AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0475860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0475860 MtrunA17_Chr3g0117121 100.000 186 0 0 1 186 1 186 3.84e-138 383
Msa0475860 MtrunA17_Chr4g0058651 75.000 176 34 4 7 177 8 178 2.84e-86 252
Msa0475860 MtrunA17_Chr7g0227511 60.526 190 56 3 1 189 1 172 1.33e-71 214
Msa0475860 MtrunA17_Chr6g0449641 64.151 159 30 2 1 159 1 132 8.99e-66 198
Msa0475860 MtrunA17_Chr6g0449651 78.261 115 25 0 1 115 58 172 8.41e-64 195
Msa0475860 MtrunA17_Chr8g0353981 75.221 113 25 1 1 113 1 110 3.32e-52 167
Msa0475860 MtrunA17_Chr5g0402611 55.556 153 58 1 4 156 2 144 1.68e-51 167
Msa0475860 MtrunA17_Chr5g0437571 71.154 104 30 0 8 111 4 107 2.55e-50 160
Msa0475860 MtrunA17_Chr2g0311601 56.934 137 59 0 9 145 12 148 3.51e-50 159
Msa0475860 MtrunA17_Chr3g0115431 54.140 157 68 2 4 157 3 158 7.10e-48 155
Msa0475860 MtrunA17_Chr4g0043421 65.714 105 36 0 9 113 14 118 1.89e-47 153
Msa0475860 MtrunA17_Chr3g0097221 55.245 143 64 0 5 147 2 144 5.60e-47 155
Msa0475860 MtrunA17_Chr5g0437401 53.571 140 54 1 8 136 9 148 7.14e-47 151
Msa0475860 MtrunA17_Chr3g0106101 64.423 104 37 0 8 111 5 108 2.26e-46 150
Msa0475860 MtrunA17_Chr3g0114061 66.346 104 35 0 8 111 6 109 6.57e-46 148
Msa0475860 MtrunA17_Chr5g0438091 51.408 142 60 2 8 140 37 178 5.63e-44 144
Msa0475860 MtrunA17_Chr3g0129071 60.550 109 43 0 9 117 46 154 1.44e-43 145
Msa0475860 MtrunA17_Chr3g0113891 65.625 96 33 0 8 103 12 107 1.65e-42 140
Msa0475860 MtrunA17_Chr4g0030001 55.455 110 48 1 9 117 23 132 7.93e-40 134
Msa0475860 MtrunA17_Chr1g0200201 58.252 103 43 0 11 113 73 175 2.45e-39 134
Msa0475860 MtrunA17_Chr5g0432451 57.692 130 45 2 9 128 42 171 3.26e-39 134
Msa0475860 MtrunA17_Chr7g0260971 57.798 109 45 1 3 110 7 115 1.80e-38 131
Msa0475860 MtrunA17_Chr8g0351701 57.143 105 44 1 10 113 23 127 1.70e-37 129
Msa0475860 MtrunA17_Chr8g0351721 56.410 117 50 1 10 125 18 134 6.47e-37 127
Msa0475860 MtrunA17_Chr1g0184281 54.701 117 47 2 1 111 1 117 5.70e-36 124
Msa0475860 MtrunA17_Chr7g0245871 61.053 95 36 1 10 103 27 121 9.86e-35 122
Msa0475860 MtrunA17_Chr7g0225111 50.909 110 51 1 9 118 4 110 1.26e-34 118
Msa0475860 MtrunA17_Chr6g0460951 51.818 110 50 2 9 115 39 148 1.27e-33 119
Msa0475860 MtrunA17_Chr5g0435641 51.485 101 48 1 5 104 2 102 2.42e-32 115
Msa0475860 MtrunA17_Chr7g0261031 57.292 96 40 1 9 103 7 102 4.06e-32 115
Msa0475860 MtrunA17_Chr8g0374601 46.789 109 58 0 10 118 26 134 6.57e-32 116
Msa0475860 MtrunA17_Chr1g0184271 54.737 95 42 1 10 103 12 106 9.93e-32 114
Msa0475860 MtrunA17_Chr5g0444351 44.628 121 65 1 11 129 6 126 9.82e-31 109
Msa0475860 MtrunA17_Chr8g0374591 47.115 104 55 0 10 113 8 111 1.34e-30 108
Msa0475860 MtrunA17_Chr7g0245861 47.458 118 59 2 10 124 12 129 8.44e-30 107
Msa0475860 MtrunA17_Chr4g0059901 48.000 100 52 0 11 110 11 110 2.19e-29 109
Msa0475860 MtrunA17_Chr8g0374581 46.364 110 58 1 10 118 8 117 3.86e-28 105
Msa0475860 MtrunA17_Chr6g0452661 45.918 98 52 1 8 104 7 104 5.13e-28 105
Msa0475860 MtrunA17_Chr8g0342351 43.689 103 58 0 11 113 13 115 1.90e-26 101
Msa0475860 MtrunA17_Chr6g0460941 48.315 89 45 1 10 97 7 95 4.68e-26 95.9
Msa0475860 MtrunA17_Chr6g0452671 35.252 139 85 2 11 145 12 149 2.80e-25 97.1
Msa0475860 MtrunA17_Chr6g0452691 39.370 127 72 2 8 129 7 133 3.44e-24 93.6
Msa0475860 MtrunA17_Chr4g0055441 41.053 95 55 1 11 104 3 97 2.15e-23 90.9
Msa0475860 MtrunA17_Chr2g0330201 43.011 93 53 0 11 103 20 112 3.60e-23 93.6
Msa0475860 MtrunA17_Chr4g0040831 30.488 164 103 3 1 161 6 161 1.95e-22 89.7
Msa0475860 MtrunA17_Chr5g0439111 39.423 104 62 1 8 110 7 110 4.40e-20 84.3
Msa0475860 MtrunA17_Chr2g0326041 60.784 51 20 0 9 59 32 82 9.14e-19 77.0
Msa0475860 MtrunA17_Chr4g0046201 29.697 165 99 4 9 164 5 161 2.64e-18 80.1
