Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0489020 | XP_013459208.1 | 90.545 | 275 | 21 | 2 | 1 | 270 | 1 | 275 | 7.06e-173 | 488 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0489020 | sp|Q39092|ZAT1_ARATH | 26.296 | 270 | 148 | 9 | 5 | 263 | 4 | 233 | 1.56e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0489020 | A0A072UTD1 | 90.545 | 275 | 21 | 2 | 1 | 270 | 1 | 275 | 3.37e-173 | 488 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0489020 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0489020 | MtrunA17_Chr3g0079131 | 90.545 | 275 | 21 | 2 | 1 | 270 | 1 | 275 | 6.49e-177 | 488 |
| Msa0489020 | MtrunA17_Chr3g0079061 | 62.182 | 275 | 99 | 2 | 1 | 270 | 1 | 275 | 1.52e-113 | 327 |
| Msa0489020 | MtrunA17_Chr3g0079091 | 62.712 | 177 | 61 | 2 | 1 | 172 | 1 | 177 | 4.61e-63 | 196 |
| Msa0489020 | MtrunA17_Chr4g0018761 | 29.104 | 268 | 151 | 7 | 3 | 264 | 4 | 238 | 1.99e-25 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0489020 | AT1G02030.1 | 26.296 | 270 | 148 | 9 | 5 | 263 | 4 | 233 | 1.59e-12 | 66.2 |
| Msa0489020 | AT3G60580.1 | 27.240 | 279 | 142 | 11 | 7 | 261 | 5 | 246 | 2.82e-11 | 63.2 |
Find 25 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAAGATTATGCATAGGTTT+TGG | 0.234409 | 3_4:-9168828 | None:intergenic |
| CGGTCGTAGACAATTTAATT+TGG | 0.272723 | 3_4:+9168950 | Msa0489020:CDS |
| AACAATATATCTGAAGAAAT+TGG | 0.302487 | 3_4:+9169275 | Msa0489020:CDS |
| TGAGAATACACAAGTTAAAT+TGG | 0.302912 | 3_4:+9169049 | Msa0489020:CDS |
| ATACTATAGTGAGAAGAAAA+AGG | 0.312924 | 3_4:+9169151 | Msa0489020:CDS |
| TAAATTGGTGTATAGTGATT+TGG | 0.323391 | 3_4:+9169064 | Msa0489020:CDS |
| TCAGGTGCTTGGTTGTTGAT+TGG | 0.373001 | 3_4:-9168738 | None:intergenic |
| TCTGAAGAAATTGGTATAAG+TGG | 0.418644 | 3_4:+9169284 | Msa0489020:CDS |
| CAACTGAGTGTTCAGGTGCT+TGG | 0.424891 | 3_4:-9168749 | None:intergenic |
| CTATTATTTGTGTGAATGAA+TGG | 0.435873 | 3_4:+9169111 | Msa0489020:CDS |
| GTTGACTCAACTGAGTGTTC+AGG | 0.442669 | 3_4:-9168756 | None:intergenic |
| AATAGAGAGAGTTTGATGTT+TGG | 0.448411 | 3_4:-9168778 | None:intergenic |
| TATTCTTACTAAAGTTTACA+AGG | 0.462564 | 3_4:-9168878 | None:intergenic |
| TCACAAGTGCACATATTGCT+TGG | 0.480968 | 3_4:+9169337 | Msa0489020:CDS |
| CAATGCTTTCCCATTAGAAA+AGG | 0.484168 | 3_4:-9168664 | None:intergenic |
| TACAGAAGCTGCTGAAACAA+TGG | 0.521106 | 3_4:+9169088 | Msa0489020:CDS |
| TGTACTTGAGGAACACAAAA+CGG | 0.523616 | 3_4:+9169379 | Msa0489020:CDS |
| TGAATCTGATCATGTACTTG+AGG | 0.537940 | 3_4:+9169367 | Msa0489020:CDS |
| ATAATCTTCGATCTTTGAAG+AGG | 0.557615 | 3_4:+9168841 | Msa0489020:CDS |
| AGAAATTGGTATAAGTGGAA+AGG | 0.574832 | 3_4:+9169289 | Msa0489020:CDS |
| AAAGATCGAAGATTATGCAT+AGG | 0.576327 | 3_4:-9168834 | None:intergenic |
