AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0494600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0494600 MtrunA17_Chr3g0090311 38.136 118 66 3 5 119 21 134 1.87e-15 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0494600 AT5G13780.1 43.678 87 45 2 33 119 52 134 2.80e-14 67.0

Find 46 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCGAGCTTATTGTTTGTT+TGG 0.285966 3_4:-22831542 Msa0494600:CDS
ACATCGATGATCGGCAGATT+TGG 0.298947 3_4:-22831968 None:intergenic
GATCCAACAGCTCCTGTATC+AGG 0.319631 3_4:-22828813 Msa0494600:CDS
TGTTTGTTTGGAAAGACCTT+AGG 0.354964 3_4:-22831530 Msa0494600:CDS
CGCTATTGAGGACTTGGAAC+GGG 0.400054 3_4:-22831735 Msa0494600:intron
TAAACAATCCTTCTGAGATC+AGG 0.411813 3_4:-22831937 Msa0494600:CDS
TGTCAACTCTATAGTTCATA+AGG 0.425180 3_4:-22831852 Msa0494600:CDS
TACAAATTCAGTGGCTATGA+TGG 0.429790 3_4:+22828839 Msa0494590:CDS
CCGGCACCAGCACCTGATAC+AGG 0.445209 3_4:+22828801 Msa0494590:CDS
GTTTGTTTGGAAAGACCTTA+GGG 0.452496 3_4:-22831529 Msa0494600:CDS
GATGATCGGCAGATTTGGTC+TGG 0.455393 3_4:-22831963 Msa0494600:CDS
CCTGTATCAGGTGCTGGTGC+CGG 0.464757 3_4:-22828801 Msa0494600:CDS
TAAGCTCGAAATTTCTTCCT+CGG 0.470115 3_4:+22831554 None:intergenic
AAGCTCGAAATTTCTTCCTC+GGG 0.485496 3_4:+22831555 None:intergenic
GACCGCCGCTATTGAGGACT+TGG 0.487277 3_4:-22831741 Msa0494600:CDS
GCAGCTCATGCAGTGGTTGG+TGG 0.489191 3_4:+22831898 None:intergenic
ACCTTAGGGTACGATATTCA+TGG 0.489911 3_4:-22831515 Msa0494600:CDS
AGGTGCTGGTGCCGGAGCCA+TGG 0.497768 3_4:-22828793 Msa0494600:CDS
ACTTGTGACCGCCGCTATTG+AGG 0.499579 3_4:-22831747 Msa0494600:CDS
ACAGCTCCTGTATCAGGTGC+TGG 0.501121 3_4:-22828807 Msa0494600:CDS
TGCTGGTGCCGGAGCCATGG+AGG 0.514185 3_4:-22828790 Msa0494600:CDS
CCTCAGTCCCTCCATGGCTC+CGG 0.526166 3_4:+22828782 Msa0494590:CDS
GCCGCAGCTCATGCAGTGGT+TGG 0.537031 3_4:+22831895 None:intergenic
GCACCTGATACAGGAGCTGT+TGG 0.540117 3_4:+22828810 Msa0494590:CDS
TGCGGCCGTGACAAAATTGT+CGG 0.540537 3_4:-22831878 Msa0494600:CDS
GCTGGTGCCGGAGCCATGGA+GGG 0.555223 3_4:-22828789 Msa0494600:CDS
TCAAGACCTCAGTCCCTCCA+TGG 0.563951 3_4:+22828776 Msa0494590:CDS
ACAAATTCAGTGGCTATGAT+GGG 0.565910 3_4:+22828840 Msa0494590:CDS
TACGGAGAACCACGACGTGA+TGG 0.566601 3_4:+22831798 None:intergenic
CACGGCCGCAGCTCATGCAG+TGG 0.567631 3_4:+22831891 None:intergenic
ACCAACCACTGCATGAGCTG+CGG 0.580426 3_4:-22831896 Msa0494600:CDS
TGAAGAAGCCTGATCTCAGA+AGG 0.586919 3_4:+22831929 None:intergenic
