AlfalfaGEDB | Msa0478760

M. sativa cultivar zhongmu-4 / Msa0478760

KEGG: -
GO: GO:0001558 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0012505 GO:0016020 GO:0019219 GO:0019222 GO:0030308 GO:0030544 GO:0031072 GO:0031090 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031965 GO:0031967 GO:0031975 GO:0034641 GO:0034645 GO:0040008 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045893 GO:0045926 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0061649 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0140030 GO:0140035 GO:0140036 GO:1901360 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001141
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0478760	XP_013462122.1	93.407	91	6	0	1	91	1	91	1.14e-57	182

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0478760	sp\|Q9SIJ5\|RADL2_ARATH	59.091	88	33	1	7	91	14	101	1.35e-31	109

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0478760	A0A072V2R8	93.407	91	6	0	1	91	1	91	5.46e-58	182

TFs/TRs:

Gene ID	Type	Classification
Msa0478760	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0478760	MtrunA17_Chr3g0141601	93.407	91	6	0	1	91	1	91	1.05e-61	182
Msa0478760	MtrunA17_Chr1g0155181	74.713	87	18	2	1	84	1	86	1.16e-42	134
Msa0478760	MtrunA17_Chr3g0144631	70.588	68	20	0	5	72	14	81	1.97e-32	107
Msa0478760	MtrunA17_Chr3g0081631	57.609	92	34	2	1	91	4	91	2.80e-32	107
Msa0478760	MtrunA17_Chr7g0255841	60.241	83	29	1	9	91	14	92	1.69e-29	100
Msa0478760	MtrunA17_Chr6g0449111	57.143	70	30	0	2	71	5	74	1.13e-26	93.2
Msa0478760	MtrunA17_Chr8g0373111	57.143	70	29	1	4	73	2	70	2.20e-26	92.4
Msa0478760	MtrunA17_Chr8g0373121	57.143	70	29	1	4	73	2	70	2.20e-26	92.4
Msa0478760	MtrunA17_Chr8g0373131	57.143	70	29	1	4	73	2	70	2.97e-26	92.0
Msa0478760	MtrunA17_Chr5g0404101	61.194	67	26	0	3	69	15	81	4.64e-25	89.7
Msa0478760	MtrunA17_Chr5g0404301	63.492	63	22	1	5	67	2	63	9.28e-24	85.5
Msa0478760	MtrunA17_Chr3g0141641	44.444	90	45	1	7	91	3	92	2.01e-23	85.5
Msa0478760	MtrunA17_Chr8g0373101	57.143	70	29	1	4	73	2	70	3.46e-23	84.3
Msa0478760	MtrunA17_Chr5g0429521	46.739	92	38	1	4	84	26	117	4.81e-22	87.0
Msa0478760	MtrunA17_Chr5g0436501	55.385	65	29	0	5	69	25	89	5.36e-21	83.6
Msa0478760	MtrunA17_Chr8g0364741	53.623	69	32	0	1	69	4	72	6.20e-21	80.1
Msa0478760	MtrunA17_Chr1g0162081	56.522	69	30	0	4	72	9	77	1.05e-20	83.2
Msa0478760	MtrunA17_Chr8g0365011	57.576	66	28	0	4	69	5	70	1.81e-20	82.4