| TGTAGCTGAAGAAAATGAAG+AGG | 0.590626 | 3_4:+9168995 | Msa0489020:CDS |
| ATTCTTACTAAAGTTTACAA+GGG | 0.609953 | 3_4:-9168877 | None:intergenic |
| TCTAATGGGAAAGCATTGGG+AGG | 0.669450 | 3_4:+9168669 | Msa0489020:CDS |
| GAATGAATGGAAACAAGTTG+AGG | 0.698163 | 3_4:+9169124 | Msa0489020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATATTGTTATTTTTCTTA+TGG | - | chr3_4:9169265-9169284 | None:intergenic | 10.0% |
| !!! | ATATATTGTTATTTTTCTTA+TGG | - | chr3_4:9169265-9169284 | None:intergenic | 10.0% |
| !! | ATTCTTACTAAAGTTTACAA+GGG | - | chr3_4:9168880-9168899 | None:intergenic | 20.0% |
| !! | TATTCTTACTAAAGTTTACA+AGG | - | chr3_4:9168881-9168900 | None:intergenic | 20.0% |
| !! | AACAATATATCTGAAGAAAT+TGG | + | chr3_4:9169275-9169294 | Msa0489020:CDS | 20.0% |
| !! | ATTCTTACTAAAGTTTACAA+GGG | - | chr3_4:9168880-9168899 | None:intergenic | 20.0% |
| !! | TATTCTTACTAAAGTTTACA+AGG | - | chr3_4:9168881-9168900 | None:intergenic | 20.0% |
| !! | AACAATATATCTGAAGAAAT+TGG | + | chr3_4:9169275-9169294 | Msa0489020:CDS | 20.0% |
| ! | TGAGAATACACAAGTTAAAT+TGG | + | chr3_4:9169049-9169068 | Msa0489020:CDS | 25.0% |
| ! | TAAATTGGTGTATAGTGATT+TGG | + | chr3_4:9169064-9169083 | Msa0489020:CDS | 25.0% |
| ! | CTATTATTTGTGTGAATGAA+TGG | + | chr3_4:9169111-9169130 | Msa0489020:CDS | 25.0% |
| ! | ATACTATAGTGAGAAGAAAA+AGG | + | chr3_4:9169151-9169170 | Msa0489020:CDS | 25.0% |
| ! | AAGAAAAAGGTTAGTGAAAA+TGG | + | chr3_4:9169164-9169183 | Msa0489020:CDS | 25.0% |
| ! | TGAGAATACACAAGTTAAAT+TGG | + | chr3_4:9169049-9169068 | Msa0489020:CDS | 25.0% |
| ! | TAAATTGGTGTATAGTGATT+TGG | + | chr3_4:9169064-9169083 | Msa0489020:CDS | 25.0% |
| ! | CTATTATTTGTGTGAATGAA+TGG | + | chr3_4:9169111-9169130 | Msa0489020:CDS | 25.0% |
| ! | ATACTATAGTGAGAAGAAAA+AGG | + | chr3_4:9169151-9169170 | Msa0489020:CDS | 25.0% |
| ! | AAGAAAAAGGTTAGTGAAAA+TGG | + | chr3_4:9169164-9169183 | Msa0489020:CDS | 25.0% |
| ! | TGTAACAAGTCCTTTTCTAA+TGG | + | chr3_4:9168654-9168673 | Msa0489020:CDS | 30.0% |
| ! | GTAACAAGTCCTTTTCTAAT+GGG | + | chr3_4:9168655-9168674 | Msa0489020:CDS | 30.0% |
| !!! | TTTTCTAATGGGAAAGCATT+GGG | + | chr3_4:9168666-9168685 | Msa0489020:CDS | 30.0% |
| !! | TGTTGATTGGAGATTTTAGA+GGG | - | chr3_4:9168728-9168747 | None:intergenic | 30.0% |
| !! | TTGTTGATTGGAGATTTTAG+AGG | - | chr3_4:9168729-9168748 | None:intergenic | 30.0% |
| AATAGAGAGAGTTTGATGTT+TGG | - | chr3_4:9168781-9168800 | None:intergenic | 30.0% | |
| !! | TTTTGGATTGGAAATTGATG+AGG | - | chr3_4:9168814-9168833 | None:intergenic | 30.0% |
| !!! | ATTATGCATAGGTTTTGGAT+TGG | - | chr3_4:9168826-9168845 | None:intergenic | 30.0% |
| AAAGATCGAAGATTATGCAT+AGG | - | chr3_4:9168837-9168856 | None:intergenic | 30.0% | |
| ! | ATAATCTTCGATCTTTGAAG+AGG | + | chr3_4:9168841-9168860 | Msa0489020:CDS | 30.0% |
