AlfalfaGEDB | Msa0491670

M. sativa cultivar zhongmu-4 / Msa0491670

KEGG: -
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0491670	KEH17177.1	82.353	102	17	1	1	101	1	102	1.05e-52	175

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0491670	sp\|Q9LVS0\|KUA1_ARATH	78.261	69	15	0	26	94	95	163	7.33e-35	126

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0491670	A0A072TIE3	82.353	102	17	1	1	101	1	102	4.99e-53	175

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TFs/TRs:

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Gene ID	Type	Classification
Msa0491670	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0491670	MtrunA17_Chr3g0092621	83.333	72	12	0	23	94	80	151	5.46e-38	126
Msa0491670	MtrunA17_Chr4g0055931	76.812	69	16	0	26	94	94	162	3.45e-35	123
Msa0491670	MtrunA17_Chr6g0488471	78.788	66	14	0	26	91	96	161	7.59e-35	121
Msa0491670	MtrunA17_Chr2g0331031	73.913	69	18	0	26	94	108	176	1.37e-34	122
Msa0491670	MtrunA17_Chr8g0389241	55.140	107	35	3	6	105	89	189	7.63e-33	116
Msa0491670	MtrunA17_Chr5g0415191	87.143	70	9	0	26	95	101	170	1.17e-32	116
Msa0491670	MtrunA17_Chr4g0059701	95.714	70	3	0	26	95	83	152	4.87e-32	114
Msa0491670	MtrunA17_Chr4g0062741	72.059	68	19	0	26	93	137	204	1.20e-31	113
Msa0491670	MtrunA17_Chr1g0162081	70.588	68	20	0	26	93	121	188	2.01e-30	110
Msa0491670	MtrunA17_Chr1g0210471	69.118	68	21	0	26	93	123	190	1.00e-29	107

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0491670	AT5G47390.1	78.261	69	15	0	26	94	95	163	7.45e-36	126
Msa0491670	AT1G49010.1	73.529	68	18	0	26	93	133	200	5.41e-32	115
Msa0491670	AT1G70000.1	83.099	71	12	0	26	96	97	167	7.30e-31	110
Msa0491670	AT1G70000.2	83.099	71	12	0	26	96	97	167	7.30e-31	110
Msa0491670	AT5G04760.1	58.333	84	35	0	26	109	98	181	1.29e-30	108
Msa0491670	AT2G38090.1	69.118	68	21	0	26	93	140	207	7.19e-30	109
Msa0491670	AT5G08520.1	69.118	68	21	0	26	93	117	184	8.26e-30	108
Msa0491670	AT5G56840.1	68.116	69	21	1	26	93	90	158	1.00e-29	107
Msa0491670	AT5G58900.1	69.118	68	21	0	26	93	140	207	1.78e-29	107
Msa0491670	AT3G16350.1	75.362	69	17	0	26	94	136	204	6.80e-29	108

