AlfalfaGEDB | Msa0474810

M. sativa cultivar zhongmu-4 / Msa0474810

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009909 GO:0010048 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048580 GO:0048831 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0065007 GO:0070013 GO:0097159 GO:1901363 GO:2000026 GO:2000241
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0474810	RHN70095.1	88.650	326	23	2	1	312	7	332	0.0	591

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0474810	sp\|Q8L3W1\|VRN1_ARATH	27.826	345	168	10	10	283	2	336	7.83e-29	116

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0474810	A0A396J148	88.650	326	23	2	1	312	7	332	0.0	591

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TFs/TRs:

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Gene ID	Type	Classification
Msa0474810	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0474810	MtrunA17_Chr3g0131891	88.650	326	23	2	1	312	7	332	0.0	591
Msa0474810	MtrunA17_Chr7g0233561	63.580	324	94	4	1	300	6	329	2.53e-143	407
Msa0474810	MtrunA17_Chr7g0233531	67.972	281	81	3	28	300	1	280	7.12e-129	368
Msa0474810	MtrunA17_Chr7g0233541	63.973	297	83	6	28	300	1	297	1.16e-123	356
Msa0474810	MtrunA17_Chr7g0233521	59.322	295	83	6	28	300	1	280	1.40e-110	322
Msa0474810	MtrunA17_Chr7g0233571	59.361	219	61	6	1	194	1	216	3.81e-77	234
Msa0474810	MtrunA17_Chr8g0390841	33.333	327	168	11	11	301	37	349	1.53e-43	156
Msa0474810	MtrunA17_Chr1g0163071	33.993	303	137	9	28	283	1	287	5.85e-42	147
Msa0474810	MtrunA17_Chr1g0208771	30.476	315	159	11	29	301	13	309	9.30e-39	142
Msa0474810	MtrunA17_Chr3g0103181	31.164	292	161	10	10	282	9	279	3.31e-34	126

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0474810	AT3G18990.2	27.874	348	170	10	10	286	2	339	7.53e-30	117
Msa0474810	AT3G18990.1	27.826	345	168	10	10	283	2	336	7.96e-30	116
Msa0474810	AT4G33280.1	28.615	325	171	13	11	289	18	327	2.05e-17	82.0
Msa0474810	AT4G01580.1	36.538	104	60	1	13	110	30	133	7.86e-16	74.7
Msa0474810	AT1G49475.1	35.185	108	63	2	13	114	34	140	5.79e-15	72.4
Msa0474810	AT3G18960.2	35.577	104	61	1	13	110	30	133	6.97e-15	72.4
Msa0474810	AT3G18960.1	35.577	104	61	1	13	110	30	133	8.04e-15	72.4
Msa0474810	AT3G18960.3	37.500	88	49	1	29	110	9	96	6.20e-12	63.5

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Find 54 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATATGGTTTCAAAAGGGA+TGG	0.524548	3_3:+77867500	Msa0474810:CDS
AAGTGTCAAATGGCAGAGTT+TGG	0.384047	3_3:+77869042	Msa0474810:CDS
AATTTCCAATTTACACCATT+TGG	0.315099	3_3:-77867454	None:intergenic
ACATGTGTAAAGACTCCAAA+TGG	0.500501	3_3:+77867439	Msa0474810:CDS
ACTACTGCTTCCAATTTCAA+AGG	0.542132	3_3:-77867743	None:intergenic
ACTGCTTCCAATTTCAAAGG+AGG	0.612452	3_3:-77867740	None:intergenic
AGAAAGCTTGTGGAGAAATA+TGG	0.315667	3_3:+77867397	Msa0474810:CDS
AGACTCCAAATGGTGTAAAT+TGG	0.312711	3_3:+77867449	Msa0474810:CDS
ATCCTGATTCGGTACTTTGC+AGG	0.347997	3_3:-77869065	None:intergenic
ATGATCTTGAAGAAGTGAAC+TGG	0.487555	3_3:-77866530	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTTGTGACATACTAAAAGT+TGG	-	chr3_3:77866951-77866970	None:intergenic	30.0%
TAGATGGAGTCTATAAACTA+TGG	+	chr3_3:77867148-77867167	Msa0474810:intron	30.0%
AGATGGAGTCTATAAACTAT+GGG	+	chr3_3:77867149-77867168	Msa0474810:intron	30.0%
ATTTGACAACTCGATGAAAT+TGG	+	chr3_3:77867288-77867307	Msa0474810:intron	30.0%
TGGCAAAATATGGTTTCAAA+AGG	+	chr3_3:77867495-77867514	Msa0474810:CDS	30.0%
GGCAAAATATGGTTTCAAAA+GGG	+	chr3_3:77867496-77867515	Msa0474810:CDS	30.0%
AAATATGGTTTCAAAAGGGA+TGG	+	chr3_3:77867500-77867519	Msa0474810:CDS	30.0%
CATATTTAAAAAGCAGGAGA+TGG	-	chr3_3:77867561-77867580	None:intergenic	30.0%
AAAGATGTGCACATGAAAAA+GGG	-	chr3_3:77867593-77867612	None:intergenic	30.0%
CTTTCTGTCAATATGATGTA+CGG	-	chr3_3:77867800-77867819	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	77866493	77869313	77866493	ID=Msa0474810;Name=Msa0474810
chr3_3	mRNA	77866493	77869313	77866493	ID=Msa0474810-mRNA-1;Parent=Msa0474810;Name=Msa0474810-mRNA-1;_AED=0.11;_eAED=0.11;_QI=10\|0\|0\|0.8\|0.75\|0.6\|5\|0\|312
chr3_3	exon	77866493	77866583	77866493	ID=Msa0474810-mRNA-1:exon:20873;Parent=Msa0474810-mRNA-1
chr3_3	exon	77867388	77867600	77867388	ID=Msa0474810-mRNA-1:exon:20874;Parent=Msa0474810-mRNA-1
chr3_3	exon	77867619	77867826	77867619	ID=Msa0474810-mRNA-1:exon:20875;Parent=Msa0474810-mRNA-1
chr3_3	exon	77868505	77868599	77868505	ID=Msa0474810-mRNA-1:exon:20876;Parent=Msa0474810-mRNA-1
chr3_3	exon	77868975	77869313	77868975	ID=Msa0474810-mRNA-1:exon:20877;Parent=Msa0474810-mRNA-1
chr3_3	five_prime_UTR	77866493	77866502	77866493	ID=Msa0474810-mRNA-1:five_prime_utr;Parent=Msa0474810-mRNA-1
chr3_3	CDS	77866503	77866583	77866503	ID=Msa0474810-mRNA-1:cds;Parent=Msa0474810-mRNA-1
chr3_3	CDS	77867388	77867600	77867388	ID=Msa0474810-mRNA-1:cds;Parent=Msa0474810-mRNA-1
chr3_3	CDS	77867619	77867826	77867619	ID=Msa0474810-mRNA-1:cds;Parent=Msa0474810-mRNA-1
chr3_3	CDS	77868505	77868599	77868505	ID=Msa0474810-mRNA-1:cds;Parent=Msa0474810-mRNA-1
chr3_3	CDS	77868975	77869313	77868975	ID=Msa0474810-mRNA-1:cds;Parent=Msa0474810-mRNA-1

