280 2.22e-42 145
Msa0474410 AT4G09570.1 64.000 100 36 0 3 102 180 279 2.28e-42 145
Msa0474410 AT4G23650.1 64.000 100 36 0 3 102 233 332 5.94e-42 144
Msa0474410 AT3G51850.2 60.606 99 39 0 3 101 209 307 1.40e-41 143
Msa0474410 AT4G38230.1 62.626 99 37 0 3 101 35 133 1.61e-41 140
Msa0474410 AT2G17290.1 63.636 99 36 0 3 101 240 338 1.80e-41 143
Msa0474410 AT2G17290.2 63.636 99 36 0 3 101 240 338 1.80e-41 143
Msa0474410 AT3G51850.1 60.606 99 39 0 3 101 209 307 2.60e-41 143
Msa0474410 AT4G35310.2 62.626 99 37 0 3 101 252 350 8.00e-41 142
Msa0474410 AT4G35310.1 62.626 99 37 0 3 101 252 350 8.00e-41 142
Msa0474410 AT4G38230.4 62.626 99 37 0 3 101 179 277 1.47e-40 140
Msa0474410 AT4G38230.3 62.626 99 37 0 3 101 179 277 1.47e-40 140
Msa0474410 AT5G12480.2 58.824 102 42 0 3 104 120 221 1.69e-40 139
Msa0474410 AT3G51850.3 60.606 99 39 0 3 101 209 307 2.03e-40 139
Msa0474410 AT4G38230.2 62.626 99 37 0 3 101 209 307 2.86e-40 140
Msa0474410 AT5G12480.1 58.824 102 42 0 3 104 214 315 4.72e-40 139
Msa0474410 AT1G76040.1 59.596 99 40 0 3 101 29 127 5.74e-40 135
Msa0474410 AT1G76040.4 59.596 99 40 0 3 101 29 127 5.74e-40 135
Msa0474410 AT5G19450.2 58.824 102 42 0 3 104 212 313 3.68e-39 137
Msa0474410 AT5G19450.1 58.824 102 42 0 3 104 212 313 3.68e-39 137
Msa0474410 AT1G76040.3 60.000 100 40 0 2 101 266 365 5.48e-39 136
Msa0474410 AT3G57530.2 58.000 100 42 0 3 102 218 317 6.33e-39 135
Msa0474410 AT1G76040.2 60.000 100 40 0 2 101 266 365 7.92e-39 136
Msa0474410 AT3G57530.1 58.000 100 42 0 3 102 218 317 1.95e-38 135
Msa0474410 AT2G41860.1 59.000 100 41 0 3 102 209 308 3.90e-38 134
Msa0474410 AT1G50700.3 58.000 100 42 0 2 101 227 326 6.81e-38 133
Msa0474410 AT1G61950.1 60.000 100 40 0 2 101 253 352 8.12e-38 134
Msa0474410 AT1G50700.1 58.000 100 42 0 2 101 227 326 8.88e-38 133
Msa0474410 AT1G50700.2 58.000 100 42 0 2 101 227 326 1.04e-37 133
Msa0474410 AT2G41860.2 58.000 100 42 0 3 102 209 308 1.10e-37 133
Msa0474410 AT4G04720.2 59.000 100 41 0 2 101 234 333 1.29e-37 133
Msa0474410 AT4G04720.1 59.000 100 41 0 2 101 234 333 1.29e-37 133
Msa0474410 AT3G20410.2 58.000 100 42 0 2 101 245 344 4.04e-37 132
Msa0474410 AT3G20410.1 58.000 100 42 0 2 101 245 344 4.04e-37 132
Msa0474410 AT4G04740.2 59.000 100 41 0 2 101 223 322 5.22e-37 131