Msa0478760	MtrunA17_Chr7g0241761	50.000	78	36	1	5	82	33	107	2.49e-20	82.4
Msa0478760	MtrunA17_Chr3g0104071	55.224	67	30	0	4	70	40	106	2.81e-20	82.4
Msa0478760	MtrunA17_Chr4g0062741	46.667	75	37	2	2	73	3	77	3.62e-17	73.9
Msa0478760	MtrunA17_Chr1g0210471	46.269	67	36	0	5	71	22	88	9.52e-17	72.0
Msa0478760	MtrunA17_Chr1g0194071	49.153	59	29	1	5	62	9	67	8.07e-14	61.2
Msa0478760	MtrunA17_Chr7g0270711	49.206	63	31	1	3	64	7	69	1.55e-13	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0478760	AT2G21650.1	59.091	88	33	1	7	91	14	101	1.37e-32	109
Msa0478760	AT1G75250.1	73.529	68	18	0	2	69	7	74	4.76e-32	108
Msa0478760	AT4G39250.1	58.621	87	34	1	7	91	14	100	6.37e-32	107
Msa0478760	AT1G75250.2	63.736	91	31	2	2	90	7	97	8.02e-32	107
Msa0478760	AT1G19510.1	66.667	66	21	1	10	74	15	80	5.18e-25	90.1
Msa0478760	AT2G18328.1	62.903	62	23	0	10	71	14	75	2.09e-24	87.8
Msa0478760	AT2G38090.1	52.174	69	33	0	4	72	26	94	5.67e-21	84.3
Msa0478760	AT5G58900.1	53.030	66	31	0	4	69	31	96	7.12e-20	81.3
Msa0478760	AT4G36570.1	75.000	44	11	0	7	50	12	55	3.44e-19	74.3
Msa0478760	AT5G05790.2	54.545	66	30	0	4	69	28	93	4.22e-19	79.0
Msa0478760	AT5G05790.1	54.545	66	30	0	4	69	28	93	4.22e-19	79.0
Msa0478760	AT3G11280.2	46.053	76	38	1	7	79	33	108	7.97e-18	75.5
Msa0478760	AT3G11280.1	46.053	76	38	1	7	79	33	108	7.97e-18	75.5
Msa0478760	AT5G01200.1	34.951	103	49	2	5	89	28	130	1.65e-15	69.3
Msa0478760	AT5G08520.1	44.737	76	42	0	1	76	6	81	1.78e-15	69.7
Msa0478760	AT1G49010.1	41.250	80	42	2	5	81	6	83	4.80e-14	65.9
Msa0478760	AT5G04760.1	40.299	67	39	1	3	69	2	67	1.50e-12	60.8
Msa0478760	AT5G23650.1	42.029	69	40	0	1	69	7	75	1.14e-11	59.3

Find 38 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGACACTATCCTTCATTAA+TGG	0.243991	3_3:+83632763	None:intergenic
TAAAATTATTTAATTAAATC+TGG	0.281554	3_3:-83633783	None:intergenic
CCAACATTATTTGTGTAATT+AGG	0.311673	3_3:-83632980	None:intergenic
AGAACCTTGTTGAAGATATT+AGG	0.315510	3_3:+83632930	Msa0478760:CDS
ACATAATGGATCAAGATAAA+AGG	0.337165	3_3:+83633011	Msa0478760:CDS
AGGGACAGAGAGTAATAAAA+AGG	0.360193	3_3:-83633565	None:intergenic
GTCCCTCTCTATGAAGTATC+CGG	0.365923	3_3:+83633582	Msa0478760:three_prime_UTR
TAATGGCTTCAAGTTCGAAT+TGG	0.375169	3_3:+83632780	Msa0478760:exon
TGTTGGTTACAATTACATAA+TGG	0.412132	3_3:+83632997	Msa0478760:CDS
TCTCCACATGTATACTATAT+AGG	0.413310	3_3:-83633650	None:intergenic
CATTATTTGTGTAATTAGGC+AGG	0.435090	3_3:-83632976	None:intergenic