Msa0475860 MtrunA17_Chr2g0326051 55.932 59 26 0 55 113 5 63 3.04e-16 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0475860 AT5G63090.4 61.929 197 44 5 1 179 1 184 2.55e-73 219
Msa0475860 AT5G63090.3 61.929 197 44 5 1 179 1 184 2.55e-73 219
Msa0475860 AT5G63090.2 61.929 197 44 5 1 179 1 184 2.55e-73 219
Msa0475860 AT5G63090.1 61.929 197 44 5 1 179 1 184 2.55e-73 219
Msa0475860 AT3G27650.1 79.279 111 23 0 4 114 33 143 3.80e-63 192
Msa0475860 AT1G65620.1 72.973 111 30 0 3 113 2 112 4.06e-51 163
Msa0475860 AT1G65620.4 72.973 111 30 0 3 113 2 112 4.06e-51 163
Msa0475860 AT1G65620.3 72.973 111 30 0 3 113 2 112 4.06e-51 163
Msa0475860 AT1G65620.5 72.973 111 30 0 3 113 2 112 4.06e-51 163
Msa0475860 AT1G65620.2 72.973 111 30 0 3 113 2 112 4.06e-51 163
Msa0475860 AT5G66870.1 62.791 129 41 2 1 129 1 122 2.33e-50 165
Msa0475860 AT2G23660.2 60.504 119 43 1 8 126 3 117 6.72e-49 161
Msa0475860 AT2G23660.3 60.504 119 43 1 8 126 3 117 6.72e-49 161
Msa0475860 AT2G23660.1 60.504 119 43 1 8 126 3 117 6.72e-49 161
Msa0475860 AT2G30130.1 69.231 104 32 0 8 111 6 109 7.56e-49 157
Msa0475860 AT2G30340.2 57.759 116 49 0 9 124 52 167 3.73e-44 147
Msa0475860 AT2G30340.1 57.759 116 49 0 9 124 51 166 3.83e-44 147
Msa0475860 AT1G31320.1 72.727 88 24 0 9 96 12 99 1.08e-43 143
Msa0475860 AT1G07900.1 58.333 108 45 0 9 116 32 139 2.37e-43 143
Msa0475860 AT1G16530.1 62.264 106 39 1 9 113 13 118 1.04e-42 140
Msa0475860 AT2G28500.1 60.194 103 41 0 9 111 54 156 2.22e-41 139
Msa0475860 AT3G11090.1 54.167 120 52 2 8 127 9 125 1.14e-39 133
Msa0475860 AT3G26660.1 56.000 100 44 0 11 110 6 105 2.25e-38 128
Msa0475860 AT2G40470.2 53.676 136 50 2 9 139 19 146 2.62e-38 130
Msa0475860 AT3G26620.1 56.000 100 44 0 11 110 6 105 2.64e-38 128
Msa0475860 AT2G40470.1 53.676 136 50 2 9 139 44 171 6.13e-38 130
Msa0475860 AT3G03760.1 55.769 104 45 1 9 111 50 153 2.84e-36 127
Msa0475860 AT2G45420.1 59.596 99 39 1 13 110 40 138 3.08e-35 124
Msa0475860 AT4G00220.1 59.596 99 39 1 13 110 20 118 4.17e-35 123
Msa0475860 AT5G06080.1 42.405 158 87 3 5 161 2 156 4.11e-34 119
Msa0475860 AT2G42430.1 52.294 109 51 1 3 110 8 116 1.02e-33 120
Msa0475860 AT4G00210.1 49.600 125 54 2 3 126 4 120 1.78e-33 119
Msa0475860 AT2G45410.1 55.085 118 47 3 13 126 19 134 2.27e-33 117
Msa0475860 AT2G31310.1 55.208 96 42 1 9 103 6 101 9.09e-33 116
Msa0475860 AT3G58190.1 55.208 96 42 1 10 104 11 106 1.70e-32 116
Msa0475860 AT2G42440.1 52.577 97 45 1 9 104 6 102 8.21e-32 115
Msa0475860 AT5G35900.1 46.610 118 63 0 11 128 6 123 2.63e-31 113
Msa0475860 AT3G50510.1 44.915 118 62 1 9 126 11 125 4.83e-30 109
Msa0475860 AT3G50510.2 44.915 118 62 1 9 126 11 125 4.83e-30 109
Msa0475860 AT3G13850.1 44.660 103 57 0 11 113 37 139 1.32e-28 107
Msa0475860 AT3G47870.1 42.000 100 58 0 11 110 37 136 9.64e-25 98.6
Msa0475860 AT1G72980.1 44.792 96 53 0 9 104 12 107 6.83e-23 91.7
Msa0475860 AT4G22700.1 37.984 129 76 3 11 136 6 133 1.98e-21 87.0
Msa0475860 AT1G06280.1 42.500 80 46 0 8 87 22 101 5.72e-18 78.2
Msa0475860 AT2G19820.1 36.667 90 56 1 9 97 11 100 4.43e-15 68.9
Msa0475860 AT5G15060.1 40.260 77 44 1 7 81 14 90 7.29e-15 68.6
Msa0475860 AT3G27940.3 39.394 66 40 0 10 75 5 70 2.32e-11 59.7
Msa0475860 AT3G27940.1 39.394 66 40 0 10 75 5 70 2.32e-11 59.7
Msa0475860 AT3G27940.2 39.394 66 40 0 10 75 5 70 2.32e-11 59.7

Find 60 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTAGGGTTTCCTCTTTA+AGG 0.073857 3_3:-79396409 Msa0475860:five_prime_UTR
GCATATCTCTCTTCCAATTA+GGG 0.237578 3_3:-79396423 Msa0475860:five_prime_UTR
TGATCAGGAGAATTACTAAA+AGG 0.247150 3_3:+79396277 None:intergenic
TGCATATCTCTCTTCCAATT+AGG 0.250082 3_3:-79396424 Msa0475860:five_prime_UTR
CTTAAAGAGGAAACCCTAAT+TGG 0.304175 3_3:+79396410 None:intergenic