Msa0474410 AT4G04740.4 59.000 100 41 0 2 101 223 322 5.62e-37 131
Msa0474410 AT4G04740.6 59.000 100 41 0 2 101 223 322 5.62e-37 131
Msa0474410 AT4G04740.5 59.000 100 41 0 2 101 223 322 5.62e-37 131
Msa0474410 AT4G04740.3 59.000 100 41 0 2 101 223 322 5.62e-37 131
Msa0474410 AT4G04740.1 59.000 100 41 0 2 101 223 322 5.62e-37 131
Msa0474410 AT4G04740.7 59.000 100 41 0 2 101 223 322 5.62e-37 131
Msa0474410 AT4G21940.3 56.000 100 44 0 2 101 256 355 1.21e-36 130
Msa0474410 AT5G12180.1 60.000 100 40 0 3 102 228 327 1.25e-36 130
Msa0474410 AT4G21940.2 56.000 100 44 0 2 101 256 355 1.76e-36 130
Msa0474410 AT1G74740.1 58.000 100 42 0 3 102 214 313 1.86e-36 130
Msa0474410 AT4G21940.1 56.000 100 44 0 2 101 256 355 1.97e-36 130
Msa0474410 AT5G19360.1 60.000 100 40 0 3 102 223 322 2.85e-36 129
Msa0474410 AT1G18890.1 56.000 100 44 0 3 102 218 317 3.73e-36 129
Msa0474410 AT2G31500.1 54.545 99 45 0 3 101 221 319 3.11e-35 127
Msa0474410 AT5G66210.6 49.020 102 52 0 3 104 219 320 9.61e-31 113
Msa0474410 AT5G66210.4 49.020 102 52 0 3 104 219 320 9.61e-31 113
Msa0474410 AT5G66210.5 49.020 102 52 0 3 104 219 320 1.26e-30 113
Msa0474410 AT2G17890.1 50.000 100 50 0 3 102 265 364 1.32e-30 114
Msa0474410 AT5G66210.3 49.020 102 52 0 3 104 219 320 1.59e-30 113
Msa0474410 AT5G66210.1 49.020 102 52 0 3 104 219 320 2.39e-30 113
Msa0474410 AT5G66210.2 49.020 102 52 0 3 104 219 320 2.39e-30 113
Msa0474410 AT4G04710.2 53.636 110 41 2 2 107 194 297 2.50e-30 112
Msa0474410 AT4G04710.3 53.636 110 41 2 2 107 180 283 2.67e-30 112
Msa0474410 AT1G08650.2 51.961 102 48 1 2 102 168 269 3.29e-30 109
Msa0474410 AT4G04710.1 51.282 117 46 2 2 107 194 310 7.02e-30 111
Msa0474410 AT4G04710.4 51.282 117 46 2 2 107 194 310 7.11e-30 111
Msa0474410 AT1G08650.1 51.485 101 48 1 2 101 168 268 1.56e-29 107
Msa0474410 AT1G12580.1 51.961 102 49 0 3 104 199 300 2.27e-29 110
Msa0474410 AT4G36070.1 48.039 102 53 0 3 104 228 329 2.74e-29 110
Msa0474410 AT4G36070.2 48.039 102 53 0 3 104 228 329 2.84e-29 110
Msa0474410 AT3G04530.1 52.427 103 48 1 3 104 165 267 6.94e-28 103
Msa0474410 AT4G04695.1 48.000 100 52 0 2 101 186 285 2.19e-26 102
Msa0474410 AT3G50530.2 45.455 99 54 0 4 102 339 437 3.99e-26 101
Msa0474410 AT3G50530.1 45.455 99 54 0 4 102 308 406 4.72e-26 101
Msa0474410 AT1G12680.1 47.000 100 53 0 2 101 251 350 9.74e-26 100
