AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0497200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0497200 MtrunA17_Chr3g0092141 88.144 194 21 1 1 194 1 192 1.06e-125 352
Msa0497200 MtrunA17_Chr4g0060761 53.889 180 66 6 5 175 101 272 2.13e-55 177
Msa0497200 MtrunA17_Chr3g0127001 49.107 112 51 1 61 172 154 259 9.46e-31 113
Msa0497200 MtrunA17_Chr1g0182631 47.321 112 53 1 61 172 127 232 9.79e-29 107
Msa0497200 MtrunA17_Chr5g0406901 36.719 128 73 2 47 167 188 314 1.67e-22 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0497200 AT1G18790.1 62.500 128 41 2 48 168 112 239 9.05e-51 165
Msa0497200 AT1G74480.1 59.130 115 43 1 54 164 128 242 4.02e-43 146
Msa0497200 AT5G66990.1 50.746 134 59 2 42 170 142 273 1.51e-41 142
Msa0497200 AT5G53040.1 55.340 103 40 2 66 168 153 249 3.19e-31 114
Msa0497200 AT4G35590.2 39.130 115 63 1 61 168 192 306 3.24e-19 84.0
Msa0497200 AT4G35590.1 39.130 115 63 1 61 168 242 356 4.21e-19 84.3

Find 36 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTTGTACATTCTTTATA+AGG 0.172964 3_4:+29492343 None:intergenic
AATTATTCAATGACAGACTA+TGG 0.286299 3_4:-29492681 None:intergenic
AACTTGATGAAAACACAAAA+AGG 0.320323 3_4:-29491449 Msa0497200:CDS
TCTCTAGCAGCTTGAGATAT+TGG 0.323623 3_4:+29492456 None:intergenic
AAGAAAAGGTGTAGGGATCT+TGG 0.330015 3_4:-29492408 Msa0497200:CDS
GCTCATGGTTATGAGGTTAA+TGG 0.347444 3_4:-29491385 Msa0497200:CDS
TACTTGTACATTCTTTATAA+GGG 0.359802 3_4:+29492344 None:intergenic
AGTTGATGCTGGATATTATT+CGG 0.383233 3_4:-29492619 Msa0497200:CDS
TCAATGACAGACTATGGTTA+TGG 0.408759 3_4:-29492675 Msa0497200:CDS
GGCTTACACATTTGAAGAAA+AGG 0.415724 3_4:-29492422 Msa0497200:CDS
CATTCTTTATAAGGGTTTGA+AGG 0.417292 3_4:+29492352 None:intergenic
TATGAGGTTAATGGAACAAC+AGG 0.443913 3_4:-29491376 Msa0497200:CDS
GCTTAGACAAGCTTGTTTCA+AGG 0.460994 3_4:-29491427 Msa0497200:CDS
CAGGAGTCTTCTCTCATTGA+AGG 0.474327 3_4:-29491357 Msa0497200:CDS
CTGCTAGAGACCTTAATGTT+GGG 0.489436 3_4:-29492443 Msa0497200:CDS
GAAAGAAATGAAGAAGGTAG+AGG 0.489935 3_4:-29491487 Msa0497200:CDS
ATGAATGTGATGAGAAGATA+AGG 0.493501 3_4:-29491530 Msa0497200:CDS
ATTGCAGAAAGAAATGAAGA+AGG 0.517898 3_4:-29491493 Msa0497200:CDS
AGAGAAGGCTCATGGTTATG+AGG 0.522137 3_4:-29491392 Msa0497200:CDS
GCTGCTAGAGACCTTAATGT+TGG 0.523622 3_4:-29492444 Msa0497200:CDS
AATGTGTAAGCCCAACATTA+AGG 0.530566 3_4:+29492433 None:intergenic