AATTAGGGTTTCCTCTTTAA+GGG 0.304187 3_3:-79396408 Msa0475860:intron
GCCAACGTTCACAAGATCTT+TGG 0.310163 3_3:-79395077 Msa0475860:CDS
TCTTCCTCCTCTTGTTGTTC+CGG 0.318724 3_3:-79394817 Msa0475860:CDS
AGAGGTGGAAGAGAAGAATT+AGG 0.321024 3_3:+79394837 None:intergenic
AGACAGTTTAGTTCAAGATT+TGG 0.321989 3_3:-79394774 Msa0475860:CDS
GGTTCTTCTGGTGGAAAGTA+TGG 0.385993 3_3:+79395110 None:intergenic
TTTGGTAATGGAAATGTTGA+TGG 0.387117 3_3:-79394756 Msa0475860:CDS
AATGGAAATGTTGATGGAAA+TGG 0.392662 3_3:-79394750 Msa0475860:CDS
ATATGTAGGTTTCTTTGATC+AGG 0.407725 3_3:+79396262 None:intergenic
GGTGTAGGAGGAAGAGGAGG+AGG 0.416115 3_3:-79394660 Msa0475860:CDS
AGTGGAGGTGTAGGAGGAAG+AGG 0.435749 3_3:-79394666 Msa0475860:CDS
TTGAAGCTTATATTATTTAG+TGG 0.439849 3_3:+79396486 None:intergenic
GATATAAGTGGAGGTGTAGG+AGG 0.445011 3_3:-79394672 Msa0475860:CDS
TTTAGTTCAAGATTTGGTAA+TGG 0.446442 3_3:-79394768 Msa0475860:CDS
CGTGTGAGAGATCCTGTTTA+TGG 0.449251 3_3:-79394966 Msa0475860:CDS
AATTCACTGCATCTTCTCTC+TGG 0.451599 3_3:+79395012 None:intergenic
TTTACCTGTTTGTGATATGT+AGG 0.459990 3_3:+79396248 None:intergenic
TTAATCCATGGAATAGCATA+AGG 0.469520 3_3:+79394711 None:intergenic
GATCAGGAGAATTACTAAAA+GGG 0.471805 3_3:+79396278 None:intergenic
GCACCAACACAACCATAAAC+AGG 0.472967 3_3:+79394954 None:intergenic
GAAACCTACATATCACAAAC+AGG 0.481296 3_3:-79396252 Msa0475860:intron
GAGGATATAAGTGGAGGTGT+AGG 0.488241 3_3:-79394675 Msa0475860:CDS
CTTCGCCGAAAATGCATGCC+AGG 0.496124 3_3:-79395146 Msa0475860:CDS
CTGCATCTTCTCTCTGGTGA+GGG 0.502538 3_3:+79395018 None:intergenic
TATTATTTAGTGGCAACAAA+AGG 0.503391 3_3:+79396496 None:intergenic
ACAAGTTCAGAGGCTTCAAA+AGG 0.506762 3_3:-79394913 Msa0475860:CDS
GCCTCTGAACTTGTCTCTGA+AGG 0.518925 3_3:+79394922 None:intergenic
GATCCTGTTTATGGTTGTGT+TGG 0.519160 3_3:-79394957 Msa0475860:CDS
GGAGGTGTAGGAGGAAGAGG+AGG 0.524745 3_3:-79394663 Msa0475860:CDS
AAAAGATAGAGAGATATAGA+GGG 0.528555 3_3:+79396463 None:intergenic
GGAACAACAAGAGGAGGAAG+AGG 0.530679 3_3:+79394819 None:intergenic
ACCTCTTCTGAGGATATAAG+TGG 0.541161 3_3:-79394684 Msa0475860:CDS
CATCTTCTCTCTGGTGAGGG+AGG 0.545055 3_3:+79395021 None:intergenic
AGGAATTTGCAGGCTGCACA+TGG 0.545835 3_3:+79395167 None:intergenic
ACTGCATCTTCTCTCTGGTG+AGG 0.556115 3_3:+79395017 None:intergenic
TGAGATTGATGAAACGAAGC+CGG 0.556305 3_3:+79394798 None:intergenic
TCCACTTATATCCTCAGAAG+AGG 0.570244 3_3:+79394683 None:intergenic
TTGGAAGAGAGATATGCAAA+GGG 0.570677 3_3:+79396429 None:intergenic
ACAACAAGAGGAGGAAGAGG+TGG 0.575947 3_3:+79394822 None:intergenic
TCCTTCAGAGACAAGTTCAG+AGG 0.577279 3_3:-79394923 Msa0475860:CDS
GATTAATGATACCTCTTCTG+AGG 0.582743 3_3:-79394694 Msa0475860:CDS
AACGAAGCCGGAACAACAAG+AGG 0.585009 3_3:+79394810 None:intergenic
TATGGTGCAAAGATGCAGCC+TGG 0.588900 3_3:+79395128 None:intergenic
TTCCCTTGCTTATGAAGCCG+AGG 0.588970 3_3:-79394991 Msa0475860:CDS
GCCAAAGATCTTGTGAACGT+TGG 0.589226 3_3:+79395076 None:intergenic
AGGATCTCTCACACGTGCCT+CGG 0.589439 3_3:+79394974 None:intergenic
GAAGAGGTATCATTAATCCA+TGG 0.590052 3_3:+79394699 None:intergenic
GTGCCTCGGCTTCATAAGCA+AGG 0.591344 3_3:+79394988 None:intergenic
ATTGGAAGAGAGATATGCAA+AGG 0.593357 3_3:+79396428 None:intergenic
GGAGCACTATACCCTTAAAG+AGG 0.596441 3_3:+79396397 None:intergenic
GAAGCCGGAACAACAAGAGG+AGG 0.613964 3_3:+79394813 None:intergenic
TTGTCTCTGAAGGAAAGAGA+TGG 0.626573 3_3:+79394932 None:intergenic
TGCCTCGGCTTCATAAGCAA+GGG 0.648971 3_3:+79394989 None:intergenic
GGTGAGGGAGGAGTTCGTTG+AGG 0.665155 3_3:+79395033 None:intergenic
TCTTCTGAGGATATAAGTGG+AGG 0.670110 3_3:-79394681 Msa0475860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATAAAAGCATATAAATT+GGG + chr3_3:79395061-79395080 None:intergenic 10.0%
!!! TAAAATAAAAGCATATAAAT+TGG + chr3_3:79395062-79395081 None:intergenic 10.0%