CGTGCATAATCACCCTTTAG+TGG	0.449898	3_3:-83633513	None:intergenic
GGCTCTAAGTCTCCACTAAA+GGG	0.466211	3_3:+83633501	Msa0478760:exon
CACTCCTGATCGTTGGCATA+AGG	0.468590	3_3:+83632856	Msa0478760:CDS
GCCACCTTATGCCAACGATC+AGG	0.483033	3_3:-83632860	None:intergenic
TGAGAATGCGTTGGCAATGT+TGG	0.488680	3_3:+83632826	Msa0478760:CDS
TGTAATTAGGCAGGGGAACA+TGG	0.488710	3_3:-83632967	None:intergenic
CCTAATTACACAAATAATGT+TGG	0.495931	3_3:+83632980	Msa0478760:CDS
TGTATAGAAAATACAATGCC+CGG	0.500439	3_3:-83633601	None:intergenic
AGGCTCTAAGTCTCCACTAA+AGG	0.510302	3_3:+83633500	Msa0478760:CDS
ATTATTTGTGTAATTAGGCA+GGG	0.547965	3_3:-83632975	None:intergenic
GCATAAGGTGGCTAGAGCTG+TGG	0.552998	3_3:+83632871	Msa0478760:CDS
CAAAAGATTTGAGAATGCGT+TGG	0.553213	3_3:+83632817	Msa0478760:CDS
CATAAGGTGGCTAGAGCTGT+GGG	0.555550	3_3:+83632872	Msa0478760:CDS
TCATGCATGATGCAGGATGA+AGG	0.557115	3_3:+83633480	Msa0478760:intron
TCCCTCTCTATGAAGTATCC+GGG	0.572119	3_3:+83633583	Msa0478760:three_prime_UTR
GATATTAGGCAGATCGAGGA+AGG	0.589514	3_3:+83632944	Msa0478760:CDS
TCCTGATCGTTGGCATAAGG+TGG	0.599267	3_3:+83632859	Msa0478760:CDS
TTATTTGTGTAATTAGGCAG+GGG	0.601636	3_3:-83632974	None:intergenic
ACAAGGACACTCCTGATCGT+TGG	0.621807	3_3:+83632849	Msa0478760:CDS
TGCCCGGATACTTCATAGAG+AGG	0.629461	3_3:-83633585	None:intergenic
TGCGTTGGCAATGTTGGACA+AGG	0.631324	3_3:+83632832	Msa0478760:CDS
GCCCGGATACTTCATAGAGA+GGG	0.632827	3_3:-83633584	None:intergenic
TTTCCTATATAGTATACATG+TGG	0.641933	3_3:+83633647	Msa0478760:three_prime_UTR
TGAAGATATTAGGCAGATCG+AGG	0.643314	3_3:+83632940	Msa0478760:CDS
AGGTGGCTAGAGCTGTGGGA+GGG	0.681277	3_3:+83632876	Msa0478760:CDS
AAGGTGGCTAGAGCTGTGGG+AGG	0.685328	3_3:+83632875	Msa0478760:CDS
TCTGCCTAATATCTTCAACA+AGG	0.692316	3_3:-83632934	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATTTATATGTTTATAAGT+AGG	+	chr3_3:83633272-83633291	Msa0478760:intron	10.0%
!!	GATCAAATAAATAATATAAT+TGG	-	chr3_3:83633435-83633454	None:intergenic	10.0%
!!	ATATTTATATGTTTATAAGT+AGG	+	chr3_3:83633272-83633291	Msa0478760:intron	10.0%
!!	GATCAAATAAATAATATAAT+TGG	-	chr3_3:83633435-83633454	None:intergenic	10.0%
!!	TATATTATTTATTTGATCTC+TGG	+	chr3_3:83633437-83633456	Msa0478760:intron	15.0%
!!	GATAAATGATAAAAACATAT+TGG	+	chr3_3:83633700-83633719	Msa0478760:three_prime_UTR	15.0%
!!!	TCAAATAAAATTTTAATCTC+AGG	+	chr3_3:83633744-83633763	Msa0478760:three_prime_UTR	15.0%