Msa0474410 AT3G19100.1 45.918 98 53 0 4 101 304 401 3.19e-25 99.0
Msa0474410 AT2G41140.1 44.444 99 55 0 4 102 283 381 7.08e-25 98.2
Msa0474410 AT4G04700.1 45.000 100 55 0 2 101 186 285 8.76e-25 97.4
Msa0474410 AT3G56760.1 43.434 99 56 0 4 102 284 382 1.34e-24 97.4
Msa0474410 AT5G24430.1 44.444 99 55 0 4 102 303 401 4.60e-24 95.9
Msa0474410 AT1G49580.1 42.857 98 56 0 4 101 310 407 6.66e-24 95.5
Msa0474410 AT2G46700.2 43.434 99 56 0 4 102 122 220 6.88e-24 94.7
Msa0474410 AT2G46700.3 43.434 99 56 0 4 102 122 220 6.88e-24 94.7
Msa0474410 AT3G49370.1 45.455 99 54 0 4 102 302 400 7.14e-24 95.1
Msa0474410 AT2G46700.1 43.434 99 56 0 4 102 303 401 3.36e-23 93.2
Msa0474410 AT1G45160.1 40.952 105 52 3 9 108 855 954 2.84e-17 76.6
Msa0474410 AT1G45160.2 40.952 105 52 3 9 108 855 954 2.87e-17 76.6
Msa0474410 AT5G21326.1 44.681 94 46 3 10 101 174 263 4.92e-16 72.8
Msa0474410 AT5G62310.1 45.745 94 41 3 9 97 943 1031 6.65e-16 72.8
Msa0474410 AT4G18700.1 40.594 101 54 3 6 104 182 278 9.01e-16 72.0
Msa0474410 AT3G01090.2 41.584 101 53 2 2 100 192 288 9.62e-16 72.0
Msa0474410 AT3G01090.3 41.584 101 53 2 2 100 169 265 1.01e-15 72.0
Msa0474410 AT3G01090.1 41.584 101 53 2 2 100 169 265 1.01e-15 72.0
Msa0474410 AT5G01810.1 39.583 96 52 3 11 104 173 264 1.18e-15 71.6
Msa0474410 AT5G01810.2 39.583 96 52 3 11 104 173 264 1.18e-15 71.6
Msa0474410 AT1G30270.3 40.000 95 51 3 9 101 94 184 2.16e-15 70.9
Msa0474410 AT5G45810.1 39.604 101 55 3 6 104 184 280 2.20e-15 70.9
Msa0474410 AT1G30270.1 40.000 95 51 3 9 101 191 281 2.22e-15 70.9
Msa0474410 AT1G30270.2 40.000 95 51 3 9 101 191 281 2.48e-15 70.9
Msa0474410 AT5G21222.1 41.489 94 49 3 10 101 174 263 3.35e-15 70.5
Msa0474410 AT5G21222.2 41.489 94 49 3 10 101 174 263 3.35e-15 70.5
Msa0474410 AT5G21222.4 41.489 94 49 3 10 101 174 263 3.35e-15 70.5
Msa0474410 AT5G21222.3 41.489 94 49 3 10 101 174 263 3.35e-15 70.5
Msa0474410 AT2G26980.5 42.553 94 48 3 10 101 175 264 3.99e-15 70.1
Msa0474410 AT5G10930.1 40.426 94 50 3 10 101 173 262 4.32e-15 70.1
Msa0474410 AT5G01810.3 39.175 97 53 3 10 104 172 264 4.83e-15 69.7
Msa0474410 AT2G26980.1 42.553 94 48 3 10 101 175 264 4.86e-15 69.7
Msa0474410 AT2G26980.3 42.553 94 48 3 10 101 175 264 5.34e-15 69.7
Msa0474410 AT2G26980.4 42.553 94 48 3 10 101 185 274 5.70e-15 69.7
