AlfalfaGEDB | Msa0488190

M. sativa cultivar zhongmu-4 / Msa0488190

KEGG: K20791
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004596 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0006464 GO:0006473 GO:0006474 GO:0006475 GO:0006807 GO:0006950 GO:0007275 GO:0008080 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010035 GO:0010154 GO:0010467 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747 GO:0017198 GO:0018002 GO:0018193 GO:0018200 GO:0018209 GO:0019538 GO:0022414 GO:0022626 GO:0030920 GO:0031248 GO:0031365 GO:0031414 GO:0031415 GO:0032501 GO:0032502 GO:0032991 GO:0034212 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0043543 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051604 GO:0061458 GO:0071704 GO:1901564 GO:1901700 GO:1902493 GO:1902494 GO:1990189 GO:1990190 GO:1990234 GO:1990904
PFAM: PF00583.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0488190	GAU38899.1	58.273	139	46	3	4	130	2	140	8.93e-42	147

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0488190	sp\|Q9FKI4\|NAA10_ARATH	51.079	139	56	3	4	130	2	140	3.01e-38	130

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0488190	A0A2Z6P5E3	58.273	139	46	3	4	130	2	140	4.27e-42	147

TFs/TRs:

Gene ID	Type	Classification
Msa0488190	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0488190	MtrunA17_Chr3g0090311	57.554	139	47	3	4	130	2	140	6.33e-45	144

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0488190	AT5G13780.1	51.079	139	56	3	4	130	2	140	3.06e-39	130