| TCTGAAGAAATTGGTATAAG+TGG | + | chr3_4:9169284-9169303 | Msa0489020:CDS | 30.0% | |
| AGAAATTGGTATAAGTGGAA+AGG | + | chr3_4:9169289-9169308 | Msa0489020:CDS | 30.0% | |
| ! | TGGGTTAGAATCAGAAAAAT+TGG | - | chr3_4:9169414-9169433 | None:intergenic | 30.0% |
| ! | TGTAACAAGTCCTTTTCTAA+TGG | + | chr3_4:9168654-9168673 | Msa0489020:CDS | 30.0% |
| ! | GTAACAAGTCCTTTTCTAAT+GGG | + | chr3_4:9168655-9168674 | Msa0489020:CDS | 30.0% |
| !!! | TTTTCTAATGGGAAAGCATT+GGG | + | chr3_4:9168666-9168685 | Msa0489020:CDS | 30.0% |
| !! | TGTTGATTGGAGATTTTAGA+GGG | - | chr3_4:9168728-9168747 | None:intergenic | 30.0% |
| !! | TTGTTGATTGGAGATTTTAG+AGG | - | chr3_4:9168729-9168748 | None:intergenic | 30.0% |
| AATAGAGAGAGTTTGATGTT+TGG | - | chr3_4:9168781-9168800 | None:intergenic | 30.0% | |
| !! | TTTTGGATTGGAAATTGATG+AGG | - | chr3_4:9168814-9168833 | None:intergenic | 30.0% |
| !!! | ATTATGCATAGGTTTTGGAT+TGG | - | chr3_4:9168826-9168845 | None:intergenic | 30.0% |
| AAAGATCGAAGATTATGCAT+AGG | - | chr3_4:9168837-9168856 | None:intergenic | 30.0% | |
| ! | ATAATCTTCGATCTTTGAAG+AGG | + | chr3_4:9168841-9168860 | Msa0489020:CDS | 30.0% |
| TCTGAAGAAATTGGTATAAG+TGG | + | chr3_4:9169284-9169303 | Msa0489020:CDS | 30.0% | |
| AGAAATTGGTATAAGTGGAA+AGG | + | chr3_4:9169289-9169308 | Msa0489020:CDS | 30.0% | |
| ! | TGGGTTAGAATCAGAAAAAT+TGG | - | chr3_4:9169414-9169433 | None:intergenic | 30.0% |
| CAATGCTTTCCCATTAGAAA+AGG | - | chr3_4:9168667-9168686 | None:intergenic | 35.0% | |
| !! | CTTTTCTAATGGGAAAGCAT+TGG | + | chr3_4:9168665-9168684 | Msa0489020:CDS | 35.0% |
| ! | TAGCTAAGTGAGTTTTCATG+TGG | - | chr3_4:9168695-9168714 | None:intergenic | 35.0% |
| !! | ATTGGAGATTTTAGAGGGAT+TGG | - | chr3_4:9168723-9168742 | None:intergenic | 35.0% |
| ! | CGAAGATTATGCATAGGTTT+TGG | - | chr3_4:9168831-9168850 | None:intergenic | 35.0% |
| ! | TCTTTTCCAAAAAACCCAAC+TGG | + | chr3_4:9168912-9168931 | Msa0489020:CDS | 35.0% |
| ! | CTTTTTCCAGTTGGGTTTTT+TGG | - | chr3_4:9168921-9168940 | None:intergenic | 35.0% |
| ! | GTTTCGATCTTTTTCCAGTT+GGG | - | chr3_4:9168929-9168948 | None:intergenic | 35.0% |
| CGGTCGTAGACAATTTAATT+TGG | + | chr3_4:9168950-9168969 | Msa0489020:CDS | 35.0% | |
| TGTAGCTGAAGAAAATGAAG+AGG | + | chr3_4:9168995-9169014 | Msa0489020:CDS | 35.0% | |
| GAATGAATGGAAACAAGTTG+AGG | + | chr3_4:9169124-9169143 | Msa0489020:CDS | 35.0% | |
| TGAATCTGATCATGTACTTG+AGG | + | chr3_4:9169367-9169386 | Msa0489020:CDS | 35.0% | |
| TGTACTTGAGGAACACAAAA+CGG | + | chr3_4:9169379-9169398 | Msa0489020:CDS | 35.0% | |
| CAATGCTTTCCCATTAGAAA+AGG | - | chr3_4:9168667-9168686 | None:intergenic | 35.0% | |
| !! | CTTTTCTAATGGGAAAGCAT+TGG | + | chr3_4:9168665-9168684 | Msa0489020:CDS | 35.0% |
| ! | TAGCTAAGTGAGTTTTCATG+TGG | - | chr3_4:9168695-9168714 | None:intergenic | 35.0% |