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Find 19 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAAGTGGGAAAAGGTGAT+TGG	0.482958	3_4:-14533297	Msa0491670:CDS
ATCAGGGATTCCGTGGACTG+AGG	0.660333	3_4:-14533350	Msa0491670:intron
CTTTGATATCACCACCGACA+CGG	0.638033	3_4:-14533158	Msa0491670:intron
GACGACGATTGAGATTACTT+CGG	0.681636	3_4:+14533197	None:intergenic
GACGATTGAGATTACTTCGG+CGG	0.616099	3_4:+14533200	None:intergenic
GCATGACTTGCAACATGCGT+AGG	0.636946	3_4:+14533238	None:intergenic
GGATTGGAGAAAGTGGGAAA+AGG	0.562648	3_4:-14533304	Msa0491670:CDS
GGTGGTGATATCAAAGAGAC+TGG	0.540239	3_4:+14533165	None:intergenic
GTGATTGGAGAGGAATCTCA+AGG	0.619264	3_4:-14533282	Msa0491670:CDS
GTGGGAAAAGGTGATTGGAG+AGG	0.624127	3_4:-14533292	Msa0491670:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAATTGACAAAAAACACAC+AGG	+	chr3_4:14532074-14532093	None:intergenic	30.0%
GTGCTGTATTATTCATTTAG+GGG	-	chr3_4:14532150-14532169	Msa0491670:intron	30.0%
ACATGTGCACAAACTTTAAT+TGG	-	chr3_4:14532203-14532222	Msa0491670:intron	30.0%
ATAACTACATCTTATGCAAG+AGG	-	chr3_4:14532227-14532246	Msa0491670:intron	30.0%
GTTCATTATGTTAGTCCTTT+AGG	-	chr3_4:14532968-14532987	Msa0491670:intron	30.0%
CTTACAAATTCAATGTAGCT+AGG	+	chr3_4:14533026-14533045	None:intergenic	30.0%
AATTCAACTTACGGTAAAGT+GGG	+	chr3_4:14533103-14533122	None:intergenic	30.0%
AAATTCAACTTACGGTAAAG+TGG	+	chr3_4:14533104-14533123	None:intergenic	30.0%
TACGAACCAACAATAGTAAA+AGG	+	chr3_4:14533163-14533182	None:intergenic	30.0%
AAGTAAAGCCATGAATCATT+TGG	-	chr3_4:14533253-14533272	Msa0491670:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	14532024	14533844	14532024	ID=Msa0491670;Name=Msa0491670
chr3_4	mRNA	14532024	14533844	14532024	ID=Msa0491670-mRNA-1;Parent=Msa0491670;Name=Msa0491670-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0\|0\|0.33\|0\|0.33\|6\|0\|124
chr3_4	exon	14533769	14533844	14533769	ID=Msa0491670-mRNA-1:exon:4269;Parent=Msa0491670-mRNA-1
chr3_4	exon	14533159	14533367	14533159	ID=Msa0491670-mRNA-1:exon:4268;Parent=Msa0491670-mRNA-1
chr3_4	exon	14533064	14533070	14533064	ID=Msa0491670-mRNA-1:exon:4267;Parent=Msa0491670-mRNA-1
chr3_4	exon	14532243	14532295	14532243	ID=Msa0491670-mRNA-1:exon:4266;Parent=Msa0491670-mRNA-1
chr3_4	exon	14532193	14532199	14532193	ID=Msa0491670-mRNA-1:exon:4265;Parent=Msa0491670-mRNA-1
chr3_4	exon	14532024	14532046	14532024	ID=Msa0491670-mRNA-1:exon:4264;Parent=Msa0491670-mRNA-1
chr3_4	CDS	14533769	14533844	14533769	ID=Msa0491670-mRNA-1:cds;Parent=Msa0491670-mRNA-1
chr3_4	CDS	14533159	14533367	14533159	ID=Msa0491670-mRNA-1:cds;Parent=Msa0491670-mRNA-1
chr3_4	CDS	14533064	14533070	14533064	ID=Msa0491670-mRNA-1:cds;Parent=Msa0491670-mRNA-1
chr3_4	CDS	14532243	14532295	14532243	ID=Msa0491670-mRNA-1:cds;Parent=Msa0491670-mRNA-1
chr3_4	CDS	14532193	14532199	14532193	ID=Msa0491670-mRNA-1:cds;Parent=Msa0491670-mRNA-1
chr3_4	CDS	14532024	14532046	14532024	ID=Msa0491670-mRNA-1:cds;Parent=Msa0491670-mRNA-1

Gene Sequence	>Msa0491670 ATGTTTGTTGCAGAGTTTTTGAGAGCTGTTTTTTGTGTGCCCCCTATCCTGTGTGTTTTTTGTCAATTTGCTTCTGGGATTCCGTGGACTGAGGAAGAGCACAAGATGTTTTTGGTTGGATTGGAGAAAGTGGGAAAAGGTGATTGGAGAGGAATCTCAAGGAACTATGTGAAAACAAGAACGCCTACGCATGTTGCAAGTCATGCTCAGAAGTACTTTCTCCGCCGAAGTAATCTCAATCGTCGTCGCCGTAGATCCAGTCTCTTTGATATCACCACCGACACGTATTATGTTTATTTGACTTCAAATCTAGCTGCATGGTTGGGACTTATGATCTCAAAGCATAACATTGGATTGCATGAAAAAAATGTGTAA
Protein sequence	>Msa0491670 MFVAEFLRAVFCVPPILCVFCQFASGIPWTEEEHKMFLVGLEKVGKGDWRGISRNYVKTRTPTHVASHAQKYFLRRSNLNRRRRRSSLFDITTDTYYVYLTSNLAAWLGLMISKHNIGLHEKNV*