CACATTTGAAGAAAAGGTGT+AGG 0.540159 3_4:-29492416 Msa0497200:CDS
AGGCTCATTAATTTCCTATG+TGG 0.543250 3_4:+29492372 None:intergenic
GAATTCAAAGGTGGCCACAT+AGG 0.544869 3_4:-29492386 Msa0497200:CDS
ATGTGGAATGAAGTTGATGC+TGG 0.550987 3_4:-29492630 Msa0497200:CDS
TACTGGAGAAAATGTTGCAA+AGG 0.556786 3_4:-29492568 Msa0497200:CDS
AGGCTAATTACAAGAAGAGA+AGG 0.568624 3_4:-29491407 Msa0497200:CDS
TGTTGCAAAGGAAATTGTGA+AGG 0.572064 3_4:-29492556 Msa0497200:CDS
ACATTTGAAGAAAAGGTGTA+GGG 0.575896 3_4:-29492415 Msa0497200:CDS
TTATTGGTGGTGCAGGAGCA+AGG 0.586089 3_4:-29491555 Msa0497200:intron
TTACAAGAAGAGAAGGCTCA+TGG 0.590126 3_4:-29491400 Msa0497200:CDS
GTAGGGATCTTGGAATTCAA+AGG 0.603743 3_4:-29492398 Msa0497200:CDS
GTTGCAAAGGAAATTGTGAA+GGG 0.628143 3_4:-29492555 Msa0497200:CDS
GCATCAACTTCATTCCACAT+TGG 0.632538 3_4:+29492633 None:intergenic
TGAGAAGATAAGGAATGCTG+TGG 0.656309 3_4:-29491520 Msa0497200:CDS
GGGATCTTGGAATTCAAAGG+TGG 0.676630 3_4:-29492395 Msa0497200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATAACTCAAAAAATAT+GGG + chr3_4:29491669-29491688 None:intergenic 10.0%
!! TATTAATAACTCAAAAAATA+TGG + chr3_4:29491670-29491689 None:intergenic 10.0%
!!! ATATATTTTAGAAAAATATG+AGG - chr3_4:29492110-29492129 Msa0497200:intron 10.0%
!!! TTTTATTTATTAATTATTGG+TGG - chr3_4:29492415-29492434 Msa0497200:CDS 10.0%
!! ATTAATAACTCAAAAAATAT+GGG + chr3_4:29491669-29491688 None:intergenic 10.0%
!! TATTAATAACTCAAAAAATA+TGG + chr3_4:29491670-29491689 None:intergenic 10.0%
!!! ATATATTTTAGAAAAATATG+AGG - chr3_4:29492110-29492129 Msa0497200:intron 10.0%
!!! TTTTATTTATTAATTATTGG+TGG - chr3_4:29492415-29492434 Msa0497200:CDS 10.0%
!!! AAAAAGAAAATCATGATTTT+AGG + chr3_4:29492216-29492235 None:intergenic 15.0%
!!! AAAAAGAAAATCATGATTTT+AGG + chr3_4:29492216-29492235 None:intergenic 15.0%
!! TACTTGTACATTCTTTATAA+GGG + chr3_4:29491642-29491661 None:intergenic 20.0%
!! TTACTTGTACATTCTTTATA+AGG + chr3_4:29491643-29491662 None:intergenic 20.0%
!! TGTTAAAAGTTATAGCAAAA+AGG - chr3_4:29492072-29492091 Msa0497200:intron 20.0%
!!! TAGGTTAATTTTCACTATAA+GGG + chr3_4:29492136-29492155 None:intergenic 20.0%
!!! TTAGGTTAATTTTCACTATA+AGG + chr3_4:29492137-29492156 None:intergenic 20.0%
!!! GGAATTGAAAAATCAATTTT+AGG + chr3_4:29492155-29492174 None:intergenic 20.0%
!!! CTTTTTCTTACTAATTTTTG+TGG - chr3_4:29492230-29492249 Msa0497200:intron 20.0%