Find 31 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTGTTCCATGTCGTTT+TGG	0.275526	3_4:-7472556	None:intergenic
CACGTCGTTGAAGATATTAA+TGG	0.376158	3_4:+7472410	Msa0488190:CDS
TCTAAGATGTACAAACTCTT+TGG	0.380946	3_4:+7472347	Msa0488190:CDS
ACATTGGGATACAAGATTCA+TGG	0.416619	3_4:+7472866	Msa0488190:CDS
CTCCCAAAACGACATGGAAC+AGG	0.433249	3_4:+7472553	Msa0488190:CDS
TTGTATTTAACACAGATGTT+TGG	0.469950	3_4:+7472779	Msa0488190:intron
GGGATACAAGATTCATGGAT+TGG	0.477746	3_4:+7472871	Msa0488190:CDS
TAATTTGTTCAAAGAGACAT+TGG	0.499278	3_4:+7472850	Msa0488190:CDS
TCTCTGATGGGTTGGGAGAA+CGG	0.508987	3_4:-7472501	None:intergenic
AGCTTGTTGCCCTCTTCTCA+CGG	0.516174	3_4:+7472375	Msa0488190:CDS
TACCACGTCTCTGATGGGTT+GGG	0.526888	3_4:-7472508	None:intergenic
AATTTGTTCAAAGAGACATT+GGG	0.527731	3_4:+7472851	Msa0488190:CDS
TAGCGATACCACGTCTCTGA+TGG	0.539259	3_4:-7472514	None:intergenic
ATACCACGTCTCTGATGGGT+TGG	0.540598	3_4:-7472509	None:intergenic
GAGTTGAGGCCGTGAGAAGA+GGG	0.546949	3_4:-7472384	None:intergenic
TGAACAAATTAAAAGCTGCA+TGG	0.554368	3_4:-7472838	None:intergenic
ATGGGTTGGGAGAACGGCGA+GGG	0.570608	3_4:-7472495	None:intergenic
TGAGTTGAGGCCGTGAGAAG+AGG	0.574852	3_4:-7472385	None:intergenic
AGCGATACCACGTCTCTGAT+GGG	0.588160	3_4:-7472513	None:intergenic
GATTGCCTCCCAAAACGACA+TGG	0.596635	3_4:+7472547	Msa0488190:CDS
ATTCAAGAGGAAATTAGTGA+AGG	0.600654	3_4:+7472461	Msa0488190:CDS
GATGGGTTGGGAGAACGGCG+AGG	0.612867	3_4:-7472496	None:intergenic
CGTCCACTGTGAAATTCAAG+AGG	0.617845	3_4:+7472448	Msa0488190:CDS
ATACAAGATTCATGGATTGG+AGG	0.623983	3_4:+7472874	Msa0488190:CDS
TTGAATTTCACAGTGGACGT+AGG	0.625067	3_4:-7472444	None:intergenic
AGAGTTTGTACATCTTAGAG+AGG	0.637261	3_4:-7472343	None:intergenic
CTCCCAACCCATCAGAGACG+TGG	0.645269	3_4:+7472506	Msa0488190:CDS
TCAACGACGTGAATGAGTTG+AGG	0.659923	3_4:-7472398	None:intergenic
ACATCTTAGAGAGGTCGCAA+AGG	0.660670	3_4:-7472334	None:intergenic
TTTCCTCTTGAATTTCACAG+TGG	0.694893	3_4:-7472451	None:intergenic
TTTATGCTGATGATCATGGG+CGG	0.705113	3_4:+7472903	Msa0488190:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTGTATTTAACACAGATGTT+TGG	+	chr3_4:7472779-7472798	Msa0488190:intron	25.0%
!!!	TAATTTGTTCAAAGAGACAT+TGG	+	chr3_4:7472850-7472869	Msa0488190:CDS	25.0%
!!	AATTTGTTCAAAGAGACATT+GGG	+	chr3_4:7472851-7472870	Msa0488190:CDS	25.0%
!	TCTAAGATGTACAAACTCTT+TGG	+	chr3_4:7472347-7472366	Msa0488190:CDS	30.0%
	ATTCAAGAGGAAATTAGTGA+AGG	+	chr3_4:7472461-7472480	Msa0488190:CDS	30.0%
	TATTAATTCAAATTGCACCG+AGG	+	chr3_4:7472628-7472647	Msa0488190:intron	30.0%
	ATTAATTCAAATTGCACCGA+GGG	+	chr3_4:7472629-7472648	Msa0488190:intron	30.0%
	TTTGTGTGTCTTCTTGTAAT+AGG	+	chr3_4:7472724-7472743	Msa0488190:intron	30.0%
	TTGTGTGTCTTCTTGTAATA+GGG	+	chr3_4:7472725-7472744	Msa0488190:intron	30.0%
	TGAACAAATTAAAAGCTGCA+TGG	-	chr3_4:7472841-7472860	None:intergenic	30.0%
!!	AAGTTTTATGCTGATGATCA+TGG	+	chr3_4:7472899-7472918	Msa0488190:CDS	30.0%
!!	AGTTTTATGCTGATGATCAT+GGG	+	chr3_4:7472900-7472919	Msa0488190:CDS	30.0%
	AGAGTTTGTACATCTTAGAG+AGG	-	chr3_4:7472346-7472365	None:intergenic	35.0%
	CACGTCGTTGAAGATATTAA+TGG	+	chr3_4:7472410-7472429	Msa0488190:CDS	35.0%
	TTTCCTCTTGAATTTCACAG+TGG	-	chr3_4:7472454-7472473	None:intergenic	35.0%
	ACATTGGGATACAAGATTCA+TGG	+	chr3_4:7472866-7472885	Msa0488190:CDS	35.0%
!!	ATACAAGATTCATGGATTGG+AGG	+	chr3_4:7472874-7472893	Msa0488190:CDS	35.0%
	TTGAATTTCACAGTGGACGT+AGG	-	chr3_4:7472447-7472466	None:intergenic	40.0%
!!!	AACCTGTTCCATGTCGTTTT+GGG	-	chr3_4:7472558-7472577	None:intergenic	40.0%
!!	AAACCTGTTCCATGTCGTTT+TGG	-	chr3_4:7472559-7472578	None:intergenic	40.0%
	GAAACTGAGTTTGAATCCCT+CGG	-	chr3_4:7472648-7472667	None:intergenic	40.0%
!	CTCAGTTTCTGAGAGTTACA+AGG	+	chr3_4:7472659-7472678	Msa0488190:intron	40.0%
	GGGATACAAGATTCATGGAT+TGG	+	chr3_4:7472871-7472890	Msa0488190:CDS	40.0%
	TTTATGCTGATGATCATGGG+CGG	+	chr3_4:7472903-7472922	Msa0488190:CDS	40.0%
	ACATCTTAGAGAGGTCGCAA+AGG	-	chr3_4:7472337-7472356	None:intergenic	45.0%
	TCAACGACGTGAATGAGTTG+AGG	-	chr3_4:7472401-7472420	None:intergenic	45.0%
	CGTCCACTGTGAAATTCAAG+AGG	+	chr3_4:7472448-7472467	Msa0488190:CDS	45.0%
!!	AGCTTGTTGCCCTCTTCTCA+CGG	+	chr3_4:7472375-7472394	Msa0488190:CDS	50.0%
!!	TCTCTGATGGGTTGGGAGAA+CGG	-	chr3_4:7472504-7472523	None:intergenic	50.0%
!!	TACCACGTCTCTGATGGGTT+GGG	-	chr3_4:7472511-7472530	None:intergenic	50.0%
!	ATACCACGTCTCTGATGGGT+TGG	-	chr3_4:7472512-7472531	None:intergenic	50.0%
	AGCGATACCACGTCTCTGAT+GGG	-	chr3_4:7472516-7472535	None:intergenic	50.0%
	TAGCGATACCACGTCTCTGA+TGG	-	chr3_4:7472517-7472536	None:intergenic	50.0%
	GATTGCCTCCCAAAACGACA+TGG	+	chr3_4:7472547-7472566	Msa0488190:CDS	50.0%
!!!	CTGTTCCATGTCGTTTTGGG+AGG	-	chr3_4:7472555-7472574	None:intergenic	50.0%
	CTCCCAAAACGACATGGAAC+AGG	+	chr3_4:7472553-7472572	Msa0488190:CDS	50.0%
	GAGTTGAGGCCGTGAGAAGA+GGG	-	chr3_4:7472387-7472406	None:intergenic	55.0%
!	TGAGTTGAGGCCGTGAGAAG+AGG	-	chr3_4:7472388-7472407	None:intergenic	55.0%
!!	ATGGGTTGGGAGAACGGCGA+GGG	-	chr3_4:7472498-7472517	None:intergenic	60.0%
	CTCCCAACCCATCAGAGACG+TGG	+	chr3_4:7472506-7472525	Msa0488190:CDS	60.0%
!!	GATGGGTTGGGAGAACGGCG+AGG	-	chr3_4:7472499-7472518	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	7472305	7472931	7472305	ID=Msa0488190;Name=Msa0488190
chr3_4	mRNA	7472305	7472931	7472305	ID=Msa0488190-mRNA-1;Parent=Msa0488190;Name=Msa0488190-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|2\|0\|135
chr3_4	exon	7472305	7472574	7472305	ID=Msa0488190-mRNA-1:exon:2584;Parent=Msa0488190-mRNA-1
chr3_4	exon	7472794	7472931	7472794	ID=Msa0488190-mRNA-1:exon:2585;Parent=Msa0488190-mRNA-1
chr3_4	CDS	7472305	7472574	7472305	ID=Msa0488190-mRNA-1:cds;Parent=Msa0488190-mRNA-1
chr3_4	CDS	7472794	7472931	7472794	ID=Msa0488190-mRNA-1:cds;Parent=Msa0488190-mRNA-1