!!	TATATTATTTATTTGATCTC+TGG	+	chr3_3:83633437-83633456	Msa0478760:intron	15.0%
!!	GATAAATGATAAAAACATAT+TGG	+	chr3_3:83633700-83633719	Msa0478760:three_prime_UTR	15.0%
!!!	TCAAATAAAATTTTAATCTC+AGG	+	chr3_3:83633744-83633763	Msa0478760:three_prime_UTR	15.0%
!!!	TTTATTCAAGTTTTTTGGTT+TGG	-	chr3_3:83633186-83633205	None:intergenic	20.0%
!!!	TTGGTTTTATTCAAGTTTTT+TGG	-	chr3_3:83633191-83633210	None:intergenic	20.0%
!!!	ATTGCTGTTTTAATTGTTTT+TGG	-	chr3_3:83633210-83633229	None:intergenic	20.0%
!!	GATATTCTTAAAGTTAAGAT+TGG	-	chr3_3:83633342-83633361	None:intergenic	20.0%
!!!	ATTTTATTTGACAGAACTAA+CGG	-	chr3_3:83633735-83633754	None:intergenic	20.0%
!!!	TTTATTCAAGTTTTTTGGTT+TGG	-	chr3_3:83633186-83633205	None:intergenic	20.0%
!!!	TTGGTTTTATTCAAGTTTTT+TGG	-	chr3_3:83633191-83633210	None:intergenic	20.0%
!!!	ATTGCTGTTTTAATTGTTTT+TGG	-	chr3_3:83633210-83633229	None:intergenic	20.0%
!!	GATATTCTTAAAGTTAAGAT+TGG	-	chr3_3:83633342-83633361	None:intergenic	20.0%
!!!	ATTTTATTTGACAGAACTAA+CGG	-	chr3_3:83633735-83633754	None:intergenic	20.0%
!	ATTATTTGTGTAATTAGGCA+GGG	-	chr3_3:83632978-83632997	None:intergenic	25.0%
!	CCAACATTATTTGTGTAATT+AGG	-	chr3_3:83632983-83633002	None:intergenic	25.0%
!	CCTAATTACACAAATAATGT+TGG	+	chr3_3:83632980-83632999	Msa0478760:CDS	25.0%
!!	TGTTGGTTACAATTACATAA+TGG	+	chr3_3:83632997-83633016	Msa0478760:CDS	25.0%
!!!	ACATAATGGATCAAGATAAA+AGG	+	chr3_3:83633011-83633030	Msa0478760:CDS	25.0%
!!!	TGATTTGTTGCAGATTAATA+TGG	+	chr3_3:83633102-83633121	Msa0478760:intron	25.0%
!!	TTCATGTGTAACAAACATTT+TGG	-	chr3_3:83633368-83633387	None:intergenic	25.0%
!	TTTCCTATATAGTATACATG+TGG	+	chr3_3:83633647-83633666	Msa0478760:three_prime_UTR	25.0%
!	ATTATTTGTGTAATTAGGCA+GGG	-	chr3_3:83632978-83632997	None:intergenic	25.0%
!	CCAACATTATTTGTGTAATT+AGG	-	chr3_3:83632983-83633002	None:intergenic	25.0%
!	CCTAATTACACAAATAATGT+TGG	+	chr3_3:83632980-83632999	Msa0478760:CDS	25.0%
!!	TGTTGGTTACAATTACATAA+TGG	+	chr3_3:83632997-83633016	Msa0478760:CDS	25.0%
!!!	ACATAATGGATCAAGATAAA+AGG	+	chr3_3:83633011-83633030	Msa0478760:CDS	25.0%
!!!	TGATTTGTTGCAGATTAATA+TGG	+	chr3_3:83633102-83633121	Msa0478760:intron	25.0%
!!	TTCATGTGTAACAAACATTT+TGG	-	chr3_3:83633368-83633387	None:intergenic	25.0%
!	TTTCCTATATAGTATACATG+TGG	+	chr3_3:83633647-83633666	Msa0478760:three_prime_UTR	25.0%
!	AAATCTTTTGTTCTGCTTTG+CGG	-	chr3_3:83632807-83632826	None:intergenic	30.0%