Msa0474410 AT3G29160.3 42.268 97 50 2 2 96 170 262 6.29e-15 69.3
Msa0474410 AT5G39440.1 38.000 100 57 2 2 100 169 264 7.02e-15 69.7
Msa0474410 AT3G29160.1 42.268 97 50 2 2 96 170 262 7.49e-15 69.7
Msa0474410 AT3G29160.2 42.268 97 50 2 2 96 170 262 7.49e-15 69.7
Msa0474410 AT4G30960.1 39.362 94 51 3 10 101 184 273 8.04e-15 69.3
Msa0474410 AT5G58380.1 39.796 98 53 3 9 104 171 264 1.12e-14 68.9
Msa0474410 AT1G48260.1 43.333 90 43 3 10 96 172 256 1.83e-14 68.6
Msa0474410 AT1G48260.2 42.857 91 44 3 9 96 86 171 1.90e-14 68.2
Msa0474410 AT1G48260.4 42.857 91 44 3 9 96 86 171 1.90e-14 68.2
Msa0474410 AT1G48260.3 42.857 91 44 3 9 96 86 171 1.90e-14 68.2
Msa0474410 AT1G48260.5 42.857 91 44 3 9 96 86 171 1.90e-14 68.2
Msa0474410 AT4G24400.2 41.489 94 47 3 10 100 168 256 2.08e-14 68.2
Msa0474410 AT4G24400.1 41.489 94 47 3 10 100 168 256 2.62e-14 67.8
Msa0474410 AT1G01140.2 38.298 94 54 2 10 101 180 271 3.13e-14 67.8
Msa0474410 AT2G25090.1 39.130 92 50 3 11 100 185 272 3.52e-14 67.4
Msa0474410 AT2G26980.2 44.048 84 41 3 10 91 175 254 6.92e-14 65.9
Msa0474410 AT5G07070.1 39.796 98 53 3 9 104 171 264 9.08e-14 66.2
Msa0474410 AT1G01140.3 39.362 94 51 3 10 101 180 269 1.10e-13 66.2
Msa0474410 AT1G01140.1 39.362 94 51 3 10 101 180 269 1.12e-13 66.2
Msa0474410 AT3G17510.2 37.963 108 57 4 10 112 101 203 2.08e-13 65.1
Msa0474410 AT3G17850.1 37.255 102 54 3 1 97 1063 1159 2.18e-13 65.5
Msa0474410 AT1G48490.3 41.758 91 49 3 9 97 1011 1099 3.37e-13 65.1
Msa0474410 AT1G48490.2 41.758 91 49 3 9 97 1011 1099 3.37e-13 65.1
Msa0474410 AT1G48490.1 41.758 91 49 3 9 97 1011 1099 3.37e-13 65.1
Msa0474410 AT1G48490.4 41.758 91 49 3 9 97 1012 1100 3.40e-13 64.7
Msa0474410 AT3G17510.1 39.560 91 47 3 10 97 181 266 3.63e-13 64.7
Msa0474410 AT2G25880.2 36.170 94 57 2 9 101 148 239 3.99e-13 63.9
Msa0474410 AT2G25880.4 36.170 94 57 2 9 101 193 284 4.41e-13 63.9
Msa0474410 AT2G25880.1 36.170 94 57 2 9 101 180 271 5.04e-13 63.9
Msa0474410 AT2G25880.3 36.170 94 57 2 9 101 241 332 5.76e-13 63.9
Msa0474410 AT5G25110.1 40.449 89 47 3 10 96 203 287 8.43e-13 63.5
Msa0474410 AT1G29230.1 38.298 94 52 4 10 101 234 323 9.56e-13 63.5
Msa0474410 AT5G57630.3 34.951 103 61 2 3 103 74 172 1.16e-12 63.2
Msa0474410 AT5G09890.1 43.750 80 44 1 9 87 304 383 1.24e-12 63.2
Msa0474410 AT5G09890.2 43.750 80 44 1 9 87 304 383 1.25e-12 63.2
