AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0483930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0483930 MtrunA17_Chr7g0214511 83.946 299 22 5 10 302 9 287 1.52e-169 471
Msa0483930 MtrunA17_Chr1g0174001 60.309 194 58 8 118 302 12 195 1.88e-63 198
Msa0483930 MtrunA17_Chr1g0169521 49.808 261 83 9 60 302 86 316 1.53e-61 197
Msa0483930 MtrunA17_Chr1g0169561 49.624 266 83 12 56 302 83 316 4.21e-60 194
Msa0483930 MtrunA17_Chr1g0193731 47.576 330 81 17 16 302 15 295 2.63e-58 189
Msa0483930 MtrunA17_Chr1g0180581 44.444 288 84 11 15 302 9 220 4.40e-51 167
Msa0483930 MtrunA17_Chr1g0174871 35.907 259 121 11 72 301 42 284 1.83e-22 94.7
Msa0483930 MtrunA17_Chr1g0174861 37.363 182 88 9 93 268 63 224 2.10e-20 88.2
Msa0483930 MtrunA17_Chr1g0180571 78.431 51 11 0 247 297 22 72 3.71e-19 82.0
Msa0483930 MtrunA17_Chr1g0172861 35.055 271 102 15 72 302 42 278 1.54e-16 78.2
Msa0483930 MtrunA17_Chr1g0172961 32.411 253 101 13 72 284 42 264 1.75e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAAGGTGTTACTGAATT+AGG 0.100666 3_4:-782837 Msa0483930:CDS
TGAATCACCGTGGCGCTAAA+AGG 0.215611 3_4:-783085 Msa0483930:CDS
TCGACGCGGCCGGCGTTTCT+TGG 0.252455 3_4:+783211 None:intergenic
TTCTTGGTGTTGTAGAATAT+TGG 0.276502 3_4:+783227 None:intergenic
CTCTTCAATAGCATGATTAG+AGG 0.355993 3_4:+782869 None:intergenic
ATGAAGGTGTTACTGAATTA+GGG 0.357850 3_4:-782836 Msa0483930:CDS
TTCTGTTCTTGGTAATCATA+AGG 0.363019 3_4:-783429 Msa0483930:CDS
ATCGCTCCCTTCAAAATTCC+AGG 0.381362 3_4:-783580 Msa0483930:CDS
CAAAGCATTGAATGGACATA+AGG 0.388435 3_4:-783324 Msa0483930:CDS
TATATCTCGCGGAGGTTATG+AGG 0.390309 3_4:-783129 Msa0483930:CDS
AGTACTTTGAACAGTCAATA+TGG 0.393472 3_4:+783255 None:intergenic
AAAACTTTCTTGCAATATGA+TGG 0.405590 3_4:-783060 Msa0483930:CDS
GCATCATTAGTTGTTGAAGA+TGG 0.412853 3_4:+783500 None:intergenic
ACTTCTAACAAAGCATTGAA+TGG 0.414800 3_4:-783332 Msa0483930:CDS
ATAGCATGATTAGAGGTAGA+AGG 0.440842 3_4:+782876 None:intergenic
GAAGATGGTGGTGCAGTTTG+TGG 0.454649 3_4:+783290 None:intergenic
GAATAGGGTTTGAGTAATCA+AGG 0.456225 3_4:+783540 None:intergenic
AAAGCATTGAATGGACATAA+GGG 0.470952 3_4:-783323 Msa0483930:CDS
TTCTTCTTCTTCGACGCGGC+CGG 0.486419 3_4:+783201 None:intergenic
GAATCACCGTGGCGCTAAAA+GGG 0.488695 3_4:-783084 Msa0483930:CDS
TAATCAAGGAAACGGAAAGA+AGG 0.496330 3_4:+783554 None:intergenic
GTCAATATGGCTCTGTTCAA+AGG 0.497478 3_4:+783268 None:intergenic