AGCTATCATAGACGTGGTAT+CGG 0.590254 3_4:-22831776 Msa0494600:CDS
ACATCTCAGCGCTCGTCCCG+AGG 0.601365 3_4:-22831571 Msa0494600:CDS
ACCATGAATATCGTACCCTA+AGG 0.607373 3_4:+22831514 None:intergenic
CCGGAGCCATGGAGGGACTG+AGG 0.607985 3_4:-22828782 Msa0494600:CDS
TGGATCTCTTACAAATTCAG+TGG 0.623573 3_4:+22828830 Msa0494590:CDS
CATATAACGACATCGATGAT+CGG 0.625866 3_4:-22831977 None:intergenic
CTCCGTAGCTATCATAGACG+TGG 0.628264 3_4:-22831782 Msa0494600:CDS
CCGCTATTGAGGACTTGGAA+CGG 0.633873 3_4:-22831736 Msa0494600:CDS
ACGAGCGCTGAGATGTACTC+AGG 0.636447 3_4:+22831578 None:intergenic
CGCTAAAATCCATCACGTCG+TGG 0.646158 3_4:-22831807 Msa0494600:CDS
TTCCAAGTCCTCAATAGCGG+CGG 0.666332 3_4:+22831739 None:intergenic
TACCACGTCTATGATAGCTA+CGG 0.686642 3_4:+22831780 None:intergenic
CCGTTCCAAGTCCTCAATAG+CGG 0.687217 3_4:+22831736 None:intergenic
GGTGCTGATGTTGAGAACAA+TGG 0.703960 3_4:-22831494 Msa0494600:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGATTTATTCATAAATTT+TGG - chr3_4:22829088-22829107 Msa0494600:intron 10.0%
!!! GTTTTTTTTTTTTAAAACAA+AGG + chr3_4:22830090-22830109 None:intergenic 10.0%
!! AAATTAAAAAATTGTATTGA+AGG + chr3_4:22831256-22831275 None:intergenic 10.0%
!! GTAAATAAATAGAAAGTTAT+CGG + chr3_4:22829832-22829851 None:intergenic 15.0%
!!! AAAATTTGTTAGCTATATAA+TGG + chr3_4:22830038-22830057 None:intergenic 15.0%
!!! TATTGTAATATCAACTTTTT+TGG - chr3_4:22831025-22831044 Msa0494600:intron 15.0%
!!! ATTGTAATATCAACTTTTTT+GGG - chr3_4:22831026-22831045 Msa0494600:intron 15.0%
!!! TAGTTTTGGAAAATTTTATA+AGG + chr3_4:22831233-22831252 None:intergenic 15.0%
!!! AATTTGCTTATAATTGATAA+CGG + chr3_4:22831672-22831691 None:intergenic 15.0%
!!! ATAAGCAAATTTTACTTTTT+AGG - chr3_4:22831681-22831700 Msa0494600:intron 15.0%
!! GAATAATTGAAACAAACAAT+TGG + chr3_4:22829036-22829055 None:intergenic 20.0%
!!! CAATTTATTTTCACAAAAAC+GGG + chr3_4:22829404-22829423 None:intergenic 20.0%
!!! CCAATTTATTTTCACAAAAA+CGG + chr3_4:22829405-22829424 None:intergenic 20.0%
!! AAAATAAATTGGATCTTTCT+TGG - chr3_4:22829413-22829432 Msa0494600:intron 20.0%
!! CATGTTGTAATAAAAAGAAA+CGG + chr3_4:22829733-22829752 None:intergenic 20.0%
!!! CTTTTAGAAAGTGAATTTTT+TGG - chr3_4:22829766-22829785 Msa0494600:intron 20.0%
!! ATATCACATTGAAATTGAAT+CGG + chr3_4:22829870-22829889 None:intergenic 20.0%
!!! ATAGAAATTTGTTGAAAAGA+AGG + chr3_4:22829926-22829945 None:intergenic 20.0%