Msa0474410 AT3G61960.1 38.542 96 54 3 3 96 166 258 1.60e-12 62.8
Msa0474410 AT3G61960.2 38.542 96 54 3 3 96 166 258 1.62e-12 62.8
Msa0474410 AT5G57630.1 34.951 103 61 2 3 103 162 260 1.86e-12 62.8
Msa0474410 AT5G57630.2 34.951 103 61 2 3 103 162 260 1.94e-12 62.4
Msa0474410 AT5G45820.1 35.052 97 57 2 10 104 172 264 2.14e-12 62.4
Msa0474410 AT1G03920.2 41.250 80 46 1 9 87 222 301 2.43e-12 62.4
Msa0474410 AT1G03920.1 41.250 80 46 1 9 87 335 414 2.71e-12 62.4
Msa0474410 AT1G03920.3 41.250 80 46 1 9 87 335 414 2.71e-12 62.4
Msa0474410 AT3G53930.2 36.559 93 50 3 9 96 179 267 4.50e-12 61.6
Msa0474410 AT3G53930.1 36.559 93 50 3 9 96 179 267 4.54e-12 61.6
Msa0474410 AT5G35410.1 38.298 94 52 3 4 95 164 253 8.38e-12 60.8
Msa0474410 AT5G35410.2 38.298 94 52 3 4 95 164 253 9.13e-12 60.5
Msa0474410 AT1G30640.3 39.773 88 51 2 9 95 230 316 1.01e-11 60.5
Msa0474410 AT2G34180.1 36.170 94 54 2 10 101 217 306 1.06e-11 60.5
Msa0474410 AT1G30640.2 39.773 88 51 2 9 95 323 409 1.37e-11 60.1
Msa0474410 AT1G30640.1 39.773 88 51 2 9 95 323 409 1.37e-11 60.1
Msa0474410 AT1G60940.1 40.659 91 50 3 10 96 160 250 1.39e-11 60.1
Msa0474410 AT1G60940.2 40.659 91 50 3 10 96 160 250 1.39e-11 60.1
Msa0474410 AT3G25250.1 35.294 102 54 4 3 100 230 323 1.65e-11 60.1
Msa0474410 AT4G33080.2 41.772 79 45 1 10 87 302 380 4.00e-11 58.9
Msa0474410 AT4G33080.1 41.772 79 45 1 10 87 302 380 4.34e-11 58.9
Msa0474410 AT4G14350.3 40.909 88 50 2 9 95 318 404 5.52e-11 58.5
Msa0474410 AT2G20470.3 40.506 79 46 1 10 87 322 400 5.66e-11 58.5
Msa0474410 AT4G14350.2 40.909 88 50 2 9 95 318 404 6.51e-11 58.2
Msa0474410 AT4G14350.1 40.909 88 50 2 9 95 318 404 6.51e-11 58.2
Msa0474410 AT4G14350.4 40.909 88 50 2 9 95 318 404 6.51e-11 58.2
Msa0474410 AT2G19400.1 37.363 91 55 2 9 98 312 401 6.67e-11 58.2
Msa0474410 AT2G20470.2 40.000 80 47 1 9 87 321 400 8.01e-11 58.2
Msa0474410 AT2G20470.1 40.000 80 47 1 9 87 321 400 8.01e-11 58.2

Find 30 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGCGGGGTGCCACCGTTT+TGG 0.252792 3_3:+77324510 Msa0474410:CDS
AATAAGATACTTGCTCTATA+AGG 0.327797 3_3:-77324810 None:intergenic
CTGTTTGTTGATCCCCTCCT+TGG 0.331288 3_3:-77324646 None:intergenic
ACCTCTGGTGCAGTATAGCC+TGG 0.423907 3_3:-77324422 None:intergenic
GCACTCCAAATGTCTGCTTC+AGG 0.426410 3_3:-77324464 None:intergenic