!! TACTTGTACATTCTTTATAA+GGG + chr3_4:29491642-29491661 None:intergenic 20.0%
!! TTACTTGTACATTCTTTATA+AGG + chr3_4:29491643-29491662 None:intergenic 20.0%
!! TGTTAAAAGTTATAGCAAAA+AGG - chr3_4:29492072-29492091 Msa0497200:intron 20.0%
!!! TAGGTTAATTTTCACTATAA+GGG + chr3_4:29492136-29492155 None:intergenic 20.0%
!!! TTAGGTTAATTTTCACTATA+AGG + chr3_4:29492137-29492156 None:intergenic 20.0%
!!! GGAATTGAAAAATCAATTTT+AGG + chr3_4:29492155-29492174 None:intergenic 20.0%
!!! CTTTTTCTTACTAATTTTTG+TGG - chr3_4:29492230-29492249 Msa0497200:intron 20.0%
!!! TGTAGTGAATTGTTTTAATG+TGG - chr3_4:29492276-29492295 Msa0497200:intron 25.0%
! AACTTGATGAAAACACAAAA+AGG - chr3_4:29492534-29492553 Msa0497200:CDS 25.0%
!!! TGTAGTGAATTGTTTTAATG+TGG - chr3_4:29492276-29492295 Msa0497200:intron 25.0%
! AACTTGATGAAAACACAAAA+AGG - chr3_4:29492534-29492553 Msa0497200:CDS 25.0%
! TTATGGTGATGCTAAAAATG+TGG - chr3_4:29491325-29491344 Msa0497200:CDS 30.0%
! AGTTGATGCTGGATATTATT+CGG - chr3_4:29491364-29491383 Msa0497200:CDS 30.0%
! TTTTCGATTAGCAACATTAC+TGG - chr3_4:29491398-29491417 Msa0497200:CDS 30.0%
! ACATTTGAAGAAAAGGTGTA+GGG - chr3_4:29491568-29491587 Msa0497200:intron 30.0%
CATTCTTTATAAGGGTTTGA+AGG + chr3_4:29491634-29491653 None:intergenic 30.0%
!!! CACAATGTGATTTTTTGGTT+TGG + chr3_4:29491759-29491778 None:intergenic 30.0%
!!! CACTACACAATGTGATTTTT+TGG + chr3_4:29491764-29491783 None:intergenic 30.0%
AAAAATCACATTGTGTAGTG+AGG - chr3_4:29491764-29491783 Msa0497200:intron 30.0%
!! TTATTAATTATTGGTGGTGC+AGG - chr3_4:29492421-29492440 Msa0497200:CDS 30.0%
ATGAATGTGATGAGAAGATA+AGG - chr3_4:29492453-29492472 Msa0497200:CDS 30.0%
ATTGCAGAAAGAAATGAAGA+AGG - chr3_4:29492490-29492509 Msa0497200:CDS 30.0%
!! TTTTCATCAAGTTGCAAATC+TGG + chr3_4:29492527-29492546 None:intergenic 30.0%
! TTATGGTGATGCTAAAAATG+TGG - chr3_4:29491325-29491344 Msa0497200:CDS 30.0%
! AGTTGATGCTGGATATTATT+CGG - chr3_4:29491364-29491383 Msa0497200:CDS 30.0%
! TTTTCGATTAGCAACATTAC+TGG - chr3_4:29491398-29491417 Msa0497200:CDS 30.0%
! ACATTTGAAGAAAAGGTGTA+GGG - chr3_4:29491568-29491587 Msa0497200:intron 30.0%
CATTCTTTATAAGGGTTTGA+AGG + chr3_4:29491634-29491653 None:intergenic 30.0%
!!! CACAATGTGATTTTTTGGTT+TGG + chr3_4:29491759-29491778 None:intergenic 30.0%
!!! CACTACACAATGTGATTTTT+TGG + chr3_4:29491764-29491783 None:intergenic 30.0%
AAAAATCACATTGTGTAGTG+AGG - chr3_4:29491764-29491783 Msa0497200:intron 30.0%