TCTTCAACAACTAATGATGC+TGG 0.499426 3_4:-783497 Msa0483930:CDS
GGAGCGATGATAAACATCAT+TGG 0.502169 3_4:+783595 None:intergenic
GAAGAGAAGAAGATTAATGA+AGG 0.510676 3_4:-782852 Msa0483930:CDS
TCAAACATCACAAGATTGCA+AGG 0.513757 3_4:-782901 Msa0483930:CDS
CTCATGTGATGTTTGCAACA+AGG 0.516766 3_4:-783360 Msa0483930:CDS
TTGGTGTTGTAGAATATTGG+CGG 0.527171 3_4:+783230 None:intergenic
TTGAGGTTCAAGATTCCTAA+AGG 0.528982 3_4:-782933 Msa0483930:CDS
AAGATGGTGGTGCAGTTTGT+GGG 0.535375 3_4:+783291 None:intergenic
GAAACGGAAAGAAGGGTACC+TGG 0.540917 3_4:+783562 None:intergenic
TAGCCACAATGAAAAGAAGT+TGG 0.545096 3_4:-782958 Msa0483930:CDS
GGTTTGAGTAATCAAGGAAA+CGG 0.550662 3_4:+783546 None:intergenic
TTTGGTTCATGATCATGAGA+AGG 0.562074 3_4:+783620 None:intergenic
TCATGATCATGAGAAGGAGA+TGG 0.576941 3_4:+783626 None:intergenic
TGGCTCTGTTCAAAGGAAGA+TGG 0.582600 3_4:+783275 None:intergenic
AATCAAGGAAACGGAAAGAA+GGG 0.583538 3_4:+783555 None:intergenic
TCATCTTCAAAATCATCACT+TGG 0.584649 3_4:+782777 None:intergenic
CAAGGTGTGTTTCTAACAAG+TGG 0.594003 3_4:-783008 Msa0483930:CDS
ATGAAAAGAAGTTGGTGTTG+AGG 0.594249 3_4:-782950 Msa0483930:CDS
ACAACGTGAAGAAGATCGTA+TGG 0.595118 3_4:-783463 Msa0483930:CDS
ATATCTCGCGGAGGTTATGA+GGG 0.614101 3_4:-783128 Msa0483930:CDS
ACTTTGTTGCATATATCTCG+CGG 0.675938 3_4:-783140 Msa0483930:CDS
TCTACAACACCAAGAAACGC+CGG 0.683622 3_4:-783220 Msa0483930:CDS
TATCTTCTTCTTCTTCGACG+CGG 0.720402 3_4:+783197 None:intergenic
CTCTGTTCAAAGGAAGATGG+TGG 0.725506 3_4:+783278 None:intergenic
TTGTTGCATATATCTCGCGG+AGG 0.742758 3_4:-783137 Msa0483930:CDS
AATATCGATGTGAATCACCG+TGG 0.797456 3_4:-783095 Msa0483930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCTTATTCTGTTCT+TGG - chr3_4:782980-782999 Msa0483930:CDS 20.0%
!!! TTTTTTTCTTATTCTGTTCT+TGG - chr3_4:782980-782999 Msa0483930:CDS 20.0%
!!! TGATAAACATCATTGGTTTT+TGG + chr3_4:782821-782840 None:intergenic 25.0%
!!! TTTTGAGTTCATTCTGAATA+GGG + chr3_4:782898-782917 None:intergenic 25.0%
!!! TTTTTGAGTTCATTCTGAAT+AGG + chr3_4:782899-782918 None:intergenic 25.0%
! AAAACTTTCTTGCAATATGA+TGG - chr3_4:783360-783379 Msa0483930:CDS 25.0%
!!! TGATAAACATCATTGGTTTT+TGG + chr3_4:782821-782840 None:intergenic 25.0%
!!! TTTTGAGTTCATTCTGAATA+GGG + chr3_4:782898-782917 None:intergenic 25.0%
!!! TTTTTGAGTTCATTCTGAAT+AGG + chr3_4:782899-782918 None:intergenic 25.0%