!!! AAACAAAACAATTTTTCTGA+TGG + chr3_4:22829955-22829974 None:intergenic 20.0%
!!! GATATTACTTTTATTTGAAG+AGG - chr3_4:22830419-22830438 Msa0494600:intron 20.0%
!!! TTTTTTTTCTCTCTCTTTTT+TGG + chr3_4:22830509-22830528 None:intergenic 20.0%
!!! ATTTTTTCTACAATGTTTAG+AGG + chr3_4:22830597-22830616 None:intergenic 20.0%
!!! AGATTAAATGTCTAGTTTTT+TGG + chr3_4:22830648-22830667 None:intergenic 20.0%
!! TTTCCAAATAATGTAAACAA+TGG - chr3_4:22830726-22830745 Msa0494600:intron 20.0%
!!! TTTGGTATTTGATTTATGAT+TGG + chr3_4:22830862-22830881 None:intergenic 20.0%
!!! TTTTAGTTAGAGAGAAATAT+TGG - chr3_4:22831070-22831089 Msa0494600:intron 20.0%
!! TTTAGTTAGAGAGAAATATT+GGG - chr3_4:22831071-22831090 Msa0494600:intron 20.0%
!!! TGGAAATAATGAAAAGTTTT+TGG + chr3_4:22831342-22831361 None:intergenic 20.0%
!! AATTGACATGACAATATTAT+TGG - chr3_4:22831545-22831564 Msa0494600:CDS 20.0%
!! CATTCAATTAATAATGTAAG+TGG + chr3_4:22831749-22831768 None:intergenic 20.0%
!! ATATTCCACAATAAATTTAG+TGG - chr3_4:22831819-22831838 Msa0494600:CDS 20.0%
!! TGTAACTCTATATGTTACTT+AGG - chr3_4:22829063-22829082 Msa0494600:intron 25.0%
! AGAACAATGGTTATATCTTT+AGG - chr3_4:22829257-22829276 Msa0494600:intron 25.0%
!!! CCGTTTTTGTGAAAATAAAT+TGG - chr3_4:22829402-22829421 Msa0494600:intron 25.0%
! ACGTAGATTATGAAAAAAAC+CGG - chr3_4:22829550-22829569 Msa0494600:intron 25.0%
! TAACAAAATTGTATCGTAAC+CGG + chr3_4:22829572-22829591 None:intergenic 25.0%
!!! GTTTGGATAATGCTTATTTT+GGG + chr3_4:22829654-22829673 None:intergenic 25.0%
! CAAAATAAGCATTATCCAAA+CGG - chr3_4:22829653-22829672 Msa0494600:intron 25.0%
! ACAATAGCAAACATAACTAA+GGG + chr3_4:22829680-22829699 None:intergenic 25.0%
!! CGTTTCTTTTTATTACAACA+TGG - chr3_4:22829731-22829750 Msa0494600:intron 25.0%
! GTATGTAAGAGTAACTAAAA+CGG - chr3_4:22830156-22830175 Msa0494600:intron 25.0%
! TATGTAAGAGTAACTAAAAC+GGG - chr3_4:22830157-22830176 Msa0494600:intron 25.0%
!!! TTTTAAGTGTAGTTCGAATA+CGG + chr3_4:22830227-22830246 None:intergenic 25.0%
!!! ACTCTAGGACATTTTTTTAA+AGG + chr3_4:22830360-22830379 None:intergenic 25.0%
!!! TTAAATGTCTAGTTTTTTGG+TGG + chr3_4:22830645-22830664 None:intergenic 25.0%
! ATTATTTGGAAAGAGAGAAA+CGG + chr3_4:22830718-22830737 None:intergenic 25.0%
! TAGCCATTGTTTACATTATT+TGG + chr3_4:22830732-22830751 None:intergenic 25.0%
!!! ATTTGATTTATGATTGGCTT+GGG + chr3_4:22830856-22830875 None:intergenic 25.0%
!! TATTTGATTTATGATTGGCT+TGG + chr3_4:22830857-22830876 None:intergenic 25.0%