!! TTATTAATTATTGGTGGTGC+AGG - chr3_4:29492421-29492440 Msa0497200:CDS 30.0%
ATGAATGTGATGAGAAGATA+AGG - chr3_4:29492453-29492472 Msa0497200:CDS 30.0%
ATTGCAGAAAGAAATGAAGA+AGG - chr3_4:29492490-29492509 Msa0497200:CDS 30.0%
!! TTTTCATCAAGTTGCAAATC+TGG + chr3_4:29492527-29492546 None:intergenic 30.0%
CTAAAAATGTGGTTCCAATG+TGG - chr3_4:29491336-29491355 Msa0497200:CDS 35.0%
TACTGGAGAAAATGTTGCAA+AGG - chr3_4:29491415-29491434 Msa0497200:CDS 35.0%
TGTTGCAAAGGAAATTGTGA+AGG - chr3_4:29491427-29491446 Msa0497200:CDS 35.0%
GTTGCAAAGGAAATTGTGAA+GGG - chr3_4:29491428-29491447 Msa0497200:CDS 35.0%
AATGTGTAAGCCCAACATTA+AGG + chr3_4:29491553-29491572 None:intergenic 35.0%
GGCTTACACATTTGAAGAAA+AGG - chr3_4:29491561-29491580 Msa0497200:intron 35.0%
! CACATTTGAAGAAAAGGTGT+AGG - chr3_4:29491567-29491586 Msa0497200:intron 35.0%
AGGCTCATTAATTTCCTATG+TGG + chr3_4:29491614-29491633 None:intergenic 35.0%
CAAGTCGCAAAATTTAGAGA+TGG + chr3_4:29492176-29492195 None:intergenic 35.0%
! CATCTCTAAATTTTGCGACT+TGG - chr3_4:29492174-29492193 Msa0497200:intron 35.0%
!!! AAATTTTGCGACTTGGTAAG+CGG - chr3_4:29492181-29492200 Msa0497200:intron 35.0%
ATGTTTGCGCAAGCAAATTA+TGG + chr3_4:29492326-29492345 None:intergenic 35.0%
GAAAGAAATGAAGAAGGTAG+AGG - chr3_4:29492496-29492515 Msa0497200:CDS 35.0%
AGGCTAATTACAAGAAGAGA+AGG - chr3_4:29492576-29492595 Msa0497200:CDS 35.0%
TATGAGGTTAATGGAACAAC+AGG - chr3_4:29492607-29492626 Msa0497200:CDS 35.0%
CTAAAAATGTGGTTCCAATG+TGG - chr3_4:29491336-29491355 Msa0497200:CDS 35.0%
TACTGGAGAAAATGTTGCAA+AGG - chr3_4:29491415-29491434 Msa0497200:CDS 35.0%
TGTTGCAAAGGAAATTGTGA+AGG - chr3_4:29491427-29491446 Msa0497200:CDS 35.0%
GTTGCAAAGGAAATTGTGAA+GGG - chr3_4:29491428-29491447 Msa0497200:CDS 35.0%
AATGTGTAAGCCCAACATTA+AGG + chr3_4:29491553-29491572 None:intergenic 35.0%
GGCTTACACATTTGAAGAAA+AGG - chr3_4:29491561-29491580 Msa0497200:intron 35.0%
! CACATTTGAAGAAAAGGTGT+AGG - chr3_4:29491567-29491586 Msa0497200:intron 35.0%
AGGCTCATTAATTTCCTATG+TGG + chr3_4:29491614-29491633 None:intergenic 35.0%
CAAGTCGCAAAATTTAGAGA+TGG + chr3_4:29492176-29492195 None:intergenic 35.0%
! CATCTCTAAATTTTGCGACT+TGG - chr3_4:29492174-29492193 Msa0497200:intron 35.0%
!!! AAATTTTGCGACTTGGTAAG+CGG - chr3_4:29492181-29492200 Msa0497200:intron 35.0%
ATGTTTGCGCAAGCAAATTA+TGG + chr3_4:29492326-29492345 None:intergenic 35.0%