! AAAACTTTCTTGCAATATGA+TGG - chr3_4:783360-783379 Msa0483930:CDS 25.0%
! TTCTGTTCTTGGTAATCATA+AGG - chr3_4:782991-783010 Msa0483930:CDS 30.0%
!! ACTTCTAACAAAGCATTGAA+TGG - chr3_4:783088-783107 Msa0483930:CDS 30.0%
!! AAAGCATTGAATGGACATAA+GGG - chr3_4:783097-783116 Msa0483930:CDS 30.0%
AGTACTTTGAACAGTCAATA+TGG + chr3_4:783168-783187 None:intergenic 30.0%
!! TTCTTGGTGTTGTAGAATAT+TGG + chr3_4:783196-783215 None:intergenic 30.0%
GTTTGAAAAATCTTGCCTTT+AGG + chr3_4:783505-783524 None:intergenic 30.0%
GAAGAGAAGAAGATTAATGA+AGG - chr3_4:783568-783587 Msa0483930:CDS 30.0%
!! AATGAAGGTGTTACTGAATT+AGG - chr3_4:783583-783602 Msa0483930:CDS 30.0%
!! ATGAAGGTGTTACTGAATTA+GGG - chr3_4:783584-783603 Msa0483930:CDS 30.0%
TCATCTTCAAAATCATCACT+TGG + chr3_4:783646-783665 None:intergenic 30.0%
! TTCTGTTCTTGGTAATCATA+AGG - chr3_4:782991-783010 Msa0483930:CDS 30.0%
!! ACTTCTAACAAAGCATTGAA+TGG - chr3_4:783088-783107 Msa0483930:CDS 30.0%
!! AAAGCATTGAATGGACATAA+GGG - chr3_4:783097-783116 Msa0483930:CDS 30.0%
AGTACTTTGAACAGTCAATA+TGG + chr3_4:783168-783187 None:intergenic 30.0%
!! TTCTTGGTGTTGTAGAATAT+TGG + chr3_4:783196-783215 None:intergenic 30.0%
GTTTGAAAAATCTTGCCTTT+AGG + chr3_4:783505-783524 None:intergenic 30.0%
GAAGAGAAGAAGATTAATGA+AGG - chr3_4:783568-783587 Msa0483930:CDS 30.0%
!! AATGAAGGTGTTACTGAATT+AGG - chr3_4:783583-783602 Msa0483930:CDS 30.0%
!! ATGAAGGTGTTACTGAATTA+GGG - chr3_4:783584-783603 Msa0483930:CDS 30.0%
TCATCTTCAAAATCATCACT+TGG + chr3_4:783646-783665 None:intergenic 30.0%
! TTTGGTTCATGATCATGAGA+AGG + chr3_4:782803-782822 None:intergenic 35.0%
AATCAAGGAAACGGAAAGAA+GGG + chr3_4:782868-782887 None:intergenic 35.0%
TAATCAAGGAAACGGAAAGA+AGG + chr3_4:782869-782888 None:intergenic 35.0%
GGTTTGAGTAATCAAGGAAA+CGG + chr3_4:782877-782896 None:intergenic 35.0%
GAATAGGGTTTGAGTAATCA+AGG + chr3_4:782883-782902 None:intergenic 35.0%
GCATCATTAGTTGTTGAAGA+TGG + chr3_4:782923-782942 None:intergenic 35.0%
TCTTCAACAACTAATGATGC+TGG - chr3_4:782923-782942 Msa0483930:CDS 35.0%
!! CAAAGCATTGAATGGACATA+AGG - chr3_4:783096-783115 Msa0483930:CDS 35.0%
!! TTGGTGTTGTAGAATATTGG+CGG + chr3_4:783193-783212 None:intergenic 35.0%
ACTTTGTTGCATATATCTCG+CGG - chr3_4:783280-783299 Msa0483930:CDS 35.0%
TGATGAGAAGAAGAAGAAGA+AGG - chr3_4:783387-783406 Msa0483930:CDS 35.0%
AAGAAGAAGAAGAAGGAACA+AGG - chr3_4:783394-783413 Msa0483930:CDS 35.0%