Gene Sequence

>Msa0474810

ATGGCTAACGAAATTCTTCAAGTGAAGCCAGTTCACTTCTTCAAGATCATACTTACTCAAAACCTTCATCAAGGAAAGCTTATGATGCCAAGAAAGCTTGTGGAGAAATATGGAGAATGCTTACCGAAAACTACATGTGTAAAGACTCCAAATGGTGTAAATTGGAAATTAAATTTGGTAAAAAGTGATGGCAAAATATGGTTTCAAAAGGGATGGAAAGAATTTGCAGAGTATCATTCTCTAGCTCATGGCCATCTCCTGCTTTTTAAATATGAAAGAACTTCCCTTTTTCATAGTGCCTTGGAGATAAATAGCCCTTTGACAAGAGTTGAAGCTAAAAAGGTCTTCAACTGTCAAGGAAAAAAACCTTCAAATAATGAAGATTGTAGAGCAAGTCAAAAGAGAAAAGCTAACTCCTCCTTTGAAATTGGAAGCAGTAGTTGTGTTAATGTTCGGAAATTTCAAAAGGCAGCCGTACATCATATTGACAGAAAGGGAAAAGTTACAACACTTGAAAGAGCAAAATCTTTCAAAACATGTAATCCATCCTTTGTTGTTGTCATGGGTGCATCATACGTTGAGCACCATTTTCTTCTGACTATACCGTCTATGTTCGGTAAGAGACACTTTAATTTGAATAAGAAGCGAGGAGATATTCATTTTCAAGTGTCAAATGGCAGAGTTTGGCCTGCAAAGTACCGAATCAGGATGTCCCACACAGGACTAAGATTTGAACTCTCAGGTGGATGGAAGACATTCGCAGAAGACAATAACTTGAAAGTTGGTGATGCTTGCAACTTTGAGCTCATTCTTAGTACTAATATGACTTTCCAAGTTCATATATTCAGGGAGACAGATAAAGACAACGCAGATTGTTTAACATATCAAAGTAAGATTAATTGGTGTTTAATTTTCTTTTCAAATCTTTTTCTCATA

Protein sequence

>Msa0474810

MANEILQVKPVHFFKIILTQNLHQGKLMMPRKLVEKYGECLPKTTCVKTPNGVNWKLNLVKSDGKIWFQKGWKEFAEYHSLAHGHLLLFKYERTSLFHSALEINSPLTRVEAKKVFNCQGKKPSNNEDCRASQKRKANSSFEIGSSSCVNVRKFQKAAVHHIDRKGKVTTLERAKSFKTCNPSFVVVMGASYVEHHFLLTIPSMFGKRHFNLNKKRGDIHFQVSNGRVWPAKYRIRMSHTGLRFELSGGWKTFAEDNNLKVGDACNFELILSTNMTFQVHIFRETDKDNADCLTYQSKINWCLIFFSNLFLI