ATGGACCTGAAGCAGACATT+TGG 0.437988 3_3:+77324459 Msa0474410:CDS
GAAACATTTAGTCAAGTTGT+TGG 0.446071 3_3:+77324395 Msa0474410:CDS
AGTCAAGTTGTTGGAACCCC+AGG 0.470655 3_3:+77324404 Msa0474410:CDS
GAGGTCTTGAACGGGCATTA+TGG 0.493285 3_3:+77324440 Msa0474410:CDS
TTGAAAGGAATCCGGAGAAA+AGG 0.507068 3_3:+77324751 Msa0474410:CDS
TTGGCACCGTCTGAAATTGA+AGG 0.509194 3_3:-77324708 None:intergenic
TAATGCCCGTTCAAGACCTC+TGG 0.511246 3_3:-77324437 None:intergenic
TTCAATTTCAGACGGTGCCA+AGG 0.527095 3_3:+77324710 Msa0474410:CDS
GAAGCAGACATTTGGAGTGC+CGG 0.534755 3_3:+77324467 Msa0474410:CDS
CCTCTGGTGCAGTATAGCCT+GGG 0.536066 3_3:-77324421 None:intergenic
CTGCACCAGAGGTCTTGAAC+GGG 0.536546 3_3:+77324432 Msa0474410:CDS
GATAATTTACCTCCCCAAAA+CGG 0.561948 3_3:-77324524 None:intergenic
CCGTCTGAAATTGAAGGCCA+CGG 0.599134 3_3:-77324702 None:intergenic
CTCTGGTGCAGTATAGCCTG+GGG 0.599484 3_3:-77324420 None:intergenic
TTTGTATTTCTTATTAAGCG+GGG 0.602317 3_3:+77324496 Msa0474410:CDS
ACTGCACCAGAGGTCTTGAA+CGG 0.607574 3_3:+77324431 Msa0474410:CDS
TAACCTACAGAGACCAAGGA+GGG 0.610754 3_3:+77324633 Msa0474410:intron
TTGATTTGCAGTCTAAGCCG+TGG 0.614559 3_3:+77324685 Msa0474410:CDS
CCGTGGCCTTCAATTTCAGA+CGG 0.615060 3_3:+77324702 Msa0474410:CDS
CCCAGGCTATACTGCACCAG+AGG 0.637073 3_3:+77324421 Msa0474410:CDS
TAAGAAATACAAAATAACGC+CGG 0.669744 3_3:-77324486 None:intergenic
AACCTACAGAGACCAAGGAG+GGG 0.676140 3_3:+77324634 Msa0474410:intron
TTGTTGAGGAAATAAATGGA+GGG 0.680532 3_3:+77324202 None:intergenic
AATTTACCTCCCCAAAACGG+TGG 0.693079 3_3:-77324521 None:intergenic
TTAACCTACAGAGACCAAGG+AGG 0.760856 3_3:+77324632 Msa0474410:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGTATATATATCATGAAA+GGG - chr3_3:77324352-77324371 None:intergenic 15.0%
!! TTAAGTATATATATCATGAA+AGG - chr3_3:77324353-77324372 None:intergenic 15.0%
!!! ATTTTGTATTTCTTATTAAG+CGG + chr3_3:77324494-77324513 Msa0474410:CDS 15.0%
!!! TTTTGTATTTCTTATTAAGC+GGG + chr3_3:77324495-77324514 Msa0474410:CDS 20.0%
!!! AAATTATCATTAGTTGATGT+AGG + chr3_3:77324539-77324558 Msa0474410:intron 20.0%
!! AACAAATTGATGTGATTTTC+AGG + chr3_3:77324371-77324390 Msa0474410:intron 25.0%
! TAAGAAATACAAAATAACGC+CGG - chr3_3:77324489-77324508 None:intergenic 25.0%
! TTTGTATTTCTTATTAAGCG+GGG + chr3_3:77324496-77324515 Msa0474410:CDS 25.0%