TAGCCACAATGAAAAGAAGT+TGG - chr3_4:783462-783481 Msa0483930:CDS 35.0%
! ACACCAACTTCTTTTCATTG+TGG + chr3_4:783468-783487 None:intergenic 35.0%
!! ATGAAAAGAAGTTGGTGTTG+AGG - chr3_4:783470-783489 Msa0483930:CDS 35.0%
TTGAGGTTCAAGATTCCTAA+AGG - chr3_4:783487-783506 Msa0483930:CDS 35.0%
TCAAACATCACAAGATTGCA+AGG - chr3_4:783519-783538 Msa0483930:CDS 35.0%
ATAGCATGATTAGAGGTAGA+AGG + chr3_4:783547-783566 None:intergenic 35.0%
CTCTTCAATAGCATGATTAG+AGG + chr3_4:783554-783573 None:intergenic 35.0%
! TTTGGTTCATGATCATGAGA+AGG + chr3_4:782803-782822 None:intergenic 35.0%
AATCAAGGAAACGGAAAGAA+GGG + chr3_4:782868-782887 None:intergenic 35.0%
TAATCAAGGAAACGGAAAGA+AGG + chr3_4:782869-782888 None:intergenic 35.0%
GGTTTGAGTAATCAAGGAAA+CGG + chr3_4:782877-782896 None:intergenic 35.0%
GAATAGGGTTTGAGTAATCA+AGG + chr3_4:782883-782902 None:intergenic 35.0%
GCATCATTAGTTGTTGAAGA+TGG + chr3_4:782923-782942 None:intergenic 35.0%
TCTTCAACAACTAATGATGC+TGG - chr3_4:782923-782942 Msa0483930:CDS 35.0%
!! CAAAGCATTGAATGGACATA+AGG - chr3_4:783096-783115 Msa0483930:CDS 35.0%
!! TTGGTGTTGTAGAATATTGG+CGG + chr3_4:783193-783212 None:intergenic 35.0%
ACTTTGTTGCATATATCTCG+CGG - chr3_4:783280-783299 Msa0483930:CDS 35.0%
TGATGAGAAGAAGAAGAAGA+AGG - chr3_4:783387-783406 Msa0483930:CDS 35.0%
AAGAAGAAGAAGAAGGAACA+AGG - chr3_4:783394-783413 Msa0483930:CDS 35.0%
TAGCCACAATGAAAAGAAGT+TGG - chr3_4:783462-783481 Msa0483930:CDS 35.0%
! ACACCAACTTCTTTTCATTG+TGG + chr3_4:783468-783487 None:intergenic 35.0%
!! ATGAAAAGAAGTTGGTGTTG+AGG - chr3_4:783470-783489 Msa0483930:CDS 35.0%
TTGAGGTTCAAGATTCCTAA+AGG - chr3_4:783487-783506 Msa0483930:CDS 35.0%
TCAAACATCACAAGATTGCA+AGG - chr3_4:783519-783538 Msa0483930:CDS 35.0%
ATAGCATGATTAGAGGTAGA+AGG + chr3_4:783547-783566 None:intergenic 35.0%
CTCTTCAATAGCATGATTAG+AGG + chr3_4:783554-783573 None:intergenic 35.0%
TCATGATCATGAGAAGGAGA+TGG + chr3_4:782797-782816 None:intergenic 40.0%
GGAGCGATGATAAACATCAT+TGG + chr3_4:782828-782847 None:intergenic 40.0%
!! AGGGTACCTGGAATTTTGAA+GGG + chr3_4:782849-782868 None:intergenic 40.0%
!!! AAGGGTACCTGGAATTTTGA+AGG + chr3_4:782850-782869 None:intergenic 40.0%
ACAACGTGAAGAAGATCGTA+TGG - chr3_4:782957-782976 Msa0483930:CDS 40.0%
CTCATGTGATGTTTGCAACA+AGG - chr3_4:783060-783079 Msa0483930:CDS 40.0%
! GTCAATATGGCTCTGTTCAA+AGG + chr3_4:783155-783174 None:intergenic 40.0%
