Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478930 | KEH17998.1 | 61.111 | 72 | 28 | 0 | 1 | 72 | 1 | 72 | 2.18e-18 | 85.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478930 | A0A072TMD2 | 61.111 | 72 | 28 | 0 | 1 | 72 | 1 | 72 | 1.04e-18 | 85.1 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0478930 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478930 | MtrunA17_Chr8g0337271 | 61.111 | 72 | 28 | 0 | 1 | 72 | 1 | 72 | 2.00e-22 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGTCATGCATTCAGATTT+TGG | 0.322747 | 3_3:+83782055 | Msa0478930:CDS |
| GCCGATTCCCTTCCCACATA+AGG | 0.384016 | 3_3:+83782133 | Msa0478930:CDS |
| CATGGCACTGATTGTGAATT+CGG | 0.384489 | 3_3:+83781995 | Msa0478930:CDS |
| GCAACAACTTGTCTTTGAAA+TGG | 0.405825 | 3_3:-83782112 | None:intergenic |
| TCACCAAGCTTCTCCTTATG+TGG | 0.415940 | 3_3:-83782146 | None:intergenic |
| TCCTTATGTGGGAAGGGAAT+CGG | 0.429301 | 3_3:-83782134 | None:intergenic |
| TCTATTAAATGATTATCATC+AGG | 0.466595 | 3_3:-83782088 | None:intergenic |
| TGTGTCGACATATGAGAAGA+AGG | 0.509016 | 3_3:+83781972 | Msa0478930:CDS |
| AGCTTCTCCTTATGTGGGAA+GGG | 0.537770 | 3_3:-83782140 | None:intergenic |
| CACCAAGCTTCTCCTTATGT+GGG | 0.561757 | 3_3:-83782145 | None:intergenic |
| CAATGAATGAGAGAAATACA+AGG | 0.575351 | 3_3:+83781933 | None:intergenic |
| AAGCTTCTCCTTATGTGGGA+AGG | 0.575365 | 3_3:-83782141 | None:intergenic |
| CTGAAAGTCAGCATACCTGT+TGG | 0.621934 | 3_3:-83782032 | None:intergenic |
| CGACATATGAGAAGAAGGCA+TGG | 0.648112 | 3_3:+83781977 | Msa0478930:CDS |
| AATGAATGAGAGAAATACAA+GGG | 0.672892 | 3_3:+83781934 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTATTAAATGATTATCATC+AGG | - | chr3_3:83782091-83782110 | None:intergenic | 20.0% |
| !! | TGAATTCGGTCTATATTTTA+CGG | + | chr3_3:83782009-83782028 | Msa0478930:CDS | 25.0% |
| ! | AGTGTCATGCATTCAGATTT+TGG | + | chr3_3:83782055-83782074 | Msa0478930:CDS | 35.0% |
| ! | GTGTCATGCATTCAGATTTT+GGG | + | chr3_3:83782056-83782075 | Msa0478930:CDS | 35.0% |
| ! | TGTCATGCATTCAGATTTTG+GGG | + | chr3_3:83782057-83782076 | Msa0478930:CDS | 35.0% |
| ! | GCAACAACTTGTCTTTGAAA+TGG | - | chr3_3:83782115-83782134 | None:intergenic | 35.0% |
| ! | TGTGTCGACATATGAGAAGA+AGG | + | chr3_3:83781972-83781991 | Msa0478930:CDS | 40.0% |
| !! | CATGGCACTGATTGTGAATT+CGG | + | chr3_3:83781995-83782014 | Msa0478930:CDS | 40.0% |
| ! | GTCTATATTTTACGGCCAAC+AGG | + | chr3_3:83782017-83782036 | Msa0478930:CDS | 40.0% |
| CGACATATGAGAAGAAGGCA+TGG | + | chr3_3:83781977-83781996 | Msa0478930:CDS | 45.0% | |
| CTGAAAGTCAGCATACCTGT+TGG | - | chr3_3:83782035-83782054 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 83781935 | 83782153 | 83781935 | ID=Msa0478930;Name=Msa0478930 |
| chr3_3 | mRNA | 83781935 | 83782153 | 83781935 | ID=Msa0478930-mRNA-1;Parent=Msa0478930;Name=Msa0478930-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|72 |
| chr3_3 | exon | 83781935 | 83782153 | 83781935 | ID=Msa0478930-mRNA-1:exon:23696;Parent=Msa0478930-mRNA-1 |
| chr3_3 | CDS | 83781935 | 83782153 | 83781935 | ID=Msa0478930-mRNA-1:cds;Parent=Msa0478930-mRNA-1 |
| Gene Sequence |
| Protein sequence |