GAAAGAAATGAAGAAGGTAG+AGG - chr3_4:29492496-29492515 Msa0497200:CDS 35.0%
AGGCTAATTACAAGAAGAGA+AGG - chr3_4:29492576-29492595 Msa0497200:CDS 35.0%
TATGAGGTTAATGGAACAAC+AGG - chr3_4:29492607-29492626 Msa0497200:CDS 35.0%
GCATCAACTTCATTCCACAT+TGG + chr3_4:29491353-29491372 None:intergenic 40.0%
!! ATGTGGAATGAAGTTGATGC+TGG - chr3_4:29491353-29491372 Msa0497200:CDS 40.0%
TCTCTAGCAGCTTGAGATAT+TGG + chr3_4:29491530-29491549 None:intergenic 40.0%
CTGCTAGAGACCTTAATGTT+GGG - chr3_4:29491540-29491559 Msa0497200:CDS 40.0%
! AAGAAAAGGTGTAGGGATCT+TGG - chr3_4:29491575-29491594 Msa0497200:intron 40.0%
GTAGGGATCTTGGAATTCAA+AGG - chr3_4:29491585-29491604 Msa0497200:intron 40.0%
ATTGTGTAGTGAGGAGATCT+AGG - chr3_4:29491773-29491792 Msa0497200:intron 40.0%
TTGTGTAGTGAGGAGATCTA+GGG - chr3_4:29491774-29491793 Msa0497200:intron 40.0%
TGAGAAGATAAGGAATGCTG+TGG - chr3_4:29492463-29492482 Msa0497200:CDS 40.0%
! GCTTAGACAAGCTTGTTTCA+AGG - chr3_4:29492556-29492575 Msa0497200:CDS 40.0%
TTACAAGAAGAGAAGGCTCA+TGG - chr3_4:29492583-29492602 Msa0497200:CDS 40.0%
GCTCATGGTTATGAGGTTAA+TGG - chr3_4:29492598-29492617 Msa0497200:CDS 40.0%
GCATCAACTTCATTCCACAT+TGG + chr3_4:29491353-29491372 None:intergenic 40.0%
!! ATGTGGAATGAAGTTGATGC+TGG - chr3_4:29491353-29491372 Msa0497200:CDS 40.0%
TCTCTAGCAGCTTGAGATAT+TGG + chr3_4:29491530-29491549 None:intergenic 40.0%
CTGCTAGAGACCTTAATGTT+GGG - chr3_4:29491540-29491559 Msa0497200:CDS 40.0%
! AAGAAAAGGTGTAGGGATCT+TGG - chr3_4:29491575-29491594 Msa0497200:intron 40.0%
GTAGGGATCTTGGAATTCAA+AGG - chr3_4:29491585-29491604 Msa0497200:intron 40.0%
ATTGTGTAGTGAGGAGATCT+AGG - chr3_4:29491773-29491792 Msa0497200:intron 40.0%
TTGTGTAGTGAGGAGATCTA+GGG - chr3_4:29491774-29491793 Msa0497200:intron 40.0%
TGAGAAGATAAGGAATGCTG+TGG - chr3_4:29492463-29492482 Msa0497200:CDS 40.0%
! GCTTAGACAAGCTTGTTTCA+AGG - chr3_4:29492556-29492575 Msa0497200:CDS 40.0%
TTACAAGAAGAGAAGGCTCA+TGG - chr3_4:29492583-29492602 Msa0497200:CDS 40.0%
GCTCATGGTTATGAGGTTAA+TGG - chr3_4:29492598-29492617 Msa0497200:CDS 40.0%
GCTGCTAGAGACCTTAATGT+TGG - chr3_4:29491539-29491558 Msa0497200:CDS 45.0%
GGGATCTTGGAATTCAAAGG+TGG - chr3_4:29491588-29491607 Msa0497200:intron 45.0%
GAATTCAAAGGTGGCCACAT+AGG - chr3_4:29491597-29491616 Msa0497200:intron 45.0%
AGAGAAGGCTCATGGTTATG+AGG - chr3_4:29492591-29492610 Msa0497200:CDS 45.0%
CAGGAGTCTTCTCTCATTGA+AGG - chr3_4:29492626-29492645 Msa0497200:CDS 45.0%