! AGTGCATAAATGATTGTTAA+TGG - chr3_4:22830946-22830965 Msa0494600:intron 25.0%
! TCTTCTAATTTAGTCTATGT+TGG + chr3_4:22831199-22831218 None:intergenic 25.0%
!! AATTGTATTGAAGGTAGTTT+TGG + chr3_4:22831247-22831266 None:intergenic 25.0%
!!! ATAATGAAAAGTTTTTGGAG+AGG + chr3_4:22831337-22831356 None:intergenic 25.0%
! CGTTTCTGAATTAATAATTG+TGG + chr3_4:22831483-22831502 None:intergenic 25.0%
! ATGACAATATTATTGGATGA+CGG - chr3_4:22831552-22831571 Msa0494600:CDS 25.0%
! AACTGTTATGTGTTTAATTG+AGG + chr3_4:22831606-22831625 None:intergenic 25.0%
! CATTCAATTAATGATGTTTG+TGG - chr3_4:22831706-22831725 Msa0494600:intron 25.0%
TGTCAACTCTATAGTTCATA+AGG - chr3_4:22828886-22828905 Msa0494600:intron 30.0%
ACATATAGAGTTACAGAAGT+AGG + chr3_4:22829058-22829077 None:intergenic 30.0%
AACAAACACAGTAACATCAA+AGG + chr3_4:22829127-22829146 None:intergenic 30.0%
TTTCGAGCTTATTGTTTGTT+TGG - chr3_4:22829196-22829215 Msa0494600:intron 30.0%
! ATTTAGGGTTCGTTAGAATT+AGG - chr3_4:22829449-22829468 Msa0494600:intron 30.0%
! TTTTCACAATCTGCAATGAT+AGG + chr3_4:22829525-22829544 None:intergenic 30.0%
! TTGCGTAAACTGTTTTACAT+TGG - chr3_4:22829628-22829647 Msa0494600:intron 30.0%
! CGTTTGGATAATGCTTATTT+TGG + chr3_4:22829655-22829674 None:intergenic 30.0%
GACAATAGCAAACATAACTA+AGG + chr3_4:22829681-22829700 None:intergenic 30.0%
GTTGTAATAAAAAGAAACGG+AGG + chr3_4:22829730-22829749 None:intergenic 30.0%
AATGTGGTATAATAGAGAGA+AGG + chr3_4:22829801-22829820 None:intergenic 30.0%
GTTATCGGTAGATTAAAATG+TGG + chr3_4:22829817-22829836 None:intergenic 30.0%
GCTATATAATGGTCTATGAA+TGG + chr3_4:22830027-22830046 None:intergenic 30.0%
GTATTCGAACTACACTTAAA+AGG - chr3_4:22830226-22830245 Msa0494600:intron 30.0%
TGATTGCATTCAAATATCTC+AGG - chr3_4:22830314-22830333 Msa0494600:intron 30.0%
CAACACTTTATGTATACTCT+AGG + chr3_4:22830375-22830394 None:intergenic 30.0%
! TGTTGATGGACATCTATTAT+GGG + chr3_4:22830462-22830481 None:intergenic 30.0%
!! ATCAACACAAGAAGTGTTTA+GGG - chr3_4:22830475-22830494 Msa0494600:intron 30.0%
AAAATTAAGTTACAGCAGTG+AGG - chr3_4:22830555-22830574 Msa0494600:intron 30.0%
CTGTTCACTAATTGAATGAA+TGG - chr3_4:22830666-22830685 Msa0494600:intron 30.0%
! GAATGGAATTTTCCTTTTGT+TGG - chr3_4:22830683-22830702 Msa0494600:intron 30.0%
ATTTATCGCTCAAGATGATT+TGG + chr3_4:22830756-22830775 None:intergenic 30.0%
! TTTGTGTCTTGTGTCTTTTA+TGG + chr3_4:22830890-22830909 None:intergenic 30.0%