TATCTTCTTCTTCTTCGACG+CGG + chr3_4:783226-783245 None:intergenic 40.0%
AATATCGATGTGAATCACCG+TGG - chr3_4:783325-783344 Msa0483930:CDS 40.0%
! CAAGGTGTGTTTCTAACAAG+TGG - chr3_4:783412-783431 Msa0483930:CDS 40.0%
TCATGATCATGAGAAGGAGA+TGG + chr3_4:782797-782816 None:intergenic 40.0%
GGAGCGATGATAAACATCAT+TGG + chr3_4:782828-782847 None:intergenic 40.0%
!! AGGGTACCTGGAATTTTGAA+GGG + chr3_4:782849-782868 None:intergenic 40.0%
!!! AAGGGTACCTGGAATTTTGA+AGG + chr3_4:782850-782869 None:intergenic 40.0%
ACAACGTGAAGAAGATCGTA+TGG - chr3_4:782957-782976 Msa0483930:CDS 40.0%
CTCATGTGATGTTTGCAACA+AGG - chr3_4:783060-783079 Msa0483930:CDS 40.0%
! GTCAATATGGCTCTGTTCAA+AGG + chr3_4:783155-783174 None:intergenic 40.0%
TATCTTCTTCTTCTTCGACG+CGG + chr3_4:783226-783245 None:intergenic 40.0%
AATATCGATGTGAATCACCG+TGG - chr3_4:783325-783344 Msa0483930:CDS 40.0%
! CAAGGTGTGTTTCTAACAAG+TGG - chr3_4:783412-783431 Msa0483930:CDS 40.0%
ATCGCTCCCTTCAAAATTCC+AGG - chr3_4:782840-782859 Msa0483930:CDS 45.0%
! AAGATGGTGGTGCAGTTTGT+GGG + chr3_4:783132-783151 None:intergenic 45.0%
CTCTGTTCAAAGGAAGATGG+TGG + chr3_4:783145-783164 None:intergenic 45.0%
! TGGCTCTGTTCAAAGGAAGA+TGG + chr3_4:783148-783167 None:intergenic 45.0%
TCTACAACACCAAGAAACGC+CGG - chr3_4:783200-783219 Msa0483930:CDS 45.0%
TTGTTGCATATATCTCGCGG+AGG - chr3_4:783283-783302 Msa0483930:CDS 45.0%
TATATCTCGCGGAGGTTATG+AGG - chr3_4:783291-783310 Msa0483930:CDS 45.0%
ATATCTCGCGGAGGTTATGA+GGG - chr3_4:783292-783311 Msa0483930:CDS 45.0%
! GTTTTTCCCTTTTAGCGCCA+CGG + chr3_4:783345-783364 None:intergenic 45.0%
ATCGCTCCCTTCAAAATTCC+AGG - chr3_4:782840-782859 Msa0483930:CDS 45.0%
! AAGATGGTGGTGCAGTTTGT+GGG + chr3_4:783132-783151 None:intergenic 45.0%
CTCTGTTCAAAGGAAGATGG+TGG + chr3_4:783145-783164 None:intergenic 45.0%
! TGGCTCTGTTCAAAGGAAGA+TGG + chr3_4:783148-783167 None:intergenic 45.0%
TCTACAACACCAAGAAACGC+CGG - chr3_4:783200-783219 Msa0483930:CDS 45.0%
TTGTTGCATATATCTCGCGG+AGG - chr3_4:783283-783302 Msa0483930:CDS 45.0%
TATATCTCGCGGAGGTTATG+AGG - chr3_4:783291-783310 Msa0483930:CDS 45.0%
ATATCTCGCGGAGGTTATGA+GGG - chr3_4:783292-783311 Msa0483930:CDS 45.0%
! GTTTTTCCCTTTTAGCGCCA+CGG + chr3_4:783345-783364 None:intergenic 45.0%
!! GAAACGGAAAGAAGGGTACC+TGG + chr3_4:782861-782880 None:intergenic 50.0%
! GAAGATGGTGGTGCAGTTTG+TGG + chr3_4:783133-783152 None:intergenic 50.0%
TGAATCACCGTGGCGCTAAA+AGG - chr3_4:783335-783354 Msa0483930:CDS 50.0%