	AGAACCTTGTTGAAGATATT+AGG	+	chr3_3:83632930-83632949	Msa0478760:CDS	30.0%
	TTATTTGTGTAATTAGGCAG+GGG	-	chr3_3:83632977-83632996	None:intergenic	30.0%
	CATTATTTGTGTAATTAGGC+AGG	-	chr3_3:83632979-83632998	None:intergenic	30.0%
!!	CATTGTGTTTGATTGGTTTA+AGG	-	chr3_3:83633128-83633147	None:intergenic	30.0%
	CTAGTTTCATTGTGTTTGAT+TGG	-	chr3_3:83633135-83633154	None:intergenic	30.0%
!	ACTTTACATAACTAGCTTGA+AGG	-	chr3_3:83633310-83633329	None:intergenic	30.0%
	TGCATGTATGAATAATAAGC+AGG	-	chr3_3:83633394-83633413	None:intergenic	30.0%
	TGCTTATTATTCATACATGC+AGG	+	chr3_3:83633393-83633412	Msa0478760:intron	30.0%
	TGTATAGAAAATACAATGCC+CGG	-	chr3_3:83633604-83633623	None:intergenic	30.0%
	TCTCCACATGTATACTATAT+AGG	-	chr3_3:83633653-83633672	None:intergenic	30.0%
!	AAATCTTTTGTTCTGCTTTG+CGG	-	chr3_3:83632807-83632826	None:intergenic	30.0%
	AGAACCTTGTTGAAGATATT+AGG	+	chr3_3:83632930-83632949	Msa0478760:CDS	30.0%
	TTATTTGTGTAATTAGGCAG+GGG	-	chr3_3:83632977-83632996	None:intergenic	30.0%
	CATTATTTGTGTAATTAGGC+AGG	-	chr3_3:83632979-83632998	None:intergenic	30.0%
!!	CATTGTGTTTGATTGGTTTA+AGG	-	chr3_3:83633128-83633147	None:intergenic	30.0%
	CTAGTTTCATTGTGTTTGAT+TGG	-	chr3_3:83633135-83633154	None:intergenic	30.0%
!	ACTTTACATAACTAGCTTGA+AGG	-	chr3_3:83633310-83633329	None:intergenic	30.0%
	TGCATGTATGAATAATAAGC+AGG	-	chr3_3:83633394-83633413	None:intergenic	30.0%
	TGCTTATTATTCATACATGC+AGG	+	chr3_3:83633393-83633412	Msa0478760:intron	30.0%
	TGTATAGAAAATACAATGCC+CGG	-	chr3_3:83633604-83633623	None:intergenic	30.0%
	TCTCCACATGTATACTATAT+AGG	-	chr3_3:83633653-83633672	None:intergenic	30.0%
!	TAATGGCTTCAAGTTCGAAT+TGG	+	chr3_3:83632780-83632799	Msa0478760:exon	35.0%
	CAAAAGATTTGAGAATGCGT+TGG	+	chr3_3:83632817-83632836	Msa0478760:CDS	35.0%
	TCTGCCTAATATCTTCAACA+AGG	-	chr3_3:83632937-83632956	None:intergenic	35.0%
	GTATTTATCATGCATGATGC+AGG	+	chr3_3:83633473-83633492	Msa0478760:intron	35.0%
	AGGGACAGAGAGTAATAAAA+AGG	-	chr3_3:83633568-83633587	None:intergenic	35.0%
!	TAATGGCTTCAAGTTCGAAT+TGG	+	chr3_3:83632780-83632799	Msa0478760:exon	35.0%
	CAAAAGATTTGAGAATGCGT+TGG	+	chr3_3:83632817-83632836	Msa0478760:CDS	35.0%
	TCTGCCTAATATCTTCAACA+AGG	-	chr3_3:83632937-83632956	None:intergenic	35.0%
	GTATTTATCATGCATGATGC+AGG	+	chr3_3:83633473-83633492	Msa0478760:intron	35.0%
	AGGGACAGAGAGTAATAAAA+AGG	-	chr3_3:83633568-83633587	None:intergenic	35.0%