!!! TATATATATAACTTTTTACT+TGG - chr3_3:79395144-79395163 Msa0475860:CDS 10.0%
!! TAAACATTTATATAATGAAA+AGG + chr3_3:79395677-79395696 None:intergenic 10.0%
!! TTTCATTATATAAATGTTTA+TGG - chr3_3:79395677-79395696 Msa0475860:intron 10.0%
!!! AAAATAAAAGCATATAAATT+GGG + chr3_3:79395061-79395080 None:intergenic 10.0%
!!! TAAAATAAAAGCATATAAAT+TGG + chr3_3:79395062-79395081 None:intergenic 10.0%
!!! TATATATATAACTTTTTACT+TGG - chr3_3:79395144-79395163 Msa0475860:CDS 10.0%
!! TAAACATTTATATAATGAAA+AGG + chr3_3:79395677-79395696 None:intergenic 10.0%
!! TTTCATTATATAAATGTTTA+TGG - chr3_3:79395677-79395696 Msa0475860:intron 10.0%
!! TTGAAGCTTATATTATTTAG+TGG + chr3_3:79394661-79394680 None:intergenic 20.0%
!!! CTATTTTTTTTTTCTTGTGA+AGG - chr3_3:79394815-79394834 Msa0475860:CDS 20.0%
!!! TTTTGAAAAGACACTATTTA+GGG - chr3_3:79395030-79395049 Msa0475860:CDS 20.0%
!! AACCAATAAACTAAATCTTA+AGG - chr3_3:79395254-79395273 Msa0475860:intron 20.0%
!! ACCAATAAACTAAATCTTAA+GGG - chr3_3:79395255-79395274 Msa0475860:intron 20.0%
!! TGATAGAAAAAATCAATGAA+GGG + chr3_3:79395331-79395350 None:intergenic 20.0%
!! ATGATAGAAAAAATCAATGA+AGG + chr3_3:79395332-79395351 None:intergenic 20.0%
!! TGAATCATATAAAGACTTAA+AGG - chr3_3:79395582-79395601 Msa0475860:intron 20.0%
!! AAAGTTATTACAAAACTCAA+TGG + chr3_3:79395811-79395830 None:intergenic 20.0%
!!! TTTTTTTATAACTTTGTAGC+AGG - chr3_3:79395920-79395939 Msa0475860:intron 20.0%
!! TTGAAGCTTATATTATTTAG+TGG + chr3_3:79394661-79394680 None:intergenic 20.0%
!!! CTATTTTTTTTTTCTTGTGA+AGG - chr3_3:79394815-79394834 Msa0475860:CDS 20.0%
!!! TTTTGAAAAGACACTATTTA+GGG - chr3_3:79395030-79395049 Msa0475860:CDS 20.0%
!! AACCAATAAACTAAATCTTA+AGG - chr3_3:79395254-79395273 Msa0475860:intron 20.0%
!! ACCAATAAACTAAATCTTAA+GGG - chr3_3:79395255-79395274 Msa0475860:intron 20.0%
!! TGATAGAAAAAATCAATGAA+GGG + chr3_3:79395331-79395350 None:intergenic 20.0%
!! ATGATAGAAAAAATCAATGA+AGG + chr3_3:79395332-79395351 None:intergenic 20.0%
!! TGAATCATATAAAGACTTAA+AGG - chr3_3:79395582-79395601 Msa0475860:intron 20.0%
!! AAAGTTATTACAAAACTCAA+TGG + chr3_3:79395811-79395830 None:intergenic 20.0%
!!! TTTTTTTATAACTTTGTAGC+AGG - chr3_3:79395920-79395939 Msa0475860:intron 20.0%
! AAAAGATAGAGAGATATAGA+GGG + chr3_3:79394684-79394703 None:intergenic 25.0%
! AAAAAGATAGAGAGATATAG+AGG + chr3_3:79394685-79394704 None:intergenic 25.0%
! TACAAGAACAGATTCAAAAA+AGG - chr3_3:79394838-79394857 Msa0475860:CDS 25.0%
!! CTAAATAGTGTCTTTTCAAA+AGG + chr3_3:79395031-79395050 None:intergenic 25.0%
!!! CTTTTGAAAAGACACTATTT+AGG - chr3_3:79395029-79395048 Msa0475860:CDS 25.0%
! TATATGTATCTTTCTTCTTC+CGG + chr3_3:79395187-79395206 None:intergenic 25.0%
!!! CCTTTTCTTTTTTTGGATTT+TGG + chr3_3:79395281-79395300 None:intergenic 25.0%
! CCAAAATCCAAAAAAAGAAA+AGG - chr3_3:79395278-79395297 Msa0475860:intron 25.0%
!! AACACATCCTTTTCTTTTTT+TGG + chr3_3:79395288-79395307 None:intergenic 25.0%
! TTCTATCATGAATTGACAAA+AGG - chr3_3:79395343-79395362 Msa0475860:intron 25.0%
! ATGATCAAAGAAATATGTGA+AGG - chr3_3:79395715-79395734 Msa0475860:intron 25.0%
! TGATCAAAGAAATATGTGAA+GGG - chr3_3:79395716-79395735 Msa0475860:intron 25.0%
!! TTTAGTTCAAGATTTGGTAA+TGG - chr3_3:79396376-79396395 Msa0475860:intron 25.0%
! AAAAGATAGAGAGATATAGA+GGG + chr3_3:79394684-79394703 None:intergenic 25.0%
! AAAAAGATAGAGAGATATAG+AGG + chr3_3:79394685-79394704 None:intergenic 25.0%
! TACAAGAACAGATTCAAAAA+AGG - chr3_3:79394838-79394857 Msa0475860:CDS 25.0%
!! CTAAATAGTGTCTTTTCAAA+AGG + chr3_3:79395031-79395050 None:intergenic 25.0%
!!! CTTTTGAAAAGACACTATTT+AGG - chr3_3:79395029-79395048 Msa0475860:CDS 25.0%
! TATATGTATCTTTCTTCTTC+CGG + chr3_3:79395187-79395206 None:intergenic 25.0%
!!! CCTTTTCTTTTTTTGGATTT+TGG + chr3_3:79395281-79395300 None:intergenic 25.0%