!!! TATCAACTTTTTTGGGAGTT+TGG - chr3_4:22831033-22831052 Msa0494600:intron 30.0%
GGAATAAAGGAAGTAACAAA+TGG + chr3_4:22831362-22831381 None:intergenic 30.0%
!! AGGGCTTTAATTTGGAATAA+AGG + chr3_4:22831375-22831394 None:intergenic 30.0%
! TGTCCCTTTTGTAACTATAA+CGG + chr3_4:22831404-22831423 None:intergenic 30.0%
TGTGTTTAATTGAGGTACAT+TGG + chr3_4:22831598-22831617 None:intergenic 30.0%
CAATTAATGATGTTTGTGGT+TGG - chr3_4:22831710-22831729 Msa0494600:intron 30.0%
! TTTGTGGTTGGTTCATAATA+TGG - chr3_4:22831722-22831741 Msa0494600:intron 30.0%
TTTATGCATTGAGAACAAGA+CGG + chr3_4:22831847-22831866 None:intergenic 30.0%
!! TAAACAATCCTTCTGAGATC+AGG - chr3_4:22828801-22828820 Msa0494600:CDS 35.0%
! CATAACCGACAATTTTGTCA+CGG + chr3_4:22828868-22828887 Msa0494590:CDS 35.0%
TAAGCTCGAAATTTCTTCCT+CGG + chr3_4:22829187-22829206 None:intergenic 35.0%
TGTTTGTTTGGAAAGACCTT+AGG - chr3_4:22829208-22829227 Msa0494600:intron 35.0%
GTTTGTTTGGAAAGACCTTA+GGG - chr3_4:22829209-22829228 Msa0494600:intron 35.0%
AAACGGGCAACAAAAGAAAA+AGG + chr3_4:22829388-22829407 None:intergenic 35.0%
!! TGGCTAGTTTGGTAGTATTT+AGG - chr3_4:22829433-22829452 Msa0494600:intron 35.0%
!! GGCTAGTTTGGTAGTATTTA+GGG - chr3_4:22829434-22829453 Msa0494600:intron 35.0%
GCTATTGTCTCATTACAACA+TGG - chr3_4:22829692-22829711 Msa0494600:intron 35.0%
! GTGTTGATGGACATCTATTA+TGG + chr3_4:22830463-22830482 None:intergenic 35.0%
CTAAACACTTCTTGTGTTGA+TGG + chr3_4:22830476-22830495 None:intergenic 35.0%
!! CATCAACACAAGAAGTGTTT+AGG - chr3_4:22830474-22830493 Msa0494600:intron 35.0%
AGAGCTTAACAAAGAGCAAA+AGG - chr3_4:22830531-22830550 Msa0494600:intron 35.0%
!!! TTGCATCTTCGTCATTTTTG+TGG + chr3_4:22830803-22830822 None:intergenic 35.0%
CGAAGATGCAATAAAAGACT+CGG - chr3_4:22830812-22830831 Msa0494600:intron 35.0%
! GTGTCTTTTATGGCAATCTT+TGG + chr3_4:22830880-22830899 None:intergenic 35.0%
GAGAGAAATATTGGGAGAAA+TGG - chr3_4:22831079-22831098 Msa0494600:intron 35.0%
!! TGAAAAGTTTTTGGAGAGGA+GGG + chr3_4:22831333-22831352 None:intergenic 35.0%
!! ATGAAAAGTTTTTGGAGAGG+AGG + chr3_4:22831334-22831353 None:intergenic 35.0%
!! TTTTGTAACTATAACGGGAG+GGG + chr3_4:22831398-22831417 None:intergenic 35.0%
!! CTTTTGTAACTATAACGGGA+GGG + chr3_4:22831399-22831418 None:intergenic 35.0%
CTCCCGTTATAGTTACAAAA+GGG - chr3_4:22831398-22831417 Msa0494600:intron 35.0%
! GTCCCTTTTGTAACTATAAC+GGG + chr3_4:22831403-22831422 None:intergenic 35.0%
GTTATAGTTACAAAAGGGAC+AGG - chr3_4:22831403-22831422 Msa0494600:intron 35.0%