	TGAAGATATTAGGCAGATCG+AGG	+	chr3_3:83632940-83632959	Msa0478760:CDS	40.0%
	TGAAGATATTAGGCAGATCG+AGG	+	chr3_3:83632940-83632959	Msa0478760:CDS	40.0%
!	TGAGAATGCGTTGGCAATGT+TGG	+	chr3_3:83632826-83632845	Msa0478760:CDS	45.0%
	GATATTAGGCAGATCGAGGA+AGG	+	chr3_3:83632944-83632963	Msa0478760:CDS	45.0%
	TGTAATTAGGCAGGGGAACA+TGG	-	chr3_3:83632970-83632989	None:intergenic	45.0%
	TCATGCATGATGCAGGATGA+AGG	+	chr3_3:83633480-83633499	Msa0478760:intron	45.0%
!!	AGGCTCTAAGTCTCCACTAA+AGG	+	chr3_3:83633500-83633519	Msa0478760:CDS	45.0%
!!	GGCTCTAAGTCTCCACTAAA+GGG	+	chr3_3:83633501-83633520	Msa0478760:exon	45.0%
	CGTGCATAATCACCCTTTAG+TGG	-	chr3_3:83633516-83633535	None:intergenic	45.0%
!	GTCCCTCTCTATGAAGTATC+CGG	+	chr3_3:83633582-83633601	Msa0478760:three_prime_UTR	45.0%
!	TCCCTCTCTATGAAGTATCC+GGG	+	chr3_3:83633583-83633602	Msa0478760:three_prime_UTR	45.0%
!	TGAGAATGCGTTGGCAATGT+TGG	+	chr3_3:83632826-83632845	Msa0478760:CDS	45.0%
	GATATTAGGCAGATCGAGGA+AGG	+	chr3_3:83632944-83632963	Msa0478760:CDS	45.0%
	TGTAATTAGGCAGGGGAACA+TGG	-	chr3_3:83632970-83632989	None:intergenic	45.0%
	TCATGCATGATGCAGGATGA+AGG	+	chr3_3:83633480-83633499	Msa0478760:intron	45.0%
!!	AGGCTCTAAGTCTCCACTAA+AGG	+	chr3_3:83633500-83633519	Msa0478760:CDS	45.0%
!!	GGCTCTAAGTCTCCACTAAA+GGG	+	chr3_3:83633501-83633520	Msa0478760:exon	45.0%
	CGTGCATAATCACCCTTTAG+TGG	-	chr3_3:83633516-83633535	None:intergenic	45.0%
!	GTCCCTCTCTATGAAGTATC+CGG	+	chr3_3:83633582-83633601	Msa0478760:three_prime_UTR	45.0%
!	TCCCTCTCTATGAAGTATCC+GGG	+	chr3_3:83633583-83633602	Msa0478760:three_prime_UTR	45.0%
!!	TTATATATATAAATTGAAAT+TGG	-	chr3_3:83633061-83633080	None:intergenic	5.0%
!!	TAAAATTATTTAATTAAATC+TGG	-	chr3_3:83633786-83633805	None:intergenic	5.0%
!!	TTATATATATAAATTGAAAT+TGG	-	chr3_3:83633061-83633080	None:intergenic	5.0%
!!	TAAAATTATTTAATTAAATC+TGG	-	chr3_3:83633786-83633805	None:intergenic	5.0%
!	TGCGTTGGCAATGTTGGACA+AGG	+	chr3_3:83632832-83632851	Msa0478760:CDS	50.0%
	ACAAGGACACTCCTGATCGT+TGG	+	chr3_3:83632849-83632868	Msa0478760:CDS	50.0%
	CACTCCTGATCGTTGGCATA+AGG	+	chr3_3:83632856-83632875	Msa0478760:CDS	50.0%
	TCCTGATCGTTGGCATAAGG+TGG	+	chr3_3:83632859-83632878	Msa0478760:CDS	50.0%
	CATAAGGTGGCTAGAGCTGT+GGG	+	chr3_3:83632872-83632891	Msa0478760:CDS	50.0%
	GCCCGGATACTTCATAGAGA+GGG	-	chr3_3:83633587-83633606	None:intergenic	50.0%
	TGCCCGGATACTTCATAGAG+AGG	-	chr3_3:83633588-83633607	None:intergenic	50.0%