| !! | ATTGGAGATTTTAGAGGGAT+TGG | - | chr3_4:9168723-9168742 | None:intergenic | 35.0% |
| ! | CGAAGATTATGCATAGGTTT+TGG | - | chr3_4:9168831-9168850 | None:intergenic | 35.0% |
| ! | TCTTTTCCAAAAAACCCAAC+TGG | + | chr3_4:9168912-9168931 | Msa0489020:CDS | 35.0% |
| ! | CTTTTTCCAGTTGGGTTTTT+TGG | - | chr3_4:9168921-9168940 | None:intergenic | 35.0% |
| ! | GTTTCGATCTTTTTCCAGTT+GGG | - | chr3_4:9168929-9168948 | None:intergenic | 35.0% |
| CGGTCGTAGACAATTTAATT+TGG | + | chr3_4:9168950-9168969 | Msa0489020:CDS | 35.0% | |
| TGTAGCTGAAGAAAATGAAG+AGG | + | chr3_4:9168995-9169014 | Msa0489020:CDS | 35.0% | |
| GAATGAATGGAAACAAGTTG+AGG | + | chr3_4:9169124-9169143 | Msa0489020:CDS | 35.0% | |
| TGAATCTGATCATGTACTTG+AGG | + | chr3_4:9169367-9169386 | Msa0489020:CDS | 35.0% | |
| TGTACTTGAGGAACACAAAA+CGG | + | chr3_4:9169379-9169398 | Msa0489020:CDS | 35.0% | |
| ! | CGTTTCGATCTTTTTCCAGT+TGG | - | chr3_4:9168930-9168949 | None:intergenic | 40.0% |
| ACTGGAAAAAGATCGAAACG+CGG | + | chr3_4:9168930-9168949 | Msa0489020:CDS | 40.0% | |
| TACAGAAGCTGCTGAAACAA+TGG | + | chr3_4:9169088-9169107 | Msa0489020:CDS | 40.0% | |
| TCACAAGTGCACATATTGCT+TGG | + | chr3_4:9169337-9169356 | Msa0489020:CDS | 40.0% | |
| ! | CGTTTCGATCTTTTTCCAGT+TGG | - | chr3_4:9168930-9168949 | None:intergenic | 40.0% |
| ACTGGAAAAAGATCGAAACG+CGG | + | chr3_4:9168930-9168949 | Msa0489020:CDS | 40.0% | |
| TACAGAAGCTGCTGAAACAA+TGG | + | chr3_4:9169088-9169107 | Msa0489020:CDS | 40.0% | |
| TCACAAGTGCACATATTGCT+TGG | + | chr3_4:9169337-9169356 | Msa0489020:CDS | 40.0% | |
| !! | TCTAATGGGAAAGCATTGGG+AGG | + | chr3_4:9168669-9168688 | Msa0489020:CDS | 45.0% |
| TCAGGTGCTTGGTTGTTGAT+TGG | - | chr3_4:9168741-9168760 | None:intergenic | 45.0% | |
| ! | GTTGACTCAACTGAGTGTTC+AGG | - | chr3_4:9168759-9168778 | None:intergenic | 45.0% |
| !! | TCTAATGGGAAAGCATTGGG+AGG | + | chr3_4:9168669-9168688 | Msa0489020:CDS | 45.0% |
| TCAGGTGCTTGGTTGTTGAT+TGG | - | chr3_4:9168741-9168760 | None:intergenic | 45.0% | |
| ! | GTTGACTCAACTGAGTGTTC+AGG | - | chr3_4:9168759-9168778 | None:intergenic | 45.0% |
| CAACTGAGTGTTCAGGTGCT+TGG | - | chr3_4:9168752-9168771 | None:intergenic | 50.0% | |
| CAACTGAGTGTTCAGGTGCT+TGG | - | chr3_4:9168752-9168771 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 9168624 | 9169436 | 9168624 | ID=Msa0489020;Name=Msa0489020 |
| chr3_4 | mRNA | 9168624 | 9169436 | 9168624 | ID=Msa0489020-mRNA-1;Parent=Msa0489020;Name=Msa0489020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|270 |
| chr3_4 | exon | 9168624 | 9169436 | 9168624 | ID=Msa0489020-mRNA-1:exon:2999;Parent=Msa0489020-mRNA-1 |
| chr3_4 | CDS | 9168624 | 9169436 | 9168624 | ID=Msa0489020-mRNA-1:cds;Parent=Msa0489020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