! AATAAGATACTTGCTCTATA+AGG - chr3_3:77324813-77324832 None:intergenic 25.0%
ATATATCATGAAAGGGCTTA+TGG - chr3_3:77324345-77324364 None:intergenic 30.0%
! GAAACATTTAGTCAAGTTGT+TGG + chr3_3:77324395-77324414 Msa0474410:CDS 30.0%
! ATCAACAAACAGATTTTGCA+AGG + chr3_3:77324657-77324676 Msa0474410:CDS 30.0%
TACTTCGAAAAATGCTTGAA+AGG + chr3_3:77324736-77324755 Msa0474410:CDS 30.0%
AAAAATGCTTGAAAGGAATC+CGG + chr3_3:77324743-77324762 Msa0474410:CDS 30.0%
GATAATTTACCTCCCCAAAA+CGG - chr3_3:77324527-77324546 None:intergenic 35.0%
CAGCACAATATTAGCATTAC+TGG - chr3_3:77324604-77324623 None:intergenic 35.0%
! TTTTTAACCTACAGAGACCA+AGG + chr3_3:77324629-77324648 Msa0474410:intron 35.0%
! ACAAACAGATTTTGCAAGGA+AGG + chr3_3:77324661-77324680 Msa0474410:CDS 35.0%
!! CATTTTTCGAAGTAGATCCT+TGG - chr3_3:77324730-77324749 None:intergenic 35.0%
! GAAAGGGCTTATGGTTGATA+TGG - chr3_3:77324336-77324355 None:intergenic 40.0%
TGAAAACAGTGACATGCCTA+CGG - chr3_3:77324565-77324584 None:intergenic 40.0%
TTGAAAGGAATCCGGAGAAA+AGG + chr3_3:77324751-77324770 Msa0474410:CDS 40.0%
ATGGACCTGAAGCAGACATT+TGG + chr3_3:77324459-77324478 Msa0474410:CDS 45.0%
AATTTACCTCCCCAAAACGG+TGG - chr3_3:77324524-77324543 None:intergenic 45.0%
! CATTAGTTGATGTAGGCCGT+AGG + chr3_3:77324546-77324565 Msa0474410:intron 45.0%
TTAACCTACAGAGACCAAGG+AGG + chr3_3:77324632-77324651 Msa0474410:intron 45.0%
TAACCTACAGAGACCAAGGA+GGG + chr3_3:77324633-77324652 Msa0474410:intron 45.0%
!! TTGATTTGCAGTCTAAGCCG+TGG + chr3_3:77324685-77324704 Msa0474410:CDS 45.0%
! TTGGCACCGTCTGAAATTGA+AGG - chr3_3:77324711-77324730 None:intergenic 45.0%
! TTCAATTTCAGACGGTGCCA+AGG + chr3_3:77324710-77324729 Msa0474410:CDS 45.0%
! TGAGCTGTGAACCTTTTCTC+CGG - chr3_3:77324765-77324784 None:intergenic 45.0%
! AGTCAAGTTGTTGGAACCCC+AGG + chr3_3:77324404-77324423 Msa0474410:CDS 50.0%
ACTGCACCAGAGGTCTTGAA+CGG + chr3_3:77324431-77324450 Msa0474410:CDS 50.0%
TAATGCCCGTTCAAGACCTC+TGG - chr3_3:77324440-77324459 None:intergenic 50.0%
GAGGTCTTGAACGGGCATTA+TGG + chr3_3:77324440-77324459 Msa0474410:CDS 50.0%
GCACTCCAAATGTCTGCTTC+AGG - chr3_3:77324467-77324486 None:intergenic 50.0%
! GAAGCAGACATTTGGAGTGC+CGG + chr3_3:77324467-77324486 Msa0474410:CDS 50.0%
AACCTACAGAGACCAAGGAG+GGG + chr3_3:77324634-77324653 Msa0474410:intron 50.0%