GAATCACCGTGGCGCTAAAA+GGG - chr3_4:783336-783355 Msa0483930:CDS 50.0%
!! GAAACGGAAAGAAGGGTACC+TGG + chr3_4:782861-782880 None:intergenic 50.0%
! GAAGATGGTGGTGCAGTTTG+TGG + chr3_4:783133-783152 None:intergenic 50.0%
TGAATCACCGTGGCGCTAAA+AGG - chr3_4:783335-783354 Msa0483930:CDS 50.0%
GAATCACCGTGGCGCTAAAA+GGG - chr3_4:783336-783355 Msa0483930:CDS 50.0%
TTCTTCTTCTTCGACGCGGC+CGG + chr3_4:783222-783241 None:intergenic 55.0%
TTCTTCTTCTTCGACGCGGC+CGG + chr3_4:783222-783241 None:intergenic 55.0%
TCGACGCGGCCGGCGTTTCT+TGG + chr3_4:783212-783231 None:intergenic 70.0%
TCGACGCGGCCGGCGTTTCT+TGG + chr3_4:783212-783231 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 782767 783675 782767 ID=Msa0483930;Name=Msa0483930
chr3_4 mRNA 782767 783675 782767 ID=Msa0483930-mRNA-1;Parent=Msa0483930;Name=Msa0483930-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|302
chr3_4 exon 782767 783675 782767 ID=Msa0483930-mRNA-1:exon:234;Parent=Msa0483930-mRNA-1
chr3_4 CDS 782767 783675 782767 ID=Msa0483930-mRNA-1:cds;Parent=Msa0483930-mRNA-1
Gene Sequence

>Msa0483930

ATGTATTCTTCTTCTTCTTCTTCATCTCCATCTCCTTCTCATGATCATGAACCAAAAACCAATGATGTTTATCATCGCTCCCTTCAAAATTCCAGGTACCCTTCTTTCCGTTTCCTTGATTACTCAAACCCTATTCAGAATGAACTCAAAAATCCATCTTCAACAACTAATGATGCTGGAAAACAACAGAACAACGTGAAGAAGATCGTATGGTTTTTTTCTTATTCTGTTCTTGGTAATCATAAGGATGTTGAAAAACAGAACAAACATACATACTCATACTCATACTCATACTCATGTGATGTTTGCAACAAGGTTTTTACTTCTAACAAAGCATTGAATGGACATAAGGGTGTTATTCACCCACAAACTGCACCACCATCTTCCTTTGAACAGAGCCATATTGACTGTTCAAAGTACTTGCCGCCAATATTCTACAACACCAAGAAACGCCGGCCGCGTCGAAGAAGAAGAAGATATATGAACATTGATGATGATGTGATTGCTGCTGAAACTTTGTTGCATATATCTCGCGGAGGTTATGAGGGATCATCTGTCAATATCGATGTGAATCACCGTGGCGCTAAAAGGGAAAAACTTTCTTGCAATATGATGGATGATGATGAGAAGAAGAAGAAGAAGGAACAAGGTGTGTTTCTAACAAGTGGCGATATTGATGAAACATTGAAAGATAATAGCCACAATGAAAAGAAGTTGGTGTTGAGGTTCAAGATTCCTAAAGGCAAGATTTTTCAAACATCACAAGATTGCAAGGAACCTTCTACCTCTAATCATGCTATTGAAGAGAAGAAGATTAATGAAGGTGTTACTGAATTAGGGTCAAAAGTTGTGAAGAATTTTGATTTGAACGAGCTTCCAAGTGATGATTTTGAAGATGAAACTAATTAG

Protein sequence

>Msa0483930

MYSSSSSSSPSPSHDHEPKTNDVYHRSLQNSRYPSFRFLDYSNPIQNELKNPSSTTNDAGKQQNNVKKIVWFFSYSVLGNHKDVEKQNKHTYSYSYSYSCDVCNKVFTSNKALNGHKGVIHPQTAPPSSFEQSHIDCSKYLPPIFYNTKKRRPRRRRRRYMNIDDDVIAAETLLHISRGGYEGSSVNIDVNHRGAKREKLSCNMMDDDEKKKKKEQGVFLTSGDIDETLKDNSHNEKKLVLRFKIPKGKIFQTSQDCKEPSTSNHAIEEKKINEGVTELGSKVVKNFDLNELPSDDFEDETN*