Gene Sequence

>Msa0488190

ATGTCTCAAGTGTGCATAAGAGAAGCAAACCTTTGCGACCTCTCTAAGATGTACAAACTCTTTGGTCTAAAGCTTGTTGCCCTCTTCTCACGGCCTCAACTCATTCACGTCGTTGAAGATATTAATGGTCACATCGTCGCCTACGTCCACTGTGAAATTCAAGAGGAAATTAGTGAAGGAGCTATTCATTCCCTCGCCGTTCTCCCAACCCATCAGAGACGTGGTATCGCTACTAAGCTTGTGATTGCCTCCCAAAACGACATGGAACAGATGTTTGGTTCTGAGTACGTCTCAGTGCATGTCAGAAAGAGCAACCATGCAGCTTTTAATTTGTTCAAAGAGACATTGGGATACAAGATTCATGGATTGGAGGCAAAGTTTTATGCTGATGATCATGGGCGGATCTAG

Protein sequence

>Msa0488190

MSQVCIREANLCDLSKMYKLFGLKLVALFSRPQLIHVVEDINGHIVAYVHCEIQEEISEGAIHSLAVLPTHQRRGIATKLVIASQNDMEQMFGSEYVSVHVRKSNHAAFNLFKETLGYKIHGLEAKFYADDHGRI*