! CCAAAATCCAAAAAAAGAAA+AGG - chr3_3:79395278-79395297 Msa0475860:intron 25.0%
!! AACACATCCTTTTCTTTTTT+TGG + chr3_3:79395288-79395307 None:intergenic 25.0%
! TTCTATCATGAATTGACAAA+AGG - chr3_3:79395343-79395362 Msa0475860:intron 25.0%
! ATGATCAAAGAAATATGTGA+AGG - chr3_3:79395715-79395734 Msa0475860:intron 25.0%
! TGATCAAAGAAATATGTGAA+GGG - chr3_3:79395716-79395735 Msa0475860:intron 25.0%
!! TTTAGTTCAAGATTTGGTAA+TGG - chr3_3:79396376-79396395 Msa0475860:intron 25.0%
AATTAGGGTTTCCTCTTTAA+GGG - chr3_3:79394736-79394755 Msa0475860:CDS 30.0%
TCACGTAGATTTATTAGATG+AGG + chr3_3:79394771-79394790 None:intergenic 30.0%
! CTTTTACTTCTCATTAGCTA+AGG - chr3_3:79394792-79394811 Msa0475860:CDS 30.0%
GATCAGGAGAATTACTAAAA+GGG + chr3_3:79394869-79394888 None:intergenic 30.0%
TGATCAGGAGAATTACTAAA+AGG + chr3_3:79394870-79394889 None:intergenic 30.0%
ATATGTAGGTTTCTTTGATC+AGG + chr3_3:79394885-79394904 None:intergenic 30.0%
TTTACCTGTTTGTGATATGT+AGG + chr3_3:79394899-79394918 None:intergenic 30.0%
!! TGCTCTTTTGAGTAAAGTAA+CGG - chr3_3:79394985-79395004 Msa0475860:CDS 30.0%
AATAAATCGAACCAAAGATC+TGG + chr3_3:79395106-79395125 None:intergenic 30.0%
! TAACTTTTTACTTGGTCATG+TGG - chr3_3:79395152-79395171 Msa0475860:CDS 30.0%
! GCCCTTAAGATTTAGTTTAT+TGG + chr3_3:79395259-79395278 None:intergenic 30.0%
TACATGAGACTTTACTTTGT+TGG + chr3_3:79395512-79395531 None:intergenic 30.0%
! AGAGTCAATGAAGAGATTTT+AGG - chr3_3:79395554-79395573 Msa0475860:intron 30.0%
!! TCAATGAAGAGATTTTAGGA+GGG - chr3_3:79395558-79395577 Msa0475860:intron 30.0%
! AGACAGTTTAGTTCAAGATT+TGG - chr3_3:79396370-79396389 Msa0475860:intron 30.0%
!! TTTGGTAATGGAAATGTTGA+TGG - chr3_3:79396388-79396407 Msa0475860:intron 30.0%
! AATGGAAATGTTGATGGAAA+TGG - chr3_3:79396394-79396413 Msa0475860:intron 30.0%
TTAATCCATGGAATAGCATA+AGG + chr3_3:79396436-79396455 None:intergenic 30.0%
AATTAGGGTTTCCTCTTTAA+GGG - chr3_3:79394736-79394755 Msa0475860:CDS 30.0%
TCACGTAGATTTATTAGATG+AGG + chr3_3:79394771-79394790 None:intergenic 30.0%
! CTTTTACTTCTCATTAGCTA+AGG - chr3_3:79394792-79394811 Msa0475860:CDS 30.0%
GATCAGGAGAATTACTAAAA+GGG + chr3_3:79394869-79394888 None:intergenic 30.0%
TGATCAGGAGAATTACTAAA+AGG + chr3_3:79394870-79394889 None:intergenic 30.0%
ATATGTAGGTTTCTTTGATC+AGG + chr3_3:79394885-79394904 None:intergenic 30.0%
TTTACCTGTTTGTGATATGT+AGG + chr3_3:79394899-79394918 None:intergenic 30.0%
!! TGCTCTTTTGAGTAAAGTAA+CGG - chr3_3:79394985-79395004 Msa0475860:CDS 30.0%
AATAAATCGAACCAAAGATC+TGG + chr3_3:79395106-79395125 None:intergenic 30.0%
! TAACTTTTTACTTGGTCATG+TGG - chr3_3:79395152-79395171 Msa0475860:CDS 30.0%
! GCCCTTAAGATTTAGTTTAT+TGG + chr3_3:79395259-79395278 None:intergenic 30.0%
TACATGAGACTTTACTTTGT+TGG + chr3_3:79395512-79395531 None:intergenic 30.0%
! AGAGTCAATGAAGAGATTTT+AGG - chr3_3:79395554-79395573 Msa0475860:intron 30.0%
!! TCAATGAAGAGATTTTAGGA+GGG - chr3_3:79395558-79395577 Msa0475860:intron 30.0%
! AGACAGTTTAGTTCAAGATT+TGG - chr3_3:79396370-79396389 Msa0475860:intron 30.0%
!! TTTGGTAATGGAAATGTTGA+TGG - chr3_3:79396388-79396407 Msa0475860:intron 30.0%
! AATGGAAATGTTGATGGAAA+TGG - chr3_3:79396394-79396413 Msa0475860:intron 30.0%
TTAATCCATGGAATAGCATA+AGG + chr3_3:79396436-79396455 None:intergenic 30.0%
TTGGAAGAGAGATATGCAAA+GGG + chr3_3:79394718-79394737 None:intergenic 35.0%
ATTGGAAGAGAGATATGCAA+AGG + chr3_3:79394719-79394738 None:intergenic 35.0%
TGCATATCTCTCTTCCAATT+AGG - chr3_3:79394720-79394739 Msa0475860:CDS 35.0%
GCATATCTCTCTTCCAATTA+GGG - chr3_3:79394721-79394740 Msa0475860:CDS 35.0%
CTTAAAGAGGAAACCCTAAT+TGG + chr3_3:79394737-79394756 None:intergenic 35.0%
CAATTAGGGTTTCCTCTTTA+AGG - chr3_3:79394735-79394754 Msa0475860:CDS 35.0%