! CTGTTTGTTGATCCCCTCCT+TGG - chr3_3:77324649-77324668 None:intergenic 50.0%
! CCGTCTGAAATTGAAGGCCA+CGG - chr3_3:77324705-77324724 None:intergenic 50.0%
CCGTGGCCTTCAATTTCAGA+CGG + chr3_3:77324702-77324721 Msa0474410:CDS 50.0%
CTCTGGTGCAGTATAGCCTG+GGG - chr3_3:77324423-77324442 None:intergenic 55.0%
CCTCTGGTGCAGTATAGCCT+GGG - chr3_3:77324424-77324443 None:intergenic 55.0%
ACCTCTGGTGCAGTATAGCC+TGG - chr3_3:77324425-77324444 None:intergenic 55.0%
CTGCACCAGAGGTCTTGAAC+GGG + chr3_3:77324432-77324451 Msa0474410:CDS 55.0%
ATCCCCTCCTTGGTCTCTGT+AGG - chr3_3:77324639-77324658 None:intergenic 55.0%
CCCAGGCTATACTGCACCAG+AGG + chr3_3:77324421-77324440 Msa0474410:CDS 60.0%
!! TAAGCGGGGTGCCACCGTTT+TGG + chr3_3:77324510-77324529 Msa0474410:CDS 60.0%
!! AAGCGGGGTGCCACCGTTTT+GGG + chr3_3:77324511-77324530 Msa0474410:CDS 60.0%
!! AGCGGGGTGCCACCGTTTTG+GGG + chr3_3:77324512-77324531 Msa0474410:CDS 65.0%
!!! GGGGTGCCACCGTTTTGGGG+AGG + chr3_3:77324515-77324534 Msa0474410:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 77324217 77324839 77324217 ID=Msa0474410;Name=Msa0474410
chr3_3 mRNA 77324217 77324839 77324217 ID=Msa0474410-mRNA-1;Parent=Msa0474410;Name=Msa0474410-mRNA-1;_AED=0.49;_eAED=0.49;_QI=0|0|0|0.66|1|1|3|0|115
chr3_3 exon 77324217 77324223 77324217 ID=Msa0474410-mRNA-1:exon:20604;Parent=Msa0474410-mRNA-1
chr3_3 exon 77324393 77324536 77324393 ID=Msa0474410-mRNA-1:exon:20605;Parent=Msa0474410-mRNA-1
chr3_3 exon 77324643 77324839 77324643 ID=Msa0474410-mRNA-1:exon:20606;Parent=Msa0474410-mRNA-1
chr3_3 CDS 77324217 77324223 77324217 ID=Msa0474410-mRNA-1:cds;Parent=Msa0474410-mRNA-1
chr3_3 CDS 77324393 77324536 77324393 ID=Msa0474410-mRNA-1:cds;Parent=Msa0474410-mRNA-1
chr3_3 CDS 77324643 77324839 77324643 ID=Msa0474410-mRNA-1:cds;Parent=Msa0474410-mRNA-1
Gene Sequence

>Msa0474410

ATGGAGGGTGAAACATTTAGTCAAGTTGTTGGAACCCCAGGCTATACTGCACCAGAGGTCTTGAACGGGCATTATGGACCTGAAGCAGACATTTGGAGTGCCGGCGTTATTTTGTATTTCTTATTAAGCGGGGTGCCACCGTTTTGGGGAGAGACCAAGGAGGGGATCAACAAACAGATTTTGCAAGGAAGGCTTGATTTGCAGTCTAAGCCGTGGCCTTCAATTTCAGACGGTGCCAAGGATCTACTTCGAAAAATGCTTGAAAGGAATCCGGAGAAAAGGTTCACAGCTCATCAAGTGCTCAGTAAGTCTAAACTTCCTTATAGAGCAAGTATCTTATTTATATGA
Protein sequence

>Msa0474410

MEGETFSQVVGTPGYTAPEVLNGHYGPEADIWSAGVILYFLLSGVPPFWGETKEGINKQILQGRLDLQSKPWPSISDGAKDLLRKMLERNPEKRFTAHQVLSKSKLPYRASILFI*