GCTGCTAGAGACCTTAATGT+TGG - chr3_4:29491539-29491558 Msa0497200:CDS 45.0%
GGGATCTTGGAATTCAAAGG+TGG - chr3_4:29491588-29491607 Msa0497200:intron 45.0%
GAATTCAAAGGTGGCCACAT+AGG - chr3_4:29491597-29491616 Msa0497200:intron 45.0%
AGAGAAGGCTCATGGTTATG+AGG - chr3_4:29492591-29492610 Msa0497200:CDS 45.0%
CAGGAGTCTTCTCTCATTGA+AGG - chr3_4:29492626-29492645 Msa0497200:CDS 45.0%
!!! TTTTTAAAATGTTTTTTTTT+TGG - chr3_4:29491840-29491859 Msa0497200:intron 5.0%
!!! ACATTTTATTTATTAATTAT+TGG - chr3_4:29492412-29492431 Msa0497200:CDS 5.0%
!!! TTTTTAAAATGTTTTTTTTT+TGG - chr3_4:29491840-29491859 Msa0497200:intron 5.0%
!!! ACATTTTATTTATTAATTAT+TGG - chr3_4:29492412-29492431 Msa0497200:CDS 5.0%
!! TTATTGGTGGTGCAGGAGCA+AGG - chr3_4:29492428-29492447 Msa0497200:CDS 50.0%
!! TTATTGGTGGTGCAGGAGCA+AGG - chr3_4:29492428-29492447 Msa0497200:CDS 50.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 29491311 29492694 29491311 ID=Msa0497200;Name=Msa0497200
chr3_4 mRNA 29491311 29492694 29491311 ID=Msa0497200-mRNA-1;Parent=Msa0497200;Name=Msa0497200-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|199
chr3_4 exon 29492347 29492694 29492347 ID=Msa0497200-mRNA-1:exon:6765;Parent=Msa0497200-mRNA-1
chr3_4 exon 29491311 29491562 29491311 ID=Msa0497200-mRNA-1:exon:6764;Parent=Msa0497200-mRNA-1
chr3_4 CDS 29492347 29492694 29492347 ID=Msa0497200-mRNA-1:cds;Parent=Msa0497200-mRNA-1
chr3_4 CDS 29491311 29491562 29491311 ID=Msa0497200-mRNA-1:cds;Parent=Msa0497200-mRNA-1
Gene Sequence

>Msa0497200

ATGACAGACTATGGTTATGGTGATGCTAAAAATGTGGTTCCAATGTGGAATGAAGTTGATGCTGGATATTATTCGGATAAACATGTCTTTTCGATTAGCAACATTACTGGAGAAAATGTTGCAAAGGAAATTGTGAAGGGTAAGAGATGTAGAGAAGAGAATACTAATAGTGCAAGAATGCTAACAAAAGAAACAATTTCTGAGTACTTCTATGTGCCAATATCTCAAGCTGCTAGAGACCTTAATGTTGGGCTTACACATTTGAAGAAAAGGTGTAGGGATCTTGGAATTCAAAGGTGGCCACATAGGAAATTAATGAGCCTTCAAACCCTTATAAAGAATGTACAAGAGCAAGGTAATGAATGTGATGAGAAGATAAGGAATGCTGTGGAAGTATTGCAGAAAGAAATGAAGAAGGTAGAGGAAAAGCCAGATTTGCAACTTGATGAAAACACAAAAAGGCTTAGACAAGCTTGTTTCAAGGCTAATTACAAGAAGAGAAGGCTCATGGTTATGAGGTTAATGGAACAACAGGAGTCTTCTCTCATTGAAGGTGTTGATGATCACAAAAAGTCAAATTTCTTCTTAACATCATGTTAA

Protein sequence

>Msa0497200

MTDYGYGDAKNVVPMWNEVDAGYYSDKHVFSISNITGENVAKEIVKGKRCREENTNSARMLTKETISEYFYVPISQAARDLNVGLTHLKKRCRDLGIQRWPHRKLMSLQTLIKNVQEQGNECDEKIRNAVEVLQKEMKKVEEKPDLQLDENTKRLRQACFKANYKKRRLMVMRLMEQQESSLIEGVDDHKKSNFFLTSC*