GAAACCTACATATCACAAAC+AGG - chr3_3:79394892-79394911 Msa0475860:CDS 35.0%
TTGACAAAAGGAAGAGAGAA+AGG - chr3_3:79395355-79395374 Msa0475860:intron 35.0%
! GTCAATGAAGAGATTTTAGG+AGG - chr3_3:79395557-79395576 Msa0475860:intron 35.0%
ACTAAAAGACACGATGTAGA+AGG - chr3_3:79395851-79395870 Msa0475860:intron 35.0%
CTAAAAGACACGATGTAGAA+GGG - chr3_3:79395852-79395871 Msa0475860:intron 35.0%
CTTTGTAGCAGGAGAAAAAA+TGG - chr3_3:79395931-79395950 Msa0475860:intron 35.0%
!!! AATTTTTGAGGTTCTTCTGG+TGG + chr3_3:79396046-79396065 None:intergenic 35.0%
!!! GCAAATTTTTGAGGTTCTTC+TGG + chr3_3:79396049-79396068 None:intergenic 35.0%
! TGAACGTTGGCAAATTTTTG+AGG + chr3_3:79396058-79396077 None:intergenic 35.0%
! CTTTTCCTTATGCTATTCCA+TGG - chr3_3:79396428-79396447 Msa0475860:five_prime_UTR 35.0%
!! GAAGAGGTATCATTAATCCA+TGG + chr3_3:79396448-79396467 None:intergenic 35.0%
GATTAATGATACCTCTTCTG+AGG - chr3_3:79396450-79396469 Msa0475860:five_prime_UTR 35.0%
TTGGAAGAGAGATATGCAAA+GGG + chr3_3:79394718-79394737 None:intergenic 35.0%
ATTGGAAGAGAGATATGCAA+AGG + chr3_3:79394719-79394738 None:intergenic 35.0%
TGCATATCTCTCTTCCAATT+AGG - chr3_3:79394720-79394739 Msa0475860:CDS 35.0%
GCATATCTCTCTTCCAATTA+GGG - chr3_3:79394721-79394740 Msa0475860:CDS 35.0%
CTTAAAGAGGAAACCCTAAT+TGG + chr3_3:79394737-79394756 None:intergenic 35.0%
CAATTAGGGTTTCCTCTTTA+AGG - chr3_3:79394735-79394754 Msa0475860:CDS 35.0%
GAAACCTACATATCACAAAC+AGG - chr3_3:79394892-79394911 Msa0475860:CDS 35.0%
TTGACAAAAGGAAGAGAGAA+AGG - chr3_3:79395355-79395374 Msa0475860:intron 35.0%
! GTCAATGAAGAGATTTTAGG+AGG - chr3_3:79395557-79395576 Msa0475860:intron 35.0%
ACTAAAAGACACGATGTAGA+AGG - chr3_3:79395851-79395870 Msa0475860:intron 35.0%
CTAAAAGACACGATGTAGAA+GGG - chr3_3:79395852-79395871 Msa0475860:intron 35.0%
CTTTGTAGCAGGAGAAAAAA+TGG - chr3_3:79395931-79395950 Msa0475860:intron 35.0%
!!! AATTTTTGAGGTTCTTCTGG+TGG + chr3_3:79396046-79396065 None:intergenic 35.0%
!!! GCAAATTTTTGAGGTTCTTC+TGG + chr3_3:79396049-79396068 None:intergenic 35.0%
! TGAACGTTGGCAAATTTTTG+AGG + chr3_3:79396058-79396077 None:intergenic 35.0%
! CTTTTCCTTATGCTATTCCA+TGG - chr3_3:79396428-79396447 Msa0475860:five_prime_UTR 35.0%
!! GAAGAGGTATCATTAATCCA+TGG + chr3_3:79396448-79396467 None:intergenic 35.0%
GATTAATGATACCTCTTCTG+AGG - chr3_3:79396450-79396469 Msa0475860:five_prime_UTR 35.0%
TACGTGTGATGTATGTGCTA+GGG - chr3_3:79395530-79395549 Msa0475860:intron 40.0%
AATTCACTGCATCTTCTCTC+TGG + chr3_3:79396135-79396154 None:intergenic 40.0%
GATCCTGTTTATGGTTGTGT+TGG - chr3_3:79396187-79396206 Msa0475860:intron 40.0%
! TTGTCTCTGAAGGAAAGAGA+TGG + chr3_3:79396215-79396234 None:intergenic 40.0%
ACAAGTTCAGAGGCTTCAAA+AGG - chr3_3:79396231-79396250 Msa0475860:intron 40.0%
AGAGGTGGAAGAGAAGAATT+AGG + chr3_3:79396310-79396329 None:intergenic 40.0%
TGAGATTGATGAAACGAAGC+CGG + chr3_3:79396349-79396368 None:intergenic 40.0%
! ACCTCTTCTGAGGATATAAG+TGG - chr3_3:79396460-79396479 Msa0475860:five_prime_UTR 40.0%
TCCACTTATATCCTCAGAAG+AGG + chr3_3:79396464-79396483 None:intergenic 40.0%
! TCTTCTGAGGATATAAGTGG+AGG - chr3_3:79396463-79396482 Msa0475860:five_prime_UTR 40.0%
TACGTGTGATGTATGTGCTA+GGG - chr3_3:79395530-79395549 Msa0475860:intron 40.0%
AATTCACTGCATCTTCTCTC+TGG + chr3_3:79396135-79396154 None:intergenic 40.0%
GATCCTGTTTATGGTTGTGT+TGG - chr3_3:79396187-79396206 Msa0475860:intron 40.0%
! TTGTCTCTGAAGGAAAGAGA+TGG + chr3_3:79396215-79396234 None:intergenic 40.0%
ACAAGTTCAGAGGCTTCAAA+AGG - chr3_3:79396231-79396250 Msa0475860:intron 40.0%
AGAGGTGGAAGAGAAGAATT+AGG + chr3_3:79396310-79396329 None:intergenic 40.0%
TGAGATTGATGAAACGAAGC+CGG + chr3_3:79396349-79396368 None:intergenic 40.0%
! ACCTCTTCTGAGGATATAAG+TGG - chr3_3:79396460-79396479 Msa0475860:five_prime_UTR 40.0%