!! ATAATTGATAACGGTGTCGA+GGG + chr3_4:22831663-22831682 None:intergenic 35.0%
!! TATAATTGATAACGGTGTCG+AGG + chr3_4:22831664-22831683 None:intergenic 35.0%
! GTCTTGTTCTCAATGCATAA+AGG - chr3_4:22831846-22831865 Msa0494600:CDS 35.0%
ACAAATTCAGTGGCTATGAT+GGG + chr3_4:22831901-22831920 None:intergenic 35.0%
TACAAATTCAGTGGCTATGA+TGG + chr3_4:22831902-22831921 None:intergenic 35.0%
TGGATCTCTTACAAATTCAG+TGG + chr3_4:22831911-22831930 None:intergenic 35.0%
TACCACGTCTATGATAGCTA+CGG + chr3_4:22828961-22828980 None:intergenic 40.0%
AGCTATCATAGACGTGGTAT+CGG - chr3_4:22828962-22828981 Msa0494600:intron 40.0%
AAGCTCGAAATTTCTTCCTC+GGG + chr3_4:22829186-22829205 None:intergenic 40.0%
ACCTTAGGGTACGATATTCA+TGG - chr3_4:22829223-22829242 Msa0494600:intron 40.0%
ACCATGAATATCGTACCCTA+AGG + chr3_4:22829227-22829246 None:intergenic 40.0%
! ATGCATTCTGAGGAGATCAA+AGG - chr3_4:22829286-22829305 Msa0494600:intron 40.0%
! TGCATTCTGAGGAGATCAAA+GGG - chr3_4:22829287-22829306 Msa0494600:intron 40.0%
AACAAAAGAAAAAGGAGGCG+AGG + chr3_4:22829380-22829399 None:intergenic 40.0%
! TGGATCTTTCTTGGCTAGTT+TGG - chr3_4:22829422-22829441 Msa0494600:intron 40.0%
AACATAACTAAGGGTCCGTT+TGG + chr3_4:22829671-22829690 None:intergenic 40.0%
TTAGAGGCAAAACCCTTTGA+TGG + chr3_4:22830581-22830600 None:intergenic 40.0%
! CGGACGTATTTTCCAACAAA+AGG + chr3_4:22830698-22830717 None:intergenic 40.0%
!!! CTTCGTCATTTTTGTGGTCT+AGG + chr3_4:22830797-22830816 None:intergenic 40.0%
!!! TGATTGGCTTGGGTTTTCTT+CGG + chr3_4:22830846-22830865 None:intergenic 40.0%
TATCCTCAATACACCCACAT+CGG - chr3_4:22830921-22830940 Msa0494600:intron 40.0%
ATTTATGCACTAGCCGATGT+GGG + chr3_4:22830937-22830956 None:intergenic 40.0%
!!! CAACTTTTTTGGGAGTTTGG+TGG - chr3_4:22831036-22831055 Msa0494600:intron 40.0%
!! CCTTTTGTAACTATAACGGG+AGG + chr3_4:22831400-22831419 None:intergenic 40.0%
CCTCCCGTTATAGTTACAAA+AGG - chr3_4:22831397-22831416 Msa0494600:intron 40.0%
!! GAGGTACATTGGTAGTGTAA+AGG + chr3_4:22831587-22831606 None:intergenic 40.0%
GTGTCGAGGGAAAAATAAGA+AGG + chr3_4:22831650-22831669 None:intergenic 40.0%
!! CGGTGCCACTAAATTTATTG+TGG + chr3_4:22831827-22831846 None:intergenic 40.0%
TGAAGAAGCCTGATCTCAGA+AGG + chr3_4:22828812-22828831 Msa0494590:CDS 45.0%
! CAGTAACATCAAAGGCGTAC+TGG + chr3_4:22829119-22829138 None:intergenic 45.0%
!! GGTGCTGATGTTGAGAACAA+TGG - chr3_4:22829244-22829263 Msa0494600:intron 45.0%