!	TGCGTTGGCAATGTTGGACA+AGG	+	chr3_3:83632832-83632851	Msa0478760:CDS	50.0%
	ACAAGGACACTCCTGATCGT+TGG	+	chr3_3:83632849-83632868	Msa0478760:CDS	50.0%
	CACTCCTGATCGTTGGCATA+AGG	+	chr3_3:83632856-83632875	Msa0478760:CDS	50.0%
	TCCTGATCGTTGGCATAAGG+TGG	+	chr3_3:83632859-83632878	Msa0478760:CDS	50.0%
	CATAAGGTGGCTAGAGCTGT+GGG	+	chr3_3:83632872-83632891	Msa0478760:CDS	50.0%
	GCCCGGATACTTCATAGAGA+GGG	-	chr3_3:83633587-83633606	None:intergenic	50.0%
	TGCCCGGATACTTCATAGAG+AGG	-	chr3_3:83633588-83633607	None:intergenic	50.0%
	GCCACCTTATGCCAACGATC+AGG	-	chr3_3:83632863-83632882	None:intergenic	55.0%
	GCATAAGGTGGCTAGAGCTG+TGG	+	chr3_3:83632871-83632890	Msa0478760:CDS	55.0%
	GCCACCTTATGCCAACGATC+AGG	-	chr3_3:83632863-83632882	None:intergenic	55.0%
	GCATAAGGTGGCTAGAGCTG+TGG	+	chr3_3:83632871-83632890	Msa0478760:CDS	55.0%
	AAGGTGGCTAGAGCTGTGGG+AGG	+	chr3_3:83632875-83632894	Msa0478760:CDS	60.0%
	AGGTGGCTAGAGCTGTGGGA+GGG	+	chr3_3:83632876-83632895	Msa0478760:CDS	60.0%
	AAGGTGGCTAGAGCTGTGGG+AGG	+	chr3_3:83632875-83632894	Msa0478760:CDS	60.0%
	AGGTGGCTAGAGCTGTGGGA+GGG	+	chr3_3:83632876-83632895	Msa0478760:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	83632778	83633820	83632778	ID=Msa0478760;Name=Msa0478760
chr3_3	mRNA	83632778	83633820	83632778	ID=Msa0478760-mRNA-1;Parent=Msa0478760;Name=Msa0478760-mRNA-1;_AED=0.02;_eAED=0.02;_QI=4\|1\|1\|1\|1\|1\|2\|301\|91
chr3_3	exon	83632778	83633032	83632778	ID=Msa0478760-mRNA-1:exon:23529;Parent=Msa0478760-mRNA-1
chr3_3	exon	83633495	83633820	83633495	ID=Msa0478760-mRNA-1:exon:23530;Parent=Msa0478760-mRNA-1
chr3_3	five_prime_UTR	83632778	83632781	83632778	ID=Msa0478760-mRNA-1:five_prime_utr;Parent=Msa0478760-mRNA-1
chr3_3	CDS	83632782	83633032	83632782	ID=Msa0478760-mRNA-1:cds;Parent=Msa0478760-mRNA-1
chr3_3	CDS	83633495	83633519	83633495	ID=Msa0478760-mRNA-1:cds;Parent=Msa0478760-mRNA-1
chr3_3	three_prime_UTR	83633520	83633820	83633520	ID=Msa0478760-mRNA-1:three_prime_utr;Parent=Msa0478760-mRNA-1

Gene Sequence	>Msa0478760 ATGGCTTCAAGTTCGAATTGGACCGCAAAGCAGAACAAAAGATTTGAGAATGCGTTGGCAATGTTGGACAAGGACACTCCTGATCGTTGGCATAAGGTGGCTAGAGCTGTGGGAGGGAAGACTGTTGAAGAAGTGAAAAGACATTATGAGAACCTTGTTGAAGATATTAGGCAGATCGAGGAAGGCCATGTTCCCCTGCCTAATTACACAAATAATGTTGGTTACAATTACATAATGGATCAAGATAAAAGGATGAAGGCTCTAAGTCTCCACTAA
Protein sequence	>Msa0478760 MASSSNWTAKQNKRFENALAMLDKDTPDRWHKVARAVGGKTVEEVKRHYENLVEDIRQIEEGHVPLPNYTNNVGYNYIMDQDKRMKALSLH*