TCCACTTATATCCTCAGAAG+AGG + chr3_3:79396464-79396483 None:intergenic 40.0%
! TCTTCTGAGGATATAAGTGG+AGG - chr3_3:79396463-79396482 Msa0475860:five_prime_UTR 40.0%
! GGAGCACTATACCCTTAAAG+AGG + chr3_3:79394750-79394769 None:intergenic 45.0%
AACGGTTGCTCGACACAAAA+TGG - chr3_3:79395003-79395022 Msa0475860:CDS 45.0%
GTACGTGTGATGTATGTGCT+AGG - chr3_3:79395529-79395548 Msa0475860:intron 45.0%
! TTTTCGGCGAAGGAATTTGC+AGG + chr3_3:79395990-79396009 None:intergenic 45.0%
GGTTCTTCTGGTGGAAAGTA+TGG + chr3_3:79396037-79396056 None:intergenic 45.0%
GCCAACGTTCACAAGATCTT+TGG - chr3_3:79396067-79396086 Msa0475860:intron 45.0%
GCCAAAGATCTTGTGAACGT+TGG + chr3_3:79396071-79396090 None:intergenic 45.0%
CGTGTGAGAGATCCTGTTTA+TGG - chr3_3:79396178-79396197 Msa0475860:intron 45.0%
GCACCAACACAACCATAAAC+AGG + chr3_3:79396193-79396212 None:intergenic 45.0%
TCCTTCAGAGACAAGTTCAG+AGG - chr3_3:79396221-79396240 Msa0475860:intron 45.0%
!! TCTTCCTCCTCTTGTTGTTC+CGG - chr3_3:79396327-79396346 Msa0475860:intron 45.0%
! GAGGATATAAGTGGAGGTGT+AGG - chr3_3:79396469-79396488 Msa0475860:five_prime_UTR 45.0%
! GATATAAGTGGAGGTGTAGG+AGG - chr3_3:79396472-79396491 Msa0475860:five_prime_UTR 45.0%
! GGAGCACTATACCCTTAAAG+AGG + chr3_3:79394750-79394769 None:intergenic 45.0%
AACGGTTGCTCGACACAAAA+TGG - chr3_3:79395003-79395022 Msa0475860:CDS 45.0%
GTACGTGTGATGTATGTGCT+AGG - chr3_3:79395529-79395548 Msa0475860:intron 45.0%
! TTTTCGGCGAAGGAATTTGC+AGG + chr3_3:79395990-79396009 None:intergenic 45.0%
GGTTCTTCTGGTGGAAAGTA+TGG + chr3_3:79396037-79396056 None:intergenic 45.0%
GCCAACGTTCACAAGATCTT+TGG - chr3_3:79396067-79396086 Msa0475860:intron 45.0%
GCCAAAGATCTTGTGAACGT+TGG + chr3_3:79396071-79396090 None:intergenic 45.0%
CGTGTGAGAGATCCTGTTTA+TGG - chr3_3:79396178-79396197 Msa0475860:intron 45.0%
GCACCAACACAACCATAAAC+AGG + chr3_3:79396193-79396212 None:intergenic 45.0%
TCCTTCAGAGACAAGTTCAG+AGG - chr3_3:79396221-79396240 Msa0475860:intron 45.0%
!! TCTTCCTCCTCTTGTTGTTC+CGG - chr3_3:79396327-79396346 Msa0475860:intron 45.0%
! GAGGATATAAGTGGAGGTGT+AGG - chr3_3:79396469-79396488 Msa0475860:five_prime_UTR 45.0%
! GATATAAGTGGAGGTGTAGG+AGG - chr3_3:79396472-79396491 Msa0475860:five_prime_UTR 45.0%
CTTTGCTGCAGCCAGATCTT+TGG - chr3_3:79395092-79395111 Msa0475860:CDS 50.0%
GGTCATGTGGAGCTGAATTC+CGG - chr3_3:79395165-79395184 Msa0475860:CDS 50.0%
AGGAATTTGCAGGCTGCACA+TGG + chr3_3:79395980-79395999 None:intergenic 50.0%
! TGCAGCCTGGCATGCATTTT+CGG + chr3_3:79396006-79396025 None:intergenic 50.0%
TATGGTGCAAAGATGCAGCC+TGG + chr3_3:79396019-79396038 None:intergenic 50.0%
CTGCATCTTCTCTCTGGTGA+GGG + chr3_3:79396129-79396148 None:intergenic 50.0%
ACTGCATCTTCTCTCTGGTG+AGG + chr3_3:79396130-79396149 None:intergenic 50.0%
TTCCCTTGCTTATGAAGCCG+AGG - chr3_3:79396153-79396172 Msa0475860:intron 50.0%
TGCCTCGGCTTCATAAGCAA+GGG + chr3_3:79396158-79396177 None:intergenic 50.0%
GCCTCTGAACTTGTCTCTGA+AGG + chr3_3:79396225-79396244 None:intergenic 50.0%
ACAACAAGAGGAGGAAGAGG+TGG + chr3_3:79396325-79396344 None:intergenic 50.0%
GGAACAACAAGAGGAGGAAG+AGG + chr3_3:79396328-79396347 None:intergenic 50.0%
AACGAAGCCGGAACAACAAG+AGG + chr3_3:79396337-79396356 None:intergenic 50.0%
CTTTGCTGCAGCCAGATCTT+TGG - chr3_3:79395092-79395111 Msa0475860:CDS 50.0%
GGTCATGTGGAGCTGAATTC+CGG - chr3_3:79395165-79395184 Msa0475860:CDS 50.0%
AGGAATTTGCAGGCTGCACA+TGG + chr3_3:79395980-79395999 None:intergenic 50.0%
! TGCAGCCTGGCATGCATTTT+CGG + chr3_3:79396006-79396025 None:intergenic 50.0%
TATGGTGCAAAGATGCAGCC+TGG + chr3_3:79396019-79396038 None:intergenic 50.0%
CTGCATCTTCTCTCTGGTGA+GGG + chr3_3:79396129-79396148 None:intergenic 50.0%
ACTGCATCTTCTCTCTGGTG+AGG + chr3_3:79396130-79396149 None:intergenic 50.0%
TTCCCTTGCTTATGAAGCCG+AGG - chr3_3:79396153-79396172 Msa0475860:intron 50.0%