TAGGAAGCAGATGCATTCTG+AGG - chr3_4:22829276-22829295 Msa0494600:intron 45.0%
TCTGAGGAGATCAAAGGGAA+CGG - chr3_4:22829292-22829311 Msa0494600:intron 45.0%
CGGGCAACAAAAGAAAAAGG+AGG + chr3_4:22829385-22829404 None:intergenic 45.0%
!!! CGTCATTTTTGTGGTCTAGG+AGG + chr3_4:22830794-22830813 None:intergenic 45.0%
CATTTATGCACTAGCCGATG+TGG + chr3_4:22830938-22830957 None:intergenic 45.0%
TGCGGCCGTGACAAAATTGT+CGG - chr3_4:22828860-22828879 Msa0494600:intron 50.0%
CGCTAAAATCCATCACGTCG+TGG - chr3_4:22828931-22828950 Msa0494600:intron 50.0%
CTCCGTAGCTATCATAGACG+TGG - chr3_4:22828956-22828975 Msa0494600:intron 50.0%
TTCCAAGTCCTCAATAGCGG+CGG + chr3_4:22829002-22829021 None:intergenic 50.0%
CCGTTCCAAGTCCTCAATAG+CGG + chr3_4:22829005-22829024 None:intergenic 50.0%
CCGCTATTGAGGACTTGGAA+CGG - chr3_4:22829002-22829021 Msa0494600:intron 50.0%
CGCTATTGAGGACTTGGAAC+GGG - chr3_4:22829003-22829022 Msa0494600:intron 50.0%
ACGGTGGTGCTTCTTGATCT+CGG - chr3_4:22829311-22829330 Msa0494600:intron 50.0%
CGAGGATTGCTCTCACAATC+AGG + chr3_4:22829362-22829381 None:intergenic 50.0%
TACAGCAGTGAGGCCATCAA+AGG - chr3_4:22830565-22830584 Msa0494600:intron 50.0%
ACAGCAGTGAGGCCATCAAA+GGG - chr3_4:22830566-22830585 Msa0494600:intron 50.0%
TAGCCGATGTGGGTGTATTG+AGG + chr3_4:22830927-22830946 None:intergenic 50.0%
! GTAACTATAACGGGAGGGGA+GGG + chr3_4:22831394-22831413 None:intergenic 50.0%
! TGTAACTATAACGGGAGGGG+AGG + chr3_4:22831395-22831414 None:intergenic 50.0%
GATCCAACAGCTCCTGTATC+AGG - chr3_4:22831925-22831944 Msa0494600:CDS 50.0%
ACCAACCACTGCATGAGCTG+CGG - chr3_4:22828842-22828861 Msa0494600:CDS 55.0%
TACGGAGAACCACGACGTGA+TGG + chr3_4:22828943-22828962 None:intergenic 55.0%
ACTTGTGACCGCCGCTATTG+AGG - chr3_4:22828991-22829010 Msa0494600:intron 55.0%
!! ACGAGCGCTGAGATGTACTC+AGG + chr3_4:22829163-22829182 None:intergenic 55.0%
GAGGAGATCAAAGGGAACGG+TGG - chr3_4:22829295-22829314 Msa0494600:intron 55.0%
CGGTGGTGCTTCTTGATCTC+GGG - chr3_4:22829312-22829331 Msa0494600:intron 55.0%
TCTTGATCTCGGGCGAGCAA+AGG - chr3_4:22829322-22829341 Msa0494600:intron 55.0%
TTGGAGAGGAGGGAGAGCTT+TGG + chr3_4:22831323-22831342 None:intergenic 55.0%
! GGGAGGGGAGGGCTTTAATT+TGG + chr3_4:22831383-22831402 None:intergenic 55.0%
GCACCTGATACAGGAGCTGT+TGG + chr3_4:22831931-22831950 None:intergenic 55.0%
ACAGCTCCTGTATCAGGTGC+TGG - chr3_4:22831931-22831950 Msa0494600:CDS 55.0%
TCAAGACCTCAGTCCCTCCA+TGG + chr3_4:22831965-22831984 None:intergenic 55.0%