TGCCTCGGCTTCATAAGCAA+GGG + chr3_3:79396158-79396177 None:intergenic 50.0%
GCCTCTGAACTTGTCTCTGA+AGG + chr3_3:79396225-79396244 None:intergenic 50.0%
ACAACAAGAGGAGGAAGAGG+TGG + chr3_3:79396325-79396344 None:intergenic 50.0%
GGAACAACAAGAGGAGGAAG+AGG + chr3_3:79396328-79396347 None:intergenic 50.0%
AACGAAGCCGGAACAACAAG+AGG + chr3_3:79396337-79396356 None:intergenic 50.0%
! CTGGCATGCATTTTCGGCGA+AGG + chr3_3:79396000-79396019 None:intergenic 55.0%
CTTCGCCGAAAATGCATGCC+AGG - chr3_3:79395998-79396017 Msa0475860:intron 55.0%
CATCTTCTCTCTGGTGAGGG+AGG + chr3_3:79396126-79396145 None:intergenic 55.0%
GTGCCTCGGCTTCATAAGCA+AGG + chr3_3:79396159-79396178 None:intergenic 55.0%
AGGATCTCTCACACGTGCCT+CGG + chr3_3:79396173-79396192 None:intergenic 55.0%
GAAGCCGGAACAACAAGAGG+AGG + chr3_3:79396334-79396353 None:intergenic 55.0%
! AGTGGAGGTGTAGGAGGAAG+AGG - chr3_3:79396478-79396497 Msa0475860:five_prime_UTR 55.0%
! CTGGCATGCATTTTCGGCGA+AGG + chr3_3:79396000-79396019 None:intergenic 55.0%
CTTCGCCGAAAATGCATGCC+AGG - chr3_3:79395998-79396017 Msa0475860:intron 55.0%
CATCTTCTCTCTGGTGAGGG+AGG + chr3_3:79396126-79396145 None:intergenic 55.0%
GTGCCTCGGCTTCATAAGCA+AGG + chr3_3:79396159-79396178 None:intergenic 55.0%
AGGATCTCTCACACGTGCCT+CGG + chr3_3:79396173-79396192 None:intergenic 55.0%
GAAGCCGGAACAACAAGAGG+AGG + chr3_3:79396334-79396353 None:intergenic 55.0%
! AGTGGAGGTGTAGGAGGAAG+AGG - chr3_3:79396478-79396497 Msa0475860:five_prime_UTR 55.0%
GGTGAGGGAGGAGTTCGTTG+AGG + chr3_3:79396114-79396133 None:intergenic 60.0%
! GGAGGTGTAGGAGGAAGAGG+AGG - chr3_3:79396481-79396500 Msa0475860:five_prime_UTR 60.0%
GGTGTAGGAGGAAGAGGAGG+AGG - chr3_3:79396484-79396503 Msa0475860:five_prime_UTR 60.0%
GGTGAGGGAGGAGTTCGTTG+AGG + chr3_3:79396114-79396133 None:intergenic 60.0%
! GGAGGTGTAGGAGGAAGAGG+AGG - chr3_3:79396481-79396500 Msa0475860:five_prime_UTR 60.0%
GGTGTAGGAGGAAGAGGAGG+AGG - chr3_3:79396484-79396503 Msa0475860:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 79394650 79396516 79394650 ID=Msa0475860;Name=Msa0475860
chr3_3 mRNA 79394650 79396516 79394650 ID=Msa0475860-mRNA-1;Parent=Msa0475860;Name=Msa0475860-mRNA-1;_AED=0.01;_eAED=0.03;_QI=196|0.5|0.66|1|0|0|3|0|189
chr3_3 exon 79394650 79395227 79394650 ID=Msa0475860-mRNA-1:exon:21549;Parent=Msa0475860-mRNA-1
chr3_3 exon 79396253 79396329 79396253 ID=Msa0475860-mRNA-1:exon:21548;Parent=Msa0475860-mRNA-1
chr3_3 exon 79396409 79396516 79396409 ID=Msa0475860-mRNA-1:exon:21547;Parent=Msa0475860-mRNA-1
chr3_3 five_prime_UTR 79396409 79396516 79396409 ID=Msa0475860-mRNA-1:five_prime_utr;Parent=Msa0475860-mRNA-1
chr3_3 five_prime_UTR 79396253 79396329 79396253 ID=Msa0475860-mRNA-1:five_prime_utr;Parent=Msa0475860-mRNA-1
chr3_3 five_prime_UTR 79395217 79395227 79395217 ID=Msa0475860-mRNA-1:five_prime_utr;Parent=Msa0475860-mRNA-1
chr3_3 CDS 79394650 79395216 79394650 ID=Msa0475860-mRNA-1:cds;Parent=Msa0475860-mRNA-1
Gene Sequence

>Msa0475860

ATGGCATCATCAAGCTCATACAATTCACCATGTGCAGCCTGCAAATTCCTTCGCCGAAAATGCATGCCAGGCTGCATCTTTGCACCATACTTTCCACCAGAAGAACCTCAAAAATTTGCCAACGTTCACAAGATCTTTGGCGCAAGCAACGTCACGAAGATCCTCAACGAACTCCTCCCTCACCAGAGAGAAGATGCAGTGAATTCCCTTGCTTATGAAGCCGAGGCACGTGTGAGAGATCCTGTTTATGGTTGTGTTGGTGCCATCTCTTTCCTTCAGAGACAAGTTCAGAGGCTTCAAAAGGAACTTGATTCTGCTAATGCTGATCTTCTTCGCTTTGCTTATAATGAAATCTCTCCTAATTCTTCTCTTCCACCTCTTCCTCCTCTTGTTGTTCCGGCTTCGTTTCATCAATCTCAAAGACAGTTTAGTTCAAGATTTGGTAATGGAAATGTTGATGGAAATGGATTTTACTCTTCTTTTCCTTATGCTATTCCATGGATTAATGATACCTCTTCTGAGGATATAAGTGGAGGTGTAGGAGGAAGAGGAGGAGGTAATAATTTG
Protein sequence

>Msa0475860

MASSSSYNSPCAACKFLRRKCMPGCIFAPYFPPEEPQKFANVHKIFGASNVTKILNELLPHQREDAVNSLAYEAEARVRDPVYGCVGAISFLQRQVQRLQKELDSANADLLRFAYNEISPNSSLPPLPPLVVPASFHQSQRQFSSRFGNGNVDGNGFYSSFPYAIPWINDTSSEDISGGVGGRGGGNNL