GCAGCTCATGCAGTGGTTGG+TGG + chr3_4:22828843-22828862 Msa0494590:CDS 60.0%
GACCGCCGCTATTGAGGACT+TGG - chr3_4:22828997-22829016 Msa0494600:intron 60.0%
!! CCTGTATCAGGTGCTGGTGC+CGG - chr3_4:22831937-22831956 Msa0494600:CDS 60.0%
GCCGCAGCTCATGCAGTGGT+TGG + chr3_4:22828846-22828865 Msa0494590:CDS 65.0%
ACATCTCAGCGCTCGTCCCG+AGG - chr3_4:22829167-22829186 Msa0494600:intron 65.0%
CCGGCACCAGCACCTGATAC+AGG + chr3_4:22831940-22831959 None:intergenic 65.0%
CCTCAGTCCCTCCATGGCTC+CGG + chr3_4:22831959-22831978 None:intergenic 65.0%
CACGGCCGCAGCTCATGCAG+TGG + chr3_4:22828850-22828869 Msa0494590:CDS 70.0%
!! AGGTGCTGGTGCCGGAGCCA+TGG - chr3_4:22831945-22831964 Msa0494600:CDS 70.0%
! TGCTGGTGCCGGAGCCATGG+AGG - chr3_4:22831948-22831967 Msa0494600:CDS 70.0%
! GCTGGTGCCGGAGCCATGGA+GGG - chr3_4:22831949-22831968 Msa0494600:CDS 70.0%
CCGGAGCCATGGAGGGACTG+AGG - chr3_4:22831956-22831975 Msa0494600:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 22828776 22831984 22828776 ID=Msa0494600;Name=Msa0494600
chr3_4 mRNA 22828776 22831984 22828776 ID=Msa0494600-mRNA-1;Parent=Msa0494600;Name=Msa0494600-mRNA-1;_AED=0.80;_eAED=0.80;_QI=0|0|0|0.33|0.5|0.66|3|0|150
chr3_4 exon 22831736 22831984 22831736 ID=Msa0494600-mRNA-1:exon:5675;Parent=Msa0494600-mRNA-1
chr3_4 exon 22831495 22831609 22831495 ID=Msa0494600-mRNA-1:exon:5674;Parent=Msa0494600-mRNA-1
chr3_4 exon 22828776 22828864 22828776 ID=Msa0494600-mRNA-1:exon:5673;Parent=Msa0494600-mRNA-1
chr3_4 CDS 22831736 22831984 22831736 ID=Msa0494600-mRNA-1:cds;Parent=Msa0494600-mRNA-1
chr3_4 CDS 22831495 22831609 22831495 ID=Msa0494600-mRNA-1:cds;Parent=Msa0494600-mRNA-1
chr3_4 CDS 22828776 22828864 22828776 ID=Msa0494600-mRNA-1:cds;Parent=Msa0494600-mRNA-1
Gene Sequence

>Msa0494600

ATGATCGGCAGATTTGGTCTGGTTTTAAACAATCCTTCTGAGATCAGGCTTCTTCACGTCGCTGCCACCAACCACTGCATGAGCTGCGGCCGTGACAAAATTGTCGGTTATGTCAACTCTATAGTTCATAAGGAAACTAACGAGTGCAAGCATCACGCTAAAATCCATCACGTCGTGGTTCTCCGTAGCTATCATAGACGTGGTATCGGTACAAAACTTGTGACCGCCGCTATTGAGGACTTGGAACGGATGTATCATCCTGAGTACATCTCAGCGCTCGTCCCGAGGAAGAAATTTCGAGCTTATTGTTTGTTTGGAAAGACCTTAGGGTACGATATTCATGGTGCTGATGTTGAGAACAATGCTCCCATCATAGCCACTGAATTTGTAAGAGATCCAACAGCTCCTGTATCAGGTGCTGGTGCCGGAGCCATGGAGGGACTGAGGTCTTGA
Protein sequence

>Msa0494600

MIGRFGLVLNNPSEIRLLHVAATNHCMSCGRDKIVGYVNSIVHKETNECKHHAKIHHVVVLRSYHRRGIGTKLVTAAIEDLERMYHPEYISALVPRKKFRAYCLFGKTLGYDIHGADVENNAPIIATEFVRDPTAPVSGAGAGAMEGLRS*